I know this has been asked before but I cannot easily lay my hand on the information.
What is the current knowledge about the longevity of ink-jet prints (black and white and colour) from medium to low end ink-jets (eg Epson 640 or Phote EX, HP 710). How much difference does the paper make to the expected life of the print.
Thanks
Ian
Ian Hallett HortResearch Mt Albert Research Centre Private Bag 92 169 Auckland, New Zealand Fax 64-9-815 4201 Telephone 64-9-815 4200 EMail ihallett-at-hort.cri.nz
Ian Since these products are recent, their permanence in storage has not yet been tested. My Epson 800/1520 prints stored in the dark seem to be OK *so far*. However, I have noticed fading of displayed Epson 800 and 1520 prints on Epson Glossy Photo paper over a period of about 15 months. These prints are mounted on a boared in an internal corridor, exposed to fluorescent lighting only, and daylight exposure would no doubt further accelerate fading. The assumption should be that these madia are not of archival quality, and it would probably be unwise to sell the prints as artworks, for example.
You might like to know that Marrutt (of darkroom rotary door fame) market Lyson long-life inks and media for Epson ink-jet printers Information about this can be found on the following url
http://www.marrutt.com/
Photo buffs may relish the fact that black inks can now be purchased in selenium and sepia tones, in addition to neutral black. Media Available include 120 and 170 gsm Matt Paper, 180 gsm Gloss Paper Backlight film and fine art papers in different surfaces and grades.
I have no experience of these products, and would be interested to hear any users' observations on colour and print quality, etc. when using these products.
Yours sincerely Chris Jeffree
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I know this has been asked before but I cannot easily lay my hand } on the information. } } What is the current knowledge about the longevity of ink-jet prints } (black and white and colour) from medium to low end ink-jets (eg } Epson 640 or Phote EX, HP 710). How much difference does the } paper make to the expected life of the print. } } Thanks } } Ian } } Ian Hallett } HortResearch } Mt Albert Research Centre } Private Bag 92 169 } Auckland, New Zealand } Fax 64-9-815 4201 } Telephone 64-9-815 4200 } EMail ihallett-at-hort.cri.nz }
===================================================================== DR CHRIS JEFFREE BIOSEM - BIOLOGICAL SCIENCES EM FACILITY UNIVERSITY OF EDINBURGH Daniel Rutherford Building King's Buildings, Mayfield Road EDINBURGH, EH9 3JH, Scotland, UK Tel. #44 131 650 5345 FAX. #44 131 650 6563 Mobile 0410 585 401 email c.jeffree-at-ed.ac.uk SEM / TEM bookings sem-at-ed.ac.uk =====================================================================
} Date: Fri, 27 Aug 1999 08:39:03 -0400 } From: Larry Allard {l2a-at-ornl.gov} } Subject: Re: Plasma cleaner } To: "Erasmus, Willem (WJ)" {willem.erasmus-at-sasol.com} } Cc: microscopy-at-Sparc5.Microscopy.Com
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } WJ: } } How are your catalyst sample supported? If you disperse the powder over } "clean" holey carbon grids (which contain no residual plastic film from the } production process), by simply dipping the grid into the catalyst powder } and shaking off the excess, you should find that contamination is not a } problem. Typically, powder specimens dangling over holes in carbon films } do not offer enough surface over which to diffuse molecules that cause } contamination buildup under the beam. Of course, the other source of } contamination could be the microscope itself, but that's another story... } } We have found that a plasma cleaner for the TEM specimen/specimen holder } works very well in the instances that we have contamination problems with } fine probe analysis using our Hitachi HF-2000. Our cleaner happens to be a } Fischione, and a couple of minutes treatment generally suffices to cure the } problem. If you suspect that the grids are the source of the } contamination, you might want to clean a grid in the plasma cleaner prior } to deposition of the catalyst specimen. I would be surprised if these } efforts did not produce satisfactory results for you. } } Good luck... } } Larry
Please, be careful not to use the plasma cleaner on holey carbon grids as, to my experience, often the carbon film itself is removed.
Albert Dr. Albert Romano-Rodriguez Professor Titular Dept. of Electronics Faculty of Physics University of Barcelona c/ Marti i Franques, 1 E-08028 BARCELONA Spain tel: +34-93-402 90 69 (+34-93-402 11 47) FAX: +34-93-402 11 48 e-mail: romano-at-el.ub.es
You are quite right. I forgot to mention this...but it turns out that with our Fischione we can plasma-clean holey carbon films for 1-2 minutes and still preserve the film. I think it is gone after about 5 minutes under standard cleaning conditions however. It may be the case that with just an inert gas, say nitrogen, there would be some cleaning benefit also, with perhaps not as much effect on the carbon film, since oxygen is eliminated as the reactive component in the plasma.
Larry
} } Please, be careful not to use the plasma cleaner on holey carbon } grids as, to my experience, often the carbon film itself is removed. } } Albert } Dr. Albert Romano-Rodriguez } Professor Titular } Dept. of Electronics } Faculty of Physics } University of Barcelona } c/ Marti i Franques, 1 } E-08028 BARCELONA } Spain } tel: +34-93-402 90 69 (+34-93-402 11 47) } FAX: +34-93-402 11 48 } e-mail: romano-at-el.ub.es
Dr. Lawrence F. Allard Senior Research Staff Member High Temperature Materials Laboratory Oak Ridge National Laboratory 1 Bethel Valley Road Bldg. 4515, MS 6064 PO Box 2008 Oak Ridge, TN 37831-6064
} } What is the current knowledge about the longevity of ink-jet prints } (black and white and colour) from medium to low end ink-jets (eg } Epson 640 or Phote EX, HP 710). How much difference does the } paper make to the expected life of the print.
I can't speak for these specific printers, but archival inks and papers are available for the Epson printers ... e.g., see:
www.dygraphics.com ... or search the wwweb for "lysonic inks"
I have Jim Mancuso's (AMT) older system that used a Kodak 1K 8 bit camera. It has worked fine but is developing hick-ups due to age (purcased 5 years ago) and incompatability with new computer's hardware/software. I reviewed his new system at the MSA 99 meetings and was impressed with it's design (and adaptability to some of my current hardware) and ease of use. His responce to problems has usually been good and he now has additional people working for him so I imagin he won't have to respond to all the calls himself now. Jim has always been good at "working with you " to solve hardware/software, merging your equipment with his, and to come up with a system you can afford. Your lucky, I just wish some one would come up with some money to help me get a 2K system... heck, even one of the new 1K systems!
In the past I too have had problems with getting info from Gatan sales people. I did get a good responce from this last meeting on a specimen holder though.
To be fair, I also looked at Soft Imaging System's and it's design was also good and the poster showing a comparison of their cameras showed very detailed images. I don't know how their price compares with the others though.
As I mention with AMT's system, ask about how well anyones system now will upgrade their older systems so you don/t have to buy a whole system every couple of years. Good Luck.
Roberts, Walck, Grant and Zaluzec conducted some experiments on plasma cleaning crushed zirconolate on holey carbon grids (MRS Spring Meeting 1997) and found the technique to be quite useful. The sample was processe= d in pure argon for a short time which removed most of the contamination. = As the plasma cleaner had 2 independent gas inlets, they were able to perfor= m a final short cleaning with pure oxygen which improved the sample without=
damaging the film. They were also able to reduce the power on the system=
to better control the cleaning process. I suspect that you were using a fixed power machine with a single gas inlet and an argon/oxygen mixture. =
Using that configuration it would be easy to "over process" the sample an= d remove the holey carbon grid. If you still have a need to clean samples = on a holey carbon grid, I would suggest running with the argon and then switching the gas line to run with pure oxygen for a short time. If you cannot reduce the power on your unit, you will still want to be very careful not to over process the sample.
You can find more details on the cleaning of holey carbon grids on our we= b site at www.southbaytech.com/pc150.html. On that page you will find a lin= k to the relevant paper under "Materials Research Society Spring-97 Meeting= ". I also have several other papers on plasma cleaning, plasma trimming and=
plasma etching available (some of those are on the web site). I would be=
happy to send you copies of any or all of them if you have an interest.
DISCLAIMER: The above mentioned work was done with a South Bay Technology= , Inc. Plasma Cleaner. Consequently I have a vested interest in promoting=
Ann No. Nile red is a lipid stain, therefore will stain the wax (though it may have trouble penetrating it to any depth). Wax-embedded tissues will in any case have non-polymerized lipids extracted during processing, so the answer is that Nile red is really applicable only to fresh tissues, deembedded tissues where e.g the cuticle remains after processing - it stains cuticle v. well - or tissues embedded in very polar media (PEG?). It is great for confocal microscopy on live cells and tissues
Chris
} I assume that you are talking about wax embedded tissue. Would Nile red work on resin embedded tissue? } } Ann Fook } } Ann Fook Yang } EM Unit, } Eastern Cereal and Oilseed Research Centre, } Rm 2091, K.W. Neatby Bldg., } Central Farm, } Ottawa, Ontario, Canada K1A 0C6 } } Phone: 613-759-1638 } Fax; 613-759-1701 } } } } } "Chris Jeffree" {cjeffree-at-srv0.bio.ed.ac.uk} 08/29 2:17 PM } } } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } } I strongly recommend that you use the fluorescent lipid dye Nile } red instead of Sudan Black. Nile red partitions into lipids rendering } them intensely fluorescent. You may use either FITC or RITC filter } sets, as you prefer. What makes Nile red preferable to the Sudan } dyes is that there is virtually no fluorescence from the dye unless it } is in a lipid environment. Therefore the specimen may be mounted } in an aqueous solution containing a low concentration of the dye, } and de-staining the non-lipid parts of the specimen is completely } unnecessary. Furthermore, because the specimen is immersed in } a stock of fluorochrome, there is minimal photobleaching. } } Chris Jeffree } } Date sent: Fri, 27 Aug 1999 09:44:47 -1000 } To: Microscopy-at-sparc5.microscopy.com } } From: "Melany H. Chapin" {mchapin-at-ntbg.org} } Subject: Sudan Black B } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } I am looking for a formula and procedure to check plant material for the } } presence of lipids using Sudan Black B. The formula I am using now stains } } everything black. I am specifically checking palm flowers and fruits. Thank } } you in advance. Please reply to my email: } } } } mchapin-at-ntbg.org } } } } ________________________________________________________________________ } } } } Melany H. Chapin Herbarium (PTBG) } } Curator & Plant Records Manager ph: 808-332-7324 ext. 133 } } National Tropical Botanical Garden (NTBG) fax: 808-332-9765 } } P.O. Box 340 www.ntbg.org } } End of Papalina Road email: mchapin-at-ntbg.org } } Lawai, Kauai, Hawaii 96765 } } USA } } ___________________________________________________________________________ } } } } } } } } } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ } Dr Chris Jeffree } University of Edinburgh } Biological Sciences EM Facility } Daniel Rutherford Building } King's Buildings EDINBURGH EH9 3JH } Tel: +44 (0) 131 650 5345 } FAX: +44 (0) 131 650 6563 } } Inveresk Cottage, 26 Carberry Road, } Inveresk, Musselburgh, Midlothian EH21 8PR, UK } Tel. +44 (0) 131 665 6062 / Mobile 0410 585 401 } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ } }
===================================================================== DR CHRIS JEFFREE BIOSEM - BIOLOGICAL SCIENCES EM FACILITY UNIVERSITY OF EDINBURGH Daniel Rutherford Building King's Buildings, Mayfield Road EDINBURGH, EH9 3JH, Scotland, UK Tel. #44 131 650 5345 FAX. #44 131 650 6563 Mobile 0410 585 401 email c.jeffree-at-ed.ac.uk SEM / TEM bookings sem-at-ed.ac.uk =====================================================================
This is an informative web site I stumbled across last year with comments about ink jet printers, inks and links to digital imaging info. I just checked and it still exists. Updated March 1999.
} } } I know this has been asked before but I cannot easily lay my hand } on the information. } } What is the current knowledge about the longevity of ink-jet prints } (black and white and colour) from medium to low end ink-jets (eg } Epson 640 or Phote EX, HP 710). How much difference does the } paper make to the expected life of the print. } } Thanks } } Ian } } Ian Hallett } HortResearch } Mt Albert Research Centre } Private Bag 92 169 } Auckland, New Zealand } Fax 64-9-815 4201 } Telephone 64-9-815 4200 } EMail ihallett-at-hort.cri.nz }
James S. Romanow The University of Connecticut Physiology and Neurobiology Department Electron Microscopy Facility U-131 Storrs, CT 06269-2131 bsgphy3-at-uconnvm.uconn.edu 860 486-2914 voice 860 486-1936 fax
If your interested in cleaning Carbon support films, my experiences have shown that a pure Argon plasmsa works very well.
I have used this on holey carbon and lacey carbon films with a variety of materials (crushed powders, catalysts, etc...)
Processing time varies with the degree of contamination and power/geometry of the unit. I prefer to use low power (~ 10 W) and longer processing times (5-10 minutes) this way you don't run the risk of destroying the support film on a processing time which is too fast. Remember you can clean a sample many times in small increments. The experimental data indicates that successive cleaning in small increments is effective.
If a film is particuliarly dirty I might use a few minutes of pure Oxygen, but as mentioned by the previous postings, the Oxygen is very aggressive to the carbon support.
Having a unit that allows multiple gas sources is advantageous in this respect.
Nestor Your Friendly Neighborhood SysOp
Disclaimer: ANL holds the patent on Plasma cleaning for TEM/AEM/SEM specimens and receives royalities from commerical sales of this technology.
I have a client who wants to look at biofilms existing on middle ear mucosa biopsies. The principle constituents of the biofilm are Pseudomonas, Strep. pneumon., and Haem. influ. I have referred him to the U Fl. Tips and Tricks page, but I am sure I have seen a thread on biofilms; I just didn't save them. Does anybody remember when this thread appeared so I can go check the archives or do you have a proven protocol that works? I'm not sure if he wants them with or without capsules. There is a group at Montana St. who do this using a cryo stage apparently because they so many bacteria. TIA for your help. -- ============================================================= Bill Chissoe III Electron Microscopist University of Oklahoma 770 Van Vleet Oval Norman, Ok. 73019 E-mail: wchiss-at-ou.edu Ph. (405)325-4391 =============================================================
Hi everyone, Anyone (else) out there have a cryo-stage on the SEM that does service for "outsiders"? I have a project I may not be able to handle due to internal problems with our cryo system {EMSCOPE SP2000A} ie: vacuum leak somewhere in LN2 lines to the stage as well as sputter head lack of coating even with plasma evident in the prep. system. We modified our JEOL 5800LV SEM to fit the system and had it operational ... years ago ( {gulp} ). I am also swamped...ah love the university beginning-of-semester-you-must-do-all-my-microscopy-in-one-month time. =) Thanks!!!
Tracey
Tracey Pepper Supervisor Bessey Microscopy Facility Iowa State University ph: 515-294-3872 fax: 515.294.1337
I work with a research and development group that prototypes semiconductor processes. Presently, there is a need in my company for a critical point dryer that can accomodate 8" wafers, an exceptionally large chamber size from what I have seen so far. If anyone has any knowledge as to the expandability of critical point dryers, or a source from which such a dryer could be purchased, please let me know.
Thermal microscopy is getting to be big business. You can now do thermal studies with Environmental SEM (ESEM, from Phillips), AFM (ex: Digital Instruments, ThermoMicroscopes, and newcomer JEOL), and light microscopy (see Mettler for a variety of stages and inquire of Leica to see if they still make the elegant VacuTherm, a chamber which sits on an inverted metallograph and permits all sorts of thermal and atmospheric controls).
See also: American Lab, July, 1999 for my review of this sort of instrumentation from the recent PITTCON meeting. If you can't find acopy in the UK, just drop me an email and we'll send you a Xerox.
Best regards, Barbara Foster Consortium President Microscopy/Microscopy Education ...Educating microscopists for greater productivity.
125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com Visit our web site {http://www.MME-Microscopy.com/education} ****************************************************** MME is America's first national consortium providing customized on-site workshops in all areas of microscopy, sample preparation, and image analysis.
At 01:13 PM 9/1/99 -0500, Chengge JIAO wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I received many helpful recipes for preparing bacteria for SEM analysis. These are all very informative and useful. Some can be performed by me using what I have at hand.
However, I had subsequently asked for sources/suppliers of prepared specimen stubs for my SEM and wound up with only one potential source.
Am I correct in concluding that no one will prepare SEM specimens of bacteria on a fee basis? Thus, I have to do it myself? What about non-bacterial specimens? Mites, lice, etc.? I have to do these as well?
If no one will provide this service, this helps me justify the investment in the necessary coating, CPD and other equipment necessary to make these specimen preparations.
Seems rather odd that no one does this. I guess that this is reality in this regard.
University of Queensland Postdoctoral Research Fellow Department of Mining, Minerals and Materials Engineering and the Centre for Microscopy and Microanalysis
Applications are invited for the above position. The Department of Mining, Minerals and Materials Engineering (MMM) and the Centre for Microscopy and Microanalysis (CMM) are looking to expand our efforts in the examination of phase transformations in ceramic materials. In this regard, we are specifically interested in the olivine to spinel transformation that is thought to be responsible for the production of deep earthquakes.
To aid us in this project, we are seeking an experienced Electron Microscopist (transmission electron microscopy) with a postgraduate research degree (preferably a PhD) in either materials science, physics, chemistry or earth sciences. The candidate should also have some knowledge of basic crystallography.
This is a full-time position for up to two years and is externally funded via an ARC grant. The successful candidate will be jointly attached to the MMM and the CMM. Salary will be in the range of $41,199.15 - $45,910.62 per annum. We are looking to fill this position as soon as possible. For further information, including position description, selection criteria please contact Dr. John Drennan, Centre for Microscopy and Microanalysis, Telephone: 61-7-3365 6353, Fax 61-7-3365 4422. E-mail: j.drennan-at-mailbox.uq.edu.au.
Closing date for applications 24th September, 1999.
-- ************************************************************ Duncan Waddell (BSc) Senior Scientific Officer Centre for Microscopy and Microanalysis The University of Queensland. St. Lucia. Qld. 4072 Telephone: +61-7-3365-4216 Facsimile: +61-7-3365-4422 WWW: http://www.uq.edu.au/nanoworld/nanohome.html ************************************************************ Any opinion expressed is that of the writer, and not necessarily that of CMM or of the University. ************************************************************
I would like to thank everyone for the helpful, competent and sometimes friendly replies to my posting. I now have a few different options to start working on the preparation of my samples, and some new techniques to exploit too.
Thanks a lot
Massimo
Dr. Massimo Catalano member of the Boards of Directors of Italian Society for Electron Microscopy CNR-IME Campus Universitario Via Arnesano 73100 Lecce - ITALY tel: + 39 0832 322362 * fax: + 39 0832 325299 * email: massimo.catalano-at-ime.le.cnr.it http://www.ime.le.cnr.it http://www.ime.le.cnr.it/sime/sime.htm
* Please note that, effective June 1998, a zero has to be dialed right after the country code (39) before the city code (832).
Tracey Pepper wrote: ======================================================= Hi everyone, Anyone (else) out there have a cryo-stage on the SEM that does service for "outsiders"? I have a project I may not be able to handle due to internal problems with our cryo system {EMSCOPE SP2000A} ie: vacuum leak somewhere in LN2 lines to the stage as well as sputter head lack of coating even with plasma evident in the prep. system. We modified our JEOL 5800LV SEM to fit the system and had it operational ... years ago ( {gulp} ). I am also swamped...ah love the university beginning-of-semester-you-must-do-all-my- microscopy-in-one-month time. =) Thanks!!! ======================================================= Our firm was either the first or among the first independent laboratories, at least in the USA to offer a cryo-SEM service some very many years ago. 100% of our laboratory services business is for "outsiders". We now operate with an Oxford system interfaced to a JEOL Model 840 SEM. We have had experience over the years with just about all the various kinds of samples one might want to look at this way. We would be happy to quote you on your requirements. More information about our services can be found on our website given below.
Chuck
=================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President 1-(800)-2424-SPI Structure Probe, Inc. FAX: 1-(610)-436-5755 PO BOX 656 e-mail: cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
Look for us! ############################ WWW: www.2spi.com ############################ ==================================================
Prices of SEMs can vary from free (for an old "fixer-up") to over a half million -for top of the line equipment with all the accessory goodies/analytical attachments available.
I'd like to add Nanonics Imaging, Ltd. to your list of providers of Scanning Thermal Microscopy (AFM). While Nanonics is primarily known for NSOM applications, it provides capabilities in Thermal, ElectroChemical, and Chemical Scanning Probe Microscopy.
Sincerely, Dave Eglinton Eglinton Instruments Specializing in Advanced Technologies representing . . . Nanonics Imaging, Ltd
PO Box 2686 Kennebunkport, ME 04046 T: 800-673-2430; F: 207-967-8741; E: eglinton-at-int-usa.net
Barbara Foster wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Dear Chennge, } } Thermal microscopy is getting to be big business. You can now do thermal } studies with Environmental SEM (ESEM, from Phillips), AFM (ex: Digital } Instruments, ThermoMicroscopes, and newcomer JEOL), and light microscopy } (see Mettler for a variety of stages and inquire of Leica to see if they } still make the elegant VacuTherm, a chamber which sits on an inverted } metallograph and permits all sorts of thermal and atmospheric controls). } } See also: American Lab, July, 1999 for my review of this sort of } instrumentation from the recent PITTCON meeting. If you can't find acopy } in the UK, just drop me an email and we'll send you a Xerox. } } Best regards, } Barbara Foster } Consortium President } Microscopy/Microscopy Education ...Educating microscopists for greater } productivity. } } 125 Paridon Street Suite 102 Springfield, MA 01118 } PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com } Visit our web site {http://www.MME-Microscopy.com/education} } ****************************************************** } MME is America's first national consortium providing } customized on-site workshops in all areas of } microscopy, sample preparation, and image analysis. } } At 01:13 PM 9/1/99 -0500, Chengge JIAO wrote: } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } } } Hi, } } } } I am looking for the name and email address of companies who can make or } } supply the High Temp. Micro-scope and Liquid Metal Density Tester. } } } } Does anybody know above items, please send email to: } } } } c.jiao-at-bham.ac.uk } } } } Thanks. } } } } } } } } } }
We need to get information about CL Detectors for a grant proposal. The application has to be submitted within two days, and has just arrived to us.
Does anyone have any information about CL detector types and cost ? Any help would be much appreciated as it will help us to meet the deadline. Information directly from vendors would be very welcome.
Please respond directly to me off list.
Thanks,
Colin
Colin Reid, Electron Microscope Unit, Trinity College Dublin, Dublin 2, Ireland. Tel: 353-1-6081820 Fax: 353-1-6770438 email: creid-at-tcd.ie
Hello, Has anyone seen circles ~1.4 um diameter , after immunofluroescence in lipofectamine transfection of HeLa cells with the corressponding DNA? What are these structures and what might it colocalize with?
We need to clean our tantalum final (fixed) aperture in a Hitachi 2460 variable pressure SEM. Since they are quite expensive ($600-800) does anyone know of a safe and effective procedure to clean such disc apertures? We are considering heating in a vac evaporator using a tungsten boat. But this procedure is tricky.
Thanks,
John B.
#################################################################### John J. Bozzola, Ph.D., Director Center for Electron Microscopy 750 Communications Drive - MC 4402 Southern Illinois University Carbondale, IL 62901 U.S.A. Phone: 618-453-3730 Fax: 618-453-2665 Email: bozzola-at-siu.edu Web: http://www.siu.edu/departments/shops/cem.html ####################################################################
A colleague is getting ready to service two Porter Bloom MT2-B ultramicrotomes, and I would appreciate if someone would provide the name, phone or whatever contact information you may have of independent service engineers for these microtomes.
Thank you.
John B.
#################################################################### John J. Bozzola, Ph.D., Director Center for Electron Microscopy 750 Communications Drive - MC 4402 Southern Illinois University Carbondale, IL 62901 U.S.A. Phone: 618-453-3730 Fax: 618-453-2665 Email: bozzola-at-siu.edu Web: http://www.siu.edu/departments/shops/cem.html ####################################################################
Are these in the cytoplasm? Or all over the place (in/on cells and the coverslip)? I haven't used any of the "new and improved" lipid-based transfection systems, but we used to get lipid droplets in/around HeLa...the cytoplasm sometimes was packed with them!
Have you tried electroporating, FU-Gene, adenovirus, etc. instead of lipid? All are cleaner (in terms of background) in our hands and the efficiency is higher. BUT, again, I haven't tried lipofection lately.
No financial interest in any of the above methods (I should be so lucky!).
Tamara Howard CSHL
On Thu, 2 Sep 1999, MICHAEL DELANNOY wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } } Hello, } Has anyone seen circles ~1.4 um diameter , after immunofluroescence in } lipofectamine transfection of HeLa cells with the corressponding DNA? What } are these structures and what might it colocalize with? } } Thanks, } } Mike D } } }
Hi. I am running a freeze fracture experiment. I have been tried to get a double replica device for a while. We have a Cressington freeze fracture CFE-50 but we don't have a Cressington double replica kit. I was told that Cressington doesn't supply double replica kit anymore. I talked to Balzers distributor in NH this morning. It seemed they had no idea what I was talking about. Does anybody there know where I can get a double replica device which fits Cressington freeze fracture CFE-50? Any ideas and suggestions would be appreciated. Thanks.
Shanling Shi Unilever Research US 45 River Road Edgewater, NJ 07020 Email: Shanling.Shi-at-unilever.com
I also am looking for a someone with cryo-optical and possibly cryo-SEM capabilities, preferably in the Chicago area. This will be for a potential project looking at several polymer and wax-like materials. thanks, Brad Huggins, EM Lab BPAmoco Naperville, IL
} ---------- } From: Tracey M. Pepper[SMTP:tpepper-at-iastate.edu] } Sent: Wednesday, September 01, 1999 3:54 PM } To: microscopy-at-Sparc5.Microscopy.Com } Subject: cryo-sem } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hi everyone, } Anyone (else) out there have a cryo-stage on the SEM that does } service for } "outsiders"? I have a project I may not be able to handle due to internal } problems with our cryo system {EMSCOPE SP2000A} ie: vacuum leak somewhere } in LN2 lines to the stage as well as sputter head lack of coating even } with } plasma evident in the prep. system. We modified our JEOL 5800LV SEM to } fit } the system and had it operational ... years ago ( {gulp} ). I am also } swamped...ah love the university } beginning-of-semester-you-must-do-all-my-microscopy-in-one-month time. =) } Thanks!!! } } Tracey } } } } Tracey Pepper } Supervisor } Bessey Microscopy Facility } Iowa State University } ph: 515-294-3872 } fax: 515.294.1337 } }
} We need to clean our tantalum final (fixed) aperture in a Hitachi 2460 } variable pressure SEM. Since they are quite expensive ($600-800) does } anyone know of a safe and effective procedure to clean such disc apertures? } We are considering heating in a vac evaporator using a tungsten boat. But } this procedure is tricky.
Dear John, The Handbook of Chemistry and Physics says, "Tantalum is almost immune to chemical attack at temperatures below 150 [deg] C ... At high temperatures, tantalum becomes much more reactive." Heating in a vac- uum, where there are no chemicals to react, should be OK, but I'd be sure to let the aperture cool completely before airing the chamber, and I'd make sure all the parts in the evaporator were clean to begin with. I'd plasma- clean to get rid of organics, and use NH4OH for W-residue without heating the aperture. Yours, Bill
Hello everyone - Another lurker pokes his head up out of the foxhole. We are beginning or Fall Semester Biological TEM course and one of the women signed up for the course just discovered that she will be a mother in the fullness of time. It is my opinion - and only that, really- that a biological EM labe with four or five fixation/dehydration/infiltration/embedment cycles proceeding at all times, some in cacodylate buffer, some in s-collidine, or whatever, plus exposure to resin components, etc., is no place for a small human being that is developing very rapidly, even if the packaging is sturdy and intelligent. We have had two other cases in the remembered past where this was a question and both women made an informed decision to stay in the class, against the advice of both the lab director and me. Both delivered healthy, full term babies with no problems (one of the "babies" is at least 13 years old now).
What do the rest of you think is the best course of action? Have there been decent studies of EM lab associated teratogens? Since I have never been a parent myself, this is one of those health hazard questions that I have simply decided to be very conservative about - but I have no hard and fast data that back me up.
Thanks very much for any help - data OR informed opinion that the group comes up with.
William P. Sharp Arizona State University Dept. Plant Biology, box 871601 Tempe, AZ 85287-1601 Phone - (602)-965-3210 Fax - (602)-965-6899
} Fall Semester Biological TEM course and one of the women signed up for the } course just discovered that she will be a mother in the fullness of time. } It is my opinion - and only that, really- that a biological EM labe with } four or five fixation/dehydration/infiltration/embedment cycles proceeding } at all times, some in cacodylate buffer, some in s-collidine, or whatever, } plus exposure to resin components, etc., is no place for a small human } being that is developing very rapidly, even if the packaging is sturdy and } intelligent.
If you assembled the MSDS's on all the chemicals used for bio- logical specimen preparation, I'm sure you would be horrified at their potential for harm. Given that ethanol, perhaps the least harmful of the organic solvents, can cause problems--fetal alcohol syndrome if there is enough exposure--I'd be extremely cautious with the more harmful chemicals. Also, many of the chemicals have the specific pur- pose of denaturing (fixing) biological material. The only saving grace--if one can call it that--is that some of these chemicals are so reactive that they will never get as far as the fetus. In this case, they would just damage the mother directly; damage to the fetus would be indirect. Have the woman in question read all the MSDS's before deciding. Remember also that the radiation exposure limits for preg- nant women are at least an order of magnitude lower than for the rest of the population, so the same might be expected for chemical exposure.
} We have had two other cases in the remembered past where this } was a question and both women made an informed decision to stay in the } class, against the advice of both the lab director and me. Both delivered } healthy, full term babies with no problems (one of the "babies" is at least } 13 years old now). } A very small N, and the control experiment was not done. We do not know how much subtle damage was inadvertantly done. Since Pb in very small amounts will lower the IQ a small, but measurable amount, it can be assumed that other environmental insults can also have small effects. Would the expectant mother want to take a year off her child's life expectancy? Increase the chances of cancer by 1%? Such potential problems are not possible to assess from the fact that one child has survived to adolescence.
} What do the rest of you think is the best course of action? Have there been } decent studies of EM lab associated teratogens? Since I have never been a } parent myself, this is one of those health hazard questions that I have } simply decided to be very conservative about - but I have no hard and fast } data that back me up.
I'd (again) get the MSDS's, look up the referrences listed on them, show all this to the woman, and let her decide. But, make sure she makes a fully informed decision. Yours, Bill Tivol
we are exploring the use of our new on-axis CCD camera on our Philips CM200 200 kV FEGTEM.
High resolution images are excellent but we have difficulty with low magnification modes and with capturing diffraction patterns due to the small field of view and the dynamic range in the pattern.
We are interested in conventional and convergent beam patterns.
If anyone out there has solved these problems please respond.
Thanks,
***************************************************** Mel Dickson, Deputy Director. Electron Microscope Unit, University of New South Wales. Sydney NSW 2052 Australia
A colleague of mine is exploring the purchase of a used SEM with (or without) EDS. They would prefer a more compact design but would consider others (such as a JEOL 840, for instance). If anyone out there has a system that they've been thinking of getting rid of, and it is in working condition, please contact me directly at the email address shown below. Think of what a good excuse this would be to upgrade your current system!
Cheers, JSV *************************** John Vetrano Pacific Northwest National Laboratory MSIN P8-16 P.O. Box 999 Richland, WA 99352 Phone: (509)372-0724 Fax: (509)376-6308 Email: mailto:john.vetrano-at-pnl.gov
I have problems with sintering B4C and stabilized zirconia together to obtain dense ceramic materials. A lower processing temperature (e.g., 1500=9AC) is favorable and the reaction between B4C and zirconia should be avoided. I sintered the compact pellet of B4C/ZrO2 mixed powders either in air at 1450=9AC (oxidation was insignificant) or in a hot isostatic press a= t 1250=9AC. But the products were very porous. Any suggestions are welcome an= d appreciated.
Weiliang Gong
Center for Radioactive Waste Management Advanced Materials Laboratory University of New Mexico 1001 University Blvd. SE Albuquerque, NM 87106 (505) 272-7142 (O) (505) 2727304 (FAX) } wgong-at-unm.edu (email address)
William, This issue has been addressed in our laboratories, where there is risk of infection as well as hazardous chemical exposure. As long as all standard precautions are observed and PPE is available for each task, there should be no exposure. All hazards must be addressed in your written procedures and safety criteria must be enforced. Make sure that your fume hood is certified and used correctly, and that gloves are chemical resistant and fit properly. Your institution should have a dept. of environmental and occupational safety and health. They can be a good source for information and policies. Bob Santoianni Emory University Hospital Atlanta, GA
John: For many years we have had Bill McGee of MTS service our Porter Bloom MT2-B ultramicrotomes and can recommend him for the prompt and always satisfactory service. He can be reached by phone at (315) 451-1404. His address is Microtome Service Co. 7568 Florian Way Liverpool, NY 13088 ######################################################## Henry Eichelberger, Manager Electron Microscopy Facility Department of Biological Sciences Binghamton University Binghamton, NY 13902-6000
Gloves, and a fume hood and some common sense are all that is needed
At 02:29 PM 9/2/1999 -0700, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} { GO GATORS Scott D. Whittaker 218 Carr Hall EM Technician Gainesville, FL 32610 University Of Florida ph 352-392-1184 ICBR EM Core Lab fax 352-846-0251 sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/ The home of " Tips & Tricks "
Bill: I would like to add to your comments. A not uncommon mistake when cleaning moly or tantalum apertures is overheating, because we all know that these metals have very high melting points and so white-heat seem alright. Unfortunately high heat leads to large crystal formation and the aperture becomes useless after a couple of heating cycles. I used to clean moly and tantalum apertures on a moly (or tungsten) boat. This too should be previously heated for cleaning. Apertures may be stored in a strong solvent and rinsed in ethanol and than water prior to heat cleaning. I suppose the plasma ashing may do that better. Heat cleaning should be for a couple of minutes, but only at a cherry red heat. Leave a couple of minutes to cool before venting the system.
I don't know that aperture, but the appear rather pricey - has John tried to get it trough another supplier? Most suppliers can get just about any aperture made to order, at usually lower prices than EM manufacturer's apertures. Disclaimer: ProSciTech supplies apertures. Cheers Jim Darley ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com.au Great microscopy catalogue, 500 Links, MSDS, User Notes www.proscitech.com.au
On Friday, September 03, 1999 6:29 AM, William Tivol [SMTP:tivol-at-wadsworth.org] wrote: } } John J. Bozzola wrote: } } } We need to clean our tantalum final (fixed) aperture in a Hitachi 2460 } } variable pressure SEM. Since they are quite expensive ($600-800) does } } anyone know of a safe and effective procedure to clean such disc apertures? } } We are considering heating in a vac evaporator using a tungsten boat. But } } this procedure is tricky. } } Dear John, } The Handbook of Chemistry and Physics says, "Tantalum is almost } immune to chemical attack at temperatures below 150 [deg] C ... At high } temperatures, tantalum becomes much more reactive." Heating in a vac- } uum, where there are no chemicals to react, should be OK, but I'd be sure } to let the aperture cool completely before airing the chamber, and I'd make } sure all the parts in the evaporator were clean to begin with. I'd plasma- } clean to get rid of organics, and use NH4OH for W-residue without } heating the aperture. } Yours, } Bill }
Recording diffraction patterns using 12 bit dynamic range or more should preserve sufficient information in the pattern, though it may be invisible to the human eye. Hence, you'll need to perform some image analysis in order to filter out the bell-shaped background. See e.g.
S. Zaefferer and R. A. Schwarzer Z. Metallk. 85 (1994), p585.
A customized scintillator (or fluorescent screen) for the camera may be used in order to reduce the strong transmitted beam intensity. Alternatively, a customized beam-stop may be used.
The magnification problem may be solved by recording overlapping images from different parts of the diffraction pattern and then stitch them together using image correlation matching. This procedure can be fully automated, though it will require some efforts in software development (CM remote control and image processing). I believe the CM200 is capable of imaging diffraction patterns at very low camera lengths. It may therefore be a better option to reduce the distortion in these patterns by applying a suitable deconvolution procedure.
Cheers, Paul
=================== Paul Baggethun Alcoa Technical Center Alcoa Center, PA 15069 USA =================== + 724 - 337-1760 (tel) + 724 - 337-2044 (fax) ===================
NOTE: The opinions expressed here represent the opinions of the author and do not necessarily represent the opinions of those who hold other opinions.
} ---------- } From: Melvyn Dickson[SMTP:M.Dickson-at-unsw.edu.au] } Sent: Thursday, September 02, 1999 6:47 PM } To: microscopy-at-Sparc5.Microscopy.Com } Subject: Diffraction patterns with on-axis CCD camera } } Dear All, } } we are exploring the use of our new on-axis CCD camera on our Philips } CM200 } 200 kV FEGTEM. } } High resolution images are excellent but we have difficulty with low } magnification modes and with capturing diffraction patterns due to the } small field of view and the dynamic range in the pattern. } } We are interested in conventional and convergent beam patterns. } } If anyone out there has solved these problems please respond. } } Thanks, } } } ***************************************************** } Mel Dickson, } Deputy Director. } Electron Microscope Unit, } University of New South Wales. } Sydney NSW 2052 Australia } } Phone (+612) 9385-6383 } Fax (+612) 9385-6400 } Website {http://srv.emunit.unsw.edu.au} } ***************************************************** }
Hi there, does anyone know of a supplier of Teledyne Hastings=20 thermocouple gauges in the MidWest? I ma looking for a replacement=20 for a DV-23 Vacuum Gauge Tube from a Gatan Ion Miller. I can buy=20 this part from Gatan, but I was wondering if I could avoid paying any=20 markup by going a more direct route. Any info would be appreciated
Please note new FAX number.
John Mansfield PhD CPhys MInstP North Campus Electron Microbeam Analysis Laboratory 417 SRB, University of Michigan 2455 Hayward, Ann Arbor MI 48109-2143 Phone: (734) 936-3352 FAX (734) 763-2282 Cellular Phone: (734) 358-7555 Email: jfmjfm-at-engin.umich.edu URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html Location: Lat. 42=B0 16' 48" Long. 83=B0 43' 48"
I have been trying in vain for the past two weeks to download the most recent version of UTHSCSA's ImageTool program. The two links I have been trying from a variety of approaches are: http://ddsdx.uthscsa.edu and ftp://maxrad6.uthscsa.edu. Are these still valid, are there more current links or is the software no longer available ? Thanks for any information on this matter. Regards,
Andrew Ochalski, Microscopy Technician, Dept. of Biology, University of Ottawa Room 108, Gendron Bldg. 130 Louis Pasteur, Ottawa, Ontario CANADA K1N 6N5 613-562-5800 x6343 FAX: 613-562 5486
i am interested in etching thin sputtered palladium films approx. 500-1000 angstroms thick. would you please assist me in finding the appropriate chemical to etch with. i would like to have an etch time less than one minute.
As you know Ladd manufactures and cleans thousands of moly and tantalum apertures for numerous applications besides electron microscopes. Some of those applications, such as for satllites, x-ray or medical equipment, require ultra-clean apertures where even the appearance of transient debris is a problem.
In the hope that some of our experience might be of benefit let me tell you what we do:
1. We have a proprietary chemical pre-cleaning process after the aperture is manufactured.
2. We then heat the aperture in a tungsten boat in our evaporator. We feel it's critical in this process to: a) Heat only till cherry red for approximately 5 minutes. b) Turn the current up slowly and back down slowly. c) Be sure the aperture returns to its normal color before venting to air.
3. We will also store and ship in acid cleaned containers.
Hope this helps in some way.
Disclaimer: Ladd does manufacture and sell evaporators and apertures in a variety of materials for electron microscopes.
John Arnott -- LADD RESEARCH 13 Dorset Lane Williston, VT 05495 TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere) FAX 1-802-878-8074 e-mail ladres-at-worldnet.att.net web site http://www.ladd.cc
I am looking for an instruction manual for an Reichert-Jung model ''Histoslide 2000'' slinding Microtome. Any suggestions? Thanks. I look forward to your responses.
JOSE ULLAN SERRANO --------------------------- Departamento de ANATOMIA Universidad Panamericana c/ Donatello, 59 Col. Insurgentes-Mixcoac 03920 MEXICO, D.F.
Hello, I have a couple echants you might want to try. 1) Aqua Regia in Glycerol, used cold. I am not sure of the ratios. 2) Another fast etch is hot Nitric Acid. I am sure there are other etches but many employ some fairly noxious chemicals. Here is an example of a slow etch which can be speeded up with the addition of a 3% solution of Sodium Iodide. Potassium Cyanide 20% in water and Amonium Persulphate 20% in water.
Good luck and be careful. This information was gleaned from some dusty old notes.
Alex Greene SCIENTIFIC INSTRUMENTATION SERVICES, INC. PMB-499, 1807 West Slaughter Lane, Number 200 Austin, Texas 78748-6200 Phone: 512/282-5507 FAX 512/280-0702
QUALITY ELECTRON MICROSCOPE SERVICE -----Original Message----- } From: Corcelius, Brian {BCorcelius-at-krautkramer.com} To: 'microscopy-at-msa.microscopy.com' {microscopy-at-Sparc5.Microscopy.Com}
The Imaging Technology Group at the Beckman Institute, University of Illinois at Urbana-Champaign is seeking a research specialist in light microscopy who will be responsible for operation, maintenance and training for the light microscopes in the ITG microscopy suite.
Responsibilities will include:
=B7 Operating/maintaining light, fluorescence, confocal and stereology microscopes. =B7 Application development: Work in conjunction with users to appl= y new LM techniques to their research. =B7 Supervise and train others in the use of the light microscopy instruments. =B7 Develop novel applications to take advantage of the unique capabilities of the instruments.
More information on facilities and instrumentation can be found at http://www.itg.uiuc.edu/
This position requires a Bachelor's degree with experience in all aspects of light microscopy (specimen preparation, imaging, data analysis). Desirable skills would include experience with data analysis packages for light microscopy.
This is a 12-month, 100% time regular appointment with standard university benefits. Salary will be commensurate with education and experience. At this time, the position is available for a period of three years. The position is available immediately. In order to ensure full consideration, applications must be received by September 21, 1998. Please send letter of application and resume to:
Tricia Ware Imaging Technology Group Beckman Institute for Advanced Science and Technology 405 N. Mathews Urbana, IL 61801 (217)244-0170 e-mail: pware-at-uiuc.edu
The University of Illinois is an Affirmative Action/Equal Opportunity Employer. Women and minorities are encouraged to apply.
Stephen Rogers University of California at Davis Section of Molecular and Cellular Biology Briggs Hall, Room 149 Davis, CA 95616 tel: 530.752.2273 email: steverog-at-life.uiuc.edu
Dear John, According to my sources in Hitachi Service Canada, this should be a Molybdenum aperture and should be cleaned by the standard high-vacuum, cherry-red heat in a Mo boat. The Mo boat should be heated empty first to clean it. This method should also work on a tantalum aperture. At 02:22 PM 9/2/99 -0500, you wrote:
} } We need to clean our tantalum final (fixed) aperture in a Hitachi 2460 } variable pressure SEM. Since they are quite expensive ($600-800) does } anyone know of a safe and effective procedure to clean such disc apertures? } We are considering heating in a vac evaporator using a tungsten boat. But } this procedure is tricky. } } Thanks, } } John B. } Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
Vetrano, John S wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Hi All; } } A colleague of mine is exploring the purchase of a used SEM with (or without) } EDS. They would prefer a more compact design but would consider others (such as } a JEOL 840, for instance). If anyone out there has a system that they've been } thinking of getting rid of, and it is in working condition, please contact me } directly at the email address shown below. Think of what a good excuse this } would be to upgrade your current system! } } Cheers, JSV } *************************** } John Vetrano } Pacific Northwest National Laboratory } MSIN P8-16 } P.O. Box 999 } Richland, WA 99352 } Phone: (509)372-0724 Fax: (509)376-6308 } Email: mailto:john.vetrano-at-pnl.gov
We sell SEM's, TEM's and related Instruments. Available: AMRAY 1200 $4,900.00 1200B 9,000.00 1700, LaB6/W, turbo pumped $19,000.00 Vacuum Evaporators, Sputter coaters, Crit. Point Dryers and other equipment. Regards, Markus F. Meyenhofer Microscopy Labs
John F. Mansfield wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Hi there, does anyone know of a supplier of Teledyne Hastings } thermocouple gauges in the MidWest? I ma looking for a replacement } for a DV-23 Vacuum Gauge Tube from a Gatan Ion Miller. I can buy } this part from Gatan, but I was wondering if I could avoid paying any } markup by going a more direct route. Any info would be appreciated } } Please note new FAX number. } } John Mansfield PhD CPhys MInstP } North Campus Electron Microbeam Analysis Laboratory } 417 SRB, University of Michigan } 2455 Hayward, Ann Arbor MI 48109-2143 } Phone: (734) 936-3352 FAX (734) 763-2282 } Cellular Phone: (734) 358-7555 } Email: jfmjfm-at-engin.umich.edu } URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html } Location: Lat. 42° 16' 48" Long. 83° 43' 48"
John,
You can buy DV-23 vac. gauge tube from Kurt J. Lesker Company (vacuum products): (800)245-1656 - lesker.com
Disclaimer: I have no business affiliation with the KJL Company other than being satisfied customer.
I think UTHSCA ImageTool was taken over by Scion Image(???) At any rate, Scion has an easy to use image analysis program that can be downloaded for free from their site:
http://scioncorp.com
Sometimes I have LUT problems, but not when I'm doing image analysis. (Before I got Photoshop, I used to use it to draw pictures ;-) )
-------------------------------------
On Fri, 3 Sep 1999, Andrew Ochalski wrote:
} } Dear Microscopists, } } I have been trying in vain for the past two } weeks to download the most recent version of } UTHSCSA's ImageTool program. The two links } I have been trying from a variety of approaches } are: } http://ddsdx.uthscsa.edu } and } ftp://maxrad6.uthscsa.edu. } Are these still valid, are there more current links } or is the software no longer available ? Thanks ..
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Shanling Shi: I know that double replica devices were available years ago. I still don't know why, so if you require one for your project, let me know. This is how I understand the FE technique and the replica device.
During the FE process a very cold blade removes a series of thin shavings from the sample frozen specimen, to expose a surface of an internal cross section. This results not in a smooth cut (on a microscopic scale) but follows lines of natural weakness, often over and under organelles. "Etching" in Freeze Etching, refers to a short time period, when the much colder knife (minus 170, versus the specimen at minus 100 degrees C) is "parked" above the specimen and some ice sublimes onto the knife, thus increasing relief. Subsequently the samples is coated with Pt/C at an angle and C to make the replica. After cleaning the replica is viewed. These replicas give a surprisingly detailed and somewhat three-dimensional impression of the specimen, which is not prepared using conventional fixation. Now - Shadows from the Pt coating are not electron dense and appear to us negative, meaning "hills and valleys" are reversed. For this reason FE images were often printed as negatives. If you were to prepare one negative and one positive print you would have the impression of the two matching sides, like from a double replica device.
The only reason for a double replica device I can think off, is that the fracture would be much coarser than the one exposed by conventional FE "shaving" method. With the event of high resolution SEM. which has partially replaced the need for the elegant, but difficult FE (TEM) method, there is basically no need for the double replica device. I saw no other posted reply on this topic, but would love to learn from users of the double replica device if there are any special merits today.
Disclaimer: ProSciTech handles some FE technique supplies, but not the apparatus. Cheers Jim Darley ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com.au Great microscopy catalogue, 500 Links, MSDS, User Notes www.proscitech.com.au
On Friday, September 03, 1999 3:54 AM, Shanling Shi [SMTP:Shanling.Shi-at-unilever.com] wrote: } } } Hi. I am running a freeze fracture experiment. I have been tried to get a } double replica device for a while. We have a Cressington freeze fracture CFE- } 50 } but we don't have a Cressington double replica kit. I was told that } Cressington } doesn't supply double replica kit anymore. I talked to Balzers distributor in } NH this morning. It seemed they had no idea what I was talking about. Does } anybody there know where I can get a double replica device which fits } Cressington freeze fracture CFE-50? Any ideas and suggestions would be } appreciated. Thanks. } } Shanling Shi } Unilever Research US } 45 River Road } Edgewater, NJ 07020 } Email: Shanling.Shi-at-unilever.com }
For what it's worth: I heard Gladys Fiedler speak at a meeting of the AAAS in 1991 about the topic of teratogens and motherhood, and interestingly, she said that the only known case at that time of working with teratogenic materials actually affecting a fetus was that of a man who had worked with heavy metals and his child was born with some kind of defect. At the time Dr. Fiedler was speaking, the government was making some rule that women of child-bearing age couldn't even work in certain jobs for fear that they might affect their offspring, and the point she was making - probably because, as I understand it, this was (is?) her area of study - was that teratogenic affects are perhaps even more likely to affect the sperm which is constantly being made than to affect the child in the uterus, unless of course the woman ingests the teratogen. Just a thought. Gayle Brosnan-Watters
Gayle Brosnan-Watters, Ph.D. Assistant Professor Psychology Department Vanguard University of Southern California 55 Fair Drive Costa Mesa, CA 92626 Phone 714-556-3610 Ext. 454 Fax 714-966-6316 GBrosnanWatters-at-vanguard.edu
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } } Dear Microscopists, } } I have been trying in vain for the past two } weeks to download the most recent version of } UTHSCSA's ImageTool program. The two links } I have been trying from a variety of approaches } are: } http://ddsdx.uthscsa.edu } and } ftp://maxrad6.uthscsa.edu. I had the same difficulty. I finally got it at http://macorb.uthscsa.edu/dig/itdesc.html.
I did not "trace" the freeze-fracture discussion. In my point of view, people used "double replicas" technique when for some reason they want to see complementary surfaces of the sample. Double replicas device was available many years ago from BALZERS. Simplest construction is two copper/brass small plates with joint. Plates may rotate by hard spring 180 degree in the manner of the book's cover when you open the book. In the start position, the plates are closed (like closed book) with sample between the plates (say pages in the book) and fixed in such position by some kind of "stopper". The "book" than quickly frozen and mounted in the vacuum evaporator. Vacuum evaporator equipped with some simple device to release "stopper" in time. After all necessary manipulation, "stopper" is released then plates are "opened" by force of the hard spring and breaks the frozen sample. With good luck, the sample broke in the middle than both plates exposure the part of sample with complementary surfaces. The surfaces are shadowed and so as usual. I think, such device is so easy to make in machine shop. Described "book"-like device was developed many years ago in the Institute of Biophysics, Pushchino, Russia. I don't know who was author of this device, but it was used in the Lab. Prof. Borovyagin. I newer used such device in my experiments but was frequent "witness" how people used that. If my description of the "device" is not clear (it is better to draw it), please, E.mail me and I will share with you all I know in this matter. If people from ListServer interested, I may prepare some drawing to illustrate the principle and sent it privately upon request.
Best regards,
} Date: Sun, 05 Sep 1999 22:42:05 +1000 } From: jim {jim-at-proscitech.com.au} } Subject: RE: Freeze fracture Need Help } To: 'Shanling Shi' {Shanling.Shi-at-unilever.com} , } "Microscopy-at-MSA.Microscopy.Com" {Microscopy-at-sparc5.microscopy.com} } Reply-to: "jim-at-proscitech.com.au" {jim-at-proscitech.com.au} } Organization: ProSciTech } X-Mailer: Microsoft Internet E-mail/MAPI - 8.0.0.4211 } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Dr. Sergey Ryazantsev Department of Biological Chemistry UCLA School of Medicine Box 951737 Los Angeles, CA 90095-1737 Phone: (310)825-1144 (Lab) FAX (departmental): (310) 206-5272 mailto: sryazant-at-ucla.edu mailto:sryazant-at-ucla.edu http://www.bol.ucla.edu/~sryazant E. mail: sryazant-at-ucla.edu http://www.ben2.ucla.edu/~sryazant
I have a problem obtaining diffraction patterns on our CCD camera. We have a Philips CM200 TEM fitted with a Gatan Image Filter. Due to an extra magnification factor introduced by the GIF the image on the TEM viewing screen is much smaller than the image seen on the computer screen. Although bothersome, this does not present too much of a problem in ordinary imaging.
However, when obtaining a diffraction pattern I find that one cannot shield the CCD camera from the centre spot (transmitted beam) with the beam-stop because the image of the beam-stop covers the diffraction pattern. The only thing visible on the screen is the beam-stop.
At the moment I am recording diffraction patterns on film, but is there a way to use the CCD camera for this purpose ?
Dear members of the list, I think I remember that some food researchers are on the list. They could help me or anyone of you with a better biochemical background or somebody who knows other sources which could help me on this question:
Who can give me some general information on caramels and caramelization? What influences the fluidity/viscosity of caramel? Are there ways to induce caramelization at lower temperatures than 65=B0C?
Thanks in advance and best wishes for you all, Michael Reiner
I am looking for the recipe for Dalton's Osmium Solution. I have a couple of references, but the details of the recipe are different. Does anyone have the citation of the original reference? Thank you very much!
Hello, This may seem a strange solution but why not use a smaller beam stop? All the beam stop is is a wire of Aluminum. You could make another beam stop of a lesser diameter. Try pulling some Aluminum wire. You can get a very small diameter, provided the alloy has enough ductility. I would suggest that it is not good idea to blast your CCD camera with the strong diffracted beam.
Good luck.
Alex Greene SCIENTIFIC INSTRUMENTATION SERVICES, INC. PMB-499, 1807 West Slaughter Lane, Number 200 Austin, Texas 78748-6200 Phone: 512/282-5507 FAX 512/280-0702
QUALITY ELECTRON MICROSCOPE REPAIR
-----Original Message----- } From: Erasmus, Willem (WJ) {willem.erasmus-at-sasol.com} To: 'microscopy-at-msa.microscopy.com' {microscopy-at-Sparc5.Microscopy.Com}
Could someone please point me to an on-line resource (or previous letter to this group) that provides a protocol for embedding early post-implantation (E8-9.5) mouse embryos in paraffin?
We have tried embedding in gelatin as for cryostating, but the wax does not infiltrate properly.
Many thanks,
-Ron Goldstein -- ----------------------------------------------------------------------------- Dr. Ron Goldstein email goldst-at-mail.biu.ac.il Dept. Life Sciences Tel. 972-3-531-8216 Bar-Ilan Univ FAX 972-3-535-1824 52900 Ramat-Gan, ISRAEL http://www.biu.ac.il/NAT/ls/goldstein.html -----------------------------------------------------------------------------
} Hi- } } I am looking for the recipe for Dalton's Osmium Solution. I have a couple } of references, but the details of the recipe are different. Does anyone } have the citation of the original reference? Thank you very much! }
Dalton, A.J. Anatomical Record 121:281, 1955
-- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
} } Subject: Re: Freeze fracture Need Help } } } } Dear freeze fracturers, } } } } We (BAL-TEC / Balzers) still manufacture double replica devices. Please } check our } } home page at bal-tec.com . For any questions please contact me off line. } } Best regards } } Alex Vogt } } BAL-TEC is a manufacturer of preparation equipment. } }
We would like to acquire STEM, SE, and BSE attachments for JEOL's JEM1200EX. Dealers welcome.
Marek Malecki, M.D., Ph.D. Director and Principal Investigator
Electron Microscopy Facilities and Department of Biology University of California at San Diego
address: 1500 Bonner Hall, University of California at San Diego 9500 Gilman Drive, La Jolla, CA 92093-0368 telephone - lab: 8585342484 telephone - office: 8588223373 pager: 8586161420 email: mmm-at-biomail.ucsd.edu
Dear list members! I am servicing the SEM Hitachi S-800 at the Moscow State University. It is time now to replace the field emission cathode in this microscope. But unlike the substitution of heating cathodes the substitution of field emission ones is rather composite procedure. Could anybody prompt anything about any FEGSEM in this occasion? I shall take your advises with gratitude. Please you could reply by E-mail: antron-at-space.ru, or by fax (in USA): 603-761-3208.
Victor Sidorenko, ANTRON Co. Ltd., scientific service, Moscow, Russia.
Well, I think you do not have to shield the camera from the direct beam. In fact, I recorded a lot of EDs on our CCD (a 679 1k x 1k from Gatan) of biological 2D crystals (Brink & Chiu, J. Struct. Biol. (1994) 113:23). About the only thing ocurring when recording ED like this is overflow of the charge from the pixels with incident irradiation to neighbouring pixels (spike formation). At really high dose per ED (more than 1 e/angstrom^2 on the specimen, equivalent to about 10^7 e in the central beam) you may get some remnant of this spike in subsequent EDs. This imprint result from charge that gets deposited into the bulk of the silicon. However, if you are recording ED from inorganic materials, more than likely you should not have these problems. Besides, the imprint can easily be removed by raising the CCD temperature from -30C to RT. Alternatively, if you are afraid you may damage the CCD without using a beam stop (although we recorded well over 10,000 on our SSC without any signs of damage) you could inquire with your EM manufacturer about a custom built beam stop. I'm afraid though you may find the cost of such a beam stop prohibitively high.
Good luck with your efforts.
Jaap
-- Jaap Brink, Ph.D. Biochemistry, One Baylor Plaza, Baylor College of Medicine, Rm. N420 Alkek Building, Houston, TX 77030 Phone: (713)798-6989 -- Fax: (713)796-9438 -- Email: jbrink-at-bcm.tmc.edu URL : http://ncmi.bioch.bcm.tmc.edu/~brink
On Mon, 6 Sep 1999, Erasmus, Willem (WJ) wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Dear Microscopists } } I have a problem obtaining diffraction patterns on our CCD camera. We have a } Philips CM200 TEM fitted with a Gatan Image Filter. Due to an extra } magnification factor introduced by the GIF the image on the TEM viewing } screen is much smaller than the image seen on the computer screen. Although } bothersome, this does not present too much of a problem in ordinary imaging. } } } However, when obtaining a diffraction pattern I find that one cannot shield } the CCD camera from the centre spot (transmitted beam) with the beam-stop } because the image of the beam-stop covers the diffraction pattern. The only } thing visible on the screen is the beam-stop. } } At the moment I am recording diffraction patterns on film, but is there a } way to use the CCD camera for this purpose ? } } }
The program is set for the meeting to be held at San Francisco State University on October 2nd. The program and abstracts are available at the meeting web page (http://online.sfsu.edu/~camicro/). Registration is also via this web page. Please join us.
greg antipa
Program Highlights below:
For program, flyers, and registration go to: http://online.sfsu.edu/~camicro/ or search camicro either at www.sfsu.edu or your favorite search engine
5th California Microscopy Colloquium The California State University & Northern California Society for Microscopy Saturday, October 2, 1999
Seven Hills Conference Center San Francisco State University
All fields of light and electron microscopy. Participants include scientists and students from academia and industry.
to include presentations by:
David Blake Life on Mars and life in the Mars Meteorite. Has the latter taken on a life of its own?
Jacek Gaertig Molecular and microscopic analysis of organelle assembly in Tetrahymena.
David Scharf Scanning Electron Microscopy in the 21st Century.
and 40 others
Register Online (http://online.sfsu.edu/~camicro/) Registration (From August 3rd to September 24th): Regular - $35, Student - $15 Registration includes both lunch and breaks Late Registration after September 24th & On Site: Regular - $35, Student - $15 For Late Registration, lunch may not be included dep. on the date of your registration.
or, in a pinch Greg Antipa Phone: (415) 338-2951 or EMail: antipa-at-sfsu.edu or FAX (415) 338-2295
Gregory A. Antipa Department of Biology San Francisco State University San Francisco CA 94132 Office/Lab (415) 338-2951 Email antipa-at-sfsu.edu FAX (415) 338-2295
The replacement of a Hitachi S-800 FE tip is fairly straightforward. A detailed procedure is in the service manual which you may or may not have.
This is not a trivial procedure but it is also not an overwhelming procedure: just tedious and not for someone who doe not have a "feel" for equipment. What I mean by a ""feel" for equipment' is someone who can work well with tools and instrumentation. If you have not don't try this at the lab.
Conversely, I would not be afraid of this either. A man put this together, a man can take it apart and repair it.
You will be working with an ultra-high vacuum system and consequently all the common sense procedures regarding cleanliness and attention to detail apply. Do not leave fingerprints, use several sets of lint-free gloves, rinse contaminated areas with reagent grade methanol, etc. I pre-clean the tools I use with acetone then methanol.
Make sure you have all the replacement parts including 6-inch conflat, new FE tip and springs.
As I can recall the procedure is as follows:
1. I usually ensure that I have all the replacement parts including a new FE tip, new springs, conflat gasket, new lint free gloves, aluminum foil, tweezers, 13mm socket with torque wrench. 2. I turn off all three the ion pump power supplies. 3. Remove the four piece shroud covering the ion pumps. Be careful not to short anything or turn the SEM power off then turn power back on to continue. 4. Turn off the " Display Power". 5. Remove the high voltage cable at the FE gun end. 6. Remove the three mu-metal shield from the column. 7. Clean and dust the FE surfaces. I use a dry paintbrush then follow the area using a lint-free cloth soaked in methanol. 8. Prepare a tabletop area to work on the gun. I dust the surface then follow with a lint-free cloth soaked in methanol. I then cover the area with aluminum foil. 9. Open the Ion-pump bypass valves (4) located to the right side of each of the ion pumps. CCW opens them. 10. Vent the specimen chamber. This will also vent the gun as the bypass valves are open. Venting with N2 helps. 11. Unbolt all 16 conflat bolts located at the top of the gun. Mark the gun chamber and gun with felt pen as a reference. 12. Refer to the operation manual: there are three silver sleeves and corresponding bolts in the toolkit. Looking at the gun studs from the top locate three studs that have the threads for the sleeve installation. remove the three studs and install the three sleeves and bolts. See manual for description as the manual has pictures that are difficult to descibe here. 13. CAREFULLY lift out the gun assembly. Lifting upward only. 14. Flip the gun assembly over and place on prepared table. 15. Use gloves at this point. Remove and discard old conflat. Replace with new, clean conflat opened from package. Cover open gun chamber with aluminum foil. 16. We are now working on the gun assembly. The FE tip has two pins:one insulated the other grounded to the assembly. Note the pin position. Loosen the two allen screw holding the pins. Remove the old FE tip. 17. The springs located on either side of the FE tip are now distorted and damaged. Remove the springs. Remove the pins located at the end of the springs. make sure the spring pins are not distorted. reshape them if necessary. Replace springs with the new ones ordered. When replacing the springs only screw on the springs with one turn at each end. In other words, do NOT screw the springs on as tight as they can. make the spring as straigjht as you can. 18. Re-install the FE tip observing the "polarity" - one side is insulated from the rest of the housing. Tighten the allen head screws after placing the FE tip in place. 19. Remove the aluminum foil from the gun chamber. 20. CAREFULLY re-install the gun ensuring that the pins align to the corresponding posts located down in the gun chamber. Not a easy task. 21. Using an ohm-meter, measure the resistance down in the gun assembly from the two side pins. These are the same two pins that you connect the "inner bake" cable to when baking the system.The resistance should be around 28 ohms. 22. If you have measured the proper resistance of 28 to 30 ohms, CONGRATULATIONS the worst is over. If not remove the gun and find out why. You may need to replace the pins again. 23. Remove the silver alignment bolt & sleeves. Tighten the gun bolts using a cross pattern and the proper torque listed in the instruction manual. Check resistance again. 24. Pump column down with bypass valves open. When pressure is better than 10-6, I bake the system (inner and outer) for at least 6 hours. 25. Close the three bypass valves along side of the ion pumps. The fourth bypass valve (bottom)is recommeded to be left open for some reason. 26. Follow the bake cycle procedure described in the instruction manual for a inner and out bake. 27. I usually bake the system three times before conditioning the gun for use. Gun conditioing is described in the instruction manual. 28. After the gun conditioning, the tip needs to be "blunted". Blunting is done by heavy flashing the tip three times in sucession. 29. The mu metal shields and the shrouds can be re-installed and the gun & column can be aligned. Gun & column alignment is also described in the instruction manual.
Easy wasn't it.
The gun replacement procedure should take 4 hours. Baking should take another three days for a total of about four days at best. This may seem like an overwhelming task but Hitachi has one of the best and easiest gun replacement procedures. Other designs require factory replacement and are the tip replacement cannot be done in the field.
Regards,
Earl Weltmer
Victor Sidorenko wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Dear list members! } I am servicing the SEM Hitachi S-800 at the Moscow State University. } It is time now to replace the field emission cathode in this } microscope. But unlike the substitution of heating cathodes the } substitution of field emission ones is rather composite procedure. } Could anybody prompt anything about any FEGSEM in this occasion? I } shall take your advises with gratitude. } Please you could reply by E-mail: antron-at-space.ru, } or by fax (in USA): 603-761-3208. } } Victor Sidorenko, ANTRON Co. Ltd., scientific service, Moscow, Russia.
I forgot to add the following step after step 24: activate the ion pumps.
Earl Weltmer wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } The replacement of a Hitachi S-800 FE tip is fairly straightforward. A } detailed procedure is } in the service manual which you may or may not have. } } This is not a trivial procedure but it is also not an overwhelming } procedure: just tedious and not for } someone who doe not have a "feel" for equipment. What I mean by a ""feel" } for equipment' is someone who } can work well with tools and instrumentation. If you have not don't try this } at the lab. } } Conversely, I would not be afraid of this either. A man put this together, a } man can take it apart and repair it. } } You will be working with an ultra-high vacuum system and consequently all } the common sense procedures } regarding cleanliness and attention to detail apply. Do not leave } fingerprints, use several sets of lint-free gloves, } rinse contaminated areas with reagent grade methanol, etc. I pre-clean the } tools I use with acetone then methanol. } } Make sure you have all the replacement parts including 6-inch conflat, new } FE tip and springs. } } As I can recall the procedure is as follows: } } 1. I usually ensure that I have all the replacement parts including a new FE } tip, new springs, conflat gasket, } new lint free gloves, aluminum foil, tweezers, 13mm socket with torque } wrench. } 2. I turn off all three the ion pump power supplies. } 3. Remove the four piece shroud covering the ion pumps. Be careful not to } short anything or turn the SEM power off then turn power back on to } continue. } 4. Turn off the " Display Power". } 5. Remove the high voltage cable at the FE gun end. } 6. Remove the three mu-metal shield from the column. } 7. Clean and dust the FE surfaces. I use a dry paintbrush then follow the } area using a lint-free cloth soaked in methanol. } 8. Prepare a tabletop area to work on the gun. I dust the surface then } follow with a lint-free cloth soaked in methanol. } I then cover the area with aluminum foil. } 9. Open the Ion-pump bypass valves (4) located to the right side of each of } the ion pumps. CCW opens them. } 10. Vent the specimen chamber. This will also vent the gun as the bypass } valves are open. Venting with N2 helps. } 11. Unbolt all 16 conflat bolts located at the top of the gun. Mark the gun } chamber and gun with felt pen as a reference. } 12. Refer to the operation manual: there are three silver sleeves and } corresponding bolts in the toolkit. } Looking at the gun studs from the top locate three studs that have the } threads for the sleeve installation. } remove the three studs and install the three sleeves and bolts. See } manual for description as the manual } has pictures that are difficult to descibe here. } 13. CAREFULLY lift out the gun assembly. Lifting upward only. } 14. Flip the gun assembly over and place on prepared table. } 15. Use gloves at this point. Remove and discard old conflat. Replace with } new, clean conflat opened from package. } Cover open gun chamber with aluminum foil. } 16. We are now working on the gun assembly. The FE tip has two pins:one } insulated the other grounded to the assembly. } Note the pin position. Loosen the two allen screw holding the pins. } Remove the old FE tip. } 17. The springs located on either side of the FE tip are now distorted and } damaged. Remove the springs. Remove the pins } located at the end of the springs. make sure the spring pins are not } distorted. reshape them if necessary. Replace } springs with the new ones ordered. When replacing the springs only } screw on the springs with one turn at each end. } In other words, do NOT screw the springs on as tight as they can. make } the spring as straigjht as you can. } 18. Re-install the FE tip observing the "polarity" - one side is insulated } from the rest of the housing. Tighten the allen head screws } after placing the FE tip in place. } 19. Remove the aluminum foil from the gun chamber. } 20. CAREFULLY re-install the gun ensuring that the pins align to the } corresponding posts located down in the gun chamber. } Not a easy task. } 21. Using an ohm-meter, measure the resistance down in the gun assembly from } the two side pins. These are the same two pins } that you connect the "inner bake" cable to when baking the system.The } resistance should be around 28 ohms. } 22. If you have measured the proper resistance of 28 to 30 ohms, } CONGRATULATIONS the worst is over. } If not remove the gun and find out why. You may need to replace the } pins again. } 23. Remove the silver alignment bolt & sleeves. Tighten the gun bolts using } a cross pattern and the proper torque listed in the } instruction manual. Check resistance again. } 24. Pump column down with bypass valves open. When pressure is better than } 10-6, I bake the system (inner and outer) for at least 6 hours. } 25. Close the three bypass valves along side of the ion pumps. The fourth } bypass valve (bottom)is recommeded to be left open for some reason. } 26. Follow the bake cycle procedure described in the instruction manual for } a inner and out bake. } 27. I usually bake the system three times before conditioning the gun for } use. Gun conditioing is described in the instruction manual. } 28. After the gun conditioning, the tip needs to be "blunted". Blunting is } done by heavy flashing the tip three times in sucession. } 29. The mu metal shields and the shrouds can be re-installed and the gun & } column can be aligned. Gun & column alignment } is also described in the instruction manual. } } Easy wasn't it. } } The gun replacement procedure should take 4 hours. Baking should take } another three days for a total of about four days at best. } This may seem like an overwhelming task but Hitachi has one of the best and } easiest gun replacement procedures. } Other designs require factory replacement and are the tip replacement cannot } be done in the field. } } Regards, } } Earl Weltmer } } Victor Sidorenko wrote: } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } Dear list members! } } I am servicing the SEM Hitachi S-800 at the Moscow State University. } } It is time now to replace the field emission cathode in this } } microscope. But unlike the substitution of heating cathodes the } } substitution of field emission ones is rather composite procedure. } } Could anybody prompt anything about any FEGSEM in this occasion? I } } shall take your advises with gratitude. } } Please you could reply by E-mail: antron-at-space.ru, } } or by fax (in USA): 603-761-3208. } } } } Victor Sidorenko, ANTRON Co. Ltd., scientific service, Moscow, Russia.
Yes, the danger of damaging the CAMERA itself is very low. I am not aware of any camera that is exposed to the beam directly. This would pose problems with sensitivity, damage to the CCD chip and many X-rays being recorded as well.
Cameras usually use some form of electron-light converter (Phosphor, YAG), then funnel the light to the CCD chip by use of a lens system or fiber-optic channel plate. Most CCD chips react to overexposure with light simply by "spilling" charge into the next pixels, which leads to "blooming". There are chips available that have anti-blooming guard rings around each pixel, but they are very expensive and they reduce sensitivity, as the guard ring area is not photo sensitive.
The part that suffers the most from exposure to high beam currents (as in the central diffraction spot) is probably the phosphor or YAG, which can be burned. Unlike during regular TEM, where the viewing screen is only used for viewing, a camera uses the same phosphor for viewing and recording images. You should try to avoid burning the phosphor to maintain good image quality later on.
Michael
Michael Bode, Ph.D. Soft Imaging System Corp. 1675 Carr St., #105N Lakewood, CO 80215 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- } From: Exchange Administrator Sent: Tuesday, September 07, 1999 12:08 AM To: Michael Bode
Well, I think you do not have to shield the camera from the direct beam. In fact, I recorded a lot of EDs on our CCD (a 679 1k x 1k from Gatan) of biological 2D crystals (Brink & Chiu, J. Struct. Biol. (1994) 113:23). About the only thing ocurring when recording ED like this is overflow of the charge from the pixels with incident irradiation to neighbouring pixels (spike formation). At really high dose per ED (more than 1 e/angstrom^2 on the specimen, equivalent to about 10^7 e in the central beam) you may get some remnant of this spike in subsequent EDs. This imprint result from charge that gets deposited into the bulk of the silicon. However, if you are recording ED from inorganic materials, more than likely you should not have these problems. Besides, the imprint can easily be removed by raising the CCD temperature from -30C to RT. Alternatively, if you are afraid you may damage the CCD without using a beam stop (although we recorded well over 10,000 on our SSC without any signs of damage) you could inquire with your EM manufacturer about a custom built beam stop. I'm afraid though you may find the cost of such a beam stop prohibitively high.
Good luck with your efforts.
Jaap
-- Jaap Brink, Ph.D. Biochemistry, One Baylor Plaza, Baylor College of Medicine, Rm. N420 Alkek Building, Houston, TX 77030 Phone: (713)798-6989 -- Fax: (713)796-9438 -- Email: jbrink-at-bcm.tmc.edu URL : http://ncmi.bioch.bcm.tmc.edu/~brink
On Mon, 6 Sep 1999, Erasmus, Willem (WJ) wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Dear Microscopists } } I have a problem obtaining diffraction patterns on our CCD camera. We have a } Philips CM200 TEM fitted with a Gatan Image Filter. Due to an extra } magnification factor introduced by the GIF the image on the TEM viewing } screen is much smaller than the image seen on the computer screen. Although } bothersome, this does not present too much of a problem in ordinary imaging. } } } However, when obtaining a diffraction pattern I find that one cannot shield } the CCD camera from the centre spot (transmitted beam) with the beam-stop } because the image of the beam-stop covers the diffraction pattern. The only } thing visible on the screen is the beam-stop. } } At the moment I am recording diffraction patterns on film, but is there a } way to use the CCD camera for this purpose ? } } }
re: DV-23 Vacuum Gauge Tube for a Gatan Ion Miller
Hi all, Obviously I was not thinking very clearly last week, several people=20 pointed out that Duniway Stockroom was the perfect place to go for=20 this part! Many thanks for the help.
Here is a summary of the response I got (not attributions)
1. Kurt Lesker has the DV-23 for $58. Duniway Stockroom has an equivalent for $56.
2. Have you tried Lesker (www.lesker.com)? They are in western PA but usua= lly ship stuff out quickly.
3. You should give Duniway a call. They had a booth at M&M
4. Teledyne Hastings Chicago office: (804) 723-3925 As of 1994!
5. Yeah--call Teledyne Hastings-Raydist and ask for the name of your local dealer. When I did this, they had a $75 minimum order (2 tubes), so I had a spare....
6. Try Duniway Stock Room www.duniway.com 800-446-8811. Their=20 replacement for the DV-23 is part DST-023
7. Teledyne Hastings - Tel:757-723-6531, Fax:757-723-3925, E-mail :=20 Charles fulmer-at-teledyne.com
Please note new FAX number.
John Mansfield PhD CPhys MInstP North Campus Electron Microbeam Analysis Laboratory 417 SRB, University of Michigan 2455 Hayward, Ann Arbor MI 48109-2143 Phone: (734) 936-3352 FAX (734) 763-2282 Cellular Phone: (734) 358-7555 Email: jfmjfm-at-engin.umich.edu URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html Location: Lat. 42=B0 16' 48" Long. 83=B0 43' 48"
We routinely record selected-area and parallel-nanopobe diffraction patterns with a bottom-mounted Gatan 1K x 1K CCD camera on our JEOL 2010F FEGTEM. Typical indicated camera lengths in the microscope are 15-20 cm to record spot or ring patterns for phase identification, and a bit less to get wide-angle patterns for orientation analysis from Kikuchi lines. The (12-bit) dynamic range of this camera is just about sufficient to capture the entire pattern intensity range up to the intense central beam with no beam stop. This usually takes a few adjustments of the exposure time so that the pattern center is just at or a little above saturation (at 14K to 16 K counts, depending whether you use dark current subtraction). To get the pattern intensity in range for recording times up to a few seconds, and of course to avoid damaging the YAG scintillator with the direct beam, you can reduce the intensity by choosing a small condenser aperture.
If you use dark current subtraction, be sure the beam deflection system of the microscope is set up to completely blank diffraction patterns. Otherwise, the automatically recorded dark current pattern will actually be a displaced diffraction pattern and the subtraction result will not be what you want. I usually use a continuous recording mode, and start recording with the camera blanked by the microscope viewing screen. In this mode, you can dynamically adjust the exposure time, set the contrast, and make other corrections before recording the final pattern.
Finally, lens hysteresis in the microscope can have a large effect on pattern (camera constant) calibration at these small camera lengths.
Larry
Larry Thomas Battelle, Pacific Northwest Laboratories Richland, WA
Larry.Thomas-at-pnl.gov (509) 372-0793
---------- From: Michael Bode Sent: Tuesday, September 7, 1999 6:50 AM To: 'Microscopy-at-MSA.Microscopy.Com' Subject: RE: Diff patterns
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Yes, the danger of damaging the CAMERA itself is very low. I am not aware of any camera that is exposed to the beam directly. This would pose problems with sensitivity, damage to the CCD chip and many X-rays being recorded as well.
Cameras usually use some form of electron-light converter (Phosphor, YAG), then funnel the light to the CCD chip by use of a lens system or fiber-optic channel plate. Most CCD chips react to overexposure with light simply by "spilling" charge into the next pixels, which leads to "blooming". There are chips available that have anti-blooming guard rings around each pixel, but they are very expensive and they reduce sensitivity, as the guard ring area is not photo sensitive.
The part that suffers the most from exposure to high beam currents (as in the central diffraction spot) is probably the phosphor or YAG, which can be burned. Unlike during regular TEM, where the viewing screen is only used for viewing, a camera uses the same phosphor for viewing and recording images. You should try to avoid burning the phosphor to maintain good image quality later on.
Michael
Michael Bode, Ph.D. Soft Imaging System Corp. 1675 Carr St., #105N Lakewood, CO 80215 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
-----Original Message----- } From: Exchange Administrator Sent: Tuesday, September 07, 1999 12:08 AM To: Michael Bode Subject: FW: Diff patterns
} ---------- } From: "brink-at-tiger.3dem.bioch.bcm.tmc.edu"-at-sparc5.microscopy.com[SMTP:"BRINK-at-T IGER.3DEM.BIOCH.BCM.TMC.EDU"-at-SPARC5.MICROSCOPY.COM] } Sent: Monday, September 06, 1999 8:24:38 PM } To: Erasmus, Willem (WJ) } Cc: 'microscopy-at-msa.microscopy.com' } Subject: Re: Diff patterns } Auto forwarded by a Rule } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html -----------------------------------------------------------------------.
Well, I think you do not have to shield the camera from the direct beam. In fact, I recorded a lot of EDs on our CCD (a 679 1k x 1k from Gatan) of biological 2D crystals (Brink & Chiu, J. Struct. Biol. (1994) 113:23). About the only thing ocurring when recording ED like this is overflow of the charge from the pixels with incident irradiation to neighbouring pixels (spike formation). At really high dose per ED (more than 1 e/angstrom^2 on the specimen, equivalent to about 10^7 e in the central beam) you may get some remnant of this spike in subsequent EDs. This imprint result from charge that gets deposited into the bulk of the silicon. However, if you are recording ED from inorganic materials, more than likely you should not have these problems. Besides, the imprint can easily be removed by raising the CCD temperature from -30C to RT. Alternatively, if you are afraid you may damage the CCD without using a beam stop (although we recorded well over 10,000 on our SSC without any signs of damage) you could inquire with your EM manufacturer about a custom built beam stop. I'm afraid though you may find the cost of such a beam stop prohibitively high.
Good luck with your efforts.
Jaap
-- Jaap Brink, Ph.D. Biochemistry, One Baylor Plaza, Baylor College of Medicine, Rm. N420 Alkek Building, Houston, TX 77030 Phone: (713)798-6989 -- Fax: (713)796-9438 -- Email: jbrink-at-bcm.tmc.edu URL : http://ncmi.bioch.bcm.tmc.edu/~brink
On Mon, 6 Sep 1999, Erasmus, Willem (WJ) wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Dear Microscopists } } I have a problem obtaining diffraction patterns on our CCD camera. We have a } Philips CM200 TEM fitted with a Gatan Image Filter. Due to an extra } magnification factor introduced by the GIF the image on the TEM viewing } screen is much smaller than the image seen on the computer screen. Although } bothersome, this does not present too much of a problem in ordinary imaging. } } } However, when obtaining a diffraction pattern I find that one cannot shield } the CCD camera from the centre spot (transmitted beam) with the beam-stop } because the image of the beam-stop covers the diffraction pattern. The only } thing visible on the screen is the beam-stop. } } At the moment I am recording diffraction patterns on film, but is there a } way to use the CCD camera for this purpose ? } } }
A few years ago I saw at an MSA meeting a poster detailing how a low vibration room for TEM's had been designed and constructed as part of a new building. It involved the pouring of a concrete slab uniquely for a TEM and separate from the walls to minimize vibration. I thought I had a copy of the information provided by the poster but cannot seem to find it. Does anyone know:
1) Who authored the poster and a contact number? 2) Where I might find MSA poster information archived? 3) Any other information related to building a low vibration room as part of a new building?
Thanks in advance for your time and help.
Sincerely,
Mick Thomas ------------------------- Mick Thomas UHV-STEM Laboratory E-1 Clark Hall Cornell University Ithaca, NY 14853
At 04:20 PM 9/7/99 -0700, Mick Thomas wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Hendrik O. Colijn colijn.1-at-osu.edu Campus Electron Optics Facility Ohio State University (614) 292-0674 http://web.ceof.ohio-state.edu Murphy's Law: "If anything can go wrong, it will." Commentary: "Murphy was an optimist."
A paper covering the installation concerns, including vibration, for a FEG-TEM can be found in the proceedings from the MRS Spring 1999 symposium "Electron Microscopy of Semiconducting Materials and ULSI Devices."
C.J.D. Hetherington, et.al., "Installing and Operating FEGTEM's," Mater. Res. Soc. Proc. Vol. 523, ed. by C. Hayzelden, C. Hetherington, and F. Ross, pp. 171-6.
Philip L. Flaitz IBM Analytical Services, Hopewell Junction, NY http://www.chips.ibm.com/services/asg Ph.......(914) 892-3094, FAX -2003 flaitz-at-us.ibm.com
Mick Thomas {mgt3-at-msc.cornell.edu} on 09/07/99 07:20:44 PM
To: microscopy-at-sparc5.microscopy.com cc:
Fellow microscopists,
A few years ago I saw at an MSA meeting a poster detailing how a low vibration room for TEM's had been designed and constructed as part of a new building. It involved the pouring of a concrete slab uniquely for a TEM and separate from the walls to minimize vibration. I thought I had a copy of the information provided by the poster but cannot seem to find it. Does anyone know:
1) Who authored the poster and a contact number? 2) Where I might find MSA poster information archived? 3) Any other information related to building a low vibration room as part of a new building?
Thanks in advance for your time and help.
Sincerely,
Mick Thomas ------------------------- Mick Thomas UHV-STEM Laboratory E-1 Clark Hall Cornell University Ithaca, NY 14853
Mick, The poster you saw was probably: "Design and implementation of a site for a one-Ångstrom TEM", John H. Turner, Michael A. O'Keefe and Robert Mueller, in 55th Ann. Proc. MSA, Cleveland, Ohio (1997) 1177-1178. Contact me or John turner if you want more details. -Mike O'Keefe p.s. see http://ncem.lbl.gov/frames/oam.htm for images obtained using the microscope in that room.
Mick Thomas {mgt3-at-msc.cornell.edu} wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} of the information provided by the poster but cannot seem to find it. Does } anyone know: } } 1) Who authored the poster and a contact number? } 2) Where I might find MSA poster information archived? } 3) Any other information related to building a low vibration room as part } of a new building? } } Thanks in advance for your time and help. } } Sincerely, } } Mick Thomas } ------------------------- } Mick Thomas } UHV-STEM Laboratory } E-1 Clark Hall } Cornell University } Ithaca, NY 14853 } } Phone: 607-255-0650 } Fax: 607-255-7658 } e-mail: mgt3-at-msc.cornell.edu } }
Mick Thomas wrote: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Fellow microscopists, } } A few years ago I saw at an MSA meeting a poster detailing how a low } vibration room for TEM's had been designed and constructed as part of a new } building. It involved the pouring of a concrete slab uniquely for a TEM } and separate from the walls to minimize vibration. I thought I had a copy } of the information provided by the poster but cannot seem to find it. Does } anyone know: } } 1) Who authored the poster and a contact number? } 2) Where I might find MSA poster information archived? } 3) Any other information related to building a low vibration room as part } of a new building? } } Thanks in advance for your time and help. } } Sincerely, } } Mick Thomas } ------------------------- } Mick Thomas } UHV-STEM Laboratory } E-1 Clark Hall } Cornell University } Ithaca, NY 14853 } } Phone: 607-255-0650 } Fax: 607-255-7658 } e-mail: mgt3-at-msc.cornell.edu
Mick,
Contact Black Engineering Company (shock, vibration and noise isolation): (800)747-2523 or (404)578-0999. It may or may not be the one on the poster, yet they do exactly what you are looking for. Cheers.
Does anyone on the list have any idea or experience in measuring the volume of fingerprints? Many Thanks in advance Dr. G.Sanders -------------------------------------------------------------------------------- -----------------------Dr. Giles SandersZeneca / SmithKline Beecham Centre for Analytical SciencesChemistry DepartmentImperial College of Science, Technology and MedicineLondonSW7 2AY
Dear William: It is so nice and comforting to see a gentleman who expresses concerns over such important matters. I wish I had you as an instructor. Thank you. Now to respond...I was pregnant with my first child while working as an EMst and left work in my 11th month. This was also the time when everyone was hopping on the bandwagon in AIDS research and I found myself working with blood serum etc. trying to image this virus. As a result, I gave birth to a healthy baby girl and experienced no problems. She is now a beautiful 15-year old. If all the precautions are taken: nitrile gloves, using an exhaust hood pulling at least 100 feet per sec., disposable lab coats, goggles, etc., there should be no problem. A whole unit ought to be devoted to these issues for all students when learning EM methods. Also if the scopes are monitored for X ray leakage and the meter shows only background readings, then don't worry. My only concern is when changing the filaments...maybe some residual x ray exposure. But I don't see any big problem. Just monitor this woman to make sure if she is using all the precautionary steps and she should be fine....and have fun!!!!
Regards, Maria
Maria Fazio-Zanakis Bioimaging and Molecular Histology Hoechst Marion Roussel, Inc. 1-908-231-3357 Fax: 1-908-231-3962 Email: Maria.Fazio-Zanakis-at-hmrag.com
What exactly do you mean by "volume of fingerprints"?
Is that the volume of the residue left by the finger? Is it the volume of the finger that left the imprint? Do you mean the area of the fingerprint?
I am by no means an expert on fingerprints, but unless you have some additional information, I would guess that the extraction of a volume from 2-dimensional data (such as a print) is very difficult. On the other hand, if you do have additional information that applies to all fingers of all people (perhaps something as an "internal finger pressure"), and you know the force that was used by the finger that left the imprint, you might be able to calculate the volume of the finger from the area of the fingerprint.
Michael
Michael Bode, Ph.D. Soft Imaging System Corp. 1675 Carr St., #105N Lakewood, CO 80215 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
} ---------- } From: Giles Sanders[SMTP:G.SANDERS-at-IC.AC.UK] } Sent: Wednesday, September 08, 1999 1:06:46 AM } To: microscopy-at-sparc5.microscopy.com } Subject: Fingerprints } Auto forwarded by a Rule } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Does anyone on the list have any idea or experience in measuring the volume of fingerprints? Many Thanks in advance Dr. G.Sanders ------------------------------------------------------------------------ -------- -----------------------Dr. Giles SandersZeneca / SmithKline Beecham Centre for Analytical SciencesChemistry DepartmentImperial College of Science, Technology and MedicineLondonSW7 2AY
We are looking for a conductive(minimize charging) epoxy to backfill cracks and voids in metals to minimize water, solvent and etchant leakage after polishing.
It is most distressing to attempt to do probe analysis on a leaking sample.
Michael: the solution is often simple depending on the level of accuracy = required. If one knows the density of fingerprint oil then one can = "weigh" several fingerprints and average out the volume. Also one could = acetone clean the finger free of natural oil and ink up the finger with = fingerprinting ink (which you could more easily determine the density of) = transfer to previously weighed gelatin paper and weigh a number of prints = (by subtraction) on a high quality analytical balance and determine the = volume since volume equals weight (actually mass) divided by density. = thanks
I am in need of updated information or websites on the transmission electron microscope and the scanning-tunneling microscope. Any help would be appreciated. Thank you.
Has anyone had success in performing LM autoradiography on GMA (JB4 gly= col methacrylate) sections? I'm having trouble keeping the emulsion on the=
sections during the wash step, after the slides have been fixed in photographic fixer. The area of emulsion overlying the sections only p= uckers and peels off within 3 minutes into the wash step. So far I've tried c= asting Kodak NTB2 undiluted or Ilford K.5 (dil=3D1:1) emulsions on clean slid= es with 4 um GMA sections, on gelatin subbed and unsubbed slides.
To develop autoradiograms I'm using:
D-19 developer (dil=3D1:1) ------4 minutes Stop bath (0.5% acetic acid in dist water)-----30 sec 30% sodium thiosulfate fixer (no hardener) -----5 minutes distilled water wash (slides vertical in vessel)----15 minutes
Next, I will be trying diluted Kodak NTB2 emulsion (dil=3D 1:1), elimin= ating the acid in the stop bath, and switching to a fixer with hardener added= (eg. Kodak fixer).. maybe a few other things, but I'm running short on time.=
Any suggestions or references would be greatly appreciated.
Thanks in advance :)
Figen Seiler Microscopist Abbott Laboratories Department of Microscopy & Microanalysis E-mail: figen.a.seiler-at-abbott.com
A soulution that works as well is to cut a hole in the floor and drive wooden piles in the hole. It only work on lower floors. but it is better a killing vibrations than concrete.
Gordon Couger 624 Cheyenne Stillwater, OK 74075 405 624 2855 GMT - 6:00 -----Original Message----- } From: Vitaly Feingold {vitalylazar-at-worldnet.att.net} To: Mick Thomas {mgt3-at-msc.cornell.edu} Cc: microscopy-at-Sparc5.Microscopy.Com {microscopy-at-Sparc5.Microscopy.Com}
A soulution that works as well is to cut a hole in the floor and drive wooden piles in the hole. It only work on lower floors. but it is better a killing vibrations than concrete.
Gordon Couger 624 Cheyenne Stillwater, OK 74075 405 624 2855 GMT - 6:00 -----Original Message----- } From: Vitaly Feingold {vitalylazar-at-worldnet.att.net} To: Mick Thomas {mgt3-at-msc.cornell.edu} Cc: microscopy-at-Sparc5.Microscopy.Com {microscopy-at-Sparc5.Microscopy.Com}
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This is a common problem when examining polished sections in the SEM. Due to shrinkage, there is often a gap between the edge of the sample and the plastic. This depends on the type of resin used and the speed of cure. Slower is better. The next important step is adequate ultrasonic cleaning in iso-propanol to remove all traces of the polishing compound. If the gap is very large, we infill with some more of the same epoxy and repeat the process, finally drying the solvent out with a hot air gun. Of course, we give a flash carbon coat and use carbon dag to give a good conducting path.
This seems to work for us, good luck!
Barry
} -----Original Message----- } From: Giles, Bill [SMTP:William.Giles-at-TIMET.com] } Sent: Wednesday, September 08, 1999 5:51 PM } To: Microscopy-at-Sparc5.Microscopy.Com } Subject: Conductive epoxy for SEM/microanalysis use? } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Greetings, } } We are looking for a conductive(minimize charging) epoxy to backfill } cracks } and voids in metals to minimize water, solvent and etchant leakage after } polishing. } } It is most distressing to attempt to do probe analysis on a leaking } sample. } } Someone out there must have a solution!! } } Any suggestions? } } Bill Giles } TIMET }
Kelvin Conference Centre=20 University of Glasgow Wednesday 10 November 1999=20
The format for this successful series of one day meetings remains largely u= nchanged, with a programme of talks=20 interspersed with time for posters and the Trade Exhibition.
The following topics will be presented by key-note speakers:
(a) Microscopy of the Underworld; Looking at the adhesive surface of cells by techniques including Interferen= ce reflection, TIRFM, Forster Energy transfer and Surface Plasmon Resonance Microscopy.=20 Prof. Adam Curtis, Institute of Biomedical and Life Sciences, University of= Glasgow=20
(b) Using electrons to explore materials. Prof. Alan Craven, Department of = Physics and Astronomy, University of Glasgow
(c) Egg Tapping; Biomineralisation studied by SEM, Acoustic Resonance and L= aser Scanning Microscopy. Prof. Sally Solomon, Veterinary Anatomy, University of Glasgow
(d) Digital Information Acquisition in the TEM. Dr Timon Fliervoet, Phillips Electron Optics Applications Laboratory, Eindh= oven =20 A special feature this year will be short, contributed, prize talks by youn= g "first time" speakers.
There will be sponsored Prizes and these enjoyable, interesting and useful = meetings provide an opportunity to meet and discuss ideas and tips with microscopists in all fields.
The Conference Centre provides interspersed Meeting, Exhibition, Poster, Bu= ffet and Bar facilities and there is ample car parking. The cost per head is =A320, including the buffet lunch, coffee and tea. Subsidised buses= will run from Aberdeen, Edinburgh and Glasgow (contact the local User Group). This one day meeting is CPD accredited.
Web site: http://www.abdn.ac.uk/~nhi691/smg99.htm has abstracts form=20 the following meetings: 1998 Dundee Meeting=20 1997 Dunblane Meeting=20 1996 Aberdeen Meeting=20 =20
---------------------- Kevin Mackenzie Electron Microscope unit Dept Zoology University of Aberdeen Tillydrone avenue Aberdeen AB24 2TZ ----------------- Tel 01224 272847 Fax 01224 272396 email k.s.mackenzie-at-abdn.ac.uk
I need to collect the general and special ideas and tips on how to prepare the multilayered thin film TEM specimens (Both plan view and cross sections), especially for the magnetic films.
Thank you very much for your help !
You can reply the message to: c.jiao-at-bham.ac.uk
Many EM immuno protocols using colloidal gold on grid mounted thin sections employ a 1% glutaraldehyde step after the grid is incubated in the secondary gold-conjugated antibody and washed. Does anybody know whether this step is really necessary (i.e., is there a paper that compares fix vs no fix after labeling). I would have thought the affinity of the antibody would have precluded the need for this step. Comments welcome.
Thomas E. Phillips, Ph.D. Associate Professor of Biological Sciences Director, Molecular Cytology Core Facility
3 Tucker Hall Division of Biological Sciences University of Missouri Columbia, MO 65211-7400 (573)-882-4712 (voice) (573)-882-0123 (fax)
Dear Bill, The last time I looked at buying conductive mounting epoxy, most suppliers listed a hot-press conductive resin, but not cold curing. I found a cold-curing, copper filled resin called Technovit 5000, from Germany. Kulzer: is the name of the company that makes it, but I ordered it from Energy Beam Sciences. It is a bit viscous to fill cracks, though. At 10:51 AM 9/8/99 -0600, you wrote: } } Greetings, } } We are looking for a conductive(minimize charging) epoxy to backfill cracks } and voids in metals to minimize water, solvent and etchant leakage after } polishing. } } It is most distressing to attempt to do probe analysis on a leaking sample. } } Someone out there must have a solution!! } } Any suggestions? } } Bill Giles } TIMET } Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
I have a student in my introductory electron microscopy course who has an implanted microprocessor controlled drug delivery pump. I am at the point where the "field trips" to the lab will start soon, and he asked me whether being close to the working microscopes (TEM at 75 kV, SEM at 5 kV) could interfere with his device. Although my initial thought was "I don't think so", I would like to make sure. One of the main things I've learned from reading the discussions that take place on this listserve is that there's a whole lot of physics out there that I don't know enough about.
I've posed this question to our environmental health and safety department (no answer yet), looked through some EM lab safety chapters in books, and checked the most excellent "Tips and Tricks of Microscopy" website to see if this has been discussed before. So far I haven't found any information.
I'd appreciate hearing from anyone who has insight into this kind of situation.
Thanks in advance,
Heather Owen
Heather A. Owen, Director Electron Microscope Laboratory Department of Biological Sciences University of Wisconsin - Milwaukee (414)229-6816
If we can answer any questions, give us a call at 800-440-0311.
Hope this is helpful.
Elinor Solit The Microscope Book
On Wed, 8 Sep 1999 Joelis99-at-aol.com-at-sparc5.microscopy.com wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I am in need of updated information or websites on the transmission electron } microscope and the scanning-tunneling microscope. Any help would be } appreciated. } Thank you. } } Joel Simons } joelis99-at-aol.com }
looks like you want to measure the volume of the actual fingerprint.
I could think of 4 ways to do that:
Use an AFM type of instrument. This may or may not work, depending on the characteristics of the fingerprint oil. It also may have a field of view that is too restricted. But if you can use such an instrument, it could give you the volume immediately.
Use a confocal microscope to get at the height data. If you can do that, it should be fairly easy to extract the volume. You may have problems if the fingerprint oil is transparent to the frequency used, or if the "height" of the fingerprint is below the resolution limit of the instrument.
Use a standard light microscope and take pictures at different focus settings. If the fingerprints have enough depth, you can reconstruct the surface from these images and get the volume that way.
Use a Stereo pair and calculate the 3rd dimension. This requires that the depth of the fingerprint is considerable. I would guess, that this does not work, but may be worth a try.
We have software to analyze all these measurements. Please contact me off-line if you want more info.
Thanks.
Michael Bode, Ph.D. Soft Imaging System Corp. 1675 Carr St., #105N Lakewood, CO 80215 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
} ---------- } From: Robert Mixon[SMTP:MIXONR-at-OHSU.EDU] } Sent: Wednesday, September 08, 1999 3:35:35 PM } To: Microscopy-at-sparc5.microscopy.com } Subject: RE: fingerprints } Auto forwarded by a Rule } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Michael: the solution is often simple depending on the level of accuracy required. If one knows the density of fingerprint oil then one can "weigh" several fingerprints and average out the volume. Also one could acetone clean the finger free of natural oil and ink up the finger with fingerprinting ink (which you could more easily determine the density of) transfer to previously weighed gelatin paper and weigh a number of prints (by subtraction) on a high quality analytical balance and determine the volume since volume equals weight (actually mass) divided by density. thanks
No great theory here but, by way of anecdotal input, this. In the past I've "borrowed" a TA for my TEM course from our MSM department. He has an insulin pump that, I believe, has a microprocessor control. He hangs around all sorts of TEM and SEM equipment for extend periods of time with no apparent (at least to my untrained eye) ill effects. He may even be lurking out there now and reply. cheers, John
John Heckman TEM Supervisor MSU Center for Electron Optics
Has anyone ever done in situ hybridization in cultured cells using 96-well culture plates? If so, do you have a favorite procedure that is followed? Any help would be greatly appreciated. Thanks in advance. Linda Chicoine Clinton, CT lchicoine-at-snet.net
I am soliciting contributors (or names of potential contributors) for a symposium for the Microscopy & Microanalysis meeting to be held on August 13-17, 2000 in Philadelphia, Pa.
Talks may range in length from 15 to 45 minutes.
A description of the symposium follows.
SYMPOSIUM: EXTREME ORGANISMS
This symposium will deal with organisms that represent extremes, as for example: the ability to grow in extreme environments, having extreme virulence or invasiveness, or being extremely difficult to visualize using conventional preparatory procedures. Hopefully, the participants shall describe some of the unique features of extreme organisms that give rise to these capabilities. Of particular interest shall be talks dealing with organisms able to grow in challenging environments such as high salt, high or low temperatures, high or low pressure and highly toxic conditions. In addition, speakers are invited to discuss organisms that are extremely virulent or invasive, as would be the case with certain pathogens. In this instance, whenever possible, speakers may identify the features of the organisms giving rise to this capability. Finally, extreme organisms are often difficult to visualize using standard preparatory procedures. Papers describing procedures to prepare the specimens for visualization would be germane to this symposium.
Please respond directly to me with the name of the individual and possible topic.
John Bozzola
#################################################################### John J. Bozzola, Ph.D., Director Micro-Imaging and Analysis Center 750 Communications Drive - MC 4402 Southern Illinois University Carbondale, IL 62901 U.S.A. Phone: 618-453-3730 Fax: 618-453-2665 Email: bozzola-at-siu.edu Web: http://www.siu.edu/departments/shops/cem.html ####################################################################
Just to clarify my origninal question, by volume of the fingerprint I meant the volume of material left by the fingerprint, not a volume of imprint. Thanks in adavnce and for those who have already replied. -------------------------------------------------------------------------------- -----------------------Dr. Giles SandersZeneca / SmithKline Beecham Centre for Analytical SciencesChemistry DepartmentImperial College of Science, Technology and MedicineLondonSW7 2AY
Recently I got very interesting results on PET/PMMA extrudate morphology(10 ~ 1 wt% of PMMA and PET was crystalline). Instrument: Hitachi S800 SEM Sampling method: hand fracturing or knife cutting under liquid nitrogen temperature. Results: Unexpectedly, hand fracturing sample has little matrix burrs while knife cutting sample has many. This is strange because it is usually think cutting is better way than fracture. Any suggestions? Thank inadvance for your help. Dr. Zeng JijunVisiting Scholar of Toray Home Address: Nakatogari 734-2-5 Nagaizumi-cho, Sunto-gun Shizuoka, 411-0942 Japan Tel: +81-559-884601(H)E-mail: sentoray-at-izu.co.jpPersonal Homepage:http://www.geocities.com/ResearchTriangle/Forum/1786
There are only five openings left for the "Cryoultramicrotomy and Immunolabelling Workshop" being held at Harvard Medical School, October 5-7, 1999. The deadline for registration has been extended until 9/13/99 for those needing hotel accommodations and until 9/28/99 for those who do not. For further information, please contact Sonja White (swhite-at-ebsciences.com) {mailto:swhite-at-ebsciences.com)} .
Best regards, Steven Slap .******************************************* Energy Beam Sciences, Inc. The Laboratory Microwave Company Adding Brilliance to Your Vision http://www.ebsciences.com {http://www.ebsciences.com} *******************************************
Hello everyone I have just started a new season of "Small Wonders" on Discovery Channel. This is a picture puzzle, usually taken on an SEM, with clues. You have to guess the answer and win a prize!
It is only shown on TV in Canada as it is on -at-Discovery Canada, every Monday evening 8-9pm (Pacific time). It is usually the last item on the show. If you are not in Canada, you can still participate in the competition from the website. If you want to see the show, you can download "RealPlayer" onto your computer.
-at-Discovery website: www.exn.ca/-at-discovery.ca Click on the Small Wonders icon down the right hand side
If you go to the Archives at the bottom of the Small Wonders page, you can see some of the images from last year. Challenge: see if you can tell what the picture is before you see the answer!
Last year was fun and I hope to have more fun this year. If you have any ideas, please let me know off line - we wouldn't want to give all the answers away would we?! Elaine
Dr. Elaine Humphrey Biosciences Electron Microscopy Facility University of British Columbia 6270 University Blvd, mail-stop Botany Vancouver, BC CANADA, V6T 1Z4 Phone: 604-822-3354 FAX: 604-822-6089 e-mail: ech-at-unixg.ubc.ca
Mary & Bill: Conductive silver epoxy in small twin tubes is available from ProSciTech and I expect some other "EM Suppliers". Its cold curing too. Cheers Jim Darley
ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com Great microscopy catalogue, 500 Links, MSDS, User Notes www.proscitech.com
On Friday, September 10, 1999 2:36 AM, Mary Mager [SMTP:mager-at-interchange.ubc.ca] wrote: } } Dear Bill, } The last time I looked at buying conductive mounting epoxy, most suppliers } listed a hot-press conductive resin, but not cold curing. I found a } cold-curing, copper filled resin called Technovit 5000, from Germany. } Kulzer: is the name of the company that makes it, but I ordered it from } Energy Beam Sciences. It is a bit viscous to fill cracks, though. } At 10:51 AM 9/8/99 -0600, you wrote: } } } } Greetings, } } } } We are looking for a conductive(minimize charging) epoxy to backfill cracks } } and voids in metals to minimize water, solvent and etchant leakage after } } polishing. } } } } It is most distressing to attempt to do probe analysis on a leaking sample. } } } } Someone out there must have a solution!! } } } } Any suggestions? } } } } Bill Giles } } TIMET } } } Regards, } Mary } } Mary Mager } Electron Microscopist } Metals and Materials Engineering } University of British Columbia } 6350 Stores Road } Vancouver, B.C. V6T 1Z4 } CANADA } tel: 604-822-5648 } e-mail: mager-at-interchg.ubc.ca }
I have an Eico sputter coater with a gold target. I used to coat my ceramic samples with the coater for several years, and did not have any problem. A couple of weeks ago, I tried to coat biological sample which was a part of an animal tongue The sample was dried thoroughly, accroding to the person who prepared the sample, but the other sample prep process he used was unknown, unfortunately. After the sputter coating, something went wrong, and I could not coat my ceramic samples well any more. I checked the gold target with an optical microscope, and I found that green particles or at least particle-like stuffs were inside the pits or grooves on the gold target. It seems that my ceramic samples were not coated with gold but with the green stuffs. And also, I found the surface of the gold target was very rough and the color was a little reddish, comparing with the other side of the target, but I was not sure if this resulted from the coating procedure of biological sample or from the long time wear. My question is how I can clean the gold target. Have any of you exprienced this kind of problem before? Any comment is appreciated.
Jondo Yun Electron Microscopy Laboratory Center for the Instrumental Analysis Kyungnam University Masan, Korea
I would dis-assemble, clean and rinse with acetone, for the parts that ca= n take acetone (non-plastics), then follow with an alcohol rinse. Changing the r= otary pump oil would not hurt either.
Earl Weltmer
=C0=B1=C1=B8=B5=B5 wrote:
} -----------------------------------------------------------------------= - } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Co= m } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.htm= l } -----------------------------------------------------------------------. } } Dear all } } I have an Eico sputter coater with a gold target. I used to coat my } ceramic samples with the coater for several years, and did not have any } problem. } A couple of weeks ago, I tried to coat biological sample which was a pa= rt of } an animal tongue The sample was dried thoroughly, accroding to the pers= on } who prepared the sample, but the other sample prep process he used was } unknown, unfortunately. After the sputter coating, something went wrong= , and } I could not coat my ceramic samples well any more. } I checked the gold target with an optical microscope, and I found that = green } particles or at least particle-like stuffs were inside the pits or groo= ves } on the gold target. It seems that my ceramic samples were not coated wi= th } gold but with the green stuffs. } And also, I found the surface of the gold target was very rough and the } color was a little reddish, comparing with the other side of the target= , but } I was not sure if this resulted from the coating procedure of biologica= l } sample or from the long time wear. } My question is how I can clean the gold target. Have any of you exprien= ced } this kind of problem before? } Any comment is appreciated. } } Jondo Yun } Electron Microscopy Laboratory } Center for the Instrumental Analysis } Kyungnam University } Masan, Korea } } jdyun-at-hanma.kyungnam.ac.kr
Dear Listers, I'm inquiring about the quality of the carbon coating one can obtain using the table top coaters equipped with a mechanical pump. Also, has anyone has upgraded such a system with a turbomolecular pump? Rosemary
My rough pumped (only) carbon yarn evaporator works quite well for SEM specimens. I have no experience using it for TEM specimens, but believe the turbo type is better suited for that application.
Woody White
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Dear Listers, I'm inquiring about the quality of the carbon coating one can obtain using the table top coaters equipped with a mechanical pump. Also, has anyone has upgraded such a system with a turbomolecular pump? Rosemary
We have tried both ways many times due to trouble shooting when the labeling doesn't work. For us it doesn't make any difference. If the primary stuck, the secondary seemed to stick and it didn't make any change in label frequency or efficiency whether it is fixed after the secondary or not.
Bob Morphology Core Seattle
On Thu, 9 Sep 1999, Tom Phillips wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Many EM immuno protocols using colloidal gold on grid mounted thin } sections employ a 1% glutaraldehyde step after the grid is incubated } in the secondary gold-conjugated antibody and washed. Does anybody } know whether this step is really necessary (i.e., is there a paper } that compares fix vs no fix after labeling). I would have thought } the affinity of the antibody would have precluded the need for this } step. Comments welcome. } } Thomas E. Phillips, Ph.D. } Associate Professor of Biological Sciences } Director, Molecular Cytology Core Facility } } 3 Tucker Hall } Division of Biological Sciences } University of Missouri } Columbia, MO 65211-7400 } (573)-882-4712 (voice) } (573)-882-0123 (fax) } }
Dear Gaetan, The S-570 cahmber is circular, with a flat front and is about 11.5 inches O.D. and 10 inches I.D. At 09:01 PM 9/9/99 -0500, you wrote:
} } Hi everybody, } Is there anybody who could give me the standard size of the chamber of the } Hitachi S-570? } } Thanks, } } Ga=EBtan } Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
Dear Jondo, I have wiped my gold target with acetone on a tissue to clean it. Sounds like something outgassed from the sample. Slimy biologists.;-) At 04:04 PM 9/10/99 +0900, you wrote:
} } Dear all } } I have an Eico sputter coater with a gold target. I used to coat my } ceramic samples with the coater for several years, and did not have any } problem. } A couple of weeks ago, I tried to coat biological sample which was a part of } an animal tongue The sample was dried thoroughly, accroding to the person } who prepared the sample, but the other sample prep process he used was } unknown, unfortunately. After the sputter coating, something went wrong, and } I could not coat my ceramic samples well any more. } I checked the gold target with an optical microscope, and I found that green } particles or at least particle-like stuffs were inside the pits or grooves } on the gold target. It seems that my ceramic samples were not coated with } gold but with the green stuffs. } And also, I found the surface of the gold target was very rough and the } color was a little reddish, comparing with the other side of the target, but } I was not sure if this resulted from the coating procedure of biological } sample or from the long time wear. } My question is how I can clean the gold target. Have any of you exprienced } this kind of problem before? } Any comment is appreciated. } } Jondo Yun } Electron Microscopy Laboratory } Center for the Instrumental Analysis } Kyungnam University } Masan, Korea } } jdyun-at-hanma.kyungnam.ac.kr } Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648 e-mail: mager-at-interchg.ubc.ca
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Reply to: RE: colloidal gold staining Dear Tom,
The glutaraldehyde step has been used for two primary purposes. The first = (and original) was to cross-link the gold probe to the primary antibody = and thus keep the signal in its correct location. However, binding of = antibodies or protein A to other antibodies is reasonably stable so many = protocols often omit this crosslinking step. The sections are often dried = soon after fixation which may also keep the gold in place. Other than the = original papers where the use of glutaraldehyde is described I don't know = of any papers comparing signal with and without this step.
The second use of 1% glutaraldehdye is when sections are being labeled with= two antibodies and two sizes of protein A gold. The usual protocol for = this in sequential labeling is: 1st antibody First gold probe (usually the smaller size) Blocking 2nd antibody Second gold probe.
To prevent the second gold probe from binding to the first antibody = earlier papers suggested the use of free protein A between the first gold = probe and the second antibody (the "Blocking" step). This effectively = covered any free protein A-binding sites that could confuse the double = labeling results. More recently, a 1% glutaraldehyde step has been used = to replace the free protein A-step. It seems to work better than the = protein A and is generally accepted to be a usable protocol for multiple = labeling. = = The reference for this is: van Genderen, I.L. van Meer G. Slot J.W. Geuze H.J. & Voorhout W.F. 1991. = Subcellular localization of Forssman glycolipid in epithelial MDCK cells = by immuno-electronmicroscopy after freeze-substitution. J. Cell Biol. 115:= 1009-1019. =
Regards,
Paul Webster, Ph.D House Ear Institute 2100 West Third Street Los Angeles, CA 90057 213 273 8026 213 413 6739 (fax) pwebster-at-hei.org http://www.hei.org/htm/aemi.htm
Tom Phillips wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America =
by attila.stevens-tech.edu (8.9.3/8.9.3/7) with ESMTP id NAA02448 for {Microscopy-at-sparc5.microscopy.com} ; Fri, 10 Sep 1999 13:53:50 -0400 (EDT) Message-ID: {37D9468D.B9519533-at-stevens-tech.edu}
Greetings,
Recently I have embedded biological polymer fibers in epoxy to section for TEM samples. Unfortunately, the fibers with very small diameters dissolved in the epoxy. The fibers are used for applications requiring the polymer to be absorbed in the human body. Therefore, they also dissolve in water. Does anyone have any suggestions for either alternate methods to obtain a TEM sample or ways to alleviate this problem?
I don't know where to start, so any advice would be very useful.
Thanks,
Jennifer Taylor Ph.D. candidate Stevens Institute of Technology Hoboken, New Jersey 07030
I have a former student who is working with hyaluronic acid microspheres. These microspheres are clearly visible under the stereomicroscope, however, the desire is to view them using SEM. The problem is that when they are applied to a stub and allowed to air dry they disrupt in some way leaving a film layer behind. They wish to visualize the intact microspheres. In addition, they might also want to try observing them using a TEM (having worked with embedding and sectioning spheres/bubbles of surfactant from the lungs I realize how difficult this might be). If they could be embedded and sectioned what stain(s) might be employed?
The following is a description of how these microspheres are prepared. Any assistance with even just a SEM protocol would be greatly appreciated!
HA Preparation: " A hyaluronic acid (HA)solution and an organic oil (with a small amount of emulsifying agent) are mixed thoroughly until an emulsion forms. ONce the emulsion forms, polymerizing and cross-linking chemicals are added to cross-link the HA. The mixture is then centrifuged and the oil layer is discarded. The HA microsphere pellet is then washed several times with isopropanol and resuspended in a minimum amount of distilled water and then lyophilized."
Regards,
Steve
Stephen J. Beck Associate Professor Bio-Imaging Center/Electron Microscopy Department of Biology Nassau Community College Garden City, NY 11530 Voice Mail: (516) 572-7829 Email: {becks-at-sunynassau.edu} URL: {http://www.sunynassau.edu/webpages/biology/becks.htm}
I have a question I have not been able to locate the answer to anywhere. I run a clinical EM lab here at the University of Nebraska and we have recently become associated with the College of Anatomic Pathologists (CAP) and I am having trouble locating any set regulations or even guidelines as to the duration we should retain records, as well as specimen blocks, thick section slides and EM photos. Does anyone have any info to get me headed in the right direction?
Thank you,
Doug Rennie Coodinator-EM Facility University of Nebraska Medical Center Omaha, Nebraska.
We are familiar with vacuum impregnation and the usual thermosetting products.
One of the problem areas that we have with using "normal" epoxy and carbon coating is that we primarily are trying to quantify nitrogen in titanium(Mary's ears perk up!) and our detection ability is so poor that we dont care to add the carbon layer.
Most EM grade silver epoxies seem to have a particle size of 10um, we are trying for something smaller. Master Bond sells a standard grade at 7um and a special grade at 3.5um also the epoxy is a NASA grade for low outgassing. I'm going to try some of the standard grade first and see how it performs.
Ill keep the list updated, if anyone else has other suggestions I'm all ears(well, not ALL).
I have been tasked with investigating feasibility of calcium ratioing for some of our researchers. Not knowing much about it, I wanted to see if anyone has any experience with systems doing this.
What manufacturers systems are recommended/not recommended? (Please reply privately if you feel you will offend manufacturers publicly).
Specifically, why do you recommend/not recommend that configuration?
What should I look for/look out for when asessing calcium ratioing systems? (Features, functionality, capabilities, etc.)
Suggest you talk to Ted Dixon, now with BPI in Waterloo, Ontario: 519-886-9013 aedixon-at-confocal.com Ted has worked for years on a confocal macro/microscope which images fingerprints on incredible surfaces... even the black plastic bags which, as he puts it, "seems to be the favorite wrap for murderers and criminals". He also does great physics and should be able to take you through the math to convert from the image to the measurement.
Let me know how it goes.
Best regards, Barbara Foster Consortium President Microscopy/Microscopy Education ...Educating microscopists for greater productivity.
125 Paridon Street Suite 102 Springfield, MA 01118 PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com Visit our web site {http://www.MME-Microscopy.com/education} ****************************************************** MME is America's first national consortium providing customized on-site workshops in all areas of microscopy, sample preparation, and image analysis.
At 02:06 AM 9/8/99 -0500, Giles Sanders wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
You should keep records, blocks, etc. on clinical material at least 10 years. So far, we have never thrown any out, but some is stored off campus. If you want help with CAP go to:
www.cap.org
On Fri, 10 Sep 1999 drennie-at-UNMC.EDU-at-sparc5.microscopy.com wrote:
} Date: Fri, 10 Sep 1999 13:34:37 -0500 } From: drennie-at-UNMC.EDU-at-sparc5.microscopy.com } To: microscopy-at-sparc5.microscopy.com } Subject: Regulations for records keeping in clinical work } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Good afternoon, } } I have a question I have not been able to locate the answer to anywhere. I run a clinical } EM lab here at the University of Nebraska and we have recently become associated with the } College of Anatomic Pathologists (CAP) and I am having trouble locating any set } regulations or even guidelines as to the duration we should retain records, as well as } specimen blocks, thick section slides and EM photos. Does anyone have any info to get me } headed in the right direction? } } Thank you, } } Doug Rennie } Coodinator-EM Facility } University of Nebraska Medical Center } Omaha, Nebraska. } } } }
Sara E. Miller, Ph. D. P. O. Box 3020 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-8735
The phenomenon won't be that strange at all if you take into consideration the following factors: 1. Fracture may include crack nucleation and expansion; 2. In LN, most polymers are brittle macroscopically, but may be ductile microscopically; 3. Loading effects on fracture mode (speed of loading, magnitude and distribution of stress). In your case, when you bend your sample, I would suspect that some pre-existed defects/cracks work as fracture nuclei and because of stress concentration in those locations (specifically at the sharp tips of the microcracks according to fracture mechanics), cracks expand much more easily and rapidly than the cutting case where the tip of the cut may actually be blunt microscopically depending on how good your Japanese knife is.
cy, PhD Rodel, The King of Polishing 451 Bellevue Rd Newark, DE 19713
On Thu, 9 Sep 1999, Zeng Jijun wrote:
} Date: Thu, 9 Sep 1999 21:02:22 -0500 } From: Zeng Jijun {sentoray-at-izu.co.jp} } To: Microscopy-at-sparc5.microscopy.com } Subject: SEM - sampling method of PET } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Recently I got very interesting results on PET/PMMA extrudate } morphology(10 ~ 1 wt% of PMMA and PET was crystalline). Instrument: } Hitachi S800 SEM Sampling method: hand fracturing or knife cutting under } liquid nitrogen temperature. Results: Unexpectedly, hand fracturing sample } has little matrix burrs while knife cutting sample has many. This is } strange because it is usually think cutting is better way than fracture. } Any suggestions? Thank inadvance for your help. Dr. Zeng JijunVisiting } Scholar of Toray Home Address: Nakatogari 734-2-5 Nagaizumi-cho, } Sunto-gun Shizuoka, 411-0942 Japan } Tel: } +81-559-884601(H)E-mail: sentoray-at-izu.co.jpPersonal } Homepage:http://www.geocities.com/ResearchTriangle/Forum/1786 } } } }
I archieved good labelling after stabilisizing my sections with 2% aqueous Glut. Without Glut, my signal was very weak. But collegues in my lab avoid this step, they say it caughts a lot of dirt or they see no diffence. So I would propose you should try it with Glut if your Immuno shows weak staining (with low background!) and you would like to enhance it.
Best wishes and good luck, Michael
Tom Phillips schrieb: } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Many EM immuno protocols using colloidal gold on grid mounted thin } sections employ a 1% glutaraldehyde step after the grid is incubated } in the secondary gold-conjugated antibody and washed. Does anybody } know whether this step is really necessary (i.e., is there a paper } that compares fix vs no fix after labeling). I would have thought } the affinity of the antibody would have precluded the need for this } step. Comments welcome. } } Thomas E. Phillips, Ph.D. } Associate Professor of Biological Sciences } Director, Molecular Cytology Core Facility } } 3 Tucker Hall } Division of Biological Sciences } University of Missouri } Columbia, MO 65211-7400 } (573)-882-4712 (voice) } (573)-882-0123 (fax)
Hi Folks, Does anyone have a feeling for the electric fields that a heated filiment sees as the "extraction field" in a typical TEM or SEM electron gun? Does this change for the Hexaboride filiments?
What is the effective emitting area of the cathode?
best regards mark
Mark W. Lund, PhD VP Engineering } } Soft X-ray Web page http://www.moxtek.com { { MOXTEK, Inc. 452 West 1260 North Orem UT 84057 801-225-0930 FAX 801-221-1121 lundm-at-xray.byu.edu
"This is a YOUNG business...How can I tell you what YOUR job is when I don't know what MINE is?" --Pogo
I have two rooms which I plan to convert into a confocal microscope suite during the makeover of our building. Our present experience is that the microscopes are sensitive to low frequency vibration caused by foot traffic in corridors and nearby stairs, closing of doors and movement of lifts. I want to minimize the effects of seismic and acoustic disturbance in the new room as near absolutely as is practicable, and proposed to the architects that we construct a vibration-isolated floor. This sounded like a brilliant idea until I was asked how exactly I would recommend them to do this. I have heard anecdotal accounts of a wide variety of solutions, many of which were outrageously expensive or totally impractical. Can anyone recommend the method that combines the *least* bangs per buck with proven efficacy?
Yours sincerely
Chris Jeffree ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Dr Chris Jeffree University of Edinburgh Biological Sciences EM Facility Daniel Rutherford Building King's Buildings EDINBURGH EH9 3JH Tel: +44 (0) 131 650 5345 FAX: +44 (0) 131 650 6563
The emitting area of a thermionic filament is typically significantly smaller for LaB6 than for Tungsten (I believe approximate numbers will be 5-15 microns for LaB6 and around 30 for tungsten hairpin). The area depends sensitively on Wehnelt bias.
Several textbooks have good sections treating this, for example the section In L. Reimer's textbook (Transmission Electron Microscopy, Springer-Verlag). In that text, there is a plot of calculated equipotential lines around a filament, with a reference to an article by Haine and Einstein (not Albert), J. Appl. Phys. vol. 3, (1952) p. 40
Regards, Wharton
------- } From: "Dr. Mark W. Lund" {lundm-at-physc3.byu.edu} } To: microscopy-at-sparc5.microscopy.com } Cc: lundm-at-physc3.byu.edu } Subject: Filiment fields } Date: Sat, Sep 11, 1999, 2:53 AM }
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} Does anyone have a feeling for the electric fields } that a heated filiment sees as the "extraction field" } in a typical TEM or SEM electron gun? Does this change } for the Hexaboride filiments?
Judging from the two assemblies for my JEOL SEM, i.e., a W assy and a LaB6 assy, the answer to your question is that the electrostatic fields are essentially the same, but that there are slight differences. That is, both my wehnelt assemblies are identical unless you get out the calipers and actually measure.
I made a program for you. It's a 32 bit Windows program. You can select as many LINK spectrum files (in their original, not converted format) as you need. All the selected files will be converted in one run. I have = tried it with more than 1700 spectra. The result of the conversion is a text = file with the same name but with different file extension. An example from a converted spectrum file (from a series): -------------------------------LAK11S103.SP------------------------------= ------ lak11s** 3
preset live time: 40 live time: 40 real time: 49 20.000000 eV/channel
Energy[keV], counts -0.200000, 0 -0.180000, 0 -0.160000, 0 -0.140000, 0 -0.120000, 3 -0.100000, 16 -0.080000, 42 -0.060000, 98 .. -------------------------------------------------------------------------= ------------ If you are still interested in a program like this, drop me a line and = I'll send it to you.
With the best regards: Laszlo Varga -----Eredeti =FCzenet----- Felad=F3: Paul Rennie (KIDDE) [SMTP:Paul.Rennie-at-kidde-hq.com] K=FCldve: 1999. augusztus 26. 17:29 C=EDmzett: Microscopy-at-Sparc5.Microscopy.Com T=E1rgy: EDX - Link AN10000 file conversion
------------------------------------------------------------------------ Dear list members,
We currently run a Link (now Oxford Instruments) AN10000 EDX system and = would like to convert all of our archived spectra into a format which can be read by a PC. [...] =20 Has anyone come across, or written a routine to perform this as a batch function?
Does anyone have a clever way of dealing with cryosections that stick to the knife? I can't dislodge them with a paintbrush or probe. The sections are stuck only along the cutting edge. Pulling them off with tweezers sometimes works, but more often than not they tear. The sections won't ribbon (which is okay, I prefer to get them one at a time) they just pile up. I tried touching the roll guard so that the section would stick to it, but that doesn't work. The sections are very nice otherwise.
Stephen J. Beck wrote: ========================================================= I have a former student who is working with hyaluronic acid microspheres. These microspheres are clearly visible under the stereomicroscope, however, the desire is to view them using SEM. The problem is that when they are applied to a stub and allowed to air dry they disrupt in some way leaving a film layer behind. They wish to visualize the intact microspheres. In addition, they might also want to try observing them using a TEM (having worked with embedding and sectioning spheres/bubbles of surfactant from the lungs I realize how difficult this might be). If they could be embedded and sectioned what stain(s) might be employed?
The following is a description of how these microspheres are prepared. Any assistance with even just a SEM protocol would be greatly appreciated!
HA Preparation: " A hyaluronic acid (HA)solution and an organic oil (with a small amount of emulsifying agent) are mixed thoroughly until an emulsion forms. ONce the emulsion forms, polymerizing and cross-linking chemicals are added to cross- link the HA. The mixture is then centrifuged and the oil layer is discarded. The HA microsphere pellet is then washed several times with isopropanol and resuspended in a minimum amount of distilled water and then lyophilized." ================================================ When a system of microspheres is vacuum sensitive, one approach we have taken over the years is to use our own SPI Supplies "Wet Replica" Kit (see our website below for details). It is a silicone based system that cures quickly, creating a "negative" replica of the vacuum sensitive microspheres. When cured, the silicone is then thoroughly rinsed with an appropriate solvent to remove all remains of the microspheres of interest. The microspheres are now represented as hollow spheres in the "negative".
Using another component from the kit, we then replicate the replica generating a "positive" replica from the negative. The positive is very stable, can be gold coated conventionally and examined by SEM.
There is one potential drawback of this method, and that is that above 700X, one starts to see structure from the replicating materials. However, for particle size measurements and structural observations below about 700X, this system should work just fine. We have not ourselves used it on HA, but on similar organic vacuum sensitive materials, including vacuum sensitive catalyst particles, it has worked just fine.
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President 1-800-2424-SPI SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
Look for us! ######################## WWW: http://www.spi.cc ######################## ============================================
} Date: Thu, 09 Sep 1999 09:09:03 +1200 } To: Microscopy-at-MSA.Com } From: Alfred Harris {a.harris-at-waikato.ac.nz} } Subject: SEM of Bacteriophage } } Hi everyone } I have had a request for electron microscope images of bacteriophage. I am familiar with standard TEM negative staining methods. Is it possible to obtain SEM images? Are they as good? What are best methods? } } Alfred Harris
"Fazio-Zanakis, Maria, HMR/US" wrote: } My only concern is when changing the } filaments...maybe some residual x ray exposure. But I don't see any big } problem.
There can't be any x-ray exposure as there is no x-ray output as there is no electron beam hitting anything to cause x-ray emission. This is self-evident (no e-beam) as you are changing the filament..
"Residual radiation" from x-ray tubes etc. with no HV and no filament heating is just an urban myth. It's impossible.
So, don't worry about changing filaments to your EM.
I have been trying to get decent plastic sections (3-5 um) of a succulent halophyte species, Salicornia virginica (perennial pickleweed). Preliminary work was done with paraffin embedding and sectioning. Results were good enough to get basic embryological sequence but really need better and thinner sections, especially for the very early prepollination stages. I have been using NaPO4 buffer pH 7.6, adjusted to match osmolality of tissue ( up to 1000 + msosm). Did inital fixation in 2.5 % glut + 2% paraformaldehyde + drop DMSO in .05 M buffer. Postfixed in 2% osmium ( also in buffer of same osmolality), then rinsed and dehydrated in graded EtOH. Finally embedded in LR White resin. All done on ice. Results are very poor. tissue appears badly shrunken and distorted, much of the cellular contents are gone. I have done the fixation without the DMSO and have also tried just fixing directly in osmium. It seems to me that the dehydration is what is causing the damage. I would appreciate any suggestions from the group. thanks, Mary Pfauth, Portland State University.
We have our own Ca-ratio imaging system with Fura-2. We developed the system in SCIL Image 1.4, a multipurpose image analysis platform. You can buy turnkey systems, but make sure they do it right:
The speed of the excitation filter canger is limiting for the speed of the application (besides equilibration of your Calcium-probe), if you use Fura-2. There are severla types of filterchangers possible.
If you also want morphological information and you want to use a camera, the videorate of a classical PAL camera is limited to 40ms time resolution (a bit faster for NTSC). A frame splitter gives you about 10 ms time resolution if it splits the frame in four. Alternatives (digital camera systems) can give you a better time resolution. Systems which only detect the luminance or faster camera-systems give you a better time resoloution.
You have to be careful to make the calculations right, as there are two pitfalls in the procedure:
1) Correct for the darkcurrent of your camera 2) Correct for the background noise, coming from the preparation.
Make sure that the detection system matches the dynamic range of the probe.
Sincerely yours,
Peter Van Osta, MD
Senior Scientist Medical Image Analysis Biological Imaging Laboratory Life Sciences Department I - 6065 Janssen Research Foundation Turnhoutseweg 30 B-2340 Beerse Belgium Europe
-----Original Message----- } From: "Mortro-at-aol.com"-at-sparc5.microscopy.com [mailto:"Mortro-at-aol.com"-at-sparc5.microscopy.com] Sent: Friday, September 10, 1999 10:56 PM To: microscopy-at-sparc5.microscopy.com
Hello all!
I have been tasked with investigating feasibility of calcium ratioing for some of our researchers. Not knowing much about it, I wanted to see if anyone has any experience with systems doing this.
What manufacturers systems are recommended/not recommended? (Please reply privately if you feel you will offend manufacturers publicly).
Specifically, why do you recommend/not recommend that configuration?
What should I look for/look out for when asessing calcium ratioing systems?
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has anybody positive or negative experience with the digital camera DC200 or DC100 for light microscopy sold by Leica? Before we bought DC200 we tested DC100 with lower resolution and found it useful. With DC200 we bought also a lot of problems. The main problems were that the programm under Win NT was not running properly and the white balance does not work. We always get a brown background. As we are investigating fungal infected plant tissues you can imagin that we cannot discriminate between health and necrotic tissue. .Now, we are waiting for month that Leica will solve the software problems with the white balance. It would be interesting for me, if anybody has got the same experiences with this system
Dr. Anne Heller Arbeitsgruppe Elektronenmikroskopie Institut fuer Botanik (210) Universitaet Hohenheim Garbenstr.30 D-70593 Stuttgart Tel.0049-711-459-2180 Fax 0049-711-459-3355
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Cryo-SEM should work fine for this sample. The freezing will stabilize the structure and careful sublimation should effectively remove enough ice to image the microspheres. You did not indicate the diameter of the microspheres but, providing they are not too small (probably not since they are readily seen with the stereomicroscope), they should be readily visible using cryo-SEM. We have done this with microspheres before with good success. For TEM, you may have the best luck with negative staining...again depending partially on size of the microspheres. The prep may take some experimentation....whether fixing will help stabilize the structure (as with using osmium to fix lipid microscpheres) and which negative stain works best. You may want to look at your sample prior to lyophilizing as this step could affect the size and shape of the microspheres.
Debby Sherman, Manager Phone: 765-494-6666 Microscopy Center in Agriculture FAX: 765-494-5896 Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu Purdue University 1057 Whistler Building West Lafayette, IN 47907-1057 --------------------------------------
Dear Colleagues,
I have a former student who is working with hyaluronic acid microspheres. These microspheres are clearly visible under the stereomicroscope, however, the desire is to view them using SEM. The problem is that when they are applied to a stub and allowed to air dry they disrupt in some way leaving a film layer behind. They wish to visualize the intact microspheres. In addition, they might also want to try observing them using a TEM (having worked with embedding and sectioning spheres/bubbles of surfactant from the lungs I realize how difficult this might be). If they could be embedded and sectioned what stain(s) might be employed?
The following is a description of how these microspheres are prepared. Any assistance with even just a SEM protocol would be greatly appreciated!
HA Preparation: " A hyaluronic acid (HA)solution and an organic oil (with a small amount of emulsifying agent) are mixed thoroughly until an emulsion forms. ONce the emulsion forms, polymerizing and cross-linking chemicals are added to cross-link the HA. The mixture is then centrifuged and the oil layer is discarded. The HA microsphere pellet is then washed several times with isopropanol and resuspended in a minimum amount of distilled water and then lyophilized."
Regards,
Steve
Stephen J. Beck Associate Professor Bio-Imaging Center/Electron Microscopy Department of Biology Nassau Community College Garden City, NY 11530 Voice Mail: (516) 572-7829 Email: {becks-at-sunynassau.edu} URL: {http://www.sunynassau.edu/webpages/biology/becks.htm}
RFC822 header -----------------------------------
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Alfred: I expect that FESEM could produce good images showing phages attached to bacteria. Conventional SEM does not have sufficient resolution. If, however, you need to show some structures within the phages or the shape of their heads, FESEM too is insufficient and negative staining/TEM with a final magnification in excess of 400k is required. Negative staining, to show bacteria and phages together at medium powers are rather difficult. Bacteria are too large for good negatively stained images, but you can be lucky. If no FESEM is available you could try your hand doing some metal shadow casting. Cheers Jim Darley ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com Great microscopy catalogue, 500 Links, MSDS, User Notes www.proscitech.com
On Monday, September 13, 1999 6:40 AM, Alfred Harris [SMTP:a.harris-at-waikato.ac.nz] wrote: } } } Date: Thu, 09 Sep 1999 09:09:03 +1200 } } To: Microscopy-at-MSA.Com } } From: Alfred Harris {a.harris-at-waikato.ac.nz} } } Subject: SEM of Bacteriophage } } } } Hi everyone } } I have had a request for electron microscope images of bacteriophage. I am } familiar with standard TEM negative staining methods. Is it possible to } obtain SEM images? Are they as good? What are best methods? } } } } Alfred Harris }
I am interested in receiving comments on the Soft Imaging System's AnalySIS Software including colorview 12 (LM) and Megaview II (TEM) cameras, specifically for biological applications. Please respond to me directly or to the list for comments of general interest. Commercial responses are welcome. Thanks.
Hello All, The LKB Ultrastainer 2168 in my lab is not functioning properly. The stain chamber is not filling completely and there is not enough stain flow through the tubing as well as water flowing through the rinse and wash cycles. The excellent service engineer for Leica is now working at a new company.
I would appreciate any help from anybody as to how to troubleshoot and fix this problem.
In a clinical EM setting, there is not much time allowed for malfunctioning instruments, especially if you work alone. Thanks, Winnie
At several colloidal gold workshops that I have attended, I was informed that the glut. fixation would also prevent the loss of signal during staining. Apparently, in some cases the rapid change in pH that occurs when moving a grid from a buffer or water rinse, into a uranyl acetate or lead citrate stain can cause the gold conjugates to break away from the antibodies. I must admit that I have never tried to stain without the glut. fixation so I don't know if there is any signal loss or not.
Tim Wakefield ----- / 101 Cary Hall / | \ / Auburn University, AL / --|-- \/ 36849 \ | /\ 334-844-3908 \ | / \ ----- \
I am in dire need of a camera for my Philips PSEM 500, but am starting to realize that this microscope is not exceptionally common. I understand that the same photomonitor module was used on the Philips STEM 400.
Does anyone out there have such a camera for sale?
Please? Pretty Please?
Paul Grover Microvista Laboratory Lafayette, Indiana USA
Additional comment on imaging microspheres which have been prepared by low temperature methods. Make sure you use the very lowest kV 2? if you must 3 and really low beam currents in the 10-20 pA range. If you have any ice in or around the sample, they are very beam sensitive.
Good luck
Patrick Echlin Cambridge UK On 13 Sep 1999, Debby Sherman wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Cryo-SEM should work fine for this sample. The freezing will stabilize } the structure and careful sublimation should effectively remove enough ice } to image the microspheres. You did not indicate the diameter of the } microspheres but, providing they are not too small (probably not since they are } readily seen with the stereomicroscope), they should be readily visible } using cryo-SEM. We have done this with microspheres before with good } success. } For TEM, you may have the best luck with negative staining...again } depending partially on size of the microspheres. The prep may take some } experimentation....whether fixing will help stabilize the structure (as with } using osmium to fix lipid microscpheres) and which negative stain works } best. } You may want to look at your sample prior to lyophilizing as this step } could affect the size and shape of the microspheres. } } Debby Sherman, Manager Phone: 765-494-6666 } Microscopy Center in Agriculture FAX: 765-494-5896 } Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu } Purdue University } 1057 Whistler Building } West Lafayette, IN 47907-1057 } -------------------------------------- } Date: 9/10/1999 1:28 PM } } From: Steve Beck } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Dear Colleagues, } } I have a former student who is working with hyaluronic acid microspheres. } These microspheres are clearly visible under the stereomicroscope, } however, } the desire is to view them using SEM. The problem is that when they are } applied to a stub and allowed to air dry they disrupt in some way leaving } a } film layer behind. They wish to visualize the intact microspheres. In } addition, they might also want to try observing them using a TEM (having } worked with embedding and sectioning spheres/bubbles of surfactant from } the } lungs I realize how difficult this might be). If they could be embedded } and } sectioned what stain(s) might be employed? } } The following is a description of how these microspheres are prepared. } Any } assistance with even just a SEM protocol would be greatly appreciated! } } HA Preparation: } " A hyaluronic acid (HA)solution and an organic oil (with a small amount } of } emulsifying agent) are mixed thoroughly until an emulsion forms. ONce the } emulsion forms, polymerizing and cross-linking chemicals are added to } cross-link the HA. The mixture is then centrifuged and the oil layer is } discarded. The HA microsphere pellet is then washed several times with } isopropanol and resuspended in a minimum amount of distilled water and } then } lyophilized." } } Regards, } } Steve } } } } } Stephen J. Beck } Associate Professor } Bio-Imaging Center/Electron Microscopy } Department of Biology } Nassau Community College } Garden City, NY 11530 } Voice Mail: (516) 572-7829 } Email: {becks-at-sunynassau.edu} } URL: {http://www.sunynassau.edu/webpages/biology/becks.htm} } } } } } } RFC822 header } ----------------------------------- } } Received: from SPARC5.MICROSCOPY.COM by mailcenter.btny.purdue.edu } with SMTP (QuickMail Pro Server for Mac 2.0r1b2); 10 SEP 99 } 18:47:57 UT } Received: (from daemon-at-localhost) by Sparc5.Microscopy.Com } (8.6.11/8.6.11) id NAA15527 for dist-Microscopy; Fri, 10 Sep 1999 13:14:57 -0500 } Received: from no_more_spam.com (Sparc5 [206.69.208.10]) by } Sparc5.Microscopy.Com (8.6.11/8.6.11) with SMTP id NAA15517 for } "MicroscopyFilteredEmail-at-msa.microscopy.com"; Fri, 10 Sep 1999 13:14:26 -0500 } Received: from lib.acs.sunynassau.edu (LIB.ACS.SUNYNASSAU.EDU } [198.38.8.2]) by Sparc5.Microscopy.Com (8.6.11/8.6.11) with SMTP id NAA15510 for } {Microscopy-at-MSA.Microscopy.Com} ; Fri, 10 Sep 1999 13:14:10 -0500 } Received: from nov1.acs.sunynassau.edu ([198.38.9.253]) } by lib.acs.sunynassau.edu with ESMTP } for Microscopy-at-MSA.Microscopy.Com; Fri, 10 Sep 1999 14:22:14 } -0400 } Received: from NCC_VOL2/SpoolDir by nov1.acs.sunynassau.edu (Mercury } 1.40); } 10 Sep 99 14:28:31 -500 } Received: from SpoolDir by NCC_VOL2 (Mercury 1.31); 10 Sep 99 14:28:29 } -500 } Received: from [198.38.8.22] by nov1.acs.sunynassau.edu (Mercury 1.31) } with ESMTP; } 10 Sep 99 14:28:24 -500 } X-Sender: becks-at-nov1.acs.sunynassau.edu } Message-Id: {l03010d03b3fefb765ba6-at-[198.38.8.22]} } Mime-Version: 1.0 } Content-Type: text/plain; charset="us-ascii" } Date: Fri, 10 Sep 1999 14:28:42 -0400 } To: Microscopy-at-Sparc5.Microscopy.Com } From: Steve Beck {becks-at-sunynassau.edu} } Subject: Microsphere Preparation } Errors-to: Microscopy-request-at-sparc5.microscopy.com } } } } }
Patrick Echlin Cambridge UKOn Mon, 13 Sep 1999, jean michel Wulveryck wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Dear microscopists, } I'm looking for the thermal conductivity of kraft. could someone give me } this feature. } Thanking You in advance, } Jean-Michel } } }
I am looking for a 3rd party to provide service and repairs to a used sem we will be purchasing in Nov. The prime SEM candidates are JEOL 6400FE or Hitachi 4000FE.
Bill Delaney Senior Fab Engineer Digital Optics Corp. Charlotte, NC bill-at-doc.com {mailto:bill-at-doc.com}
Jennifer Taylor wrote: ====================================== Recently I have embedded biological polymer fibers in epoxy to section for TEM samples. Unfortunately, the fibers with very small diameters dissolved in the epoxy. The fibers are used for applications requiring the polymer to be absorbed in the human body. Therefore, they also dissolve in water. Does anyone have any suggestions for either alternate methods to obtain a TEM sample or ways to alleviate this problem? ======================================= We have had this kind of problem with small polymer particles which tend to dissolve in any of the standard embedding systems. We solved this problem by taking the following approach:
• Take a flat clear embedding mold, for example, like our SPI 2442C-AB (see website address given below) and fill it half way with either SPI Pon™ 812 or one of the other popular "Epon® substitute" resins.
• After polymerizing into a block, apply sparingly some of the fibers, preferably in a dry form. It deposited from a liquid, surface tension forces interfere with being able to coat the underside of the fibers.
• Then metallize with Pt in a sputter coater. Au is OK if you don't have Pt, but Pt tends to be less likely to smear when thin sectioning.
• Then "disturb" (by shaking, or a slight blast with a duster) the fibers to the extent that at least some have been moved with the unmetallized side up and metallize again. We do this three times. The idea is to encapsulate the fibers in a passivation layer to provide protection from contact with the embedding resin.
• The cavity can then be filled up with resin and when cured, the block sectioned, resulting in undisturbed and umodified (by the resin) cross- sections for TEM. The resin should be put in in two steps, the first one being a very thin additional layer with the second layer deep enough to fill the cavity.
We would be very interested in doing a demo run for you using osmium instead of platinum since in theory at least, an osmium coating should be better than platinum (because of its amorphous nature and zero grain size). Let us know if you would be interested in our doing this.
Disclaimer: SPI Supplies manufactures and distributes some of the products mentioned so we would have a vested interest in their greater use. We have also been performing these kinds of laboratory analytical services since 1970.
Chuck
============================================
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President 1-800-2424-SPI SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
Look for us! ######################## WWW: http://www.spi.cc ######################## ============================================
------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Dear all,
I notice lots of calls for recommendations of software, CCD cameras, = diamond knives and other microscopy-related equipment appearing on this = list.
As I too am interested in comments about these products, and as I know = most replies occur off-line where I don't get to see them, wouldn't it be = great if there was a central site where we could submit our own reviews of = the equipment we use, and read other users comments?
At one of the on-line book sellers, the site allows for submission of book = reviews. When added together, the sum of these reviews gives a new reader = a good idea of what to expect. =
Couldn't we do this for our stuff too? In addition to giving the buyer an = informed view of expensive equipment, it might help suppliers identify = potential problems early enough to correct them.
Any comments, volunteers etc.?
Best regards,
Paul Webster, Ph.D. House Ear Institute 2100 West Third Street Los Angeles CA 90057 213 273 8026 http://www.hei.org/htm/aemi.htm
by williams.edu (PMDF V5.2-32 #39697) with SMTP id {0FI000GJZY90CZ-at-williams.edu} for microscopy-at-sparc5.microscopy.com; Mon, 13 Sep 1999 20:48:36 -0400 (EDT) Received: from localhost by colrain.williams.edu (5.65v4.0/1.1.8.2/16Jul96-0543PM) id AA13780; Mon, 13 Sep 1999 20:48:35 -0400
I've followed previous threads on printers, read published reviews, and spoken with the sales rep. Now I am seeking the opinions from users on the output, reliability, and service of four full-page, color, 300 dpi (or better) printers:
- Sony UP-D70A - Kodak 8660 or 8670PS - Fuji Pictography 3000
- also Codonics printers (sorry, no specific model)
We've been very pleased with the Epson 740 and 750 inkjet printers and glossy film, and now want to improve image quality and printing speed. We're running PCs on a LAN and can connect via parallel port, USB, Adaptec SCSI interface, or Ethernet. Our principal output will be high-resolution images embedded in desktop publishing software such as Word (and some Postscript programs too).
I am seeking opinions on a microspectrophotometer from SensorPhysics and Ocean Optics, called the Lambdascope. If you've used this system and are willing to share your opinions, please contact me off-list.
Hi all, A colleague has asked me to find out if anyone knows of any papers published on the use of environmental SEM to look at slurry. Any references or other information would be appreciated. Thanks.
Lyn Waterhouse CEMMSA Centre for Electron Microscopy and Microstructure Analysis University of Adelaide Adelaide SA 5005 Ph: (08) 8303 4074 Fax: (08) 8303 4356
Thanks to all the people who sent in suggestions; once again, this list has saved the day (or more accurately, the week).
Linda Barthel's ( http://www-personal.umich.edu/~praymond/protocol.html ) suggestion of cutting the block face into a diamond shape did the trick. And no compression at all !!! And you can forgo the roll guard if you're careful (though it worked for me with the roll guard as well).
In accumulating what I want I have come up with some extra Nikon, Zeiss, AO and Swift stuff that I would like to trade for things more useful to me. Everything is subject to return if is not what it is represented to be. I ask the same for what I trade for. I am looking for functional thing and cosmetics are not a sticking point. Optical quality is.
What I have:
A Zeiss Epi attachment for material work with an epiplan 40/.85 objective. It has bright field and dark field. It has a couple of filter slots and a diaphragm. It looks to be in great shape. This is the light tube that attaches to a stand and includes the turret. There is also an Attachment that allows it to attach directly to a binocular head.
A Zeiss condenser that probably went with the epi stuff it has a Epiplan .63 and 0.32 lens with two swing out filter trays that snap into place. The condenser has centering screws.
A focusing gear for the cream colored Zeiss. I wish I had got to it before some scraped it for metal:{
A black Nikon triocular head with a 1.2 relay lens and lighted pointer. The camera port is odd in that it comes off horizontally instead of vertically. It is for a TV camera and is not of standard eyepiece size. It has some dings in the paint but nothing that can't be detailed out. It says CirCon MV9585 micro optical system.
I have the Nikon binocular eyepiece head for it. It is a 95108 and has some chipping on the paint and needs cleaning. It has been forced from the retting ring and has a couple of ding on the curricular dove tail.These are not bad and can easily be filed out with no loss of functionality.
A Zeiss binocular eyepiece head is cream and folds in the center. It needs cleaning and the paint needs retouching here and there. There no dings or dents. Looking in the top at a good angle I can see some dirt or discoloration on the edges of one of the prisms where it is attached to the scope. This cannot be seen looking directly into the eyepieces and with eyepieces in the head. It appears to be normal to the mounting process.
A AO cycloptic that has a loose prism. It shows use. No stand or eyepieces. I would like to get the prism repaired and find a stand for this.
A 170 mm Leitz missing eyepieces and condenser but having a triouclar head. This is the 50 or 60 model that the course and fine focus is on the same knob. I really intend to keep this scope and most of what I am looking for is for it.
A Binkmann Medical scope that I believe was made by Zeiss in east Germany. Binkmann swears it is Zeiss and Zeiss says They never made it. It is a nice scope to use and is very nice condition. I am very happy with the scope. The only reason I mention it is that it might be of interest to a Zeiss collector if it is in fact made by Zeiss.
A 1 and 2 x head for a Swift binocular. It uses large Eyepieces and is missing eye pieces and stand. I would either like to find eyepieces and a stand or trade this. If I can find a stand I will machine adapters for standard eye pieces.
I have a Zeiss IKon 35mm w/focal plane shutter and front reflex housing and an attachment to eyepiece tube with photo eyepiece. I also have a single eyepiece tube. I know where there is a Second I can lay hands on if the deal is right. I are not very interested in trading these but would do it for the right stuff. Is in excellent shape
Now my want list. I am willing to trade most anything to end up with what I want.
I need some eyepieces; a pair of 8x, 15 x and a couple pairs of 10 x.
A good condenser for the Leitz. It is the one that rides on a Dovetail.
I would like a good phase setup for the Leitz and darkfeild would Be nice.
I need a stage micrometer. And I would be open to trade for some books on invertebrates and protozoa.
I need a stand for the Cycoplotic if I can get it fixed or I need a Stand for the Swift.
I would like to have a triocular head for the Binkman. It has A larger flange than the Zeiss. I can turn an adapter ring if necessary.
I am interested in adding functionality to the 170mm Leitz
A couple of mirrors.
A good light source.
I am currently in the San Francisco area for the next couple of weeks. I have some of the stuff with me. wold prefer to do business in person but I have done a great deal of internet business and have no problems with that.
My goal is to end up with two working binoculars and two working compounds with TV capability. Eyepiece cameras will be satisfactory For the binoculars. There are two of us working on this and most of My work under 100x will be done with a macro camera not a binocular Microscope. We have the video stuff already.
I am a ham radio operator and I have a good collection of stuff and access To a lot more. So if you want something in the electronics line I might have it.
Gordon Couger
408 249 7483 until the 27 of September. Call afternoon or evenings. Gordon Couger 624 Cheyenne Stillwater, OK 74075 405 624 2855 GMT - 6:00
I will setup Q & A forum about equipment at my Microscopy Vendors Database (http://www.kaker.com/mvd/vendors.html).
Henrik
Paul Webster wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } Dear all, } } I notice lots of calls for recommendations of software, CCD cameras, diamond knives and other microscopy-related equipment appearing on this list. } } As I too am interested in comments about these products, and as I know most replies occur off-line where I don't get to see them, wouldn't it be great if there was a central site where we could submit our own reviews of the equipment we use, and read other users comments? } } At one of the on-line book sellers, the site allows for submission of book reviews. When added together, the sum of these reviews gives a new reader a good idea of what to expect. } Couldn't we do this for our stuff too? In addition to giving the buyer an informed view of expensive equipment, it might help suppliers identify potential problems early enough to correct them. } } Any comments, volunteers etc.? } } Best regards, } } Paul Webster, Ph.D. } House Ear Institute } 2100 West Third Street } Los Angeles CA 90057 } 213 273 8026 } http://www.hei.org/htm/aemi.htm
} Chesapeake Society for Microscopy } presents } The Fall Dinner Meeting of 1999 } } Date: October 6th, 1999 } } Time: Social Hour at 6:00 PM } Graciously Sponsored by Ray Gundersdorff, JEOL } } Dinner: 7:00 PM--$20.00 (Students $10.00) } Menu includes several Chinese dishes } } Location: Far East Restaurant } } Speaker: Dennis Ward from the FBI } "Applications of Scanning Electron Microscopy at the FBI" } } } } Far East Restaurant } 5055 Nicholson Lane } Rockville, MD 20852 } (301) 881-5552 } From Beltway, North on 355 (Rockville Pike) } Make Right onto Nicholson Lane (Street right after Whiteflint Mall) } At 3rd light make left into parking lot } } Please make reservations by Oct. 5th } Contact Andrea Weisberg at (301) 435-1977 } Andrea S. Weisberg } NIH/NIAID/LVD } Bldg.4/Rm.210 } 4 Center Dr. } Bethesda,MD 20892-0445 } office (301) 435-1977 } Fax (301) 480-1147 } e-mail: aweisberg-at-nih.gov } }
ATHENE DONALD (Cambridge University) is one of our UK experts on ESEM - perhaps these three articles might be helpful:
(2) TI: The study of water in heterogeneous media using environmental scanning electron microscopy AU: Thiel_BL, Donald_AM JN: JOURNAL OF MOLECULAR LIQUIDS, 1999, Vol.80, No.2-3, pp.207-230
(14) TI: Direct observation of water-oil emulsion systems in the liquid state by environmental scanning electron microscopy AU: Stokes_DJ, Thiel_BL, Donald_AM JN: LANGMUIR, 1998, Vol.14, No.16, pp.4402-4408
(16) TI: Environmental scanning electron microscopy for the study of 'wet' systems AU: Donald_AM JN: CURRENT OPINION IN COLLOID & INTERFACE SCIENCE, 1998, Vol.3, No.2, pp.143-147
On Tue, 14 Sep 1999, Lyn Waterhouse wrote:
} A colleague has asked me to find out if anyone knows of any papers } published on the use of environmental SEM to look at slurry. Any } references or other information would be appreciated. Thanks.
} Lyn Waterhouse
+------------------------------------------------------------------------+ | Robert H.Olley Phone: | | J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 | | University of Reading {University internal extension 7867 | | Whiteknights Fax +44 (0) 118 9750203 | | Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk | | England URL: http://www.reading.ac.uk/~spsolley | +------------------------------------------------------------------------+
First of all the application framework I will talk about is NOT a commercial application, it is our own in-house developped digital image analysis framework for analysing microscopical images. I want to know if there are any commercial equivalents on the market to what we have here ?
We have developed (already a copule of years ago) a multi-mode multi-position automated image analysis system for micrscopy. The system is based on SCIL Image 1.x (http://carol.wins.uva.nl/~koelma/isis/projects/scilimage.html), an image analysis framwork from the University of Amsterdam in the Netherlands.
In one setup for fluorescence micrscopy, we are capable of acquiring 28380 grey-scale image in less than 4 hours from the central 60 wells of a 90 well plate (473 images per well), including focussing every 4 positions in the well. We use a ZEISS Axiovert 135, with a 40x objective, a motorised stage and an intensified camera to acquire the images. Our (patented) focusing algorithm is capable to focus even if the S/N ratio is 5 dB, ie. low quality fluorescence images.
The position recording system allows us to scan the plates in several modes, ie. different fluorochromes or different imaging modes (brightfield, phase contrast, DIC,...).
Analysis of the 28380 images takes about 9 hours, depending a bit on the application. The acquisition (SGI O2) and the analysis (SGI Origin200) is done on Silicon Graphics workstations.
Are there any commercial systems that are capable of doing this ? Our application is NOT for sale, so this is not a commercail spam.
Sincerely yours,
Peter Van Osta, MD
Senior Scientist Medical Image Analysis Biological Imaging Laboratory Life Sciences Department I - 6065 Janssen Research Foundation Turnhoutseweg 30 B-2340 Beerse Belgium Europe
Hey, a "Comsumer Reports" of microscopy equipment. Great idea. Are you volunteering to catalog them???? Maybe it's a project MSA would support with a small grant to keep the data base????
On 13 Sep 1999, Paul Webster wrote:
} Date: 13 Sep 99 17:44:04 -0700 } From: Paul Webster {pwebster-at-mailhouse.hei.org} } To: MSA listserver submission {Microscopy-at-sparc5.microscopy.com} } Subject: Recommnedations } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Dear all, } } I notice lots of calls for recommendations of software, CCD cameras, diamond knives and other microscopy-related equipment appearing on this list. } } As I too am interested in comments about these products, and as I know most replies occur off-line where I don't get to see them, wouldn't it be great if there was a central site where we could submit our own reviews of the equipment we use, and read other users comments? } } At one of the on-line book sellers, the site allows for submission of book reviews. When added together, the sum of these reviews gives a new reader a good idea of what to expect. } Couldn't we do this for our stuff too? In addition to giving the buyer an informed view of expensive equipment, it might help suppliers identify potential problems early enough to correct them. } } Any comments, volunteers etc.? } } Best regards, } } Paul Webster, Ph.D. } House Ear Institute } 2100 West Third Street } Los Angeles CA 90057 } 213 273 8026 } http://www.hei.org/htm/aemi.htm } } } }
Sara E. Miller, Ph. D. P. O. Box 3020 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-8735
What you have provided is the URL for Scion Image which was developed from NIH image. That is good to know, too.
However, Image Tool is a different product from the University of Texas Health Sciences Center. It is true that the links below do not work. Unfortunately, they are the ones listed officially on all of the UTHSCA pages that I found. I finally called Dr. Dove, who was involved in developing the program, and he directed me to the correct site. It is available through the main UTHSCA page following a "Resources" link, and finding the "Image Tool" listing. The end URL is http://macorb.uthscsa.edu/dig/itdesc.html
The demand was outstripping their old server and they had to move the site. Also, there is apparently more demand for improving and updating the program itself than there is for updating the web pages.
At 06:50 PM 9/3/1999 -0700, you wrote: } } I think UTHSCA ImageTool was taken over by Scion Image(???) At any rate, } Scion has an easy to use image analysis program that can be downloaded for } free from their site: } } http://scioncorp.com } } Sometimes I have LUT problems, but not when I'm doing image analysis. } (Before I got Photoshop, I used to use it to draw pictures ;-) ) } } ------------------------------------- } } On Fri, 3 Sep 1999, Andrew Ochalski wrote: } } } } } Dear Microscopists, } } } } I have been trying in vain for the past two } } weeks to download the most recent version of } } UTHSCSA's ImageTool program. The two links } } I have been trying from a variety of approaches } } are: } } http://ddsdx.uthscsa.edu } } and } } ftp://maxrad6.uthscsa.edu. } } Are these still valid, are there more current links } } or is the software no longer available ? Thanks } .. }
Hi all - taking a slurry as any mixture of solid particles in liquid? we have looked at a few fibre/oil mixes, and found the influence of kV to be crucial. 25kV imaged particles through the surface of the liquid without giving any detail of the liquid, at 10kV the liquid/gas boundary was more apparent, but at 5kV there was quite a dramatic shift, as detail on the surface of the liquid phase became visible (mixed SE/BSE signal) - what looked like a perfectly smooth surface even at 10-15kV was actually covered in gunk or had its own microstructure. You need (IMHO) helium (around 1 torr. give or take a bit) in the chamber to work comfortably at 5kV. Lower kV than 5 is probably practical on the newer model VP or ESEMS.
But if its a pure water slurry you are looking at, maybe it would be possible to drop the temperature as much as you can, and to mix in something else, to cut the evaporation rate to let you work at lower pressure to let you work at lower kV....?
cheers
Sally
Dr Sally Stowe Facility Coordinator Australian National University EM Unit Research School of Biological Sciences Box 475, ACT 2601, Canberra, Australia FAX 06 (0)2 6279 8525 http://www.anu.edu.au/EMU/home.htm
} } } Lyn Waterhouse wrote:
Hi all, A colleague has asked me to find out if anyone knows of any papers published on the use of environmental SEM to look at slurry. Any references or other information would be appreciated. Thanks.
Lyn Waterhouse CEMMSA Centre for Electron Microscopy and Microstructure Analysis University of Adelaide Adelaide SA 5005 Ph: (08) 8303 4074 Fax: (08) 8303 4356
I use a Kodak DS8650PS dye sublimation printer with Kodak ExtraLife XL paper and ribbon, 8-1/2"x12" paper. It is connected to a 10BaseT LAN with Macs and PCs (Win95) and HP Laserjets.
The quality of the Kodak is very good. However, each page costs about $2, between the cost of the paper and the ribbon. Printing speed is rather slow, even with 64MB of RAM. Sending anything higher than 300dpi is a waste. 220 dpi works well and does speed things up. The printer is a CMYK + coating so it makes 5 passes. Each print takes about 3-4 minutes to print. But it can take up to 5 minutes to just get the data file into the printer. So from hitting the Print command in a program, you can expect to wait at least 10 minutes for a printed page to emerge.
The ExtraLife is very good stuff since it is for all practical purposes, indestructible and does not fade with age. You can soak it in water and just wipe off and there is no damage to the print.
I have had sporadic luck doing direct printer port outputs to the 8650PS. But the Ethernet link never fails from the PC or Mac.
These printers can be found used for between $2,000 and $4,000. The newer models are supposedly faster but I'd suspect the difference is not all that much. But I have not seen one in action to verify that.
gary g.
At 05:48 PM 9/13/99 , you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I have the space to keep the data base. I don't have the expertise to edit it. It is a pretty good link. It is not as fast as the best but the congestion is low enough it makes up for it most of the time.
I will consider any form of internet publication. I am looking for a small circulation juried journal. I am primarily interested in increasing the speed of information distribution and reducing the cost.
My experience with some of my own publications where it took a year to get it published after acceptance and $700 bucks for reprints and they kept the copyright left a bad taste in my mouth.
If we are going to increase the rate of progress in technology we need to get the time to publication down as low as possible. I think it should be possible to get a non controversial jurried article out in two months and letters out in hours.
I am interested in publishing only. I am no editor. All copyrights stay with the author and no page cost at this point. The cost of publication on the internet is very low. My cost for keeping a box on the net are 50 a month and hardware replacement. $1,000 a year covers the cost including administration after the first time set up. So very modest page charges would be very attractive to and ISP. My interest is getting it started.
My cost will go up with a lot of net acess. But not much.
Gordon Gordon Couger 624 Cheyenne Stillwater, OK 74075 405 624 2855 GMT - 6:00 -----Original Message----- } From: Sara Miller {saram-at-duke.edu} To: Paul Webster {pwebster-at-mailhouse.hei.org} Cc: MSA listserver submission {Microscopy-at-Sparc5.Microscopy.Com}
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We are considering revamping our billing software in a multi-user, multi-instrument imaging facility. Can anyone suggest a good software package that they currently use for billing?
Thanks in advance for any suggestions.
Doug Taatjes
Dr. Douglas J. Taatjes Department of Pathology Director, Cell Imaging Facility University of Vermont Burlington, VT 05405 USA 802-656-0373 (voice) 802-656-8892 (FAX)
Web Page: http://pathology.uvm.edu/cifweb/cif_home/cif_index.html
Glutaraldehyde fixation after immunogold staining prevents the loss of signal that may be induced by low pH contrasting, such as in Uranyl salt solutions. Next to this Uranyl salts may have chaotropic effects. Both chemical characteristics are exploited in eluting antibodies form antigens in affinity chromatography purification techniques, as well as in eluting immunoglobulins from Protein A or Protein G affinity columns. In our experience the effect of the fixation step is more obvious with protein A or G reagents than with secondary antibody gold conjugates, where it will depend on Kd-values for the individual reactions beteen primary and secondary antibody. In fact Protein A gold may even become uncoupled from the primary antibody simply by washing in water. Of course it is necessary to rinse well after the gold incubation step before the on-set of fixation, since all adhering gold conjugates may become fixed to the specimen, both the ones bound to the primary as the unbound ones which should first be removed by washing. The fixation time can be relatively short, in the order of minutes, for on-section labeling and may have to be applied for a longer period of time for pre-embedding applications.
Hope this helps to clarify this =========================== Jan Leunissen AURION http://www.aurion.nl Costerweg 5 6702 AA Wageningen The Netherlands phone: 31-317-497676 fax: 31-317-415955 You will find more technical info on our web site
I notice lots of calls for recommendations of software, CCD cameras, diamond knives and other microscopy-related equipment appearing on this list.
As I too am interested in comments about these products, and as I know most replies occur off-line where I don't get to see them, wouldn't it be great if there was a central site where we could submit our own reviews of the equipment we use, and read other users comments?
At one of the on-line book sellers, the site allows for submission of book reviews. When added together, the sum of these reviews gives a new reader a good idea of what to expect. Couldn't we do this for our stuff too? In addition to giving the buyer an informed view of expensive equipment, it might help suppliers identify potential problems early enough to correct them.
A super idea! The problem for me as for anyone else who volunteers will be finding the time but it will be well spent. Count me as a volunteer! Rosemary
Our Fuji Pictrography has developed a problem where the donor paper gets jammed almost every cycle (often at 1/2 but also at further along the pathway). Have any Fuji owners had this problem and solved it? Any help would be gratefully appreciated.
Someone asked recently about high end printers including the Fuji. We love ours but the St. Louis Service rep that is responsible for our territory is terrible and is hard to even get to them to return a call. Fuji doesn't have a help line, they refer us to our rep. So despite having a great printer, their technical help suffers.
Thanks, Tom
Thomas E. Phillips, Ph.D. Associate Professor of Biological Sciences Director, Molecular Cytology Core Facility
3 Tucker Hall Division of Biological Sciences University of Missouri Columbia, MO 65211-7400 (573)-882-4712 (voice) (573)-882-0123 (fax)
I totally agree with this, Journal review is taking far too long now. Scientists should really put more effort in reviewing their co-workers paper submissions. Papers sitting on someone's desk for six months without review is unacceptable. I personally try to finish a review once I receive it within two weeks. Many people asked to review papers often never reply to the publishers, which I think is a serious offence in the scientific field.
I know the NIH is considering doing rapid article publication on their website. After screening of the articles, they are placed onto the web for anyone to download or read. I think there are other journals going this route as well. It is the communication method of the future. I think that publications will go this way. More respected online journals will have extensive peer review and screening. Other rapid publication journals will have less, and still be immensely useful in being able to do online searching for specific information.
I have a lot of experience in programming, web design, and system administration. I am interested in forming a rapid publication web site for the scientific community and will readily offer my skills and time. Gordon Vrdoljak.
\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\ Gordon Ante Vrdoljak 1 Cyclotron Road ICQ 23243541 http://nature.berkeley.edu/~gvrdolja MS90-1116 GAVrdoljak-at-lbl.gov Ernest Orlando phone (510) 495-2829 Lawrence Berkeley fax (510) 486-7797 National Laboratory cell (510) 290-6793 Berkeley CA 94720
On Tue, 14 Sep 1999, Gordon Couger wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I have the space to keep the data base. I don't have the expertise } to edit it. It is a pretty good link. It is not as fast as the best but } the congestion is low enough it makes up for it most of the time. } } I will consider any form of internet publication. I am looking for } a small circulation juried journal. I am primarily interested in } increasing the speed of information distribution and reducing } the cost. } } My experience with some of my own publications where it took } a year to get it published after acceptance and $700 bucks for } reprints and they kept the copyright left a bad taste in my mouth. } } If we are going to increase the rate of progress in technology we } need to get the time to publication down as low as possible. I think } it should be possible to get a non controversial jurried article out } in two months and letters out in hours. } } I am interested in publishing only. I am no editor. All copyrights } stay with the author and no page cost at this point. The cost of } publication on the internet is very low. My cost } for keeping a box on the net are 50 a month and hardware } replacement. $1,000 a year covers the cost including administration } after the first time set up. So very modest page charges would } be very attractive to and ISP. My interest is getting it started. } } My cost will go up with a lot of net acess. But not much. } } Gordon } Gordon Couger } 624 Cheyenne } Stillwater, OK 74075 } 405 624 2855 GMT - 6:00 } -----Original Message----- } } From: Sara Miller {saram-at-duke.edu} } To: Paul Webster {pwebster-at-mailhouse.hei.org} } Cc: MSA listserver submission {Microscopy-at-Sparc5.Microscopy.Com} } Date: Tuesday, September 14, 1999 4:22 PM } Subject: Re: Recommnedations } } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } Hey, a "Comsumer Reports" of microscopy equipment. Great idea. Are you } } volunteering to catalog them???? Maybe it's a project MSA would support } } with a small grant to keep the data base???? } } } } } } On 13 Sep 1999, Paul Webster wrote: } } } } } Date: 13 Sep 99 17:44:04 -0700 } } } From: Paul Webster {pwebster-at-mailhouse.hei.org} } } } To: MSA listserver submission {Microscopy-at-sparc5.microscopy.com} } } } Subject: Recommnedations } } } } } } ------------------------------------------------------------------------ } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } -----------------------------------------------------------------------. } } } } } } } } } Dear all, } } } } } } I notice lots of calls for recommendations of software, CCD cameras, } diamond knives and other microscopy-related equipment appearing on this } list. } } } } } } As I too am interested in comments about these products, and as I know } most replies occur off-line where I don't get to see them, wouldn't it be } great if there was a central site where we could submit our own reviews of } the equipment we use, and read other users comments? } } } } } } At one of the on-line book sellers, the site allows for submission of } book reviews. When added together, the sum of these reviews gives a new } reader a good idea of what to expect. } } } Couldn't we do this for our stuff too? In addition to giving the buyer } an informed view of expensive equipment, it might help suppliers identify } potential problems early enough to correct them. } } } } } } Any comments, volunteers etc.? } } } } } } Best regards, } } } } } } Paul Webster, Ph.D. } } } House Ear Institute } } } 2100 West Third Street } } } Los Angeles CA 90057 } } } 213 273 8026 } } } http://www.hei.org/htm/aemi.htm } } } } } } } } } } } } } } } } Sara E. Miller, Ph. D. } } P. O. Box 3020 } } Duke University Medical Center } } Durham, NC 27710 } } Ph: 919 684-3452 } } FAX: 919 684-8735 } } } } } }
There is an immediate opening for a Manager of a Materials Characterization Facility at the University of Pennsylvania, Philadelphia, PA. The facility is housed within the buildings of the Laboratory for Research on the Structure of Matter (LRSM) and is closely affiliated with the Department of Materials Science. The facility houses primary research equipment for electron microscopy and spectroscopy, ion scattering and scanned probe microscopy. Included in the equipment are a loaded FEG-TEM (JEOL 2010F), HREM (JEOL 4000), FEG-SEM (JEOL 6300F), thermionic SEM (JEOL 6400), STEM-TEM (Philips 400, likely upgraded soon), Scanning Auger (Phi) w/ SIMS, ion accelerator (NEC) w/ three beam lines and two AFMs (Digital).
The University of Pennsylvania is located in Philadelphia, one of the nation's most vibrant cities. The university, a member of the Ivy League, was founded by Ben Franklin and is the fourth oldest, and first secular, university in the US. The LRSM was constructed to house one of the original three Materials Research Laboratories (MRLs) in the US. The university has a continuing tradition of leading materials research and has housed the MRL (now MRSEC) continuously for 40 years.
The text of the official job listing from the University of Pennsylvania website follows. Please refer to the Penn Human Resources website for the official hiring policy of the university at www.hr.upenn.edu. For information about the specific position, please contact Professor David E. Luzzi at luzzi-at-lrsm.upenn.edu.
Text of website listing
Reference Number: 99083647DL Job Title: MANAGER D School/Center: ENGINEERING & APPLIED SCIENCE Department: MATERIALS CHARACTERIZATION Date Posted: 8/30/99 Salary Grade: 028 Minimum: $41,500.00 Top of First Third: $52,533.00 Top of Second Third: $63,566.00 Maximum: $74,600.00 Position Length: Ongoing
Duties: Manage Materials Characterization Facility/Service Center; develop short & long term use agreements with industrial & academic users; assist in experiments by users; compose & present reports bi-weekly to faculty oversight committee; coordinate work assignments of technical staff; assist or coordinate assistance of faculty in teaching & equipment acquisition; train or coordinate training of users; maintain or coordinate maintenance of equipment.
Qualifications: BA/BS in Physical Science or Engineering is required, advanced degree preferred; extensive experience in operation & use of transmission & scanning electron microscopes for imaging, diffraction & spectroscopy; knowledge of vacuum systems, ion scattering techniques, spectroscopic techniques & surface force techniques required.
My thanks to all who replied to my post. Thanks for all the good } suggestions, some of which were suggested by more than one person. My } question for Geoff Williams is this: how does one measure the osmolarity } of cytoplasm only or vacuolar contents only? I have just been grinding tissue, spinning it, and measuring osmolarity of the supernatent. Also, } these plants sequester NaCl in their vacuoles as part of their salt } tolerance mechanism. Don^Ot they require a balancing osmoticum in the } cytoplasm? I thought that was what prolines and betaines did. Do you have any references on this point? Thanks again. Mary
Thanks to all who offered suggestions. Chip Montrose came up with a number for a Fuji Tech line. We have called in the past and they have simply referred us to our worthless dealer. This time we explained our dealer wasn't helping so they connected us with a tech rep. The rep said we could open an account (free for telephone help) and be able to call directly in the future.
The rep then instantly diagnosed the problem as a sticky trip switch adjacent to the cutter for the donor paper. We confirmed this switch was sticking and have a new one on order. Thanks again (esp to Chip!). Tom
Thomas E. Phillips, Ph.D. Associate Professor of Biological Sciences Director, Molecular Cytology Core Facility
3 Tucker Hall Division of Biological Sciences University of Missouri Columbia, MO 65211-7400 (573)-882-4712 (voice) (573)-882-0123 (fax)
At 05:20 PM 9/14/99 , you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
[snip]
Another option is to host material and links to materials. Rather than taking on the work of absorbing and managing all material, one would be greatly relieved by only having to manage links. Most folks have web sites available to them. They can post their own materials at those sites and submit those specific URLs. Periodically, a snake can validate each URL for currency. For those folks who do not have web sites available, then of course they would submit the material.
I suspect that hosting only a mass of material could chew up a good sized chunk of disk space. Furthermore, unless the disk is regularly backed up, a crash means that all material will have to either be re-submitted or reconstructed from the last backup.
At 01:39 PM 9/12/99 , you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I asked this same question about a month ago. The resounding answer is yes, a SEM will image bacteria. But it is not easy. I'm working on some samples right now and can get good images at about 2,000X but above about 5,000X, the resolution falls off. I'm using a LaB6 instrument and it should have good resolution up to perhaps 70,000X. I think and hope that operator error is the main culprit right now. I'd like to fire the SEM operator but I'm the operator. So, the struggle goes on. But I think that I will succeed.
I would prefer links but I can store the data and the system is regulary backed up. It host some small international web pages and mailer. It is backed up weekly to a second on board hard drive downloaded to CDROM. The last disaster recovery took 45 minutes. A rebuild from CDROM would take a couple of hours right now. This will increase with more data.
With 10 gig drives at $300 and falling the cost of storage does not worry me much.
Gordon Gordon Couger 624 Cheyenne Stillwater, OK 74075 405 624 2855 GMT - 6:00 } } Another option is to host material and links to materials. Rather than taking } on the work of absorbing and managing all material, one would be greatly } relieved by only having to manage links. Most folks have web sites } available to them. They can post their own materials at those sites and } submit those specific URLs. Periodically, a snake can validate each } URL for currency. For those folks who do not have web sites available, } then of course they would submit the material. } } I suspect that hosting only a mass of material could chew up a good sized } chunk of disk space. Furthermore, unless the disk is regularly backed up, } a crash means that all material will have to either be re-submitted or } reconstructed from the last backup. } } gary g. }
I have got six or seven replies for my question on the sputter coater target. Most of them suggested to use a solvent or detergent type liquid like an alcohol, or an acetone. One of them suggested polishing, and another of them suggested to use an acid cleaning. I tried ultrasonic cleaning with acetone and got a reasonably good, even not the best, result. Still some red stuff was left, but the coater works OK.
To the people who responded to my question about using ESEM to look at slurry, thank you for your suggestions - I've taken note of your ideas and appreciate the help.
Lyn Waterhouse CEMMSA Centre for Electron Microscopy and Microstructure Analysis University of Adelaide Adelaide SA 5005 Ph: (08) 8303 4074 Fax: (08) 8303 4356
Chaps, Some time ago there was commercially produced a wall chart (Germany??) that displayed the different types of fracture surfaces (+ a few errors) as observed using an SEM and which was used as an aid / display by metallurgists. Does anyone out there have a similar wall chart in their SEM / Metallurgy lab or perhaps know any of the details of similar types of wall charts. Possibly, it is no longer available?? Thankyou for your help Barry M UNIT UNSW
Barry: I have the answer to your query. One of these charts is hanging in my lab. Here are the detail: Title: FRACTOGRAPHY IN MATERIALS SCIENCE AND ENGINEERING published by ASM International, The Materials Information Society, Materials Park, OH 44073-0002 USA Tel: 216-338-5151 Fax: 216-338-4634
The chart has been photographed and designed by Mohan Chaudhari, Ph.D.,P.E Columbus Metallurgical Services,Inc. 4348 Reynolds Drive Hillard, OH 43026-1260 USA Tel: 614-529-1311 Fax: 614-529-1818 If you have any difficulty please let me know off line. I know Mohan well.
Vin Berry Analytical Technology, GE Plastics, Washington, WV 26181 Tel: 304-863-7528, fax -7108 GE Dial: 8*572-7528 e.mail: vinod.berry-at-gep.ge.com
-----Original Message----- } From: Barry Searle [mailto:B.Searle-at-unsw.edu.au] Sent: Thursday, September 16, 1999 4:17 AM To: microscopy-at-sparc5.microscopy.com
Chaps, Some time ago there was commercially produced a wall chart (Germany??) that displayed the different types of fracture surfaces (+ a few errors) as observed using an SEM and which was used as an aid / display by metallurgists. Does anyone out there have a similar wall chart in their SEM / Metallurgy lab or perhaps know any of the details of similar types of wall charts. Possibly, it is no longer available?? Thankyou for your help Barry M UNIT UNSW
I am in need of the specimen cassette holder (part no 0303) for an A/S 325 microtome. I have tried contacting the company direct but get no response, so i am asking you all whether any of you have one of these microtomes sitting in a corner somewhere gathering dust and would be prepared to part with the holder. If not does any one know where i may be able to obtain one?
Cheers Phil Mutch
Mr Philip Mutch, School of Biomedical Science, E Floor Medical School, University of Nottingham, Nottingham, NG7 2UH. UK. E-mail Philip.Mutch-at-nottingham.ac.uk
While I agree that traditional publication procedures are slow and cumbersome, they do provide an important service: reviewing. Without review, the quality of the publications will quickly degrade to a point that makes publishing useless. Just check out the physics newsgroups on the net. When I last looked there, there were a number of whackos who were arguing for the weirdest ideas (Earth core is made of strawberry jelly kind of thing). I am afraid, that these people would quickly take over any unreviewed publishing site, and make it completely useless for scientific publications.
Also look at the scientific journals: the ones with the strictest screening are in general respected the most.
I would think, that in order to provide a value to the science community, it is a good idea to go digital on the publications WITHOUT sacrificing the quality of the publications, i.e., without giving up reviewing.
I remember a few years back there was a big uproar in the physics community about some research that was published in the New York Times before it made it into a scientific journal (I forgot, what exactly it was. Cold Fusion??).
Just my thoughts...
Michael
Michael Bode, Ph.D. Soft Imaging System Corp. 1675 Carr St., #105N Lakewood, CO 80215 =================================== phone: (888) FIND SIS (303) 234-9270 fax: (303) 234-9271 email: mailto:info-at-soft-imaging.com web: http://www.soft-imaging.com ===================================
} ---------- } From: Gordon Vrololjak[SMTP:GVRDOLJA-at-NATURE.BERKELEY.EDU] } Sent: Wednesday, September 15, 1999 11:50:57 AM } To: Gordon Couger } Cc: Sara Miller; Paul Webster; MSA listserver submission } Subject: Re: Recommnedations } Auto forwarded by a Rule } ------------------------------------------------------------------------ The Microscopy ListServer -- Sponsor: The Microscopy Society of America
I totally agree with this, Journal review is taking far too long now. Scientists should really put more effort in reviewing their co-workers paper submissions. Papers sitting on someone's desk for six months without review is unacceptable. I personally try to finish a review once I receive it within two weeks. Many people asked to review papers often never reply to the publishers, which I think is a serious offence in the scientific field.
I know the NIH is considering doing rapid article publication on their website. After screening of the articles, they are placed onto the web for anyone to download or read. I think there are other journals going this route as well. It is the communication method of the future. I think that publications will go this way. More respected online journals will have extensive peer review and screening. Other rapid publication journals will have less, and still be immensely useful in being able to do online searching for specific information.
I have a lot of experience in programming, web design, and system administration. I am interested in forming a rapid publication web site for the scientific community and will readily offer my skills and time. Gordon Vrdoljak.
\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/ \/\/\ Gordon Ante Vrdoljak 1 Cyclotron Road ICQ 23243541 http://nature.berkeley.edu/~gvrdolja MS90-1116 GAVrdoljak-at-lbl.gov Ernest Orlando phone (510) 495-2829 Lawrence Berkeley fax (510) 486-7797 National Laboratory cell (510) 290-6793 Berkeley CA 94720
On Tue, 14 Sep 1999, Gordon Couger wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I have the space to keep the data base. I don't have the expertise } to edit it. It is a pretty good link. It is not as fast as the best but } the congestion is low enough it makes up for it most of the time. } } I will consider any form of internet publication. I am looking for } a small circulation juried journal. I am primarily interested in } increasing the speed of information distribution and reducing } the cost. } } My experience with some of my own publications where it took } a year to get it published after acceptance and $700 bucks for } reprints and they kept the copyright left a bad taste in my mouth. } } If we are going to increase the rate of progress in technology we } need to get the time to publication down as low as possible. I think } it should be possible to get a non controversial jurried article out } in two months and letters out in hours. } } I am interested in publishing only. I am no editor. All copyrights } stay with the author and no page cost at this point. The cost of } publication on the internet is very low. My cost } for keeping a box on the net are 50 a month and hardware } replacement. $1,000 a year covers the cost including administration } after the first time set up. So very modest page charges would } be very attractive to and ISP. My interest is getting it started. } } My cost will go up with a lot of net acess. But not much. } } Gordon } Gordon Couger } 624 Cheyenne } Stillwater, OK 74075 } 405 624 2855 GMT - 6:00 } -----Original Message----- } } From: Sara Miller {saram-at-duke.edu} } To: Paul Webster {pwebster-at-mailhouse.hei.org} } Cc: MSA listserver submission {Microscopy-at-Sparc5.Microscopy.Com} } Date: Tuesday, September 14, 1999 4:22 PM } Subject: Re: Recommnedations } } } } ----------------------------------------------------------------------- - } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------
Can anyone help us with fixing our LKB Utrotome IIIs by faxing circuit diagram? Much appreciated.
Marek Malecki, M.D., Ph.D. Director and Principal Investigator
Molecular Imaging Laboratories (MIL) and Department of Biology University of California at San Diego
address: 1500 Bonner Hall, University of California at San Diego 9500 Gilman Drive, La Jolla, CA 92093-0368 telephone - lab: 8585342484 telephone - office: 8588223373 pager: 8586161420 fax: 8588223715 email: mmm-at-biomail.ucsd.edu www site: http://mil.ucsd.edu
I am seeking your help in preparing a multimeric protein (800kDa) -oligomeric form. I used a single C layer film + negative stain (UA) and would like to know if any one has used a double C layer technique. Rosemary
We will replace our primary SEM with a new ESEM in a few months. Consequently, some of our older instrumentation will become available for excess, surplus, or some other sort of disposition (spare parts?). Please e-mail if there is there any interest for the following items? Note: the items will be disposed by the usual official government protocols.
1. Cambridge S-250 SEM, was working well until CRT problem this past May (horizontal line showing on screen). May be corrected for $5000-$10000 by Leo, Inc. although some parts are not available any longer.
2. Tracor-Northern 2010 EDS, still functional as of May, 1999. Replaced CPU board this past January.
3. Microspec WDX-2A with detector, probably functional. Since Oxford can upgrade these WDS units, we may keep it. Not my decision.
Bruce F. Ingber Biologist- Electron Microscopy USDA-ARS, SRRC 1100 Robert E. Lee Blvd. New Orleans, LA 70124-4305
Dear Listers, I'm lookling for a commercial source in the US for a low viscosity vinyl silicone (dental impression material). I found a commercial product in a paper -GC EXAFLEX. It was obtained from a GC Dental Industries Corp in Japan. Rosemary
Our Ohio company is seeking engineering / scientist support for analysis of chemistry and microstructure of solid lubricant and hard coatings used in lubrication of aerospace systems. Work will involve preparing SEM & TEM specimens of thin films and wear scars on steel and ceramic substrates; correlating thin film properties with deposition plasma characteristics; and making recommendations for improving lifetime and performance of such materials in different environments: e.g., vacuum, moist air, high temperature, etc.
It is important that candidates have capabilities in cross-section TEM, analytical TEM, analysis of unique microstructures; and understand TEM of thin films on a fundamental level. It is desirable that the candidate have knowledge of tribological materials and experience with TEM/XTEM of wear tracks.
Contact Ronald Decker - mailto:decker-at-utcdayton.com
{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} Ronald C. Decker Program Manager Universal Technology Corporation 1270 N FAIRFIELD RD DAYTON OH 45432-2600
Voice (937) 426-8530, Fax (937) 426-7753 (Voice mail is available at my extension, 270)
SEM Fractographs by Svend Engell-Nielson The Technological Institute DK - 2630 Tastrup, Denmark =AE1975
J. Roy Nelson Material Testing Laboratory Pennington, NJ 08534
"Barry Searle (by way of Nestor J. Zaluzec)" wrote:
} -----------------------------------------------------------------------= - } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Co= m } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.htm= l } -----------------------------------------------------------------------. } } Chaps, Some time ago there was commercially produced a wall cha= rt } (Germany??) that displayed the different types of fracture surfaces (+= a } few errors) as observed using an SEM and which was used as an aid / dis= play } by metallurgists. Does anyone out there have a similar wall chart in } their SEM / Metallurgy lab or perhaps know any of the details of simila= r } types of wall charts. Possibly, it is no longer available?? Thanky= ou } for your help Barry M UNIT UNSW
These materials should be available from any good dental supply agent we have used various polyvinylsiloxane materials: Extrude (polyvinylsiloxane) (Kerr Manufacturing Co, Romulusm MI 48174) and Express (3M Dental Products Division, St Paul, MN 55144- 1000) this latter had a note on the box - U.S. Federal Law restricts this device to sale by or on the order of a dental professional.
Hope this helps
Ian
} Dear Listers, } I'm lookling for a commercial source in the US } for a low viscosity vinyl silicone (dental impression material). } I found a commercial product in a paper -GC EXAFLEX. It } was obtained from a GC Dental Industries Corp in Japan. } Rosemary } } }
Ian Hallett HortResearch Mt Albert Research Centre Private Bag 92 169 Auckland, New Zealand Fax 64-9-815 4201 Telephone 64-9-815 4200 EMail ihallett-at-hort.cri.nz
Hi all, We have just a biopsy arrive in our lab, which is a platelet sample in paraffin wax. Now we were wondering, do we just de-paraffinize as usual in xylene, but spinning it down between each change? Then spinning it down at each re-hydration step til into buffer where when we can embed it into agarose or something like that?
We havent had one of these before, and as usual, would hate to loose what precious little we have been given!
Would appreciate hearing from people who have done this before,
Ta!!
Rich.
----------------------------------------------------------------------- Richard Lander Electron Microscopist South Campus Electron Microscope Unit Otago School of Medical Sciences P.O. Box 913 Dunedin New Zealand. Tel. National 03 479 7301 Fax. National 03 479 7254 mailto:richard.lander-at-stonebow.otago.ac.nz http://www.otago.ac.nz/anatomy/emunit/ ------------------------------------------------------------------------
I agree with this and would like to add another concern. In library school, my rare books professor would always say, "Bibliographic description forms the basis of textual criticism", meaning that you have to have a way of identifying the item that you are commenting on so that all scholarly discussion of the publication is 'on the same wavelength'. So if you have links to publications on people's own servers and they decide to make a few updates here and there, you have to have a way of identifying the different versions of the documents and this method should be standardized. Having the publications go through a centralized review process and having static versions available on a server or group of mirror servers is preferable, I think.
I don't know what the NIH project is going to be like, but I'm looking forward to it, considering what a success Medline is. Another project to watch for is SPARC http://www.arl.org/sparc
Electronic publishing might provide the opportunity to come up with solutions to the problems of lag time and undue cost. However, electronic publishing in and of itself is not the answer (some ejournals are very expensive and e-publications can suffer massive delays just like print).
Back to the original subject of the post: an online site for product reviews and information sounds like a very good idea.
PS - An entertaining account of the "cold fusion" debacle (as well as "Biosphere II" and others) can be found in the book _Yes, We Have No Neutrons..._
On Thu, 16 Sep 1999, Michael Bode wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Let me throw in a few thoughts... } } While I agree that traditional publication procedures are slow and } cumbersome, they do provide an important service: reviewing. Without } review, the quality of the publications will quickly degrade to a point } that makes publishing useless. Just check out the physics newsgroups on } the net. When I last looked there, there were a number of whackos who } were arguing for the weirdest ideas (Earth core is made of strawberry } jelly kind of thing). I am afraid, that these people would quickly take } over any unreviewed publishing site, and make it completely useless for } scientific publications. } } Also look at the scientific journals: the ones with the strictest } screening are in general respected the most. } } I would think, that in order to provide a value to the science } community, it is a good idea to go digital on the publications WITHOUT } sacrificing the quality of the publications, i.e., without giving up } reviewing. } } I remember a few years back there was a big uproar in the physics } community about some research that was published in the New York Times } before it made it into a scientific journal (I forgot, what exactly it } was. Cold Fusion??). } } Just my thoughts... } } Michael } } Michael Bode, Ph.D. } Soft Imaging System Corp. } 1675 Carr St., #105N } Lakewood, CO 80215 } =================================== } phone: (888) FIND SIS } (303) 234-9270 } fax: (303) 234-9271 } email: mailto:info-at-soft-imaging.com } web: http://www.soft-imaging.com } =================================== } } } } } ---------- } } From: Gordon Vrololjak[SMTP:GVRDOLJA-at-NATURE.BERKELEY.EDU] } } Sent: Wednesday, September 15, 1999 11:50:57 AM } } To: Gordon Couger } } Cc: Sara Miller; Paul Webster; MSA listserver submission } } Subject: Re: Recommnedations } } Auto forwarded by a Rule } } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } I totally agree with this, } Journal review is taking far too long now. Scientists should really put } more effort in reviewing their co-workers paper submissions. Papers } sitting on someone's desk for six months without review is unacceptable. } I personally try to finish a review once I receive it within two weeks. } Many people asked to review papers often never reply to the publishers, } which I think is a serious offence in the scientific field. } } I know the NIH is considering doing rapid article publication on their } website. After screening of the articles, they are placed onto the web } for anyone to download or read. I think there are other journals going } this route as well. It is the communication method of the future. I } think that publications will go this way. More respected online } journals } will have extensive peer review and screening. Other rapid publication } journals will have less, and still be immensely useful in being able to } do } online searching for specific information. } } I have a lot of experience in programming, web design, and system } administration. I am interested in forming a rapid publication web site } for the scientific community and will readily offer my skills and time. } Gordon Vrdoljak. } } \/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/ } \/\/\ } Gordon Ante Vrdoljak 1 Cyclotron Road } ICQ 23243541 http://nature.berkeley.edu/~gvrdolja MS90-1116 } GAVrdoljak-at-lbl.gov Ernest Orlando } phone (510) 495-2829 Lawrence } Berkeley } fax (510) 486-7797 National } Laboratory } cell (510) 290-6793 Berkeley CA } 94720 } } On Tue, 14 Sep 1999, Gordon Couger wrote: } } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of } America } } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } -----------------------------------------------------------------------. } } } } } } I have the space to keep the data base. I don't have the expertise } } to edit it. It is a pretty good link. It is not as fast as the best } but } } the congestion is low enough it makes up for it most of the time. } } } } I will consider any form of internet publication. I am looking for } } a small circulation juried journal. I am primarily interested in } } increasing the speed of information distribution and reducing } } the cost. } } } } My experience with some of my own publications where it took } } a year to get it published after acceptance and $700 bucks for } } reprints and they kept the copyright left a bad taste in my mouth. } } } } If we are going to increase the rate of progress in technology we } } need to get the time to publication down as low as possible. I think } } it should be possible to get a non controversial jurried article out } } in two months and letters out in hours. } } } } I am interested in publishing only. I am no editor. All copyrights } } stay with the author and no page cost at this point. The cost of } } publication on the internet is very low. My cost } } for keeping a box on the net are 50 a month and hardware } } replacement. $1,000 a year covers the cost including administration } } after the first time set up. So very modest page charges would } } be very attractive to and ISP. My interest is getting it started. } } } } My cost will go up with a lot of net acess. But not much. } } } } Gordon } } Gordon Couger } } 624 Cheyenne } } Stillwater, OK 74075 } } 405 624 2855 GMT - 6:00 } } -----Original Message----- } } } From: Sara Miller {saram-at-duke.edu} } } To: Paul Webster {pwebster-at-mailhouse.hei.org} } } Cc: MSA listserver submission {Microscopy-at-Sparc5.Microscopy.Com} } } Date: Tuesday, September 14, 1999 4:22 PM } } Subject: Re: Recommnedations } } } } } } } } ----------------------------------------------------------------------- } - } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of } America } } } To Subscribe/Unsubscribe -- Send Email to } ListServer-at-MSA.Microscopy.Com } } } On-Line Help } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } } ----------------------------------------------------------------------- }
Hi All I have recently had my EDS detector repaired and upgraded from a Be window to an ATW window. I am in the process of recalibrating standards for use in ZAF/PB on an AN 10000 system. I have successfully calibrated elements from Na upwards. I have succesfully created profiles for C , N and O but when trying to calibrate the standard to derive FST and RST the system crashes with errors. Is anyone sucessfully using ZAF/PB with a light element detector? Or has anyone any idea why the system might object to light element quant? Many thanks
Chris
Chris Gilpin Experimental Officer Biological Sciences EM Unit G452 Stopford Building Oxford Road Manchester M13 9PT phone +44 0161 275 5170 Fax +44 0161 275 5171 http://www.empgu.man.ac.uk
Dear All, I enclose details of a post-doc vacancy at the University of Barcelona, Spain. The starting date would be about April-May 2000, so we have extended the deadline for applications. Any one interested please reply directly to paqui-at-el.ub.es and/or send applications and a CV by mail before 30 November 1999.
Kind regards
F. Peir=F3
**************************************************************************= ** Laboratory: Electronic Materials and Engineering, Department of Electronics, University of Barcelona.
Duration: 12-18 months, starting April-May 1999.
Raising the subject yet again:
We are seriously considering purchasing a scanner for negatives ranging from 35mm (from optical mic., SEM, Philips 310) and plates about 3x4" (from Philips CM20).
I've looked through the archive, and come up with recommendations for:
Agfa Duoscan (web page kindly provided) Polaroid Sprintscan 45 Nikon LS-4500 for $6500 ** Minolta Dimage Scan Multi for $2500 ** UMAX (generally)
** (from Publishing Perfection)
People over this side of the pond have generally recommended Heidelberg "Saphir" or similar.
Could anybody give me up-to-date recommendations, in particluar addressing the question as to whether it makes sense to have (1) a dedicated scanner for negatives and one for A4 (2) one to do everything.
Solution 1 recommends itself, as the prints, etc, to be scanned might be sometimes be a little grubby from storage.
Pointers to WEB SITES would be much appreciated.
Thanks in advance,
+------------------------------------------------------------------------+ | Robert H.Olley Phone: | | J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 | | University of Reading {University internal extension 7867 | | Whiteknights Fax +44 (0) 118 9750203 | | Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk | | England URL: http://www.reading.ac.uk/~spsolley | +------------------------------------------------------------------------+
Robin Cross emailed the diagram. We have the unit fixed. Cordial thanks to all who replied.
Amazing place. Amazing people.
Marek Malecki, M.D., Ph.D. Director and Principal Investigator
Molecular Imaging Laboratories (MIL) and Department of Biology University of California at San Diego
address: 1500 Bonner Hall, University of California at San Diego 9500 Gilman Drive, La Jolla, CA 92093-0368 telephone - lab: 8585342484 telephone - office: 8588223373 pager: 8586161420 fax: 8588223715 email: mmm-at-biomail.ucsd.edu www site: http://mil.ucsd.edu
Your AS 325 Microtome was produced by Anglia Scientific who were taken over by Shandon who in turn became part of Life Sciences International. The last address I have for them is as follows:
Life Sciences International (Europe) Ltd 93-96 Chadwick Road Astmoor Runcorn Cheshire WA7 1PR Tel 01928 566611 Fax 01928 565845
Many microtomes have drifted under the bridge since these changes but if you have any problems please contact me and I may be able to trace something,
Regards
Terry Cooper TAAB Laboratories Equipment Ltd 3 Minerva House, Calleva Park Aldermaston, Berks, RG7 8NA, UK
Postdoctoral Position to Develop Advanced Microscopy Capabilities in a Microscopy User Center
The Materials Analysis User Center (MAUC) at the Oak Ridge National Laboratory in Oak Ridge, Tennessee, is seeking a postdoctral candidate to perform research to facilitate the use of electron microscopes remotely, and to improve TEM resolution using electron beam monochromators and hardware aberration correctors.
There are two primary areas that the person selected for this position will be expected to contribute. First, this center and the Oak Ridge National Laboratory have strongly supported the concept of a virtual electron microscopy laboratory over the last years and will continue to move in that direction. Second, in support Department of Energy research programs aimed at reducing both gaseous and particulate emissions from gasoline and diesel engines, the ultimate in TEM atomic resolution (at intermediate voltages) for nano-particles and other materials is a critical need for this center.
The successful candidate should have a PhD in physics (although other disciplines will be considered, depending upon overall qualifications). The candidate must have a strong background in all of the following: (1) electron optics (preferably in the design and use of electron monochromators and aberration correctors), (2) programming skills including C and C++ (Java experience is desired), and (3) hands-on experience with TEM and STEM instrumentation. The researcher will work with many DOE contractors, industrial and university researchers, including some as a part of DOE-ORNL user programs; thus he or she should enjoy collaborating with others. This position is for one year, extendable to two.
ORNL, a multipurpose research laboratory managed by Lockheed Martin Energy Research Corporation for the U.S. Department of Energy, is an equal opportunity employer committed to building and maintaining a diverse work force.
Please send curriculum vitae and bibliography to:
Ted Nolan Manager, Materials Analysis User Center High Temperature Materials Laboratory Oak Ridge National Laboratory 1 Bethel Valley Road Bldg. 4515, MS 6064 Oak Ridge, TN 37831-6064
Also very narrow groups could get wider representation. Cross feild porject have been the best project I have worked on.
Gordon
} } I agree with this and would like to add another concern. } In library school, my rare books professor would always say, } "Bibliographic description forms the basis of textual criticism", } meaning that you have to have a way of identifying the item that you are } commenting on so that all scholarly discussion of the publication is } 'on the same wavelength'. So if you have links to publications on people's } own } servers and they decide to make a few updates here and there, you have to } have a way of identifying the different versions of the documents and this } method should be standardized. Having the publications go through a } centralized review process and having static versions available on a } server or group of mirror servers is preferable, I think. } } I don't know what the NIH project is going to be like, but I'm looking } forward to it, considering what a success Medline is. Another project to } watch for is SPARC } http://www.arl.org/sparc } } Electronic publishing might provide the opportunity to come up } with solutions to the problems of lag time and undue cost. However, } electronic publishing in and of itself is not the answer (some ejournals } are very expensive and e-publications can suffer massive delays just like } print). } } Back to the original subject of the post: } an online site for product reviews and information sounds like a very good } idea. } } PS - An entertaining account of the "cold fusion" debacle } (as well as "Biosphere II" and others) can be found in the book } _Yes, We Have No Neutrons..._ } } On Thu, 16 Sep 1999, Michael Bode wrote: } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } Let me throw in a few thoughts... } } } } While I agree that traditional publication procedures are slow and } } cumbersome, they do provide an important service: reviewing. Without } } review, the quality of the publications will quickly degrade to a point } } that makes publishing useless. Just check out the physics newsgroups on } } the net. When I last looked there, there were a number of whackos who } } were arguing for the weirdest ideas (Earth core is made of strawberry } } jelly kind of thing). I am afraid, that these people would quickly take } } over any unreviewed publishing site, and make it completely useless for } } scientific publications. } } } } Also look at the scientific journals: the ones with the strictest } } screening are in general respected the most. } } } } I would think, that in order to provide a value to the science } } community, it is a good idea to go digital on the publications WITHOUT } } sacrificing the quality of the publications, i.e., without giving up } } reviewing. } } } } I remember a few years back there was a big uproar in the physics } } community about some research that was published in the New York Times } } before it made it into a scientific journal (I forgot, what exactly it } } was. Cold Fusion??). } } } } Just my thoughts... } } } } Michael } } } } Michael Bode, Ph.D. } } Soft Imaging System Corp. } } 1675 Carr St., #105N } } Lakewood, CO 80215 } } =================================== } } phone: (888) FIND SIS } } (303) 234-9270 } } fax: (303) 234-9271 } } email: mailto:info-at-soft-imaging.com } } web: http://www.soft-imaging.com } } =================================== } } } } } } } } } ---------- } } } From: Gordon Vrololjak[SMTP:GVRDOLJA-at-NATURE.BERKELEY.EDU] } } } Sent: Wednesday, September 15, 1999 11:50:57 AM } } } To: Gordon Couger } } } Cc: Sara Miller; Paul Webster; MSA listserver submission } } } Subject: Re: Recommnedations } } } Auto forwarded by a Rule } } } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } I totally agree with this, } } Journal review is taking far too long now. Scientists should really put } } more effort in reviewing their co-workers paper submissions. Papers } } sitting on someone's desk for six months without review is unacceptable. } } I personally try to finish a review once I receive it within two weeks. } } Many people asked to review papers often never reply to the publishers, } } which I think is a serious offence in the scientific field. } } } } I know the NIH is considering doing rapid article publication on their } } website. After screening of the articles, they are placed onto the web } } for anyone to download or read. I think there are other journals going } } this route as well. It is the communication method of the future. I } } think that publications will go this way. More respected online } } journals } } will have extensive peer review and screening. Other rapid publication } } journals will have less, and still be immensely useful in being able to } } do } } online searching for specific information. } } } } I have a lot of experience in programming, web design, and system } } administration. I am interested in forming a rapid publication web site } } for the scientific community and will readily offer my skills and time. } } Gordon Vrdoljak. } } } } \/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/ } } \/\/\ } } Gordon Ante Vrdoljak 1 Cyclotron Road } } ICQ 23243541 http://nature.berkeley.edu/~gvrdolja MS90-1116 } } GAVrdoljak-at-lbl.gov Ernest Orlando } } phone (510) 495-2829 Lawrence } } Berkeley } } fax (510) 486-7797 National } } Laboratory } } cell (510) 290-6793 Berkeley CA } } 94720 } } } } On Tue, 14 Sep 1999, Gordon Couger wrote: } } } } } } } ------------------------------------------------------------------------ } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of } } America } } } To Subscribe/Unsubscribe -- Send Email to } } ListServer-at-MSA.Microscopy.Com } } } On-Line Help } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } } } -----------------------------------------------------------------------. } } } } } } } } } I have the space to keep the data base. I don't have the expertise } } } to edit it. It is a pretty good link. It is not as fast as the best } } but } } } the congestion is low enough it makes up for it most of the time. } } } } } } I will consider any form of internet publication. I am looking for } } } a small circulation juried journal. I am primarily interested in } } } increasing the speed of information distribution and reducing } } } the cost. } } } } } } My experience with some of my own publications where it took } } } a year to get it published after acceptance and $700 bucks for } } } reprints and they kept the copyright left a bad taste in my mouth. } } } } } } If we are going to increase the rate of progress in technology we } } } need to get the time to publication down as low as possible. I think } } } it should be possible to get a non controversial jurried article out } } } in two months and letters out in hours. } } } } } } I am interested in publishing only. I am no editor. All copyrights } } } stay with the author and no page cost at this point. The cost of } } } publication on the internet is very low. My cost } } } for keeping a box on the net are 50 a month and hardware } } } replacement. $1,000 a year covers the cost including administration } } } after the first time set up. So very modest page charges would } } } be very attractive to and ISP. My interest is getting it started. } } } } } } My cost will go up with a lot of net acess. But not much. } } } } } } Gordon } } } Gordon Couger } } } 624 Cheyenne } } } Stillwater, OK 74075 } } } 405 624 2855 GMT - 6:00 } } } -----Original Message----- } } } } From: Sara Miller {saram-at-duke.edu} } } } To: Paul Webster {pwebster-at-mailhouse.hei.org} } } } Cc: MSA listserver submission {Microscopy-at-Sparc5.Microscopy.Com} } } } Date: Tuesday, September 14, 1999 4:22 PM } } } Subject: Re: Recommnedations } } } } } } } } } } } } ----------------------------------------------------------------------- } } - } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of } } America } } } } To Subscribe/Unsubscribe -- Send Email to } } ListServer-at-MSA.Microscopy.Com } } } } On-Line Help } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } } } } } ----------------------------------------------------------------------- } } } } } }
Robert, I use a Linotype-Hell Saphir Ultra (purchased before Linotype-Hell was bout out by Heidelberg Press). Very nice machine, but: 1) There is no provision (at least in the version of software that I have) for setting the darkest and brightest parts of the image in preview. You just have to go with whatever automatic correction the software uses. 2) I have had some reliability problems, the power supply died about three months after I bought it and it still has an intermittent fault in transparency mode where the light source above and CCD array below do not align correctly - giving poor quality images.
I used an Agfa some years ago which had (1) and it was much easier to use for scanning TEM negatives. I don't know whether my problems (2) are just for my machine or a reflection on the machines generally. I believe that the CCD and mechanical parts are from UMAX (also the case for Agfa)? I also believe that the maximum OD and optical (not interpolated) resolution has increased somewhat since I got my scanner about 18 months ago.
Despite the niggles, it is a very good and generally reliable machine which must have scanned about 2000 pictures in the last 18 months. It is used for everything from TEM negatives and X-ray radiographs to taking pictures from old reports (and of course holiday/wedding/old family photos out of hours!)
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } } Raising the subject yet again: } } We are seriously considering purchasing a scanner for negatives ranging } from 35 mm (from optical mic., SEM, Philips 310) and plates about 3x4" } (from Philips CM20). } } I've looked through the archive, and come up with recommendations for: } } Agfa Duoscan (web page kindly provided) } Polaroid Sprintscan 45 } Nikon LS-4500 for $6500 ** } Minolta Dimage Scan Multi for $2500 ** } UMAX (generally) } } ** (from Publishing Perfection) } } People over this side of the pond have generally recommended Heidelberg } "Saphir" or similar. } } Could anybody give me up-to-date recommendations, in particular addressing } the question as to whether it makes sense to have } (1) a dedicated scanner for negatives and one for A4 } (2) one to do everything. } } Solution 1 recommends itself, as the prints, etc., to be scanned might be } sometimes be a little grubby from storage. } } Pointers to WEB SITES would be much appreciated. } } Thanks in advance, } } +------------------------------------------------------------------------+ } | Robert H.Olley Phone: | } | J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 | } | University of Reading {University internal extension 7867 | } | Whiteknights Fax +44 (0) 118 9750203 | } | Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk | } | England URL: http://www.reading.ac.uk/~spsolley | } +------------------------------------------------------------------------+ } }
I have an EDS system with a 30 mm premium detector which had original spec's of 136 eV. The crystal pack needed repair and was replaced. The resolution now is 142 eV. I need to decide a course of action. Here are my options.
1. Keep the repaired 30 mm detector at 142 eV and be back up and running. 2. Here the vendor replace the crystal pack until it meets the 136 eV spec, which will take time and I might be down for a while. 3. Go to a 10 mm detector that I am told they can insure a resolution of 136 or better.
Question for the group, if I can live with the down time should I hold out for the 30 mm detector at 136eV?
Michael Ingram Rodel, Inc. 451 Bellevue RD Newark, DE 19713 Phone: 302.366.0500, ext. 2545 Fax: 302.455.1124
Does anyone in the Rochester, NY area have a JEOL 5800 SEM (or similar conventional SEM)? I will be in that region and I must test some equipment, so I need to gain access to a SEM similar to mine for a short period.
Please contact me off-line if this is possible, and for further info. Marisa Ahmad
I am rather surprised this a.m. to arrive at the lab to find that the = blocks I've embedded in LR White are not polymerized. This is not a = technique we routinely use. I followed the schedule for EM ICC, tissues = are .25-.5mm, no OSO4, ETOH dehydration to 95%, several infiltration = changes over hours and overnight, oven calibrated to 50 degrees, gelatin = capsules totally filled and capped, in the oven since Friday. Help -- What's going wrong?? What to do now ?? Linda Fox lfox1-at-wpo.it.luc.edu
I have a Gatan 600 Duo Mill Ion Miller of the 1985 vintage.
The Thermocouple gauge controller is malfunctioning and will not allow the difusion pump to turn on. It is the gauge on the right side of the miller. The gauge model number is CVH-3.
If anyone has the circiuit diagram of the interior of the controller I would appreciate a copy.
It can be faxed to (905) 521-2773. When faxing please use fine resolution since detail on the diagram is rather small.
Thanks in advance
Fred
******************************************************** Fred Pearson Brockhouse Institute for Materials Research McMaster University 1280 Main St. West Hamilton, Ontario Canada L8S 4M1
Wow, 136 eV with a 30 mm detector is exceptional, and I can see why you are a little bummed out that the repair came back 6 eV worse. But is that 6 eV important to you? I would guess that in practice you won't see much difference. Personally I would rather have the big detector.
best regards, mark
Mark W. Lund, PhD VP Engineering } } Soft X-ray Web page http://www.moxtek.com { { MOXTEK, Inc. 452 West 1260 North Orem UT 84057 801-225-0930 FAX 801-221-1121 lundm-at-xray.byu.edu
"This is a YOUNG business...How can I tell you what YOUR job is when I don't know what MINE is?" --Pogo
Mike Ingram wrote:
:I need opinions. : :I have an EDS system with a 30 mm premium detector which had original spec's :of 136 eV. The crystal pack needed repair and was replaced. The resolution :now is 142 eV. I need to decide a course of action. Here are my options. : :1. Keep the repaired 30 mm detector at 142 eV and be back up and :running. :2. Here the vendor replace the crystal pack until it meets the 136 eV :spec, which will take time and I might be down for a while. :3. Go to a 10 mm detector that I am told they can insure a resolution :of 136 or better. : :Question for the group, if I can live with the down time should I hold out :for the 30 mm detector at 136eV? : :Michael Ingram :Rodel, Inc. :451 Bellevue RD :Newark, DE 19713 :Phone: 302.366.0500, ext. 2545 :Fax: 302.455.1124
Hello every one Here is a quandary for you If you HAD to keep tissue (in this case 50 micron vibratome sections) at either the primary fixation step (2% PF and 2% Glut), or in a buffer at 4 degrees C after the primary fix. Which would be the most stable over several weeks time? Lets assume they will be looking for general ultrastructure. I know there are lots of variables but I'm looking for an "in general" answer.
I get request from people to do EM but I can not get their sample processed into resin at the time, so here I have a sample to store or in other cases they want to store it until they know if they will need the EM.
In the above experiment, another "EM" investigator told them to store the sample in a phosphate buffer at 4 degree. Due to the number of samples I won't be able to process them all at once so I'm not sure whether to leave them the way they are or transfer them back into fix. I personally think it's best to leave the sample in the primary fix at 4 degree. In the Path EM lab we used to keep samples this way, at room temp, and the tissue showed no discernible loss of ultrastructure even when stored a month or two. Thanks for your advice.
At 09:51 AM 9/20/99 -0500, you wrote: } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
****************************** If you post fix in osmium, you might carry it to the buffer rinse after the osmium step and store it in buffer at 4C. The tissue isn't fixed completely until it has been osmicated. This way both proteins and lipids will be well preserved.
Joiner Cartwright, Jr., Ph.D. Assistant Professor of Pathology Baylor College of Medicine Houston, Texas U.S.A.
I had the similar problem once before. I checked the LR white resin (which is newly purchased) withOUT sample and polymerized overnight at 50 or 70 C. It did NOT polymerize either. I called the supplier (EMS) and they sent me a new one. It worked fine?!
Linda Fox wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } I am rather surprised this a.m. to arrive at the lab to find that the blocks I've embedded in LR White are not polymerized. This is not a technique we routinely use. I followed the schedule for EM ICC, tissues are .25-.5mm, no OSO4, ETOH dehydration to 95%, several infiltration changes over hours and overnight, oven calibrated to 50 degrees, gelatin capsules totally filled and capped, in the oven since Friday. } Help -- What's going wrong?? What to do now ?? } Linda Fox } lfox1-at-wpo.it.luc.edu
Zhaojie Zhang Program in Molecular and Cell Biology Oklahoma Medical Research Foundation 825 NE 13th Street Oklahoma City, OK 73104
Dear Michael and Mark, 136 eV is not so exceptional if you are willing to put up with the long process times and high dead time %. If you are getting 142 eV with the longest process time, it might be worth it to wait for a better detector. I don't think the resolution is as important as the loss in counts and time you will incurr. We don't set our detector at the highest resolution so that we can get more counts per real time. It makes a difference for X-ray mapping where every nanosecond counts. You may also twist their arm into giving you a loaner. Ciao for now, Ken
} Dear Michael, } } Wow, 136 eV with a 30 mm detector is exceptional, and I can see } why you are a little bummed out that the repair came back 6 eV } worse. But is that 6 eV important to you? I would guess that } in practice you won't see much difference. Personally I would } rather have the big detector. } } } best regards, } mark } } Mark W. Lund, PhD } VP Engineering } } Soft X-ray Web page http://www.moxtek.com { { } MOXTEK, Inc. } 452 West 1260 North } Orem UT 84057 801-225-0930 FAX 801-221-1121 } lundm-at-xray.byu.edu } } Mike Ingram wrote: } } :I need opinions. } : } :I have an EDS system with a 30 mm premium detector which had original spec's } :of 136 eV. The crystal pack needed repair and was replaced. The resolution } :now is 142 eV. I need to decide a course of action. Here are my options. } : } :1. Keep the repaired 30 mm detector at 142 eV and be back up and } :running. } :2. Here the vendor replace the crystal pack until it meets the 136 eV } :spec, which will take time and I might be down for a while. } :3. Go to a 10 mm detector that I am told they can insure a resolution } :of 136 or better. } : } :Question for the group, if I can live with the down time should I hold out } :for the 30 mm detector at 136eV? } : } :Michael Ingram } :Rodel, Inc. } :451 Bellevue RD } :Newark, DE 19713 } :Phone: 302.366.0500, ext. 2545 } :Fax: 302.455.1124
I have an old JEOL JAMP-30 Auger Microprobe, and the monitor for the PDP 11/70 microcomputer (the computer that runs the auger software) has just died. The monitor is a Nissho Electronics Pictus 80I. Does anyone have a replacement for sale (or donation) or any idea where I might find one? Any assistance is greatly appreciated.
Brian M. Robin Research Assistant Analytical Instrumentation Facility North Carolina State University
The Alberta Research Council -Vegreville site has a Hitachi H600 TEM (with STEM attachment and Kevex 700 microanalysis system) available for the cost of moving it from our site to yours. The TEM requires a new high tension cable to be functional.
If anyone is interested in the entire unit (or maybe just parts of it) please contact either Rosemary Harris (rosemary-at-arc.ab.ca or telephone 780-632-8451) or Arlene Oatway (arlene-at-arc.ab.ca or telephone 780-632-8454).
Vegreville is located about 60 miles east of Edmonton in Alberta, Canada.
How you store it depends on your ultimate goal. If you're not going to do immunolabeling, store it in glut/paraform. These don't fix some structures well and are somewhat reversible; thus, storing in buffer for long times afterward could leach out contents. It would be even better if you could process tissue through osmium and then store in buffer.
If you're going to do IEM, don't store tissue long periods in fix, unless you know that your antigen survives this treatment. Some do; some don't.
Sara E. Miller, Ph. D. P. O. Box 3020 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-8735
We have a Kevex 8000 EDS spectrometer we do not need. The detector is a three year old thin window Quantum detector. The analyzer is probably ten years old. The Kevex detector has been horizontally mounted on an Amray 1000A scanning electron microscope, with which we are parting.
In addition, we have an Edax 9100 EDS system we do not need.
We are open to offers on both the Kevex 8000 system and the Edax 9100 system. If someone wanted both the Kevex 8000 system and the Amray 1000A SEM, that could be arranged.
I have nine un-opened 1.5 GB rewritable optical disks (double-sided) that I will never use:
Panasonic # LM-R1500A
Originally purchased for use in an Optical Access International optical disk drive.
If anyone is certain they can use these disks, please contact me.
Peter Guthrie Department of Neurobiology & Anatomy University of Utah School of Medicine 50 N Medical Drive Salt Lake City, UT 84132 (801) 581-8336 (801) 581-4233 fax Peter.Guthrie-at-hsc.utah.edu
Hello, My company is interested in obtaining an EDAX system for our JEOL T220A SEM. As this system is used on a limited basis, purchasing an entirely new system is not cost effective. Can anyone suggest resources for finding used equipment, or know of any directly? Thank you in advance.
Dysktra (spelling? this is off the top of my head) in his EM text showed micrographs of monkey kidney that had been in Trump's fix for 4 years and didn't look all that bad. (Trump's is basically the same as what you're using, slightly different %s of glut & formaldehyde.) I'd keep the specimens in fix in the frig.
Phil
} Hello every one } Here is a quandary for you } If you HAD to keep tissue (in this case 50 micron vibratome sections) at } either the primary fixation step (2% PF and 2% Glut), or in a buffer at 4 } degrees C after the primary fix. Which would be the most stable over } several weeks time? Lets assume they will be looking for general } ultrastructure. } I know there are lots of variables but I'm looking for an "in general" } answer. } } I get request from people to do EM but I can not get their sample processed } into resin at the time, so here I have a sample to store or in other cases } they want to store it until they know if they will need the EM. } } In the above experiment, another "EM" investigator told them to store the } sample in a phosphate buffer at 4 degree. Due to the number of samples I } won't be able to process them all at once so I'm not sure whether to leave } them the way they are or transfer them back into fix. I personally think } it's best to leave the sample in the primary fix at 4 degree. In the Path } EM lab we used to keep samples this way, at room temp, and the tissue } showed no discernible loss of ultrastructure even when stored a month or } two. Thanks for your advice. } } Rick Vaughn } RLVAUGHN-at-UNMC.EDU
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Dear All, Greetings of the day. Last year I had registered to attempt the MSA certification exam. Logistical problem of taking the exam from outside the US prevented me, hence, I could not take it in the previous cycles. Could any of you provide me with the new dates for the upcomming two cycles of this exam. Then again, I shall follow it up with the chair of the exam and the businessoffice of the MSA. Thanks
Mohammed Yousuf Dept. of Zoology King Saud University POB 2455 Riyadh 11451 Saudi Arabia
Hi everyone, If you know anybody that could be interested by the following job offer, please forward this email. Thanks Cecile
ps : don't sent any enquiries to me , send them to Professor J.S. Abell or Dr. T.W. Button School of Metallurgy and Materials University of Birmingham Birmingham B15 2TT
School of Metallurgy and Materials in Collaboration with Oxford Instruments Plc Research Fellowship in the Development of Superconducting Coated Tapes Applications are invited for a post-doctoral research position to work on the fabrication and characterisation of bi-axially textured YBCO films on metallic substrates. The post is supported by funding from one of the leading international industrial companies involved in the development of practical superconducting wires and tapes. The successful candidate will join an established interdisciplinary research group at the University, but will benefit from close interaction with the company both in the UK and US. Some experience of thick or thin film technology of HTC materials would be an advantage, with expertise in pulsed laser deposition being particularly attractive. The appointment will be for two years initially. Interested candidates should have a PhD in materials science or related subject.
The vacancy will be advertised formally in a short time, but informal enquiries should be sent to
Professor J.S. Abell or Dr. T.W. Button School of Metallurgy and Materials University of Birmingham Birmingham B15 2TT
J.S. Abell-at-bham.ac.uk ( 0121 414 5168 ) T.W. Button-at-bham.ac.uk ( 0121 414 5237 ) ****************************** Dr. Cecile Prouteau School of Metallurgy & Materials The University of Birmingham Edgbaston, Birmingham B15 2TT UK E-mail : c.prouteau-at-bham.ac.uk tel : (UK=44)- (0)121 414 5170 fax : (UK=44)- (0)121 414 5232
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I saw the post on LR white and noticed the no OsO4 part of the protocol. I'm having problems with some LR white just now and I'm wondering if its because the tissue (brown fat in this case) was Osmicated. The block seems hard enough except where the tissue is and around there its like rubbery butter. Interestingly enough the non osmicated blocks seem fine if maybe a little too hard. Any ideas out there?
Rod Nicholls Head Technician EM Unit University of Ottawa
Rick, its not a quandary for me. Microscopists unwilling to put in any additional effort to assure good EM results - if that course was to be subsequently chosen, pay later for their omissions. The rules are simple: For best results, tissues should be post-fixed in osmium as soon as possible. Fully fixed tissues may be stored in the cold in either buffer or a low, say 50% alcohol.
Sabatini advocated that GA fixed tissue could be held for six months in buffer and then postfixed, but only some tissues will give good results after that delayed fixation. Similarly, tissues stored in GA (forget storing in osmium!), will continue crosslinking and at high powers show a more granular texture. Sjostrand thought that he could avoid this false granularity completely, by fixing single cells for under a minute in GA. He had hoped to retain cellular details to a resolution well below 1nm. This is ancient stuff now and you may not obtain true details at such resolution from tissue sections, but storing tissues in fixative is not a good idea. Cheers Jim Darley ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com Great microscopy catalogue, 500 Links, MSDS, User Notes www.proscitech.com
On Tuesday, September 21, 1999 12:52 AM, "rlvaughn-at-unmc.edu"-at-sparc5.microscopy.com [SMTP:"rlvaughn-at-unmc.edu"-at-sparc5.microscopy.com] wrote: } } Hello every one } Here is a quandary for you } If you HAD to keep tissue (in this case 50 micron vibratome sections) at } either the primary fixation step (2% PF and 2% Glut), or in a buffer at 4 } degrees C after the primary fix. Which would be the most stable over } several weeks time? Lets assume they will be looking for general } ultrastructure. } I know there are lots of variables but I'm looking for an "in general" } answer. } } I get request from people to do EM but I can not get their sample processed } into resin at the time, so here I have a sample to store or in other cases } they want to store it until they know if they will need the EM. } } In the above experiment, another "EM" investigator told them to store the } sample in a phosphate buffer at 4 degree. Due to the number of samples I } won't be able to process them all at once so I'm not sure whether to leave } them the way they are or transfer them back into fix. I personally think } it's best to leave the sample in the primary fix at 4 degree. In the Path } EM lab we used to keep samples this way, at room temp, and the tissue } showed no discernible loss of ultrastructure even when stored a month or } two. Thanks for your advice. } } Rick Vaughn } RLVAUGHN-at-UNMC.EDU }
I am not sure if this is the right place, but maybe somebody can help.... A colleague of mine send this to me since I operate our EM's, but before I run a serious search:
"We do laser marking of anodised aluminum, and have a problem. Some mark extremely well and other samples mark extremely poorly. I need to find out the reason for this, and have a number of things I would like to test :
1) the difference between different aluminum alloys 2) the difference between anodised thicknesses 3) the difference between anodised colors 4) the effect of surface finish.
Can you help with any of these ...or suggest some technique do to measure the anodised thickness etc etc. "
Any help would be much appreciated.
Many thanks Sara
Sara Prins CSIR-National Metrology Laboratory PO Box 395 Pretoria South Africa Tel +27 12 841 3974 Cell +27 83 2972643 Fax +27 12 841 2131 e-mail sprins-at-csir.co.za http://nml.csir.co.za
:I need opinions. : :I have an EDS system with a 30 mm premium detector which had original spec's :of 136 eV. The crystal pack needed repair and was replaced. The resolution :now is 142 eV. I need to decide a course of action.} }
You probably paid more to have a 136 detector, 142's are pretty much off the reject pile IMHO.
We have also been having trouble with LR White, specifically with embedding plant cells and tissue. We have tried dehydrating though 70% ethanol, then through 100%. We have increased infiltration times, played with embedding times and temperatures, and tried different bottles of resin. We get sections that fall apart in the boats and resembled tattered napkins on the grids. The tissues and cells have been processed for immunolabeling and the labeling is working perfectly on the surviving portions of the sections, but the sections themselves look awful.
Our other resins are all working fine. So we join the ranks of the puzzled ones on this question. Any help would be appreciated.
Randy Tindall EM Specialist Electron Microscopy Core Facility W122 Veterinary Medicine Bldg. University of Missouri Columbia, MO 65211 (573)882-8304 http://www.biotech.missouri.edu/emc/
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } I am rather surprised this a.m. to arrive at the lab to find that the } } blocks I've embedded in LR White are not polymerized. This is not a } } technique we routinely use. I followed the schedule for EM ICC, tissues } } are .25-.5mm, no OSO4, ETOH dehydration to 95%, several infiltration } } changes over hours and overnight, oven calibrated to 50 degrees, gelatin } } capsules totally filled and capped, in the oven since Friday. } } Help -- What's going wrong?? What to do now ?? } } Linda Fox } } lfox1-at-wpo.it.luc.edu } } I saw the post on LR white and noticed the no OsO4 part of the protocol. } I'm having problems with some LR white just now and I'm wondering if its } because the tissue (brown fat in this case) was Osmicated. The block seems } hard enough except where the tissue is and around there its like rubbery } butter. Interestingly enough the non osmicated blocks seem fine if maybe a } little too hard. Any ideas out there? } } Rod Nicholls } Head Technician } EM Unit } University of Ottawa } } } } Hi,
Osmicated fat or even poorly osmicated fat interferes with polymerization of epoxies. This became absolutely clear to me when I tried to embed some M and Ms for paperweights. The shell cracked, the cocoa butter ran out and the area around it stayed gooey. (I had to design a special formulation which would embed M and Ms) It is possible that the same applies to the acrylics. I have done a lot of studying of Acrylics and have never seen anywhere that fat does interfere. Try dehydration to 100% and see if there is any improvement. Are you infiltrating with vigor? A number of changes, etc. Another possibility is, of course, that the fat takes up large quantities of osmium which forms a barrier to infiltration. This we see regularly in nerve bundles both in epoxies and acrylics.
If the problem does not solve itself, let me know, and I can give you some places where you might get help.
Hildy Crowley Sr. Electron Microscopist University of Denver {hcrowley-at-du.edu}
What kind of elements are typically present in your samples? If you don't routinely analyze for Cd, In, Sn and Sb, or for Pb and Bi, I think you could get along quite well with your resolution at 142 eV. The primary need for the improved resolution would be for resolving peak overlaps, although it may also provide a slight improvement in detectable limit.
Hopefully you can get some adjustment to the repair price for not restoring original performance.
Warren
At 09:29 AM 9/20/1999 -0400, "Ingram, Mike" wrote: } } I need opinions. } } I have an EDS system with a 30 mm premium detector which had original spec's } of 136 eV. The crystal pack needed repair and was replaced. The resolution } now is 142 eV. I need to decide a course of action. Here are my options. } } 1. Keep the repaired 30 mm detector at 142 eV and be back up and } running. } 2. Here the vendor replace the crystal pack until it meets the 136 eV } spec, which will take time and I might be down for a while. } 3. Go to a 10 mm detector that I am told they can insure a resolution } of 136 or better. } } Question for the group, if I can live with the down time should I hold out } for the 30 mm detector at 136eV? } } Michael Ingram } Rodel, Inc. } 451 Bellevue RD } Newark, DE 19713 } Phone: 302.366.0500, ext. 2545 } Fax: 302.455.1124
Thanks again!! The problem may be that there was no catalyst added to the resin...(one should always ask the obvious questions when novices are about ... Thank you) I re-read all the proceedures and nowhere does it state to add the benz. perox. to the resin. I assumed that the b.p. was needed for the cold cure method, where excess heat might be generated, so I did not add any. I reinfiltrated the tissues with another bolttle of resin, from EMS, catalyst added by manufacturer, and returned them to the oven. We will see!! Thanks for all the tips. Many called for higher temps. for polymerization. I am wondering if this will hinder immuno work? I will be persuing this technique for one scientist over the next few months and would appreciate all the tips, tricks and protocols you can spare. Thanks, Linda Fox lfox1-at-wpo.it.luc.edu Fax: 1-708-216-3676
I'm wondering if vapor osmication will work on dry sections. I have sections stained for immuno gold and the protocol calls for grids to be floating on pbs during the vapor fix. I'm wondering if I need to have them wet and then have to wash them or can they be done dry and thus save the sections from the rigors of washing and pbs floating. Thanks in advance.
Rod Nicholls Head Technician EM Unit University of Ottawa
Does any information or publication exist that describes or defines the "typical" variability or range that may be observed between analysts for particle counting techniques using LM? Thanks.
Robb Calabro Pharmaceutical Scientist AstraZeneca Pharmaceutical Westborough, MA USA
Did you mix in the benzoyl peroxide catalyst? really thoroughly? it takes a while to mix in properly. I needed LR White in a hurry once and ordered it from Sigma and found out to my dismay that Sigma supplies LR White without the b.p.
To Rod Nicholls: The literature I have says osmium is not compatible (causes focal points of heat).
Rod Nicholls wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } I am rather surprised this a.m. to arrive at the lab to find that the } } blocks I've embedded in LR White are not polymerized. This is not a } } technique we routinely use. I followed the schedule for EM ICC, tissues } } are .25-.5mm, no OSO4, ETOH dehydration to 95%, several infiltration } } changes over hours and overnight, oven calibrated to 50 degrees, gelatin } } capsules totally filled and capped, in the oven since Friday. } } Help -- What's going wrong?? What to do now ?? } } Linda Fox } } lfox1-at-wpo.it.luc.edu } } I saw the post on LR white and noticed the no OsO4 part of the protocol. } I'm having problems with some LR white just now and I'm wondering if its } because the tissue (brown fat in this case) was Osmicated. The block seems } hard enough except where the tissue is and around there its like rubbery } butter. Interestingly enough the non osmicated blocks seem fine if maybe a } little too hard. Any ideas out there? } } Rod Nicholls } Head Technician } EM Unit } University of Ottawa
-- Sonia Cawsey McGowan Copley Library, University of San Diego email: scawsey-at-acusd.edu home page: http://www.acusd.edu/~scawsey
I have noticed similar problems with various biolog. tissues and especially with many carbon materials like charcoal, coke, activated carbons, and others. I have always attributed this problem to the oxygen interference issue in the polymerisation of many of the acrylics. It has been my thought that the "carbon" materials and some tissues have enough absorbed O2, or similar chemistry is going on, such that it inhibits LR White polymerisation at the localized interface of the tissue. This is when I give up and use epoxy based embedding (usually Spurrs low viscosity or similar). Brad Huggins BPAmoco EM Lab Naperville, IL
} ---------- } From: Rod Nicholls[SMTP:nicholls-at-uottawa.ca] } Sent: Tuesday, September 21, 1999 10:02 AM } To: microscopy-at-Sparc5.Microscopy.Com } Subject: Re: LR White polymerization } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } } ------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } } -----------------------------------------------------------------------. } } } } } } I am rather surprised this a.m. to arrive at the lab to find that the } } blocks I've embedded in LR White are not polymerized. This is not a } } technique we routinely use. I followed the schedule for EM ICC, tissues } } are .25-.5mm, no OSO4, ETOH dehydration to 95%, several infiltration } } changes over hours and overnight, oven calibrated to 50 degrees, gelatin } } capsules totally filled and capped, in the oven since Friday. } } Help -- What's going wrong?? What to do now ?? } } Linda Fox } } lfox1-at-wpo.it.luc.edu } } I saw the post on LR white and noticed the no OsO4 part of the protocol. } I'm having problems with some LR white just now and I'm wondering if its } because the tissue (brown fat in this case) was Osmicated. The block seems } hard enough except where the tissue is and around there its like rubbery } butter. Interestingly enough the non osmicated blocks seem fine if maybe a } little too hard. Any ideas out there? } } Rod Nicholls } Head Technician } EM Unit } University of Ottawa } } }
I am learning to using DTSA to analyse EDX spectra collected with a Link Premium 136eV Si(Li) detector with a super atmospheric thin window. The detector is attached to a JEOL 2010F TEM and has a 30mm2 active area.
I would like to generate simulated spectra within DTSA and compare them with actual spectra. The idea is to determine values for the detector's physical parameters that allow's reasonably good agreement between generated and real spectra.
The problem is I have no idea of the approximate layer thicknesses and compositions appropriate for my detector. I would appreciate learning from DTSA users with similar detectors what parameters they are using. I could then treat these values as a starting point for fine tuning my own values.
I look forward to your replies. Cheers,
Mark Blackford TEM Group Materials Division, ANSTO PMB 1, Menai, N.S.W. Australia 2234
Phone 61 2 9717 3027 Fax 61 2 9543 7179
Disclaimer: The views expressed in this E-mail message do not necessarily represent the official views of ANSTO from which this message was conveyed.
Good question. We used to use the 30 mm wafer detector in the SEM because we thought we needed it for extra surface area in x-ray mapping. We now use 10 mm in one SEM and it works fine for our applications. We still have a 30 mm in the system on our 420T (TEM), to maximize counts. If it is TEM or FESEM, there might be some significant benefit to the larger surface. I also will be interested in the opinion of the experts on this one. Brad Huggins BPAmoco
} ---------- } From: Giles, Bill[SMTP:William.Giles-at-TIMET.com] } Sent: Tuesday, September 21, 1999 10:24 AM } To: microscopy-at-Sparc5.Microscopy.Com } Subject: RE: EDS Question } } ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } { {Mike Ingram wrote: } } :I need opinions. } : } :I have an EDS system with a 30 mm premium detector which had original } spec's } :of 136 eV. The crystal pack needed repair and was replaced. The } resolution } :now is 142 eV. I need to decide a course of action.} } } } You probably paid more to have a 136 detector, 142's are pretty much off } the } reject pile IMHO. } } } Bill } TIMET }
Only thing I can tell here is that oxigen is toxic to the resin. If you leave it open to air, it will not polymerize. I always overfill gelatin capsule to exclude as much air as possible. It is messy, but I have not found a clean way yet.
Increase infiltration time would not help much as the resin penetrate rather quicker into tissue. Even though the data sheet claims that you can stop at 70% alcohol step. I found you need always dehydrate more than that. Here is what I do after osmication with nervous tissue of 1-3mm size:
dehydrate 50, 70, 95, 100X2, 10 minutes each step
1:1 LR white with 100% Alcohol for 30 minutes on a rotator
Leave in pure LS white for 3hrs to overnight (for convenience only)
Put tissue in fresh LR white in a gelatin capsule. Fill as much as you can and cover it up. Make sure you close it tightly. It is always messy step.
Polymerize at 50oC over for at least 24 hrs. I have not done it for a while now. But I believe if you stop it by taking it out of oven. Then it will not polymerize any more. Put it back to oven will not help.
If no osmication is required, try UV polymerization.
LR white is alway britle and difficult to deal with. You need section a bit thicker than normally do with other resin (e.g. Epon ). I never go below 75nm. Add a bit of grease (YES!) to the boat will help to keep the section together. Use tiny wood picker, touch your "oily face". Dip it into boat. This is enough to keep the sections together.
I am not sure if this helps. But that is how I did it.
Xuejun
On Tue, 21 Sep 1999, Tindall, Randy D. wrote:
} ------------------------------------------------------------------------ } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html } -----------------------------------------------------------------------. } } } Hi, } } We have also been having trouble with LR White, specifically with embedding } plant cells and tissue. We have tried dehydrating though 70% ethanol, then } through 100%. We have increased infiltration times, played with embedding } times and temperatures, and tried different bottles of resin. We get } sections that fall apart in the boats and resembled tattered napkins on the } grids. The tissues and cells have been processed for immunolabeling and the } labeling is working perfectly on the surviving portions of the sections, but } the sections themselves look awful. } } Our other resins are all working fine. So we join the ranks of the puzzled } ones on this question. Any help would be appreciated. } } Randy Tindall } EM Specialist } Electron Microscopy Core Facility } W122 Veterinary Medicine Bldg. } University of Missouri } Columbia, MO 65211 } (573)882-8304 } http://www.biotech.missouri.edu/emc/ } } }
*********************************************************************** Xuejun Sun, Ph.D. Dept. of Experimental Oncology Cross Cancer Institute 11560 University Ave. Edmonton Alberta T6G 1Z2, Canada
Yes Rod, The instructions advise not to use osmium and our extensive online application notes also make that point. Anyway, what is the point of using osmium with LR White. That resin is well suited to immuno and cytochem processing, but these reactions are "killed" by osmium. Cheers Jim Darley
ProSciTech Microscopy PLUS PO Box 111, Thuringowa QLD 4817 Australia Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com Great microscopy catalogue, 500 Links, MSDS, User Notes www.proscitech.com
} } } } I am rather surprised this a.m. to arrive at the lab to find that the } } blocks I've embedded in LR White are not polymerized. This is not a } } technique we routinely use. I followed the schedule for EM ICC, tissues } } are .25-.5mm, no OSO4, ETOH dehydration to 95%, several infiltration } } changes over hours and overnight, oven calibrated to 50 degrees, gelatin } } capsules totally filled and capped, in the oven since Friday. } } Help -- What's going wrong?? What to do now ?? } } Linda Fox } } lfox1-at-wpo.it.luc.edu } } I saw the post on LR white and noticed the no OsO4 part of the protocol. } I'm having problems with some LR white just now and I'm wondering if its } because the tissue (brown fat in this case) was Osmicated. The block seems } hard enough except where the tissue is and around there its like rubbery } butter. Interestingly enough the non osmicated blocks seem fine if maybe a } little too hard. Any ideas out there? } } Rod Nicholls } Head Technician } EM Unit } University of Ottawa } }
Although established as a tool in materials science and physics, scanning probe microscopy (SPM) is at the beginning of its application in biomaterials science. On 2 April 1998 the first workshop entitled "Scanning Probe Microscopy in Biomaterials Science, Dentistry and Medicine" was held in Bristol, UK. What was planned to be a small workshop evolved to be an international conference with high calibre delegates and speakers from all over the world. Encouraged by the success of the meeting and supported by international academics and industrial researchers we are organising a 2nd conference.
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---------------------- Manuela Finke University of Bristol Department of Oral and Dental Science Dental Materials and Biomaterials Group Lower Maudlin Street Bristol BS1 2LY tel:0117/9284537 fax:0117/9284780 e-mail:M.Finke-at-bris.ac.uk
} I'm wondering if vapor osmication will work on dry sections. I have } sections stained for immuno gold and the protocol calls for grids to be } floating on pbs during the vapor fix. I'm wondering if I need to have them } wet and then have to wash them or can they be done dry and thus save the } sections from the rigors of washing and pbs floating. Thanks in advance. } } Rod Nicholls } Head Technician } EM Unit } University of Ottawa
I have successfully both reosmicated sections that had the Os chemically removed and osmicated sections of various polymer blends using the vapor phase. I mount the dry grids on a glass microscope slide place it in a glass Petri dish and add a ml or so of 2% OsO4/H2O to the dish bottom (not touching the grids) put the top on and let it sit for some period of time (I usually figure an hour or so, but have never tested the rate). Do this under an approved fume hood.
Good luck
John Heckman TEM Specialist MSU Center for Electron Optics
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I have used OsO4 vapour fixation, both on floating and dry grids and have found that adequate results are achieved either way. If your resin is slightly hydrophilic (e.g. LR White), then, floating the grids on aqueous OsO4 will produce better results without causing widespread deposition, of Os around the gold particles.
} } } I'm wondering if vapor osmication will work on dry sections. I have } sections stained for immuno gold and the protocol calls for grids to be } floating on pbs during the vapor fix. I'm wondering if I need to have them } wet and then have to wash them or can they be done dry and thus save the } sections from the rigors of washing and pbs floating. Thanks in advance. } } Rod Nicholls } Head Technician } EM Unit } University of Ottawa } } }
Regards,
Andrew Ochalski, Microscopy Technician, Dept. of Biology, University of Ottawa Room 108, Gendron Bldg. 130 Louis Pasteur, Ottawa, Ontario CANADA K1N 6N5 613-562-5800 x6343 FAX: 613-562 5486
I'd be willing to bet that the omission of accelerator is the cause of your trouble. Usually, I used temperatures of about 60C for 48-72 hr ,in the absence of oxygen (important), to achieve polymerisation.. Best results for immunohistochemistry were achieved using UV polymerisation at 0-4C for 24-48 hrs, though others have attributed loss of antigenicity to the UV. I have been able to achieve polymerisation of the resin using osmicated tissue, and my understanding is that heat generated locally around the Os deposits interferes with antigenicity rather than with resin polymerisation. For IHC work, aqueous uranyl acetate produces some increase in contrast, particularly of cell membranes, which are tough to see in the absence of osmium. Some respondents have mentioned sectioning problems. LR White is tough to section! I concur with others who advised REALLY infiltrating the tissue. Dehydration to 100% ethanol seemed to help, followed by LR White:EtOH (1:1) 1-3 hrs (depending on block size) LRW:EtOH (3:1) 1-3hrs Then, three changes of 100% LRW over 24 hrs at 4C before embedding. Having said all this, the rather poor appearance of non-osmicated , LR White-embedded sections led us to move away from IHC on embedded sections, to using pre- embeddibg methods whenever possible (more often than the literature might lead one to believe). } Thanks again!! The problem may be that there was no catalyst added } to the resin...(one should always ask the obvious questions when } novices are about ... Thank you) I re-read all the proceedures and } nowhere does it state to add the benz. perox. to the resin. I assumed } that the b.p. was needed for the cold cure method, where excess heat might } be generated, so I did not add any. I reinfiltrated the tissues with } another bolttle of resin, from EMS, catalyst added by manufacturer, and } returned them to the oven. We will see!! Thanks for all the tips. Many } called for higher temps. for polymerization. I am wondering if this will } hinder immuno work? I will be persuing this technique for one scientist } over the next few months and would appreciate all the tips, tricks and } protocols you can spare. Thanks, Linda Fox lfox1-at-wpo.it.luc.edu Fax: } 1-708-216-3676 }
Regards,
Andrew Ochalski, Microscopy Technician, Dept. of Biology, University of Ottawa Room 108, Gendron Bldg. 130 Louis Pasteur, Ottawa, Ontario CANADA K1N 6N5 613-562-5800 x6343 FAX: 613-562 5486
For many years we have taught the practical aspects of SEM & TEM to post-graduate students to enable them to undertake their EM projects. We are now considering the possibility of instituting a 12 week module (1 lecture + 1 prac per week) on electron microscopy (history, development, optics, vacuum, sample prep etc) We'll probably only just touch on EDX in this particular module. The students are likely to be 3rd year Science (including Geology)/Health Science undergrads most of whom have come from a technically disadvantaged background. I'm sure there are many out there who have/are successfully teaching such a module. I would like to make contact with you to get information on how you structured your course.
Thanks in advance
Mike Gregory Professor Mike Gregory Electron Microscope Unit, University of Durban-Westville Private Bag X54001 Durban, Natal, South Africa 4000
rod, i have used osmium vapour on dry lrwhite immuno-gold labbeled sections of plant tissue prior to uranyl and lead citrate staining. 30 mins vapour exposeure gave me good staining of the cell wall. vapour exposeure was carried out in chamber designed by owen& vesely vol.20/6 proceedings rms november 1985.
for preparing support films on grids we use Pioloform instead of Formvar because it is said that Pioloform support films are little more stable. When we use Grids mesh size 200 we have no problems. Now, for grids mesh size 100 our procedure does not work. The film always breaks. Does anybody know any tricks for preparing support films for grids with large mesh size?
-- Dr. Anne Heller, AG Elektronenmikroskopie, Institut f=FCr Botanik (210), Universit=E4t Hohenheim, Garbenstra=DFe 30 D-70593 Stuttgart
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