In message {Megw.230326-at-hermes} "Mount, Richard" writes: } } {Text_1} } Dennis Shubitowski asked "Has anyone successfully "unstuck" } Polaroid Type 55 negatives that have stuck together while } drying?"
{text 2}
} Films stuck emulsion to film back seem to separate easier than those } stuck emulsion to emulsion. } } ------------------------------------------------------------ } Richard J. Mount Phone: 416-813-6551 } Auditory Science Laboratory } Department of Otolaryngology FAX: 416-813-5036 } The Hospital for Sick Children } 555 University Ave. } Toronto, ON, Canada M5G 1X8 } } e-mail:richard.mount-at-mailhub.sickkids.on.ca
I agree with Richard, above, that film back stuck to emulsion is easier to seperate, and this is the case I usually run into as I dry my Polaroid negs in racks with the emulsion of one facing the back of the next neg.
A point that I left out in my previous note describing successful use of overnight water soak to seperate stuck negs is how I set this up. As with many techniques, success lies in the details of the method. I load a pair of stuck negs into my usual neg drying rack with edges of one neg in one set of slots, edges of other neg in adjacent set of slots. The effect is to separate the negs where they are not stuck to enable water to get directly to the stuck area. Success depends on getting good water soak and softening of the film emulsion. If you were to simple lay the two stuck negs flat in a pan of water, might not get as good a soak as when the films are seperated a bit to allow water access.
I hope this helps.
--
Gib Ahlstrand, MMS Newsletter Editor Electron Optical Facility, University of Minnesota, Dept. Plant Pathology 495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249 612-625-9728 FAX, giba-at-puccini.crl.umn.edu
"MICROSCOPY & MICROANALYSIS 96" in Minneapolis, Minnesota, Aug.11-15, 1996
} a user brought me a sample of gelatinous unicellular algae she } preserved in 2% phosphate buffered glutaraldehyde. Unfortunately, it was a } marine sample, so when she fixed it, the salts in the seawater } precipitated. She can't repeat the experiment. Any way to salvage her } sample--i.e., separate the cells from the ppt, or get the salts back into } soln without detroying the cells? We appreciate that the precipitation may } have ruined her cell preservation as well, but we won't know for sure till } we can embed and section. } Dear Steve, I am not an expert in this, but FWIW, I would try rinsing in buf- ferred saline using a lowish concentration of a buffer such as tris or acetate. This might wash off or redissolve the external ppts. I wouldn't think anything would get ppt out of the interiors of the cells but still preserve the morphology. I'd pay attention to the osmolarity of the rinse, although the 2% PO4+glut probably swelled the cells if they came directly from seawater. Good luck. Yours, Bill Tivol
-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --
On Feb. 29, the following question was asked:
} Subject: Mo aperture grids } } I am looking for TEM grids with single hole of 0.8, 1.0 and 1.5 mm in } diameter and slot of 1x2 and 0.5x2 mm. I tried several vender and did not } succeed. Anybody know a source of these grids? } We have had in production Mo support "rings" for use during the ion milling of samples for quite some time. They are 3mm diameter OD with an ID of 1.5 mm. Thickness is 0.5 mm. These should work out just fine for you.
You can find them either on page 74 of the current SPI Supplies SourceBook or else in our electronic on-line catalog at the ULR given below. They will be found on the "page" with the ultrasonic disc cutter.
Chuck
====================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President 1-(800)-2424-SPI SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: GVKM07A-at-prodigy.com West Chester, PA 19381-0656 USA Customer Service: SpiSupp-at-aol.com
Workshop Objective This course will cover all aspects of pre-thinning and focus on final thinning via Tripod Polishing. Due to the limited class size and the extensive hands-on opportuinities, this course is well suited to novices as well as advanced Tripodders. The course will include sections on:
How to do it and why should I? What's really going on and what am I really seeing? How to prepare small, specific area cross-sections. The problem of wildly differing materials (eg tungsten). Rapid preparation of TEM cross-sections. Preparation of a wide range of materials: semiconductors, ceramics, metals,...
Hands-on Opportunity This course will be unique in that it will provide a hands-on opportunity for every class participant. Tripod Polishers, Polishing Wheels, and pre-thinning equipment will be made available to all participants and actual samples will be prepared - by the students - as part of the course. This is a great opportunity to get your hands dirty and actually learn by doing. The instructors will walk you through each step of the process and then let you loose on the equipment. This course is designed to teach the Tripod Polishing technique. Silicon samples will be provided to the students and used as the basis for the course teaching.
Workshop Location and Dates South Bay Technology - San Clemente, CA Dates: Friday & Saturday - June 14-15, 1996 Friday & Saturday - October 18-19, 1996
Previous Participants (partial list) INTEL, AMD, Motorola, LSI Logic, Conner Peripherals, Univ of Maryland, Univ of New Mexico, UNAM (Mexico), LG Electronics (Korea), Battelle, MEMC, MVA Inc., Univ of Michigan, U.S. Bureau of Mines, IBM, Naval Research Lab, Purdue Univ, Univ of Alabama, Univ of Arizona, Univ of Colorado, Univ of Wisconsin.
Class Size Due to the intensive hands-on aspects of this course, class size will be strictly limited to 10 participants.
Registration Fee: $795 (includes lunches and Friday night Dinner) $695 if registration fee paid by April 30, 1996 for June Workshop and by August 31, 1996 for October Workshop
Registration Deadline: 30 days prior to workshop
For additional Information: Monica Pflaster South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673 TEL: 800-728-2233 FAX: 714-492-1499 e-mail: sbt-at-msa.microscopy.com
Registration Form
To register for the workshop, please fill out this form and send it, with registration fee to:
South Bay Technology, Inc. Workshop on Tripod Polishing 1120 Via Callejon San Clemente, CA 92673
Payment must be made in the form of a check, money order, Visa or MasterCard. Checks must be drawn on a U.S. Bank and made payable to South Bay Technology, Inc. Credit card orders by FAX may be sent to South Bay Technology at 714-492-1499.
I am looking for software (Macintosh) for SAED indexing. Does anyone know a source of such software? Thanks for your help. Yours sincerely, Liuying Wei
A coworker of mine wanted to know if there was anything to etch fossils that have been altered to iron oxide.
Thanks in advance, Peling Melville
-------------------------------------------------------------- Peling Fong Melville Senior Scientific Assistant Interdepartmental Facilities American Museum of Natural History Central Park West at 79th Street New York, NY 10024-5192 U.S.A. ****************************** E-mail: peling-at-amnh.org Work #: (212) 769-5469 FAX #: (212) 769-5495
A private non-profit foundation [401(3)(C) status] is looking for a used ultramicrotome and glass knifemaker through a donation or inexpensive purchase.
If you can help, please contact Dr. Gary Sibert at (505) 434-1725.
I am a microscopist in TEM Laboratory of the University of New Mexico. I hope to get some new information from you every day. Could you send me some infromation every day if possible?
Does anyone know if adding sodium azide to a plant cell suspension culture (final concentration 0.02%) affect its ultrastructure?
It was added 30 minutes before fixing the cells in 2.5% glutaraldehyde. Azide was used in order to stop cell activity at a specific time point. The main interest in the ultrastructure analysis is to look at the Golgi apparatus, cell wall, plasma membrane, and endoplasmic reticulum.
If it does (or doesn't) affect the ultrastructure; How? Which organelles are affected the most? Are there references in the literature about it?
Thanks in advance for your input.
Manrique Rojas Page Owen Department of Botany Connecticut College
I am sending this on behalf of a customer who does not have e-mail access.
He would like to communicate with anyone who has a Tracor TN5500 analyser for sale. The customer is in Southern Ontario (Canada), so would only consider units in Canadian or USA due to shipping costs.
Please e-mail to me directly, and I will pass the information on to my customer.
YES, virginia, polaroid negatives can usually be unstuck, just soak in very hot water about 10 -20 minutes, then GENTLY pull them apart, GRADUALLY turning and pulling a little untilthe whole gelatine that is stuck gets soaked through and losened. Some times if more completely dried out, the emulsion of one will be stuck to the second, usually the second can be saved completely, but some of the first will be lost (natually the center, best part of the image) good luck
} Unsticking polaroid negatives } that have stuck together while drying? A water soak did not } seem to help nor did Photoflo.
} Thanks,
} Dennis Shubitowski } University of Michigan } School of Dentistry
Alan S. Pooley ,PhD Bivalve shell SEM & shape analysis SEM/Morphometrics lab Marine & Coastal Sciences Rutgers University 908 932 8959 ext 225 Pooley-at-ahab.rutgers.edu
Suggest you try Paul Fischione. He is at 412-325-5444.
Best regards,
Ellie Solit
On Thu, 29 Feb 1996, Berta, Yolande wrote:
} } Dear Microscopists, } } We have a chunk of beryllium, and we would like to know the thickness of the } oxide layer that forms on the metal within a given time interval. SEM hasn't } shown the oxide layer, so now we are thinking that maybe a TEM cross-section } specimen would be the thing to try. } } Has anyone prepared beryllium for TEM before? How? Ion milling? } Jet-polishing? How was it cut to keep the dust at a minimum? } } Yolande Berta } School of Materials Science and Engineering } 778 Atlantic Dr. } Atlanta, GA 30332-0245 } (404)894-2545 } yolande.berta-at-mse.gatech.edu }
This seems to be a perfect application for Auger electron spectroscopy which is sensitive to the first few monolayers on the surface. The surface is ion beam sputtered to determine the oxide depth. Auger spectroscopy is very sensitive to Be.
If you don't have your own instrument, or one on campu, there are several commercial laboratories providing this service, including ours.
Joe Geller Geller MicroAnalytical Lab 426e Boston St. Topsfield, MA 01983-1216 508 887-7000
On Thu, 29 Feb 1996, Berta, Yolande wrote:
} } Dear Microscopists, } } We have a chunk of beryllium, and we would like to know the thickness of the } oxide layer that forms on the metal within a given time interval. SEM hasn't } shown the oxide layer, so now we are thinking that maybe a TEM cross-section } specimen would be the thing to try. } } Has anyone prepared beryllium for TEM before? How? Ion milling? } Jet-polishing? How was it cut to keep the dust at a minimum? } } Yolande Berta } School of Materials Science and Engineering } 778 Atlantic Dr. } Atlanta, GA 30332-0245 } (404)894-2545 } yolande.berta-at-mse.gatech.edu }
Alternate-Recipient: allowed Auto-Forwarded: prohibited Content-Return: allowed Disclose-Recipients: prohibited Conversion: allowed Importance: normal Priority: normal Sensitivity: Company-Confidential
Azide inhibits the mitochondria and yes it has a profund effect on the cytoskeleton and cytoplasmic streaming in stamen hair cells of Setcresea. Read E. Tucker and N.S. Allen, 1986. Cell Motil and the cytoskel. 6:305-313.
On Fri, 1 Mar 1996, T. Page Owen Jr wrote:
} } Does anyone know if adding sodium azide to a plant cell suspension } culture (final concentration 0.02%) affect its ultrastructure? } } It was added 30 minutes before fixing the cells in 2.5% glutaraldehyde. } Azide was used in order to stop cell activity at a specific time point. } The main interest in the ultrastructure analysis is to look at the Golgi } apparatus, cell wall, plasma membrane, and endoplasmic reticulum. } } If it does (or doesn't) affect the ultrastructure; How? Which } organelles are affected the most? Are there references in the literature } about it? } } Thanks in advance for your input. } } Manrique Rojas } Page Owen } Department of Botany } Connecticut College } }
I received a posting asking about replacement bulbs for some piece of equipment. Unfortunately, before I completely digested the message, I deleted it (I guess the hand is really quicker than the eye). I have an excellent source for bulbs of all kinds, even those that are no longer manufactured. You might want to give them a call to see if they have what you are looking for. They are:
Bulbs Only 954 Queen Street Southington, CT 06489 (203) 621-0213
I might add that they are extremely helpful and ship the next day.
Martin A. Levin Department of Biology Eastern Connecticut State University Willimantic, CT 06226 Phone: (860)465-4324 FAX: (860)465-5213
On 25 Feb 1996 VFN6T-at-DMT03.mcc.Virginia.EDU wrote:
} Dear Colleagues, } I am using plastic-embedded (Lowicryl or JB-4) sections of fetal kidneys } and would like to use standard avidin-biotin-peroxidase techniques for } immunohistochemistry. To date, we are unable to achieve specific staining. } We have tried two different etching protocols and a few of the JB-4 (but none } of the Lowicryl) sections survive, but still no staining. Most references } give little information regarding incubation times, etc. Can anyone help me} trouble-shoot or point me towards a good reference? Thanks in advance. } Victoria F. Norwood, M.D. } Department of Pediatrics } University of Virginia } vfn6t-at-dmt03.mcc.virginia.edu } } Dear Victoria, A failure to immunolabel is usually due to the alteration of the antigenic conformation during tissue processing. Each antigen/antibody combination requires different conditions (fixation, incubation times, choice of resin etc.) which have to be determined by trial and error approach. Some antibodies will never label. Though some monoclonal antobodies label very nicely, chances are better with polyclonal antisera (affinity purified) as they bind to several antigenic determinants of the antigen and some might be preserved better than others. The best reference would be the one using your particular antibody. Others can give you only general directions for finding the optimal protocol (e.g. Hyatt's three volumes of Immunogold labelling -I don't have the exact reference here, sorry). First, I would try to stain paraffin sections, or even better, cryostat sections. If there is no staining there, chances are that it will not work in resin. However, if this is successful, you can use it to determine the optimal concentration and incubation time and then proceed to Lowicryl or LR White sections (I have no experience with JB4). You should not need to etch them. Some antigens, however, require enzyme digestion (trypsin or pepsin). Again this you would try on paraffin or cryosections first. You might need to experiment with fixation. For TEM, I usually start with three fixation protocols: 4% paraformaldehyde (PF), 2% PF+ 0.1 % glutarladehyde (GA) or 2% GA to find the optimal tissue preservation vs. labelling conditions. I hope this will help a bit. These methods can be very frustrating at times. You may contact me directly at my E-mail address if you need more details.
Sarka Lhotak lhotaks-at-fhs.mcmaster.ca EM Facility, McMaster University Hamilton, Ontario
We have recently been approached with an interesting analytical problem concerning the detection of trace ( {200 ppm) levels of heavy elements in animal tissue. Elements of interest include selenium,nickel,arsenic,copper and many others. The samples will be presented as thin (5 um) slices on a glass slide substrate. Most of our work involves non-biological samples and this job represents an interesting challenge. We have available a Cameca 3f SIMS to carry out this work. We plan to carry out depth profiles and also SIMS imaging. Can anyone provide some useful tips in tackling this problem? Also,is there any other techniques available that could also address this question? Any suggestions would be greatly appreciated.
Sincerely, Ross Davidson Phone: 519-661-2173 Surface Science Western FAX: 519-661-3709 Western Science Centre, Rm G1, E-mail: davidson-at-surf.ssw.uwo.ca University of Western Ontario Home Page: http://www.uwo.ca/ssw/ London, Ontario, Canada N6A 5B7
} a user brought me a sample of gelatinous unicellular algae she } preserved in 2% phosphate buffered glutaraldehyde. Unfortunately, it was a } marine sample, so when she fixed it, the salts in the seawater } precipitated.
I would suggest trying to dissolve the precipitate by diluting the buffer. The combination of phosphate buffer and seawater is clearly not a good one. In the past I always used glutaraldehyde in seawater as a primary fixative for marine algae. I suspect it's the calcium in the seawater who is to blame.
She can't repeat the experiment. Any way to salvage her } sample--i.e., separate the cells from the ppt, or get the salts back into } soln without detroying the cells? We appreciate that the precipitation may } have ruined her cell preservation as well, but we won't know for sure till } we can embed and section.
Alternatively one may keep part of the samples and proceed with embedding and sectioning as usual, without trying to remove the precipitate. Sectioning may be a bit difficult but some useful results may be possible to obtain. I wouldn't use a diamond knife in this case, though.
Yours sincerely
Dr Stephan Helfer, SSO Mycologist / Plant Pathologist
Royal Botanic Garden Edinburgh, Inverleith Row, EDINBURGH EH3 5LR, Scotland UK
http://www.rbge.org.uk
phone: +44 (0)131 552 7171 ext 280 or +44 (0)131 459 0446-280 (direct digital VoiceMail line) fax: +44 (0)131 552 0382 ============================ 1896 * BRITISH * MYCOLOGICAL * SOCIETY 1996 A century of fungal science ============================
Can someone point me to some information about how to use a hanging drop slide. This should be obvious I guess, but I am sure there are some little tricks that we would rather not have to figure out the hard way. Thanks- Dave
Dr. David Knecht Department of Molecular and Cell Biology University of Connecticut U-125 Storrs, CT 06269 Knecht-at-uconnvm.uconn.edu
Good morning, I've recently been trying to do immunolocalization at the EM level with affinity-purified Ab. My problem is that the purified Ab's are in an ammonium thiocyanate solution that chews up both the Ni grids and the sections (LR White). I know it's the solution because the polyclonal serum immunos are fine. Does anyone with experience purifying Ab's and using them for TEM immuno care to chime in with some pertinent advice? I'd appreciate hearing any and all suggestions. Many thanks!
Dwight Beebe Institut de recherche en biologie vegetale beebed-at-ere.umontreal.ca Dept. de sciences biologiques Voice:514-872-4563 Universite de Montreal FAX:514-872-8496 4101, rue Sherbrooke est Montreal, PQ H1X 2B2 Canada
Re: Immunostaining of plastic (JB-4, LRWHite) sections. According to the circular accompanying the LR White resin, avidin-biotin binding is masked by the plastic--they recommend using a PAP technique instead. The same may be true with the methacrylate resins too, so your problem could conceivably be caused by your choice of embedding medium. Needless to say, the advice to examine other aspects of the system is of course wise especially if you've never tried this specific detection using another preparation technique. I found it to be amazing what antigens can really withstand once the plastic masking problem has been solved.... Good luck. Grace Kennedy
I am trying to analyze graphite grains that are scattered in a sample for their nitrogen contents. The mass absorption coefficient for nitrogen Ka line in the carbon matrix is very very high (23,586). Moreover, the nitrogen is a minor or trace element in the graphite.
Is this an impossible task for the probe analysis ? Are there any other techniques can to used to complete this task ? Thanks.
Fellow Microscopists, We are trying to locate other analytical laboratories that might be interested in benchmarking. Specifically, we would like to benchmark types of services offered, size, education and expertise of staff, cycle-time, and SPC. We are an semiconductor facility but may find acceptable benchmarks with labs that provide .services to other than semiconductor houses. Thanks! Please reply to: Carolyn Casoria Honeywell/SSEC -at- casoria-at-ssec.honeywell.com
Due to a consolidation of two microscopy facilities, we would like to reluctantly part with the following excess instrument which is in excellent condition and under service contract:
HITACHI H800/810 200 kV TEM LaB6 electron gun Motorized goniometer stage SEM/STEM capabilities Tracor/Northern (Noran) 5402 energy dispersive X-ray microanalysis system with a 8502/S Advanced Image Analysis system Special specimen holder for X-ray analysis of sectioned specimens Special specimen holder for X-ray analysis of bulk specimens Rotation/tilt specimen holder for grids.
Shipping the instrument is buyer's responsibility.
BONUS: If the purchase is completed before May 15th, 1996, a HITACHI HHS-2R SEM (in storage) will be offered free of charge to the H800 buyer.
Please contact me directly if interested.
************************************************************************* M.V. Parthasarathy Professor of Plant Biology & Director, Cornell Integrated Microscopy Center Section of Plant Biology 228 Plant Science Building Cornell University, Ithaca, NY 14850 Telephone: (607) 255-1734 Fax: (607) 255-5407 E-mail: mvp2-at-cornell.edu ***********************************************************************
To make a hanging drop slide: You will need a depression slide, a square coverslip, some petrolatum, and the liquid suspension of what you wish to view.
Place a small spot of petrolatum on each of the 4 corners of the coverslip. Place a drop of your suspension in the center of the coverslip. Invert a depression slide over the drop, allowing the petrolatum to attach the coverslip to the depression slide. Quickly (but carefully) invert the slide so that the coverslip is oriented "up", and the drop is hanging into the slide depression.
That's all there is to it.
W. L. Steffens, Ph.D
Dept. of Veterinary Pathology College of Veterinary Medicine University of Georgia Athens, GA 30602 STEFFENS.B-at-CALC.VET.UGA.EDU Voice: (706) 542-5536 FAX: (706) 542=5828
To make a hanging drop slide: You will need a depression slide, a square coverslip, some petrolatum, and the liquid suspension of what you wish to view.
Place a small spot of petrolatum on each of the 4 corners of the coverslip. Place a drop of your suspension in the center of the coverslip. Invert a depression slide over the drop, allowing the petrolatum to attach the coverslip to the depression slide. Quickly (but carefully) invert the slide so that the coverslip is oriented "up", and the drop is hanging into the slide depression.
That's all there is to it.
W. L. Steffens, Ph.D
Dept. of Veterinary Pathology College of Veterinary Medicine University of Georgia Athens, GA 30602 STEFFENS.B-at-CALC.VET.UGA.EDU Voice: (706) 542-5536 FAX: (706) 542=5828
Message-Id: {9603042108.AA1032-at-pho903.sbphrd.com} To: microscopy {microscopy-at-Sparc5.Microscopy.Com} {Beverly_E_Maleeff-at-sbphrd.com}
Philadelphia Society for Microscopy (PSM) March Meeting
When: Tuesday, March 12, 1996
Where: Laboratory for the Research of Science and Materials (LRSM), on the campus of the University of Pennsylvania, 33rd and Walnut Sts., Philadelphia, PA
Program: 5:30 PM Social hour 6:30 PM Dinner Members $12.00, Non-members $15.00 7:30 PM Speaker
Speaker: Dr. Roberto F. Nicosia, Dept. of Pathology, Medical College of Pennsylvania and Hahnemann University Philadelphia, PA
"The Cell Biology of Blood Vessel Growth: Light microscopic, immunohistochemical and ultrastructural studies"
Reservations will be taken by Ms. Pat Overend at the University of Pennsylvania, 215/898-8337. Deadline for dinner reservations is Friday, March 8. Cancellations must be received no later than 5:00 PM, March 11, 1996.
If you have any questions regarding the meeting please contact Rollin Lakis at the University of Pennsylvania, 215/898-8718.
To make a hanging drop slide: You will need a depression slide, a square coverslip, some petrolatum, and the liquid suspension of what you wish to view.
Place a small spot of petrolatum on each of the 4 corners of the coverslip. Place a drop of your suspension in the center of the coverslip. Invert a depression slide over the drop, allowing the petrolatum to attach the coverslip to the depression slide. Quickly (but carefully) invert the slide so that the coverslip is oriented "up", and the drop is hanging into the slide depression.
That's all there is to it.
W. L. Steffens, Ph.D
Dept. of Veterinary Pathology College of Veterinary Medicine University of Georgia Athens, GA 30602 STEFFENS.B-at-CALC.VET.UGA.EDU Voice: (706) 542-5536 FAX: (706) 542=5828
I've had excellent results air drying from hexamethylsilizane at room temperature. Peldri will also work if you've got any left. Filtering through a filter works fine. But what % size change are you looking for? Recall the measuring things, especially biological specimens in the SEM is dicey at best--and can be grossly misleading. Phil Oshel
} Hi All: } } I have been asked to examine, using the SEM, some bacteria that have been } exposed to a drug solution. The interest is in whether the exposure to the } drug solution has altered the bacterial shape and size (mostly size) and } thus effect its retention on a filter. I propose to simply filter the } bacterial suspension onto a polycarbonate membrane, air dry, mount, sputter } coat, and examine. } Damian Neuberger } Email: neuberd-at-baxter.com
&&& Illigitimi non carborundum &&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&& Philip Oshel Center for Electron Microscopy University of Illinois (217)244-3145 oshel-at-ux1.cso.uiuc.edu
To make a hanging drop slide: You will need a depression slide, a square coverslip, some petrolatum, and the liquid suspension of what you wish to view.
Place a small spot of petrolatum on each of the 4 corners of the coverslip. Place a drop of your suspension in the center of the coverslip. Invert a depression slide over the drop, allowing the petrolatum to attach the coverslip to the depression slide. Quickly (but carefully) invert the slide so that the coverslip is oriented "up", and the drop is hanging into the slide depression.
That's all there is to it.
W. L. Steffens, Ph.D
Dept. of Veterinary Pathology College of Veterinary Medicine University of Georgia Athens, GA 30602 STEFFENS.B-at-CALC.VET.UGA.EDU Voice: (706) 542-5536 FAX: (706) 542=5828
To make a hanging drop slide: You will need a depression slide, a square coverslip, some petrolatum, and the liquid suspension of what you wish to view.
Place a small spot of petrolatum on each of the 4 corners of the coverslip. Place a drop of your suspension in the center of the coverslip. Invert a depression slide over the drop, allowing the petrolatum to attach the coverslip to the depression slide. Quickly (but carefully) invert the slide so that the coverslip is oriented "up", and the drop is hanging into the slide depression.
That's all there is to it.
W. L. Steffens, Ph.D
Dept. of Veterinary Pathology College of Veterinary Medicine University of Georgia Athens, GA 30602 STEFFENS.B-at-CALC.VET.UGA.EDU Voice: (706) 542-5536 FAX: (706) 542=5828
Dwight -- Try dialyzing the affi-pure Ab into Tris-Buffered Saline. I use 10mM Tris, 500mM NaCl, pH 7.2 for use on LRGold sections. Avoid phosphate as it causes lead stain pption. Good Luck,
Greg Martin Dept. of Cell Biology and Anatomy Johns Hopkins School of Medicine
On Mon, 4 Mar 1996, Dwight Beebe wrote:
} Good morning, } I've recently been trying to do immunolocalization at the EM } level with affinity-purified Ab. My problem is that the purified Ab's } are in an ammonium thiocyanate solution that chews up both the Ni grids } and the sections (LR White). I know it's the solution because the } polyclonal serum immunos are fine. Does anyone with experience purifying } Ab's and using them for TEM immuno care to chime in with some pertinent } advice? I'd appreciate hearing any and all suggestions. Many thanks! } } Dwight Beebe } Institut de recherche en biologie vegetale beebed-at-ere.umontreal.ca } Dept. de sciences biologiques Voice:514-872-4563 } Universite de Montreal FAX:514-872-8496 } 4101, rue Sherbrooke est } Montreal, PQ H1X 2B2 Canada } }
Antisera can be easily separated from undesirable salts by dialysis...this is by far the simplest method. Obtain a short length of dialysis tubing, soak it in water or buffer which will allow it to be opened up into a tube. Tie off one end, fill with your antibody solution, then tie off the other end. Immerse it into whatever buffer you want to antibody to end up in (I suggest either TRIS buffered or phosphate buffered saline. Use 1000X as much buffer as antiserum, ie dialyse 1 cc of antibody against 1 liter of water for 24 hours, then change the buffer and continue for another 24 hours. I recommend the last buffer change to contain 0.001 mM sodium azide to retard microbial growth.
This will hopefully solve your problem. W. L. Steffens, Ph.D
Dept. of Veterinary Pathology College of Veterinary Medicine University of Georgia Athens, GA 30602 STEFFENS.B-at-CALC.VET.UGA.EDU Voice: (706) 542-5536 FAX: (706) 542=5828
At 3:57 PM 3/4/96 -0800, Neuberger, Damian wrote: } Hi All: } } I have been asked to examine, using the SEM, some bacteria that have been } exposed to a drug solution. The interest is in whether the exposure to the } drug solution has altered the bacterial shape and size (mostly size) and } thus effect its retention on a filter. I propose to simply filter the } bacterial suspension onto a polycarbonate membrane, air dry, mount, sputter } coat, and examine. This will supposedly work for the Gram positive microbes } but may not work for the Gram negatives (they are said to have a much } thinner wall and could shrink). An alternate approach is to heat fix the G- } to a glass coverslip, sputter coat, and examine. } } Is this approach OK or is there a better way to do this, perhaps using a } fixative? I would rather not have to go the route of CPD. Any suggestions } will be greatly appreciated!!! } } Damian Neuberger } Email: neuberd-at-baxter.com
Dear Damian, One alternative to CPD is to filter, fix, dehydrate and follow the final 100% ETOH dehydration with 3 baths of 100% HMDS (Hexamethyldisilizane). Polycarbonate membranes handle the solvent very well. They dry in minutes and you can quickly afix them to stubs and sputter coat. Rosemary WAlsh, EM Facility, PSU
In the absence of a means to coat biological specimens with Cr for high(er) resolution SEM, we use the electron guns in our Balzers 400 freeze-fracture device to coat with various metals and carbon. Several years ago Hans Ris suggested Pt/Ir/C coating, and we have had very good results with this, although we have to constantly struggle with some of the parameters. I am interested in hearing from others who use this technique to see if we can compare notes. For example, should the coating be done cold or at room temperature? We have had some success under both conditions. What kinds of distances, angles, vacuums, etc.?
On a related note, we had been purchasing 80% Pt/20% Ir wire and balling it up and melting it into drilled out C rods (supplied by Bal-Tec), but this was a pain to do as compared with using the Pt inserts made for the drilled rods. Bal-Tec would make the Pt/Ir pellets to order, but at substantial cost. Larry Stevens at Stevens Metallurgical Corp. has agreed to make the Pt/Ir pellets and/or rods at a very reasonable price if there appears to be enough of an interest. I would be like to hear from others who might buy Pt/Ir pellets so that we might encourage Mr. Stevens to do so!
Aloha from sunny, warm Hawaii (and surf's up), Tina ***************************************** Tina (Weatherby) Carvalho * Biological Electron Microscope Facility * University of Hawaii * (808) 956-6251 * tina-at-ahi.pbrc.hawaii.edu * http://www.pbrc.hawaii.edu/bemf/ * *****************************************
I have been asked to examine, using the SEM, some bacteria that have been exposed to a drug solution. The interest is in whether the exposure to the drug solution has altered the bacterial shape and size (mostly size) and thus effect its retention on a filter. I propose to simply filter the bacterial suspension onto a polycarbonate membrane, air dry, mount, sputter coat, and examine. This will supposedly work for the Gram positive microbes but may not work for the Gram negatives (they are said to have a much thinner wall and could shrink). An alternate approach is to heat fix the G- to a glass coverslip, sputter coat, and examine.
Is this approach OK or is there a better way to do this, perhaps using a fixative? I would rather not have to go the route of CPD. Any suggestions will be greatly appreciated!!!
Damian Neuberger Email: neuberd-at-baxter.com
Hello Damian!
No fix and air drying will almost certainly change the shape and size of the bacteria. If you wish to avoid critical point drying you could try fixation followed by a CPD substitute such as HMDS (hexamethyldisilazane), TMS (tetramethylsilane) or Peldri (if available). Alternatively, freeze-drying, cryo-SEM or LVSEM would be preferable to air-drying without fixation.
Regards,
Robin Cross Director : EM Unit, Rhodes University, Grahamstown, South Africa eurc-at-giraffe.ru.ac.za (tel: +27 461 318168 - fax: +27 461 24377)
besides SEM and SEM-EDS analysis we are doing XRF services since not so long ago. We know this may not be the forum to asking about XRF but, we have experienced the kindness of many of you out there.
Our questions are:
* do any of you know of any listserver for X-Ray Fluorescence Spectroscopists to discuss ideas with?
* we're desperately needing mylar or polypropilene film for doing XRF analysis, do you know a source for XRF supplies ? We'd appreciate very much receiving postal or e-mail addresses or fax to contact them as soon as possible.
We thank all of you for your attention,
Angel Di Giandomenico cnono-at-arcride.edu.ar
Centre for Research and Development Santa Fe - Argentina
As part of an introductory course on microscopy, I am trying to set up field trips so students can observe practical applications of acoustic and scanning tunneling microscopy.
So ... I am looking for industrial, commercial or academic labs within driving distance of Williamstown, MA that would be willing to spend some time with a small group of eager undergraduates. Dates to be determined.
} To: "Neuberger, Damian" {neuberd-at-engrnd.roundlake.baxter.com} } From: gwe-at-biotech.ufl.edu (Greg Erdos) } Subject: Re: Air Dried Bacteria? } Cc: } Bcc: } X-Attachments: } } } } } Hi All: } } } } I have been asked to examine, using the SEM, some bacteria that have been } } exposed to a drug solution. The interest is in whether the exposure to the } } drug solution has altered the bacterial shape and size (mostly size) and } } thus effect its retention on a filter. I propose to simply filter the } } bacterial suspension onto a polycarbonate membrane, air dry, mount, sputter } } coat, and examine. This will supposedly work for the Gram positive microbes } } but may not work for the Gram negatives (they are said to have a much } } thinner wall and could shrink). An alternate approach is to heat fix the G- } } to a glass coverslip, sputter coat, and examine. } } } } Is this approach OK or is there a better way to do this, perhaps using a } } fixative? I would rather not have to go the route of CPD. Any suggestions } } will be greatly appreciated!!! } } } } Damian Neuberger } } Email: neuberd-at-baxter.com } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } Get your bacteria to attach to a polylysine coated cover slip. Fix with glut and osmium, dehydrate and dry via HMDS (two changes of hexamethyldisilazane after 100% alcohol, then air dry finishing up with ten minutes in a 60 C oven). Then sputter coat Will give you much better results than heat fixing and air drying. } -at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at- Greg Erdos Phone: 352-392-1295 Scientific Director, ICBR Electron Microscopy Core Lab 218 Carr Hall Fax: 352-846-0251 University of Florida E-mail: gwe-at-biotech.ufl.edu Gainesville, FL 32611 -at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
WANT TO BUY OR ACCEPT DONATION: electron microscope
Usable JEOL JEM-100C, 100CX, 100S , or equivalent. Must be in OK running condition. Accessories, high resolution, etc. unimportant. Will pay for shipping. This is for my colleague studying polymer materials. You may contact me if you have one to give away or have any lead. Thank you in advance.
Tung Hsu Material Science Center National Tsing-hua University Hsin-chu 30043 TAIWAN
Recently, I sent a message to you all about a used TEM, to see if there was any interest. I have had some, so here is the real offer.
We have a Philips 201c TEM for sale, it is approximately 20 years old. It is functional, but lack of use has caused the vacuum to be less than optimal. With a little TLC this scope could be an excellent workhorse. The investigators who own the scope would be willing to sell it for parts, but they want it removed from their facility, not just scavenged, as they need to make room for a new scope.
Thanks in advance
Cheri Owen Wayne State University Detroit Neurotrauma Institute Detroit, MI (313)577-4648
Recently, I sent a message to you all about a used TEM, to see if there was any interest. I have had some, so here is the real offer.
We have a Philips 201c TEM for sale, it is approximately 20 years old. It is functional, but lack of use has caused the vacuum to be less than optimal. With a little TLC this scope could be an excellent workhorse. The investigators who own the scope would be willing to sell it for parts, but they want it removed from their facility, not just scavenged, as they need to make room for a new scope.
Thanks in advance
Cheri Owen Wayne State University Detroit Neurotrauma Institute Detroit, MI (313)577-4648
Dear all, Has anyone out there prepared non-conducting ceramic fibres for TEM, either across or along axis? If so, how did you do it? We have some mullite fibres we would like to look at and although we have some ideas about how to prepare specimens we would appreciate any ideas or feedback on this topic.
Thanks
Dr. Ian MacLaren, IRC in Materials for High Telephone: 0121 414 3447 Performance Applications, FAX: 0121 414 3441 The University of Birmingham, email: I.MacLaren-at-bham.ac.uk Birmingham B15 2TT, England
Nitrogen in graphite can be detected to a concentration level of approximately 0.08% in a relatively short amount of time using the LDE type of metal crystal in a wavelength dispersive x-ray spectrometer mounted on a electron probe microanalyzer. The spatial resolution would be on the order of 1 or 2 micrometers.
If you don't have this type of instrumentation there are commercial laboratories offering this service, including ours.
Joe Geller Geller MicroAnalytical Laboratory 426e Boston ST. Topsfield, MA 01983-1216 508 887-7000 fax 887-6671 On 4 Mar 1996, Liang, Long wrote:
} Dear Microscopists, } } I am trying to analyze graphite grains that are scattered in a sample } for their nitrogen contents. The mass absorption coefficient for } nitrogen Ka line in the carbon matrix is very very high (23,586). } Moreover, the nitrogen is a minor or trace element in the graphite. } } Is this an impossible task for the probe analysis ? Are there any other } techniques can to used to complete this task ? Thanks. } } Long Liang } ARCO EPMA/SEM Lab } } }
I am looking for good reference books and lab manuals containing protocols for use in a course on histotechnique\microtechnique. Anyone have some good recent and classical books (Title, Author, Publishers)? Many thanks.
############################################################################# John J. Bozzola, Ph.D., Director Center for Electron Microscopy Southern Illinois University Carbondale, IL 62901-4402 U.S.A. Phone: 618-453-3730 Fax: 618-453-2665 Email: bozzola-at-siu.edu Web: http://www.siu.edu/departments/shops/cem.html #############################################################################
John, Best books I know of are John Kiernan's _Histological and Histochemical Methods_ and the Biological Stain Commission's _Staining Procedures_ (9th or 10th [or...?] edition). I don't have Kiernan's book to hand, but if he doesn't respond, I can send you the correct info. (Same for _Staining Procedures_.) I heard there was a good EM book with some procedures written by some guy in southern Illinois... Phil Oshel
} I am looking for good reference books and lab manuals containing protocols } for use in a course on histotechnique\microtechnique. Anyone have some good } recent and classical books (Title, Author, Publishers)? Many thanks. } } ############################################################################# } John J. Bozzola, Ph.D., Director } Center for Electron Microscopy } Southern Illinois University } Carbondale, IL 62901-4402 } U.S.A. } Phone: 618-453-3730 } Fax: 618-453-2665 } Email: bozzola-at-siu.edu } Web: http://www.siu.edu/departments/shops/cem.html } #############################################################################
&&& Illigitimi non carborundum &&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&& Philip Oshel Center for Electron Microscopy University of Illinois (217)244-3145 oshel-at-ux1.cso.uiuc.edu
-------------------------------------- Do I have to caot the grids ? Thanking you in advance for the suggestions, i am looking forward to try this technique annette Bakker
Is there an available color-spectrum illustrating the color changes undergone in silicon as it is thinned. This effect is routinely used for final polishing but I have never actually seen a quanitative correlation for just how thin 'yellow' transmitting silicon is, for example. I presume it is also orientation dependent, but don't know to what degree.
A color-guide would make a very useful HTML page for new users.
Daniel L. Callahan Department of Mechanical Engg. and Materials Science Rice University dlc-at-owlnet.rice.edu
Good morning I am looking for audio visual aids (video, CDŠ) for illustrating theory and/or possibilities of all kinds of LM. This is for use in a course on investigation methods. Recent reference books or booklets edited by manufacturers are also welcome. I'd appreciate your suggestions. Many thanks!
Prof. Jean-Pierre Auquiere Universite catholique de Louvain (UCL) Secr. Acad. Faculte des Sciences Unité de botanique generale (BOTA) Place des Sciences, 2 Place Croix du Sud, 4 B-1348 Louvain la Neuve, Belgium B-1348 Louvain la Neuve, Belgium tel + 32 10 47 86 78 tel + 32 2 764 51 22 fax + 32 10 47 28 37 fax + 32 2 764 72 55 E mail secretaire-at-sc.ucl.ac.be E mail auquiere-at-bota.ucl.ac.be
Considering that photography is still so important to our profession, it is likely that in this group are a few critical individuals with personal experiencee using dry-to-dry black and white photographic processors. Would anyone care to recommend a counter-top processor that they are happy with? I wouldn't object to knowing which ones to stay away from either.
Many thanks,
Doug Keene Shriners Hospital for Crippled Children Portland, Oregon
A useful introductory text on LM sample processing and staining methods is Animal Tissue Techniques, Gretchen L. Humason, W.H. Freeman, I believe the 3 rd edition is the most recent.
Regards,
Glen MacDonald Research Scientist Hearing Research Laboratories of the Virginia Merrill Bloedel Hearing Research Center Box 35-7923 University of Washington Seattle, WA 98195-7923 (206) 616-4156 glenmac-at-u.washington.edu
On Tue, 5 Mar 1996, John. J. Bozzola wrote:
} I am looking for good reference books and lab manuals containing protocols } for use in a course on histotechnique\microtechnique. Anyone have some good } recent and classical books (Title, Author, Publishers)? Many thanks. } } ############################################################################# } John J. Bozzola, Ph.D., Director } Center for Electron Microscopy } Southern Illinois University } Carbondale, IL 62901-4402 } U.S.A. } Phone: 618-453-3730 } Fax: 618-453-2665 } Email: bozzola-at-siu.edu } Web: http://www.siu.edu/departments/shops/cem.html } ############################################################################# } } }
If you would like to access the most up to date information about the joint meeting between MSA, MAS & MSC-SMC you can get everything on-line on the WWW.
This includes: Scientific Program Short Courses Posters Special Awards Financial Support Social Events How to submit manuscripts Registration Forms Dates, Times, Deadlines, etc...
Just access the MSA WWW site at
http://www.msa.microscopy.com
Look for the hot links to "Registration & Meeting Info - 1996s"
Cheers... Nestor
Your Friendly Neighborhood SysOp & the Microscopy & Microanalysis Program Chair for 1996
} Has anyone out there prepared non-conducting ceramic fibres for TEM, either } across or along axis? If so, how did you do it?
Dear Ian, This may or may not be applicable, but with the high-voltage EM (lower interaction cross-section) and very low beam currents, we have been able to image non- or poorly-conducting specimens without charging or heat- ing problems. If this holds true for your instrument (e.g. you have an IVEM), the advantage is that there may be no preparation required; the disadvantage is that with these low doses you will either need a very sen- sitive recording medium--LoDose or other x-ray film, image plates or in- tensified or slow-scan CCD--or long exposures. The large grain or speci- men drift could make the images useless. Good luck. Yours, Bill Tivol
California State University and the California Society For Microscopy
May 4 & 5, 1996 San Diego State University
REGISTRATION AND ABSTRACTS
The abstracts will be compiled into the "Proceedings of the Fourth Annual Microscopy Colloquium" and will be published in Microscopy Research and Technique. Abstract information and details on email submission of abstracts available on request.
Abstracts must be received by March 29,1996
Registration fees (by March 29): $35/regular, $10/student, $50/vendor
Late Registration fees (March 29): $45/regular, $10/student, and $60/vendor.
Please send the enclosed registration form whether or not you intend to make a presention
PLATFORM AND POSTER PRESENTATIONS
Papers discussing any aspects of microscopy in cell, developmental, and structural biology, or material and geological sciences are welcome. Microscopy techniques papers and student presentations are particularly encouraged. Platform presentations will be held on Saturday, May 4th and Sunday morning, May 5th. Posters will be displayed Saturday, May 4, with authors present.
PROGRAM
Friday - May 3: 4:30 p.m. Business meeting for CSU delegates at Comfort Inn, La Mesa
Saturday - May 4: 8:00 a.m. Registration/Aztec Hall San Diego State University 9:00 a.m. Opening remarks 9:15 a.m. Platform presentations 10:30 a.m. Coffee break 10:45 a.m. Platform presentations 12:00 p.m. Buffet lunch 1:30 p.m. Platform presentations 3:00 p.m. Coffee break 3:15 p.m Poster presentations 4:00 p.m. Poster authors present 4:45 p.m. Reception/ Banana Quad Tour of the SDSU EM Facility 6:00 p.m. Banquet 7:30 p.m. Keynote address
Presentation: O Platform O Poster O No Presentation
Meal Preference: O Chicken O Fish O Vegetarian
Make checks payable to "EM Facility/Colloquium"
SEND TO:
Dr. Steven Barlow EM Facility/Biology Dept. San Diego State University 5500 Campanile Drive San Diego, CA. 92182-4614
(619) 594- 4523 sbarlow-at-sunstroke.sdsu.edu
HOUSING:
The Comfort Inn, La Mesa
2 miles from SDSU
Housing for the Colloquium will be available at the Comfort Inn, 8000 Parkway Drive, La Mesa. The room rate is $42.00 per night, plus tax. You must make your own reservations. Please call (619) 698-7747 and specify that you will be attending the Microscopy Colloquium.
Shuttle Service:
Airport shuttle service is available from several companies. We recommend the California Sunshine Shuttle Service at $12 per person to SDSU and the Comfort Inn. Please make advanced reservations by calling collect (619) 443-7900, 6 a.m. to 9 p.m. PST and specify that you will be attending the Microscopy Colloquium. A free shuttle will provide limited tranport between SDSU and the Comfort Inn Saturday and Sunday.
Dr. Steven Barlow EM Facility/Biology Dept. San Diego State University San Diego, CA 92182-4614 phone: (619)594-4523 fax:(619)594-5676 email:sbarlow-at-sunstroke.sdsu.edu
Alternate-Recipient: allowed Auto-Forwarded: prohibited Content-Return: allowed Disclose-Recipients: prohibited Conversion: allowed Importance: normal Priority: normal Sensitivity: Company-Confidential "Childress, Charles P. (Chip)" {cpc4-at-NIOBBS1.EM.CDC.GOV} , daughecc-at-UCBEH.SAN.UC.EDU, LLOYDPF-at-ml.wpafb.af.mil Message-Id: {960306140912.626-at-cliff.ml.wpafb.af.mil.0}
Thanks to all who have responded speedily to my enquiry about preparation of ceramic fibres for TEM. I will be trying some of these ideas in the next few days.
Thanks again
Ian MacLaren, IRC in Materials for High Telephone: 0121 414 3447 Performance Applications, FAX: 0121 414 3441 The University of Birmingham, email: I.MacLaren-at-bham.ac.uk Birmingham B15 2TT, England
} Considering that photography is still so important to our profession, it is } likely that in this group are a few critical individuals with personal } experiencee using dry-to-dry black and white photographic processors. Would } anyone care to recommend a counter-top processor that they are happy with? I } wouldn't object to knowing which ones to stay away from either. } } Many thanks, } } Doug Keene } Shriners Hospital for Crippled Children } Portland, Oregon } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } We have been using the Ilford 2150 RC for over a year, maybe two by now. The results are quite good, however there have been mechanical problems with it from the get go. We had to replace bearings early on and have had to dismantle and re-assemble the rollers over and over to keep it from squeaking and sqealing and grinding. So it has been a mixed bag for us. We do about 10,000 prints a year and it has been a real time-saver too -at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at- Greg Erdos Phone: 352-392-1295 Scientific Director, ICBR Electron Microscopy Core Lab 218 Carr Hall Fax: 352-846-0251 University of Florida E-mail: gwe-at-biotech.ufl.edu Gainesville, FL 32611 -at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Does anyone know who published, and the current source, of a series of tutorial papers called "Microscopy and Photomicrography"? They were written by Stanley Klosevych of the University of Ottawa.
Fellow microscopists, what is the best and most efficient way to get rid of an old TEM?? I see many for sale, etc., but have never seen replies. We have an old Hitachi H-500 STEM, maintained by Hitachi until last year when the $ ran dry. It's been used strictly as a biological TEM for the last 7 years or so. I would hardly thnk it would pay anyone to have it disassembled and shipped, and it can't be left in place. Thanks in advance for any input.
The good people at the MSA office will be glad to help. Ask for Sharon or Larry. Their number is 800-538-3672
See you at MSA.
Ellie Solit The Microscope Book
On Wed, 6 Mar 1996, Lee Wagstaff wrote:
} Hi Gang, } } Anyone out there know where to obtain some comprehensive info on the MSA meeting coming } up this August?? Any assistance would be appreciated. Thanks. Lee. }
The best processor out there is the Mohrpro. We have been using one for about 8 years and recommend it when ever we can. It is easy to use and maintain, 2 min. dry to dry, permanent (not stabilized) and uses RC paper and Kodak Fixer. We do purchase the developer from Mohr. The current cost for paper up to 8" width is $4295, the Mohrpro8. and 14" width $4800, the Mohrpro14. (They do sell rebuilt machines as well). They stand behind their product and I can only say great things about Mohr. For information contact: Bob or Jim Jackson (tell them Linda at Loyola sent you!) Mohr Enterprises 65 E. Palatine Rd. Suite 103 Prospect Heights, Il 60070 1-847-465-0048
Linda Fox Loyola University Medical School lfox1-at-wpo.it.luc.edu
WHAT: The Spring meeting of the Northern California Society for Microscopy.
WHEN: Thursday, March 14 from 5:30 to 9:30 p.m.
WHERE: Genentech, 460 Point San Bruno Blvd., So. San Francisco, Ca
Speakers: Dr. Wen Lu Li, Genentech -
"Microscopy as a Tool in the Development of Theraputic Molecules" & Dr. Ming Pan, Gatan -
"High Resolution Transmission Electron Microscopy of Zeolites"
The Northern California Society for Microscopy (NCSM) welcomes new members. Anyone interested in microscopy of any type, from atomic force to optical will benefit from joining the NCSM. We meet four times a year to have dinner and hear about new developments in microscopy. Dues are very reasonable at only $15 a year for regular members, and $8 for students. If you are interested in joining, or knowing more about NCSM, please contact Kent McDonald by phone at 510-642-2085 during normal business hours, or by email at: klm-at-uclink4.berkeley.edu If you are interested in attending the Mar. 14 meeting described above, please call 510-486-4088 no later than Mar. 8 so we can reserve the appropriate number of dinners. Complimentary drinks will be provided by MicroStar Diamond Knives and RMC and the dinner will be $15 for members, $7.50 for student members. Please join us.
What about museums and such? I know that there are technology/medicine museums scattered about which might have a use. I know that the National Museum of Health and Medicine here at the AFIP has a microscope collection, and I believe the Smithsonian might as well. I assume that there would be other museums in the country which would also benefit from such donations.
billo
On Thu, 7 Mar 1996, grace kennedy wrote:
} Fellow microscopists, what is the best and most efficient way to get rid of } an old TEM?? I see many for sale, etc., but have never seen replies. We } have an old Hitachi H-500 STEM, maintained by Hitachi until last year when } the $ ran dry. It's been used strictly as a biological TEM for the last 7 } years or so. I would hardly thnk it would pay anyone to have it } disassembled and shipped, and it can't be left in place. Thanks in advance } for any input. } } }
Hello All, The Scanning 96 Meeting in Monterey, CA on 9-12 April is looking for students to monitor the sessions, ie. run the slide projectors, adjust lights and hand out information. The student will have the registration fee waived, in appreciation of helping. For information, please contact me, or the Scanning 96 office at FAMS, Inc., PO Box 832, Mahwah, NJ 07430-0832. E-mail: fams-at-holonet.net Thank you, Debe Holmberg USDA-ARS 916.752.9021 916.752-4604 (fax)
Si color changes related to us by Tom Cass in '81 when VCR GROUP designed the first DIMPLER, D100 were:
Garnet10-12um, Wheaty brown 7-9um, yellow 4-6um, white 1-3um, fringes {1um
The basic dimpler design came from his lab at Hewlett Packard Deer Creek Labs, EMSA Bullentin 10(1) 1980, p.66, Addendum 10(2) 1980, p.93.
The colors depend on the intensity and type of lighting, Si dimpled and viewers color perception. Specimen thickness can be measured quickly and accurately on the DIMPLER specimen platen with a good optical microscope. This measurement technique is especially helpful with specimens that are not light transmitting.
I have a Zeiss Universal 'M' microscope which is missing a few parts, which are now VERY expensive (from Zeiss).
I require a REFLECTOR for a 'Vertical Illuminator IIC'; either a H-PL, or H-PR will do. Only the reflector is required as I have the housing. Does anyone have one of these ? Or does anyone know if it is possible to 'make' one using a 'double sided semi-transparent mirror' ?
Many thanks
steve
============================================================================== Steve Schwarz sschwarz-at-morgan.ucs.mun.ca Dept. of Earth Sciences Memorial University of Newfoundland Newfoundland CANADA A1B 3X5 1-709-737-8142 -737-2589 FAX ******************************************************************************
} Fellow microscopists, what is the best and most efficient way to get rid of } an old TEM?? I see many for sale, etc., but have never seen replies. We } have an old Hitachi H-500 STEM, maintained by Hitachi until last year when } the $ ran dry. It's been used strictly as a biological TEM for the last 7 } years or so. I would hardly thnk it would pay anyone to have it } disassembled and shipped, and it can't be left in place. Thanks in advance } for any input.
Metal recyclers are an option. Taking parts home as unusual potplant holders (bits of the column), paperweights (the lead glass), decorations etc is fun. Perhaps a local school would like it.
Diana van Driel Dept Ophthalmology C09 Sydney University 2006 NSW, AUSTRALIA
} Does anyone know who published, and the current source, of a series } tutorial papers called "Microscopy and Photomicrography"? They were } written by Stanley Klosevych of the University of Ottawa. } } Thank you in advance, } David Rothbard
Dear David,
I believe these tutorials have been published as a single volume, but I do not know the details of that.
They were also published as a series of six papers in the Journal of Biological Photography in 1974 and 1975. The details are:
Part I vol. 42:part 3, pp 123-131 Part II 42:4, 147-160 Part III 43:1, 30-38 Part IV 43:2, 51-69 Part V 43:3, 119-139 Conclusion 43:4, 187-188
About 24 degrees Celsius and not a cloud in the sky. Who needs Hawaii?
Regards
Stephen Edgar
Electron Microscope Unit, Pathology Department School of Medicine University of Auckland Private Bag 92019 Auckland New Zealand
Announcement of Workshop/Symposium -------------------------------------------
The National Center for Electron Microscopy at the Ernest Orlando Lawrence National Laboratory will be hosting a 3 day workshop on Quantitative High Resolution Transmission Electron Microscopy, April 18 - April 20, 1996. This workshop is designed to address many important issues relating to obtaining quantitative information from experimental data in HRTEM. The workshop will begin with discussing the many steps required in order to ensure meaningful quantitative information, starting with the recording of the experimental data, transforming recorded information into digital form, correcting for anomalies of the recording media and processing of the data. Procedures for obtaining quantitative measurements of important parameters such as chemical composition, grain boundary expansion/contraction, strain and lattice parameters will be outlined, including a discussion of the accuracy of the measurements. The workshop will cover problems associated with matching of calculated images
to experimental data and address the possibility of creating standards for reporting goodness of fit between experimental and simulated images and confidence levels for refined structural models. There will be one day devoted to reconstruction methods in HRTEM including a discussion on the limits of current reconstruction methods.
Registration for the meeting is $150. Total number of participants is limited to 40. Application for registration can be obtained by contacting Gretchen Hermes (see contact information below) or by mailing or faxing (to Gretchen Hermes) the application form obtainable from the web-site listed below.
For information on registration and further details on the workshop, please see the web site: http://ncem.lbl.gov/NCEM/workshops.html -------------------------------------------------- Roar Kilaas NCEM/MSD, MS 72-207 Lawrence Berkeley National Laboratory 1 Cyclotron Road, Berkeley, CA 94720 tel: 510-486-4618 fax: 510-486-5888 email: r_kilaas-at-lbl.gov
} Does anyone know who published, and the current source, of a series of } tutorial papers called "Microscopy and Photomicrography"? They were } written by Stanley Klosevych of the University of Ottawa. Dear David, The Stanley Klosevych series was published in the Microscopical Society of Canada Bulletin. The Bulletin Editor, Caroline Emerson (email: cemerson-at-kean.ucs.mun.ca), would be the best place to try for copies. I don't remember exactly when the series was published. There is also a very good book by him: "Principles and Practice of Microscopy and Scientific Photography", which can be ordered on the MSC home page:http://gause.biology.ualberta.ca/craig/hp/MSC.SMC.Mic.Soc.hp. or you can link from the MSA/Microscopy site. Luck, Mary
Mary Mager Electron Microscopist Metals and Materials Eng, UBC 309 - 6350 Stores Rd. Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648, fax: 604-822-3619 email: mager-at-unixg.ubc.ca
Microscopy of Surfaces and Interfaces Midwest Microscopy and Microanalysis Society Workshop UWM Microscopy Open House
April 26, 1996
Please register by April 15, 1996 More information and online registration at http://www.uwm.edu/Dept/LSS/meeting.html
PROGRAM Golda Meir Library Conference Center University of Wisconsin - Milwaukee
Opening Ceremonies 8:45 - 9:00
Morning Session
9:00 - 9:30 J. Mansfield University of Michigan - Ann Arbor "Materials Science and Biological Applications of Environmental Scanning Electron Microscopy"
9:30-10:00 B. Tonner University of Wisconsin - Milwaukee "X-ray Microscopy for Chemical Analysis of Surfaces"
10:10 - 10:15 Coffee Break and Mounting of Posters Sponsored by College of Letters and Sciences
10:15 - 10:45 T. Kelly University of Wisconsin - Madison "Three-Dimensional Atom Probe Microscopy"
10:45-11:15 J. Nogami University of Wisconsin - Milwaukee STM: "Studies of Metal Growth on Silicon Surfaces"
11:15-11:45 L. D. Marks Northwestern University - Evanstone Surface HREM: "Old techniques on Old Surfaces - Surprising New Results"
Open House Lunch
11:45 - 1:00 Lunch sponsored by Nissei Sangyo America and Hitachi Scientific Instruments. Poster Session
1:00 - 1:45 Poster viewing and poster competition Poster prizes are sponsored by the MMMS.
Afternoon Session
1:45-2:15 J. M. Gibson University of Illinois - Urbana TEM: "All you ever wanted to know about building your own expensive and complicated microscope, and then waiting five years until it finally works"
2:15 - 2:45 S. Babcock University of Wisconsin - Madison TEM/HREM: "Electron Microscopy Studies of Grain Boundaries in Bicrystals"
2:45 - 3:15 V. Dravid Northwestern University - Evanstone "Analytical Electron Microscopy and Holography of Interfaces: Making a Mountain out of a Mole Hill"
3:15 - 3:30 Coffee Break and Judging of Posters Sponsored by Laboratory for Surface Studies
3:30 - 4:00 N. Browning University of Illinois at Chicago "High Angle Annular Dark Field in a Dedicated STEM"
4:00 - 4:30 K.L. Merkle Argonne National Laboratory "Relaxation Modes in Metal and Oxide Grain Boundaries"
4:30- 4:45 Poster Awards Ceremony and Closing Address M. Gajdardziska-Josifovska, Workshop Organizer
Open House Tour
REGISTRATION FEES: Registration is free for the members of the Midwest Microscopy and Microanalysis Society. The workshop registration fees for non-members are same as the membership fees and can be paid at the conference site (by check or cash): Regular Member ($10) Student Member ($5)
POSTERS All lectures are by invitation only. Poster contributions by students, researchers and microscope manufacturers are welcome. There are no size restrictions for the posters, however, they should be mounted on a sturdy backing suitable for display on easels. The poster must be relevant for the broad topic of microscopy of surfaces and interfaces.
POSTER COMPETITION
Graduate students are particularly encouraged to present their work and they are eligible to enter a poster competition. One grand prize and two first prizes will be given by the Midwest Microscopy and Microanalysis Society. The grand prize must be used by the winner to attend a microscopy related session at a major national/international conference.
Grand Prize: $500 1st Biological Sciences:$100 1st Physical Sciences: $100
We have just disposed of an old microscope - a HITACHI HU-11E.
After advertising it on the Net and finding no takers we dismantled it. We kept things like DP and RP pumps, transformers, motors and guages but found the recovery of scrap metal very limited. The chassis fetched the princely sum of R33.00 from the local scrap dealer. Perhaps as a teaching facility the greatest value was the fact that we now have a wonderful assortment of dissected lenses, aperture holders, electron gun etc to use as teaching material.
I don't believe that it is a kindness to donate a 27 year old fragile valve-powered instrument such as this to a school or suchlike, the maintenance responsibilities outside of expensive, specialised hands are overwhelming.
An enquiry to the Japanese suppliers gave us a one-liner which we have already framed - "Please throw away TEM as scrap metals"
Sobering moment seeing the frame of our beloved ex-TEM swinging from a crane in the scrapyard before being dumped onto a pile of old oil drums........
Tony Bruton Centre for Electron Microscopy University of Natal Pietermaritzburg KwaZulu Natal South Africa
I am using a Philips CM30ST for high resolution imaging. I have had a problem performing the critical objective lens direct alignment "current rotation center". Ideally, one should obtain a stationary point on the specimen, rotating about its center at high magnification with a low-frequency wobble applied to the objective lens current. I can get the stationary point on the specimen reasonable satisfactorily. The probjem is that the beam moves fairly significantly when the objective lens wobbler is on.
I have performed the coma free direct alignment, which should ensure that the beam is travelling along the optic axis of the objective lens. This works well. However, it seems to me that if wobbling the objective lens current can shift the beam, it can't be travelling the optic axis.
Is there any relatively quick way to correct the problem of beam shift with objective lens current change? I can imagine that a complete realignment of the microscope might get rid of the problem. However, this is both very time-consuming and also not very instructive as to the cause of the problem.
Dear all, I have received a whole bunch of suggestions about the above subject and someone has asked me if I would share a summary of the responses with the whole list, this is given below. Thanks again to all who responded. We have decided to embed the fibres in epoxy as suggested by some of the contributors, dimple and ion mill (at a low angle). I don't think that we have a tripod polisher but I will be checking that out.
____________________________________________________________________________ My original message:
Dear all, Has anyone out there prepared non-conducting ceramic fibres for TEM, either across or along axis? If so, how did you do it? We have some mullite fibres we would like to look at and although we have some ideas about how to prepare specimens we would appreciate any ideas or feedback on this topic. ____________________________________________________________________________ We have been routinely preparing ceramics fiber tows (Nextel, Nicalon, HPZ, etc.) with and without ceramic coatings for TEM characterization for about two years now. After much trial and error with a number of methods (some in the literature) we have settled on a rather efficient method of producing high-quality sections. We are just finishing a short communication on this technique, which I could send to you when completed.
First, the tows are vacuum-impregnated with a high-temperature epoxy, such as G-1. It is important that the fiber-volume fraction be high, which can be achieved by either pulling many tows through the tip of a pipette, or alternatively using shrink tubing. The epoxy is then allowed to cure.
The key to obtaining a good thin section with a large electron transparent area is to minimize ion-milling time, which requires mechanically thinning the sample to thicknesses less than 5 um, ideally less than 1 um. We use a tripod polisher with 3M diamond lapping films to minimize differential polishing and surface relief. The epoxy-impregnated tows have been thinned both axially and transversely; either way wil produce good specimens. Again, you want to thin the specimen as much as possible prior to ion milling; if the specimen is too thick the epoxy will be milled away before the fibers and any coating becomes electron transparent, with the fibers usually falling out. We also need the epoxy to bound the coating for coating thickness determination.
The specimen is then mounted on a Cu washer, and maybe a grid, and ion milled at low angles for 30-60 min.
For more details please contact me off line.
Dr. Michael Cinibulk cinibumk-at-ml.wpafb.af.mil Wright Laboratory/MLLM Wright-Patterson Air Force Base, Ohio ____________________________________________________________________________ Do you have a tripod polisher in your prep lab.
We have a way of prepping fibers using a tripod that worked nicely for cross and longitudinal sections of SiC fibers.
Ron Anderson ____________________________________________________________________________ I assume that you are aware of the difficulties in doing this. I know of two methods which work very well and are based on other, relatively well-known techniques for producing cross-sections.
A) embed in resin and ultramicrotome cross-sections; this is only convenient if you have someone who does ultramicrotomy already, in which case it is probably the simplest.
B) sandwich with epoxy between two silicon wafers and then cross-section 'normally,' just like a VLSI device; by visually monitoring optical transmission through the silicon, you can thin the composite structure well below 10 micrometers before ion-milling (which in turn helps mitigate differential milling effects). This works for almost everything. The same basic technique could be used to thin the fiber along the axis.
Please let me know if something easier comes along, since I have to be doing this as well...
Daniel L. Callahan Assistant Professor of Materials Science Rice University http://www.owlnet.rice.edu:80/~dlc/ ____________________________________________________________________________ I had pretty good luck preparing ceramic fibers (mullite, SiC, Al2O3/ZrO, and others), of 10 to 30 micron diameter, for TEM analysis. The method is very simple, and consists of first making a composite out of the fibers and an epoxy, then thining down as with a bulk sample (polishing, dimpling, ion milling). Things to keep in mind:
1. If the fibers are short and tangled as a small cotton ball, saturate the epoxy with the fibers mixing very well. The fibers will brake but that doesn't matter since is unlikely that you will be interested in crystalline defects. Squeeze the composite between two teflon or other non-sticking sheets using glass slides for backings.
If the fibers are long (more than 3mm) you can lay down a thin layer of epoxy on a teflon sheet, and the fibers on top, one at a time or in bundles, in a single direction to cover a strip 3mm wide x length of the fibers. Then squeeze between two glass slides and another teflon sheet covered with epoxy. Cure the epoxy as per manufacturer instructions. In both cases you will be able to form a very thin composite that requires a little grinding and polishing on both sides before punching out 3mm discs, which can be further polish to 100 micron. Dimple as usual (with diamond paste)
2. Epoxy: try with whatever you have available. Some people prefer conductive epoxies. Initially, I used several conductive and non conductive products from TRA-CON (Medford, MA, 508-391-5550), but for some time now I have settled on G1 epoxy from GATAN (412-776-5260).
3. Ion Milling: the mullite I worked with milled very fast probably because it was very porous. Other ceramics mill very slowly compared to the epoxy. This is a problem that can be alleviated using a LN2 stage in the ion mill, to slow down the milling rate of the epoxy, and having as much fiber content as possible in the mixture.
4. Even if you use a conductive epoxy you may have to carbon coat the thinned samples.
Good Luck!
Augusto Morrone Univ. of Florida Materials Science and Engineering P.O.Box 116400 (352) 392-6985 amorr-at-mse.ufl.edu ____________________________________________________________________________ been using the Tripod polisher and a unique technique for tightly binding fibers together. They have been working with SiC fibers, but I think that it would work for you. Their Email addresses are CINIBUMK-at-ml.wpafb.af.mil and SCHELTFJ-at-ml.wpafb.af.mil
- -Scott Walck ____________________________________________________________________________ I think that someone here used a Gatan PIMS to do this several years ago with some success. The length of the fiber went across the 3mm washer. John Hunt ____________________________________________________________________________ Over the years I've prepared various fibre materials in various ways. These all should give results; the quality varies, but then so does the effort required.
1) If the fibres are thin to begin with, pop them onto a support film covered grid, carbon coat, and voila!
2) If you have experience with ultramicrotomy, embed and section small areas.
You may require a coupling agent to promote resin adhesion, and the result of sectioning will be a mass of shattered fragments (unless the fibres are amorphous), but then you might also get a huge amount of viewable area free of chemical contamination.
3) Mix the fibres into a particularly hard resin such as Petropoxy which you can then prepare (cut, grind, dimple, ion mill or tripod) as a monolithic lump. I'm not aware of any resin hard enough to thin at the same rate as a ceramic, but I've prepared SiC fibres this way with success.
4) Create a composite by electroless deposition of metal (usually Ni) which you can then prepare in the ion mill. This one gives by far the best result in my experience, but is also the trickiest and most potentially time consumming. A short electroless plate to make the fibres conductive , followed by electrolytic deposition in standard plating baths is a good option if you have access to such. The method I've used was adapted from metal powder prep. techniques.
Let me know if you'd like further information on any of this.
############################################################### # # # Don Steele STEELE-at-KRDC.INT.ALCAN.CA # # ALCAN INTERNATIONAL # # Kingston Research and Development Center # # P. O. Box 8400 # # Kingston, Ontario Canada K7L 5L9 # # # ############################################################### ____________________________________________________________________________ The answer is simple (at least in principle!) - embed them in a hard epoxy resin or acrylic, then section them in an ultramicrotome, collect on fine-meshed grids, pop in the TEM, and ---presto, nice uniformly thin fiber cross-sections held together by the resin. There will undoubtedly be some tearing of the section and/or individual cross-sections. The fibers will be randomly dispersed so that you'll get many orientations (inlcuding some near-longitudinal slices, if they're useful). Be careful of beam effects - most embedding materials are somewhat to terribly beam sensitive.
Sound too easy to be true? You're right, ultramicrotomy is somewhat of an art, but can accomplish wonders in materials science. A good reference is an overview by yours truly:
T.F. Malis and D. Steele, Specimen Preparation for Transmission Electron Microscopy of Materials, MRS vol 199, Materials Research Society (1990) p.3.
If you don't have any MRS Proceedings there, I have a few copies left and can send one, but only if you're seriously interested, as they're in short supply. The people at UMIST in the Corrosion Center are experts in this area as well. I tend to act as a information bank for this technique, so let me know how you fare - I'm always looking for new references to quote.
Tom Malis Group Leader - Materials Characterization Materials Technology Laboratory Natural Resources Canada (Govt. of Canada) Ottawa, Ontario ph.: 613-992-2310 FAX: 613-992-2310 e-mail: tom.malis-at-cc2smtp.emr.ca ____________________________________________________________________________ This may or may not be applicable, but with the high-voltage EM (lower interaction cross-section) and very low beam currents, we have been able to image non- or poorly-conducting specimens without charging or heat- ing problems. If this holds true for your instrument (e.g. you have an IVEM), the advantage is that there may be no preparation required; the disadvantage is that with these low doses you will either need a very sen- sitive recording medium--LoDose or other x-ray film, image plates or in- tensified or slow-scan CCD--or long exposures. The large grain or speci- men drift could make the images useless. Good luck. Yours, Bill Tivol ____________________________________________________________________________ I am a little late in responding to your inquiry on ceramic fibers; My apologies. Since some of the other responses mentioned using the wedge technique for preparing thin TEM sections, I wanted to mention that BUEHLER KKB, with whom I am affiliated, is a supplier of a wedge polishing tool such as those mentioned. I previously was Applications Engineer for South Bay Technology who offered the first commercially available tripod based polisher. However, I have since come to work for BUEHLER, LTD in the US, and we have made some changes to the IBM design in order to enhance the ease of producing wedge samples. We also offer a complete line of diamond lapping films for this polishing system.
If you would be interested in more information regarding BUEHLER's MICROPRECISE(TM) Tripoint Polisher, I would be happy to have literature sent to you, and/or have a salesperson contact you. If you would like more information, please feel free to contact me directly by phone, fax or e-mail.
Best regards, Scott D. Holt BUEHLER, LTD. 41 Waukegan Rd. Lake Bluff, IL 60044 USA Phone: (847)295-4546 Fax: (847)295-7942 102467.2752-at-compuserve.com ____________________________________________________________________________
Ian MacLaren, IRC in Materials for High Telephone: 0121 414 3447 Performance Applications, FAX: 0121 414 3441 The University of Birmingham, email: I.MacLaren-at-bham.ac.uk Birmingham B15 2TT, England
Coma-free alignment (stationary image of amorphous carbon when applying plus/minus tilts at high magnification, 500-700K) and current centering (specimen stationary when oscillating the lens current) are completely different. The reason is that the top and bottom parts of the objective lens are never completely lined up, the mechanical tolerances are too severe. Coma-free alignment finds the optic axis in a true sense from the sample downwards taking into account the misalignment of the two parts of the objective lens. The beam is still likely to shift when the objective lens current is oscillated since this is largely determined by the top part of the objective lens. As a test, you can measure the difference in tilt between: a) Coma-free alignment b) Current-center alignment c) HV-center (oscillating the voltage, equivalent to testing the alignment of all the post specimen lenses) d) The tilt such that the beam does not translate when the objective lens current is oscillated.
By comparing d) and a) you can get a measure of the misalignment between the top and bottom parts of the polepiece; comparing a), b) and c) you can check if the other post specimen lenses are well aligned. If the difference between d) and a) is large then you have a bad lens, and you will have problems getting small probes for microanalysis without artifacts (e.g. tails).
} Is it necessary to get a 30 mm window for an EDS detector to be used on a } Schottky emitter FE-SEM in order to get enough counts, or is a 10 mm okay? } } Thanks in advance, } Melanie Behrens } Texaco, Inc. } Fuels and Lubes Research Dept. } Beacon, NY } behrema -at- Texaco.com or MelanieOwl -at- aol.com
Our 10mm does fine, as long as the apts and lens are set properly.
} an old TEM?? I see many for sale, etc., but have never seen replies. We } have an old Hitachi H-500 STEM, maintained by Hitachi until last year when } the $ ran dry. It's been used strictly as a biological TEM for the last 7 } years or so. I would hardly thnk it would pay anyone to have it } disassembled and shipped, and it can't be left in place. Thanks in advance } for any input.
Do you have an art department? Some sculpture students are inspired by old machine parts and they usually handle the shipping quickly. About 3 trips for a Volkswagon bus (They were rated as half ton vehicles).
Good Luck Jim
Jim Romanow Electron Microscopy Facility Physiology and Neurobiogy Department The University Of Connecticut Storrs bsgphy3-at-uconnvm.uconn.edu
Alternate-Recipient: allowed Auto-Forwarded: prohibited Content-Return: allowed Disclose-Recipients: prohibited Conversion: allowed Importance: normal Priority: normal Sensitivity: Company-Confidential
On the subject, a friend uses the old vacuum pump from a Phillips for the vacuum oven he uses for embedding in parafin and epoxy. I don't know if it is qualitatively better thant a typical house vacuum line, but our house vacuum has been known to be unreliable.
Dear Doug: We've had our Mohr Pro8 for about 9 months and love it. This model came highly recommended by other users in the Boston area who had used them much longer than us. We now wonder how we put up with the tray method for so long! If you want more info, E-mail or call me. Don Gantz Boston Univ School of Medicine gantz-at-med-biophd.bu.edu 617-638-4017
We are in need of a low power, 4X, objective for the Leitz Ergolux optical microscope. I believe this is an infinity corrected optical system. Might one be available?
Joe Geller Geller MicroAnalytical Laboratory 426e Boston St. Topsfield, Ma 01983-1212 508 887-7000 fax:887-6671
The set of softwares SIMPLY-S, including for the multislice-based programs SHRLI, and dedicated to various TEM/HRTEM calculations/simulations on PCs (in the field of Materials Sciences) has recently been updated. SIMPLY-S is available from ftp.univ-lyon1.fr (PUB/DOS/HRTEM : README.TXT and SIMPLY1-2-3.ZIP files ; SIMPLY3.ZIP contains the updated version, while SIMPLY1-2.ZIP files do correspond to the previous version, to be abandoned soon).
Any microscopist interested can download the freeware version. remind also that SIMPLY-S is permanently updated (check the date in the readme.txt file).
Comments and remarks are welcome !
______________________________________________
Thierry EPICIER GEMPPM-502, INSA de Lyon, 69621 VILLEURBANNE, France tel : (33) 72 43 84 94 (83 85) FAX : (33) 72 43 85 28 Email : EPICIER-at-CISMSUN.UNIV-LYON1.FR
I seem to have missed the summary of the ISO Guide 25 Experience thread. Was it ever out there? If somebody could forward it to me I would appreciate the effort.
Thanks in advance,
Mike
Michael P. Mallamaci The Goodyear Tire & Rubber Company Analytical Sciences D/415A 142 Goodyear Blvd. Akron, OH 44305 USA -------------------------------- mallamaci-at-goodyear.com (330) 796-7436
Some one wants to look at E-Coli in the EM. I have never worked with bacteria before. Where could I find some good references on how to fix and process them. Is there a standard fix or pH or anything I should be aware of before I dive in.
} We have not seen a utility to mount Mac formatted disks on DOS systems. } } Iomapple } Iomega Technical Support
(snip)
I hold in my hand Conversions Plus from DataViz that converts files between Mac and PC programs, including floppy disks, removable cartridges (SCSI) and CR ROMS. I personally do not use it, but the good doctor in the office next door uses it quite regularly as he runs a Windows NT server for both Macs and PCs. The programs runs about $100 (US), but its smaller cousin MacOpener allows you to use Mac floppies, removable media, and CD ROMS, but not to convert. This runs about $50 (US).
The above programs apparently have done all they claim to do without a hitch, whereas he had had problems with Mac-In-Dos, a program that claims similiar capabilities.
Dennis Shubitowski University of Michigan School of Dentistry
LAMS has an ongoing need for interesting speakers for our monthly meetings.= Topics could relate to the technique, history, applications, subjects or = art involving microscopes. We are open to anything that is interesting,= even if remotely related. This could be a good way to practice a new= speech or presentation before a friendly and interested group. LAMS is a non-profit organization founded in 1938, dedicated to the study= and practice of the science, art, and history of microscopy and related= topics. Membership covers individuals with an active interest and= experience in optical and electron microscopy and associated science and= technology. LAMS has a membership of around 100 people, and an average= attendance of 40. The club has a monthly newsletter with a column by the= President Gil Melle, usually on different aspects in the history of the= microscope with artful illustrations of classic designs. We have= corresponding members all around the globe. The membership fee is $25.00= per year which includes the monthly bulletin. To join call Dave Hirsch at 3= 10-397-8357.
At this time, we conduct our meetings at the Edison Complex 1721 22nd Street= in Santa Monica California. The meetings are held every third Wednesday= of the month. Members gather about 7:00 p.m. and the program starting at= 8:00 p.m. The program usually lasts an hour and a half. There is a short= refreshment break after the program and the second activity ending at 11:00= .
The meeting room has a wall screen, blackboard,Carousel projector, large= projection video, and other items available. Please let us know your needs.
Please e-mail to the Program Chair, Larry Albright {albrite-at-leonardo.net} = or call if you are able to speak to our group, or if you would like to= simply attend a meeting. The meetings are open to anyone.=20
Larry Albright Program Chair (310-399-0865) albrite-at-leonardo.net=20
Hi you-all. I have come across a OLD Porter-Blum MT1 Ultramicrotome, but are missing collets, knife holders, & specimen trimming block. Anybody out there have any hearts to donate spare parts to make this thing sing? A copy of the operating instructions book would be helpful, too. Thanks a lot,
On Friday, April 12, 1996 the annual Spring Workshop of the Oklahoma Microscopy Society (OMS) will be held in Room 246 in the Noble Research Center at Oklahoma State University (OSU), Stillwater, Oklahoma.
The main focus of the workshop is "Digital Imaging and Image Processing" and is being presented by Dr. David Bright, 1996 MSA Speaker and a Research Chemist from the National Institute of Standards and Technology in Gaithersburg, MD. The workshop will include some of the following topics:
Image Processing for Microscopists: Practice and Pitfalls Image Processing for the Macintosh: (free software available) What are digital images and why would one want to process them? Introduction to NIH Image and MacLispix Introduction to Image Processing -aspect ratio- "correction" for tilt or non square pixels -background subtraction and normalization -direction of gradient -euclidian distance map -mathematical morphology -linear hough transform
Other items of business:
(1) Student Best Electron Micrograph Contest: Don't forget to bring those prize-winning pictures with you to the workshop. Electron micrographs will be judged by attendees at the workshop. The winner will receive a $50.00 award and the micrograph will appear on the cover of the Fall 1996 OMS Newsletter. Second place winner will receive an award of $20.00.
(2) The Fall 1996 Technical Meeting in conjunction with the Oklahoma Academy of Science will be discussed. This year it will be held at the Oklahoma State University-OKC on Friday, November 8, 1996. Abstracts are due Monday, September 16, 1996.
(3) Final call for the submission of micrographs suitable for the new OMS t-shirt. Bring your entries to the spring workshop or mail them to Ginger Baker, Sec. before that date. This contest is not limited to students. The winner will receive a first edition of this T-shirt absolutely free. Remember that the T-shirts should be considered part of OMS' outreach efforts: they will be seen mostly by the non-microscopist public. In the now-famous words of our OMS president, "No hexamethylchickenwire lattice structures, please!".
(4) Discussion on the OMS web page expertly compiled and edited by Dr. Scott Russell, University of Oklahoma.
Address: http://www.uoknor.edu/electron/oms/
From this site, you can travel to a variety of pages for up-to-date microscopy news and information!
(5) Various meetings and shortcourses coming up during the year. Also, updates on the gradeschool microscopy kits and their use in the elementary classrooms.
Preregistration for the OMS Spring Workshop is $5.00 for members, $10.00 for student non-members, and $15.00 for non-members. After April 10 add a $5.00 late fee. On site registration begins at 8:30 a.m. Lunch is included in the fee.
Lunch: smoked turkey and brisket with barbecue sauce, cole slaw, baked beans, relish, wheat/white bread, and peach cobbler.
Parking: Lot 96: Each person driving to the workshop must have a visitor parking permit to hang in the vehicle with the designated lot number.
Message-Id: {1.5.4b11.32.19960312000937.006680ac-at-itsa.ucsf.edu} X-Sender: mishot-at-itsa.ucsf.edu X-Mailer: Windows Eudora Light Version 1.5.4b11 (32) Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii"
I have used an Agfa system, chemistry and paper for 12 years. The processor was not sufficiently robust nor reliable for us. In any case it is no longer available. The Agfa Variable Contrast Premium paper is my favorite emulsion for scientific work. I recommend that you consider the Durst Printo system. It is modular so you can build it to suit your own needs. It is my choice of currently available systems.
I would appreciate any information on current user fees at university EM facilities. My institution has requested a comparison of our fees with those of other facilities. Thanks for your help.
Arthur R. Hand, D.D.S. Director, Central EM Facility Univ. of Connecticut Health Center MC 1610 Farmington, CT 06030 Tel: 860-679-4085 Fax: 860-679-4078 e-mail: hand-at-nos1.uchc.edu
Hi, I found some useful information on ZIP DRIVES FOR MIXED ENVIRONMENT.
The recent discussion on ZIP installation problems were not very encouraging but most problems may have had their cause in inadequate drivers and software.
Iomega's ZIP drives (not involved in any way with the company) are very useful for image transfer between Mac and PC, especially 12-16 bit images which do not fit on a floppy, session files, scanned images, image processing derivatives from image files etc., as well as image exchange if FTP is not available.
The following technical details come from AOL/Iomega Forum/Message Board/Topic: ZIP Technical for PC/Mac. Also, all newest Iomega drivers for Mac, DOS, Win can be copied from this site. I hope it helps. Best regards Klaus.
} Is there any way for my Mac/Zip disks to be accessed on a PC /Zip drive or } visa versa?
} } } Bernoulli, Zip, and Jaz DOS formatted disks can be mounted on a Macintosh. } The disk needs to be Preformatted or formatted on a PC using a current } version of either our Win95 or DOS/Win 3.1 utilities (Iomega Tools for } Win95, Iomega SCSI or Zip Tools). The Macintosh needs to be running our } driver version 3.5.3 for Bernoulli drives, version 4.2 or 4.3 for the Zip } drive, or version 4.3 for the Jaz drive. } } The Macintosh needs to have a DOS mounting utility. The commonly found } utilities are: } } 1. Apples' PC Exchange version 2.0.2 or greater. It is part of the Apple } System 7.5 upgrade. This version of PC Exchange can be used with any } version of System 7 and is available as a standalone product. } 2. Insignia Solutions Access PC version 3.0 or higher. } 3. Dayna Communications DOS Mounter Plus version 4.0 or higher. } } Each of these software packages will allow the Macintosh to mount DOS } formatted disks. Consult with your local software vendor for information } on which package is best suited for your needs. } } The Macintosh hierarchal file system (HFS) is not compatible with the FAT } (DOS style) file system. Because of this we recommend the following } precautions: } } 1. Create the directory structure on a PC and only copy files to the } disk. Do not create or delete folders on the disk with the Macintosh. } } 2. Have backup copies of any file you are transfering. These files should } be in their orginal format. } } 3. Disks that transfer files regularly should be formatted in a PC at } regular intervals. Once every six months should be sufficient. The more } often the disk is written to on the Macintosh, the more often this should } be. } } Important Support Note: } Each of the software packages mentioned should mount Iomega disks, just } like they would mount a DOS formatted floppy disk. If any usability, } installation, or compatibility problems are encountered contact the } software vendor for technical assistance. Iomega does not provide } technical support for these non-Iomega products. } } Problems we have seen: } } 1. You insert a DOS formatted disk into the drive and the Mac wants to } initialize it. } } a. You can get this message if another driver is controlling the drive. } Insert a Mac formatted disk into the drive and then Get Info about the } disk itself. The line that says Where will tell you what driver is in } control of the drive. At the end of the line you will see (3.5.3) for } Bernoulli drives, (4.2) or (4.3) for Zip drives, and (4.3) for Jaz } drives. Anything else would be conflict. } } b. The disk was formatted with Windows NT or a non-standard DOS format. } } 2. You try copying a singe large file to the disk and get the message } "There isn't enough room on the disk." } } a. The DOS mounting utility has enough overhead in converting the file } to limit the size of the file you can copy to the disk. It is roughly } two-thirds the capacity of the disk. } } 3. After copying over about 500 files, the files seem to become corrupt. } } a. PC Exchange has a limit of the number of files it can copy just like } DOS has a limit of the number of files that can be in the root directory. } } We have not seen a utility to mount Mac formatted disks on DOS systems. } } Iomapple } Iomega Technical Support
} } } } } } } } } } } } } } WHAT ZIP DRIVE MODELS ARE AVAILABLE? } } A Zip SCSI model and a Zip Parallel Port model. The SCSI model can be } used with Macintosh or a PC that is using a ZIP compatible SCSI adapter. } The SCSI model has two 25 pin SCSI ports (for chaining), a SCSI ID } switch, and a SCSI termination switch. The Parallel Port model can only } be used with a PC and connects to the computers Parallel Port. } } WHICH SCSI ADAPTERS CAN BE USED WITH THE SCSI ZIP MODEL? } } Iomega provides a optional low cost SCSI adapter called the Zip Zoom } Accelerator. Other ASPI compatible SCSI adapters can be used in } conjunction with Iomega SCSI driver software. } } } } } } } } } } } } } } Formatting a DOS ZIPs on the Mac (w/ PC exchange installed)? } } Yes there is a way of doing this. Go into the Tools program and click on } Erase Disk. On the next window, select the long, ten minute format. Start } the format and then stop it after five seconds and quit Tools. This will } pop the disk out. Reinsert the disk and PC Exchange should take over and } ask how you would like it formatted. } } We have seen problems doing it this way. Sometimes the newly formatted DOS } disk isn't readable. We suggest that the formatting be done on a DOS } system. } } Iomapple } Iomega Technical Support
I hope these clarifications will help using the ZIP diks for image transfers. For future reference, I have included this info in my web page. Thanks Klaus
I'm trying to contact the manufacturer of a power supply in our Oxford color monitor, made in the U.K. Could someone in the U.K. help me by providing an address & phone number for Cotron Electronics Ltd.?
Thanks. Owen P. Mills Michigan Technological University Metallurgical & Materials Engineering Rm 512 MME Building Houghton, MI 49931 906-487-2002 906-487-2934 FAX opmills-at-mtu.edu
The complete alignment of the CM30 takes about 1 hour, so if you happen to have a computer connected to the microscope (allowing you to save and load alignments data) it might be worth trying the alignment.
By the way I have another question about light variation. On our CM30, the position of the light varies in a notable way depending on the C2 condensor excitation. If the C2 current is set at maximum, then back to cross-over we do not get the light at the same place than if we set C2 current at minimum and then back to cross over. (at X 20000 the light gets nearly out of the screen. I wrote to Philips, they told me that the microscope was "within specification". I would have liked a more elaborated answer, or even new inputs from CM microscopes users.
Yves MANIETTE Universitat de Barcelona Serveis Cientifico Tecnics Unitat ESCA TEM Carrer Luis Sole i Sabaris E-08028 BARCELONA
Tel +34 3 402 16 95 Fax +34 3 402 13 98
On Fri, 8 Mar 1996, Wharton Sinkler wrote:
} } Hello all: } } I am using a Philips CM30ST for high resolution imaging. I have had a } problem performing the critical objective lens direct alignment "current } rotation center". Ideally, one should obtain a stationary point on the } specimen, rotating about its center at high magnification with a } low-frequency wobble applied to the objective lens current. I can get } the stationary point on the specimen reasonable satisfactorily. } The probjem is that the beam moves fairly significantly when the objective } lens wobbler is on. } } I have performed the coma free direct alignment, which should ensure that } the beam is travelling along the optic axis of the objective lens. This } works well. However, it seems to me that if wobbling the objective lens } current can shift the beam, it can't be travelling the optic axis. } } Is there any relatively quick way to correct the problem of beam shift } with objective lens current change? I can imagine that a complete } realignment of the microscope might get rid of the problem. However, } this is both very time-consuming and also not very instructive as to the } cause of the problem. } } Wharton Sinkler } } ================================================== } } Dr. Wharton Sinkler } GKSS Forschungszentrum Geesthacht } Abteilung WA } D-21502 Geesthacht } Germany } } tel: (49)(4152)872542 } fax: (49)(4152)872534 }
Another idea: if you know someone with access to a decent machine shop, and is skilled, you can have the scope column sawn in half longitudinally to expose the parts _in situ_. We have a couple of TEMs treated in this way on display, and they are useful teaching tools. (This was done many years ago by an engineer who used to work here.)
&&& Illigitimi non carborundum &&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&& Philip Oshel Center for Electron Microscopy University of Illinois (217)244-3145 oshel-at-ux1.cso.uiuc.edu
Message-ID: {199603091507.KAA28839-at-IndyNet.indy.net} To: Microscopy list {microscopy-at-Sparc5.Microscopy.Com} , SPM List {spm-at-di.com}
I am trying to find any program that will allow to calculate the isolated islands area and their size distribution using digi- tal TEM images of metal or metal oxide thin films.
Thanks for any help that may be offered. Vladimir Kukin, Electron Microscopy Lab, Moscow Institute of Electronic Technology (Technical University) lemi-at-mx.iki.rssi.ru
In order to get information aboput the excellent SEM and XRMA Short Courses being offered by Lehigh University contact Ms. ShAron Coe by e-mail at slc6-at-lehigh.EDU Sharon is a prompt e-mail replyer. See you in June
Patrick Echlin University of Cambridge UKOn Wed, 6 Mar 1996, MARK DARUS (216) 266-2895 wrote:
} Could someone send me information, or an E-Mail contact, for } Lehigh (spelling may be wrong), in PA, so I can get information } on courses offered. } } Thanks } } Darus-at-cle.dnet.ge.com }
POSITION AVAILABLE: Associate Director, Molecular Cytology Core Facility (confocal scanning laser microscopy, low light video microscopy, image processing/analysis, microtomy, immunocytochemistry and in situ hybridization). Individual with experience in some or all of these areas is sought to train users, maintain instruments, develop protocols for a multiuser facility. PhD desirable but not required for individuals with extensive experience. Women and minority candidates are especially encouraged to apply. Address applications (CV and 3 letters of reference) or inquires to: Thomas E. Phillips, Ph.D. Division of Biological Sciences 3 Tucker Hall, University of Missouri Columbia, MO 65211 573-882-4712 tphillips-at-biosci.mbp.missouri.edu.
Many thanks to all who responded about my Polaroid film dilemna. The emulsion ended up washing right off in an overnight soak. Hopefully this does not happen again. If it does, however, I am armed with an arsenal of tips!
Thanks again,
Dennis Shubitowski University of Michigan School of Dentistry
Here is a summary of the responses:
I have never been successful in separating T55 negatives that have dried together.
John chandler-at-lamar.ColoState.EDU
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I did SEM for 7 years at Wayne State and ran into that problem at least several times. Let me tell you straight: it can't be done and isn't worth the effort. You'll always get some junk to remain on the negative ruining it forever. Oh, I hope the negatives aren't too important!
Walt Bobrowski Parke-Davis Research
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Have you tried a little detergent in the water? The liquid type is the best to use.
I don't know if this is a sure thing, I always used a water soak, but my success may be due to the local water.
Hope this helps.
Ellie Solit The Microscope Book
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I have used a AGFA AGEPON-WATER SOAK on TEM nagatives before That worked
Stephan H Coetee Electron Microscope Unit Private Bag 3 Wits 2050 Stephan-at-Gecko.biol.WITS.ac.za
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Yes, I have successfully unstuck pairs of Polaroid negs. Actually, the water soak will work, but you have to soak them for a long time, at least overnight, say 12-16 hours. Then you have to slowwwwwwwly pull them apart to avoid pulling the emulsion off. A "pucker" mark may remain where they were joined, but usually its translucent so that a positive print will seldom reveal the pucker mark, especially on "busy" image detail, and as long as original image density was not disturbed.
When this happens to my negs, its usually in a spot no larger than the size of a quarter (25 cent piece). If its most of the area of the negs, that is serious trouble.
When I dry Polaroid negs in racks, I skip slots so that there is lots of space between adjacent negs to prevent sticking from occuring as the negs "wiggle" as they air dry.
Gib Ahlstrand, MMS Newsletter Editor Electron Optical Facility, University of Minnesota, Dept. Plant Pathology 495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249 612-625-9728 FAX, giba-at-puccini.crl.umn.edu
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I have sometimes (!) been successful by giving the negatives a prolonged soak (days) in water. the problem, of course, is that the emulsion can be washed off - you have to hope that the negatives unstick before you lose the emulsion. Good luck!
Tony Garratt-Reed
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I remember a water soak working for me, but not without waiting over a weekend. Even then it left a patch ...
{\/} /\ {\/} /\ {\/} /\ {\/} cognito, ergo zZOooOM {\/} /\ {\/} /\ {\/} /\ {\/} Michael Shaffer, R.A. - University of Oregon Electron Probe Facility mshaf-at-oregon.uoregon.edu -or- mshaf-at-darkwing.uoregon.edu http://darkwing.uoregon.edu/~mshaf/epmahome/
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I have had limited success with an overnight Photoflo soak followed by ultrasonication. Films stuck emulsion to film back seem to separate easier than those stuck emulsion to emulsion.
richard.mount-at-mailhub.sickkids.on.ca
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I agree with Richard, above, that film back stuck to emulsion is easier to seperate, and this is the case I usually run into as I dry my Polaroid negs in racks with the emulsion of one facing the back of the next neg.
A point that I left out in my previous note describing successful use of overnight water soak to seperate stuck negs is how I set this up. As with many techniques, success lies in the details of the method. I load a pair of stuck negs into my usual neg drying rack with edges of one neg in one set of slots, edges of other neg in adjacent set of slots. The effect is to separate the negs where they are not stuck to enable water to get directly to the stuck area. Success depends on getting good water soak and softening of the film emulsion. If you were to simple lay the two stuck negs flat in a pan of water, might not get as good a soak as when the films are seperated a bit to allow water access.
I hope this helps.
Gib Ahlstrand, MMS Newsletter Editor Electron Optical Facility, University of Minnesota, Dept. Plant Pathology 495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249 612-625-9728 FAX, giba-at-puccini.crl.umn.edu
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YES, virginia, polaroid negatives can usually be unstuck, just soak in very hot water about 10 -20 minutes, then GENTLY pull them apart, GRADUALLY turning and pulling a little untilthe whole gelatine that is stuck gets soaked through and losened. Some times if more completely dried out, the emulsion of one will be stuck to the second, usually the second can be saved completely, but some of the first will be lost (natually the center, best part of the image) good luck
Alan S. Pooley ,PhD Bivalve shell SEM & shape analysis SEM/Morphometrics lab Marine & Coastal Sciences Rutgers University 908 932 8959 ext 225 Pooley-at-ahab.rutgers.edu
} Dear Mary, I read your notice about Stan klosevych's papers in the MSC } bulletin, } and tried to locate the NSC home page on the web address you gave. My computer } running under Netscape did not recognise this. i would still like to know more } of Stan's articles, as i teach microscopy. Can you help. TIA Jeremy Sanderson. Dear Jeremy, As several of my friends informed me, the Web site I gave for the MSC had a small typo that made it unusable. In my defense, I copied it correctly from the current MSC Bulletin. They had it wrong. The correct address is: http://gause.biology.ualberta.ca/craig.hp/MSC.SMC/Mic.Soc.hp As I said, there is also a link from the MSA WWW site. Regards, Mary
Mary Mager Electron Microscopist Metals and Materials Eng, UBC 309 - 6350 Stores Rd. Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648, fax: 604-822-3619 email: mager-at-unixg.ubc.ca
For your Universal M microscope equipped with the "Vertical Illuminator IIC", you will need a 22mm diameter glass plate (semi- transparent mirror). You can install this into your "H-PL" housing.
It is still available as a "spare part" from Zeiss under catalog number 466260-0010 at U.S. $72.00. For ordering spare parts, please call U.S.: 800/233-2343 Canada: 800/387-8037
The other option you always have is to call one of the companies which manufacture all kinds of filters such as
You wrote " I have a Zeiss Universal 'M' microscope which is missing a few } parts, which are now VERY (?) expensive (from Zeiss). } } I require a REFLECTOR for a 'Vertical Illuminator IIC'; either a } H-PL, or H-PR will do. Only the reflector is required as I have the } housing. Does anyone have one of these ? Or does anyone know if it is } possible to 'make' one using a 'double sided semi-transparent mirror' ? } ============================================================================== } Steve Schwarz sschwarz-at-morgan.ucs.mun.ca } Dept. of Earth Sciences } Memorial University of Newfoundland } Newfoundland } CANADA } A1B 3X5 } 1-709-737-8142 } -737-2589 FAX
We decomissioned our Stereoscan Mk II after 25 years of service, as an instrument with open (graduate) student access. Managing the electronic tubes just became impossible. The Engineering School mantains a Museum, were we gave it honourable burial. But first we took out pumps, oscilloscope, camera, some gauges, etc. which continue to serve usefully. The empty shell still looks nice, and who would know the difference. It is now among scale models of locomotives, our first computer, etc. This instrument was installed in 1969. We know it was the first SEM in Brazil, probably also in South America, and we like to think it might have been the first in the Southern Hemisphere. Anyone like to comment on this? Prof.Walter A.Mannheimer Metallurgy and Materials Engineering Federal University of Rio de Janeiro POBox 68505 21945 Rio de Janeiro Brazil Voice 5521 280-7443 (Dept.office) 5521 590-0579 (direct) Fax 5521 290-6626 Email: wamann-at-metalmat.ufrj.br
I will receive Candida albicans samples in my lab for service. I am looking for a good preservation of cell wall, cell membranes, nuclei, mitos, ribosomes, etc. I must keep the cell wall intact for this study. ALso, I want to avoid acrolein.
Does anyone have a solution ?? ANy advise?
Thanks in advance
Theresa
Dr. Theresa A. Fassel Sr. Research Associate fassel-at-post.its.mcw.edu Department of Microbiology (414)-456-8410 Medical College of Wisconsin Fax (414)-266-8522 8701 Watertown Plank Road Milwaukee, WI 53226-0509
I have done Yeast, lichen, soybean and tobacco seedlings with microwave fixation and processing, and have been quite pleased with the vast improvement in results.
I have used both a standard microwave and a nice one from Ted Pella specifically for EM.
There are many ways to do this, but the following is what we follow in our lab:
Microwave rules: 1. Always use at least a 250 ml water load to collect the heat and keep it off your sample. I change this every 16 seconds of use ( 2 fixations). **** Always set the water load in the same place each and every time.
2. Calibrate the Microwave. Set the water load/ beaker in it's designated spot. Use liquid crystal sheets and microwave about 15 seconds. -- Observe where the hot spots show up, and avoid them. Pick a place preferable inbetween hot spots.
3. Always do a pilot study first.
4. Things to avoid like the plague:
--- Always chill your sample in wetted down crushed ice for at least 30-60 seconds before microwaving. This will allow MW, but keep heat off your sample. **** As soon as you are finished microwaving , put back into the ice for a moment.
---- Always wipe off the outside of your vial, for water draws heat ( hence the water load situations) Also never allow water to be anywhere but in the waterload beaker.
--- Using the same principle, only use enough fixative/ solution to just cover the sample. I usually use about 1-2 mm high. Greater volumes will EXPONENTIALY (sp) increase the heat load to your sample. The difference between 6 drops and 1 ml can be 40C!!!!!
Processing times: ==============
You have to experiment with this.
I'd start out with possibly the following for things with thick walls and for plants:
1. Ice sample, wipe, microwave for 7 seconds, ice.
2. Repeat the above for a total of 3 times.
3. Rinse in buffer.
4. Put into OsO4---2%aq.
Ice, MW 7 seconds, let set 10 min................change solution and repeat once more. And save the last OsO4 on the sample.
****** it is amazing how MW speeds up processing times, for the first time ever after starting microwave, I have to really watch OVER staining enbloc. So...... repeat a 3rd time if you don't like the staining of the first, but try doing only 2 OsO4's at first.
5. To the last OsO4 solution on sample, Add equal volume of 3% Potassium Ferricynide. Set for 20 minutes only.
6. Rinse with water.
7. Add filtered saturated aq Uranyl Acetate, no older than 10 days. ( Might be our water problem)
Ice, MW 7 seconds Ice, and let set for 15 minutes at room temperature.
REPEAT
8. Dehydrate as norm. 8-10 min. each step.
9. Infiltration: -- these will get warm, it's OK, and even helps greatly.
1:1 MW 10 seconds, no ice, incubate (rotating)15 minutes and REPEAT
1:4 MW 10 seconds, no ice, incubate 1 hour and repeat.
Pure epoxy: MW 10 seconds, no ice Incubate 1 hour intervals and overnight. Repeat MW in am, make at least 2 changes of epoxy.
** Also helps to spend overnight in vacuum in a desicator hooked up to house vacuum.
The difference I've seen in structure, staining and non-pitting out of the specimens vs the more traditional methods I've used is incredible. We like microwave so much we now use it for all of our samples.
I'm going to be leaving for a week, but if you'd like to know more, I can get you references etc when I'm at work when I get back. Our methods are based on Logan's, Ted Pella's and my 2 years of working through techniques.
Good Luck,
Lou Ann
} Hi } } I will receive Candida albicans samples in my lab for service. I am } looking for a good preservation of cell wall, cell membranes, nuclei, mitos, } ribosomes, etc. I must keep the cell wall intact for this study. ALso, } I want to avoid acrolein. } } Does anyone have a solution ?? ANy advise? } } } Thanks in advance } } Theresa } } Dr. Theresa A. Fassel } Sr. Research Associate fassel-at-post.its.mcw.edu } Department of Microbiology (414)-456-8410 } Medical College of Wisconsin Fax (414)-266-8522 } 8701 Watertown Plank Road } Milwaukee, WI 53226-0509
*************************** Lou Ann Miller Microscopic Imaging Lab College of Vet. Medicine University of Illinois 2001 S Lincoln Ave Urbana,Illinois 61801 217-244-1566 lmiller-at-ux1.cso.uiuc.edu
Microscopy Home Page: http://www.cvm.uiuc.edu/MicImagLab/MicImagLab.html
Personal Home Page: http://www.cvm.uiuc.edu/HomePages/LouAnnMiller/LAM.html
Subject: Time: 5:05 PM OFFICE MEMO RE:Origin of C Particles Date: 3/11/96
It may well be that by carrying out a proper analysis of your materials using the various technidques of optical microscopy (polarized light, refractive index meas, etc.), you can get info about the source of the carbon particles. The people at McCrone Associates Inc (850 Pasquinelli Dr, Westmont, IL 60559 (Ph: 708-887-7100, Fx: 708-887-7417) are world reknown specialists in identifying particulate materials and determining their sources. I suggest you get in contact with them. W. C. Bigelow (bigelow-at-umich.edu)
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Scanning Microscopy International P.O. Box 66507, Chicago (A.M.F. O'Hare), IL 60666-0507, U.S.A. Telephone: (708) 529-6677 / FAX: (708) 980-6698 E.mail: 73211.647-at-compuserve.com
Scanning Microscopy 1996 meeting May 11-16, 1996, Bethesda, Maryland (suburb of Washington, DC)
Symposium on: Scanning Probe Microscopies and Related Techniques for the Biological and Materials Sciences
Note: Nearly 35 SPM related papers will be presented during the program on "Pattern Formation and Nanoscaled Structures in Thin Film Formation" at the same time at same venue. THOSE PAPERS ARE SEPARATELY LISTED BELOW.
Program organizers: Dr. David P. Allison, Oak Ridge Natl. Lab., TN (E-mail: allisondp-at-ornl.gov); Prof. Chunli Bai, Chinese Acad. Sci., Beijing (E.mail: clbai-at-infoc3.icas.ac.cn); Prof. Masamichi Fujihira, Univ. Yokohama, Japan (E.mail: mfujihira-at-bio.titech.ac.jp); Dr. Heinrich J.K. Hoerber, European Molec. Biol. Lab., Heidelberg, Germany (E.mail: hoerber-at-embl-heidelberg.de); and Prof. Douglas J. Thomson, Univ. Manitoba, Winnipeg, Canada (E.mail: thomson-at-ee.umanitoba.ca).
VERY FEW papers can still be offered: please contact one of the organizers or Dr. Om Johari at Scanning Microscopy International.
The registration fee for the entire 6-day conference (without subscription to any of the SMI journals' 1996 volumes) is either $100 (if payment reaches SMI before March 31, 1996) or $120 (from April 1, 1996); the fees with 1996 subscription to one, two or all three SMI journals (Scanning Microscopy, Cells and Materials, and Food Structure) are respectively: $180, $220, or $270 (for US delivery addresses), or $210, $270, or $340 (for outside US addresses). The room rates (exclusive of applicable taxes, currently 15%) at the Hyatt Regency Bethesda (One Bethesda Metro, Bethesda, MD 20814, USA; phone: 301 657 1234; FAX 301 657 6453) are: Single (1 person - 1 bed): $100; Double (2 persons - 1 bed) or Twin (2 persons - 2 beds): $110. Please make room reservations directly with the hotel.
List of presentations (as of March 10, 1996) in alphabetical order (the name of the presenter is capitalized when there are more than one authors)
M.J. ALLEN, Digital Instruments, Santa Barbara, CA; E.M. Bradbury, R. Balhorn: The Chromatin Structure of Well-Spread Demembranated Human Sperm Nuclei Revealed by AFM
D.P. ALLISON, P.S. Kerper, M.J. Doktycz, T. Thundat, R.J. Warmack, Oak Ridge National Lab., TN: High Resolution Physical Mapping of EcoRI Restriction Sites on Intact Cosmids by AFM Imaging
P.C. Zhang, C. BAI, P.K.H. Ho, Q. Li, Y. Dai, Y.S. Wu, B.F. Sheng, Chinese Acad. Sci., Beijing, China: AFM Study of Interactions Between Tumor Necrosis Factor and Its Monoclonal Antibodies
A.A. BERGMAN, A.P. Quist, C.T. Reimann, S.O. Oscarsson, B.U.R. Sundqvist, Uppsala Univ., Sweden: Antibody-Antigen Docking Observed with Tapping-Mode Scanning Force Microscopy in Air
A.A. BUKHARAEV, N.V. Berdunov, D.V. Ovchinnikov, Kazan Physical- Technical Inst., Russia: Three-Dimensional Probe and Surface Reconstruction for Atomic Force Microscopy Using Deconvolution Algorithm
R.J. Pylkki, G.J. COLLINS, Topomatrix, Santa Clara, CA:
Scanning Thermal Microscopy with a Positive Probe
E.D. DAHLBERG, R. Proksch, S. Foss, G. Skidmore, C. Merton, Univ. Minn, Minneapolis: Review: Magnetic Force Microscopy and Magnetotactic Bacteria
P. DEWOLF, T. Trenkler, T. Clarysse, W. Vandervorst, IMEC, J. Snauwaert, L. Hellemans, Leuven, Belgium: Electrical Characterization of Submicrometer Silicon Devices by Cross-Sectional Contact Mode AFM (to be presented in the Semiconductor program)
S.J. EPPELL, R.E. Marchant, Case Western Reserve Univ., Cleveland, OH: Atomic Force Microscopy of Bone: More than an Image (to be presented during the Bone Biology program)
R. ESCHRICH, Max-Planck-Inst. Biochem., Martinsried, Germany; G.L. Kumar, T.A. Keil, R. Guckenberger: AFM on the Olfactory Dendrites of Giant Silkmoth Antheraea
G.L. Kumar, Max-Planck-Inst. Verhaltenphysiol., Seewiesen, Germany; R. ESCHRICH, R. Guckenberger, T.A. Keil: In Search for Putative Pheromone Receptors on the Membrane of Olfactory Dendrites in Silkmoths (A. polyphemus and A. pernyi) Using the AFM and SEM
M. Fujihira, Tokyo Inst. Tech., Japan: Review: AFM of Solid Surfaces in Aqueous Solutions
L.A. GHEBER, J. Hwang, M. Edidin, Johns Hopkins Univ., Baltimore, MD: Imaging of Rough Biological Samples in Liquid with Fluorescence Near Field Scanning Optical Microscopy
D.T. GODDARD, A. Steele, I.B. Beech, British Nuclear Fuels, Preston, UK: AFM of Bacterial Biofilms with Application to Microbially Influenced Corrosion
H. Hansma, Univ. Calif., Santa Barbara: Review: Atomic Force Microscopy of Biomaterials
M. HARA, W. Knoll, RIKEN, Saitama, Japan: Review: STM and AFM Studies of Self-Assembled Monolayer Growth
D.O. HENDERSON, Y.S. Tung, R. Mu, Fisk Univ., Nashville, TN; W.A. Curby, M. Mercado, Fed. Aviation Rech. Ctr., Atlantic City, NJ: Optical and Atomic Force Microscopy of Pentaerythritol Tetranitrate Nanoclusters on Si(100)
C.L. Mosher, E. HENDERSON, Iowa State Univ., Ames: Review: Chemical and Biomolecular Force Detection
R. Eckert, S. Jeney, W. Oeffner, J.K.H. HOERBER, European Molecular Biology Lab., Heidelberg, Germany: Measuring Surface Forces with the AFM
D.H. HUANG, Y. Yamamoto, Yamamoto Quantum Fluctuation Project, Tokyo, Japan: Hydrogen Atom Extraction and Redeposition on Hydrogen-Terminated Silicon Surface with Ultra High Vacuum STM at Room Temperature
A. IKAI, Tokyo Inst. Tech., Japan, K. Mitsui, M. Hara: Review: Measurements of Mechanical Parameters of Proteins and Chromosomes
A. IKAI, H. Takeuchi, S. Kawauchi, Tokyo Inst. Technol., Japan STM Study of Stearoyl Amide and Anilide
M.D. JOHNSON, Univ. Oklahoma, Norman, and H.W.M. Salemink: Review: Cross-Sectional STM on Semiconductor Heterostructures (to be presented during the Semiconductors program)
I. KARL, J. Bereiter-Hahn, Univ. Frankfurt, Germany: Regulation of All Surface Motility Revealed by Scanning Acoustic Microscopy
L. KUUTTI, J. Pere, J. Peltonen, O. Teleman, VTT Biotech. Food Res., Espoo, Finland: Interpretation of High Resolution AFM Images of Crystalline Cellulose Using Molecular Modelling
R.M. LeBlanc, Univ. Miami, Coral Gables, FL: Review: Scanning Probe Microscopies of Langmuir-Blodgett Films
G. LEE, A.D. MacKerell, L.A. Chrisey, R.J. Colton, US Naval Res. Lab., Washington, DC: Measuring Inter- and Intramolecular Forces in Biomolecules
A.L. LITVIN et al., US Army Natick R&D Center, Natick, MA AFM and Optical Studies of Organic Thin Films with Hydrogen-Bonded Networks
L.S. Shlyakhtenko, S. Adhya, T. Aki, Y. LYUBCHENKO, Arizona State Univ., Tempe: AFM Studies of Regulatory Nucleoprotein Complexes
F.K. MANTE, Univ. Pennsylvania Dental School, Philadelphia, G. Baran: Nanoindentation Studies of Single Crystal Titanium
J.F. Marchiando, NIST, Gaithersburg, MD:: Methods for Interpreting Measurements from a Scanning Capacitance Microscope (to be presented during the Semiconductors program)
M.T. MCDERMOTT, Univ. Alberta, Edmonton, Canada, J.-B.D. Green, M.D. Porter: Review: Chemical Mapping with Force Microscopy
M. Rivera, M. MILES, R.L. Williamson, Univ. Bristol, UK: Phase Transitions in Liquid Crystals Observed by SPM
W. MIZUTANI, M. Motomatsu, H. Ogiso, H. Tokumoto, Nat. Inst. Adv. Interdiscpl. Res., Tsukuba, Japan: STM with Local Non-Linearity Detection of Organic Thin Films and Ion Irradiated Graphite
D.J. MUELLER, F. Schabert, A. Engel, Univ. Basel, Switzerland: Review: Structural Changes of Native Membrane Proteins Monitored at Subnanometer Resolution with the AFM
F. MUELLER, A.-D. Mueller, Tech. Univ., Chemnitz, Germany: Scanning Capitance Microscopy of Composite Materials
H. MURAMATSU, Seiko Instruments, Chiba, Japan; T. Ataka, N. Chiba, K. Nakajima, M. Fujihira Review: Fluorescence Imaging and Spectroscopy of Biomaterials in Air and Liquid by SNOM/AFM
R. Perez, Univ. Cambridge, UK: First Principles Simulations of Atomic Resolution in Non-Contact AFM (to be presented during the Fundamental Physics Program)
R. RAITERI, Univ. Genoa, Italy, H.-J. Butt, Max-Planck-Inst. Biophy., Frankfurt, Germany: Changes in Surface Stress Measured with an AFM
B. SAMORI, A. Gordano, I. Muzzalupo, Univ. Bologna, Italy: DNA Deposition on Mica for Scanning Force Microscopy Imaging
W. Haiss, J.K. SASS, Fritz-Haber-Inst., Berlin, Germany: STM Surface Stress Measurements in Electrochemistry (tentative)
A.J. Simon, Merck Res. Lab., West Point, PA: Review: Molecularly Combed DNA: First Stretched, Then Imaged
R.P. Singh, Indian Min. Sci. Tech., New Delhi: STM and Application Possibilities
Y. Shirane, Univ. Tokushima, Japan: Surface Observation of Calcium Oxalate Monohydrate Crystals by FE- SEM and AFM (to be presented during the Stones and Crystals program)
F. El Feninat, C. Poulin, T. Ellis, E. Sacher, I. STANGEL, McGill Univ., Montreal, Canada: The Applications and Limitations of AFM to Complex Biomaterials Surfaces: Effects of Acid Demineralization on Human Dentin
M.S. UNLU, B.B. Goldberg, Boston Univ., MA: Review: Characterization of Materials and Devices by Near Field Optical Scanning Microscopy (to be presented during the Semiconductors program)
J. Vesenka, Calif. State Univ., Fresno: Review: The Diameter of Duplex and Quadruplex DNA Measured by SPM
VU THIEN BINH, F. Feschet, V. Semet, S.T. Purcell, Univ. Lyon, France: Fresnel Projection Microscopy: Theory and Experiment: Electron Microscopy with Nanometer Resolution at ~ 200 eV
C. Lebreton, Z.Z. WANG, CNRS Lab. Microstr. Microelec., Bagneux, France: Pulse Width Dependence of Nanowriting
G. Brown, M.B. WEIMER, Texas A&M Univ., College Station, TX: Adsorption and Interaction of Ammonia Molecules on GaAs(110) Studied with STM
F. Zenhausern, IBM Watson Research Ctr., Yorktown Hts., NY: New Developments in Near Field Optical Microscopy/Spectroscopy
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Please circulate / Publicize
Scanning Microscopy International Post Office Box 66507, Chicago (A.M.F. O'Hare), IL 60666-0507, U.S.A. Telephone: (708) 529-6677 / FAX: (708) 980-6698 e.mail: 73211.647-at-compuserve.com
Scanning Microscopy 1996 meeting; May 11-16, 1996, Bethesda, Maryland
Program on:
Pattern Formation and Nanoscaled Structures in Thin Film Formation
Organized by: Prof. Martin Zinke-Allmang, Univ. Western Ontario, London, Canada (E-mail: M.Zinke-at-uwo.ca; FAX 519 661 2033), Prof. Hiroshi Iwasaki, Osaka, Japan (E-mail: iwasaki-at-sanken.osaka-u.ac.jp; FAX: 81-6-8798404) and Prof. Ellen D. Williams, Univ. Maryland, College Park (E-mail: williams-at-surface.umd.edu; FAX: 301 314 9465).
Program Focus: (1) Surface and Interface Defect Formation and Structure; (2) Phase Separation on Surfaces and Epitaxial Growth Modes; (3) Surface Roughness, Step Formation, and Dynamics During Growth; (4) Characterization Techniques with a Focus on Electron Microscopy and Tunneling Methods; (5) Synthetic Sublithographic Nanostructures; and (6) Defect Engineering and Related Applications for Mismatched Materials.
A FEW Papers can still be offered, please contact one of the organizers or Dr. Om Johari at Scanning Microscopy International.
List of presentations (as of March 11, 1996) in alphabetical order
P. Campbell, Naval Res. Lab., Washington DC: Nanofabrication with Proximal Probes
G. Carlow, Univ. West. Ontario, London, Canada: Hummock Formation and Evolution During Ion Bombardment of Rotating Substrates
O. Enea, Univ. Poiters, France: Topographic Studies of TiO2 and SnO2 Ceramics by Environmental Scanning Electron Microscope, SEM and AFM
R. Feenstra, Carnegie Mellon Univ., Pittsburgh, PA: STM Studies of Semiconductor Thin Film Structures
D. Gerthsen, Univ. Karlsruhe, Germany: Atomic Scale Investigation of the Mismatch Relaxation in In0.6Ga0.4As/GaAs(100)
M. Grant, McGill Univ., Montreal, Canada: Theoretical Studies of the Dynamics of Nanostructure Formation
M. Hong, AT&T Bell Labs., Murray Hill, NJ: Novel Heterostructures Fabricated Using In-Situ Molecular Beam Epitaxy
J.W.P. HSU, Q. Xu, Univ. Virginia, Charlottesville, E.A. Fitzgerald, Y.H. Xie, P.J. Silverman: Scanning Probe Microscopy Studies of Electrically Active Defects in Lattice Mismatched Films
R. Hull, Univ. Virginia, Charlottesville: Real-Time Nanostructural Studies of Defects and Degradation Modes in Semiconductor Materials and Devices
M. Ichikawa, JRCAT, Tsukuba, Japan: Observation and Formation of Nanostructures Using a Combined Microscope with Scanning Reflection and Scanning Interference Electron Microscopes and STM
A. Iwamoto, T. Yoshinobu, H. IWASAKI, Osaka Univ., Japan: Scanning Probe Microscopic Studies of Surface Kinetic Roughening
H.-C. JEONG, J.D. Weeks, Univ. Maryland, College Park: The Dynamics of Steps on Vicinal Surfaces During Reconstruction-Driven Faceting
H. KIRK, Z. Radzimski, G. Rozgonyi, North Carolina State Univ., Raleigh: Electron Beam-Induced Current (EBIC) Characterization of Defects in SiO2 and SiO2/Silicon Interface
A. Kowal, Polish Acad. Sci., Krakow, Poland: Properties of Nickel Hydroxide Layer Formed Electrochemically on the Surface of Polycrystalline Ni in 1M KOH Studied by In Situ Atomic Force Microscopy
M. KUNITAKE, N. Batina, K. Itaya, JRDC/ERATO: Itaya Electrochemistry Project, Sendai, Japan: In Situ STM Study of Self-Organized Molecular Layers on an Iodine-Modified Au(111) Surface
S. MATLIS, H. Ron, I. Rubinstein, Weizmann Inst. Sci., Rehovot, Israel: AFM Study of Gold Oxide Stabilized with Self-Assembled Monolayer
K. Matsumoto, Electrotechnical Lab., Tsukuba, Japan: Application of STM/AFM Nano-Oxidation Processes to Room Temperature Operated Single Electron Transistors and Other Devices
J.J. McClelland, NIST, Gaithersburg, MD: Nanofabrication via Laser Focusing of Atoms
I. Nevernov, Technobiochip, Marcina, Italy: Sub-Micron Machining of Al Tracks Deposited onto a Silicon Wafer
R. Nishitani, T. Umeno, A. Kasuya, Y. Nishina, Kyushu Inst. Technol., Iizuka, Japan: Correlation Between STM-Induced Photon Mapping and the STM Topography of Nanometer-Size Metal Particles
D.D. PEROVIC, B. Bahierathan, Univ. Toronto, Canada, D.C. Houghton, H. Lafontaine, J.-M. Baribeau: Morphological Instability Phase Diagrams: Mapping Thin Film Strain Relaxation Behaviour
C. K. Shih, Univ. Texas, Austin: Scanning Probe Microscopy and Spectroscopy of Semiconductor Heterostructures
J. Stroscio, NIST, Gaithersburg, MD: Iron and Chromium Growth Studies
I. TERESHKO, V. Khodyrev, E. Lipsky, Mechanical Engg. Inst., Mogilev, Belarus: Self-Organizing Processes and Modification of Thin Films by Scanning Microscopy
R. Tromp, IBM, Yorktown Hts., NY: LEEM Related Studies and Optical Microscopy of Semiconductors
L. Whitman, Naval Res. Lab., Washington, DC: The Atomic Scale Structure of Semiconductor Surfaces: What Makes the Atoms Smart?
S.-L. YAU, Y.-G. Kim, K. Itaya, JRDC/ERATO: Itaya Electrochemistry Project, Sendai, Japan: Scanning Tunneling Microscopy of Chemisorbed Benzene on Rh(111) and Pt(111) Electrodes in Hydrofluoric Acid Solution
J. ZEGENHAGEN, Max-Planck-Inst. Festkoerperforschung, Stuttgart, Germany, P. Lyman, M.J. Bedzyk, M. Boehringer: Review: Analysis of Microscopic Structure of Stress Induced Reconstructions on Semiconductor Surfaces
M. ZINKE-ALLMANG, T.D. Lowes, Univ. Western Ontario, London, Canada: Cobalt Clustering on Si at Low Temperatures
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Other related programs at the same venue (*: Fliers available):
*Fundamental Physics in Microscopy and Microanalysis (~ 25 papers),
*Scanning Microscopy and Semiconductors: Metrology and Diagnostics (25+ papers);
Several Biological programs including: Microanalysis and Imaging (12 papers), Immunolabelling (8 papers), *Radiation Effects / Apoptosis (25+ papers), *Dentistry (20+ papers), Corrosion Casting (including a program on Lung: 10 papers), Inner Ear (6 papers), *Bone Biology (35 papers), *Stones and Crystals (35 papers);
Several Biomaterials Related Programs organized under "*Cells and Materials": (1) Skeletal Tissue / Biomaterials; (2) Foreign Body Reactions; (3) Biointerfacial Reactions at Biomaterial Surfaces; (4) Innovative Drug Delivery Systems; (5) Blood-Related Biomaterials; and (6) Dental Biomaterials. (nearly 70 papers)
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For more information, please complete and return the form below to SMI:
__ I wish to present at the Scanning Microscopy 1996 meeting (tentative title and summary on a separate sheet), please send a Letter of Intent form. IT IS VERY LATE NOW!
__ I cannot present, but am likely to attend the 1996 meeting, please keep me informed and send me: ___ 1996 Registration / Hotel form.
__ I can neither present nor attend; please add/keep my name on your mailing list. ___ Send me a mailing list form.
Please send: 1996 program fliers (*list programs here):
___ Instructions for Authors / ___ Major subject index / Table of Contents for: ___ Scanning Microscopy / ___ Cells and Materials / ___ Food Structure;
Flier on Scanning Microscopy:
___ Supplement 6, 1992 ("Signal and Image Processing in Microscopy and Microanalysis");
___ Supplement 7, 1993 ("Physics of Generation and Detection of Signals Used for Microcharacterization");
___ Supplement 8, 1994 ("Science of Biological Microanalysis")
___ Flier on the special issue: Interface Formation and Dynamics in Layered Structures (Scanning Microscopy, Vol. 8, no. 4, 1994).
___ Flier on 1996 Pfefferkorn Conference on Electron Image and Signal Processing, May 18-22, 1996 at Silver Bay, New York
We have a Hitachi 2460N variable pressure SEM and wish to do videotaping. The unit has a jack called "Video Out" that we use to obtain quick prints from a video printer. When we attempted to video tape from this jack, we obtained a poor quality image (dim, blurry, noisey, etc.). It appears that this might work but that some intervening device (signal booster) may be needed. Anyone have any experience with this? Many thanks.
############################################################################# John J. Bozzola, Ph.D., Director Center for Electron Microscopy Southern Illinois University Carbondale, IL 62901-4402 U.S.A. Phone: 618-453-3730 Fax: 618-453-2665 Email: bozzola-at-siu.edu Web: http://www.siu.edu/departments/shops/cem.html #############################################################################
On Friday, April 12, 1996 the annual Spring Workshop of the Oklahoma Microscopy Society (OMS) will be held in Room 246 in the Noble Research Center at Oklahoma State University (OSU), Stillwater, Oklahoma.
The main focus of the workshop is "Digital Imaging and Image Processing" and is being presented by Dr. David Bright, 1996 MSA Speaker and a Research Chemist from the National Institute of Standards and Technology in Gaithersburg, MD. The workshop will include some of the following topics:
Image Processing for Microscopists: Practice and Pitfalls Image Processing for the Macintosh: (free software available) What are digital images and why would one want to process them? Introduction to NIH Image and MacLispix Introduction to Image Processing -aspect ratio- "correction" for tilt or non square pixels -background subtraction and normalization -direction of gradient -euclidian distance map -mathematical morphology -linear hough transform
Other items of business:
(1) Student Best Electron Micrograph Contest: Don't forget to bring those prize-winning pictures with you to the workshop. Electron micrographs will be judged by attendees at the workshop. The winner will receive a $50.00 award and the micrograph will appear on the cover of the Fall 1996 OMS Newsletter. Second place winner will receive an award of $20.00.
(2) The Fall 1996 Technical Meeting in conjunction with the Oklahoma Academy of Science will be discussed. This year it will be held at the Oklahoma State University-OKC on Friday, November 8, 1996. Abstracts are due Monday, September 16, 1996.
(3) Final call for the submission of micrographs suitable for the new OMS t-shirt. Bring your entries to the spring workshop or mail them to Ginger Baker, Sec. before that date. This contest is not limited to students. The winner will receive a first edition of this T-shirt absolutely free. Remember that the T-shirts should be considered part of OMS' outreach efforts: they will be seen mostly by the non-microscopist public. In the now-famous words of our OMS president, "No hexamethylchickenwire lattice structures, please!".
(4) Discussion on the OMS web page expertly compiled and edited by Dr. Scott Russell, University of Oklahoma.
Address: http://www.uoknor.edu/electron/oms/
From this site, you can travel to a variety of pages for up-to-date microscopy news and information!
(5) Various meetings and shortcourses coming up during the year. Also, updates on the gradeschool microscopy kits and their use in the elementary classrooms.
Preregistration for the OMS Spring Workshop is $5.00 for members, $10.00 for student non-members, and $15.00 for non-members. After April 10 add a $5.00 late fee. On site registration begins at 8:30 a.m. Lunch is included in the fee.
Lunch: smoked turkey and brisket with barbecue sauce, cole slaw, baked beans, relish, wheat/white bread, and peach cobbler.
Parking: Lot 96: Each person driving to the workshop must have a visitor parking permit to hang in the vehicle with the designated lot number.
I have had a request from a materials science colleague to section a titanium alloy. He wants a blockface of several square mm and a thickness of 10 microns. Is this possible to do? Is there anyone out there who would be willing to do this for a fee? Thanks.
John Turek Purdue University Dept. Basic Medical Sciences
} I would appreciate any information on current user fees at university EM } facilities. My institution has requested a comparison of our fees with those } of other facilities. Thanks for your help.
At The Johns Hopkins Department of Earth & Planetary Sciences, we set the fee 11 years ago for in-house use at $35/hr for either the TEM or the microprobe. Add additional fees for operators (cost depending upon whether operator is student, staff, or faculty). TEM film is included in hourly rate, but polaroid film is not supplied for EMPA use and holey-C grids are charged for TEM use. Time starts when filament is turned on. This includes calibration time for the EMPA.
Cheers,
Kenneth JT Livi Department of Earth and Planetary Sciences 34th and Charles Streets The Johns Hopkins University Baltimore, Maryland 21218 (410) 516-8342 (voice) (410) 516-7933 (fax) klivi-at-jhu.edu (e-mail)
At 10:12 AM 3/7/96 -0600, you wrote: } The best processor out there is the Mohrpro. We have been using one } for about 8 years and recommend it when ever we can. It is easy to } use and maintain, 2 min. dry to dry, permanent (not stabilized) and } uses RC paper and Kodak Fixer.
I must respectfully disagree. I was personally responsible for implementing both an Ilford 2150 and a Mohr Pro 8" Model in a multiuser lab. While I liked the Mohr and our users were mostly happy with it, from an ease of use and maint. point of view, I would have to go with the Ilford.
The 2150 ran flawlessly for several years, then needed minor (in-house) maintenance - float sensors went bad. It will process up to 20" wide sheet, in 90 secs. dry-dry, and the rollers stay submerged in the chemistry, which seems to keep them cleaner (no dried on chemicals to deal with).
The Mohr has some rollers partially exposed, which often was a source of roller marks, and were more difficult to clean. We used it primarily for negatives (TEM & SEM). It performed well, just not as smoothly as the Ilford.
Overall, I had very good support from both Ilford and Mohr (though neither produced problems that really tested the waters). I would recommend both, but for different apps - Ilford for RC Prints (does not do film), and the Mohr for negs. With the caveat that the Mohr will require more hands-on time.
JCL ========================================================= James C. Long Manager/Materials Analysis Lab Electrosource, Inc. 512-445-6606 jlong-at-electrosource.com
I am looking for a used set of cryo accesssory for Reichert Ultracut E microtome. If anyone has one and willing to sell, please contact me directly. Thank you.
Ann Fook Yang e-mail: Yanga-at-em.agr.ca Phone: 613-759-1638
We just went through the problem of trying to calibrate a heating device for TEM specimens. In the process we made use of some very fine thermocouples which we found can be purchased at a very reasonable price from the Cole Parmer Company (7425 N. Oak Park Ave., Chicago, IL 60648; 800-323-4340). These are bare wire thermocouples (which means they are very flexible and easy to get into awkward places), and they are available in wire diameters from 0.03" down to 0.005", and in several combinations of wires for different temp ranges and applications.
Microscopy members, I have been in correspondence with Jordi Marti. He has been having problems with email, and has asked me to broadcast that the ISO summary (which was already posted) did not make it to the system. It will be reposted as soon as problems are solved (a few days) Prof.Walter A.Mannheimer Metallurgy and Materials Engineering Federal University of Rio de Janeiro POBox 68505 21945 Rio de Janeiro Brazil Voice 5521 280-7443 (Dept.office) 5521 590-0579 (direct) Fax 5521 290-6626 Email: wamann-at-metalmat.ufrj.br
Dear Wil: I read your e-mail message about heater calibration with interest. You might like to seek out a paper some colleagues and I wrote about 20 years ago Scanning Electron Microscopy 1976 Part I pages 83-90 in which we describe how we fabricated some very thin film thermocouples for use in the SEM. The women who did the work was extraordinarily patient and very nimble with her fingers. They worked, and showed us that a frozen sample kept at ca. 150K did not heat up when irradiated with the electron beam.
Can anyone refer me to a reliable independent SEM service company that can repair an S200? I'm in the Phoenix area, so a company near here or the west coast would be desirable.
********************************************************** Jake Schaper ASIC Product Analysis Lab Motorola, Inc. 1300 N. Alma School Rd. Chandler, Arizona 85224 Mail Drop CH240 Phone 602-814-4756 **********************************************************
We are searching an used SEM for received it in donation .The SEM will be used for Research and Education in our School. Please, we need know the model, year of fabrication, possibility of work, and other information available. We pay all the costs of shipmen. Please, contact me for any questions.
E-mail : RNBALDUC-at-arcide.edu.ar
Address:
Fernando Balducci Laboratory of Electron Microscopy School of Bioengineering. National University of Entre Rios. C.C 57 Suc 3 Parana - Entre Rios Argentina.
I have an interesting problem that I hope someone has some insight on. I have a light element EDS detector attached to a Philips 420 Supertwin, and processed pulses are analyzed by the DTSA/4Pi/Mac system. I have observed an unusual phenomenon that is interfering with our ability to model the shapes of characteristic peaks and backgrounds. All the peaks exhibit low-energy tails (LETs) that come and go depending upon a few parameters. They are:
(1) After a day of the detector bias being on but no X-rays enetering the crystal, the first spectrum of that day will have large LETs (} 3% of total peak area) that decrease with further exposure to X-rays. (2) LETs can be reduced if the bias is turned off for some time, but they will reappear after a day. (3) Conditioning the crystal only effects LETs as would turning off the bias. (4) Previous detectors have not shown as severe LET problems. (5) The amount of LETs can be reduced by lowering accelerating keV. (6) Every single component of this system has been replaced, including the crystal (3 times), but excluding the Mac computer and the TEM. This has had no effect. (7) There appears to be no problems related to ground-loops.
Observations 1 through 4 suggest a problem with the current detector system, but 5 and 6 may imply a problem with the scope alignment. Has anyone experienced similar problems with their detector? I have heard rumors that this has appeared on other TEMs, but is not a problem on SEMs and EMPs.
Many thanks to anyone with ideas.
Kenneth JT Livi Department of Earth and Planetary Sciences 34th and Charles Streets The Johns Hopkins University Baltimore, Maryland 21218 (410) 516-8342 (voice) (410) 516-7933 (fax) klivi-at-jhu.edu (e-mail)
We would like to request that if someone has an old EDS unit that will do qualitative analyses and hook up to a PGT detector, we'd be interested in looking at it and possibly purchasing. We don't need fancy bells, whistles, image analysis, dot-mapping ect. Just a basic unit that will take an x-ray spectrum and do some simple analyses.
The names/contact info. of reliable used equipment vendors would also be of assistance.
Thanks,
-Kirk _____________________________________________ Kirk Rogers krogers-at-materials.ecn.purdue.edu OR kirk.a.rogers.1-at-purdue.edu Purdue University, School of Materials Science and Engineering, 1289 MSEE building, W. Lafayette, IN 47907-1289 OFFICE: 317-494-8751 FAX: 317-494-1204 http://materials.ecn.purdue.edu/~krogers
I have placed a picture of a flat-wedge of optically transmitting (100) silicon on a web page: the image has thickness scale bars from 0 to 10 micrometers. This isn't an ideal sample: for example, there are no interference fringes visible in the submicron regime. However, it should serve as a starting reference. Please let me know what you think. The image is at
http://www.owlnet.rice.edu:80/~dlc/silicon.html
I am considering making a higher angle wedge hoping to preserve more of the thin region and also taking an image under a Na lamp as advised by Ron Anderson. Other suggestions are welcome as would other images, particularly of other orientation.
Daniel L. Callahan Department of Mechanical Engg. and Materials Science Rice University dlc-at-owlnet.rice.edu
Hi Everyone: I'm working on some projects using vitreous ice cryomicroscopy and would like to be able to determine the thickness of final ice layers on holey films. In general, I've heard of a couple of methods: 1) Burn a small hole, tilt, photograph; or 2) relative density by densitometry of negatives. I would be appreciative to receive details and references on these methods or other methods being used. What sort of accuracy should one expect from the various methods? Thanks for your help. Don Gantz Boston Univ. Med School e-Mail: gantz-at-med-biophd.bu.edu
Thanks to all who sent helpful suggestions regarding our inability to videotape from our S2460N variable pressure SEM. The problem was caused by a bad video input on our new video recorder (same brand as the microscope, oddly enough). When we used a different recorder, things worked beautifully. We're smilin' again.
############################################################################# John J. Bozzola, Ph.D., Director Center for Electron Microscopy Southern Illinois University Carbondale, IL 62901-4402 U.S.A. Phone: 618-453-3730 Fax: 618-453-2665 Email: bozzola-at-siu.edu Web: http://www.siu.edu/departments/shops/cem.html #############################################################################
Dear Listserver readers, We are a university EM unit primarily concerned with teaching and supporting research across many disciplines. The local hospital have a technician based in our Unit who processes their diagnostic cases for EM. Recently it came to my attention that he had processed a brain biopsy from a suspected Creutzfeldt-Jakob disease patient. What really concerned me was that : 1) We had not been informed that the sample was in the lab 2) The hospital technician did not appear to be aware of the high-risk nature of the disease.
This incident has highlighted two areas of concern in our Unit. Firstly we have no mechanism in place that requires the hospital to inform us when high risk biohazards come through our lab. Secondly, our mechanisms for dealing with "high risk" samples and disposing of such material need to be re-evaluated and improved.
My questions are:
How do other labs that are have high risk samples going through them monitor the risk of the samples coming into the lab?
How do other labs handle and dispose of high risk biohazards?
Allan Mitchell Richard Easingwood
Richard Easingwood South Campus Electron Microscope Unit School of Medical Sciences University of Otago PO Box 913 Dunedin NEW ZEALAND
Dear Amy and others, Good question. I have been looking (via the Current Contents for Physics and Chemistry) for TEM studies on historical art and archeological material for more than five years now and have never found any decent reference. It's always SEM. Of course the fact that for TEM you'd have to destroy part of the object is a serious problem. Nevertheless, as small quantities can be sufficient I agree that it should be considered. Could you please inform the group of the reactions that you get (maybe by posting a compilation after a few weeks). Thanks a lot. Nick Schryvers
Thanks Jonathan for agreeing to be the invited speaker on March 29. Copies of the notice are on their way to you. The title,"Extremophiles - organisms at the extremes" is intriguing. By the way can you explain for us what the CMB is and does? If you need info on how to get to McCrone Research Institute, I will send it to you.
To: Microscopy ListSer {Microscopy-at-MSA.Microscopy.Com} cc: BLAZEY --ENDVM1 Blazey, W.C. EMMI --ENDVM5 F. Emmi WAITKUS --ENDVM1 Waitkus, C. CONROW --ENDVM5 K.M. (Karen) Conro
We're proud of our capabilities, but near 10 micron thickness controlled over several mm is beyond our metallographic sectioning capability and our microtome, both! We could work with you on an experimental basis, but your goal is way out there.
{ } { { } } ===========} Dave King { { { } } } ------------------------------------------------------ { { { {
--Boundary (ID g1sXwhSkJTbyydWcZFNosA) Content-type: TEXT/PLAIN Content-transfer-encoding: QUOTED-PRINTABLE
I'm guesing by Nestor's test message that this didn't get through the= first=20 time, so I'll try again. Apologies to any who are receiving this for = a second=20 time.
I received a few requests for details on my electroless Ni route for= =20 fibre/powder preparation for the TEM. So here, culled from the lit., = is the=20 method I've settled on:
I'll start with the technique I use for electroless coating, then giv= e a few=20 references for electroplating, and then the method I use. For ceramic= powders I=20 I recommend electroless followed by electroplating.
Solution A: Add 0.1 gm SnCl2 to 100ml water pH adjusted to 2 with= HCl. (sensitising) Use at rm temp.
Solution B: Dilute 2mls of a 5% PdCl2 solution to 100 ml. (activator) Use at rm temp.
Solution C: Commercial Eleectroless solution made by HARSHAW. Ava= ilable=20 through Engelhard technologies (416) 821 - 3325. Comes in 3 parts Alpha 103 A-MB, Alpha 103 A-2, and= =20 Alpha 103 A-1 replenisher. =20
Make up 100 mls by adding 20 ml A-MB, and 7.5 ml A-2,= and=20 bringing up to 100 ml with D/I H2O. The replenisher = you will need if you use up all of the Ni, follow the destruct= ions=20 given by Harshaw for gauging this, and replenishing.
This solution is used at 90 - 95=F8C.
Proc: Add aprox 1cc powder to solution A with stirring. Af= ter=20 2 min. filter and rinse the cake with D/I H2O. Place= the=20 filter cake and paper in solution B with stirring. A= fter 2 min filter the sol and rinse the cake with D/I H2O.
Prepare a slurry by scraping the cake from the paper = and=20 adding no more than 10ml H2O. Pour this into solutio= n C, with stirring, allow to plate for aprox. 1 min, then = filter=20 and wash the powder.
And there you go.
Next, you incorporate the powder into a Eplated Ni sheet in your favo= rite plating bath (I use 80 ml Ni Sulphamate + 120 ml D/I H2O + 10 gm Bori= c Acid + 1gm Nickel Chloride, + 5 drops Hydrogen Peroxide).=20
I use a stainless steel beaker, an "L" shaped cathode to drop the pod= er on,=20 a high purity Ni anode, heat the sol to 50=F8C, and plate at 200 -300= mA (2.5V) DC. You need to plate a thin film(20 min), add just enough powder to= =20 get an even dispersion, and then let it plate away for 3 to 4 hrs. T= his=20 should result in a film somewhat in excess of 200=E6m.
A few key references:
S.D. Kirchoff and J. Y. Adkins, Metallography, 20 (1987) 75-87.
M.M. Morra, J. M. Morra, and R.R. Biederman, Materials SCiaence and E= ngineering, A124 (1990) 55-64
R. Baumann and M. J. Couper, Prakt. Met. 23 (1986) 140 - 148
R. D. Field and H. L. Fraser, Met. Trans. 9A, (1978) 131 - 134.
############################################################### # # # Don Steele STEELE-at-KRDC.INT.ALCAN.CA # # ALCAN INTERNATIONAL # # Kingston Research and Development Center # # P. O. Box 8400 # # Kingston, Ontario Canada K7L 5L9 # # # ###############################################################
} Recently it came to my attention that he had processed a brain biopsy from } a suspected Creutzfeldt-Jakob disease patient. What really concerned me was } that : } 1) We had not been informed that the sample was in the lab } 2) The hospital technician did not appear to be aware of the high-risk } nature of the disease. } } My questions are: } } How do other labs that are have high risk samples going through them } monitor the risk of the samples coming into the lab? } } How do other labs handle and dispose of high risk biohazards? } Dear Allan & Richard, Working at a state health department gives us a leg up on such things. There are procedures in place for proper notification, handling & disposal. We know what samples are being brought in for examination, and since it is usually a physician who brings in any potentially danger- ous specimens, we are made aware of any potential hazards. Usually, the specimen has already been fixed, stained and embedded, which eliminates the hazard. There are containers all around our lab for the disposal of biohazards, and the safety office is responsible for their proper dispo- sal. Having the hospital technician give you a list of specimens and their condition (tissue, blocks, sections, etc.) before bringing them in would be an obvious step. I can think of rationalizations for not doing this, but maybe you can insist. If you have a safety office, by all means get them involved. Good luck. Yours, Bill Tivol
The new Sony printers UP5600 will take PAL or NTSC signals - we handle a full line - also you could use a frame grabber from a variety of manufactures that take PAL in and let you output NTSC and use the existing printer. We carry all these products, please call if you require further assistance.
Scott E. Berman Advanced Imaging Concepts Princeton, NJ Phone(908) 274-1877 x26 Fax(908) 274-1974
For those of you waiting for the ISO summary, Jordi Marti (at Allied Signal) has been having problems with his E-Mail (he can't send mail out) for the past two weeks. The summary will be mailed as soon as the E-Mail problems are solved.
The above message was posted by Evex Analytical as a courtesy for Jordi Marti, Allied Signal
Dept. of Medical Physiology Inst. of Medical Biology University of Tromsoe N-9037 Tromsoe Phone : +47 77 67 45 48 or +47 77 64 46 96 Fax : +47 77 64 54 40
Fellow microscopists, I have to fix some algae (Cyanidium sp.) that are growing at pH 1.8 for TEM with and without Ruthenium red, to stain extracellular material. From what I have read glutaraldehyde fixes poorly at low pH's and conventional fixative buffers do not work below pH 4 (P04). Also,they can not tell me what the growth media is. I thought I could add straight glutaraldehyde and then bring up the pH slowly with NaOH or just rinse and fix normally under the assumption that the ultrastructure will not be altered, initially, by a higher pH. Does anyone have any experience with similar organisms or know of any references that may guide me? I guess I am inclined to try several approaches, but as is often the case the person has very limited funds and I was hoping to use someone elses experience (experiments) to reduce time and labor. Thanks in advanced, Hank Adams EML, NMSU Las Cruces, NM hadams-at-nmsu.edu
Perhaps you can help me with a personal dilemma; I'm about to purchase a microscope and I've boiled the choice down to either a Leica Galen III or a Carl Zeiss Standard 20. The Leica has more bells and whistles than the Zeiss, and for the setup I'm thinking of it's also about $1000 cheaper than the Zeiss. I have not used either and am 500 miles from the closest demo unit. In your personal opinion would I be better off, in the long-run, going with the Zeiss? Is one more comfortable to use than the other in terms of fatigue? How would YOU compare these two microscopes generally?
Peter Slocombe Washington State University slocombe-at-wsunix.wsu.edu
Dear Peter Here at RBGE we have both Leica and Zeiss models, although we don't have the Galen III. Traditionally we always had Leica models but when they changed to infinity corrected optics, traditions didn't help any more, and so we started looking afresh with an unbiased (obviously infinity corrected) view. Our choice was Zeiss, partly because of better optics although some of us find the Zeiss microscopes slightly less user friendly, and I believe the Leica optics have improved now. Here is my suggestion: You may live 500 miles away from a demo unit, but why don't you ask both Leica and Zeiss to leave the microscope with you for a week or so; this is the only way you can really judge for yourself which one you would prefer for your own specimens. The physics of microscopy are pretty well sussed out these days, so what you want to pay extra for is durability (less trimmings normally means less trouble) and ease of use (more trimmings sometimes help).
Cheers
Stephan
Yours sincerely
Dr Stephan Helfer, SSO Mycologist / Plant Pathologist
Royal Botanic Garden Edinburgh, Inverleith Row, EDINBURGH EH3 5LR, Scotland UK
http://www.rbge.org.uk
phone: +44 (0)131 552 7171 ext 280 or +44 (0)131 459 0446-280 (direct digital VoiceMail line) fax: +44 (0)131 552 0382 ============================ 1896 * BRITISH * MYCOLOGICAL * SOCIETY 1996 A century of fungal science ============================
} Has anyone heard of the slow viruses that can survive gluteraldehyde fixation?
The infectious agents that are able to survive fixation are the prions. These agents contain protein only...no nucleic acid. Among these are Creutzfeld-Jakob, scrapie, kuru, Gersten-Straussler-Schenker, BSE (mad cow disease) and apparently some forms of Alzheimers.
It is known that formaldehyde does not inactivate them, not surprising since formaldehyde is one of the least cross-linking of all fixatives, so far as proteins are concerned. My understanding is that glut. does inactivate them, but don't take my word for it.
} On a similar note, there was an MSA-sponsored speaker a couple of years ago who } was encouraging EM labs to make money by offering virus identification sevices } to medical centers. The preparation protocol included advice to accept unfixed } (ie. infectious), unidentified material and prepare negative stained samples on } the bench in the laboratory.
We are a CLIA and State certified human clinical laboratory, licensed for negative-stain virus identification. This has always been part of our function as a veterinary EM lab, and for about 5 years we have offered it as a service to the community. We do make a considerable amount of money from it. We accept samples only from certain reference laboratories and practitioners and these are only stool samples, mostly (} 95%) from infantile diarrheas. Our submission form has an entry for any unusual precautions that must be taken (ie HIV, hepatitis, etc). Our understanding with our clients is that we don't process such samples. W. L. Steffens, Ph.D
Dept. of Veterinary Pathology College of Veterinary Medicine University of Georgia Athens, GA 30602 STEFFENS.B-at-CALC.VET.UGA.EDU Voice: (706) 542-5536 FAX: (706) 542=5828
Title: Biological Science Technician (Plants) Lab: USDA-ARS, Salinas, CA
The USDA-ARS is seeking a biological science technician (plants) (GS-404-7, 8, or 9) for the Crop Improvement and Protection Research Unit in Salinas, CA. The incumbent will share responsibilities in electron microscopy of plant virus infections for ultrastructural characteristics. The incumbent will also assist in research involving molecular, serological, and biological studies of several plant viruses infecting sugarbeet and vegetables. Candidate must have a knowledge of electron microscopy, plant virology, and knowledge of microbiological techniques. Must be a U.S. citizen. Bachelors degree is desirable. Salary is commensurate with experience ($24,610-39,140 per annum). For information regarding research program contact Gail C. Wisler or James E. Duffus (408)755-2835. For information regarding application procedures/forms contact Tom Nelson (408)755-2810. Applications must be postmarked by May 6, 1996. The USDA is an equal opportunity employer.
I find it hard to believe that no such scopes have been *sold* closer than 500 miles away. Ask your dealers, if they have sold such scopes, and if they will contact those people and see if they will be willing to have you inspect the scope.
Both companies make *excellent* products. At this quality, what feels right for *you* is very important. So is the *service* you get from your agent. Also check what scopes your facility has. The ability to "borrow" a specific "widget" down the hall should be weighed in the equation.
As another user suggested, the agent may be able to lend you a scope to try.
All these conversations should also give you a pretty good idea of how well you can work with the agent after the sale.
Good luck
Azriel Gorski, Head Optical Microscopy Laboratory Division of Identification and Forensic Science Israel Police
Does anyone have a protocol for microwave fixation of marine organisms? I am considering this technique for fixing some crustacean tissue (hepatopancreas), and a colleague of mine is considering microwave fixing marine algae.
Amy Aslamkhan University of Hawaii aslamkha-at-hawaii.edu
We are attempting to start a bio-physics program at Chicago State University. We need feed back as to the role of physics in biological sciences problem solving. What is the trend? Is it accurate to say that biology and physics are becoming intertwined ? I personally see a need for a greater understanding of physics when working with the newer technology in electron microscopy in biological science but what do you think?
Via: uk.ac.bbsrc; Mon, 18 Mar 1996 21:48:50 +0000 X400-Received: by /PRMD=UK.AC/ADMD= /C=GB/; Relayed; Mon, 18 Mar 1996 21:50:50 +0000 X400-Received: by mta arcs.bbsrc.ac.uk in /PRMD=UK.AC/ADMD= /C=GB/; Relayed; Mon, 18 Mar 1996 21:50:50 +0000 X400-Received: by mta LARS.MUAS in /PRMD=UK.AC/ADMD= /C=GB/; Relayed; Mon, 18 Mar 1996 21:46:48 +0000 X400-Received: by mta LARS in /PRMD=UK.AC/ADMD= /C=GB/; Relayed; Mon, 18 Mar 1996 21:47:34 +0000 X400-Received: by /PRMD=UK.AC/ADMD= /C=GB/; converted (ia5-text); Relayed; Mon, 18 Mar 1996 21:48:05 +0000 Return Requested)
} As far as I know Alzheimers disease is not infectous---- Where did you get your } information about Alzheimers disease?
This information is at least 4 years old and may not be correct. About 4 years ago (maybe a little longer) there was a lengthy review article in either New England Journal of Medicine or JAMA regarding the slow human neurological disorders. At that time, there were at least 5 recognized diseases that were classified as "Alzheimer" or "Alzheimer-like". One of these was suspected to be of prion origin, although by now it may be classified as something else.
One thing to consider if you are involved in studies involving human (or any mammalian) brain tissue is that in years past, prion diseases were commonly misdiagnosed as Alzheimers, and as humans (even pathologists) are not perfect, this still may be the case.
W. L. Steffens, Ph.D Dept. of Veterinary Pathology College of Veterinary Medicine University of Georgia Athens, GA 30602 STEFFENS.B-at-CALC.VET.UGA.EDU Voice: (706) 542-5536 FAX: (706) 542=5828
I have been working on a project with a dental grad student involving ultramicrotomy of undecalcified human teeth. The samples were prepared by slicing them into approx. 100um slices and then embedding them in Epon. They section just great but something happens when they're picked up on grids which causes just the dentin portion of the section to wrinkle. I use formvar coated 200 mesh copper grids, stabilized with carbon and glow discharged. I spread the sections out in the boat with chloroform (I have used xylene too) and have picked them up every differnt way I could think of. Is it my support film? Would a thicker section be desirable (sections are usually 90-95 nm)? It is only the dentin part of the section that has this problem, the Epon and composite resin reconstruction are perfectly flat. Does anybody have any suggestions?
Thanks in advance,
Jean Ross Central Microscopy Research Facility University of Iowa 85 EMRB Iowa City, IA 52242 (319) 335-8142 Web site: http://www.uiowa.edu/~cemrf
We are interested in trying some fluorescent in situ hybridisation on tissue sections. We would be using fetal sheep tissue and would be probing with riboprobes to growth factors and hormone receptors mainly.These probes generally work well (as far as any in situ does) when radioactively labelled, but we would be keen to get away from radioactive labels and would really like to try multiple labelling. It seems very difficult to find references describing the technique applied to tissue sections so any help, advice ,sources of information or contacts would be much appreciated.
Thanks for your time
Peter Smith AgResearch Wallaceville PO Box 40063 Upper Hutt New Zealand.
Dept. of Medical Physiology Inst. of Medical Biology University of Tromsoe N-9037 Tromsoe Phone : +47 77 67 45 48 or +47 77 64 46 96 Fax : +47 77 64 54 40
} This information is at least 4 years old and may not be correct. } About 4 years ago (maybe a little longer) there was a lengthy review } article in either New England Journal of Medicine or JAMA regarding } the slow human neurological disorders. At that time, there were at } least 5 recognized diseases that were classified as "Alzheimer" or } "Alzheimer-like". One of these was suspected to be of prion origin, } although by now it may be classified as something else. } } One thing to consider if you are involved in studies involving human } (or any mammalian) brain tissue is that in years past, prion diseases } were commonly misdiagnosed as Alzheimers, and as humans (even } pathologists) are not perfect, this still may be the case.
AD and prion diseases share the presence of amyloid precursor proteins (beta-PP, APrP) but they are distinct diseases, both starting at the synapse (beta-PP and PrP are proteins of the neuromuscular junction and CNS synapse). In some of the prion diseases prion amyloid plaques are seen together with paired helical filaments (NFT) making them "Alzheimer-like" as Budy writes.
********************************************************* Sverker Enestrom M.D., Ph.D. Department of Pathology University of Linkoping, Sweden Phone: +46 13 22 15 20 Fax: +46 13 13 22 57 *********************************************************
Amy- I don't know of any specific references to microwave fixation of crustacean tissue, but you might want to experiment with the method developed by Bill & Ruth Dewel (Appalachian State University) for fixation of tardigrades (Echiniscus viridissimus). Please contact me by e-mail for the methodology. General information on microwave fixation for TEM can be found at our WWW site (http://www.ebsciences.com/). Steven Slap, Vice-President 75767,640-at-compuserve.com
Greetings, My understanding was that you got prion diseases through eating contaminated samples, hopefully uncommon in the world's em prep rooms. Is there an airborne transmission route? Thanks, Tobias Baskin
Via: uk.ac.bbsrc; Tue, 19 Mar 1996 14:53:40 +0000 X400-Received: by /PRMD=UK.AC/ADMD= /C=GB/; Relayed; Tue, 19 Mar 1996 14:49:57 +0000 X400-Received: by mta arcs.bbsrc.ac.uk in /PRMD=UK.AC/ADMD= /C=GB/; Relayed; Tue, 19 Mar 1996 14:49:57 +0000 X400-Received: by mta LARS.MUAS in /PRMD=UK.AC/ADMD= /C=GB/; Relayed; Tue, 19 Mar 1996 14:47:06 +0000 X400-Received: by mta LARS in /PRMD=UK.AC/ADMD= /C=GB/; Relayed; Tue, 19 Mar 1996 14:47:09 +0000 X400-Received: by /PRMD=UK.AC/ADMD= /C=GB/; converted (ia5-text); Relayed; Tue, 19 Mar 1996 14:47:20 +0000 Return Requested)
Hello, I am currently doing TEM on sorghum leaves looking at the infection process of Colletotrichum graminicola. Sorghum leaves produce anthocyanidin phytoalexins in response to infection and I would like to locate these compounds under the TEM. I was wondering if anybody out there had or knew where I could get hold of antibodies to anthocyanidins, specifically apigeninidin and luteolinidin, or if anyone knew of any stains for EM which were specific to anthocyanidins.
Phill Wharton
*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-* Phillip Wharton IACR-Long Ashton Research Station Long Ashton Bristol BS18 9AH *-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*
} We are attempting to start a bio-physics program at Chicago State } University. We need feed back as to the role of physics in biological } sciences problem solving. What is the trend? Is it accurate to say } that biology and physics are becoming intertwined? I personally see a } need for a greater understanding of physics when working with the newer } technology in electron microscopy in biological science but what do you } think?
Dear Joyce, Physics has several roles in bio problem solving. There is the application of physical measurements (ultracentrifugation, calorimetry, diffraction to name just a few), the use of physics in understanding what happens to the specimen (radiation damage in EM, physical preparation techniques like microwaving & sectioning), and, perhaps most important, the basic viewpoint of physics can aid thinking about bio phenomena-- this has proved to be especially valuable to me. I found that my training in physics gave me the quantum-mechanical viewpoint from which to view enzyme-substrate interactions and molecular structure--which gave me in- sights that my colleagues not trained in physics did not have. I believe the trend in research is toward interdisciplinary studies, so physics, among other disciplines, is being applied more and more to other fields, including biology. I would not single out the bio-phys linkage as unique (or even one-way). I think that all disciplines are becomming more fluid with the boundaries getting fuzzier rather than that biology and physics specifically becomming intertwined. Understanding the principles of the instrumentation is always a plus, and the newer technology certainly relies on physical principles. On the other hand, the newer instrumentation is also becomming more user- friendly and computer-oriented. This means that the instruments can be operated merely by clicking on a few menus, and repair of a faulty in- strument usually means replacing a computer chip rather than disassembling, analysing which part is broken, making a new part and reassembling. At the latter level, less understanding of physics may be required to perform the experiment, but more required to understand it. Yours, Bill Tivol
In message {Pine.SV4.3.91.960318100758.25965A-100000-at-uhunix5} Amy G Aslamkhan writes: } Does anyone have a protocol for microwave fixation of marine organisms? } I am considering this technique for fixing some crustacean tissue } (hepatopancreas), and a colleague of mine is considering microwave fixing } marine algae. } } Amy Aslamkhan } University of Hawaii } aslamkha-at-hawaii.edu
Microwave fixation is an ideal method to preserve the soft tissue within a hard (calcified shell). References appropriate to your application follow:
Berg, C. J., and N. L. Adams. Microwave fixation of marine invertebrates. J Exp Mar Biol Ecol 74: 195-199, 1984.
Login GR, Dvorak AM. Methods of microwave fixation for microscopy. A review of research and clinical applications: 1970-1992. Prog Histochem Cytochem 1994;27/4: 1-127.
If you need further assistance or methodological details please do not hesitate to contact me by calling or sending a fax to the number at the end of this E-mail message.
Gary Login
Gary R. Login, D.M.D., D.M.Sc. Assistant Professor of Oral Pathology Beth Israel Hospital Department of Pathology 330 Brookline Avenue Boston, Massachusetts, 02215
} My understanding was that you got prion diseases through eating } contaminated samples, hopefully uncommon in the world's em prep rooms. Is } there an airborne transmission route?
The review article that I described earlier notes some cases of prion disease in neurosurgeons who had performed procedures on known infected patients in years past. Not exactly iron-clad evidence of alternative transmissibility....but still.
This thread had gotten my interest on the subject piqued again. I'll see if I can dig out the original article.
W. L. Steffens, Ph.D Dept. of Veterinary Pathology College of Veterinary Medicine University of Georgia Athens, GA 30602 STEFFENS.B-at-CALC.VET.UGA.EDU Voice: (706) 542-5536 FAX: (706) 542=5828
I've come across a similar problem with epon embedded inner ear (cochlea). In my case, the soft tissue I need to look at is completely surrounded by decalcified bone. Several things I've tried seem to help with this problem: trim away the bone from the surface of the block before sectioning or increase infitration times, using a harder epon mix to try and reduce the difference in hardness that occurs in the block between pure epon, bone and soft tissue. I hope this helps. P.S. I've had this happen even on bare grids.
Karen Pawlowski Technician, UT Southwestern Medical Center PhD student, UT Dallas
} My understanding was that you got prion diseases through eating } contaminated samples, hopefully uncommon in the world's em prep rooms. Is } there an airborne transmission route?
The most common form of transmission for toxoplasma gondii is from eating cat feces but I am sure no-one would admit to eating that!
Paul Webster, Ph.D. Center for Cell Imaging Yale University School of Medicine
The University here has recently decided to unionize the staff. I was wondering if SEM, NMR, MS technicians are commonly unionized in the experiences of the group. We were hired into our designated positions and there really isn't a ladder to climb to higher positions or designations. Overall, I'm having a difficult time seeing the advantage of being part of a union although I'm fully aware of the price deducted from my salary. Is this a common practice in Canada or elsewhere?
I am using an In_lens Field Emission SEM to image various single crystals. Can anyone suggest a technique for measuring the angles between single crystal faces? (Since the IFESEM is an in-lens microscope use of a double detector system is impossible)
Is it possible to take secondary electron stereo pair images and measure the true crystal face angles?
Colin MacRae Scanning Probe Microscopy and Electron Microscopy Section
Commonwealth Scientific and Industrial Research Organisation. _--_|\ cmac-at-minerals.csiro.au Division of Minerals / \ +61 3 9647 0296 PO Box 124, Port Melbourne 3207 \_.--._/ fax 61 3 9646 3223 AUSTRALIA
EM units WWW site http://www.minerals.csiro.au/em-unit/
At 06:20 PM 3/19/96 -0500, you wrote: } } My understanding was that you got prion diseases through eating } } contaminated samples, hopefully uncommon in the world's em prep rooms. Is } } there an airborne transmission route? } } The most common form of transmission for toxoplasma gondii is from eating cat } feces but I am sure no-one would admit to eating that! } } } Personally I find a Snickers more satisfying. I have been told oth by my Vet and our OB/GYN that it is possible to contract the organism through the dust kicked up while kitty uses his litterbox not to mention when we have to change it. {} {} {} {} {} {} {} {} {} } {} {} {} {} {} {} {} {} {} {} {} Scott Whittaker Ph 352-392-1295 ICBR EM Core Lab Fax 352-846-0251 University of Florida e-mail sdw-at-biotech.ufl.edu www http://www.biotech.ufl.edu/~emcl/
I am emploted at an EM facility at the University of the North, South Africa. We have been running an EM course for postgraduate students for the past 3 years. It is part of a techniques course for fourth year students in the biological sciences, and serves as an introductory course. Following their training, many students include ultrastructural studies as part of their projects
I am quite eager to year of institutions that are involved in EM training. In particular, I am interested in course content, training facilities (in- cluding instruments), and whether or not such a course improved past students chances of employment or to get sponsorship for futher studies.
Leon Scott EM Unit Univ. of the North Private Bag X1106 Sovenga Republic of South Africa tel 27 152 2682957 fax 27 152 2683156/2682893
Dear Fellow Microscopists, I'm currently a biomedical photographic student at the Rochester Institute of Technology. I have a BS in Biology and I'm currently pursuing a second degree in Biomedical Photographic with a concentration in photomicrography. My career objective is to apply my knowledge in biology and biophotography to the documentation of medicine and the biological sciences. You can view my resume and some of my images in my web site at http://www.isc.rit.edu/~caa3045. The biomedical photographic Communication department at RIT has exposed me to many photography techniques. I am experience with many formats of films processes, black-and-white, and color printing, photomacrography, photomicrography, darkfield, brighfield, nomarski, phase-contrast, polarizing, Rheinberg, SEM, and fluorescence microscopy. Also, I have a great interest and hands on experience in digital photography and computer (Macintosh major area of strength). Also, I have a lot of experience with several immnunocytochesmistry techniques, and the Patch-clamp techniques. If you think I may contribute to your department send me an e-mail to "caa3045-at-rit.edu" or "almonte-at-medcolpa.edu" Thanks,
__________________________________________________________ Ciprian A. Almonte Rochester Institue of Technology Biomedical Photographic Communications Rochester, NY 14623-5603 Visit my web site at http://www.isc.rit.edu/~caa3045/ __________________________________________________________
Message-ID: {199603201324.IAA08945-at-IndyNet.indy.net} To: Colin MacRae {C.Macrae-at-minerals.csiro.au} , Microscopy list {microscopy-at-Sparc5.Microscopy.Com}
Quick question: are Eppendorfs air-tight enough to use as embedding capsules for LR White? I have cell pellets to embed and would rather not disrupt them if I can avoid doing it. Thanks! Tamara
The Laboratory of Electron Microscopy, School of Bioengineering,U.N.E.R Argentina, is look for SEM to received it in donation or a very low price. Only is required the technical information of your " work state", the model and year of construction. We pay all the costs of shipment Please, for any questions contact me in
Alternate-Recipient: allowed Auto-Forwarded: prohibited Content-Return: allowed Disclose-Recipients: prohibited Conversion: allowed Importance: normal Priority: normal Sensitivity: Company-Confidential
No plastic capsule or tube that I know of is air-tight enough for LR White.You may double emded with gelatin and get away with a plastic capsule. Kate Connolly
O.k. does anyone out there know where to get a vial of TMV (Tobbacco Mossaic Virus) from for use as a mag standard and particulate test specimen?
Carolina Biological is the only source I have come across but they sell it as a whole kit for learning about Plant viruses, and I just want the virus particles.
Message-Id: {199603201527.LAA19788-at-Snoopy.UCIS.Dal.Ca} Comments: Authenticated sender is {RMACKAY-at-ac.dal.ca}
A colleague and I were discussing microprobe analysis of garnets and he remarked that their analysis of Almandine often produce high totals, a phenomenon I have also observed with high Fe garnets. I know that others have observed this as well but has anyone come up with an explanation ?
Bob MacKay Robert MacKay Department of Earth Sciences Dalhousie University Halifax, Nova Scotia, Canada B3H 3J5 Tel: 902 494-7087 e-mail rmackay-at-ac.dal.ca
For those of us following this thread, the following statement issued today by USDA-APHIS will be of interest.
} The British Ministry of Health and Agriculture have announced today that } they may have associated CJD to BSE - an announcement was made to the } House of Commons today.
I suppose in the future, we may be refusing to allow bovine tissues into our labs.
-=W.L. Steffens=- Department of Veterinary Pathology College of Veterinary Medicine University of Georgia
Post doctoral positions are available at the Naval Research Laboratory in the area of materials characterization of superconductors for U.S. citizens with a Ph.D. in metallurgy, physics or chemistry. Techniques utilized are TEM, SEM, SAM and optical microscopy. Most modern instruments in these fields such as a new CM-30 TEM are available including access to large scale computational facilities Previous experience in superconductivity is not required but expertise in transmission electron microscopy is necessary. For further details, please contact: Dr. Chandra Pande Code 6320 tel:(202).767-2744 Naval Research Laboratory fax:(202) 767-2623 Washington, DC 20375-5343 e-mail:pande-at-anvil.nrl.navy.mil
Chandra S.Pande email: pande-at-anvil.nrl.navy.mil phone: (202) 767-2744 fax: (202) 767-2623 Mailing address: Code 6325 Naval Research Lab Washington DC 20375 USA
On Wed, 20 Mar 1996, Tamara Howard in Cold Spring Harbor Laboratory wrote:
} Quick question: are Eppendorfs air-tight enough to use as embedding } capsules for LR White? I have cell pellets to embed and would rather not } disrupt them if I can avoid doing it. } Thanks! } Tamara }
We routinely embed material in eppendorfs. They work fine.
Jay Jerome ************************************************************** * aka: W. Gray Jerome * * Dept. of Pathology * * Bowman Gray School of Medicine of Wake Forest University * * Medical Center Blvd * * Winston-Salem, NC 27157-1092 * * 910-716-4972 * * jjerome-at-bgsm.edu * **************************************************************
} Date: Wed, 20 Mar 1996 08:37:18 -0600 (CST) } Precedence: bulk } From: DrJohnRuss-at-aol.com } To: Multiple recipients of list {nih-image-at-Soils.Umn.EDU} } Subject: Image Analysis Short Course } X-Comment: NIH Image Distribution List } } Apologies to those who have already read this. I posted it several months ago } but from the number of phone and e-mail inquiries it seems worthwhile to put } it up again. } } The intensive short course on image analysis and stereology that North } Carolina State University has presented for many years will be offered three } times in 1996, twice in Raleigh, NC (May 16-18 and May 20-22) and once in } Denmark (June 12-15). The textbook for the course is "The Image Processing } Handbook, 2nd Edition" by John C. Russ. Attendees also receive a CD-ROM } (usable on both Macintosh and Windows) containing many images and a } comprehensive set of plug-in modules for Adobe Photoshop that implement all } of the various processing and measurement algorithms discussed in the book, } with a detailed graphic step-by-step tutorial on disk. The faculty for the } course include John Russ (NCSU), Robert Dehoff (Univ. of Florida) and } Jeanette Norden (Vanderbilt Univ). This is a good opportunity to learn the } fundamentals of quantitative image analysis as well as get answers to } specific problems, understand how and when to use various methods and } algorithms, and how to interpret results in a variety of fields including } materials science, geology, biology and medicine, forensics, food science, } etc. The course includes hands-on laboratories and the opportunity to work } with attendees' images. } } For more details, you may request the printed brochure by calling Belinda } Niedwick at NCSU Dept. of Continuing and Professional Education, } 919-515-8185. There is also a web site with detailed course description and } on-line registration at http://vims.ncsu.edu/matsci/IPCourse.html } } Thanks for your interest. } John Russ }
-Kirk _____________________________________________ Kirk Rogers krogers-at-materials.ecn.purdue.edu OR kirk.a.rogers.1-at-purdue.edu Purdue University, School of Materials Science and Engineering, 1289 MSEE building, W. Lafayette, IN 47907-1289 OFFICE: 317-494-8751 FAX: 317-494-1204 http://materials.ecn.purdue.edu/~krogers
} Quick question: are Eppendorfs air-tight enough to use as embedding } capsules for LR White? I have cell pellets to embed and would rather not } disrupt them if I can avoid doing it. } Thanks! } Tamara } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } }
Yes, as long as you nearly fill the tube with resin to exclude as much air as possible -at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at- Greg Erdos Phone: 352-392-1295 Scientific Director, ICBR Electron Microscopy Core Lab 218 Carr Hall Fax: 352-846-0251 University of Florida E-mail: gwe-at-biotech.ufl.edu Gainesville, FL 32611 -at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
} Quick question: are Eppendorfs air-tight enough to use as embedding } capsules for LR White? I have cell pellets to embed and would rather not } disrupt them if I can avoid doing it. } Thanks! } Tamara
They're fine, though as always happens there will possibly be a squishy bit at the top of the tube where the LRW hasn't hardened.
Diana van Driel Dept Ophthalmology Sydney University AUSTRALIA 2006
} Could someone direct me to the website that has jobs/resume postings? } Thanks, Shelley Landon KaurinDear Shelley:
The new search engine, ALTA VISTA, is good for this type of thing. In addition to a vast text index of web pages, it can also search usenet-including the newsgroups where most jobs are posted. Address is http://www.altavista.digital.com; if you have problems (some browsers return a blank screen), we have a direct link to it from our "Links" page (http://www.tiac.net/users/everlast/nano/Links.html, in the "Internet and WWW directories" section. Try a simple query with something like "Jobs offered microscopy" with the search field set to "Usenet".
On the Web, try Career Mosaic (http://www.careermosaic.com), which indexes many of the jobs offered newsgroups. This has links to places to post resumes as well.
Regards,
Rick Powell
***************************************************************** * NANOPROBES, Incorporated * * 25 East Loop Road, Suite 124, Stony Brook, NY 11790-3350, USA * * * * http://www.tiac.net/users/everlast/nano/home.html * *****************************************************************
} I am using an In_lens Field Emission SEM to image various single crystals. } Can anyone suggest a technique for measuring the angles between single } crystal faces? (Since the IFESEM is an in-lens microscope use of a double } detector system is impossible) } } Is it possible to take secondary electron stereo pair images and measure the } true crystal face angles? } Dear Colin, It is certainly possible to reconstruct the 3D volume from a stereo pair, but that is not very accurate and you would need the appro- priate software to perform the calculation. If you have a double-tilt or tilt-rotation specimen holder and can manipulate the specimen until one of the faces is normal to the beam, then re-orient so that the second face is normal to the beam, then you can get the angle between the faces more accu- rately if you can record angular positions for the two orientations. Yours, Bill Tivol
If you can sputter coat the sample with gold, you can probably get them to pump down well enough to look at them in the SEM. We used to look at tar sands samples with a Cambridge S250 turbo SEM after they were placed in a vacuum dessicator for a few days to remove the light fraction of the oil. However you will inevitably get some contamination of the column when the beam heats up the oil, especially during x-ray analysis, so plan to do a column clean shortly after running the oil samples. The best way to run these types of samples is with a cryo stage. Freeze the little buggers so they can't mess up your aperatures!!!
} I have an Amray 1600 Turbo SEM, I have a question about a sample } } that I would like to observe. The sample is an oil that a plant } } uses for a dosing machine. There are visible spots in the oil that we } } suspect may be Hg or Na, or the oil may be a synthetic oil, one that was } } not supposed to be used in the first place. I was asked if it is possible } } to examine a smear of the oil under SEM/EDX and identify the spots in it, } } but I'm thinking no because of the wet oil contaminating my sample chamber, } } and also the temperature of the electron beam may volatile the mercury. } } Is it not possible to observe this oil with my instrument? Are there any } } suggestions that I may follow? } } } Mark Darus } General Electric Co. } } Darus-at-cle.dnet.ge.com
Cheers :) George Department of Earth and Atmospheric Sciences University of Alberta Edmonton, Alberta T6G 2E3 Canada ph: 403-492-5746 fax: 403-492-2030
Please put my name onto your mailing list (microscopy news). Thanks in advance, Bela Pecz 21st March 1996
----------------------------------------- Dr. Bela Pecz Research Institute for Technical Physics H-1325 Budapest, POBox 76 Hungary phone: 36-1-1698-961 fax: 36-1-1698-037 E-Mail: pecz-at-falcon.mufi.hu -----------------------------------------
Dear Tamara I have used LR White in three sizes of Eppendorfs (0.5, 1 & 2ml or thereabouts). Air did not seem to be a problem, but the plastic used in the tubes must have reacted with the resin. The smallest size tube resulted in strangely shaped blocks, the bigger ones seemed ok. I didn't persue the matter, but I could imagine that coating the inside of the tubes with gelatine might help preventing any reaction (I also used gelatine capsules inside Eppendorfs which worked very well). Hope this helps
Yours sincerely
Dr Stephan Helfer, SSO Mycologist / Plant Pathologist
Royal Botanic Garden Edinburgh, Inverleith Row, EDINBURGH EH3 5LR, Scotland UK
http://www.rbge.org.uk
phone: +44 (0)131 552 7171 ext 280 or +44 (0)131 459 0446-280 (direct digital VoiceMail line) fax: +44 (0)131 552 0382 ============================ 1896 * BRITISH * MYCOLOGICAL * SOCIETY 1996 A century of fungal science ============================
I would filter the sample trough a membrane filter (Millipore?) and wash with a suitable solvent (n=hexane?) to eliminate the oil. Then dry and carbom coat. The residue in the membrane should be easily observed in SEM. Dagoberto Rodriguez -------------------------
} I have an Amray 1600 Turbo SEM, I have a question about a sample } } that I would like to observe. The sample is an oil that a plant } } uses for a dosing machine. There are visible spots in the oil that we } } suspect may be Hg or Na, or the oil may be a synthetic oil, one that was } } not supposed to be used in the first place. I was asked if it is possible } } to examine a smear of the oil under SEM/EDX and identify the spots in it, } } but I'm thinking no because of the wet oil contaminating my sample chamber, } } and also the temperature of the electron beam may volatile the mercury. } } Is it not possible to observe this oil with my instrument? Are there any } } suggestions that I may follow? } } } Mark Darus } General Electric Co. } } Darus-at-cle.dnet.ge.com } }
Thanks to everyone who responded to my call for help. In case anyone is interested, the replies split about 70/30 in favor of Eppendorfs, but several people said they'd never been able to get them to work for LRW. There were a few who suggested using gelatin capsules with the Eppendorfs - as additional air barriers. I'll see how the plain tubes work, then try the elaborate set-ups as needed. Thanks again! Tamara
On Wed, 20 Mar 1996, Tamara Howard in Cold Spring Harbor Laboratory wrote:
} Quick question: are Eppendorfs air-tight enough to use as embedding } capsules for LR White? I have cell pellets to embed and would rather not } disrupt them if I can avoid doing it. } Thanks! } Tamara Tamara: it works ok, but removing them from the eppenddorfs can be tricky sometimes. Also, if your pellets are too dense, poor infiltration and polymerization can be a problem. Why not embed them in agar first in the tubes then transfer them..} } } Hank Adams, NM
I am startint to look at tapeworms in the Trypanorhynch group and was wondering if someone has a suggestion for a good fix for the preservation of "actin". I normally use 2.5% glut in cacodylate buffer. Will this be alright to use? I greatly appreciate any help.
I read that you run a list server for microscopy. I believe Larry Thomas from our lab subscribed but now that he has retired I would like to begin receiving this service. Do I need to do anything?
We recently acquired a variable pressure sem (Hitachi S3200N) with a Fullam cold-stage, a researcher wants to image fresh leaves and is particularily interested in the status (open vs closed) of the stomates. Does anyone have any suggestions as to temp, pressure, Kv, beam current, etc?
} Thanks to everyone who responded to my call for help. In case anyone is } interested, the replies split about 70/30 in favor of Eppendorfs, but } several people said they'd never been able to get them to work for LRW. [snip] Just wanted to point out that the term "Eppendorf tube" is a bit like "whiskey", understood instantly, but in fact refering to a wide range of product. So, "ep tubes" are made by gazillions of companies and presumably out of, well, dozens, of different kinds of plastics. Might be worth finding out from someone in the successful camp exactly where they bought their ep tubes.
Here is a summary of the processor discussion. Contact the person who posed the question to get the responses that didn't get posted to the list. In the future, we maintain a web site in which we archive most of the discussions for future reference. Go to the web site listed at the bottom of this page and look in "Tips & Tricks". We have added a new search feature and would welcome any input.
At 01:29 PM 3/20/96 -0500, you wrote: } Not long ago, photo processors were discussed by this group. Of course } as soon as the last message on this subject went into my trash, our } negative processor broke and is beyond repair. It is a vintage processor } by Hope (Model 152 B&W13-10), and spare parts are not anymore available. } Also it was not always obvious in the discussion, if the subject was } negative or positive processors. Please, if anyone has a summary of the } results, I would appreciate to receive a copy by e-mail. Of course, I } welcome any new information as well. } } Thanks very much, } } Hasso Weiland } Alcoa Technical Cneter } Alcoa Center, PA 15069 } --------------------------------------------- Considering that photography is still so important to our profession, it is likely that in this group are a few critical individuals with personal experiencee using dry-to-dry black and white photographic processors. Would anyone care to recommend a counter-top processor that they are happy with? I wouldn't object to knowing which ones to stay away from either.
Many thanks,
Doug Keene Shriners Hospital for Crippled Children Portland, Oregon
-------------------------------------------------- } We have been using the Ilford 2150 RC for over a year, maybe two by now. The results are quite good, however there have been mechanical problems with it from the get go. We had to replace bearings early on and have had to dismantle and re-assemble the rollers over and over to keep it from squeaking and sqealing and grinding. So it has been a mixed bag for us. We do about 10,000 prints a year and it has been a real time-saver too -at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at- Greg Erdos Phone: 352-392-1295 Scientific Director, ICBR Electron Microscopy Core Lab 218 Carr Hall Fax: 352-846-0251 University of Florida E-mail: gwe-at-biotech.ufl.edu Gainesville, FL 32611 -at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
The best processor out there is the Mohrpro. We have been using one for about 8 years and recommend it when ever we can. It is easy to use and maintain, 2 min. dry to dry, permanent (not stabilized) and uses RC paper and Kodak Fixer. We do purchase the developer from Mohr. The current cost for paper up to 8" width is $4295, the Mohrpro8. and 14" width $4800, the Mohrpro14. (They do sell rebuilt machines as well). They stand behind their product and I can only say great things about Mohr. For information contact: Bob or Jim Jackson (tell them Linda at Loyola sent you!) Mohr Enterprises 65 E. Palatine Rd. Suite 103 Prospect Heights, Il 60070 1-847-465-0048
Linda Fox Loyola University Medical School lfox1-at-wpo.it.luc.edu
Dear Doug: We've had our Mohr Pro8 for about 9 months and love it. This model came highly recommended by other users in the Boston area who had used them much longer than us. We now wonder how we put up with the tray method for so long! If you want more info, E-mail or call me. Don Gantz Boston Univ School of Medicine gantz-at-med-biophd.bu.edu 617-638-4017
I have used an Agfa system, chemistry and paper for 12 years. The processor was not sufficiently robust nor reliable for us. In any case it is no longer available. The Agfa Variable Contrast Premium paper is my favorite emulsion for scientific work. I recommend that you consider the Durst Printo system. It is modular so you can build it to suit your own needs. It is my choice of currently available systems.
: "Larry D. Ackerman" {mishot-at-itsa.ucsf.edu} -------------------------------------------------------------------
At 10:12 AM 3/7/96 -0600, you wrote: } The best processor out there is the Mohrpro. We have been using one } for about 8 years and recommend it when ever we can. It is easy to } use and maintain, 2 min. dry to dry, permanent (not stabilized) and } uses RC paper and Kodak Fixer.
I must respectfully disagree. I was personally responsible for implementing both an Ilford 2150 and a Mohr Pro 8" Model in a multiuser lab. While I liked the Mohr and our users were mostly happy with it, from an ease of use and maint. point of view, I would have to go with the Ilford.
The 2150 ran flawlessly for several years, then needed minor (in-house) maintenance - float sensors went bad. It will process up to 20" wide sheet, in 90 secs. dry-dry, and the rollers stay submerged in the chemistry, which seems to keep them cleaner (no dried on chemicals to deal with).
The Mohr has some rollers partially exposed, which often was a source of roller marks, and were more difficult to clean. We used it primarily for negatives (TEM & SEM). It performed well, just not as smoothly as the Ilford.
Overall, I had very good support from both Ilford and Mohr (though neither produced problems that really tested the waters). I would recommend both, but for different apps - Ilford for RC Prints (does not do film), and the Mohr for negs. With the caveat that the Mohr will require more hands-on time.
JCL ========================================================= James C. Long Manager/Materials Analysis Lab Electrosource, Inc. 512-445-6606 jlong-at-electrosource.com
Scott D. Whittaker 218 Carr Hall Research Assistant Gainesville, FL 32610 University Of Florida ph 904-392-1295 ICBR EM Core Lab fax 904-846-0251 sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/
Dear Sir: We have a dissassembled Siemens ELMISKOP 102 TEM which is available free of charge for the taking. It was purchased in 1972, and was working just fine when we moved it. We have a newer scope that we like better. Please respond if you are interested. G. C. Wisler USDA-ARS Salinas, Ca 93905
Used TEM available: 1973 Siemens ELMISKOP 102. Was in fine working condition when it was retired. Has been dissassembled and is in storage. Interested persons contact: Tom Nelson USDA-ARS 1636 E. Alisal St. Salinas, CA 93905
} Just a short question. What about health risks for TEM users (and } sample preparation) when expecting a baby? } Dear Francisca, The MSA Technologists Forum put together a short book published by San Francisco Press titled Electron Microscopy Safety Handbook, VC Barber & J A Mascorro editors. I didn't see any referrence to pregnant workers in the Table of Contents, but the book is very useful and inex- pensive. I ordered my copy in 1994 from
San Francisco Press, Inc. Box 426800 San Francisco CA 94142-6800 USA as Electron Microscopy Safety Handbook, 2nd edition ISBN # 0-911302-72-7
For radiation, the standard for pregnant workers is an order of magnitude lower than for other workers, and I'd apply the same reasoning for chemical hazards, except that I'd be even more cautious for critical periods of pregnancy when specific organ systems are developing. Congra- tulations and good luck. Yours, Bill Tivol
I worked in a project dealt with ultrasection of fish otolith for several years. My purpose was to get thin section in {50nm for EELS analysis. Fish otolith is a piece of rock in fish's inner ear and very hard and fragle. I have been succesfull in getting such ultrasection in Ultracut E machine. Few technological points may help you to get better sections.
Resin's hardeness can not macth the hardeness of otolith no matter what recipes you use. Also the role of resin is for hold the sample instead of embedding. When knife touch sample, the sample get a moment tending to rotate, thus a gap between sample and resin will occur. Also sample can not be cut due to the elastisity of resin but none of sample. After few cutting cycles, the sample may be cut in thick section. My method for these problems is as following:
After a few cutting, take block out of machine, put a drop of Spurr resin on cutting surface (sample originally embedded in Epon resin)to fill out the gap, put it in oven for polymerilization. Repeat above for several times. This will have the hard sample to press its surounding of resin and increse density of resine to have better holding result. Shortly, I grind the block with #1200 sand paper instead of cutting. By this way, I can get silve-gold color section in 0.2-0.5 mm squar of sample area.
Picking the sections is a difficult job. I use carbon film with copper grid to pick the sections up. Sock the carbon film with 10%-30% alcohol then touch the sections. Alcohol reduces water surface tension and protect sections from damage. But only 10-30% chances can get good sections on grid.
Hope this helps
Zhiyu Wang Department of Biosystem Engineering University of Hawaii at Manoa Honolulu Hawaii zhiyu-at-hawaii.edu
jean ross wrote: } } I have been working on a project with a dental grad student involving } ultramicrotomy of undecalcified human teeth. The samples were prepared by } slicing them into approx. 100um slices and then embedding them in Epon. They } section just great but something happens when they're picked up on grids which } causes just the dentin portion of the section to wrinkle. I use formvar coated } 200 mesh copper grids, stabilized with carbon and glow discharged. I spread the } sections out in the boat with chloroform (I have used xylene too) and have } picked them up every differnt way I could think of. Is it my support film? } Would a thicker section be desirable (sections are usually 90-95 nm)? It is } only the dentin part of the section that has this problem, the Epon and } composite resin reconstruction are perfectly flat. Does anybody have any } suggestions? } } Thanks in advance, } } Jean Ross } Central Microscopy Research Facility } University of Iowa } 85 EMRB } Iowa City, IA 52242 } (319) 335-8142 } Web site: http://www.uiowa.edu/~cemrf
jean ross {jeanross-at-emiris.iaf.uiowa.edu} Hi, Jean:
I worked in a project dealt with ultrasection of fish otolith for several years. My purpose was to get thin section in {50nm for EELS analysis. Fish otolith is a piece of rock in fish's inner ear and very hard and fragle. I have been succesfull in getting such ultrasection in Ultracut E machine. Few technological points may help you to get better sections.
Resin's hardeness can not macth the hardeness of otolith no matter what recipes you use. Also the role of resin is for hold the sample instead of embedding. When knife touch sample, the sample get a moment tending to rotate, thus a gap between sample and resin will occur. Also sample can not be cut due to the elastisity of resin but none of sample. After few cutting cycles, the sample may be cut in thick section. My method for these problems is as following:
After a few cutting, take block out of machine, put a drop of Spurr resin on cutting surface (sample originally embedded in Epon resin)to fill out the gap, put it in oven for polymerilization. Repeat above for several times. This will have the hard sample to press its surounding of resin and increse density of resine to have better holding result. Shortly, I grind the block with #1200 sand paper instead of cutting. By this way, I can get silve-gold color section in 0.2-0.5 mm squar of sample area.
Picking the sections is a difficult job. I use carbon film with copper grid to pick the sections up. Sock the carbon film with 10%-30% alcohol then touch the sections. Alcohol reduces water surface tension and protect sections from damage. But only 10-30% chances can get good sections on grid.
Hope this helps
Zhiyu Wang Department of Biosystem Engineering University of Hawaii at Manoa Honolulu Hawaii zhiyu-at-hawaii.edu
jean ross wrote: } } I have been working on a project with a dental grad student involving } ultramicrotomy of undecalcified human teeth. The samples were prepared by } slicing them into approx. 100um slices and then embedding them in Epon. They } section just great but something happens when they're picked up on grids which } causes just the dentin portion of the section to wrinkle. I use formvar coated } 200 mesh copper grids, stabilized with carbon and glow discharged. I spread the } sections out in the boat with chloroform (I have used xylene too) and have } picked them up every differnt way I could think of. Is it my support film? } Would a thicker section be desirable (sections are usually 90-95 nm)? It is } only the dentin part of the section that has this problem, the Epon and } composite resin reconstruction are perfectly flat. Does anybody have any } suggestions? } } Thanks in advance, } } Jean Ross } Central Microscopy Research Facility } University of Iowa } 85 EMRB } Iowa City, IA 52242 } (319) 335-8142 } Web site: http://www.uiowa.edu/~cemrf
Message-ID: {3151A34A.25E8-at-hawaii.edu} jean ross {jeanross-at-emiris.iaf.uiowa.edu} CC: Microscopy-at-Sparc5.Microscopy.Com
Hi, Jean:
I worked in a project dealt with ultrasection of fish otolith for several years. My purpose was to get thin section in {50nm for EELS analysis. Fish otolith is a piece of rock in fish's inner ear and very hard and fragle. I have been succesfull in getting such ultrasection in Ultracut E machine. Few technological points may help you to get better sections.
Resin's hardeness can not macth the hardeness of otolith no matter what recipes you use. Also the role of resin is for hold the sample instead of embedding. When knife touch sample, the sample get a moment tending to rotate, thus a gap between sample and resin will occur. Also sample can not be cut due to the elastisity of resin but none of sample. After few cutting cycles, the sample may be cut in thick section. My method for these problems is as following:
After a few cutting, take block out of machine, put a drop of Spurr resin on cutting surface (sample originally embedded in Epon resin)to fill out the gap, put it in oven for polymerilization. Repeat above for several times. This will have the hard sample to press its surounding of resin and increse density of resine to have better holding result. Shortly, I grind the block with #1200 sand paper instead of cutting. By this way, I can get silve-gold color section in 0.2-0.5 mm squar of sample area.
Picking the sections is a difficult job. I use carbon film with copper grid to pick the sections up. Sock the carbon film with 10%-30% alcohol then touch the sections. Alcohol reduces water surface tension and protect sections from damage. But only 10-30% chances can get good sections on grid.
Hope this helps
Zhiyu Wang Department of Biosystem Engineering University of Hawaii at Manoa Honolulu Hawaii zhiyu-at-hawaii.edu
jean ross wrote: } } I have been working on a project with a dental grad student involving } ultramicrotomy of undecalcified human teeth. The samples were prepared by } slicing them into approx. 100um slices and then embedding them in Epon. They } section just great but something happens when they're picked up on grids which } causes just the dentin portion of the section to wrinkle. I use formvar coated } 200 mesh copper grids, stabilized with carbon and glow discharged. I spread the } sections out in the boat with chloroform (I have used xylene too) and have } picked them up every differnt way I could think of. Is it my support film? } Would a thicker section be desirable (sections are usually 90-95 nm)? It is } only the dentin part of the section that has this problem, the Epon and } composite resin reconstruction are perfectly flat. Does anybody have any } suggestions? } } Thanks in advance, } } Jean Ross } Central Microscopy Research Facility } University of Iowa } 85 EMRB } Iowa City, IA 52242 } (319) 335-8142 } Web site: http://www.uiowa.edu/~cemrf
} A colleague and I were discussing microprobe analysis of garnets and } he remarked that their analysis of Almandine often produce high totals, } a phenomenon I have also observed with high Fe garnets. I know that } others have observed this as well but has anyone come up with an } explanation ?
Yes, the microprobe analysis of garnets is problematic. Actually, it is silicate minerals of the Fe-Mg solid solution series in general that share this. Here is a short summary of my understanding of the problem:
1. It is primarily the absorption correction component of the ZAF algorithms that is responsible for over-correcting for x-ray absorption thus leading typically to high totals in the analysis (i.e. for garnets 101-102, and for olivines more like 101% in the total). Here I am assuming that end member oxide standards have been used (SiO2 for Si, MgO for Mg, and Fe2O3 for Fe, for example). So when one uses these standards, the analysis of a garnet or olivine yields a high total. The culprit may be that the mass absorption coefficients are in error for Mg (in particular), but also Si and Fe; these erroneous values lead us to the high totals due to overcorrection using a "faulty" mac. John Donovan at Berkeley drew my attention to this, by the way.
The problem in general is that we cannot simply adjust the mac values to suit our needs in a particular compositional system because we may not observe the same problem in a different system.
I don't remember the particulars, but it is also known that some factors in the fluorescence algorithms were originally fudged to work for metals (stainless steel), and that this optimization does not hold for silicate systems.
So there are problems in several components of typical ZAF correction schemes and the parameters they use. Both ZAF and Phi-rho-z schemes use macs for absorption correction, by the way.
2. One really needs to use a standard as close in composition to the sample to be analysed when dealing with Fe-Mg garnets and olivines. I get good results for olivines using fayalite for Fe and an Fo90 olivine for Mg and Si when analyzing olivines that are in the Fo70-90 range. Of course all Fe can safely be assumed to be Fe2+ in these systems (but Fe3+ in olivines is not unknown).
Garnets are a different story. I still observe high totals when using only garnet standards to analyze garnet samples. This again points to mac problems, but why we have success with olivines but not garnets is an unknown. Garnets are fairly dense and so one wonders about density terms in the equations; however, these terms cancel when the k-ratio is calculated. Really well characterized end-member garnets can be hard to find (like a pure almandine, for example).
I will just mention that even though the garnet stoichiometry seems straightforward, that I have observed fluorine up to several thousand ppm in grossulars, and have been told about hydrogrossular component as well. It is possible, then, that the typical wet chemical analyses of our standard grossulars are incomplete. Fe is present as both Fe2+ and Fe3+, but one must be careful about making charge-balance vs. stoichiometric assumtions to calculate Fe2/Fe3.
3. Calculation of oxygen by stoichiometry works pretty well. You should try analyzing garnets for oxygen sometime (using garnet standards, for example grossular), and you will see that the totals are *really* bad as a result, compared to oxygen by stoichiometry. This points to the problems in analyzing oxygen in general.
4. Note that garnets can exhibit chemical zoning, but that this zoning results in the same average atomic number across this zoning due to coupled substitution, so that backscattered-electron imaging is not as successful at elucidating inhomogeneity as in other systems. This means that the homogeneity of a garnet standard (natural material) is suspect until verified by mapping or linescans.
And that is just what came to mind while I sat here for a few minutes!
Paul Carpenter
+------------------------------------------------------------+ | Paul K. Carpenter | | Division Analytical Facility | | Geological and Planetary Sciences MC 170-25 | | California Institute of Technology | | Pasadena, CA 91125 | | 818-395-6126 (X-ray Lab) 818-568-0935 (FAX, Departmental) | | paulc-at-arms.gps.caltech.edu | +------------------------------------------------------------+
I need to by a computer software or databank which can deal with the some problems we materials scientists usually encounter such as the mass diffusion, thermal expansion and thermal conductivity. Please let me know if anybody has such a software or knows something about that. I would appreciate any clue or information to find those software.
Bingqiang Lei
----------------------------------------------------- Name: Bingqiang Lei Department: Materials and Manufacturing Engineering University: Lulea University of Technology Tel: (46) 920 91233 fax: (46) 920 99309 Email: Bingqiang.Lei-at-mb.luth.se -----------------------------------------------------
} } Thanks to everyone who responded to my call for help. In case anyone is } } interested, the replies split about 70/30 in favor of Eppendorfs, but } } several people said they'd never been able to get them to work for LRW. } [snip] } Just wanted to point out that the term "Eppendorf tube" is a bit } like "whiskey", understood instantly, but in fact refering to a wide range } of product. So, "ep tubes" are made by gazillions of companies and } presumably out of, well, dozens, of different kinds of plastics. Might be } worth finding out from someone in the successful camp exactly where they } bought their ep tubes. }
This is a good point. For consistently good results, we use "real" Eppendorf tubes, i.e. made by Eppendorf. We did have problems with slightly cheaper ones once, though I've forgotten exactly what the trouble was.
Rosemary White __ / Department of Ecology _/ \__/ \ and Evolutionary Biology / \ Monash University / Australia \ Clayton, Victoria 3168 \ ____ / phone 61-3-9905 5670 \_/ \_*_/ fax 61-3-9905 5613 __ email rgwhite-at-vaxc.cc.monash.edu.au \/
id sma018161; Thu Mar 21 17:07:28 1996 Received: (from uucp-at-localhost) by alcor.wadsworth.org (8.6.11/8.6.11) id RAA181 79; Thu, 21 Mar 1996 17:07:45 -0500 Received: from alcor.wadsworth.org (alcor.wadsworth.org [199.184.16.17]) by Spar c5.Microscopy.Com (8.6.11/8.6.11) with ESMTP id QAA12373 for {microscopy-at-sparc5. microscopy.com} ; Thu, 21 Mar 1996 16:10:00 -0600 Received: (from daemon-at-localhost) by Sparc5.Microscopy.Com (8.6.11/8.6.11) id QA A12377 for dist-Microscopy; Thu, 21 Mar 1996 16:10:03 -0600 Received: from Sparc5.Microscopy.Com (sparc5.microscopy.com [206.69.208.10]) by ormail.intel.com (8.7.4/8.7.3) with SMTP id WAA14825; Thu, 21 Mar 1996 22:08:48 -0800 (PST) Received: from ormail.intel.com by relay.hf.intel.com with smtp (Smail3.1.28.1 #2) id m0u001p-000qDLC; Thu, 21 Mar 96 22:08 PST
Dear Francisca When our assistant got pregnant I insisted in her leaving all work involving hazardous chemicals to other people (mainly me). The operation of modern EMs should not be hazardous. Basically, the pregnant operator should feel confident that she and her baby are not at exposed to additional hazards. The risks of something going wrong and the TEM getting the blame (rightly or wrongly) are not worth taking.
Yours sincerely
Dr Stephan Helfer, SSO Mycologist / Plant Pathologist
Royal Botanic Garden Edinburgh, Inverleith Row, EDINBURGH EH3 5LR, Scotland UK
http://www.rbge.org.uk
phone: +44 (0)131 552 7171 ext 280 or +44 (0)131 459 0446-280 (direct digital VoiceMail line) fax: +44 (0)131 552 0382 ============================ 1896 * BRITISH * MYCOLOGICAL * SOCIETY 1996 A century of fungal science ============================
We have an S-2460N without a cold stage and have gotten great results at 25Pa and 18Kv with about 60uA of emission. The cold stage will make a diference but maybe this will give you a place to start.
Jean Ross Central Microscopy Research Facility Univ. of Iowa (319) 335-8142 web site: http://www.uiowa.edu/~cemrf
On Thu, 21 Mar 1996, HENRY P ADAMS wrote:
} We recently acquired a variable pressure sem (Hitachi S3200N) with a Fullam } cold-stage, a researcher wants to image fresh leaves and is particularily } interested in the status (open vs closed) of the stomates. Does anyone } have any suggestions as to temp, pressure, Kv, beam current, etc? } } TIA, } Hank Adam }
Responding to message {Pine.SUN.3.91.960321120104.6864B-100000-at-verdi} from HENRY P ADAMS {hadams-at-nmsu.edu} : } } We recently acquired a variable pressure sem (Hitachi S3200N) with a } Fullam } cold-stage, a researcher wants to image fresh leaves and is particularily } } interested in the status (open vs closed) of the stomates. Does anyone } have any suggestions as to temp, pressure, Kv, beam current, etc? } } TIA, } Hank Adam } On our Electroscan environmental SEM we look at plant tissue at a few degrees above freezing. This allows us to keep them hydrated at a pressure of 5-6 torr water vapor in the chamber. At 0 Celcius the saturated vapor pressure of water is about 4 torr, so at pressures lower than this the material dehydrates very quickly. We typically use fairly low kV (5-10kV) to see more surface detail. Under these conditions the plant material can survive for reasonably long periods of time.
Stuart McKernan University of Minnesota Characterization Facility
I would like to ask if anyone has the goniometer tool kit for a Philips 400 series TEM that they would be willing to sell. I would prefer to hear from someone in the US, but all offers will be considered.
F. Scott Miller Electron Microscope Lab smiller-at-umr.edu University of Missouri-Rolla voice: 314 341 4727 Rolla, MO 65401 USA fax: 314 341 6934
} Greetings, } Tamara wrote: } } } Thanks to everyone who responded to my call for help. In case anyone is } } interested, the replies split about 70/30 in favor of Eppendorfs, but } } several people said they'd never been able to get them to work for LRW. } [snip] } Just wanted to point out that the term "Eppendorf tube" is a bit } like "whiskey", .........
What I should have said was polypropylene Eppendorf-type tubes work fine. Fisher Scientific and USA Scientific make the ones we use. We use doggie toenail clippers to snip off the end of the tube and then tease or cut out the polymerized sample and remount it for trimming and sectioning. If the whole embedding process takes place in the tube, take care to see that there is good exchange of fluids at the bottom of the tube or else you could have trouble.
I have never drunk any whiskey from them however.
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at- Greg Erdos Phone: 352-392-1295 Scientific Director, ICBR Electron Microscopy Core Lab 218 Carr Hall Fax: 352-846-0251 University of Florida E-mail: gwe-at-biotech.ufl.edu Gainesville, FL 32611 -at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Francisca: The lab I work in now rents time on someone else's scope, so I haven't asked about extra saftey precautions they take, but I used to work in a lab with it's own TEM. Our scope was checked yearly by environmental health and saftey to make sure that the sheilding the scope comes with was still doing it's job. It always was, but when we had a pregnant woman using the lab., she wore a lead apron whenever she had to be in the scope room. She also was carefull to wear gloves when handeling chemicals, and use a fume hood. She ended up with a very healthy, rather big, (8 pounds, plus) baby boy. She may have taken more precaution than she needed to but it was better than not being careful enough. Good luck. Karen Pawlowski
On Thu, 21 Mar 1996, Francisca Peiro wrote:
} Hi all, } Just a short question. What about health risks for TEM users (and } sample preparation) when expecting a baby? } } Thank you for your suggestions. } } F. Peiro } ===================================================================== } Enginyeria i Materials Electronics Tel. (34-3) 402-11-39 } (34-3) 402-11-47 } Dep. Fisica Aplicada i Electronica FAX. (34-3) 402-11-48 } Universitat de Barcelona e-mail:paqui-at-iris1.fae.ub.es } Avg. Diagonal 645-647 } 08028 Barcelona, Catalunya } Spain } ===================================================================== } }
} } Just a short question. What about health risks for TEM users (and } } sample preparation) when expecting a baby? } }
[snip]
} Is the same true for SEM and FESEM (field emission SEM)? } - JESS MUNOZ
Dear Jess, The chemical hazards pertain to the preparation methods and are independent of the type of instrument (except as the prep methods are mo- dified for a particular instrument). The radiation hazard arises from brehmsstrahlung x-rays generated by the interaction of the stray beam electrons with apertures, the lens column and anything else that electrons can strike. The amount of potential hazard is dependent on both voltage and current, but the standards for radiation exposure are the same regard- less of instrument type. As another contributor to this thread said, mod- ern EMs are well designed for minimizing exposure and are pretty safe. If there is any concern, a personnel monitoring device, such as a film badge or thermoluminescent detector can be placed at the scope for a month, and the radiation exposure can be monitored, and/or a survey meter can be used to get a real-time readout of the radiation field. Yours, Bill Tivol
=============================================================================== John J. Donovan (510) 642-5459 (phone) Room 301, McCone Hall (510) 643-9980 (FAX) Department of Geology and Geophysics jdonovan-at-seismo.berkeley.edu University of California jdonovan-at-garnet.berkeley.edu Berkeley, CA 94720-4767 ===============================================================================
I recently helped some plant biologists obtain images of pieces of maize leaves with the stomata partially open. We used our ElectroScan ESEM with ElectroScan's Peltier stage (a cooling stage). The images were taken at 5kV with the stage temperature set to about 5.5 degrees C. We increased the chamber pressure to condense water on the leaf (to about 6 Torr as I recall), then reduced the chamber pressure to slowly evaporate the water enough to see the leaf and capture an image. The one image I saved for my image gallery was taken at 5.3Torr. You can see it at our web site:
http://ucmp1.Berkeley.EDU/esem/gallery.htm
I have done similar condensation/evaporation experiments on other subjects using different stage temperatures and chamber pressures, mostly around 5C and 5Torr. The size of the subject and how closely it conforms to the stub surface (we have cup-shaped as well as flat stubs for the Peltier) all make a difference. The above settings will give you a starting point for experimenting to determine what works best for your setup.
Karen Wetmore
} We recently acquired a variable pressure sem (Hitachi S3200N) with a Fullam } cold-stage, a researcher wants to image fresh leaves and is particularily } interested in the status (open vs closed) of the stomates. Does anyone } have any suggestions as to temp, pressure, Kv, beam current, etc? } } TIA, } Hank Adam
***************************************************************** Karen L. Wetmore, Ph.D. Museum Scientist Museum of Paleontology 1101 VLSB #4780 (510) 642-0203 University of California fax (510) 642-1822 Berkeley, CA 94720-4780 karenw-at-ucmp1.berkeley.edu *****************************************************************
} A colleague and I were discussing microprobe analysis of garnets and } he remarked that their analysis of Almandine often produce high totals, } a phenomenon I have also observed with high Fe garnets. I know that } others have observed this as well but has anyone come up with an } explanation ?
The problems with high totals in the Si-Mg-Fe system seem to be caused by a bad mass absorption coefficient utilized by most programs of Mg Ka absorbed by Fe. Most software I have seen use a value tabulated (but not measured) by Heinrich. This use of Heinrich's tabulation is apparently part of the the cause of high totals (when extrapolating from pure oxide or end-member compositions) for many minerals, especially those with high Mg-Fe concentrations, such olivine and garnets.
Please note the following values (soft x-ray) quoted from Heinrich and Henke :
Heinrich Henke (1982)
Mg Ka in Si 802 859 Mg Ka in Fe 6121 5250 Si Ka in Mg 2825 2902 Si Ka in Fe 2502 2305
As you can see there is about 20% difference in the mass absorption coefficients for Mg ka in Fe, although the others are reasonably close. This difference will have a significant effect on the quantitative analysis (about 1 % or so).
I have integrated the Henke value into my software as a default table and used pure end-member olivines as standards for garnet analyses and have not seen problems with high totals. There may be more going on here than just a bad MAC or two, but since this is the largest correction we make to our data, it's worth looking at first.
john
=============================================================================== John J. Donovan (510) 642-5459 (phone) Room 301, McCone Hall (510) 643-9980 (FAX) Department of Geology and Geophysics jdonovan-at-seismo.berkeley.edu University of California jdonovan-at-garnet.berkeley.edu Berkeley, CA 94720-4767 ===============================================================================
} We recently acquired a variable pressure sem (Hitachi S3200N) with a Fullam } cold-stage, a researcher wants to image fresh leaves and is particularily } interested in the status (open vs closed) of the stomates. Does anyone } have any suggestions as to temp, pressure, Kv, beam current, etc? } } TIA, } Hank Adam } We use a Hitachi 2250N, also with a Fullam cold-stage for the moment. . We set to around -15-20 degrees if possible to allow for a temperature gradient through the leaf, and work usually at around 0.1-0.3 torr, adjusting beam current to the minimum convenient. However we generally fast-freeze the leaves in liquid nitrogen before transferring to the SEM stage, to try to minimize any change in stomatal state during the transition. An alternative is to set the frozen leaf on a block of metal (preferably steel) cooled in LN2 - this allows 30 minutes or more of observation before drying artifacts set in.
Sally Stowe
---------------------------------------------------------------------- Sally Stowe |Email: stowe-at-rsbs.anu.edu.au Facility Coordinator |Post: ANU Electron Microscopy Unit |ANUEMU (RSBS) Ph 61 6 249 2743 |Australian National Univ. FAX 61 6 249 4891 |Canberra, http://online.anu.edu.au/EMU/home.htm |AUSTRALIA 0200
Am posting this for a someone who doos not have email at the moment.
They have a fully functioning Hitachi H-600 TEM with scanning attachment available at a reasonable price. Also have available a ultramicrotome, knifemaker, plus.
If interested can either email back to me at cal-at-ssnet.com or call them directly at M.H. Systems at 419-647-6400.
-------------------------------------------------------------- Dr. Larry Stoter Technesis 17, Rocks Park Road, Uckfield, E. Sussex, TN22 2AT, United Kingdom Larry-at-teknesis.demon.co.uk --------------------------------------------------------------
Individuals working in the UK are generally unionized and are satisfied with what unions do for them.
In the US, most are not unionized although some would like to be or at least see points in favor of them. Some have had the option although most have declined it. It appears that in the US you can choose whether to be in the union or not on an individual basis.
In Canada, most are unionized although the attitude toward the union ranges from abivalent through sceptical and resigned to decidedly anti.
Hi, Further to the following, wouldn't a low-tech solution work for identifying the contaminant/s? It should be quite easy to tell if it's sodium (solid, reacts with water to give sodium hydroxide) or mercury (dense metallic liquid). By mixing the oil with water and shaking for a while some of the putative sodium would react, and you could assay the aquatic phase for NaOH. By mixing the oil with a thinning solvent, or just by centrifuging, you'd be able to separate mercury. If neither of these yield positive results you're left with the third option (oil). Speak to a local inorganic chemist, or get a book out of the library on small-scale inorganic qualitative analysis to check out the feasibility of the above.
Ray
} I would filter the sample trough a membrane filter (Millipore?) and wash } with a suitable solvent (n=hexane?) to eliminate the oil. Then dry and } carbom coat. } The residue in the membrane should be easily observed in SEM. } Dagoberto Rodriguez } ------------------------- } } } I have an Amray 1600 Turbo SEM, I have a question about a sample } } } } that I would like to observe. The sample is an oil that a plant } } } } uses for a dosing machine. There are visible spots in the oil that we } } } } suspect may be Hg or Na, or the oil may be a synthetic oil, one that was } } } } not supposed to be used in the first place. I was asked if it is possible } } } } to examine a smear of the oil under SEM/EDX and identify the spots in it, } } } } but I'm thinking no because of the wet oil contaminating my sample chamber, } } } } and also the temperature of the electron beam may volatile the mercury. } } } } Is it not possible to observe this oil with my instrument? Are there any } } } } suggestions that I may follow? } } } } } } Mark Darus } } General Electric Co. } } } } Darus-at-cle.dnet.ge.com } } } }
Ray Hicks ________________________________________________________________________ |University of Cambridge |Tel 01223 330149 | |Department of Medicine |Fax 01223 336846 | |Level 5, Addenbrookes Hospital |e-mail rh208-at-cus.cam.ac.uk | |Hills Road Cambridge |Web Page/ facsmac.med.cam.ac.uk | |CB2 |ftp server 131.111.80.78 | |UK | | |_________________________________|_____________________________________|
Here is a digest of answers to my request of titles of books on biology laboratory activities. Some people asked me to send a synthesis of responses. I would like to thank very much all who have contributed to this list. Bye george giocar-at-risc990.bologna.enea.it www.best.com/~funsci (amateur scientist site)
but a good microscopy techniques book is "Electron Microscopy: Principles and Techniques for Biologists" by John J. Bozzola and Lonnie D. Russell, editors. Jones and Bartlett Publishers, Boston. 1992.
-------------------------------------------------------------------- - the followings are answers to the request about "LM: lab manuals, textbooks"
Best books I know of are John Kiernan's _Histological and Histochemical Methods_ and the Biological Stain Commission's _Staining Procedures_ (9th or 10th [or...?] edition). I don't have Kiernan's book to hand, but if he doesn't respond, I can send you the correct info. (Same for _Staining Procedures_.) I heard there was a good EM book with some procedures written by some guy in southern Illinois...
-------------------------------------------------------------- - I know this book:
Dennis E. Ohman, Experiments in gene manipulation, Prentice Hall Inc. 1988
In message {Pine.LNX.3.91.960325134158.3078A-100000-at-limax.paru.cas.cz} Nebesarova LEM Motejl writes: } I would ask somebody for an advice how to measure by simple way the } temperature in a specimen in a domestic microwave oven and if it's better } to remove quickly the animal tissue specimen from the warm fixation } solution after the microwave exposure or to leave it in for long time there. } Many thanks in advance. } Jana Nebesarova } Laboratory of Electron Microscopy } Institute of Parasitology } Ceske Budejovice } Czech Republic
Dear Jana: your questions are very important with respect to achieving reproducible microwave fixation. 1. I recommend a device called a 'Fix-N-Temp' container for simple, rapid, reliable, and inexpensive temperature measurement of specimens fixed in a microwave oven for EM studies .
a. You can make a 'Fix-N-Temp' container by sticking a liquid crystal temperature strip into the bottom half of a 35 mm diameter plastic tissue culture dish (Beckton Dickinson, Lincoln Park, NJ, USA) along the inside diameter of the dish. I recommend using the liquid crystal temperature strip available from Owl Scientific Plastics, Woburn, MA, USA (it measures temperatures between 35 C and 65 C- ideal for the temperature range for microwave fixation). Alternatively, this device can be purchased ready made for several dollars from some EM Supply companies.
b. Place your fixative (up to 5 ml) in the dish, place your tissue (1 mm3 up to 1 cm3) in the fixative and irradiate according to your fixation protocol. You will see the temperature of the solution displayed on the strip as soon as you remove the dish from the microwave oven. The temperature is accurate to within 5 C. The container and liquid crystal strip are reusable.
c. Other methods for measuring temperature require the use of electronic temperature probes which are more expensive, have a slower response time, and are in my experience less accurate. In addition, the temperature probes in microwave ovens are known to distort the microwave fields and cause conductive heating artifacts if placed near a tissue sample.
2. The tissue should be removed from the warmed fixative as soon as microwave irradiation has stopped. Tissues left in warm fixative are damaged by conductive heating from the solution. Specimens up to several cubic mm can be fixed in less than 30 seconds in a microwave oven that has been properly calibrated.
These issues are discussed in detail in the following two references: 1. Login GR, Dvorak AM. Methods of microwave fixation for microscopy. A review of research and clinical applications: 1970-1992. Prog Histochem Cytochem 1994;27/4: 1-127.
2. Login GR, Dvorak AM. The Microwave Toolbook. A Practical Guide for Microscopists (Beth Israel Hospital, Boston, 1994).
Please contact me if you would like additional information.
Gary R. Login, D.M.D., D.M.Sc. Assistant Professor of Oral Pathology Beth Israel Hospital Department of Pathology 330 Brookline Avenue Boston, Massachusetts, 02215
Dear Francisca, I work in the Materials Department at the University of Oxford, and carry out a lot of TEM work, especially using 400kV JEOL machines. I have had two pregnancies whilts using the TEMs, as has another woman in my department, all with absolutely no problems at all. We had the Radiation Protection Office check our machines for radiation levels and they all came way below any danger levels. The only difficulty comes when you get too big to reach the console!!
When I was pregnant I did almost no lab work involving hazardous chemicals (fixing, embedding and photography particulary). I cut sections, but was careful of the resin (no sawing blocks in half). I kept EM time to a minimum. There are regulations in Aust. stipulating what pregnant women can be exposed to and the concentrations are MUCH lower than for general workers, but basically steer clear of the lot if you can. Babies and the adults they grow into are a lot more important than missing out on processing a few specimens - someone else can do it if it's important. If the powers that be don't agree, stand up for your babies rights and don't give in.
Diana van Driel Dept Ophthalmology Sydney University AUSTRALIA 2006
I would like to subscribe: adleman-at-cs.usc.edu (I am unsure that this group is appropriate for my needs so it is possible I will unsubscribe after a short trial) Thanks
The Particle Atlas is an invaluable resource for particle analysts. Volumes 3 and 6 may contain SE micrographs and EDS spectra of use to you. The Particle Atlas was originally printed as a six-volume hardbound edition:
The Particle Atlas. ed. Walter McCrone. Ann Arbor, MI: Ann Arbor Science Publishers, Inc.
The Particle Atlas is also available on CD-ROM from MicroDataware (800/582-6624). See a review by Walter McCrone:
McCrone, W. "The Particle Atlas, Electronic Edition," American Laboratory (April 1993): 39-44.
James Martin Director of Analytical Services and Research Williamstown Art Conservation Center
standard disclaimer of financial interest
On Mon, 25 Mar 1996, Jesus Munoz wrote: n } } Hi all. } } I'm currently doing EDS (Energy Dispersive Spectroscopy, some call it } EDX) analysis of contaminants caught in our production line. As you } know, EDS gives the composition of a substance under analysis. But } what's more important is for us to know/identify what the material is } and where it came from (ex. Fe, Ni, Cr is stainless steel, Powder has } Si & Mg). } } Is there a book, magazine, atlas, or anything that could give me such } information? I'd appreciate any help you could give. Thanks.. } } JESS MUNOZ } }
Mr-Received: by mta RANDD; Relayed; Mon, 25 Mar 1996 14:08:11 -0600 Mr-Received: by mta MCM$RAND; Relayed; Mon, 25 Mar 1996 14:08:13 -0600 Mr-Received: by mta RANDB; Relayed; Mon, 25 Mar 1996 14:08:21 -0600 Alternate-Recipient: prohibited Disclose-Recipients: prohibited Content-Return: prohibited
Jess,
You could try the McCrone Particle Atlas. It is no longer in print but an electronic copy is available on CD-ROM from:
MicroDataWare 2894 Tribune Ave Hayward, CA 94542 Phone: 1-800-582-6624
The atlas contains microscopic data from a variety of materials including optical micrographs, SEM and EDS.
I would ask somebody for an advice how to measure by simple way the temperature in a specimen in a domestic microwave oven and if it's better to remove quickly the animal tissue specimen from the warm fixation solution after the microwave exposure or to leave it in for long time there. Many thanks in advance. Jana Nebesarova Laboratory of Electron Microscopy Institute of Parasitology Ceske Budejovice Czech Republic
Bob: Why not simply try Periodic acid-Schiff? Periodic acid will cleave the glyceryl C-C bonds adjacent to -OH groups; the aldehydes are then demonstrated with Schiff. Sverker
} Does anyone know of a stain that is specific to either carbonyl or hydroxyl } groups? If possible, I would like to find a way to visibly stain gylceryl } monostearate on the surface of polypropylene.
********************************************************* Sverker Enestrom M.D., Ph.D. Department of Pathology University of Linkoping, Sweden Phone: +46 13 22 15 20 Fax: +46 13 13 22 57 *********************************************************
I want to buy a 8 mm diamond knife to cut material embedded in historesin, but I would like some information about it. Does anyone have any experience with this material? Who sells it and how much does it cost? I will be very grateful for any help.
_____________________________________________________________________________ Francisco Javier Hernandez Blazquez * Av. Prof. Lineu Prestes, 1524 Departamento de Histologia e Embriologia * 05508-900 Sao Paulo Instituto de Ciencias Biomedicas * e-mail fjhblazq-at-spider.usp.br Universidade de Sao Paulo * fjhblasq-at-biomed.icb2.usp.br ______________________________________________________________________________
A colleague is needing compositional analysis from 10 nm layers. He is interested in doing focused ion beam analysis considering sample prep difficulties with TEM. Since I am not familiar with FIB, is this a viable technique for his problem? If it is, is there anyone out there willing to analyze his samples?
} } Hi all. } } I'm currently doing EDS (Energy Dispersive Spectroscopy, some call it } EDX) analysis of contaminants caught in our production line. As you } know, EDS gives the composition of a substance under analysis. But } what's more important is for us to know/identify what the material is } and where it came from (ex. Fe, Ni, Cr is stainless steel, Powder has } Si & Mg). } } Is there a book, magazine, atlas, or anything that could give me such } information? I'd appreciate any help you could give. Thanks.. } } JESS MUNOZ } There is an atlas authored by Walter C. McCrone and John G. Delly, titled the The PARTICLE ATLAS, a 4 volume set, however, vol.IV contains edx spectra and SEM of particles. The other volumes deal with other aspects of particle identification such as princibles and techniques, polarizing microscopy, etc. They are all useful. The set we have in our lab is 2nd ed, copyright 1973 by Ann Arbor Science Publishers, Inc., Ann Arbor, Michigan, USA. ISBN # 0-250-40008-1. I don't know if there is a new edition out, Walter McCrone is the father of particle identification. Good luck, Hank Adams }
Alternate-Recipient: prohibited Auto-Forwarded: prohibited Content-Return: allowed Disclose-Recipients: prohibited Conversion: allowed Importance: normal Priority: normal Sensitivity: Normal
Hi all.
I'm currently doing EDS (Energy Dispersive Spectroscopy, some call it EDX) analysis of contaminants caught in our production line. As you know, EDS gives the composition of a substance under analysis. But what's more important is for us to know/identify what the material is and where it came from (ex. Fe, Ni, Cr is stainless steel, Powder has Si & Mg).
Is there a book, magazine, atlas, or anything that could give me such information? I'd appreciate any help you could give. Thanks..
JESS MUNOZ
The following is an attached File item from cc:Mail. It contains information that had to be encoded to ensure successful transmission through various mail systems. To decode the file use the UUDECODE program. --------------------------------- Cut Here --------------------------------- begin 644 RFC822.TXT
Hello, We have been using the Diatome Histo-Diamond knives for several years to cut Historesin and epoxies. These knives are very durable and a real timesaver for extensive serial sectioning and for cutting things that savage glass knives, like a little bone or otoconia. They've survived sectioning up to 5 microns and cut well down to 0.5. At 0.5 microns the sections start to look a little scratchy. They won't section a lot of bone, someone here made an unauthorized attempt to section an untrimmed undecalcified cochlea. The knive sectioned with lots of scratch marks afterwards, but cut well after being sent back for resharpening.
You will want to clean the knive periodically with mild detergent for optimal performance.
Regards,
Glen MacDonald Research Scientist Hearing Research Laboratories of the Virginia Merrill Bloedel Hearing Research Center Box 35-7923 University of Washington Seattle, WA 98195-7923 (206) 616-4156 glenmac-at-u.washington.edu
On Mon, 25 Mar 1996, Francisco J. Hernandez wrote:
} } I want to buy a 8 mm diamond knife to cut material } embedded in historesin, but I would like some } information about it. } Does anyone have any experience with this material? } Who sells it and how much does it cost? } I will be very grateful for any help. } } _____________________________________________________________________________ } Francisco Javier Hernandez Blazquez * Av. Prof. Lineu Prestes, 1524 } Departamento de Histologia e Embriologia * 05508-900 Sao Paulo } Instituto de Ciencias Biomedicas * e-mail fjhblazq-at-spider.usp.br } Universidade de Sao Paulo * fjhblasq-at-biomed.icb2.usp.br } ______________________________________________________________________________ } } }
At 12:28 PM 3/26/96 -0200, you wrote: } Are there IBM compatible PC based programmes able to assist with doing } stereology from TEM and LM micrographs. What are the hardware require- } ments? } } Thank you } Leon Scott } SA } } You will find an archived discussion about the presentation of stereo pairs on the web page listed at the bottom of this message. Look in the "Tips & Tricks" section. There was some discussion about software. I will be more than happy to e-mail the file to you as well if you are unable to access the page. Cheers
Scott D. Whittaker 218 Carr Hall Research Assistant Gainesville, FL 32610 University Of Florida ph 904-392-1295 ICBR EM Core Lab fax 904-846-0251 sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/
I am starting to look at tapeworms in the Trypanorynch group and was wondering if there is a suggestion for a good fix for the preservation of "actin"? I normally use 2.5% glut in cacodylate buffer. Will this be O.K. or is there something better? I greatly appreciate any help.
Send us your name and address and we'll send you a copy of the beautiful "Microscope Book", a catalog with a lot of information. Or use our 800 #, 440-0311.
Hope all your horses are winners.
Ellie Solit The Cambrex Group
On Mon, 25 Mar 1996, Keith Lewis Allison, Jr. wrote:
} Hello! } I have a small horse breeding operation and am just beginning to use } artificial insemination. I am in need of a microscope for semen } evaluation. I would appreciate any advice you may be able to provide on } the purchase, i.e. objective size, source, options, etc. Please keep in } mind that I am ignorant of microscopy terminology. I do not want } anything exceptionally fancy, but must be functional....and "tough" for } farm use. Thanks in advance for your response! } Keith } }
To: Microscopy ListSer {Microscopy-at-MSA.Microscopy.Com} *** Reply to note of 03/26/96 11:50
The sputter targets I've seen are thin, and when you see them starting to come apart, it's time to change them, if you're concerned about minor contaminants from the base material (I think ours is Al).
Try Ernest Fullam, SPI, etc for replacement targets.
{ } { { } } ===========} Dave King { { { } } } ------------------------------------------------------ { { { {
Hello out there, Local microbiologists want to look at soil samples and the resident microboal flora. My EM lab has all the usual SEM and TEM methods on tap and are doing a library search but if anyone out there has personal experience of
A: looking for (or at) microbes in soil (ON soil) with frozen-hydrated specimens or
B: untreated soil in an environmental SEM.
we would be very interested to learn of your experiences. Thanks in advance,
I am looking for a section counter accessory for the Reichert Ultra Cut E. Anybody out there have one that is not being used?
Doug Davis Staff Research Associate Electron Microscope Facility University of California Berkeley, CA 94720 (510) 642-2085 dbd1-at-uclink4.berkeley.edu
Can anyone tell me when it is time to purchase a new Au target for our Polaron E5100 Series II sputter coater? Ours seems cracked and peeling in places. Over time it is hard to say if there has been a difference in coating. There has been a difference in the current we use to sputter coat. Also, if anyone knows who carries a line of Polaron parts, that information would be helpful as well. THANKS Linda Fox Loyola University Medical School lfox1-at-wpo.it.luc.edu
} Hello All, } The Scanning 96 Meeting in Monterey, CA on 9-12 April is looking for } students to monitor the sessions, ie. run the slide projectors, adjust } lights and hand out information. The student will have the registration } fee waived, in appreciation of helping. For information, please contact } me, or the Scanning 96 office at FAMS, Inc., PO Box 832, Mahwah, NJ } 07430-0832. E-mail: fams-at-holonet.net } Thank you, } Debe Holmberg } USDA-ARS } 916.752.9021 } 916.752-4604 (fax) }
Recently, I sent a message to you all about a used TEM, to see if there was any interest. I have had some, so here is the real offer.
We have a Philips 201c TEM for sale, it is approximately 20 years old. It is functional, but lack of use has caused the vacuum to be less than optimal. With a little TLC this scope could be an excellent workhorse. The investigators who own the scope would be willing to sell it for parts, but they want it removed from their facility, not just scavenged, as they need to make room for a new scope.
Thanks in advance
Cheri Owen Wayne State University Detroit Neurotrauma Institute Detroit, MI (313)577-4648
} Does anyone out there have any experience with EMPA of hair? Preparation, } mounting, etc? Someone here is interested in looking at heavy metals that } thru metabolism are concentrated in the hair.The levels will be low, so } the MDL will be a question, but I don't know how the hair will stand up to } an electron beam. } } John } } } John Fournelle
I just recently did some dog hair. It seemed to hold up well under a 10kV beam. This was for imaging, so was Au/Pd coated, however. Carbon-coating will be more interesting, but should still work. Since your person wants heavy metals, it might be worthwhile using low kVs and going for M-lines. Try obilgue or cross-sectioned hairs--shaft exteriors don't seem to do so well for x-ray; least I've never gotten anything from them. Phil Oshel
&&& Illigitimi non carborundum &&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&& Philip Oshel Center for Electron Microscopy University of Illinois (217)244-3145 oshel-at-ux1.cso.uiuc.edu
Sorry guys. I must have been absent the day they taught us to read
} At 12:28 PM 3/26/96 -0200, you wrote: } } Are there IBM compatible PC based programmes able to assist with doing } } stereology from TEM and LM micrographs. What are the hardware require- } } ments? } } } } Thank you } } Leon Scott } } SA } } } } } You will find an archived discussion about the presentation of stereo pairs on the web page listed at the bottom of this message. Look in the "Tips & Tricks" section. There was some discussion about software. I will be more than happy to e-mail the file to you as well if you are unable to access the page. } Cheers }
Scott D. Whittaker 218 Carr Hall Research Assistant Gainesville, FL 32610 University Of Florida ph 904-392-1295 ICBR EM Core Lab fax 904-846-0251 sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/
-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --
Linda Fox wrote:
Can anyone tell me when it is time to purchase a new Au target for our } Polaron E5100 Series II sputter coater? Ours seems cracked and peeling in } places. Over time it is hard to say if there has been a difference in } coating. There has been a difference in the current we use to sputter } coat. Also, if anyone knows who carries a line of Polaron parts, that } information would be helpful as well.
It sure does sound like you need a new cathode.
The Polaron line is now being produced by VG Microtech in the UK. The "official" distributor for VG Microtech in the USA is Energy Beam Sciences.
Several other firms, such as SPI Supplies are also manufacturing replacement targets for these units. If your system takes the 57 mm solid disc, the SPI replacement cathodes differ from the original equipment cathodes in that the SPI version is 10 mils thick (instead of 3 or 5 mils). Don't forget that SPI offers a precious metals recycling program, granting a 10% discount when the spent cathode is returned at the time of purchase of the new cathode.
Chuck
====================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President 1-(800)-2424-SPI SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: GVKM07A-at-prodigy.com West Chester, PA 19381-0656 USA Customer Service: SpiSupp-at-aol.com
-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --
The following was posted recently:
No plastic capsule or tube that I know of is air-tight enough for LR White.You may double emded with gelatin and get away with a plastic capsule. Kate Connolly
Standing in our exhibit booth, and listening to how some of our more ingenuous customers use some of our products, I have learned that if one takes the UV transparent SPI embedding (silicone) molds, and over fills them slightly, and then places another (identical) mold on top, the capillary action between the two molds really does seal out oxygen to the point that the resin can be UV cured without worry that oxygen will some how interfere.
Of course, after polymerization, the top mold separates easily and the blocks are removed from the "bottom" mold.
There are a number of advantages to using this approach since it is far more easy to properly align samples in a specific direction with respect to what will eventually become the cutting direction.
More information about the SPI Supplies silicone embedding molds can be found in our electronic catalog on the WWW (see below).
Chuck
====================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President 1-(800)-2424-SPI SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: GVKM07A-at-prodigy.com West Chester, PA 19381-0656 USA Customer Service: SpiSupp-at-aol.com
} Can anyone tell me when it is time to purchase a new Au target for } our Polaron E5100 Series II sputter coater? Ours seems cracked and } peeling in places. Over time it is hard to say if there has been a } difference in coating. There has been a difference in the current we } use to sputter coat. Also, if anyone knows who carries a line of } Polaron parts, that information would be helpful as well. } THANKS
Here we using the same model. Normally there is a area which the target suffer a higher rate of sputtering material loss. That is normally at a area at the edge along the perimeter of the target. It eventually leeds to a groove, exposing the target ring. When that happens we replace ours. We buy the Au or Au/Pd in sheets locally and do it our selves. It is cheaper and saves the time spent waiting for the target to arrive. (If it is not in stock we wait 3-8 weeks!) Note that the sheets can be difficult to cut, especially the Au/Pd
Hope this helps.
Stephan H Coetee Electron Microscope Unit Private Bag 3 Wits 2050 Stephan-at-Gecko.biol.WITS.ac.za
Hi Everyone, I am working on a project with TEM investigations of higher waterplants. Untill now I have only a few experiences with electronmicroscopical analyis. In my lab we tried to fix the waterplants with large gaslacunae in the leaves and only two to three cell layers arround it. We have always the problem that the tissue collapse by evacuating the leaves or embedding them into Epon, Spurr etc., when they are not evacuated. The fixation and contrast of cytoplamsic structures are OK. Has anyone an idea or knows a method to solve the problem of tissue collapsing? Is a there a paper published where this phenomen or a appropriate method has been described for submers waterplants, especially with large gaslacunae?
Thanks for your help!
Dirk Voeste
Dr. D. Voeste Ruhr-Univercity Bochum Comparative Endocrinology Research Section ND-5/31 D-44780 Bochum 0234/7004325 (phone) 0234/7094551 (fax) dirk.voeste-at-rz.ruhr-uni-bochum.de
Linda- The Polaron range of specimen preparation equipment for electron microscopy is manufactured by VG Microtech in the U.K. We (Energy Beam Sciences) are the exclusive representative for the Polaron range in the United States; Soquelec Ltd. are the exclusive representatives in Canada. We provide authorized bench service for current and all older models of Polaron instruments, and carry a wide range of spare parts in inventory, including targets for all models of sputter coaters. The current Polaron range is described at our WWW site (http://www.ebsciences.com/). Please contact me directly if I can be of further assistance. Best regards, Steven E. Slap, Vice-President 75767,640-at-compuserve.com
Hi everybody, A friend of mine would like to acquire an RCA EMT electron microscope if they are still available. If they are please get in touch directly with him. Mr. Tom Bunch e-mail: { tbunch2-at-ix.netcom.com } Regards, John Gabrovsek CCF Cleveland,Ohio
Seeing Linda Fox's post just reminded me of something I found out the other day here. Apparently the cheapest source of gold (99.9% pure) is new 'limited edition' gold coins. Don't ask me why! One guy here had a crown about an inch across which he then rolled to produce a 2 inch target for an industial coater. Saved the company a packet.
Richard Beanland GMMTL Caswell, Towcester, Northants NN12 8EQ.
Subject: Time:9:42 AM OFFICE MEMO freezing Date:3/26/96
I am looking for available information about freezing a tissue(brain) for LM histochemistry and immunocytochemistry. Also, I need information how to estimate the optical density of a histochemically stained sections? Are there books, those could give me such information? Any information would be very much appreciated. Igor_Polyakov-at-qmgate.arc.nasa.gov
We have been using Gatan "G-1" epoxy to prepare cross sections for TEM analysis. Does anyone know what this "G-1" epoxy is? More importantly, is this an epoxy that be purchased at, say a hardware store? Or perhaps some one knows of a substitute that works as well or possibly better that can be purchased locally.
thanks in advance, John
John Phelps NIST Materials Reliability Division 325 Broadway Boulder, CO 80303 ph. 303-497-7570 fax. 303-497-5030
Thanks to all who responded to my request for information on 3/11/96. I have compiled a list of fees for selected services for 12 USA facilities and 4 facilities in other countries. If you would like a copy, let me know if you prefer to receive it by FAX or by e-mail (Mac or IBM format).
Arthur R. Hand Central EM Facility Univ of Connecticut Health Ctr e-mail: hand-at-nso1.uchc.edu
A note related to Richard Beanland's comment about using a flattened gold coin as a sputter target to save money:
I use 1/4 oz Canadian Maple coins (purchased at a local coin dealer) as a source of gold (99.99% pure) in my thermal vacuum evaporation system. I cut them into small pie-shaped pieces using heavy duty wire cutters that have been carefully cleaned and degreased. These pieces can be futher subdivided prior to weighing them and placing them in the evaporator's tungsten boat.
--------------------------------------------------------- Dr. Michael E. Knotts E-mail: ph281mk-at-prism.gatech.edu Georgia Tech / School of Physics / Atlanta, GA 30332-0430 Tel: (404) 894-3422 FAX: (404) 894-9958
Greetings list server friends. A recent request regarding FIB information caught my eye and I forwarded it to a colleague who works in our FIB systems division. His input is below. In an effort to not clog up the list server with marketing or sales junk, the response is mostly an offering of where to go to find further information.
Cheers, Damon
**************************************
Hello,
This indeed sounds like a FIB-related application. I am a Sales Associate at FEI. As you may know, FEI specializes in the development and production of FIB systems and their components. We would be happy to answer any questions you have regarding this technology.
In response to your friend's situation, we have an on-site lab that can analyze any number of different types of samples, so please contact me if you think I can be of assistance.
If you, your colleagues, or any others on the list server have any additional FIB related inquiries, please feel free to e-mail me directly, off line from this list server, at any time.
Brinker B. Gildersleeve Sales Associate FEI Company
bbg-at-feico.com
} Greetings, } } A colleague is needing compositional analysis from 10 nm layers. He is } interested in doing focused ion beam analysis considering sample prep } difficulties with TEM. Since I am not familiar with FIB, is this a viable } technique for his problem? If it is, is there anyone out there willing to } analyze his samples? } } Jeff Shield
FEI Company 7451 N.E. Evergreen Parkway Hillsboro, OR 97124-5830
} We have been using Gatan "G-1" epoxy to prepare cross sections for TEM } analysis. Does anyone know what this "G-1" epoxy is? More importantly, is } this an epoxy that be purchased at, say a hardware store? Or perhaps some } one knows of a substitute that works as well or possibly better that can be } purchased locally.
G-1 Epoxy appears to be the same as EPOXY TECHNOLOGY's 353ND epoxy. This adhesive was originally sold for fiber optic work. Anyway, we at BUEHLER sold it until recently when it's carcinogenic properties became better known to us. However, I believe you can still buy it directly from EPOXY TECHNOLOGY INC. in Billerica, MA (Phone: 1-800-227-2201). Last time I bought some, I got a good sized can (without quantity written on the label) estimated at about 15-20oz. for $35.00. However, there are other EM suppliers who sell it in smaller containers for a somewhat reasonable price.
Good Luck. Scott D. Holt BUEHLER 41 Waukegan Rd. Lake Bluff, IL 60044 (847)295-4546
On the behalf of The International Society of Molecular Morphology
Would like to take the opportunity to announce the
FOURTH INTERNATIONAL CONFERENCE & WORKSHOP on MOLECULAR MORPHOLOGY
June 3-4, 1996 - Conference June 5 -6, 1996 - Workshop in
Montreal, Canada
CONFERENCE FEATURING Advances in Principles, Techniques and Applications in Research and Diagnosis of:
- In Situ PCR - In Situ Hybridization - Immunohistochemistry - Immunogold-Silver Staining - Immunogold Electron Microscopy - Microwave Immunohistochemistry - Atomic Force Probe Microscopy - Confocal Microscopy - Antigen Retrieval - Image Analysis
Call for Abstract Submission DEADLINE: APRIL 15, 1996
The abstract should be typed single space on white paper within 9 x 7 inch (23 x 18 cm) typed space. Total of two pages per abstract. Photographs and references may be included. Please follow the style of CELL VISION, in which the Proceedings will be published. Abstracts should be submitted in duplicate.
THREE HANDS-ON WORKSHOPS (at the laboratories of Dept. of Anatomy, University of Montreal) 1 In Situ PCR and In Situ Hybridization 2 Immunogold EM and Immunogold-Silver Staining 3 Microwave Fixation, Antigen Retrieval and Immunohistochemistry
June 2, 1996 (Sunday), 6.30-9.30 pm: An optional preparation lecture on "Molecular Biology for the Uninitiated"
ORGANIZING COMMITTEE CO-CHAIRMEN Jiang Gu, M.D., Ph.D. Deborah Research Institute Browns Mills, New Jersey, USA
Moise Bendayan, Ph.D. University of Montreal Montreal, Canada
MEMBERS Virginia Anderson, M.D. Health Science Center at Brooklyn State University of New York Brooklyn, New York, USA
Gerhard Hacker, Ph.D. Institute of Pathology General Hospital, University of Salzburg Salzburg, Austria
Lawrence DeBault, Ph.D. Oklahoma University Health Center Oklahoma City, Ok, USA
Shahla Masood, M.D. University of Florida Health Science Center Jackonsville, Florida, USA
Robert Day, Ph.D. University of Montreal Montreal, Canada
David Kersten, M.D. Ealing London, UK
---------------------------------------------------------------------------- ------------------------------------- ADVANCED REGISTRATION FORM (please print and use photocopies for additional forms)
CHOICE OF WORKSHOP (circle one): 1 2 3 ---------------------------------------------------------------------------- ------------------------------------- ADVANCED REGISTRATION FREE (for one of three): - For the two-day conference $250 (US) - For the two-day workshop $350 (US)
Make check payable to CELL VISION. A 15 % discount for members of the "International Society of Molecular Morphology" and students (with proven ID). A 15% discount will be reimbursed upon becoming a member of the society before or at the conference. Please book the hotel room directly by calling The Best Western Hotel in Montreal, Canada (800) 361-3000 or Fax (514) 861-4089. You can obtain a special discounted room rate at $79 (Canadian) per day (rate includes breakfast) by identifying yourself as a participant of the conference/workshop. Student dormitory available at University of Montreal (15-minute subway transporation to conference location, 5 minute walk to workshop location) at $35 (Canadian) per day by calling (514) 343-6531.
Send abstract, registration form, and registration fee to: CELL VISION-JOURNAL OF ANALYTICAL MORPHOLOGY, EDITORIAL OFFICE, DEBORAH RESEARCH INSTITUTE 1 Trenton Road, Browns Mills NJ 08015-1799, USA Phone: (609) 735-0477 Fax: (609) 735-0478
For further information please direct your inquiries by email to: morphology-at-scottscientific.com _______________________________________________ SCOTT SCIENTIFIC P.O. Box 66552 Station Cavendish, Montreal, Quebec, H4W 3J6, Canada
I need a reference to a through TEM/SEM atlas of ultrastructure of prokaryotes and viruses. Or separate atlases for each. Must be 1990 or later. Thanks in advance for any info. Phil
&&& Illigitimi non carborundum &&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&& Philip Oshel Center for Electron Microscopy University of Illinois (217)244-3145 oshel-at-ux1.cso.uiuc.edu
Does anyone out there have any experience with EMPA of hair? Preparation, mounting, etc? Someone here is interested in looking at heavy metals that thru metabolism are concentrated in the hair.The levels will be low, so the MDL will be a question, but I don't know how the hair will stand up to an electron beam.
John
John Fournelle Electron Microprobe Lab Internet:johnf-at-geology.wisc.edu Dept of Geology & Geophysics Office: (608) 262-7964 University of Wisconsin Lab: (608) 265-4798 1215 West Dayton Street Fax: (608) 262-0693 Madison, WI 53706 Amateur radio: WA3BTA/9 http://geology.wisc.edu/~johnf/sx51.html
"The first rule of all intelligent tinkering is to save all the pieces." Aldo Leopold
The G1 is the same material we sell as "Golly G1" (we like to have some fun here!).
The actual material is EpoTek 353ND. It is available from:
Epoxy Technology, Inc. 14 Fortune Drive Billerica, MA 01821
TEL: 800-227-2201 FAX: 508-663-9782
We sell an 8 ounce kit of our Golly G1 for about $50. However, you can buy it directly from Epoxy Technology for between $25-30. An 8 ounce kit will last you a lifetime! I think Gatan sells it iin much smaller containers if that is appealing (However, I don't think it costs any less).
Best regards-
David Henriks TEL: 800-728-2233 (toll-free in USA) South Bay Technology, Inc. 714-492-2600 1120 Via Callejon FAX: 714-492-1499 San Clemente, CA 92673 USA e-mail: 73531.1344-at-compuserve.com sbt-at-msa.microscopy.com
Manufacturers of Precision Sample Preparation Equipment and Supplies for Metallography, Crystallography and Electron Microscopy.
Message-Id: {9603271909.AA7252-at-pho903.sbphrd.com} To: microscopy {microscopy-at-Sparc5.Microscopy.Com} {Beverly_E_Maleeff-at-sbphrd.com}
Philadelphia Society for Microscopy Meeting Notice April 1996
DATE: Wednesday, April 10, 1996
PLACE: Laboratory for the Research of Science and Materials (LRSM) Building, 33rd and Walnut Street, Philadelphia, PA. Parking is available behind the LRSM Building after 5:00 PM.
TIME: 5:30 - 7:30 PM Social hour, hosted by our meeting sponsor. Buffet dinner service and informal seating during this time
7:30 PM Speaker:
Photooxidation of Fluorescent Markers: A Bridge Between Light and Electron Microscopic Cytochemistry
Dr. Giuseppe G. Pietra Professor of Pathology Hospital of the University of Pennsylvania Philadelphia, PA
Abstract: Fluorescent labeling techniques have become very popular in many areas of pathology and cell biology. However, a limitation of fluorescent labeling is the relative low resolution of the optical microscope, even using laser scanning confocal microscopy. Excitation of a fluorescent dye in the presence of 3-3-diaminobenzidine (DAB) oxidizes DAB to an insoluble electron-dense product that can be readily localized by electron microscopy. The application of this method to biological problems will be illustrated in a correlative confocal and electron microscopic study of experimental pulmonary edema. Advantages and limitations of this technique will be discussed.
DINNER: With a Mexican Flair!!
COST: Members $12.00 Student members $6.00 Non-members $15.00
MENU: Margaritas Mexican beer Salsa, chips, pretzels, etc.
Vegetarian chili Taco shells, crispy or soft (matzoh will be available) Sauteed ground beef or chicken Shredded lettuce Grated cheese Diced tomatoes Sour cream Guacamole Refried beans Tortilla chips
Fresh fruit salad
Coffee, decaf or tea
Reservations will be taken by Ms. Pat Overend at the University of Pennsylvania, 215/898-8337. Deadline for reservations will be Friday, April 5. If you have any questions regarding the meeting please feel free to contact Rollin Lakis at 215/898-2013 or lakis-at-sol1.lrsm.upenn.edu. Cancellations must be received by Ms. Overend no later than 5:00 PM, April 5, 1996.
I am interested in determining the best procedure to study tooth buds with the SEM. Any Suggestion!
Dr. David G. Gantt Phone: 1-912-681-5964 Dept. of Biology Fax: 1-912-681-0845 Landrum Box 8042 e-mail: david_gantt-at-gsvms2.cc.gasou.edu Georgia Southern University Statesboro, Georgia 30460-8042
Just a quick note on LR White embedding: We occasionally use flat embedding with LR White into polyethylene (I believe) molds and cover the whole thing with Saran wrap. It is a bit messy but it works. I believe you could overfill the tube sligthly and cover with Saran wrap too. Cheers,
Sarka Lhotak EM Facility, McMaster University Hamilton, Ontario, Canada lhotaks-at-fhs.mcmaster.ca
Several years ago, 1991 I think, a forensic scientist from Scotland Yard was the dinner speaker at Inter-Micro in Chicago. One of the cases he described involved the poisoning of a woman from the Middle East. He detected arsenic along a strand of the woman's hair using SEM-EDS. By correlating the intervals at which arsenic was detected with the growth rate of the woman's hair and her schedule over the preceding months, he demonstrated that she was intentially and repeatedly poisoned in the same city. I recall that this led to an arrest. If you need more info, let me know.
James Martin Williamstown Art Conservation Center Williamstown, MA
scott_l-at-unin1.unorth.ac.za wrote: } } Are there IBM compatible PC based programmes able to assist with doing } stereology from TEM and LM micrographs. What are the hardware require- } ments? } } Thank you } Leon Scott } SAYes. Assuming your images are in TIFF or other common format, the Bioquant software from R&M Biometrics has an excellent stereology toolkit add-on module to the True Color Windows image analysis and topographic morphometry software package. R&M likes to provide the PC integrated with the software and the high-end frame grabber that they use, but the system can be installed on most 486 or Pentium computers able to run Windows. Generally, 16MB RAM and large disk capacity (1 GByte) is recommended for image work. If you wish, I can help you locate the BioQuant dealer in your area. Or, better you can call R&M's office in Nashville, TN at 1-800-221-0549. They will be happy to send you literatue and even to arrange for a demonstration for you.
scott_l-at-unin1.unorth.ac.za wrote: } } Are there IBM compatible PC based programmes able to assist with doing } stereology from TEM and LM micrographs. What are the hardware require- } ments? } } Thank you } Leon Scott } SAYes. Assuming your images are in TIFF or other common format, the Bioquant software from R&M Biometrics has an excellent stereology toolkit add-on module to the True Color Windows image analysis and topographic morphometry software package. R&M likes to provide the PC integrated with the software and the high-end frame grabber that they use, but the system can be installed on most 486 or Pentium computers able to run Windows. Generally, 16MB RAM and large disk capacity (1 GByte) is recommended for image work. If you wish, I can help you locate the BioQuant dealer in your area. Or, better you can call R&M's office in Nashville, TN at 1-800-221-0549. They will be happy to send you literatue and even to arrange for a demonstration for you.
On the behalf of The International Society of Molecular Morphology
Would like to take the opportunity to announce the
FOURTH INTERNATIONAL CONFERENCE & WORKSHOP on MOLECULAR MORPHOLOGY
June 3-4, 1996 - Conference June 5 -6, 1996 - Workshop in
Montreal, Canada
CONFERENCE FEATURING Advances in Principles, Techniques and Applications in Research and Diagnosis of:
- In Situ PCR - In Situ Hybridization - Immunohistochemistry - Immunogold-Silver Staining - Immunogold Electron Microscopy - Microwave Immunohistochemistry - Atomic Force Probe Microscopy - Confocal Microscopy - Antigen Retrieval - Image Analysis
Call for Abstract Submission DEADLINE: APRIL 15, 1996
The abstract should be typed single space on white paper within 9 x 7 inch (23 x 18 cm) typed space. Total of two pages per abstract. Photographs and references may be included. Please follow the style of CELL VISION, in which the Proceedings will be published. Abstracts should be submitted in duplicate.
THREE HANDS-ON WORKSHOPS (at the laboratories of Dept. of Anatomy, University of Montreal) 1 In Situ PCR and In Situ Hybridization 2 Immunogold EM and Immunogold-Silver Staining 3 Microwave Fixation, Antigen Retrieval and Immunohistochemistry
June 2, 1996 (Sunday), 6.30-9.30 pm: An optional preparation lecture on "Molecular Biology for the Uninitiated"
ORGANIZING COMMITTEE CO-CHAIRMEN Jiang Gu, M.D., Ph.D. Deborah Research Institute Browns Mills, New Jersey, USA
Moise Bendayan, Ph.D. University of Montreal Montreal, Canada
MEMBERS Virginia Anderson, M.D. Health Science Center at Brooklyn State University of New York Brooklyn, New York, USA
Gerhard Hacker, Ph.D. Institute of Pathology General Hospital, University of Salzburg Salzburg, Austria
Lawrence DeBault, Ph.D. Oklahoma University Health Center Oklahoma City, Ok, USA
Shahla Masood, M.D. University of Florida Health Science Center Jackonsville, Florida, USA
Robert Day, Ph.D. University of Montreal Montreal, Canada
David Kersten, M.D. Ealing London, UK
---------------------------------------------------------------------------- ------------------------------------- ADVANCED REGISTRATION FORM (please print and use photocopies for additional forms)
CHOICE OF WORKSHOP (circle one): 1 2 3 ---------------------------------------------------------------------------- ------------------------------------- ADVANCED REGISTRATION FREE (for one of three): - For the two-day conference $250 (US) - For the two-day workshop $350 (US)
Make check payable to CELL VISION. A 15 % discount for members of the "International Society of Molecular Morphology" and students (with proven ID). A 15% discount will be reimbursed upon becoming a member of the society before or at the conference. Please book the hotel room directly by calling The Best Western Hotel in Montreal, Canada (800) 361-3000 or Fax (514) 861-4089. You can obtain a special discounted room rate at $79 (Canadian) per day (rate includes breakfast) by identifying yourself as a participant of the conference/workshop. Student dormitory available at University of Montreal (15-minute subway transporation to conference location, 5 minute walk to workshop location) at $35 (Canadian) per day by calling (514) 343-6531.
Send abstract, registration form, and registration fee to: CELL VISION-JOURNAL OF ANALYTICAL MORPHOLOGY, EDITORIAL OFFICE, DEBORAH RESEARCH INSTITUTE 1 Trenton Road, Browns Mills NJ 08015-1799, USA Phone: (609) 735-0477 Fax: (609) 735-0478
For further information please direct your inquiries by email to: morphology-at-scottscientific.com _______________________________________________ SCOTT SCIENTIFIC P.O. Box 66552 Station Cavendish, Montreal, Quebec, H4W 3J6, Canada
On the behalf of The International Society of Molecular Morphology
Would like to take the opportunity to announce the
FOURTH INTERNATIONAL CONFERENCE & WORKSHOP on MOLECULAR MORPHOLOGY
June 3-4, 1996 - Conference June 5 -6, 1996 - Workshop in
Montreal, Canada
CONFERENCE FEATURING Advances in Principles, Techniques and Applications in Research and Diagnosis of:
- In Situ PCR - In Situ Hybridization - Immunohistochemistry - Immunogold-Silver Staining - Immunogold Electron Microscopy - Microwave Immunohistochemistry - Atomic Force Probe Microscopy - Confocal Microscopy - Antigen Retrieval - Image Analysis
Call for Abstract Submission DEADLINE: APRIL 15, 1996
The abstract should be typed single space on white paper within 9 x 7 inch (23 x 18 cm) typed space. Total of two pages per abstract. Photographs and references may be included. Please follow the style of CELL VISION, in which the Proceedings will be published. Abstracts should be submitted in duplicate.
THREE HANDS-ON WORKSHOPS (at the laboratories of Dept. of Anatomy, University of Montreal) 1 In Situ PCR and In Situ Hybridization 2 Immunogold EM and Immunogold-Silver Staining 3 Microwave Fixation, Antigen Retrieval and Immunohistochemistry
June 2, 1996 (Sunday), 6.30-9.30 pm: An optional preparation lecture on "Molecular Biology for the Uninitiated"
ORGANIZING COMMITTEE CO-CHAIRMEN Jiang Gu, M.D., Ph.D. Deborah Research Institute Browns Mills, New Jersey, USA
Moise Bendayan, Ph.D. University of Montreal Montreal, Canada
MEMBERS Virginia Anderson, M.D. Health Science Center at Brooklyn State University of New York Brooklyn, New York, USA
Gerhard Hacker, Ph.D. Institute of Pathology General Hospital, University of Salzburg Salzburg, Austria
Lawrence DeBault, Ph.D. Oklahoma University Health Center Oklahoma City, Ok, USA
Shahla Masood, M.D. University of Florida Health Science Center Jackonsville, Florida, USA
Robert Day, Ph.D. University of Montreal Montreal, Canada
David Kersten, M.D. Ealing London, UK
---------------------------------------------------------------------------- ------------------------------------- ADVANCED REGISTRATION FORM (please print and use photocopies for additional forms)
CHOICE OF WORKSHOP (circle one): 1 2 3 ---------------------------------------------------------------------------- ------------------------------------- ADVANCED REGISTRATION FREE (for one of three): - For the two-day conference $250 (US) -