From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-AUG-1996 00:24:08.19 To: MICROARCHIVE CC: Subj: Re: Stray EM Fields X-Sender: alan.wilson@SoMPop.dsto.defence.gov.au Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Thu, 1 Aug 1996 14:37:05 +1000 To: Microscopy@Sparc5.Microscopy.Com From: alan.wilson@dsto.defence.gov.au (Alan Wilson) Subject: Re: Stray EM Fields High Frequency Earth Loops. We also had an interesting stray fields problem which showed up in high noise counts in X-ray spectra - the amount depending on the scan rate in the SEM! This was traced to a high frequency earth loop (no DC connection) with capacitive coupling associated with the thin plastic shin isolating the X-ray detector from the column. This was solved by passing a few loops of the whole cable going to the X-ray detector through a large toroid to increase the high frequency impedance of this part of the loop. Alan Wilson alan.wilson@dsto.defence.gov.au Senior Research Scientist Ship Structures and Materials Division Aeronautical and Maritime Research Laboratory Defence Science and Technology Organization 506 Lorimer St Fishermens Bend 3207 Victoria Australia ph 61 3 9626 7508, fax 61 3 9626 7816 or 61 3 9626 7087 From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-AUG-1996 03:24:52.69 To: MICROARCHIVE CC: Subj: El.diffraction and CCD. Date: Thu, 1 Aug 1996 08:24:06 +0100 (GMT+0100) From: Nebesarova LEM Motejl To: Microscopy@Sparc5.Microscopy.Com cc: lem@paru.cas.cz Subject: El.diffraction and CCD. Message-ID: MIME-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Many best regards from the Czech Republic! We are Lab. of El. Microscopy, Institute of Parasitology, Czech Academy of Sciences. We also offer EM services to other Institutes and Universities in the Czech in the field of biology. We want also to use electron diffraction on the TEM Philips 420 and to connect CCD or TV camera to microscop in the near future. And there are some problems to solve. Please, I have a few questions. 1. Is there some difference in el. diffraction images obtained by different way? I mean: a/ Normal image is loaded by camera to PC and the diffraction is obtained by computer. b/ El.diffraction image is loaded on the planfilm and this one is processed by laser diffraction. c/ El. diffraction is loaded directly by camera and processed by computer. d/ El. diffraction is loaded on the planfilm and this one is scanned to computer and processed by computer. 2. Exist some camera /CCD or TV/, by which it is possible to load el. diffraction images directly? / I'am afraid that very intensive main beam can destroy the detector/ Thank You Very Much for responses. Milos Motejl Lab. of Electron Microscopy, Institute of Parasitology, Czech Academy of Sciences Branisovska 31 370 05 Ceske Budejovice CZECH REPUBLIC FAX : 042/038/47743 E-mail: lem@paru.cas.cz ............................................................................. From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-AUG-1996 07:27:36.90 To: MICROARCHIVE CC: Subj: Re: TEM/LM flat bottom "beem" caps Date: Thu, 1 Aug 1996 06:39:48 -0500 From: ebs@ebsciences.com Message-Id: <199608011139.GAA04088@Sparc5.Microscopy.Com> X-Sender: ebs@ebsciences.com X-Mailer: Windows Eudora Light Version 1.5.2 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" To: baskin@biosci.mbp.missouri.edu (Tobias Baskin) Subject: Re: TEM/LM flat bottom "beem" caps Cc: microscopy@Sparc5.Microscopy.Com Dear Tobias, Energy Beam Sciences carries the TAAB polypropylene embedding capsules. The advantage of polypropylene is that these capsules can be autoclaved and used at temperatures up to 100 degrees C. They come in in two diameters (6mm and 8mm) and two styles (flat ends and truncated pyramids). They can be found on p45 of our Catalog 3, or, on-line, at http://www.ebsciences.com, in the TEM supplies section of the catalog. Best regards Sonja L. White, Sales & Marketing Secretary ******************************** Energy Beam Sciences, Inc. Adding Brilliance To Your Vision ebs@ebsciences.com http://www.ebsciences.com/ ******************************** From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-AUG-1996 08:24:41.36 To: MICROARCHIVE CC: Subj: Re: TEM/LM flat bottom "beem" caps From: kna101@utdallas.edu Date: Thu, 1 Aug 1996 07:41:09 -0500 (CDT) To: Tobias Baskin cc: microscopy@Sparc5.Microscopy.Com Subject: Re: TEM/LM flat bottom "beem" caps In-Reply-To: Message-ID: MIME-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Hello, By some odd coincidence, I was just trying to find out if such a thing exsited yesterday. Right now, I'm trying to make a beem capsule work by setting it on it's cap and snipping the tip to allow filling from what is now the top. I would love to hear if these flat bottom capsules are available in the US too, so please post your replies to the list. Thankyou, Karen On Wed, 31 Jul 1996, Tobias Baskin wrote: > Greetings, > We have been using some capsules that are like "beem" capsules, > 8mm diameter and with a snap-on cap, only instead of having a cone or a > pyramid, they are simply flat on the bottom. They are made from > polyethylene (polypropylene ones are made too). These are great for flat > embedding. We got ours from TAAB in England, but we have used them up > nearly. Does anyone know if these are for sale in the USA? A quick perusal > of catalogs from several major USA supply houses failed to find 'em. Thanks > for any leads. > Tobias Baskin > > - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - > ___ ____ ^ ____ _____ Tobias I. Baskin > / \ / / \ / \ / University of Missouri > / | / / \ / / Biological Sciences > /___ / /__ /_____\ / /__ 109 Tucker Hall > / / / \ ( / Columbia, MO 65211 USA > / / / \ \ / voice: 573-882-0173 > / /____ / \ \____/ /_____ fax: 573-882-0123 > > > From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-AUG-1996 11:25:28.67 To: MICROARCHIVE CC: Subj: SEM - Problem with M6A Pirani Gauge Controller Message-Id: <2.2.32.19960801155035.006c12e8@192.0.2.2> X-Sender: ez1@192.0.2.2 X-Mailer: Windows Eudora Pro Version 2.2 (32) Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Thu, 01 Aug 1996 08:50:35 -0700 To: Microscopy@Sparc5.Microscopy.Com From: Eugene Zarakhovsky Subject: SEM - Problem with M6A Pirani Gauge Controller I have a rather old Cambridge Stereoscan SEM, and I think the pirani gauge controller may have gone bad. It's an Edwards pirani gauge, model M6A. If anyone knows how to check whether it still works and/or where to find a replacement (Edwards no longer makes that model), please e-mail me. Thank you. -Eugene Zarakhovsky ez1@netcom.com "Show me an angel, I'll paint one." -Courbet From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-AUG-1996 12:29:07.42 To: MICROARCHIVE CC: Subj: Measuring freezing rates Comments: Authenticated sender is From: wesleysm@biology.und.ac.za (James Wesley-Smith) Organization: Biology To: microscopy@Sparc5.Microscopy.Com Date: Thu, 1 Aug 1996 16:38:59 +0200 Subject: Measuring freezing rates Priority: normal X-mailer: Pegasus Mail for Windows (v2.01) Message-ID: <19960801210644974.AAA185@WESLEYSM> I would appreciate to hear from anyone who has had experience in RECORDING freezing rates used in cryofixation. I am particularly interested in establishing the duration and interval of the sampling required. Please reply directly to my address. Thank you. ################################################### James Wesley-Smith Electron Microscope Unit George Campbell Building University of Natal Durban, South Africa http://www.und.ac.za/und/emu/emunit.html ################################################### From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-AUG-1996 13:14:36.65 To: MICROARCHIVE CC: Subj: Re: Stray EM Fields Message-Id: <1.5.4.32.19960801125444.0068d3c0@biotech.ufl.edu> X-Sender: sdw@biotech.ufl.edu X-Mailer: Windows Eudora Light Version 1.5.4 (32) Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Thu, 01 Aug 1996 08:54:44 -0400 To: "Robert Schmitz, Biology" From: Scott Whittaker Subject: Re: Stray EM Fields Cc: microscopy@Sparc5.Microscopy.Com Hi Bob. I will summerize all of the replies in a bit. Meanwhile, there was another thread a while back posted to the server which I have archived. Go to the web page listed at the end of this message. Click on the "Tips & Tricks" button. You will find a link for "SEM Techniques & Instrumentation" which wil point to another link called "Dealing with Drift, SEM". It may prove useful. If you do not have web access, let me know and I will be happy to get the info to you some other way. At 09:44 AM 7/31/96 +0600, you wrote: >>Date: Wed, 31 Jul 1996 08:31:36 -0700 >>From: John Best >>To: sdw@biotech.ufl.edu >>CC: microscopy@Sparc5.Microscopy.Com >>Subject: Re: Stray EM Fields >> >>Scott, >> >>This is an odd image. Typically, with periodic EM or mechanical >>interference, you'll see jagged edges. The amplitude of the "jagginess" >>increases proportionally with magnification. >> >>I assume in this image your referring to the banding on the substrate. Is >>this correct? >> >>My best guess at this time if that the scan rotation was at 90 degrees >>and you've left the SEM's ABC circuit on during image collection. >> >>John Best -- ELMDAS Co. >>jbest@vicon.net > >We don't have Scott's problem with our JEOL 5400 SEM, but we do have this >jagginess problem that John Best deicribes. JEOL has been here and we have >looked for electrical fields. Not a problem. A shield was installed in the >consol to eliminate fields emmanating from the CRT's. No effect The company >service people are stumped, and we are left unable to use the SEM a high mags. >This outlet problem intrigues me, however. Please keep this thread going for a >while. > >Bob Schmitz > rschmitz@uwspmail.uwsp.edu > or > rschmitz@macsrv1.uwsp.edu > (note its macsrv"one" not "el") > Robert (Bob) J. Schmitz > Department of Biology, > University of Wisc. Stevens Point. > Stevens Point, Wisconsin 54481 > ph 715-346-2420 > > ><><><><><><><><><><><><><><><><><><><><><><><><><>< Scott D. Whittaker 218 Carr Hall Research Assistant Gainesville, FL 32610 University Of Florida ph 904-392-1295 ICBR EM Core Lab fax 904-846-0251 sdw@biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/ The home of " Tips & Tricks " From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-AUG-1996 13:25:17.31 To: MICROARCHIVE CC: Subj: Venting Print Processors Message-Id: X-Mailer: Novell GroupWise 4.1 Date: Thu, 01 Aug 1996 10:05:11 -0400 From: Paula Allan-Wojtas To: Microscopy@Sparc5.Microscopy.Com Subject: Venting Print Processors I apologize for bringing this subject up again, but we have recently purchased a print processor and the information I was collecting from the bulletin board has disappeared during my relocation to another province. I was hoping that someone may have a summary of the discussion about the venting of print processors as we are in the midst of renovating our darkroom to install a new one. Please respond to my E-mail address so that I don't clog up the bulletin board with repetitive information. Thanks in advance, Paula. allanwojtasp@em.agr.ca From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-AUG-1996 14:22:35.85 To: MICROARCHIVE CC: Subj: O-Rings Date: Thu, 1 Aug 1996 14:36:31 -0400 (EDT) From: rutledge phil X-Sender: prutle1@umbc8.umbc.edu To: microscopy Subject: O-Rings Message-ID: MIME-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII I was wondering if anybody knows of a supplier of o-rings in the metric size. The suppliers I have dealt with here in Baltimore only have stock of even size o-rings such as 2mm, 3mm, etc. I need a supplier that can provide fractional sizes also such as, 2.3mm, 2.5mm, etc. I am more concerned about the width of the o-ring being fractional than I am of the I.D. or O.D. All of the o-rings I use are in whole numbers as far as the O.D. and I.D. goes. Thanx. O.D.O's Phil From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-AUG-1996 15:25:39.70 To: MICROARCHIVE CC: Subj: Re: suspend mail during vacations Date: Thu, 1 Aug 1996 19:34:10 GMT Message-Id: <199608011934.TAA71068@r05n01.cac.psu.edu> X-Sender: rw9@email.psu.edu Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" To: microscopy@Sparc5.Microscopy.Com From: rw9@psu.edu (Rosemary Walsh) Subject: Re: suspend mail during vacations please unsuscribe Rosemary Walsh #################################################### Rosemary Walsh Electron Microscope Facility for the Life Sciences The Biotechnology Institute for Research and Education 1 South Frear Lab University Park, PA 16802 814-865-0212 email:rw9@psu.edu #################################################### From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-AUG-1996 16:25:44.71 To: MICROARCHIVE CC: Subj: Printer Security Message-ID: Date: 1 Aug 1996 11:10:38 -0800 From: "Murphy, Judy" Subject: Printer Security To: "Microscopy List" X-Mailer: Mail*Link SMTP-MS 3.0.2 I have just purchased a Harris printer which produces black and white photo quality prints on special but affordable paper (i.e.$ 0.68). My concern is that one student could still break the budget. This will be attached over a ether net to our Macs (Mac 8500 and Mac II). We have two printers hooked up to the computers i.e. a Apple 600 dpi laser printer and soon the Harris Printer. I only need the security on the Harris printer. Does anyone know of a password entry to a printer which I might be able to change. Even if I couldn't change it, just having a password entry would help. I have checked with the folks that make MacControl, At East (Apple), and On Guard which are security systems for Macs and they do not have a way of doing that. Any ideas would be appreciated. Thanks in advance, Judy Murphy Judy Murphy, PhD Dept. of Microscopy San Joaquin Delta College 5151 Pacific Ave Stockton, CA 95207 Phone: 209/474-5284 FAX: 209/474-5649 e-mail: murphy@sjdccd.cc.ca.us From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-AUG-1996 16:28:10.22 To: MICROARCHIVE CC: Subj: SET NOMAIL Message-Id: <199608011333.IAA04256@Sparc5.Microscopy.Com> Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Thu, 1 Aug 1996 08:38:10 -0500 To: Microscopy@Sparc5.Microscopy.Com From: zaluzec@Sparc5.Microscopy.Com (Nestor J. Zaluzec) Subject: SET NOMAIL Subscribers.... Please refrain from telling people how to operate the listserver using commands that DONOT exist on this system.. I realize you are trying to give advice but please READ your instructions. That is where you will find out how to do things. You all received instructions on Subscribing and Unsubscribing when you initially subscribed and I periodically (a few times a year repost those instructions to the current subscribers) those are the commands that work no others. Using or telling people to use commands that are NOT IMPLEMENTED do nothing more than increase my work load. Nestor Your Tired & Friendly Neighborhood SysOp who is also the Program Chairman of the Microscopy & Microanalysis 96 Meeting!!! From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-AUG-1996 16:45:52.13 To: MICROARCHIVE CC: Subj: Fall 1996 - TEM Course Announcement Date: Thu, 1 Aug 1996 11:04:16 -0500 Message-Id: <199608011604.LAA04619@Sparc5.Microscopy.Com> To: Microscopy@Sparc5.Microscopy.Com From: becks@sunynassau.edu (Steve Beck) X-Sender: becks@nov1.acs.sunynassau.edu (Unverified) Subject: Fall 1996 - TEM Course Announcement FALL 1996 COURSE ANNOUNCEMENT - Transmission Electron Microscopy (BIO. 221-E2) NASSAU COMMUNITY COLLEGE A fifteen week, fall 1996 semester, course in Biological Transmission Electron Microscopy is being offered by the Biology Department of Nassau Community College. This is a 4 credit course offered ONE EVENING PER WEEK, Thursdays, starting at 5:30pm. Classes will begin on Sept. 5 and end on Dec. 12, 1996. This is a "hands-on" course emphasizing biological specimen preparation, ultra-thin sectioning involving block trimming, glass knifemaking and operation of the ultramicrotomes (Sorvall MT-2B and LKB Ultrotome III), thick and ultra-thin section mounting and contrast staining (UA and Pb citrate), grid support films (formvar, carbon), student operation of the TEM (Hitachi HS-8, Philips EM 300) and production of electron micrographs through the process of black & white photography, and electron micrograph analysis. Students will work on a chosen sample(s) with the goal of producing a portfolio of high quality TEM photomicrographs of that sample(s). The course is widely transferrable and the cost per credit is reasonable at $78 per credit. More information about the Bio-Imaging Center at NCC, course descriptions and syllabi, and the humble beginnings of a student gallery of EM photomicrographs is available at our recently completed web site. The URL is . Any comments or suggestions on the homepage would be appreciated - I'm somewhat new at this! For those without www access, the catalog description is specified below. If you have further questions, you should e-mail me directly at the address below. Interested individuals should register early (prior to Aug. 15) since the course is limited to a total enrollment of ten (10) students. ____________________________________________________________________________ ____ CATALOG DESCRIPTION BIO 221: Transmission Electron Microscopy -- 4 credits Prerequisites: BIO 109-110 or equivalent, CHE 151-152 or equivalent. An introduction to the basic principles of transmission electron microscopy including tissue preparation, microscope (TEM) operation, black & white photography, and micrograph interpretation. The entire laboratory is devoted to the development of skills and preparative techniques involved with the operation of an actual transmission electron microscope. (3 lecture, 3 laboratory hours). Laboratory fee applies. ________________________________________________________________________________ Stephen J. Beck Bio-Imaging Center/Electron Microscopy Department of Biology Nassau Community College Garden City, NY 11530 Voice Mail: (516) 572-7829 Email: URL: From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-AUG-1996 16:52:07.61 To: MICROARCHIVE CC: Subj: SET NO MAIL Is not Implemented!! Message-Id: <199608011327.IAA04242@Sparc5.Microscopy.Com> Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Thu, 1 Aug 1996 08:32:51 -0500 To: Microscopy@Sparc5.Microscopy.Com From: zaluzec@Sparc5.Microscopy.Com (Nestor J. Zaluzec) Subject: SET NO MAIL Is not Implemented!! >Listmembers, >I don't know if this works on every list, but a technique I've used to stop >emailbox overflow when I'm out of town is to use the following command: > >the TO: address should be LISTSERV@MSA.MICROSCOPY.COM >in the text of the message put the line (no signature or footers): >SET MICROSOPY NOMAIL > >When you're ready to get your mail again, send the following message to the >listserv address (LISTSERV@MSA.MICROSCOPY.COM): >SET MICROSCOPY MAIL > >It's probably about as effective as unsubscribing and resubscribing >(provided you remember how to do that without sending your request to all of >us list readers). It does have the advantage of avoiding the welcome >messages that come when you resubscribe (not that we mind Nestor's eloquent >prose...). > >Have a safe vacation. > >Doug >..................................................................... >: Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy : >: Sr. Research Specialist University of Arizona : >: (office: AHSC 4212A) P.O. Box 245044 : >: (voice: 520-626-2824) Tucson, AZ 85724-5044 USA : >: (FAX: 520-626-2097) (email: dcromey@ccit.arizona.edu) : >:...................................................................: > http://www.pharm.arizona.edu/exp_path.html From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-AUG-1996 17:23:24.99 To: MICROARCHIVE CC: Subj: flat bottom caps:summary Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Thu, 1 Aug 1996 14:36:26 -0600 To: Microscopy@Sparc5.Microscopy.Com From: baskin@biosci.mbp.missouri.edu (Tobias Baskin) Subject: flat bottom caps:summary Greetings, I have had many replies to my earlier posting about "flat bottom beem capsules". Thanks one and all. 1) There was a little confusion about "beem" capsules that have the normal cone truncated into a kind of flat pyramid. This is not what I was asking about. Instead, I use capsules with a completely flat bottom, as if you turned the capsule upside down, and used its top for a flat surface (see point two). 2) Several respondents weighed in with methods for do-it-your-self solutions. For example, you can work with a normal "beem" capsule, snap the top on, run a strip of parafilm or wax around the top and turn it upside down. Or, you can cut off the conical bottom and put an extra top on the "bottom", again sealing with wax or parafilm. If just a few samples are needed, these methods are great, and we used to use them. But we process dozens of samples a week (many weeks) and so all of this fabrication is a drag. 3) It was suggested that capsules can be avoided altogether by using flat embedding molds on top of which a second mold is placed, cavity side up, which acts as an air-tight but uv transmittable barrier on top of the samples. 4) Flat bottom polythene capsules, similar if not identical to the ones from TAAB, are now sold by Ted Pella. Energy Beem Sciences apparantly also can get them. EMS and SPI sell vials, perhaps the same as used for holding em grids, that are 8mm in diameter, made of polythene, and apparently used widely for flat embedding. I have no connection with any of these companies other than as a satisfied customer. Hope this helps, Tobias Baskin - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - ___ ____ ^ ____ _____ Tobias I. Baskin / \ / / \ / \ / University of Missouri / | / / \ / / Biological Sciences /___ / /__ /_____\ / /__ 109 Tucker Hall / / / \ ( / Columbia, MO 65211 USA / / / \ \ / voice: 573-882-0173 / /____ / \ \____/ /_____ fax: 573-882-0123 From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-AUG-1996 18:23:01.73 To: MICROARCHIVE CC: Subj: graduate student projects Date: Thu, 1 Aug 1996 15:20:58 -0700 (PDT) From: Jill Craig X-Sender: jcraig@gpu To: Microscopy Newsgroup Subject: graduate student projects Message-ID: MIME-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Hi, I'm looking for some ideas of possible projects at the master's degree level in SEM microscopy. Areas of primary interest would be Materials Science/Engineering, Environmental, and Biological/Medical. I know this is an incredibly diverse area. I'd just like to hear some brainstorming type ideas on things you may have wanted to see investigated, but time/money/etc. restraints don't allow it. I would be happy to either post a summary or keep from posting any suggestions as you prefer. Thanks in advance for your ideas. Jill From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-AUG-1996 19:08:05.50 To: MICROARCHIVE CC: Subj: (Fwd) Posting of TEM Course Announcement From: "Frances Gallery [Neurobiology]" Organization: Neurobiology, SUNY Stony Brook To: Microscopy@Sparc5.Microscopy.Com Date: Thu, 1 Aug 1996 14:37:04 -0500 Subject: (Fwd) Posting of TEM Course Announcement Priority: normal X-Mailer: Pegasus Mail for Windows (v2.23) Message-Id: <1836F732603@neurobio-1.neurobio.sunysb.edu> Dear Aspiring TEMists- 'Just thought I'd share this class info with you, should anyone be interested; I took it last summer and found it a worthwhile intro class! FALL 1996 COURSE ANNOUNCEMENT - Transmission Electron Microscopy (BIO. 221-E2) NASSAU COMMUNITY COLLEGE A fifteen week, fall 1996 semester, course in Biological Transmission Electron Microscopy is being offered by the Biology Department of Nassau Community College. This is a 4 credit course offered ONE EVENING PER WEEK, Thursdays, starting at 5:30pm. Classes will begin on Sept. 5 and end on Dec. 12, 1996. This is a "hands-on" course emphasizing biological specimen preparation, ultra-thin sectioning involving block trimming, glass knifemaking and operation of the ultramicrotomes (Sorvall MT-2B and LKB Ultrotome III), thick and ultra-thin section mounting and contrast staining (UA and Pb citrate), grid support films (formvar, carbon), student operation of the TEM (Hitachi HS-8, Philips EM 300) and production of electron micrographs through the process of black & white photography, and electron micrograph analysis. Students will work on a chosen sample(s) with the goal of producing a portfolio of high quality TEM photomicrographs of that sample(s). The course is widely transferrable and the cost per credit is reasonable at $78 per credit. More information about the Bio-Imaging Center at NCC, course descriptions and syllabi, and the humble beginnings of a student gallery of EM photomicrographs is available at our recently completed web site. The URL is . Any comments or suggestions on the homepage would be appreciated - I'm somewhat new at this! For those without www access, the catalog description is specified below. If you have further questions, you should e-mail me directly at the address below. Interested individuals should register early (prior to Aug. 15) since the course is limited to a total enrollment of ten (10) students. ____________________________________________________________________________ ____ CATALOG DESCRIPTION BIO 221: Transmission Electron Microscopy -- 4 credits Prerequisites: BIO 109-110 or equivalent, CHE 151-152 or equivalent. An introduction to the basic principles of transmission electron microscopy including tissue preparation, microscope (TEM) operation, black & white photography, and micrograph interpretation. The entire laboratory is devoted to the development of skills and preparative techniques involved with the operation of an actual transmission electron microscope. (3 lecture, 3 laboratory hours). Laboratory fee applies. ________________________________________________________________________________ Stephen J. Beck Bio-Imaging Center/Electron Microscopy Department of Biology Nassau Community College Garden City, NY 11530 Voice Mail: (516) 572-7829 Email: URL: From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-AUG-1996 19:22:29.14 To: MICROARCHIVE CC: Subj: Re: "high mag jaggies" (was Stray EM Fields) Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Fri, 2 Aug 1996 09:47:32 +1000 To: Microscopy@Sparc5.Microscopy.Com From: ard@atom.ansto.gov.au (Arthur Day) Subject: Re: "high mag jaggies" (was Stray EM Fields) >Robert and all, > >In my experience, the "high mag jaggies" are most often caused by a >mechanical vibration. Often the roughing pump is the problem. Sometimes >a couple of tennis balls under the roughing pump will decrease the effect >substantially. It's not always this simple though. Also check for cables > touching the vacuum line from the RP. They can transmit these vibes. > >John About a year ago we had an intermittent "high mag jaggies" problem on our 6300. Viewed at TV rate at >50,000x the interference seemed to have a fairly high frequency and underwent rapid variations in amplitude. Some days it was there and other days it wasn't. It was indeed caused by a mechanical vibration -- but in our case it was coming from the stage motors, in particular the Z-axis motor. The effects of this vibration only started to become visible above about 15-20,000x, and it could only just be felt on some days by very lightly touching the motor once you already "knew it had a vibration". Our Jeol engineer was the one who finally figured this out and then he measured a ~2 volt sawtooth ripple on top of the DC holding current to the stage motors. The problem was eliminated by changing some resistors in the (non-Jeol) motor driver hardware, on the recommendation of its vendors, to reduce the DC holding current and hence presumably the absolute magnitude of the ripple that came with it. We have not had any trouble since. Arthur Day, Electron Microscope Unit http: //www.ansto.gov.au/ Ansto Materials Division Phone: 61-2-717-3457 PMB 1, Menai (Sydney), NSW, 2234 Fax: 61-2-543-7179 Australia Email: ard@atom.ansto.gov.au From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-AUG-1996 20:11:32.13 To: MICROARCHIVE CC: Subj: Re: Measuring freezing rates Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Thu, 1 Aug 1996 14:49:18 -0500 To: wesleysm@biology.und.ac.za (James Wesley-Smith) From: tphillips@biosci.mbp.missouri.edu (Tom Phillips) Subject: Re: Measuring freezing rates Cc: Microscopy@Sparc5.Microscopy.Com Back when I was young, I tried measuring the rate of freezing of a thin slice of tissue against a metal mirror surface at liquid nitrogen temp using the quick freeze device now sold commercially as the "gentleman jim". (for a description of the machine but not the freezing rate studies, see Phillips & Boyne, J. Electron Microscopy Techniques 1:9-29 (1984). I built a head for the tissue to rest on that had two electrodes on the bottom surface. the slot in the head on which the tissue was placed was 195 um deep and I used 300 um slides of tissue (liver or electrocytes). the tissue completed the circuit and allowed current from a 6 V source to flow. I added a 5.5 K ohm resister in series and measured a 1-2 V drop across the resister so the tissue was presumably acting as a 6-10 K ohm. when the tissue hit the metal mirror, the signal decreased continuously from the moment of impact.. there was good reproducibility between slices. the total event lasted approx. 2-300 msec with about 30% of the decay in the initial 25 msec. even the initial 25 msec was biphasic with a very flat response and a relatively slower decay. Heuser and Reese used an alternative technique using capacitance. I think this material was included as an appendix in their classic J Cell Biol (1979) 81:280 paper. When I read that paper, I had problems with their assumptions (I am doing this from a 15 year old memory, so I hope I don't have this wrong). They assumed after the first molecular layer had frozen, the tissue could be viewed as 2 dielectrics in series, one water dielectric and one ice. My problem with this is that I viewed the ice as a dielectric and the saline (unfrozen tissue) above as an extension of the copper electrode and therefore I thought the saline was in fact acticing as the upper "plate" of a capacitor. I seem to remember another problem with the maximum breakdown voltage of a dielectric requires a very low applied voltage at the frequencies Heuser et al (and Van Harreveld in earlier studies) had used in their studies and I am not sure their studies took that into account. It has been a long time since I thought about this but if you want more specifics on what I did or read the Heuser paper and have questions about my critique, I will try to figure out my old notes. good luck. Thomas E. Phillips, Ph.D. Associate Professor of Biological Sciences Director, Molecular Cytology Core Facility 3 Tucker Hall University of Missouri Columbia, MO 65211 (314)-882-4712 (voice) (314)-882-0123 (fax) From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-AUG-1996 20:24:02.93 To: MICROARCHIVE CC: Subj: Re: TEM/LM flat embedding with "beem" capsules Mr-Received: by mta SRVR05.MUAS; Relayed; Thu, 01 Aug 1996 10:13:09 -0400 Mr-Received: by mta SRVR05; Relayed; Thu, 01 Aug 1996 10:13:11 -0400 Mr-Received: by mta SRVR01; Relayed; Thu, 01 Aug 1996 10:17:09 -0400 Disclose-Recipients: prohibited Date: Thu, 01 Aug 1996 10:13:09 -0400 (EDT) From: SOBOCIG Subject: Re: TEM/LM flat embedding with "beem" capsules In-Reply-To: To: "kna101@utdallas.edu" , MSA MICROSCOPY MAILING LIST Message-Id: <8209131001081996/A27033/SRVR05/11A80A8D0800*@MHS> Autoforwarded: false Mime-Version: 1.0 Content-Type: TEXT/PLAIN; CHARSET=US-ASCII Content-Transfer-Encoding: 7BIT Importance: normal Priority: normal Ua-Content-Id: 11A80A8D0800 X400-Mts-Identifier: [;8209131001081996/A27033/SRVR05] Hop-Count: 2 For years, I have taken BEEM capsules, cut the tapered end off with a razor blade, and embedded tissue flat on the 'lid'. After curing the blocks, removing them is easy with a 'cherry pitter' device, which in one catalog the BEEM Company refers to as a 'capsule press'. To remove the cured blocks, simply open the 'lid' of the capsule where the tissue is, place it tissue-side down in the press, and press down on the lever to extricate the block. I find it hard to believe that this is an original idea, but hopefully it will help some of you with flat-embedding dilemmas. Gregg Sobocinski Parke-Davis Pharmaceutical Research Division Ann Arbor, Michigan USA Sobocig@aa.wl.com *** And I'm sure the lawyers would be happy if I include: The views expressed above are my individual opinions and do not represent the views or policies of Parke-Davis. From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-AUG-1996 21:22:03.11 To: MICROARCHIVE CC: Subj: Re: El.diffraction and CCD. From: William Tivol Message-Id: <199608012146.RAA02723@wadsworth.ph.albany.edu> Subject: Re: El.diffraction and CCD. To: lem@paru.cas.cz (Nebesarova LEM Motejl) Date: Thu, 1 Aug 1996 17:46:39 -0400 (EDT) Cc: microscopy@Sparc5.Microscopy.Com In-Reply-To: from "Nebesarova LEM Motejl" at Aug 1, 96 08:24:06 am X-Mailer: ELM [version 2.4 PL23] Content-Type: text Content-Length: 2889 Dear Milos, > We want also to use electron > diffraction on the TEM Philips 420 and to connect CCD or TV camera to > microscop in the near future. And there are some problems to solve. > > Please, I have a few questions. > > 1. Is there some difference in el. diffraction images obtained by > different way? I mean: > a/ Normal image is loaded by camera to PC and the diffraction is > obtained by computer. There are several limitations to resolution using this method. A video camera has a limited pixel size and number--larger and fewer than film--so the Fourier components are inherently fewer than for scanned film. This may or may not be a problem. The intensities are also of limited range, giving possible inaccuracies, and the intensity at one pixel can affect that measured at adjacent pixels. Of course, the contrast transfer function also affects the intensities. Finally, the resolution is limited by the lens aberrations (assuming that pixel size does not impose a more stringent limit). It is, however, very fast. > b/ El.diffraction image is loaded on the planfilm and this one is > processed by laser diffraction. There are some limitations due to lens aberrations and (possibly) grain size. I find typically that this method gives about one more order than the first method. The difference is probably due to the greater sam- pling frequency as a consequence of smaller pixel size. > c/ El. diffraction is loaded directly by camera and processed by computer. The resolution limits are usually much better than with the image. In the very favorable cases, ED can go out to at least 0.05 nm. There can be problems with intensity quantitation due to pixel size and crossover be- tween pixels. The first problem is called "Wooster error"; Inoue's book on video microscopy explains the second type of problem. > d/ El. diffraction is loaded on the planfilm and this one is scanned > to computer and processed by computer. Resolution is equally good, but accurate quantitation requires sensitometry--measuring a blackening curve (OD vs exposure)--which can be tedious. The correct scanner to use is one with a small spot of light, such as the Perkin-Elmer or Optronics. CCD array scanners, such as Eikonix or CCD cameras will not account correctly for very intense spots due to stray light from more transparent areas of the film. This is the most accurate method, and the slowest. > > 2. Exist some camera /CCD or TV/, by which it is possible to load el. > diffraction images directly? Yes. I have seen Ken Downing's camera on the IVEM at Berkeley. / I'am afraid that very intensive main > beam can destroy the detector/ A beam stop can be used to block the beam--I don't know what the consequences are if the beam is not blocked, but I'd be worried too. Yours, Bill Tivol From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-AUG-1996 00:11:48.86 To: MICROARCHIVE CC: Subj: Ground loops and image defects Message-Id: <199608020347.XAA21834@mime2.prodigy.com> X-Mailer: Prodigy Internet GW(v0.9beta) - ae02dm02sc04 From: GVKM07A@PRODIGY.COM (DR CHARLES A GARBER) Date: Thu, 1 Aug 1996 23:47:32, -0500 To: Microscopy@Sparc5.Microscopy.Com Date: 01 Aug 96 To: Microscopy@Sparc5.Microscopy.Com Subject: Ground loops and image defects MIME-Version: 1.0 Content-type: text/plain; charset=us-ascii -- [ From: Charles A. Garber, Ph. D. * EMC.Ver #3.0 ] -- Twice in the past twenty six years we had mysterious problems arise that had all the symptoms of stray AC fields which caused various problems with the images on what were then JEOL JSM U3 SEMs. In one instance in particular, quite a bit of major equipment was brought in (and dollars spent) to find the source of the field but to no avail. The proposed "fixes", and with no guarantees, included extensive "mu" metal shielding of the microscope room. However, by going around and turning off individual circuits in the building, and finding out which "circuit", when "off" caused the problem to go away, in one instance it was traced to a ground feedback loop from a vacuum pump with windings starting to fail. And in the other instance , which was a little more challenging, it was traced to a compressor motor that was part of the microscope itself, with (apparently) the same problem, windings starting to fail on the motor. In both instances, replacing the failing motor (or pump), the problem disappared. So while expensive and complex solutions are sometimes necessary, don't sell short what might be accomplished with the good old common horse sense approach. Chuck ====================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President 1-(800)-2424-SPI SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: GVKM07A@prodigy. com West Chester, PA 19381-0656 USA Customer Service: spi2spi@2spi. com Look for us! ############################ WWW: http://www.2spi.com ############################ ===================================================== From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-AUG-1996 06:14:00.76 To: MICROARCHIVE CC: Subj: Re: Measuring freezing rates Comments: Authenticated sender is From: wesleysm@biology.und.ac.za (James Wesley-Smith) Organization: Biology To: tphillips@biosci.mbp.missouri.edu (Tom Phillips) Date: Fri, 2 Aug 1996 09:01:11 +0200 Subject: Re: Measuring freezing rates CC: Microscopy@Sparc5.Microscopy.Com Priority: normal X-mailer: Pegasus Mail for Windows (v2.01) Message-ID: <19960802133020073.AAA188@WESLEYSM> Hello Tom Thanks a stack for the information. It certainly gives me a good starting point. I am intersted in acquiring a data collecting card, and they obviously vary in speed and frequency of the sampling (and price). I just wish I could keep this sort of information in MY head for 15 years!! Thanks again. ################################################### James Wesley-Smith Electron Microscope Unit George Campbell Building University of Natal Durban, South Africa http://www.und.ac.za/und/emu/emunit.html ################################################### From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-AUG-1996 06:15:37.50 To: MICROARCHIVE CC: Subj: Subscribe Message-Id: Date: Fri, 2 Aug 96 01:11 +0100 From: PLANO@T-ONLINE.DE (PLANO W. Plannet GmbH) X-Sender: 0644197650-0001@t-online.de (PLANO W. Plannet GmbH) Subject: Subscribe To: microscopy@Sparc5.Microscopy.Com Subscribe From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-AUG-1996 08:13:28.97 To: MICROARCHIVE CC: Subj: more on vibes From: "Crossman, Harold" To: microscopy Subject: more on vibes Date: Fri, 02 Aug 96 08:03:00 EDT Message-Id: <32017139@hq_smtp> Encoding: 31 TEXT X-Mailer: Microsoft Mail V3.0 Greetings, We are in the process of tracking down similar problems. One avenue that we are examining is mechanic vibration in the column due to the location of the EDS dewar. The dewar is essentially a large undamped liquid mass hung out to the side of the object that must remain vibration-free. Well the location of the dewar is approximately equidistant to the two adjoining sides of the room. By placing a small mirror over the top of the dewar, we can watch the LN2 shimmer. By moving sound-absorbing foam sheets around the area between the dewar and the walls, we can see a difference in the vibration. We have more work to do on this, but it seems like the dewar is in the "sweet spot" in the room if you were going to set up a stereo system. We are also going to move the 3-phase transformer that sits on the other side of the wall (less than 3 feet from column and console) in the surface analysis lab to get rid of the known electrical vibration. ------------------------------------------------- Harold J. Crossman OSRAM SYLVANIA INC. Lighting Research Center 71 Cherry Hill Dr. Beverly, MA 01915 Phone: (508) 750-1717 E-mail: crossman@rd.sylvania.com Our web sites: www.sylvania.com www.osram.de www.siemens.com From: SMTP%"Karl.Kadler@man.ac.uk" 2-AUG-1996 09:13:34.66 To: MICROARCHIVE CC: Subj: unsuscribe From: Karl Kadler To: microscopy@Sparc5.Microscopy.Com Date: Fri, 2 Aug 1996 14:43:58 BST Subject: unsuscribe Reply-to: Karl.Kadler@man.ac.uk Priority: normal X-mailer: Pegasus Mail/Mac (v2.1.2) Message-ID: <2A24986254E@fs1.sem.man.ac.uk> unsuscribe Karl Kadler, PhD, Wellcome Trust Senior Research Fellow, Wellcome Trust Centre for Cell-Matrix Research, Electron Microscope Unit, School of Biological Sciences, University of Manchester, Stopford Biulding 2.205, Oxford Road, Manchester M13 9PTAssistant: Carol McMurdo (cmcmurdo@fs2.scg.man.ac.uk) From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-AUG-1996 10:15:04.12 To: MICROARCHIVE CC: Subj: Need Zeiss/Leitz Accessories Date: Fri, 2 Aug 1996 07:15:47 -0700 (PDT) Message-Id: <199608021415.HAA24948@zorro.bctel.ca> X-Sender: a1a01963@mail.bctel.ca X-Mailer: Windows Eudora Light Version 1.5.2 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" To: Microscopy@Sparc5.Microscopy.Com From: Ron Neumeyer Subject: Need Zeiss/Leitz Accessories I am looking for the following optics and accessories: (1) Zeiss West dry darkfield condenser with holder Z (0.75/0.85) (2) Zeiss West planopo 25x phase 3 objective (3) Leitz Ortholux nosepiece If you have any of the above in mint condition please advice quoting price, including shipping to Vancouver Canada, in $US. Thank you for your attention in this matter. Regards, Ron Neumeyer phone 604-582-2552 fax 604-623-6239 E-mail (micron@bc.sympatico.ca) From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-AUG-1996 11:13:25.85 To: MICROARCHIVE CC: Subj: Re: Ground loops and image defects Date: Fri, 2 Aug 1996 10:08:51 -0400 (EDT) From: "A. Kent Christensen" X-Sender: akc@tempest.rs.itd.umich.edu To: DR CHARLES A GARBER cc: Microscopy@Sparc5.Microscopy.Com, Microscopy@Sparc5.Microscopy.Com Subject: Re: Ground loops and image defects In-Reply-To: <199608020347.XAA21834@mime2.prodigy.com> Message-ID: MIME-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Sometimes these field effects can be very strange and challenging. Years ago, when I was at Temple Univ. Medical School, in Philadelphia, a colleague in the building had a recently installed Philips 300 TEM that suffered from intermittent image deflection. Things were all right most of the time, but occasionally the image would be deflected off to the side for a time, and would then return to its normal position. The Philips service personnel tried everything, but the cause remained a mystery. It eventually turned out that an elevator in the vicinity had a large iron counterweight that went up and down the shaft all day long. The image deflection occurred when this magnetized mass of iron happened to be at the same level as the microscope. A. Kent Christensen, University of Michigan, akc@umich.edu. From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-AUG-1996 12:14:53.28 To: MICROARCHIVE CC: Subj: unsubscribe Date: Fri, 2 Aug 1996 12:12:27 -0400 (EDT) From: Jun Wang To: microscopy@Sparc5.Microscopy.Com Subject: unsubscribe Message-ID: MIME-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII unsubscribe for vacation ****************************************************************************** Jun Wang g9326479@mcmail.cis.mcmaster.ca Center for Electroptical Materials And Devices/Dept. of Engineering Physics Tel. 905 525 9140 x24936 McMaster University Fax: 905 527 8409 Hamilton, Ontario Canada L8S 4L7 From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-AUG-1996 13:16:48.25 To: MICROARCHIVE CC: Subj: LEO (Zeiss) EM 906 Users Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Fri, 2 Aug 1996 13:16:53 -0500 To: Microscopy@Sparc5.Microscopy.Com From: rml@med.unc.edu (Robert Bagnell) Subject: LEO (Zeiss) EM 906 Users Are there any other EM 906 owners out there who would like to share their experiences with this instrument? From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-AUG-1996 14:15:10.65 To: MICROARCHIVE CC: Subj: RE-Stray EM fields Message-ID: Date: 2 Aug 1996 12:37:30 -0400 From: "Wil Bigelow" Subject: RE-Stray EM fields To: "Micros/EM fields" X-Mailer: Mail*Link SMTP/QM 3.0.0GM Subject: Time: 12:23 PM OFFICE MEMO RE:Stray EM fields Date: 8/2/96 I fully agree with Chuck Garber. A few common sense tests can often clear up the cause of image irregularities without invoking a lot of expensive high-tech methods. We had one instance when the heating element in a furnace in a lab down the hall had shorted over to ground, producing horrendous ground loop currents through the heating ducts and steel girders in the walls of the building that gave periodic image distortion. In another instance, an educational TV station was feeding signals into the building mains that caused problems. However, if all else fails, there is a company (Linear Research Associates, Trumansburg, NY 14886 Fx: 770-368-8256) that specializes in correcting EM field problems. In fact, Curt Dunham, of LRA has recently published a series of articles on EM field problems in "Microscopy Today". You might contact him. From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-AUG-1996 15:15:15.12 To: MICROARCHIVE CC: Subj: More vibes ~ ~ ~ ~ ~ From: Woody.N.White@mcdermott.com Date: 2 Aug 96 13:41:00 -0500 To: microscopy@Sparc5.Microscopy.Com Subject: More vibes ~ ~ ~ ~ ~ Ua-Content-Id: More vibes ~ P1-Recipient: microscopy@sparc5.microscopy.com P1-Message-Id: US*MCI*MCDERMOTT;c\650\960802125903b Original-Encoded-Information-Types: IA5-Text X400-Trace: US*MCI*MCDERMOTT; arrival 960802134100-0500 deferred 960802134100-0500 action Relayed Message-Id: P1-Content-Type: P2 As the operator of a well used Etec, I have encountered worn and loose gears/bearings in the stage. Enviro vibes, which normally did not create a problem, caused "jaggies" as low as 5000x. Etec FYI: My system has been modified by removing the diff pump and installing a turbo. Beware of ball bearing units. The Etec is far too susceptible to their vibrations. I have been using a Leybold maglev pump since they became available and can see no pump induced vibes below about 30Kx. Woody White Babcock & Wilcox Research Ctr. woody.n.white@mcdermott.com woody.white@worldnet.att.net From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-AUG-1996 15:43:27.99 To: MICROARCHIVE CC: Subj: Re: Measuring freezing rates Date: Fri, 2 Aug 1996 09:24:11 -0400 (EDT) From: Sara Miller To: James Wesley-Smith cc: microscopy@Sparc5.Microscopy.Com Subject: Re: Measuring freezing rates In-Reply-To: <19960801210644974.AAA185@WESLEYSM> Message-ID: MIME-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII On Thu, 1 Aug 1996, James Wesley-Smith wrote: > Date: Thu, 1 Aug 1996 16:38:59 +0200 > From: James Wesley-Smith > To: microscopy@Sparc5.Microscopy.Com > Subject: Measuring freezing rates > > I would appreciate to hear from anyone who has had experience in > RECORDING freezing rates used in cryofixation. I am particularly > interested in establishing the duration and interval of the sampling > required. > > Please reply directly to my address. > > Thank you. > > > ################################################### > James Wesley-Smith > Electron Microscope Unit > George Campbell Building > University of Natal > Durban, South Africa > http://www.und.ac.za/und/emu/emunit.html > ################################################### Try contacting Dr.Joe Costello at Dept. of Cell Biology University of North Carolina Chapel Hill, NC 27514 Sorry, I don't have an email address. For faster answers, you might also check your library. He has published info on rates of freezing in different cryogens; he may have described the methods for measuring them. It was probably in the late 1980's. Another possibility is to contact the companies that sell freezing equipment such as slammers, etc. They should be able to give you some references on "how they know their product works." Good luck, S.> Sara E. Miller, Ph. D. P. O. Box 3020 Duke University Medical Center Durham, NC 27710 Ph: 919 684-3452 FAX: 919 684-8735 From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-AUG-1996 16:12:13.23 To: MICROARCHIVE CC: Subj: Re: Venting Print Processors test-header: [jmo@cidmac.wustl.edu] Message-ID: Date: 2 Aug 1996 15:35:30 +0100 From: "Judy Ogilvie" Subject: Re: Venting Print Processors To: "Microscopy Listserver" , "Paula Allan-Wojtas" X-Mailer: Mail*Link SMTP-QM 3.0.2 I am new to the listserver and have missed any recent discussion of print processors. We are likely to buy one in the near future and would appreciate comments on what people have and like. I really liked the old Kodak print processors. They were very fast, easy to use, relatively easy to clean, and had a small footprint on the benchtop. But they are no longer available as far as I can tell. The new ones that I have looked into all seem to be designed for professional labs. Although the prints come out completely fixed and dry, they take a relatively long time which is a pain for test prints. Ours will get moderate and sporadic use so cleaning out the chemicals should be easy. Thanks in advance. jmo ------------------------------------------------ Judith Mosinger Ogilvie, Ph.D. Assistant Research Scientist Central Institute for the Deaf -affiliate of Washington University- 818 S. Euclid Ave. St. Louis, MO 63110 tel: 314-977-0280 fax: 314-977-0030 e-mail: jmo@cidmac.wustl.edu ------------------------------------------------ From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-AUG-1996 16:36:19.42 To: MICROARCHIVE CC: Subj: RE-O-ring sizes Message-ID: Date: 2 Aug 1996 11:58:21 -0400 From: "Wil Bigelow" Subject: RE-O-ring sizes To: "Micros/O-rings" X-Mailer: Mail*Link SMTP/QM 3.0.0GM Subject: Time: 11:49 AM OFFICE MEMO RE:O-ring sizes Date: 8/2/96 Phil: I have always used O-rings in the Parker Series 2 that I obtain from the Zatkoff Co. in Farmington Hills, MI (313-478-2400). These are based on 'nominal' widths which are common fractions of an inch; however, actual widths don't correspond exactly to the nominal ones, and there is some variation probably due to manufacturing variability (it's difficult to measure O-rings exactly, anyway). Here are some of the standard nominal sizes with the quoted corresponding actual dimensions: Nom: 1/16": Act: 1.78 mm, 0.70" 3/32": 2.62 mm, 0.103" 1/8": 3.53 mm, 0.139" 3/16": 5.33 mm, 0.210" 1/4": 6.99 mm, 0.275" For each thickness there is a range of ID sizes available. I have just been designing a couple of devices for Japanese-built TEMs (which one might expect to have metric O-rings, since all other dimensions are metric), and find that all the O-rings used on them fall into this series pretty nicely. Possibly you also can find O-rings from this series that will meet your needs. Zatkoff stocks them in Viton, and several other kinds of rubber. Good luck, Wil Bigelow (bigelow@umich.edu) From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-AUG-1996 17:12:40.08 To: MICROARCHIVE CC: Subj: Re: Measuring freezing rates Date: Fri, 2 Aug 1996 16:38:07 -0500 Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" To: microscopy@Sparc5.Microscopy.Com From: bmenco@casbah.acns.nwu.edu (Bert Menco) Subject: Re: Measuring freezing rates Re. James Wesley-Smith's querie: A good and recent source book: (vacationing) Patrick Echlin, Low Temperature Microscopy and Analysis. Plenum Press, New York , 1992 I think that Joe Costello joined Univ. North Carolina, Chapel Hill Bert Menco Neurobiology & Physiology Hogan Hall Northwestern University Evanston, IL 60208-3520 From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-AUG-1996 17:56:59.37 To: MICROARCHIVE CC: Subj: vibrations Date: Fri, 2 Aug 1996 11:47:47 -0400 (EDT) From: rutledge phil X-Sender: prutle1@umbc8.umbc.edu To: microscopy Subject: vibrations Message-ID: MIME-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Vibration can be a nasty problem. You can't assume they are coming from your area. When I was at George Washington Univ. Med School my lab was on the 5th. floor. Never had problems with vibration until one day I was looking at cilia at 200,000 times and saw it. Zeiss came in to look at the scope and the surrounding area and couldn't find what was causing the problem. They saw vibration only when they put the meter on the desk of the scope. Put it on the floor, no vibration detectable. We looked at another TEM on the floor below us and they had no problem even at 300,000x. After much hunting we found a air handling unit 2 floors below us and about 100ft. away from the scope with a bad rubber foot pad. We had the physical plant dept. replace it and our vibration problem disappeared. Apparently this was causing low level vibration that my scope was picking up and the other scope (floor below) wasn't. Vibration, especially low level can be anywhere, construction several blocks away, bad feet on a centrifuge, new equipment, just about anything. So if you're having a problem with vibration check everything you can, don't rule anything out unless you've checked it out, this includes anything touching your mechanical pumps such as cables, hoses or even boxes you might have set on the backing hoses. Peace, Phil From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-AUG-1996 17:58:50.76 To: MICROARCHIVE CC: Subj: In-Situ protocols Message-Id: Date: Fri, 2 Aug 96 10:39 EDT X-Sender: goldmrkr@pop.fast.net X-Mailer: Windows Eudora Light Version 1.5.2 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" To: microscopy@Sparc5.Microscopy.Com From: "Donald P. Cox" Subject: In-Situ protocols TO: Harry Murray At 11:56 AM 7/30/96 -0230, you wrote: > >Help, > > I am a grad student working on the development of a technique to local >ize "antifreeze" gene expression in winter flounder gill epithelium. So far, >I have been successful in describing the spatial dynamics of expression using >non-isotopic digoxigen probes and a HRP/DAB marker. My problem lies in >attempts to pin point the specific cell types that are expressing. >In the development of my protocoal, I took whole gill filaments and processed >them for insitu hybridization, getting a nice pattern of expression. My next >step was to embed this tissue in resin and cut semi-thin and ultra-thin >sections which were subsequently scoped to theoretically reveal staining in >association with the involved cells. Unfortunately, I cannot detect any diff >erences between experimentals and controls, infact no staining is >detectable at all. The question is, what is happening to the DAB staining >product and why can't I see it in section? Any ideas?? > >H. Murray > I recommend you go to the homepage for Dr. Gwen Childs for in-situ references and protocols. See it at: http://cellbio.utmb.edu/childs Regards, Don Cox ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ * Donald P. Cox, Ph.D. GOLDMARK BIOLOGICALS * * 437 Lock Street Phillipsburg, NJ 08865 * * Telephone: (908) 859-2631 Fax: (908) 859-2875 * * E-Mail: goldmrkr@pop.fast.net or goldmarker@aol.com * * Web Site: http://members.aol.com/goldmarker * * * * "Goldmarking...is fun...and permanent, too!!" * ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-AUG-1996 18:13:57.50 To: MICROARCHIVE CC: Subj: Re: graduate student projects Date: Fri, 2 Aug 1996 12:21:04 -0600 (MDT) From: Mike Folsom To: Jill Craig cc: Microscopy Newsgroup Subject: Re: graduate student projects In-Reply-To: Message-ID: MIME-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII On Thu, 1 Aug 1996, Jill Craig wrote: > > Hi, I'm looking for some ideas of possible projects at the master's > degree level in SEM microscopy. Areas of primary interest would be > Materials Science/Engineering, Environmental, and Biological/Medical. I > know this is an incredibly diverse area. I'd just like to hear some > brainstorming type ideas on things you may have wanted to see > investigated, but time/money/etc. restraints don't allow it. I would be > happy to either post a summary or keep from posting any suggestions as > you prefer. > > Thanks in advance for your ideas. > > > Jill > One thing to consider is JOBS - The market for biological microscopists is fairly bad right now and the salaries for the jobs that are out there aren't much better. On the other hard the job market in materials microscopy seems to be much better and the salaries are probably much higher. No doubt some on the list won't agree with me on this but we would all be better off if the number of skilled microscopists out there was cut. And, the easiest way to do that is to stop training 'em. Michael From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-AUG-1996 19:11:52.55 To: MICROARCHIVE CC: Subj: unsubscribe X-Sender: reid@oj.rsmas.miami.edu Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Fri, 2 Aug 1996 19:57:09 -0500 To: microscopy@Sparc5.Microscopy.Com From: preid@rsmas.miami.edu (Pamela Reid) Subject: unsubscribe unsubscribe __________ Dr. Pamela Reid Research Associate Professor University of Miami/RSMAS-MGG 4600 Rickenbacker Causeway Miami, Fl 33149 email: preid@rsmas.miami.edu phone (305) 361-4606 fax (305) 361-4632 From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-AUG-1996 20:15:21.35 To: MICROARCHIVE CC: Subj: Ultramicrotomy-Hard Materials From: "Andrew Buechele" Organization: Vitreous State Laboratory To: Microscopy@Sparc5.Microscopy.Com Date: Fri, 2 Aug 1996 20:01:06 -0500 EST Subject: Ultramicrotomy-Hard Materials Priority: normal X-Mailer: Pegasus Mail for Windows (v2.42a) Message-Id: <427B1304D8C@rsrch.vsl.cua.edu> I am planning to buy a reconditioned ultramicrotome (since I don't have enough money available for a new unit) and diamond knife in hopes of producing TEM specimens of leached glass. I would appreciate hearing from anyone who has gone the "used instrument" route to ultramicrotomy of hard materials. What kind of success did you have? Are there things to look out for? etc. Thanks in advance. Andy Buechele The Catholic University of America 409 Hannan Hall Washington, D.C. 20064 (202) 319-4995 FAX: (202) 319-4469 From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-AUG-1996 21:14:29.18 To: MICROARCHIVE CC: Subj: Re: O-Rings Date: Fri, 2 Aug 1996 17:49:19 -0500 (CDT) Message-Id: <199608022249.RAA15221@tristero.io.com> X-Sender: ablue@mail.io.com (Unverified) X-Mailer: Windows Eudora Light Version 1.5.2 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" To: Microscopy@Sparc5.Microscopy.Com From: "A. Greene" Subject: Re: O-Rings >Date: Fri, 02 Aug 1996 16:47:36 >To: rutledge phil >From: "A. Greene" >Subject: Re: O-Rings > >Hello Phil, I have been very pleased with American Seal, Inc. as a source for o-rings. They are very responsive and have even overnighted o-rings to my motel when I was out doing microscope service. Great people and excellent stock. Their address is: 1242 Kress Street > Houston, Texas 77020 > Phone 800/527-3151 > FAX 713/675-3618 > > > Alex Greene > Scientific Instrumentation Services, Inc. > Number 499, Post Office Box 19400 > Austin, Texas 78760 > Phone: 512/282-5507 > FAX 512/280-0702 > > >At 02:36 PM 8/1/96 -0400, you wrote: >>I was wondering if anybody knows of a supplier of o-rings in the metric size. >>The suppliers I have dealt with here in Baltimore only have stock of even >>size o-rings such as 2mm, 3mm, etc. I need a supplier that can provide >>fractional sizes also such as, 2.3mm, 2.5mm, etc. I am more concerned >>about the width of the o-ring being fractional than I am of the I.D. or O.D. >>All of the o-rings I use are in whole numbers as far as the O.D. and I.D. >>goes. Thanx. >> >>O.D.O's >> >>Phil >> >> > From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-AUG-1996 21:14:31.54 To: MICROARCHIVE CC: Subj: Re: O-Rings Date: Fri, 2 Aug 1996 17:49:19 -0500 (CDT) Message-Id: <199608022249.RAA15221@tristero.io.com> X-Sender: ablue@mail.io.com (Unverified) X-Mailer: Windows Eudora Light Version 1.5.2 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" To: Microscopy@Sparc5.Microscopy.Com From: "A. Greene" Subject: Re: O-Rings >Date: Fri, 02 Aug 1996 16:47:36 >To: rutledge phil >From: "A. Greene" >Subject: Re: O-Rings > >Hello Phil, I have been very pleased with American Seal, Inc. as a source for o-rings. They are very responsive and have even overnighted o-rings to my motel when I was out doing microscope service. Great people and excellent stock. Their address is: 1242 Kress Street > Houston, Texas 77020 > Phone 800/527-3151 > FAX 713/675-3618 > > > Alex Greene > Scientific Instrumentation Services, Inc. > Number 499, Post Office Box 19400 > Austin, Texas 78760 > Phone: 512/282-5507 > FAX 512/280-0702 > > >At 02:36 PM 8/1/96 -0400, you wrote: >>I was wondering if anybody knows of a supplier of o-rings in the metric size. >>The suppliers I have dealt with here in Baltimore only have stock of even >>size o-rings such as 2mm, 3mm, etc. I need a supplier that can provide >>fractional sizes also such as, 2.3mm, 2.5mm, etc. I am more concerned >>about the width of the o-ring being fractional than I am of the I.D. or O.D. >>All of the o-rings I use are in whole numbers as far as the O.D. and I.D. >>goes. Thanx. >> >>O.D.O's >> >>Phil >> >> > From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 3-AUG-1996 10:16:00.89 To: MICROARCHIVE CC: Subj: Message-Id: <9608031406.AA07892@dibit.hsr.it> Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Sat, 3 Aug 1996 16:11:45 -0300 To: microscopy@Sparc5.Microscopy.Com From: zimarie@dibit.hsr.it (Zimarino Vincenzo) subscribe microscopy vujanam@dibit.hsr.it (Milos Vujanac) From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 3-AUG-1996 11:15:09.55 To: MICROARCHIVE CC: Subj: Message-Id: <9608031403.AA10190@dibit.hsr.it> Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Sat, 3 Aug 1996 16:08:29 -0300 To: microscopy@Sparc5.Microscopy.Com From: zimarie@dibit.hsr.it (Zimarino Vincenzo) "subscribe microscopy" zimarie@dibit.hsr.it Vincenzo Zimarino San Raffaele Scientific Institute DIBIT- Biological and Technological Research Department Room 4-A2-46 Via Olgettina, 58 20132 Milano Italy Tel. + 39 2 26 43 48 96 Fax + 39 2 26 43 48 44 e-mail : zimarie@dibit.hsr.it From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 3-AUG-1996 15:16:48.70 To: MICROARCHIVE CC: Subj: Web Disc Groups Message-ID: Date: 3 Aug 1996 12:28:24 -0800 From: "Murphy, Judy" Subject: Web Disc Groups To: "Microscopy List" X-Mailer: Mail*Link SMTP-MS 3.0.2 We are teaching microscopy and I want to make discussion groups for each of my classes using the internet. I would appreciate anyone's experience (good and bad) in the software they have used and problems that I might encounter. Thanks in advance Judy M. Judy Murphy, PhD Microscopy Technology Center San Joaquin Delta College 5151 Pacific Ave Stockton, CA 95207 Phone: 209/474-5284 FAX: 209/474-5600 e-mail: murphy@sjdccd.cc.ca.us program web page: http://www.sjdccd.cc.ca.us/ElectMicro/sjdc.html From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 3-AUG-1996 18:12:51.97 To: MICROARCHIVE CC: Subj: Re: UNSUBSCIBE Message-ID: <6EC8033201F70300@mhs.unc.edu> In-Reply-To: <66C8033201F70300@mhs.unc.edu> Date: Sat, 3 Aug 1996 18:57:26 -0400 From: "Schoonhoven, Robert" Sender: "Schoonhoven, Robert" Organization: UNC To: microscopy@Sparc5.Microscopy.Com Subject: Re: UNSUBSCIBE MIME-Version: 1.0 Content-type: text/plain; charset="US-ASCII" Content-disposition: inline Content-transfer-encoding: 7BIT X-Mailer: Connect2-SMTP 4.10.rc2 MHS to SMTP Gateway UNSUBSCRIBE From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-AUG-1996 12:52:23.56 To: MICROARCHIVE CC: Subj: Tripod Polisher (R) Workshop Date: 04 Aug 96 12:48:22 EDT From: South Bay Technology <73531.1344@CompuServe.COM> To: Micro Listserver Subject: Tripod Polisher (R) Workshop Message-ID: <960804164822_73531.1344_EHT105-2@CompuServe.COM> REDUCED FEE REGISTRATION DEADLINE AUGUST 31, 1996 Workshop on Tripod Polishing Workshop Objective This course will cover all aspects of pre-thinning and focus on final thinning via Tripod Polishing. Due to the limited class size and the extensive hands-on opportuinities, this course is well suited to novices as well as advanced Tripodders. The course will include sections on: How to do it and why should I? What's really going on and what am I really seeing? How to prepare small, specific area cross-sections. The problem of wildly differing materials (eg tungsten). Rapid preparation of TEM cross-sections. Preparation of a wide range of materials: semiconductors, ceramics, metals,... Hands-on Opportunity This course will be unique in that it will provide a hands-on opportunity for every class participant. Tripod Polishers, Polishing Wheels, and pre-thinning equipment will be made available to all participants and actual samples will be prepared - by the students - as part of the course. This is a great opportunity to get your hands dirty and actually learn by doing. The instructors will walk you through each step of the process and then let you loose on the equipment. This course is designed to teach the Tripod Polishing technique. Silicon samples will be provided to the students and used as the basis for the course teaching. Workshop Location and Dates South Bay Technology - San Clemente, CA Dates: Friday & Saturday - October 18-19, 1996 Previous Participants (partial list) INTEL, AMD, Motorola, LSI Logic, Conner Peripherals, Univ of Maryland, Univ of New Mexico, UNAM (Mexico), LG Electronics (Korea), Battelle, MEMC, MVA Inc., Univ of Michigan, U.S. Bureau of Mines, IBM, Naval Research Lab, Purdue Univ, Univ of Alabama, Univ of Arizona, Univ of Colorado, Univ of Wisconsin. Class Size Due to the intensive hands-on aspects of this course, class size will be strictly limited to 10 participants. Registration Fee: $795 (includes lunches and Friday night Dinner) $695 if registration fee paid by August 31, 1996 Workshop Registration Deadline: 30 days prior to workshop For additional Information: Monica Pflaster South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673 TEL: 800-728-2233 FAX: 714-492-1499 e-mail: sbt@southbaytech.com Registration Form To register for the workshop, please fill out this form and send it, with registration fee to: South Bay Technology, Inc. Workshop on Tripod Polishing 1120 Via Callejon San Clemente, CA 92673 USA Payment must be made in the form of a check, money order, Visa or MasterCard. Checks must be drawn on a U.S. Bank and made payable to South Bay Technology, Inc. Credit card orders by FAX may be sent to South Bay Technology at 714-492-1499. Name: Affiliation: Address: City: State: Zip: Country:_________ Telephone: FAX: e-mail:________________________ Primary sample type: VISA MasterCard Card #_________________________________ Expiration Date________ Signature of Cardholder_________________________ Cardholder name (Please print):________________________________________ From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-AUG-1996 14:53:02.29 To: MICROARCHIVE CC: Subj: OSRAM HBO-lamps From: OSRAMWG@aol.com Date: Sun, 4 Aug 1996 14:58:13 -0400 Message-ID: <960804145813_252565021@emout15.mail.aol.com> To: microscopy@Sparc5.Microscopy.Com Subject: OSRAM HBO-lamps Dear subscribers, first of all, let me introduce myself. My name is Dr. Wolfgang Gottschalk (osramwg@aol.com) and I am the productmanager at OSRAM GMBH headquarters for HBO and low wattage XBO lamps. Last week it has come to my attention, that several people had problems with lamps from our company. Customers satisfaction is our goal and therefore we want to fix existing problems as soon as they have been reported to us and also to support customers if the problem is related to non-OSRAM equipment. However up to now, only two cases have been reported to us. Therefore I like to ask everybody, who had problems with our lamps to report this to me directly. It will help us for the investigation to have the following information: 1.) What was the problem? 2.) What type of lamp was it related to? 3.) In what type of equipment do you use our lamps? Is it modified? 4.) What was the serial number of the lamp (imprinted on the metal base)? 5.) To whom and when did you report the problem (local OSRAM office or mircoscope equipment manufacturer)? Please adress this information to me as soon as possible. We will provide statements on the evaluation process in the microscopy list. Please feel free to contact me in the future directly in case of any question or problems. We feel, that an honest relationsship between manufacturer and the customer is a win-win -relationship for both parties. Best regards Dr. W. Gottschalk From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-AUG-1996 15:53:04.31 To: MICROARCHIVE CC: Subj: Web Disc Groups-More Specific Message-ID: Date: 4 Aug 1996 11:51:04 -0800 From: "Murphy, Judy" Subject: Web Disc Groups-More Specific To: "Microscopy List" X-Mailer: Mail*Link SMTP-MS 3.0.2 From: Murphy, Judy on Sun, Aug 4, 1996 11:49 AM Subject: RE: Web Disc Groups To: Murphy, Judy I sent out a general request for information on setting up discussion groups for my classes on the internet the other day however from my responses I made the question too general, so here is a try at making it more specific: We are teaching microscopy and I want to make discussion groups for each of my classes using the internet. Each of the discussion groups would have a password. Having this on the internet would allow all the students in the classes to get into the discussion whenever they wanted. They would also be using the internet for research searches, etc. I would also like a way to post class assignments etc, i.e. some sub group which the students don't necessarily add anything to. I would appreciate anyone's experience (good and bad) in the software they have used and problems that I might encounter. Thanks in advance Judy M. Judy Murphy, PhD Microscopy Technology Center San Joaquin Delta College 5151 Pacific Ave Stockton, CA 95207 Phone: 209/474-5284 FAX: 209/474-5600 e-mail: murphy@sjdccd.cc.ca.us program web page: http://www.sjdccd.cc.ca.us/ElectMicro/sjdc.html ________________________________________________________ From: Murphy, Judy on Sat, Aug 3, 1996 12:28 PM Subject: Web Disc Groups To: Microscopy List We are teaching microscopy and I want to make discussion groups for each of my classes using the internet. I would appreciate anyone's experience (good and bad) in the software they have used and problems that I might encounter. Thanks in advance Judy M. Judy Murphy, PhD Microscopy Technology Center San Joaquin Delta College 5151 Pacific Ave Stockton, CA 95207 Phone: 209/474-5284 FAX: 209/474-5600 e-mail: murphy@sjdccd.cc.ca.us program web page: http://www.sjdccd.cc.ca.us/ElectMicro/sjdc.html From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-AUG-1996 17:50:57.52 To: MICROARCHIVE CC: Subj: Re: graduate student projects Message-ID: <320546A2.1360@vicon.net> Date: Sun, 04 Aug 1996 17:56:02 -0700 From: John Best Organization: ELMDAS Co. X-Mailer: Mozilla 2.01 (Win16; U) MIME-Version: 1.0 To: mwfolsom@UNM.EDU CC: jcraig@unbc.edu, microscopy@Sparc5.Microscopy.Com Subject: Re: graduate student projects References: Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Michael Folsom recently wrote: One thing to consider is JOBS - The market for biological microscopists is fairly bad right now and the salaries for the jobs that are out there aren't much better. On the other hard the job market in materials microscopy seems to be much better and the salaries are probably much higher. No doubt some on the list won't agree with me on this but we would all be better off if the number of skilled microscopists out there was cut. And, the easiest way to do that is to stop training 'em. End of Mr. Folsoms reply. To Mr. Folsoms reply: I apologize in advance to the subscribers of this listserver for responding to an issue of this nature, but your response is disgusting, Mr. Folsom. In my view the Scanning Electron Microscope has been one of the primary instruments contributing to the advancement of our economy, technology, and the ability to raise the standard of living for all people. Advancing the utilization of the SEM into new areas of research is essential to our future. We need more people trained in the fundamental physics which govern the limitations and possibilities for this instrument. We also need people to excercize their imagination with respect to the huge number of problems the SEM can be utilized to solve. Ultimately this will create more jobs. Respectfully John W. Best. From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-AUG-1996 23:50:32.09 To: MICROARCHIVE CC: Subj: comparison with TEM and SEM techinques (fwd) Date: Mon, 05 Aug 1996 13:35:45 +1000 (EST) From: VINCENT CHAND Subject: comparison with TEM and SEM techinques (fwd) To: microscopy@Sparc5.Microscopy.Com Cc: v.chand@student.qut.edu.au Message-id: MIME-version: 1.0 Content-type: TEXT/PLAIN; charset=US-ASCII Content-transfer-encoding: 7BIT I need some information to general procedures w.r.t. prep. to specimens to be studied under either TEM or SEM techinques. Can anyone help me with me in this regard, as I am relative new to some of these procedures. Basically I would like to any new techniques used to prepare specimens for SEM and TEM. TIA From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 5-AUG-1996 02:52:33.29 To: MICROARCHIVE CC: Subj: Re: graduate student projects Date: Mon, 5 Aug 1996 00:44:17 -0600 (MDT) From: Mike Folsom To: microscopy@Sparc5.Microscopy.Com Subject: Re: graduate student projects Message-ID: MIME-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII >> Michael Folsom recently wrote: >> One thing to consider is JOBS - >> >> The market for biological microscopists is fairly bad right now and the >> salaries for the jobs that are out there aren't much better. >> >> On the other hard the job market in materials microscopy seems to be >> much better and the salaries are probably much higher. >> >> No doubt some on the list won't agree with me on this but we would all >> be better off if the number of skilled microscopists out there was cut. >> And, the easiest way to do that is to stop training 'em. > End of Mr. Folsoms reply. > > To Mr. Folsoms reply: > I apologize in advance to the subscribers of this listserver for > responding to an issue of this nature, but your response is disgusting, > Mr. Folsom. > > In my view the Scanning Electron Microscope has been one of the primary > instruments contributing to the advancement of our economy, technology, > and the ability to raise the standard of living for all people. > Advancing the utilization of the SEM into new areas of research is > essential to our future. We need more people trained in the > fundamental physics which govern the limitations and possibilities for > this instrument. We also need people to excercize their imagination with > respect to the huge number of problems the SEM can be utilized to solve. > > Ultimately this will create more jobs. > > Respectfully > John W. Best. I'm really not interested in letting this whole thing get personal - I posted my reply because I thought a discussion about the job market for microscopists would be a good thing. A few thoughts late Sunday night.................. First, let's deal with the real - not the ideal. The simple fact is that all kinds of wonderful microscopy needs to be done ranging from basic paraffin work to SEM, TEM, & LSCM. However, that doesn't mean that the system is gonna provide the funds or opportunity to do the work. Hasn't anybody noticed that microscopy labs are being closed down or simply allowed to decay all over the place. At a time when all kinds of incredible technological developments are occurring in biological microscopy it seems to be dying right before our eyes. To blindly advise a student to follow us into the abyss is immoral. The job market in the sciences stinks. And, as I hear it - its worse in Physics than in Biology. So, what do we gain by telling somebody to "follow us" when we can't even see a future for ourselves. Isn't this the ultimate act of selfishness? To advise a student to "do what we did" and not care about their future? Are they just sacrifical lambs on the alter of science? A cheap and willing pair of hands to exploit for our personal good? Should they be told to give up all matter of things for an uncertain future - all in the cause of science! Can't we do better than that? Frankly, there will always be the need for a good microscopist to be handy. But as a technician - not as a collegue. In a University setting that automatically means a second class citizen with a third rate pay scale. Why should we wish that on our students, our young collegues? As, I said earlier I posted my original reply to start a discussion. We really have no need for silly polemics. Michael From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 5-AUG-1996 05:58:29.65 To: MICROARCHIVE CC: Subj: Message-Id: <1.5.4.32.19960805094453.0066cce4@mail.pi.se> X-Sender: o906001@mail.pi.se X-Mailer: Windows Eudora Light Version 1.5.4 (32) Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Mon, 05 Aug 1996 11:44:53 +0200 To: microscopy@Sparc5.Microscopy.Com From: Henrik Fermin subscribe henrik.fermin@fujifilm.se **************************************************************************** Henrik Fermin Phone: + 46 (08) 729 14 57 FUJI FILM SVERIGE AB Technical Manager BAS Fax: + 46 (08) 33 71 29 Box 23086 Medical Imaging System Dept. 104 35 Stockholm SWEDEN ***************************************************************************** From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 5-AUG-1996 08:00:30.23 To: MICROARCHIVE CC: Subj: unsuscribe Mime-Version: 1.0 Date: Mon, 5 Aug 1996 07:42:16 -0400 Message-Id: <205DEF80.1322@inet.spri.sp.com> From: NEAL.SHARPE@spcorp.com (NEAL SHARPE) Subject: unsuscribe To: microscopy@Sparc5.Microscopy.Com Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7bit Content-Description: cc:Mail note part Unsuscribe Thanks!! From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 5-AUG-1996 08:57:25.58 To: MICROARCHIVE CC: Subj: RE: comparison with TEM and SEM techniques (fwd) From: "Marti, Jordi" To: Microscopy Subject: RE: comparison with TEM and SEM techniques (fwd) Date: Mon, 05 Aug 96 09:32:00 EDT Message-Id: <3205F825@crlpc1.research.allied.com> Encoding: 45 TEXT X-Mailer: Microsoft Mail V3.0 TIA: The thing to remember is that electrons do not travel very far in solid materials ( order of magnitude of 100 nm , but this will depend on voltage and the material at hand). In SEM, where one is interested in studying the "surface" of the specimen (e.g. topographic features), we do not have to worry about getting a "thin" (electron transparent) specimen. In this case therefore, the techniques used are aimed at getting a "representative surface". In TEM on the other hand, the electrons need to travel through the specimen and be able to form an image bellow the specimen. Therefore, getting thin, electron transparent specimens is critical to TEM. I would suggest that you start by checking some of the text books on SEM and TEM. Two that come to mind (but certainly not the only ones) are "Techniques For Electron Microscopy " edited by D.Kay Blackwell Scientific Publications LTD 1965 and "Scanning Electron Microscopy and X-Ray Microanalysis" by Goldstein et . al. Both have extensive discussions on sample preparation techniques. For some newer techniques (e.g. tripod polishing) and specific stains for polymers and biological materials you will have to go to appropriate scientific journals. Have Fun Jordi Marti ---------- From: Microscopy-request To: microscopy Cc: v.chand Subject: comparison with TEM and SEM techinques (fwd) Date: Monday, August 05, 1996 1:35PM I need some information to general procedures w.r.t. prep. to specimens to be studied under either TEM or SEM techinques. Can anyone help me with me in this regard, as I am relative new to some of these procedures. Basically I would like to any new techniques used to prepare specimens for SEM and TEM. TIA From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 5-AUG-1996 09:49:38.57 To: MICROARCHIVE CC: Subj: Re: graduate student projects From: "Marti, Jordi" To: Microscopy Subject: Re: graduate student projects Date: Mon, 05 Aug 96 10:27:00 EDT Message-Id: <32060504@crlpc1.research.allied.com> Encoding: 22 TEXT X-Mailer: Microsoft Mail V3.0 Somehow I think we digressed from Jill's original request, but here are my thoughts on the issue of the Microscopy Job market. No I don't think that they (the students) " .. are just sacrificial lambs on the alter of science? As long as we make it clear to them (i.e. the younger generations) that the microscopy field, while exciting as it is , is not a passport to a secure job. I do meet too many scientist and engineers who are literally bored with their jobs even though they went into the field at a time when things looked quite promising. I still believe that finding a carrier that is exciting should be stressed just as much as the pitfalls of the job market. It is my impression that in today's industrial environment, scientists and engineers ( of all ranks !) are being asked to perform a number of tasks which are clearly outside of their core area of training. Today, employees must be flexible enough to adjust to these changes. We might do better if we see our own fields (microscopy or whatever) as a means rather than as ends in themselves. My advice: If microscopy truly excites you, go into it heartily, but be prepared to accept changes later on. Jordi Marti From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 5-AUG-1996 12:45:42.17 To: MICROARCHIVE CC: Subj: Re: Web Disc Groups-More Specific/Resources Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Mon, 5 Aug 1996 09:54:55 -0500 To: (MSA Listserver)Microscopy@sparc5.microscopy.com From: Peters@BSAC.UCHC.EDU (Klaus-Ruediger Peters) Subject: Re: Web Disc Groups-More Specific/Resources Although hypertext provides for microscopy the attractive benefit of combining images with text, nothing useful for discussion groups is yet available. This summary comes from the following URLs: "Update Notes for Conferencing on the Web" http://freenet.msp.mn.us/~drwool/webconup.html. A large list of free and commercial resources with short evaluations and demo sites are at: "Computer Conferencing on the Web (Discussion Forums and Groupware)" http://freenet.msp.mn.us/~drwool/webconf.html#wbb Does anyone has experience with any of the programs cited in the above URs? Best regards, Klaus ******************* >I want to make discussion groups for each of my >classes using the internet. Each of the discussion groups would have a >password. Having this on the internet would allow all the students in the >classes to get into the discussion whenever they wanted. They would also be >using the internet for research searches, etc. I would also like a way to >post class assignments etc, i.e. some sub group which the students don't >necessarily add anything to. ****************** ****************************************************************************** * Klaus-Ruediger Peters, Ph.D. :Molecular Imaging Laboratory * * Director, Molecular Imaging Laboratgory : http://panda.uchc.edu/ * * Biomolecular Structure Analysis Center : htklaus/index.html * * University of Connecticut Health Center : * * 263 Farmington Ave. :F r e e Access to Differential * * Farmington, CT 06030-2017; U.S.A :Contrast Software at * * e-Mail: Peters@BSAC.UCHC.EDU : http://panda.uchc.edu/ * * Tel: (860) 679-3977; Fax: (860) 679-1989 : htklaus/DHP-Img.html#Software* ****************************************************************************** From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 5-AUG-1996 12:51:30.43 To: MICROARCHIVE CC: Subj: POSTDOCTORAL POSITION OPEN Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Mon, 5 Aug 1996 09:25:44 -0500 To: Microscopy@Sparc5.Microscopy.Com From: kdj928@lulu.acns.nwu.edu (Kevin Johnson) Subject: POSTDOCTORAL POSITION OPEN Cc: vpdravid@casbah.acns.nwu.edu ================================================================================ POSTDOCTORAL POSITION OPEN - IMMEDIATELY !! ANALYTICAL ELECTRON MICROSCOPY/MATERIALS SCIENCE Qualifications: A PhD in Materials Science/Physics or related area. Strong experimental and conceptual background in interface structure and crystal defect phenomena, especially in oxide materials is necessary. Experience in oxide superconductors is desirable but not required. Extensive hands-on experience in advanced analytical EM (XES, EELS, CBED etc..) is a must, including TEM specimen preparation of complex oxides, especially cross-sections of thin films. The position is a part of a multi-faceted research program concerned with structure-property correlation for oxide interfaces. The research would involve experimental and phenomenological studies of oxide interfaces in electroceramics, including high Tc compounds and ferroelectric thin films. The principal theme is to exploit high spatial resolution analytical techniques to probe the crystallography, chemistry and aspects of electronic structure associated with internal interfaces in these functional materials to complement ongoing atomistic simulation and ab-initio electronic structure calculations of interfaces. Instrumentation available at NU in the newly restructured Electron Probe Instrumentation Center (EPIC) includes: A Hitachi HF-2000 FEG TEM/STEM with x-ray, EELS, in-situ IV/LHe holder and e- holography set-up, a Hitachi S4500-II FEG SEM with x-ray, EBSP/OIM and LHe stage with in-situ IV probes, a Hitachi H8100 cTEM, and access to various other TEMs/SEMs and other analytical instrumentation. The position is open immediately for at least one year, and renewable upon mutual consent. Northwestern University is an equal opportunity employer. ******************************************************* (Vinayak P. Dravid) Associate Professor, Materials Science & Engineering Director, Electron Probe Instrumentation Center (EPIC) 2225 N. Campus Drive, MLSF Building Northwestern University, Evanston, IL 60208, USA Ph.: (847) 467-1363, Fax: (847) 491-7820 E-mail: v-dravid@nwu.edu ******************************************************* From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 5-AUG-1996 15:37:53.44 To: MICROARCHIVE CC: Subj: Re: graduate student projects Date: Mon, 05 Aug 1996 15:11:13 -0500 From: hou@kcgl1.eng.ohio-state.edu (Vincent D.H. Hou) Subject: Re: graduate student projects To: Microscopy@sparc5.microscopy.com Message-id: MIME-version: 1.0 Content-type: text/plain; charset="us-ascii" Content-transfer-encoding: 7BIT >>John W. Best recently wrote: > > In my view the Scanning Electron Microscope has been one of the primary > instruments contributing to the advancement of our economy, technology, > and the ability to raise the standard of living for all people. > Advancing the utilization of the SEM into new areas of research is > essential to our future. We need more people trained in the > fundamental physics which govern the limitations and possibilities for > this instrument. We also need people to excercize their imagination with > respect to the huge number of problems the SEM can be utilized to solve. > > Ultimately this will create more jobs. >> Michael Folsom recently wrote: > >First, let's deal with the real - not the ideal. The simple fact is that >all kinds of wonderful microscopy needs to be done ranging from basic >paraffin work to SEM, TEM, & LSCM. However, that doesn't mean that >the system is gonna provide the funds or opportunity to do the work. > >Hasn't anybody noticed that microscopy labs are being closed down or simply >allowed to decay all over the place. At a time when all kinds of incredible >technological developments are occurring in biological microscopy it seems to >be dying right before our eyes. > >To blindly advise a student to follow us into the abyss is immoral. The job >market in the sciences stinks. And, as I hear it - its worse in Physics than >in Biology. So, what do we gain by telling somebody to "follow us" when we >can't even see a future for ourselves. Isn't this the ultimate act of >selfishness? To advise a student to "do what we did" and not care about >their future? Are they just sacrifical lambs on the alter of science? A >cheap and willing pair of hands to exploit for our personal good? Should >they be told to give up all matter of things for an uncertain future - all in >the cause of science! > >Can't we do better than that? > >Frankly, there will always be the need for a good microscopist to be handy. >But as a technician - not as a collegue. In a University setting that >automatically means a second class citizen with a third rate pay scale. Why >should we wish that on our students, our young collegues? > I would like to throwing in my two cents here: Real or Ideal? This is a difficult question to answer. It may well be the only question needed to be answered for your life. So what should we tell these young/bright graduate students? The truth. I belive that every one who has been doing research, either in basic or applied research in any discipline, would agree that it is a very interesting and exciting thing to do. I also believe that the excitment may also be worn out with the increasing pressure to satisfy the "real" need of life. So, new graduate students should be well informed before they make their OWN decision. It's definitely unfair just to tell the one-side story. I am still tring to find a balance between "real" and "ideal". Vincent From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 5-AUG-1996 15:51:25.09 To: MICROARCHIVE CC: Subj: Re: graduate student projects Message-ID: <32067D26.516E@vicon.net> Date: Mon, 05 Aug 1996 16:00:54 -0700 From: John Best Organization: ELMDAS Co. X-Mailer: Mozilla 2.01 (Win16; U) MIME-Version: 1.0 To: mwfolsom@UNM.EDU CC: microscopy@Sparc5.Microscopy.Com Subject: Re: graduate student projects References: Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Mr. Folsom, Nothing personal was intended, and I certainly didn't imply that students should follow blindly. I simply don't see the present and future of SEM as bleak in the slightest. Salaries for microscopists might not be what you or I would like them to be. If a student of microscopy derives their feelings of self worth from their salary, then perhaps they shouldn't be looking at microscopy as a profession. On the other hand, the majority of jobs in microscopy are with universities and serious corporations. Perhaps salaries are (arguably) low, but other important benefits are provided. For now, I'll stick with my opionion that SEM based R&D at the university level will ultimately expand the possibilities for electron beam applications in industry, hence creating more opportunities. Go for it graduate students. You might not want to be a microscopist when you come out into industry, but the SEM will provide you with a unique view of what makes things tick. The understanding that goes along with this vantage point is invaluable to potential employers. Regards and Good Luck to all. John Best. From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 5-AUG-1996 17:12:32.32 To: MICROARCHIVE CC: Subj: Re: graduate student projects Message-Id: <199608051433.JAA27943@ux1.cso.uiuc.edu> Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Mon, 5 Aug 1996 09:39:20 -0500 To: microscopy@sparc5.microscopy.com From: oshel@ux1.cso.uiuc.edu (philip oshel) Subject: Re: graduate student projects >Can't we do better than that? > >Frankly, there will always be the need for a good microscopist to be handy. >But as a technician - not as a collegue. In a University setting that >automatically means a second class citizen with a third rate pay scale. Why >should we wish that on our students, our young collegues? > > >As, I said earlier I posted my original reply to start a discussion. We >really have no need for silly polemics. > > >Michael My $0.02: John Best's idealism is laudable and easily defensible. Unfortunately Michael Folsom has a much more realistic view of the world. With one exception: it's the techs who are getting cut. I speak from personal experience, having had 2 jobs now shot out from under me by administrators who are more interested in personal advancement than it serving the university community. The problems of microscopy labs being starved into extinction and closed is very real, and the only things now that prevent it is either lots of press coverage or powerful administrators/full faculty who need microsopy for their own purposes. It all comes down to education: educating administrators, legislators, and faculty about the value and need of all forms of microscopy. Until that happens, Michael Folsom's comments must be taken very seriously. Too many students are being trained without regard for whether or not they will be able to find any position, much less a good one. Phil &&&&&&&&&&& Illigitmi non carborundum &&&&&&&&&&& Philip Oshel University of Illinois Rm 74 Bevier Hall 905 S. Goodwin Ave. Urbana, IL 61801 (217) 244-3145 oshel@ux1.cso.uiuc.edu *********** looking for a job again *********** From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 5-AUG-1996 23:49:47.92 To: MICROARCHIVE CC: Subj: electron microscopy of bacteriophage Date: Tue, 06 Aug 1996 14:30:10 +1000 (EST) From: VITHAGNA KHAMMANIVONG Subject: electron microscopy of bacteriophage To: microscopy@Sparc5.Microscopy.Com Message-id: MIME-version: 1.0 Content-type: TEXT/PLAIN; charset=US-ASCII Content-transfer-encoding: 7BIT Could you please send me book references or journal references on how to process and view bacteriophage in a bacterial culture. From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-AUG-1996 00:50:47.65 To: MICROARCHIVE CC: Subj: electron microscopy of bacteriophage Date: Tue, 06 Aug 1996 14:40:05 +1000 (EST) From: VITHAGNA KHAMMANIVONG Subject: electron microscopy of bacteriophage To: microscopy@sparc5.microscopy.com Cc: v.khammanivong@student.qut.edu.au Message-id: MIME-version: 1.0 Content-type: TEXT/PLAIN; charset=US-ASCII Content-transfer-encoding: 7BIT Could you please send me any book references or journal references on how to process and view bacteriophage in a bacterial culture. From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-AUG-1996 01:55:24.07 To: MICROARCHIVE CC: Subj: Preparation of mucosal parasites Date: Tue, 06 Aug 1996 14:39:36 +1000 (EST) From: MATTHEW JOHNSTON Subject: Preparation of mucosal parasites To: microscopy@Sparc5.Microscopy.Com Cc: m.johnston@qut.edu.au Message-id: MIME-version: 1.0 Content-type: TEXT/PLAIN; charset=US-ASCII Content-transfer-encoding: 7BIT Does anyone know of any good books on how to prepare giardiasis for the electron microscope or on how to prepare a mucosal parasite, or on waterborne parasites? Thanks. From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-AUG-1996 02:55:30.84 To: MICROARCHIVE CC: Subj: Re: graduate student prospects Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Tue, 6 Aug 1996 00:07:21 -0700 To: John Best , microscopy@Sparc5.Microscopy.Com From: em@mediacity.com (Ed Monberg) Subject: Re: graduate student prospects >Mr. Folsom, > >Nothing personal was intended, and I certainly didn't imply that students >should follow blindly. I simply don't see the present and future of SEM >as bleak in the slightest. Dear Group, A small point if I may: What is the time constant for changes in the practitioner/demand ratio for a given profession such as microscopy which has a longish "gestation" or training period ? Judging from other fields with similar training periods, the cycle is shortest where the variables are strictly economic, such as in the various branches of science, teaching and engineering. However where some organization, the AMA or national bar association for example, twiddles the process through lobbying, propaganda, disinformation in the marketplace, or manipulation of demand for services, it appears that the cycles are much longer, and the swings much deeper. I vote for letting INFORMED self-interest do the strolling through the yellow pages. Ed Monberg Palo Alto From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-AUG-1996 03:55:09.38 To: MICROARCHIVE CC: Subj: FREE Tripod Polisher Tutorial at MSA Date: 06 Aug 96 01:20:51 EDT From: South Bay Technology <73531.1344@CompuServe.COM> To: Micro Listserver Subject: FREE Tripod Polisher Tutorial at MSA Message-ID: <960806052051_73531.1344_EHT125-4@CompuServe.COM> As part of the Microscopy & Microanalysis sponsored Exhibitor Tutorial Program, South Bay Technology will be presenting a tutorial on Tripod Polishing for SEM and TEM Analysis. Date: Tuesday, August 13 Time: 5pm - 6pm Location: Microscopy & Microanalysis Exhibit Hall South Bay Technology Booth #500 Registration: Please register at the MSA Education Committee Booth on the exhibit floor. There is NO CHARGE for the tutorial. If you need any additional information about the tutorial, please check the MSA Web Site at: http://www.msa.microscopy.com or contact me directly. The first 10 people who confirm their registration by visiting the South Bay Technology booth will receive a FREE Tripod Polisher (r) T-shirt! Best regards- David Henriks TEL: 800-728-2233 (toll-free in USA) South Bay Technology, Inc. 714-492-2600 1120 Via Callejon FAX: 714-492-1499 San Clemente, CA 92673 USA e-mail: henriks@southbaytech.com http://www.southbaytech.com Manufacturers of Precision Sample Preparation Equipment and Supplies for Metallography, Crystallography and Electron Microscopy. From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-AUG-1996 04:36:00.91 To: MICROARCHIVE CC: Subj: Date: Tue, 6 Aug 1996 00:20:34 -0400 From: simkin@egr.msu.edu (Benjamin - Simkin) Message-Id: <199608060420.AAA01491@mulder> To: Microscopy@Sparc5.Microscopy.Com Subject: Microscopy in grad research As further contribution to the debate at hand: I don't believe at all that one must make the choice to "be a microscopist" or "not be a microscopist"; I am currently finishing up my Master's on some applications of a microscopy techneque to certian materials science type problems. This could certianly place me within the catagory of "microscopist" from a number of standpoints, but I have no intention of limiting myself to only one field of work in the future. I am of the opinion that most people pursuing an advanced degree in materials science should be able to use well such a versitile analytical tool, and know the means behind the information that they are aquiring; otherwise the microscope fails to provide all the information possible, and of the questions that might be profitably asked, some would go unseen. While I believe a good technician can (and should) come up with and answer a number of useful questions for most any sample, it remains impossible to have anyone other than the researcher in question to ask all that should be asked. Furthermore, without a passible working knowledge of the principals of the type of microscopy being employed, the researcher won't even know of the possible methods of answering those questions that emerge during examination. A minimum requirment would therefore seem to include knowledge of: the vacuum system; beam-sample interactions, especially those that produce exploitable contrast; and the exploitable signals arising from the above. Throw in there rudimentary operation skills for the most common techniques, and one would seem to have a microscopist, if perhaps not of terribly high skill. I would therefore urge anyone in graduate study to at least examine projects dealing with microscopy, as it will likely prove useful later on, and is unlikely to hurt. Ben Simkin (simkin@egr.msu.edu) Dept. Mat. Sci. & Mech. Michigan State University o previously some stuff that has been said included: >So what should we tell these young/bright graduate students? The truth. I >belive that every one who has been doing research, either in basic or >applied research in any discipline, would agree that it is a very >interesting and exciting thing to do. I also believe that the excitment may >also be worn out with the increasing pressure to satisfy the "real" need of >life. So, new graduate students should be well informed before they make >their OWN decision. It's definitely unfair just to tell the one-side story. > >Vincent > > >Salaries for microscopists might not be what you or I would like them to >be. If a student of microscopy derives their feelings of self worth from >their salary, then perhaps they shouldn't be looking at microscopy as a >profession. On the other hand, the majority of jobs in microscopy are >with universities and serious corporations. Perhaps salaries are >(arguably) low, but other important benefits are provided. > >For now, I'll stick with my opionion that SEM based R&D at the university >level will ultimately expand the possibilities for electron beam >applications in industry, hence creating more opportunities. Go for it >graduate students. You might not want to be a microscopist when you come >out into industry, but the SEM will provide you with a unique view of >what makes things tick. The understanding that goes along with this >vantage point is invaluable to potential employers. > >John Best. From: SMTP%"loraamm@chuma.cas.usf.edu" 6-AUG-1996 08:57:45.55 To: MICROARCHIVE CC: Subj: Message-ID: <32074E26.246D@chuma.cas.usf.edu> Date: Tue, 06 Aug 1996 08:52:38 -0500 From: Betty Loraamm Reply-To: loraamm@chuma.cas.usf.edu X-Mailer: Mozilla 2.02 (Macintosh; I; 68K) MIME-Version: 1.0 To: microscopy@Sparc5.Microscopy.Com Subject: X-URL: http://www.biotech.ufl.edu/icbr/emcl/db/Microsrv.html Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit subscribe microscopy loraamm@chuma.cas.usf.edu From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-AUG-1996 09:59:38.50 To: MICROARCHIVE CC: Subj: old address Message-Id: From: Stanley Hayes To: "'microscopy @ sparc5.microscopy.com'" Subject: old address Date: Tue, 6 Aug 1996 10:16:24 -0400 X-Mailer: Microsoft Exchange Server Internet Mail Connector Version 4.0.993.5 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Content-Transfer-Encoding: 7bit Please unsubscribe old address and subscribe me at this new address. Thank you. The new address is Stanley_Hayes@NIH.GOV From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-AUG-1996 10:51:23.83 To: MICROARCHIVE CC: Subj: Re: graduate student projects From: "Franklin Bailey" Organization: University of Idaho To: mwfolsom@UNM.EDU, John Best Date: Tue, 6 Aug 1996 08:24:57 PST8PDT MIME-Version: 1.0 Content-type: text/plain; charset=US-ASCII Content-transfer-encoding: 7BIT Subject: Re: graduate student projects CC: microscopy@Sparc5.Microscopy.Com Priority: normal X-mailer: Pegasus Mail for Windows (v2.33) Message-ID: <12DA17B30EE@birch.csrv.uidaho.edu> Date: Mon, 05 Aug 1996 16:00:54 -0700 From: John Best Organization: ELMDAS Co. To: mwfolsom@UNM.EDU Cc: microscopy@Sparc5.Microscopy.Com Subject: Re: graduate student projects Mr. Folsom, Nothing personal was intended, and I certainly didn't imply that students should follow blindly. I simply don't see the present and future of SEM as bleak in the slightest. Salaries for microscopists might not be what you or I would like them to be. If a student of microscopy derives their feelings of self worth from their salary, then perhaps they shouldn't be looking at microscopy as a profession. On the other hand, the majority of jobs in microscopy are with universities and serious corporations. Perhaps salaries are (arguably) low, but other important benefits are provided. For now, I'll stick with my opionion that SEM based R&D at the university level will ultimately expand the possibilities for electron beam applications in industry, hence creating more opportunities. Go for it graduate students. You might not want to be a microscopist when you come out into industry, but the SEM will provide you with a unique view of what makes things tick. The understanding that goes along with this vantage point is invaluable to potential employers. Regards and Good Luck to all. John Best. I have been following this string with much interest, but have vowed not to include my opinion; until now. I have been in electron microscopy since the late '60s, both as a graduate student and "technician". For the past 12 years I have taught two TEM and one SEM class per year at the university level, although I am still classified as an EM Technician. Mr. Folsom is correct in his realistic approach to the subject. We all would enjoy working in our "ideal" jobs and "ideal" situations, but those things only exist at Disneyland. Mr. Best suggests a different occupation if salary is the main concern of the technician. We have all had the dream of being involved in the research which will improve the lot of mankind, but try raising a family on the salary of an EM technician and see how long it takes for reality to set in. In closing, dear graduate students, use electron microscopy as it was intended to be used. It is a high tech tool and should be considered a means to an end, and not the end itself. Franklin Bailey From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-AUG-1996 15:46:31.77 To: MICROARCHIVE CC: Subj: Final Course Announcement From: "Hard, Robert" To: "microscopy.post" Subject: Final Course Announcement Date: Tue, 06 Aug 96 14:30:00 PDT Message-ID: <3207C967@ubmed.buffalo.edu> Encoding: 64 TEXT X-Mailer: Microsoft Mail V3.0 Course Announcement Title: Optical Microscopy and Imaging in the Biomedical Sciences When: October 23 - October 30, 1996 Where: Marine Biology Laboratory, Woods Hole, MA, USA Tuition: $1850 (Includes room and board) Application Deadline: August 13, 1996 Admission application and information: Carol Harnel, Admissions Coordinator Marine Biological Laboratory 7 MBL Street Woods Hole, MA 02543-1015 (508) 289-7401 Internet: admissions@mbl.edu WWW: http://www.mbl.edu Course Director: Colin S. Izzard, State University of New York @ Albany Phone: [518] 442 - 4367 EMail: csizzard@csc.albany.edu Course Description: For Whom: Designed primarily for research scientists, physicians, postdoctoral trainees and advanced graduate students in animal, plant, medical and material sciences. Non-biologists seeking a comprehensive introduction to microscopy and video-imaging will benefit greatly from this course as well. There are no specific prerequisites, but an understanding of the basic principles of optics is desirable. Limited to 24 students. The eight day course consists of lectures, laboratory demonstrations, exercises and discussions that will enable the participant to obtain and interpret microscope images of high quality, to perform quantitative optical measurements, and to produce photographic and video records for documentation and analysis. Topics to be covered include: principles of microscope design and image formation bright field, dark field, phase contrast, differential interference contrast, interference reflection, and fluorescence microscopy confocal scanning microscopy and image deconvolution digital image restoration and 3-D reconstruction video imaging, recording, enhancement, and intensification analog and digital image processing and analysis fluorescent probes and ratio-imaging laser tweezers and laser scissors Applications to live cells will be emphasized; other specimens will be covered as well. Students will have direct hands-on experience with state-of-the-art microscopes, video cameras, recorders and image processing equipment provided by major optical and electronics companies. Instruction will be provided by experienced staff from universities and industry. Students are encouraged to bring their own biological (primary cultures, cell lines, etc.) and material specimens and to discuss individual research problems with the faculty. From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-AUG-1996 16:54:13.95 To: MICROARCHIVE CC: Subj: Durst S-45 Enlarger available Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Tue, 6 Aug 1996 15:04:32 -0700 To: microscopy@Sparc5.Microscopy.Com From: chandler@lamar.ColoState.EDU (John Chandler) Subject: Durst S-45 Enlarger available A friend of mine has a Durst Laborator S-45 enlarger available for sale. The package includes 4 condenser lenses and 5 Schneider Componon lenses of various focal lengths, as well as point light source. Please contact me directly for further details. Serious inquiries only. John chandler@lamar.ColoState.EDU From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-AUG-1996 17:52:10.88 To: MICROARCHIVE CC: Subj: Tracor NS-880 parts Date: Tue, 06 Aug 1996 16:54:15 -0500 (CDT) Date-warning: Date header was inserted by ARWEN.UTHSCSA.EDU From: "Nancy K.R. Smith" Subject: Tracor NS-880 parts X-Sender: smithn@arwen.uthscsa.edu To: microscopy@Sparc5.Microscopy.Com Message-id: <01I7YO37BMYS90OJ5K@ARWEN.UTHSCSA.EDU> MIME-version: 1.0 X-Mailer: Windows Eudora Light Version 1.5.2 Content-type: text/plain; charset="us-ascii" Content-transfer-encoding: 7BIT Microscopy list: Does anyone happen to have a cassette tape transport for a Tracor-Northern NS-880 x-ray analysis system (vintage 1975-1979)? If you do, or know of anyone who does, please contact me directly at smithn@uthscsa.edu. Thanks From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-AUG-1996 18:50:06.47 To: MICROARCHIVE CC: Subj: Your "Ask-a-microscopist" query Message-Id: <199608062109.OAA21994@mail.mcn.org> X-Sender: schooley@mail.mcn.org Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Tue, 6 Aug 1996 14:20:12 -0700 To: biology_team@hopkins.k12.mn.us (Terri Carlson) From: schooley@mcn.org (Caroline Schooley) Subject: Your "Ask-a-microscopist" query Cc: Microscopy@Sparc5.Microscopy.Com, (Zaluzec) Terri - I think that you'll find the size information that you want at: http://www.uq.oz.au/nanoworld/images_1.html Although the images don't have scale bars, the image index gives enough info to calculate them, and the micrographs themselves are excellent. Caroline Schooley Educational Outreach Coordinator, Microscopy Society of America Box 117 Caspar, CA 95420 Phone/FAX (707)964-9460 Email schooley@mcn.org From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-AUG-1996 19:52:07.65 To: MICROARCHIVE CC: Subj: Enlarger_advice? Message-ID: Date: 6 Aug 1996 16:14:46 -0500 From: "Fermin, Cesar" Subject: Enlarger_advice? To: "EM Users (MSA)" X-Mailer: Mail*Link SMTP-MS 3.0.2 Please respond within the next 30 minutes on source and specs for the latest 4x5 enlarger (Omega, Besseler, etc.) Thanks ********************************************************************* *Cesar D. Fermin, Ph.D \|T|/ Fax (504) 587-7389 * *Tulane Medical School /|M|\ Voice Mail (504) 584-2618 * *Pathology/SL79 \|C|/ Secretary (504) 584-2436 * *New Orleans La 70112-2699 /|*|\ Lab/Techn. (504) 584-2521 * * ---->Prof. Pathology & Otolaryngology * *http://www1.omi.tulane.edu/departments/pathology/fermin/cdftop.html* ********************************************************************* From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-AUG-1996 20:53:29.04 To: MICROARCHIVE CC: Subj: Re: Graduate Student Projects Date: Tue, 6 Aug 96 18:59:01 EDT From: "Ed J. Basgall" Message-Id: <9608062259.AA22908@imager.mri.psu.edu> To: Microscopy@Sparc5.Microscopy.Com Subject: Re: Graduate Student Projects Yes, another 2 cents from here...... Not to dwell on career path changes but after spending some 15 years wearing many biological microscopy hats as; graduate student, Lab Director, Senior Microscopist, Director, Res. Associate..... It seems that in order to succeed one might be advised to view microscopy more as a tool and less as a discipline. This has always been the position taken by many of my mentors. What really seems to be most important is that one studies a particular organism or process rather than the technology to do so. Pursuing the study of technology seems to fall more into the realm of analytical and engineering sciences than biological sciences. Of course, it also helps to learn how to write grants and become independently funded. This can provide some shielding against administrators and deans who may only see an EM tech/coordinator as an expensive salary. Yes, education will be a key issue in our future. Ed Basgall, PhD Dept. of Chemistry Penn State University State College, PA 16802 From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 7-AUG-1996 00:19:42.26 To: MICROARCHIVE CC: Subj: Precipitation and evaporation of fluoronanogold label X-Sender: st004110@brandywine.otago.ac.nz Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable Date: Wed, 7 Aug 1996 16:43:57 +1200 To: Microscopy@Sparc5.Microscopy.Com From: kathryn.jones@stonebow.otago.ac.nz (Kathryn R. Jones) Subject: Precipitation and evaporation of fluoronanogold label Hi, I have been using fluoronanogold. I aliquoted out the first 0.2ml I received into eppendorf tubes and cryotubes and placed them in a 4 degrees celcius fridge (10 or 20=B5l aliquots). A number of the aliquots have precipitated or completely evaporated or ended up on the lids of the tubes. I have checked that the temperature of the fridge remains constant. Has anyone got any suggestions for the storage of this antibody? Thanks for your assistance Kathryn From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 7-AUG-1996 00:48:03.41 To: MICROARCHIVE CC: Subj: New SEM images to amuse y'all Date: Tue, 6 Aug 1996 19:14:34 -1000 (HST) From: Tina Carvalho X-Sender: tina@halia To: Microscopy Newsgroup Subject: New SEM images to amuse y'all Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Aloha, Microscopists- I'm looking forward to seeing many of you next week at the MSA meeting! I felt guilty for not preparing a poster this year (the data weren't cooperating), so I spent some time putting together a web page, instead. These images are a product of the imaginations of a recently re-met old high-school friend and myself; an eccentric artist and a mad scientist (='MicroAngelo'). We hope to come up with some more fun stuff soon ('SEMantics'). Check it out at http://www.pbrc.hawaii.edu/bemf/microangelo.html See you in Minneapolis! Tina *************************************************************************** * Tina (Weatherby) Carvalho * tina@pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu * *************************************************************************** http://www.pbrc.hawaii.edu/bemf/microangelo.html From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 7-AUG-1996 02:49:15.46 To: MICROARCHIVE CC: Subj: recording moving sceneries from a Display X-Sender: koster@spinne.biochem.mpg.de Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Wed, 7 Aug 1996 09:24:41 +0200 To: Microscopy@Sparc5.Microscopy.Com From: koster@biochem.mpg.de (Bram Koster) Subject: recording moving sceneries from a Display Dear microscopists I am looking for a system capable of recording moving sceneries shown on a Silicon Graphics workstation display. My impression is that there are two basic approaches: (1) based on recording the sequence of displayed images over an anolog/digital/anaolog converter to a S-VHS videorecorder, and (2) by burning the digital results after various file-conversions into a CD-ROM. For practical purposes the S-VHS approach seems to be more practical/faster, although the CD-ROM approach in combination with an interactive CD-player seems to have the future..... Suggestions on the pros and cons of possibles approaches/systems are very welcome. ------------------------------------------- Dr. Abraham J. Koster Max-Planck-Institute for Biochemistry Department for Structural Biology Am Klopferspitz 18A, D-82152 Martinsried Germany voice : +49-89-8578 2632 fax : +49-89-8578 2641 email : koster@biochem.mpg.de www : http://www/baumeister/koster.html ------------------------------------------- From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 7-AUG-1996 08:54:57.65 To: MICROARCHIVE CC: Subj: Re: recording moving sceneries from a Display Date: Wed, 7 Aug 96 08:50:01 EDT From: "Nathan O'Connor" Message-Id: <9608071250.AA19771@ipl.rpi.edu> To: Microscopy@Sparc5.Microscopy.Com, koster@biochem.mpg.de Subject: Re: recording moving sceneries from a Display Hello, > Dear microscopists > > I am looking for a system capable of recording moving sceneries shown on a > Silicon Graphics workstation display. > > My impression is that there are two basic approaches: > (1) based on recording the sequence of displayed images over an > anolog/digital/anaolog converter to a S-VHS videorecorder, and I've used SGI's "Indy Video" video board for this. Basically, you display your movie on the screen using some kind of animation software (ImageMagick's animate is free) and then place a capture window around the animation. Whatever is being displayed in the capture window can be directed through the board to your recorder. I was satisfied with the quality of the recordings. There are a nice set of signal controls for fine tuning. It did take some experimenting to find the most appropriate settings though. I think that the Indy's ship with VINO video which can only read in video signals. I am not sure about other SGI models. There is also the Galileo video board which I haven't used. Good Luck, Nathan From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 7-AUG-1996 12:00:38.06 To: MICROARCHIVE CC: Subj: EnlargerGothanks Message-ID: Date: 7 Aug 1996 09:19:06 -0500 From: "Fermin, Cesar" Subject: EnlargerGothanks To: "EM Users (MSA)" X-Mailer: Mail*Link SMTP-MS 3.0.2 I found out that replacing the two Besseler enlarger we now have with current models (except Durst which I do not want) would not add any more functionality, specially for the price of newer models. Thanks to those who responded so quickly. I was given 45 minutes to make a decision on $$$$. From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 7-AUG-1996 14:02:47.96 To: MICROARCHIVE CC: Subj: Fluorgold Date: Wed, 7 Aug 1996 11:42:38 -0500 Message-Id: <199608071642.LAA08566@Sparc5.Microscopy.Com> X-Sender: derby@mail.pb.net X-Mailer: Windows Eudora Light Version 1.5.2 Mime-Version: 1.0 Content-Length: 244 Conten