From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-JUN-1996 12:31:09.05 To: MICROARCHIVE CC: Subj: UNSUBSCRIBE Message-ID: <31B07426.74C8@cpcug.org> Date: Sat, 01 Jun 1996 11:47:34 -0500 From: "John M. Libert" Organization: Capital PC User Group X-Mailer: Mozilla 2.0 (Win16; I) MIME-Version: 1.0 To: microscopy@Sparc5.Microscopy.Com Subject: UNSUBSCRIBE Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit UNSUBSCRIBE From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-JUN-1996 14:21:51.94 To: MICROARCHIVE CC: Subj: Microlumina camera Message-Id: <31B03164.416E@sierra.net> Date: Sat, 01 Jun 1996 12:02:44 +0000 From: "Marc C. Brande, MS, Founder" Organization: Cultured Cell Systems X-Mailer: Mozilla 2.0 (Macintosh; U; 68K) Mime-Version: 1.0 To: "List: Confocal" Cc: microscopy@Sparc5.Microscopy.Com Subject: Microlumina camera Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Would anyone have contact information for the Microlumina camera for hi res digital imaging? Thanks so much Marc From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-JUN-1996 06:46:12.47 To: MICROARCHIVE CC: Subj: Re: Microlumina camera Date: Sun, 2 Jun 1996 21:08:58 +1000 Message-Id: <199606021108.VAA22136@ultra.ultra.net.au> X-Sender: pns@mailhost.ultra.net.au X-Mailer: Windows Eudora Light Version 1.5.2 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" To: microscopy@Sparc5.Microscopy.Com From: Probing & Structure Subject: Re: Microlumina camera At 12:02 01-06-96 +0000, you wrote: >Would anyone have contact information for the Microlumina camera for >hi res digital imaging? Thanks so much > >Marc > >That digital camera is available in North America through Electron Microscopy Sciences (email: sgk@aol.com). We are the distributor in Australasia. Jim Darley Manager Probing & Structure Microscopy Supplies & Accessories Phone +61 77 740 370 Fax: +61 77 892 313 Internet Catalogue: http://www.ultra.net/~pns/ From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-JUN-1996 16:34:56.65 To: MICROARCHIVE CC: Subj: Microscopy & Microanalysis-96 Search Engines Running Message-Id: <199606022034.PAA01952@Sparc5.Microscopy.Com> Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Sun, 2 Jun 1996 15:39:49 -0500 To: Microscopy@Sparc5.Microscopy.Com From: zaluzec@Sparc5.Microscopy.Com (Nestor J. Zaluzec) Subject: Microscopy & Microanalysis-96 Search Engines Running Colleagues... A Search Engine has now been implemented for the Scientific Program of Microscopy & Microanalysis-96 . You may search this database by Author Last Name or Keywords selected from the Manuscript Title. The Search Engine is on-line at the Microscopy Society of America's WWW site found at the URL: http://www.msa.microscopy.com In addition, Abstracts of the Contents of Volume 2 Issues 1&2 of the Journal of the Microscopy Society of America are also now on-line, along with a few other new items which you might find interesting. As always please report problems that you might find directly to me.... Cheers.. Nestor Your Friendly Neighborhood SysOp (also Microscopy & Microanalysis-96 Program Chairman) From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-JUN-1996 20:26:03.45 To: MICROARCHIVE CC: Subj: Re: Microscope booking software To: microscopy@sparc5.microscopy.com From: m.dickson@unsw.edu.au (melvyn dickson) Subject: Re: Microscope booking software Date: Mon, 3 Jun 1996 10:04:15 cc: m.dickson@unsw.edu.au Message-ID: In article <31AEFEDF.55DD@jagunet.com> Lawrence Kordon writes: >Date: Fri, 31 May 1996 09:14:55 -0500 >From: Lawrence Kordon >Subject: Re: Microscope booking software >alwyn eades wrote: >> >> We currently have users book microscope time by writing with a pen on >> booking sheets made of paper. We are contemplating changing this antiquated >> system to a computer booking system which would permit people to book (and >> to check existing bookings) over the net. >> >> Does anyone know of software to do this? Please take into account that we >> would want the software to meet the following conditions: >> >> It should work for several instruments. >> It should prevent one user erasing another's booking. >> It should allow staff to change the bookings of users. >> If a user deletes his or her own booking, a record of the booking and the >> time of the cancellation should be kept. >> It should be possible to program booking rules into the system. These rules >> may be different for each instrument. The rules would include such things >> as how many sessions may be booked at any one time and how long a session >> may be booked. >> It should be possible to include various degrees of access for the >> users. Some users may be allowed to book evenings and weekends while others >> may only book daytime sessions. >> We implemented our NET=BOOK software to do precisely this about 4 years ago. It was custom witten in C++ by our Chris Martinic and runs under DOS on our network. It effortlessly handles bookings for 4 EMs, microtomes, coating units, image processors, analysers, and so on. It carries out the functions you prescribe. As it is custom made it can be easily modified when further good ideas are proposed. Chris is off sick today but I predict he will be back on deck in about 7 days and would be ready to field further questions. mel dickson unsw. From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 3-JUN-1996 09:28:43.11 To: MICROARCHIVE CC: Subj: TEM ultra microtomes Posted-Date: Mon, 3 Jun 1996 09:31:16 -0400 Date: Mon, 3 Jun 1996 09:26:04 -0400 (EDT) From: Sally Shrom To: microscopy Subject: TEM ultra microtomes Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII What was the conclusion about the best ultra microtome for ultra thin sectioning of tissue embedded in epon? Sally From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 3-JUN-1996 10:26:02.11 To: MICROARCHIVE CC: Subj: gold workshop From: hainfeld@genome1.bio.bnl.gov Date: Mon, 3 Jun 1996 10:22:50 -0400 Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" To: microscopy@Sparc5.Microscopy.Com Subject: gold workshop Gold Cluster Labeling Workshop (and STEM microscopy) Sept. 18-21, 1996 Brookhaven National Lab Upton, Long Island, NY This is a hands-on lab (and lecture) course that covers labeling proteins with Nanogold and Undecagold. Topics included: labeling chemistry and strategies, labeling thiols and amines, thiol reduction, column chromatography for separating unreacted gold, spectral quantitation/stoichiometry of labeling, preparation of STEM and TEM samples, use of low Z stains, STEM and TEM visualization, silver enhancement of blots, running and detecting labeled proteins on gels, and new labels. Some participants' samples will also be labeled. Instructors: James Hainfeld, Richard Powell (Nanoprobes), Fred Furuya (Nanoprobes), Joseph Wall (BNL), Martha Simon (BNL); guest presentations from successful users Cost: a registration fee of $500 to cover costs (includes materials, dinners, coffee, evening refreshments).. Housing: Dorms at $14.50 per night; or guest house $57/night. Registration limited due to lab equipment; please indicate preliminary interest by June 6, giving name(s) of those that would like to attend. If sufficient interest, additional courses will follow. The Brookhaven STEM is a NIH Biotechnology Resource. Respond by June 6 to: Jim Hainfeld, tel. 516-344-3372, fax. 516-344-3407, email: hainfeld@genome1.bio.bnl.gov (the #1 in genome1) From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 3-JUN-1996 11:39:07.77 To: MICROARCHIVE CC: Subj: LM: pre-embedded fluorescent marker Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Mon, 3 Jun 1996 09:14:10 -0800 To: microscopy@sparc5.microscopy.com From: sbarlow@sunstroke.sdsu.edu (Steve Barlow) Subject: LM: pre-embedded fluorescent marker howdy all I have a user with lung tissue that has been labelled with a fluorescent marker in vivo. She wants to examine light microscope slices (e.g., cryostat slices) for distribution of the marker. I believe most fixatives containing Glutaraldehyde or formadehyde will quench the marker fluorescence and/or add autofluorescence. Any suggestions on how to prepare such tissue? Can we simply plungefreeze small pieces in LN or propane? Can we infiltrate the unfixed tissue first with sucrose as a cryoprotectant? The air pockets will no doubt cause additional problems. Would a quick exposure to a vacuum help the sucrose or fixative penetrate? This request is a new one for me. Any and all suggestions would be appreciated steve Dr. Steven Barlow EM Facility/Biology Dept. San Diego State University San Diego, CA 92182-4614 phone: (619)594-4523 fax:(619)594-5676 email:sbarlow@sunstroke.sdsu.edu From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 3-JUN-1996 14:22:27.80 To: MICROARCHIVE CC: Subj: Databases Date: Mon, 3 Jun 1996 14:12:11 -0400 (EDT) From: Marilyn Wadsworth To: Microscopy@Sparc5.Microscopy.Com Subject: Databases Message-ID: MIME-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Hello all, We are interested in learning what kind of database people are finding most successful for billing purposes and workload recording. We are currently using a Paradox (DOS) which is networked between our imaging facility and business office. Our billing tends to be complicated since we have multiple users, budget numbers and equipment as well as multiple people inputting information and we are looking for an efficient way to streamline our operations. Any comments would be greatly appreciated, either to me directly or to the list. Regards, Marilyn +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ + Marilyn Wadsworth | Phone: (802) 656-0813 + + Cell Imaging Facility | E-Mail: mwadswort@moose.uvm.edu + + School of Medicine, Path Dept | + + University of Vermont | + + Burlington, Vermont 05405 | + +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 3-JUN-1996 15:32:18.76 To: MICROARCHIVE CC: Subj: please post this announcement From: hainfeld@genome1.bio.bnl.gov Date: Mon, 3 Jun 1996 14:59:30 -0400 Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" To: microscopy@Sparc5.Microscopy.Com Subject: please post this announcement Gold Cluster Labeling Workshop (and STEM microscopy) Sept. 18-21, 1996 Brookhaven National Lab Upton, Long Island, NY This is a hands-on lab (and lecture) course that covers labeling proteins with Nanogold and Undecagold. Topics included: labeling chemistry and strategies, labeling thiols and amines, thiol reduction, column chromatography for separating unreacted gold, spectral quantitation/stoichiometry of labeling, preparation of STEM and TEM samples, use of low Z stains, STEM and TEM visualization, silver enhancement of blots, running and detecting labeled proteins on gels, and new labels. Some participants' samples will also be labeled. Instructors: James Hainfeld, Richard Powell (Nanoprobes), Fred Furuya (Nanoprobes), Joseph Wall (BNL), Martha Simon (BNL); guest presentations from successful users Cost: a registration fee of $500 to cover costs (includes materials, dinners, coffee, evening refreshments).. Housing: Dorms at $14.50 per night; or guest house $57/night. Registration limited due to lab equipment; please indicate preliminary interest by June 6, giving name(s) of those that would like to attend. If sufficient interest, additional courses will follow. The Brookhaven STEM is a NIH Biotechnology Resource. Respond by June 6 to: Jim Hainfeld, tel. 516-344-3372, fax. 516-344-3407, email: hainfeld@genome1.bio.bnl.gov (the #1 in genome1) From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 3-JUN-1996 16:25:25.06 To: MICROARCHIVE CC: Subj: Billing software From: "Crossman, Harold" To: "'Microscopy'" Subject: Billing software Date: Mon, 03 Jun 96 16:28:00 PDT Message-Id: <31B36EFC@hq_smtp> Encoding: 7 TEXT X-Mailer: Microsoft Mail V3.0 Might I suggest a small business accounting package? They are set up for just such situations. Many packages contain business templates that can be modified to fit your specific needs. Two that come to mind are Peachtree Accounting and QuickBooks. From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 3-JUN-1996 17:20:05.41 To: MICROARCHIVE CC: Subj: 2 Video Cameras on Same Port Message-Id: <31B2F066.58C8@sierra.net> Date: Mon, 03 Jun 1996 14:02:14 +0000 From: "Marc C. Brande, MS, Founder (619) 587-4830 FAX: (619) 552-1516" Organization: Cultured Cell Systems (Hands-On Cultured Cell Biology/Imaging at Your Site) X-Mailer: Mozilla 2.0 (Macintosh; U; 68K) Mime-Version: 1.0 To: "List: Microscopy" Cc: confocal@ubvm.cc.buffalo.edu, nih-image@soils.umn.edu Subject: 2 Video Cameras on Same Port Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Is it possible to put 2 video cameras on the same port on any of the research grade scope makes (Leica, Zeiss, Nikon, Olympus) so one can easily switch between cameras for different lite mode capture? Thanks in advance. Marc From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 3-JUN-1996 18:21:18.23 To: MICROARCHIVE CC: Subj: LM - Help on In Vivo/Situ Stains for Confocal Message-Id: <199606032253.AA108452380@pigseye.mmm.com> X-Mailer: Windows Eudora Version 1.4.3 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Mon, 03 Jun 1996 17:57:10 -0500 To: Microscopy@Sparc5.Microscopy.Com From: kszaruba@MMM.COM (Karen S. Zaruba) Subject: LM - Help on In Vivo/Situ Stains for Confocal My request is similar, yet perhaps opposite to Dr. Barlow's [regarding specimen preparation for tissue which has been fluorescently tagged in vivo]. We would like to use laser scanning confocal microscopy to visualize the 3D architecture of blood vessels in tissue whole mounts; then section the same tissue for further immunohistochemistry. Does anyone know of a method to label the vessels for confocal that would be compatible with "routine" histological specimen preparation (i.e. formalin fixation and paraffin embedding)? Perhaps there are non-fluorescent tracers (minerals or reflective substances?) that would stand up to the processing?? Your help and ideas are appreciated! Karen Zaruba Life Sciences Sector Lab Reply: kszaruba@mmm.com 3M Company 3M Center 270-1S-01 Phone: 612-737-2971 St. Paul, MN 55144-1000 These opinions are my own and may not represent those of 3M. From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 3-JUN-1996 19:20:22.98 To: MICROARCHIVE CC: Subj: EPMA Room Temp ??? From: Terry.R.McCue@mcdermott.com Date: 3 Jun 96 16:33:00 -0500 To: microscopy@Sparc5.Microscopy.Com Subject: EPMA Room Temp ??? Ua-Content-Id: EPMA Room Temp ? P1-Recipient: microscopy@msa.microscopy.com P1-Message-Id: US*MCI*MCDERMOTT;c\650\960603154551c Original-Encoded-Information-Types: IA5-Text X400-Trace: US*MCI*MCDERMOTT; arrival 960603163300-0500 deferred 960603163300-0500 action Relayed Message-Id: P1-Content-Type: P2 Is there anyone out there who has experience or data regarding the ambient temperture variations that can be tolerated by an ARL Electron microprobe (with AMI " computer, scaler motor upgrade") ie. " counts per degree per hour " or something like that. Recent demands in responce to ISO and our instruments people who keep an eye on this kind of stuff has made it an issue in the writing of our lab Tech. Procedures. THANKS Terry R. McCue Babcock & Wilcox Research 1562 Beeson St. Alliance, Ohio 44601 voice: (330) 829-7427 Fax : (330) 829-7831 internet: terry.r.mccue@rdd.mcdermott.com From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 3-JUN-1996 20:20:47.88 To: MICROARCHIVE CC: Subj: Re: LM: pre-embedded fluorescent marker Date: Mon, 3 Jun 1996 16:47:24 -0500 (CDT) From: tom moninger Subject: Re: LM: pre-embedded fluorescent marker To: Steve Barlow cc: microscopy@Sparc5.Microscopy.Com In-Reply-To: Message-ID: MIME-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII On Mon, 3 Jun 1996, Steve Barlow wrote: > howdy all > > I have a user with lung tissue that has been labelled with a fluorescent > marker in vivo. She wants to examine light microscope slices (e.g., > cryostat slices) for distribution of the marker. I believe most fixatives > containing Glutaraldehyde or formadehyde will quench the marker > fluorescence and/or add autofluorescence. Any suggestions on how to > prepare such tissue? > > Can we simply plungefreeze small pieces in LN or propane? > > Can we infiltrate the unfixed tissue first with sucrose as a > cryoprotectant? The air pockets will no doubt cause additional problems. > Would a quick exposure to a vacuum help the sucrose or fixative penetrate? > > > This request is a new one for me. Any and all suggestions would be appreciated > > steve > > Dr. Steven Barlow > EM Facility/Biology Dept. > San Diego State University > San Diego, CA 92182-4614 > phone: (619)594-4523 > fax:(619)594-5676 > email:sbarlow@sunstroke.sdsu.edu > Freshly prepared formaldehyde should not give you any autofluorescence or quench the probe to any great extent. I have had good luck fixing probes such as various fluo-dextrans, lectins, some membrane dyes, etc. Lung is often a pain to cryo-section. I'd start with the easiest, a PF fixed piece of tissue, freeze it in OCT and section. Hope it works Tom Thomas Moninger (moninger@emiris.iaf.uiowa.edu) Central Microscopy Research Facility 85 EMRB University of Iowa Iowa City, IA 52242 319-335-8142 FAX 319-335-8049 From: SMTP%"glenmac@u.washington.edu" 3-JUN-1996 20:55:22.01 To: MICROARCHIVE CC: Subj: Re: LM: pre-embedded fluorescent mar Date: Mon, 3 Jun 1996 11:39:46 -0700 (PDT) From: Glen Macdonald Reply-To: Glen Macdonald To: Steve Barlow Cc: microscopy@Sparc5.Microscopy.Com Subject: Re: LM: pre-embedded fluorescent mar In-Reply-To: Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Steve, The effect of fixation will depend upon the actual fluorophore employed. There are several which survive formaldehyde fixation. Formaldehyde autofluorescence hasn't been that big of an issue for us. Glut can be a problem in the green/yellow emission range but not so bad in longer wavelengths. If her particular needs dictate, you might try Carnoy's fluid or a methanolic version. The shrinkage is considerable, but it penetrates well and allows fairly good morphology. Hopefully, you have some tissue for trials. Good luck, Glen MacDonald Research Scientist Hearing Research Laboratories of the Virginia Merrill Bloedel Hearing Research Center Box 35-7923 University of Washington Seattle, WA 98195-7923 (206) 616-4156 glenmac@u.washington.edu On Mon, 3 Jun 1996, Steve Barlow wrote: > howdy all > > I have a user with lung tissue that has been labelled with a fluorescent > marker in vivo. She wants to examine light microscope slices (e.g., > cryostat slices) for distribution of the marker. I believe most fixatives > containing Glutaraldehyde or formadehyde will quench the marker > fluorescence and/or add autofluorescence. Any suggestions on how to > prepare such tissue? > > Can we simply plungefreeze small pieces in LN or propane? > > Can we infiltrate the unfixed tissue first with sucrose as a > cryoprotectant? The air pockets will no doubt cause additional problems. > Would a quick exposure to a vacuum help the sucrose or fixative penetrate? > > > This request is a new one for me. Any and all suggestions would be appreciated > > steve > > Dr. Steven Barlow > EM Facility/Biology Dept. > San Diego State University > San Diego, CA 92182-4614 > phone: (619)594-4523 > fax:(619)594-5676 > email:sbarlow@sunstroke.sdsu.edu > > > From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 3-JUN-1996 21:21:15.86 To: MICROARCHIVE CC: Subj: Re: LM: pre-embedded fluorescent marker From: MELLIOTT@prl.pulmonary.ubc.ca Organization: UBC Pulmonary Research Lab To: microscopy@Sparc5.Microscopy.Com Date: Mon, 3 Jun 1996 12:48:44 +0800PST Subject: Re: LM: pre-embedded fluorescent marker Priority: normal X-mailer: Pegasus Mail/Mac (v2.1.2) Message-ID: <1C9738108FA@prl.pulmonary.ubc.ca> Date: Mon, 3 Jun 1996 09:14:10 -0800 To: microscopy@sparc5.microscopy.com From: sbarlow@sunstroke.sdsu.edu (Steve Barlow) Subject: LM: pre-embedded fluorescent marker howdy all I have a user with lung tissue that has been labelled with a fluorescent marker in vivo. She wants to examine light microscope slices (e.g., cryostat slices) for distribution of the marker. I believe most fixatives containing Glutaraldehyde or formadehyde will quench the marker fluorescence and/or add autofluorescence. Any suggestions on how to prepare such tissue? Can we simply plungefreeze small pieces in LN or propane? Can we infiltrate the unfixed tissue first with sucrose as a cryoprotectant? The air pockets will no doubt cause additional problems. Would a quick exposure to a vacuum help the sucrose or fixative penetrate? This request is a new one for me. Any and all suggestions would be appreciated steve Dr. Steven Barlow EM Facility/Biology Dept. San Diego State University San Diego, CA 92182-4614 phone: (619)594-4523 fax:(619)594-5676 email:sbarlow@sunstroke.sdsu.edu We deal with lung tissue all the time in our lab. We inflate the lung through the bronchus/trachea using 50% OCT (Cryomatrix) compound in normal saline as a cryoprotectant. We attach a syringe filled with the OCT to the trachea/bronchus and inject the stuff down the bronchial tree. We then freeze the lung over liquid nitrogen (if you submerge the lung in liquid nitrogen, the cryoprotectant expands and the lung cracks). We store the samples in - 70 freezer and then section on a cryostat. We fix the sections in acetone for 10 minutes prior to staining. We sometimes have problems with autoflourescence but depending on the wavelength they are using it can be done away with (don't use fluorescein). If you need any other info please contact me. Mark Elliott, PhD UBC-Pulmonary Research Laboratory, Vancouver BC Canada From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-JUN-1996 01:23:05.32 To: MICROARCHIVE CC: Subj: ink analysis From: "ROBIN CROSS" Organization: Rhodes University To: microscopy@Sparc5.Microscopy.Com Date: Tue, 4 Jun 1996 07:43:47 GMT+0200 Subject: ink analysis Priority: normal X-mailer: Pegasus Mail v3.22 Message-ID: <2F0FA82423@giraffe.ru.ac.za> Good morning all! Does anyone know of a method for determining age-related changes (years or tens of years, not hundreds or thousands of years) in inks, i.e. compositional or any other changes? Best regards on a bright but very chilly South African morning! Robin Cross Director : EM Unit, Rhodes University, Grahamstown, South Africa eurc@giraffe.ru.ac.za (tel: +27 461 318168 - fax: +27 461 24377) From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-JUN-1996 07:25:25.66 To: MICROARCHIVE CC: Subj: re: ink analysis From: "Crossman, Harold" To: "'Microscopy'" Subject: re: ink analysis Date: Tue, 04 Jun 96 07:04:00 PDT Message-Id: <31B4383A@hq_smtp> Encoding: 26 TEXT X-Mailer: Microsoft Mail V3.0 One source of information may be: McCrone Research Institute 2820 S. Michigan Ave. Chicago, IL 60616-3292 USA (312) 842-7100 fax (312) 842-1078 They have extensive experience with particles, forensics, paints, pigments, etc. I have no financial interest, etc. in McCrone. ------------------------------------------------- Harold J. Crossman OSRAM SYLVANIA INC. Lighting Research Center 71 Cherry Hill Dr. Beverly, MA 01915 Phone: (508) 750-1717 E-mail: crossman@rd.sylvania.com Our web site: www.sylvania.com From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-JUN-1996 08:25:33.69 To: MICROARCHIVE CC: Subj: Re: Databases Date: Tue, 4 Jun 1996 08:26:26 GMT Message-Id: <199606040826.IAA18543@snitch.biotech.ufl.edu> X-Sender: sdw@biotech.ufl.edu X-Mailer: Windows Eudora Light Version 1.5.2 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" To: Marilyn Wadsworth From: Scott Whittaker Subject: Re: Databases Cc: microscopy@Sparc5.Microscopy.Com At 02:12 PM 6/3/96 -0400, you wrote: >Hello all, >We are interested in learning what kind of database people are finding >most successful for billing purposes and workload recording. We are >currently using a Paradox (DOS) which is networked between our imaging >facility and business office. Our billing tends to be complicated since >we have multiple users, budget numbers and equipment as well as multiple >people inputting information and we are looking for an efficient way to >streamline our operations. Any comments would be greatly appreciated, >either to me directly or to the list. > > Regards, > Marilyn > > +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ > + Marilyn Wadsworth | Phone: (802) 656-0813 + > + Cell Imaging Facility | E-Mail: mwadswort@moose.uvm.edu + > + School of Medicine, Path Dept | + > + University of Vermont | + > + Burlington, Vermont 05405 | + > +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ > > There was a similar discussion on the list recently which has been archived at the www address at the end of this message. Click on "Tips & Tricks", find the link "computer applications" and click. You will find the file there. If you do not have web access then let me know and I will e-mail it to you ><><><><><><><><><><><><><><><><><><><><><><><><><>< Scott D. Whittaker 218 Carr Hall Research Assistant Gainesville, FL 32610 University Of Florida ph 904-392-1295 ICBR EM Core Lab fax 904-846-0251 sdw@biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/ From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-JUN-1996 09:23:32.71 To: MICROARCHIVE CC: Subj: used ultra wide 10x eyepieces Message-Id: <199606041311.IAA05831@Sparc5.Microscopy.Com> X-Sender: zaluzec@microscopy.com Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Tue, 4 Jun 1996 08:15:09 -0500 To: MIcroscopy@Sparc5.Microscopy.Com From: SBDX78A@PRODIGY.COM ( KATY T RUSHNOV) (by way of zaluzec@microscopy.com (Nestor J. Zaluzec)) Subject: used ultra wide 10x eyepieces Does anyone have a pair of uw 10x eyepieces for a Nikon microscope they would like to sell? The part no. for them is 79046. My email address is sbdx78a@prodigy.com. Thank you. From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-JUN-1996 10:20:05.87 To: MICROARCHIVE CC: Subj: LM:pre-embedding fluorescent marker Mr-Received: by mta RANDD; Relayed; Tue, 04 Jun 1996 09:46:49 -0500 Mr-Received: by mta MCM$RAND; Relayed; Tue, 04 Jun 1996 09:46:52 -0500 Mr-Received: by mta RANDD; Relayed; Tue, 04 Jun 1996 09:47:14 -0500 Alternate-Recipient: prohibited Disclose-Recipients: prohibited Content-Return: prohibited Date: Tue, 04 Jun 1996 09:28:00 -0500 (CDT) From: "Jane A. Fagerland (847) 935-0104" Subject: LM:pre-embedding fluorescent marker To: microscopy@Sparc5.Microscopy.Com Message-Id: <01I5I95VTWIWAM41LY@PPDMR.Abbott.Com> Mime-Version: 1.0 Content-Type: TEXT/PLAIN; CHARSET=US-ASCII Content-Transfer-Encoding: 7BIT Posting-Date: Tue, 04 Jun 1996 09:45:00 -0500 (CDT) Importance: normal Priority: normal X400-Mts-Identifier: [;94649040606991/6386983@RAND] A1-Type: MAIL Hop-Count: 2 Some thoughts on cryosectioning lungs.....The following procedure worked very well for me when I was doing in situ hybridization in mouse lungs: > Fix the lungs intratracheally with 4% paraformaldehyde in PBS, using gravity feed > Fix at 4C overnight > Rinse in cold PBS, 3 times for 5-10 minutes each > Immerse in 30% sucrose in cold PBS, overnight at 4C or until the lungs sink > Drain excess liquid and freeze in OCT or Lipshaw M1 (I embedded them in histology molds complete with backing rings that fit in the cryostat, then froze them in liquid nitrogen in a stainless steel tray held over a styrofoam container of liquid nitrogen.) The Lipshaw medium was easier for me to section than the OCT. The morphology I got with this procedure was excellent - comparable to that in paraffin-sections. Good luck! Jane A. Fagerland, Ph.D. Dept. Microscopy and Microanalysis Abbott Laboratories Abbott Park IL 60064 From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-JUN-1996 11:24:09.66 To: MICROARCHIVE CC: Subj: Re: EDS System Selection (longish) Date: Tue, 4 Jun 1996 10:50:44 -0400 (EDT) From: "Dr. Andrew P. Somlyo" To: Oxford Instruments Pty Ltd Cc: "Paul K. Carpenter" , Microscopy@Sparc5.Microscopy.Com Subject: Re: EDS System Selection (longish) In-Reply-To: <199605240908.TAA24228@oznet02.ozemail.com.au> Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII We are attempting to replace our ISIS system. On Fri, 24 May 1996, Oxford Instruments Pty Ltd wrote: > Dear Paul, > In regard to your question, I would like to point out that the Oxford > Instruments Link ISIS system has a fully quantitative mapping package > (Quantmap). Full spectrum processing (including digital filtering and > fitting) followed by a quantitative matrix correction is performed at each > point. The resulting digital images show elemental concentrations rather > than just a scaled x-ray intensity map. Standard deviation maps are also > produced to allow significance testing. > > Your local Oxford rep would be delighted to tell you more! > Best Regards, Julie Sheffield-Parker. > > At 05:15 PM 21/05/96 -0700, you wrote: > > > > >Tell me this: who has an EDS system that can obtain a digital image of a > >sample where a full least-squares fit and ZAF correction has been done on > >the fly at each pixel, and the resulting image portrays the concentration > >of the element mapped (not a scaled x-ray intensity map). I've seen papers > >at national meetings describing this capability, as far back as 5-10 years > >ago. The limitation for sure isn't the processing speed of the computers > >now available, or the amount of RAM or hard drive storage space. How hard > >can it be to do a complete analysis at each pixel in an image? > > > >Remember, our goal was to do quantitative EDS. > > > >Paul Carpenter > > > > > > > >+----------------------------------------------------+ > >| Paul K. Carpenter paulc@gps.caltech.edu | > >| Division Analytical Facility | > >| Geological and Planetary Sciences MC 170-25 | > >| California Institute of Technology | > >| Pasadena, CA 91125 | > >| 818-395-6126 (X-ray Lab) 818-568-0935 (Dept. FAX) | > >+----------------------------------------------------+ > > > > > > > > > > > ************************************************* > From:- > > Julie Sheffield-Parker, > Oxford Instruments Pty. Ltd., > P. O. Box 7, > Pennant Hills, > NSW 2120, > Sydney, AUSTRALIA > > Tel: ++ 61 2 484 6108 > Fax: ++ 61 2 484 1667 > E-Mail: oisydney@ozemail.com.au > > ************************************************* > > From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-JUN-1996 16:49:48.08 To: MICROARCHIVE CC: Subj: EM: Flourisol X-Sender: st004718@brandywine.otago.ac.nz Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Wed, 5 Jun 1996 11:11:16 +1200 To: MICROSCOPY@Sparc5.Microscopy.Com From: richard.easingwood@stonebow.otago.ac.nz (Richard Easingwood) Subject: EM: Flourisol Has anyone heard of or/and used Flourisol? It is used fracturing samples for SEM after dehydration, the sample is frozen and fractured in this medium. I would like to know how to obtain it. Thanks in advance. Please reply to mark.gould@stonebow.otago.ac.nz Mark Gould From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-JUN-1996 17:23:06.84 To: MICROARCHIVE CC: Subj: Viton O-rings? Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Tue, 4 Jun 1996 15:20:45 -0500 To: microscopy@sparc5.microscopy.com From: bsgphy3@uconnvm.uconn.edu (JIM ROMANOW) Subject: Viton O-rings? Does anyone have a reliable source for Viton o-rings? I can not find a distibutor who will accept orders for small quantities anymore. (5 to 10 rings per part number, less than a 50-100 dollar minimum order) and many of the rings I have purchased recently seem to be of poorer quality than 10 years ago. Thank you. Jim Romanow Electron Microscopy Facility Physiology and Neurobiogy Department The University Of Connecticut Storrs bsgphy3@uconnvm.uconn.edu (860) 486-2914 voice -1936 fax From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-JUN-1996 18:20:56.34 To: MICROARCHIVE CC: Subj: Ink and Toner Analysis Date: 04 Jun 96 15:52:57 EDT From: "Steven W. Miller" <73150.2217@CompuServe.COM> To: Microscopy Fileserver Subject: Ink and Toner Analysis Message-ID: <960604195257_73150.2217_IHD130-3@CompuServe.COM> The best analysis may not be public. The FBI and IRS (yes that is the Internal Revenue Service) have extensive techniques for analysis of documents and inks. They have every kind of analytical instrument you can imagine. I would imagine most governments have similar labs. I have never tried to see how much is published but I can't imagine it is in their best interest to publish outside of forensic journals. Maybe someone else can point you to a specific forensic journal or reference. Good Luck, Steve Miller Integrated Microsystems, Inc. From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-JUN-1996 19:21:07.38 To: MICROARCHIVE CC: Subj: Absorption/Fluorescence of eosinophils. Message-Id: <31B35CB1.1FFE@oci.utoronto.ca> Date: Mon, 03 Jun 1996 14:44:17 -0700 From: "Ralph S. DaCosta" Organization: Ontario Cancer Institute-Princess Margaret Hospital X-Mailer: Mozilla 2.0 (Win16; I) Mime-Version: 1.0 To: "List: Microscopy" Cc: rdacosta@oci.utoronto.ca Subject: Absorption/Fluorescence of eosinophils. Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Dear Microscopists, I am looking for data on absorption and fluorescence spectra for human eosinophils. Perhaps one of you can tell me that they just happen to have such information sitting in a file somewhere....I hope. I have already called several flow cytometry/hematology people (ie. Coulter, etc), but unfortunately they do not have such info on hand. Curious, but true. Thanks and regards to all. Ralph S. DaCosta Department of Clinical Physics, University of Toronto, Ontario Cancer Institute, Princess Margaret Hospital, 610 University Ave., Toronto, Ontario, Canada. From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-JUN-1996 20:20:29.64 To: MICROARCHIVE CC: Subj: Cell Imaging: Phase + Fluorescence in 1 Video camera? Message-Id: <31B46ACF.5BCB@sierra.net> Date: Tue, 04 Jun 1996 16:56:47 +0000 From: "Marc C. Brande, MS, Founder (619) 587-4830 FAX: (619) 552-1516" Organization: Cultured Cell Systems (Hands-On Cultured Cell Biology/Imaging at Your Site) X-Mailer: Mozilla 2.0 (Macintosh; U; 68K) Mime-Version: 1.0 To: "List: Microscopy" Cc: confocal@ubvm.cc.buffalo.edu Subject: Cell Imaging: Phase + Fluorescence in 1 Video camera? Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Besides Optronics, are there other video cameras (digital/analog) that will give equally good cultured cell images under both Phase Contrast and Fluorescence? I know from experience that a Hamamatsu SIT that does fine with low-level fluorescence does not image comparable to a (ie.) Dage Newvicon under Phase Contrast. Is this too much to ask of a single video camera? Any comments will be greatly appreciated. Marc From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-JUN-1996 21:30:46.17 To: MICROARCHIVE CC: Subj: Unsuscribe Date: 04 Jun 96 21:53:21 EDT From: Jim Adams <75755.151@CompuServe.COM> To: list micro Subject: Unsuscribe Message-ID: <960605015321_75755.151_HHL29-1@CompuServe.COM> Please un-subscribe me from the mailing list. Thank You JJA From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-JUN-1996 22:52:43.37 To: MICROARCHIVE CC: Subj: E. Leitz microscope Message-ID: <31B564FC.22C@eastworld.net> Date: Wed, 05 Jun 1996 10:44:12 +0000 From: "Douglas St. Denny" X-Mailer: Mozilla 2.01 (Macintosh; I; 68K) MIME-Version: 1.0 To: microscopy@Sparc5.Microscopy.Com Subject: E. Leitz microscope X-URL: http://www.lars.bbsrc.ac.uk/micro/newsmail.html Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Can anyone help me find the manufacturing date of an E. Leitz stereo microscope, serial number 399230. It has three sets of eye pieces, and three nose pieces. I would also be interested in finding a collectors group, and knowing of any good books about collecting microscopes. Is there a "Price Guide" that would let me know the market value or if this microscope is even worthwhile as a collectible? Thank you, Douglas St. Denny, Discovery Bay, Hong Kong e mail - saint@eastworld.net From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-JUN-1996 22:52:59.61 To: MICROARCHIVE CC: Subj: Re: LM: pre-embedded fluorescent marker Date: Tue, 4 Jun 1996 16:06:23 -0400 (EDT) From: Rachel Teitelbaum To: tom moninger cc: Steve Barlow , microscopy@sparc5.microscopy.com Subject: Re: LM: pre-embedded fluorescent marker In-Reply-To: Message-ID: MIME-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII > > Freshly prepared formaldehyde should not give you any autofluorescence or > quench the probe to any great extent. I have had good luck fixing probes > such as various fluo-dextrans, lectins, some membrane dyes, etc. > > Lung is often a pain to cryo-section. I'd start with the easiest, a PF fixed > piece of tissue, freeze it in OCT and section. > This may still interfere with the label though. A neat trick with lung is to heat the oct in a syringe (5 cc syringe with a 26g needle) at 37 degrees, in an incubator, and while warm, inject into the trachea, inflating the lung that way. Sectioning will be much better than if immersed only in oct. You can then freeze, cryosection, label, and then fix with acetone, and lose no antigenicity. Hope this helps. -Rachel From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-JUN-1996 23:20:36.22 To: MICROARCHIVE CC: Subj: Uranyl Acetate Saftey Data Date: Tue, 4 Jun 1996 18:36:26 -0500 Message-Id: <2.2.16.19960605092314.2427e27e@geel.dwt.csiro.au> X-Sender: vei011@geel.dwt.csiro.au X-Mailer: Windows Eudora Pro Version 2.2 (16) Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" To: Microscopy@Sparc5.Microscopy.Com, austem@rsbs-central.anu.edu.au From: Colin Veitch Subject: Uranyl Acetate Saftey Data Hi all, We are about to start using uranyl acetate for some staining applications but have no MSDS information regarding the handling or disposal of it. We can only get information for uranium products in general. The company which produced it no longer produces it and the company they suggested may have information no longer exists! A search of the WWW has also drawn a blank! If anyone out there could email this information to me it would be greatly appreciated as it is required with some urgency! Many thanks in advance. Colin Veitch ##################################################################### # # # Colin.Veitch@geel.dwt.csiro.au # # Instrumentation Scientist # # CSIRO Division of Wool Technology Tel. +61 (0) 52 275611 # # P.O. Box 21 Fax. +61 (0) 52 275657 # # BELMONT Vic 3216 # # Australia # # # # "We see the Universe the way it is because if it were different, # # we would not be here to observe it." # # # ##################################################################### From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 5-JUN-1996 05:24:49.66 To: MICROARCHIVE CC: Subj: Micro Lumina Date: Wed, 5 Jun 1996 11:24:10 +0200 (MET DST) Message-Id: <199606050924.LAA22558@glen.ib.be> X-Sender: jlec6732@mail.ib.be X-Mailer: Windows Eudora Version 1.4.4 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" To: microscopy@Sparc5.Microscopy.Com From: orion@infoboard.be (Jean Leclef) Subject: Micro Lumina Hello all, the Micro Lumina camera can be found at the following addresses (this is of course not a limited list): - in the US: contact ElectroImage NY at - electroimg@aol.com - microbill@aol.com - fax 516 773 2955 - tel 516 773 4305 - in Belgium: contact E.L.I. sprl at - orion@infoboard.be - fax (32) 67 22 09 53 - tel (32) 67 21 25 07 - in Germany - 100116.1420@compuserve.com - Leica Vertrieb GmbH (fax (49) 6251-136-185) - in France - I.C.I. sarl Belfort (fax (33) 84 54 03 98 - tel (33) 84 58 02 43) good luck - hope this will help you John orion@infoboard.be From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 5-JUN-1996 07:20:04.15 To: MICROARCHIVE CC: Subj: CCD camera Message-Id: <199606051148.AA14756@admd04.hs.sll.se> Date: Wed, 5 Jun 1996 12:56:07 +0100 To: microscopy@Sparc5.Microscopy.Com From: BICH@PATD01.HS.SLL.SE (Birger Christensson) Subject: CCD camera We are in the process of setting up a system for fluoresent microscopy, wide field, for deconvolution and 3-D analysis of signaltransduction molecules. I would very much appreciate suggestions in the choice of: camera (is Sensys good enough? or alternatives to Photometrics), filter/shutter wheel system, Z-focus drive (accuracy/stability), and deblurring/deconvolution algorithms. Are there any evalations/comparisons of these components published or otherways available. All help much aprreciated Birger ------------------------------------------------------------------------- Birger Christensson, MD, PhD Dept. of Pathology, F49 Huddinge University Hospital, S-14186 Huddinge, SWEDEN, Tel +46-8-7461000 Fax +46-8-7795520 bich@PATD01.HS.SLL.SE From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 5-JUN-1996 08:24:41.20 To: MICROARCHIVE CC: Subj: Re: Uranyl Acetate Saftey Da Message-Id: Date: 5 Jun 1996 07:43:26 -0400 From: "Linda Iadarola" Subject: Re: Uranyl Acetate Saftey Da To: austem@rsbs-central.anu.edu.au, "Colin Veitch" , "Microscopy@Sparc5.Microscopy.Co" X-Mailer: Mail*Link SMTP-QM 3.0.3 b1 d5 RE>Uranyl Acetate Saftey Data 6/5/96 Dear Colin, I have recently purchased uranyl acetate from Polysciences, Inc. USA. Along with my order I was given an MSDS sheet. I hope it is helpful, but please keep in mind, these follow United States safety standards as well as universal precautions. Cheers, Linda Iadarola. Section I Identification: uranyl acetate (uranium oxyacetate) C4H6O6U.2H2O Section II Hazardous Ingredients: depleted uranium at (0.00028mCi/g) Section III Physical Data: Boiling point 527 (decomposes); Solubility in water; appearance, yellow and solid; Melting Point 110C. None of the following apply:vapor pressure, vapor density, % volatile by volume, evaporation rate, specific gravity. Section IV Explosion and fire hazard data: extinguishing media: water, carbon dioxide, dry chemical powder, foam. Special fire fighting procedures: firefighters must wear self-contained breathing aparatus and fully protective equipment. No unusual fire and explosion hazards. Section V Health hazards: Routes of entry: inhalation, skin and ingestion. Extremely toxic by inhalation and ingestion. Danger of cumulative health effects, may result in kidney damage. Emergency and First Aid procedures: skin contact-wash affected area with copious amounts of water. eye contact-fluch eyes with water for at least 15 minutes. inhalation-remove to fresh air. give oxygen or artificial respiration as needed. ingestion-wash out mouth thoroughly and induce vomiting. call physician. Section VI Reactivity Data: stable, avoid heat, incompatible with oxidizing agents, hazardous decomposition products include carbon monoxide and coarbon dioxide Section VII Spill or Leak Procedures: Steps to take if spills or leaks occur-wear self-contained breathing aparatus, rubber boots and gloves. Sweep up. Do not raise dust. Wash and ventilate spill site after pick up is complete. Waste disposal method-bury in approved landfill according to federal, state and local regulations. Section VIII Special protection information: Use respiratory protection, ventilate with local exhaust, wear rubber gloves and safety goggles, keep shower and eye bath in local area. Section IX Special precautions: Store material at room temperature. Keep storage container tightly closed. Avoid exposure of food or drink to product. Cover any cuts or skin abrasions. Wash thoroughly after handling. -------------------------------------- Date: 6/5/96 1:19 AM To: Linda Iadarola From: Colin Veitch Hi all, We are about to start using uranyl acetate for some staining applications but have no MSDS information regarding the handling or disposal of it. We can only get information for uranium products in general. The company which produced it no longer produces it and the company they suggested may have information no longer exists! A search of the WWW has also drawn a blank! If anyone out there could email this information to me it would be greatly appreciated as it is required with some urgency! Many thanks in advance. Colin Veitch ##################################################################### # # # Colin.Veitch@geel.dwt.csiro.au # # Instrumentation Scientist # # CSIRO Division of Wool Technology Tel. +61 (0) 52 275611 # # P.O. Box 21 Fax. +61 (0) 52 275657 # # BELMONT Vic 3216 # # Australia # # # # "We see the Universe the way it is because if it were different, # # we would not be here to observe it." # # # ##################################################################### ------------------ RFC822 Header Follows ------------------ Received: by QuickMail.Yale.edu with SMTP;5 Jun 1996 01:14:07 -0400 Received: (from daemon@localhost) by Sparc5.Microscopy.Com (8.6.11/8.6.11) id SAA06754 for dist-Microscopy; Tue, 4 Jun 1996 18:36:29 -0500 Received: from GEEL.DWT.CSIRO.AU (fred.geel.dwt.csiro.au [192.203.235.254]) by Sparc5.Microscopy.Com (8.6.11/8.6.11) with SMTP id SAA06751 for ; Tue, 4 Jun 1996 18:36:26 -0500 Date: Tue, 4 Jun 1996 18:36:26 -0500 Received: from VEITCH ([192.203.235.170]) by GEEL.DWT.CSIRO.AU with SMTP; Wed, 5 Jun 1996 9:21:07 +1000 (EST) Message-Id: <2.2.16.19960605092314.2427e27e@geel.dwt.csiro.au> X-Sender: vei011@geel.dwt.csiro.au X-Mailer: Windows Eudora Pro Version 2.2 (16) Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" To: Microscopy@Sparc5.Microscopy.Com, austem@rsbs-central.anu.edu.au From: Colin Veitch Subject: Uranyl Acetate Saftey Data From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 5-JUN-1996 11:21:03.94 To: MICROARCHIVE CC: Subj: unsubscribe Date: Wed, 5 Jun 1996 08:21:58 -0700 (PDT) From: David S Leaf To: "List: Microscopy" Subject: unsubscribe Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII please unsubscribe From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 5-JUN-1996 13:07:39.74 To: MICROARCHIVE CC: Subj: SUBSCRIBE Message-ID: <7294B53101F70300@mhs.unc.edu> Date: Wed, 5 Jun 1996 11:26:25 -0400 From: "Schoonhoven, Robert" Sender: "Schoonhoven, Robert" Organization: UNC To: microscopy@Sparc5.Microscopy.Com Subject: SUBSCRIBE X-Mailer: Connect2-SMTP 4.00.b27E MHS to SMTP Gateway SUBSCRIBE From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 5-JUN-1996 13:52:41.85 To: MICROARCHIVE CC: Subj: uranyl acetate From: "NANCY SMITH" Organization: School of Science CSU Hayward To: microscopy@Sparc5.Microscopy.Com Date: Wed, 5 Jun 1996 09:23:03 PSD8PDT Subject: uranyl acetate Priority: normal X-Mailer: Pegasus Mail/Windows (v1.22) Message-Id: <7A2BFD1EA3@darwin.sci.csuhayward.edu> Colin Veitch requested information regarding uranyl acetate handling and safety data. Could respondents please post their replies to the list? The responses would be valuable to many of us. Thank you Nancy Crise Smith From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 5-JUN-1996 15:02:20.22 To: MICROARCHIVE CC: Subj: Cheap Scopes Date: 05 Jun 96 14:08:32 EDT From: James Thomas <73772.3542@CompuServe.COM> To: Marcia Miller Subject: Cheap Scopes Message-ID: <960605180832_73772.3542_FHC64-1@CompuServe.COM> Marcia, Thanks for the info. We received it via our Cray XMP or maybe it was a PCXT. RMM From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 5-JUN-1996 15:50:07.30 To: MICROARCHIVE CC: Subj: EPMA : standards for Rb and Cs Message-Id: <199606051826.OAA17138@julian.uwo.ca> To: microscopy Subject: EPMA : standards for Rb and Cs Date: Wed, 05 Jun 96 14:26:02 -0500 From: Yves Thibault X-Mailer: E-Mail Connection v2.5.03 -- [ From: Yves Thibault * EMC.Ver #2.5.02 ] -- Hi to all, I am planning to analyze with an elctron probe microanalyzer a series of synthetic alkali-rich silicate-phosphate glasses. Some will be made with Cs and others with Rb. I do not have proper standards for these two elements (Cs,Rb). I was wondering if someone would know a good source for such standards. Thank you, Yves Thibault Dept of Earth Sciences University of Western Ontario London, Ontario, CANADA, N6A 5L9 e-mail ythibaul@julian.uwo.ca From: SMTP%"rinman@calshp.cals.wisc.edu" 5-JUN-1996 17:15:47.73 To: MICROARCHIVE CC: Subj: unsubscribe Message-Id: <199606051830.AA287989419@calshp.cals.wisc.edu> From: "Ross Inman" To: "microscopy@sparc5.microscopy.com" Date: Wed, 05 Jun 96 13:27:27 Reply-To: "Ross Inman" Priority: Normal X-Mailer: Ross B. Inman's Registered PMMail 1.5 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Content-Transfer-Encoding: 7bit Subject: unsubscribe unsubscribe Ross B. Inman Institute for Molecular Virology University of Wisconsin-Madison 1525 Linden Drive Madison, Wisconsin 53706 USA Phone: 608 262- 9881 FAX: 608 262-7414 E-mail: rinman@calshp.cals.wisc.edu WWWeb: http://phage.bocklabs.wisc.edu/ FTP: phage.bocklabs.wisc.edu (anonymous OK) Talk: rinman@phage.bocklabs.wisc.edu From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 5-JUN-1996 17:18:18.11 To: MICROARCHIVE CC: Subj: TEMs for sale From: Kurt.Albertine@hsc.utah.edu Date: Wed, 05 Jun 1996 09:42 -0700 (MST) Subject: TEMs for sale To: Microscopy@Sparc5.Microscopy.Com Message-id: <31B5AAC6.6D0E.4B1C.000@in2> MIME-version: 1.0 Content-type: TEXT/PLAIN Content-transfer-encoding: 7BIT Dear Nestor Zaluazac: I am writing to you through Steve Kuzmic and Jonathan Krupp. Jonathan provided some information about the MSA bulletin board service. Would you please post the following message for me? For sale: Two (2) JEOL 100S transmission electron microscopes: $5,000 each. Both are in excellent condition; one is in mint condition. Spare parts from a third JEOL 100S are also available at no additional cost with purchase of one of the microscopes. Relocation fee of each instrument will be $2,000 (Steve Kuzmic; S & J Services). Contact Kurt H. Albertine, Ph.D., University of Utah, at eMail address: Kurt.Albertine@hsc.utah.edu (FAX 801-585-7395). Thank you. Kurt H. Albertine, Ph.D. Director, Health Sciences Center Research Microscopy Facility . From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 5-JUN-1996 17:50:23.35 To: MICROARCHIVE CC: Subj: EPMA: TN-5500 replacement ? Message-Id: Date: 05 Jun 1996 10:26:10 CST From: "Liang, Long" Subject: EPMA: TN-5500 replacement ? To: microscopy@Sparc5.Microscopy.Com Comment: MEMO Dear Microscopists, I have a JEOL 733 electron probe in my lab. The operations of the probe are controlled by using TN-5500, TN-5600, and Task-5 WDS automation program (written in Flextran language). The TN-5500 is an old system which is costy for maintenance. Does anyone know of any compatible (or better) PC system which can replace the TN-5500 ? Thanks in advance. Long Liang ARCO EPMA/SEM Lab Plano, TX lliang@is.arco.com From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 5-JUN-1996 18:19:49.17 To: MICROARCHIVE CC: Subj: job posting Message-ID: <31B633AA.3FB0@sover.net> Date: Wed, 05 Jun 1996 18:26:02 -0700 From: McPherson Family X-Mailer: Mozilla 2.0 (Win16; I) MIME-Version: 1.0 To: microscopy@Sparc5.Microscopy.Com CC: gangof4@sover.net Subject: job posting X-URL: http://www.lars.bbsrc.ac.uk/micro/newsmail.html Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Omega Optical is looking for a qualified individual to fill the position of Product Manager for fluorescence microscopy products. Specifically we want someone who has strengths in micrscopy (hardware, software, optics, chemistry, life sciences) combined with strong communication skills (verbal and written). If anyone knows of someone who would qualify and has an interest in such a position, they can submit CV or resume to Human Resources via e-mail or other methods. John McPherson omega@sover.net Omega Optical Box 573 3 Grove Street Brattleboro, VT 05301 From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 5-JUN-1996 19:00:31.21 To: MICROARCHIVE CC: Subj: Re: Viton O-rings? From: William Tivol Message-Id: <199606051700.NAA17923@wadsworth.ph.albany.edu> Subject: Re: Viton O-rings? To: bsgphy3@uconnvm.uconn.edu (JIM ROMANOW) Date: Wed, 5 Jun 1996 13:00:43 -0400 (EDT) Cc: microscopy@Sparc5.Microscopy.Com In-Reply-To: from "JIM ROMANOW" at Jun 4, 96 03:20:45 pm X-Mailer: ELM [version 2.4 PL23] Content-Type: text Content-Length: 773 > > Does anyone have a reliable source for Viton o-rings? I can not find a > distibutor who will accept orders for small quantities anymore. (5 to 10 > rings per part number, less than a 50-100 dollar minimum order) and many of > the rings I have purchased recently seem to be of poorer quality than 10 > years ago. Dear Jim, Our last order (for 5-10 of each of 4 sizes, and totaling > $100) was from Web Seal Inc., 206 Marcellus St., Syracuse NY 13204, phone # (315) 475-8496. I believe they also carry other elastomers, such as polypropylene, which is the most radiation-resistant of the usual materials. The quality seems to be the same as in the past, judging from how they are when we remove them from the column for our annual cleaning. Yours, Bill Tivol From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 5-JUN-1996 19:19:41.87 To: MICROARCHIVE CC: Subj: RE-Viton O-ring Source Message-ID: Date: 5 Jun 1996 14:26:44 -0400 From: "Wil Bigelow" Subject: RE-Viton O-ring Source To: "Micros/O-rings" X-Mailer: Mail*Link SMTP/QM 3.0.0GM Subject: Time: 2:15 PM OFFICE MEMO RE:Viton O-ring Source Date: 6/5/96 The Zatkoff Company in Detroit Michigan will sell O-rings in small lots, and they stock O-rings in a very large range of sizes and materials. I just picked up an assortment of Viton and Teflon O-rings from them yesterday. Their addresss is: Zatkoff Seals 23230 Industrial Park Drive Farmington Hills, MI 48335-2850 Ph: 810-478-2400 Fx:810-478-3392 Their offices are mainly in the midwest; however, I am sure that if you were to call in an order they would be willing to ship to any part of the country. Good luck, W. C. Bigelow (bigelow@umich.edu) From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 5-JUN-1996 19:42:35.73 To: MICROARCHIVE CC: Subj: Re: E. Leitz microscope Date: Wed, 5 Jun 1996 11:13:46 -0400 (EDT) From: Elinor Solit Subject: Re: E. Leitz microscope To: "Douglas St. Denny" Cc: microscopy@Sparc5.Microscopy.Com In-Reply-To: <31B564FC.22C@eastworld.net> Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Hi Douglas, The person who can help you to date the Leitz microscope may be Jan Hinsch. He works in the Leica New Jersey branch. The number there is 201-767-1100, and the Fax is 201-767-4196. If you'd like to write him, the address is 24 Link Drive, Rockleigh, NJ 07647. Jan may also know of the collectors group or the market source that you seek. Good luck to you, and my regards to Mr. Hinsch. Elinor Solit The Microscope Book On Wed, 5 Jun 1996, Douglas St. Denny wrote: > Can anyone help me find the manufacturing date of an E. Leitz stereo > microscope, serial number 399230. It has three sets of eye pieces, and > three nose pieces. I would also be interested in finding a collectors > group, and knowing of any good books about collecting microscopes. Is > there a "Price Guide" that would let me know the market value or if > this microscope is even worthwhile as a collectible? > > Thank you, > Douglas St. Denny, > Discovery Bay, Hong Kong > > e mail - saint@eastworld.net > From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 5-JUN-1996 20:19:42.33 To: MICROARCHIVE CC: Subj: Oil Immersion Message-ID: Date: 5 Jun 1996 18:01:24 -0500 From: "Fermin, Cesar" Subject: Oil Immersion To: "EM Users (MSA)" , "NIHImageUsers" X-Mailer: Mail*Link SMTP-MS 3.0.2 I am sudenly having reaction to two different oil immersion we have. Please send source for supplier of different types of oil immersion of high quality. Is the Cargille Lab still in business? Send response to me at address below. Gracias. ********************************************************************* *Cesar D. Fermin, Ph.D \|T|/ Fax (504) 587-7389 * *Tulane Medical School /|M|\ Voice Mail (504) 584-2618 * *Pathology/SL79 \|C|/ Secretary (504) 584-2436 * *New Orleans La 70112-2699 /|*|\ Lab/Techn. (504) 584-2521 * * ---->Prof. Pathology & Otolaryngology * *http://www1.omi.tulane.edu/departments/pathology/fermin/cdftop.html* ********************************************************************* From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 5-JUN-1996 20:22:48.00 To: MICROARCHIVE CC: Subj: Re: Uranyl Acetate Saftey Data X-Sender: wight@mailserver.nist.gov Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Wed, 5 Jun 1996 15:49:31 -0500 To: Microscopy@sparc5.microscopy.com From: scott.wight@nist.gov (Scott Wight) Subject: Re: Uranyl Acetate Saftey Data Cc: Colin Veitch Colin and fellow netters: The best place to get information of this type is on the SAFETY listserver, it has as many safety professionals as this list has microscopists. Directions to send a message and/or join the list are below: ------------------------------------------------------------------------------ TO SEND E-MAIL MESSAGES TO THE LIST: ========================================= Send your messages to SAFETY@UVMVM.UVM.EDU Be sure to use a clear Subject header so other people can follow discussion threads easily, and the digest index accurately reflects the contents. Note that when you "Reply" to someone, that reply usually goes to the whole list, not just that person. LISTSERV COMMANDS ======================= FOR ALL COMMANDS LISTED BELOW: Send them to LISTSERV@UVMVM.UVM.EDU. DO NOT send these to SAFETY or your message will be sent to all subscribers, and will have NO effect on your options! Put the commands in the body of the e-mail message, not in the title. To SUBscribe === To add your name to the list, send a message saying SUB SAFETY (the brackets are not necessary). To UNSUBscribe ===== To unsubscribe from SAFETY send the command UNSUB SAFETY to LISTSERV ----------------------------------------------------------------------------- >Hi all, > >We are about to start using uranyl acetate for some staining applications >but have no MSDS information regarding the handling or disposal of it. We >can only get information for uranium products in general. The company which >produced it no longer produces it and the company they suggested may have >information no longer exists! A search of the WWW has also drawn a blank! > >If anyone out there could email this information to me it would be greatly >appreciated as it is required with some urgency! > >Many thanks in advance. > >Colin Veitch > >##################################################################### ># # ># Colin.Veitch@geel.dwt.csiro.au # ># Instrumentation Scientist # ># CSIRO Division of Wool Technology Tel. +61 (0) 52 275611 # ># P.O. Box 21 Fax. +61 (0) 52 275657 # ># BELMONT Vic 3216 # ># Australia # ># # ># "We see the Universe the way it is because if it were different, # ># we would not be here to observe it." # ># # >##################################################################### Scott Wight e-mail: SCOTT.WIGHT@NIST.GOV NIST - Microanalysis Group W voice: 301-975-3949 Bld 222, Rm A113 | fax: 301-216-1134 or 301-417-1321 Gaithersburg, MD 20899 \|/ disclaimer: Any opinion expressed is my own and does not represent those of my employer. From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 5-JUN-1996 21:19:28.28 To: MICROARCHIVE CC: Subj: Uranyl Acetate Safety Data Date: Thu, 6 Jun 1996 09:43:19 +1000 Message-Id: <199606052343.JAA28692@ultra.ultra.net.au> X-Sender: pns@mailhost.ultra.net.au (Unverified) X-Mailer: Windows Eudora Light Version 1.5.2 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" To: Microscopy@Sparc5.Microscopy.Com, austem@rsbs-central.anu.edu.au From: Probing & Structure Subject: Uranyl Acetate Safety Data you wrote: Hi all, We are about to start using uranyl acetate for some staining applications but have no MSDS information regarding the handling or disposal of it. We can only get information for uranium products in general. The company which produced it no longer produces it and the company they suggested may have information no longer exists! A search of the WWW has also drawn a blank! If anyone out there could email this information to me it would be greatly appreciated as it is required with some urgency! Many thanks in advance. Colin Veitch ##################################################################### # # # Colin.Veitch@geel.dwt.csiro.au # # Instrumentation Scientist # # CSIRO Division of Wool Technology Tel. +61 (0) 52 275611 # # P.O. Box 21 Fax. +61 (0) 52 275657 # # BELMONT Vic 3216 # # Australia # # # # "We see the Universe the way it is because if it were different, # # we would not be here to observe it." # # # ##################################################################### Colin and whoever is looking for those Material Safety Data Sheets: A great many of these are available at these two sites University of Utah gopher://gopher.chem.utah.edu:70/11/MSDS North West Fisheries http://research.nwfsc.noaa.gov/msds.html In Australia some "wise guy" decided to require the facts to be set out in a different order. They do though allow the European Community MSDS but the American MSDS form is not acceptable here. Since several hundred thousand chemicals are imported this is a big job and hugely expensive. We have converted most of the EM chemical MSDS and they are available at our site. Regards Jim Darley Probing & Structure Microscopy Supplies & Accessories Phone +61 77 740 370 Fax: +61 77 892 313 Internet Catalogue: http://www.ultra.net/~pns/ From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 5-JUN-1996 22:23:00.35 To: MICROARCHIVE CC: Subj: Thanks for Uranyl Acetate infromation Date: Wed, 5 Jun 1996 21:19:17 -0500 Message-Id: <2.2.16.19960606115605.3a4731b0@geel.dwt.csiro.au> X-Sender: vei011@geel.dwt.csiro.au X-Mailer: Windows Eudora Pro Version 2.2 (16) Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" To: austem@rsbs-central.anu.edu.au, Microscopy@Sparc5.Microscopy.Com From: Colin Veitch Subject: Thanks for Uranyl Acetate infromation Hi all, Just a note to say thanks for all the replies regarding my question about Uranyl Acetate. It seems that the issue of disposal is not yet resolved but some clues were gleaned from the responses. Some of the MSDS's were faxed but all the email responses have been compiled into the one (large) file. Rather than send this to the reflector, if you would like a copy of the file (in ASCII text or Word 6 for Windows - please specify) I'll email a copy to you! Thanks again, Colin Veitch ##################################################################### # # # Colin.Veitch@geel.dwt.csiro.au # # Instrumentation Scientist # # CSIRO Division of Wool Technology Tel. +61 (0) 52 275611 # # P.O. Box 21 Fax. +61 (0) 52 275657 # # BELMONT Vic 3216 # # Australia # # # # "We see the Universe the way it is because if it were different, # # we would not be here to observe it." # # # ##################################################################### From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 5-JUN-1996 23:09:09.47 To: MICROARCHIVE CC: Subj: EM/EDX: finding thin hydrocarbon coatings Message-Id: X-Sender: tania@pop.mindlink.net X-Mailer: Windows Eudora Version 1.4.4 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Wed, 05 Jun 1996 13:13:12 -0700 To: Microscopy@Sparc5.Microscopy.Com From: tania@dynamotive.com (Tania Jones) Subject: EM/EDX: finding thin hydrocarbon coatings hello, our lab is trying to find a way to detect very thin (<1um thick) coatings of stearates on the surface of a galvanized wire. our equipment consists of only a sem and a standard edx (no light element). any suggestions on how to "see" the coating would be very helpful. thank you in advance, tania jones laboratory manager dynamotive technologies corp. From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-JUN-1996 10:13:16.05 To: MICROARCHIVE CC: Subj: teaching videos Date: Thu, 06 Jun 1996 10:40:03 +0100 From: deborah Lietz Subject: teaching videos To: microscopy@sparc5.microscopy.com Cc: dlietz@trentu.ca Message-id: Content-type: TEXT/plain; charset=US-ASCII Content-transfer-encoding: 7BIT Does anyone know of any videos which specifically address block trimming and sectioning or any other microscopy video catalog other than the one put out by the MSA? From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-JUN-1996 11:14:08.67 To: MICROARCHIVE CC: Subj: teaching videos Date: Thu, 06 Jun 1996 10:39:11 +0100 From: deborah Lietz Subject: teaching videos To: microscopy@Sparc5.Microscopy.Com Cc: dlietz@trentu.ca Message-id: Content-type: TEXT/plain; charset=US-ASCII Content-transfer-encoding: 7BIT Does anyone know of any videos which specifically address block trimming and sectioning or any other microscopy video catalog other than the one put out by the MSA? From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-JUN-1996 12:12:21.92 To: MICROARCHIVE CC: Subj: TEM em-markers, Message-Id: X-Mailer: Novell GroupWise 4.1 Date: Thu, 06 Jun 1996 11:23:16 -0400 From: John Gabrovsek To: Microscopy@Sparc5.Microscopy.Com Subject: TEM em-markers, I would like to thank to all who responded to my question:"How to visualize injected plastic microspheres which migrate through the arterial wall in TEM preparation?" I got many different suggestions. Very interesting. Thanks again. Listserver is great communication medium. Regards, John Gabrovsek CCF Cleveland, Ohio From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-JUN-1996 13:12:41.31 To: MICROARCHIVE CC: Subj: RE- Cargille Labs Message-ID: Date: 6 Jun 1996 12:55:23 -0400 From: "Wil Bigelow" Subject: RE- Cargille Labs To: "Micros/Cargille" X-Mailer: Mail*Link SMTP/QM 3.0.0GM Subject: Time: 12:48 PM OFFICE MEMO RE: Cargille Labs Date: 6/6/96 According to the latest directory of manufacturers published by R & D Magazine the address for Cargille Laboratories is 55 Commerce Rd., Cedar Grove, NJ 07009; Ph: 201-239-6633; Fx: 201-239-6096 Good lick, Wil Bigelow (Bigelow@umich.edu) From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-JUN-1996 14:09:38.53 To: MICROARCHIVE CC: Subj: SEM-HISTO SECTIONS From: KLEABONAS.MATTHEW_P+@ALBANY.VA.GOV Subject: SEM-HISTO SECTIONS Date: 06 Jun 96 10:51 EDT Message-Id: <4262507@ALBANY.VA.GOV> To: MICROSCOPY@Sparc5.Microscopy.Com HI MICRO-FANS, I AM HAVING A BIT OF A PROBLEM SECURING WAX SECTIONS TO CARBON STUBS. AFTER DEPARIFFINIZATIN AND CONDUCTIVE COATING THE SECTIONS HAVE A TENDENCY TO LIFT OR CURL. IF ANY ONE HAS HAD ANY EXPERIENCE WITH PARRAFIN SECTIONS FOR SEM THE INFO WOULD BE GREATLY APPRECIATED THANKS, MATT KLEABONAS STRATTON VA MEDICAL CENTER ALBANY,NY TEL: (518)-462-3311 X2552 FAX: (518)-462-1258 E-MAIL: KLEABONAS.MATTHEW_P+@ALBANY.VA.GOV From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-JUN-1996 15:14:36.63 To: MICROARCHIVE CC: Subj: Re: EM/EDX: finding thin hydrocarbon coatings Date: Thu, 6 Jun 1996 12:34:22 -0400 (EDT) From: Elinor Solit Subject: Re: EM/EDX: finding thin hydrocarbon coatings To: Tania Jones Cc: Microscopy@Sparc5.Microscopy.Com In-Reply-To: Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Tania, Try cross-sectioning the material. If you have a cryostat all the better. If you have difficulty, it will probably come from a need to support the sample. Then it becomes a case of trying to find the best material. Sometimes a fairly stiff plastic can help. Just be sure you know what layer you're looking at in the microscope. Good luck, Elinor Solit The Microscope Book On Wed, 5 Jun 1996, Tania Jones wrote: > hello, > > our lab is trying to find a way to detect very thin (<1um thick) coatings of > stearates on the surface of a galvanized wire. our equipment consists of only > a sem and a standard edx (no light element). any suggestions on how to "see" > the coating would be very helpful. > > thank you in advance, > > tania jones > laboratory manager > dynamotive technologies corp. > From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-JUN-1996 15:24:54.04 To: MICROARCHIVE CC: Subj: Re: EM/EDX: finding thin hydrocarbon coatings Date: Thu, 6 Jun 1996 10:04:57 -0400 (EDT) From: Joe D Geller Subject: Re: EM/EDX: finding thin hydrocarbon coatings To: Microscopy In-Reply-To: Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII On Wed, 5 Jun 1996, Tania Jones wrote: > hello, > > our lab is trying to find a way to detect very thin (<1um thick) coatings of > stearates on the surface of a galvanized wire. our equipment consists of only > a sem and a standard edx (no light element). any suggestions on how to "see" > the coating would be very helpful. > > thank you in advance, > > tania jones > laboratory manager > dynamotive technologies corp. > I would be concerned about electron stimulated desorption of the stearate coating on the wire. A good way to analyze this would be to use ESCA (XPS) Electron Spectroscopy for Chemical Analysis (X-ray Photoelectron Spectroscopy). This non-ionizing technique uses x-rays for excitation and detects the emitted photoelectrons. By analyzing the electron binding energy one can determine the near (5nm) surface composition of the wire. Joe Geller Geller Microanalytical Lab jg@gellermicro.com From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-JUN-1996 15:55:26.79 To: MICROARCHIVE CC: Subj: Re: EPMA : standards for Rb and Cs Date: Thu, 6 Jun 1996 09:54:21 -0400 (EDT) From: Joe D Geller Subject: Re: EPMA : standards for Rb and Cs To: Microscopy In-Reply-To: <199606051826.OAA17138@julian.uwo.ca> Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII On Wed, 5 Jun 1996, Yves Thibault wrote: > -- [ From: Yves Thibault * EMC.Ver #2.5.02 ] -- > > Hi to all, > > I am planning to analyze with an elctron probe microanalyzer a series of > synthetic alkali-rich silicate-phosphate glasses. Some will be made with Cs > and others with Rb. I do not have proper standards for these two elements > (Cs,Rb). I was wondering if someone would know a good source for such > standards. > > Thank you, > > Yves Thibault > Dept of Earth Sciences > University of Western Ontario > London, Ontario, CANADA, N6A 5L9 > > e-mail ythibaul@julian.uwo.ca > We have a CsI standard and can prepare (as a custom standard) RbI. These compounds are stable and behave well under electron beam irradiation. Considering the accuracy of current ZAF programs, such as CITZAF, these should be satisfactory standards for analyzing oxides of Cs and Rb. Please note that we offer standards for EPMA as a normal part of our business. Joe Geller Geller MicroAnalytical Laboratory jg@gellermicro.com 508 887-7000 From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-JUN-1996 16:11:30.95 To: MICROARCHIVE CC: Subj: Got it-Oil-immer Message-ID: Date: 6 Jun 1996 15:55:32 -0500 From: "Fermin, Cesar" Subject: Got it-Oil-immer To: "EM Users (MSA)" , "NIHImageUsers" X-Mailer: Mail*Link SMTP-MS 3.0.2 Thanks for the prompt response. I now know that Cargille is still in business and dozen of suppliers who sell their oil. I still do not know if there are out there non allergenic immersion oils. Meanwhile I will just have to use glove with oil immersion. From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-JUN-1996 17:11:58.74 To: MICROARCHIVE CC: Subj: Electron Flight Simulator From: DChernoff@aol.com Date: Thu, 6 Jun 1996 17:06:11 -0400 Message-ID: <960606170610_408749889@emout19.mail.aol.com> To: microscopy@sparc5.microscopy.com Subject: Electron Flight Simulator Keith, I was sent a reply to your initial request from Veronique Buschmann. I wanted to correct an error in the e-mail address and the web site. The correct e-mail address is: dchernoff@aol.com The correct web site is: http://members.aol.com/smworld100/index.htm Please contact me if you have any questions about the Electron Flight Simulator program. Best Regards Don Chernoff Small World From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-JUN-1996 17:17:37.05 To: MICROARCHIVE CC: Subj: Re: EPMA: TN-5500 replacement ? Date: Thu, 6 Jun 1996 09:59:34 -0400 (EDT) From: Joe D Geller Subject: Re: EPMA: TN-5500 replacement ? To: Microscopy In-Reply-To: Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII On 5 Jun 1996, Liang, Long wrote: > Dear Microscopists, > > I have a JEOL 733 electron probe in my lab. The operations of the probe > are controlled by using TN-5500, TN-5600, and Task-5 WDS automation > program (written in Flextran language). The TN-5500 is an old system > which is costy for maintenance. > > Does anyone know of any compatible (or better) PC system which can > replace the TN-5500 ? > > Thanks in advance. > > Long Liang > ARCO EPMA/SEM Lab > Plano, TX > lliang@is.arco.com > > We manufacture a replacement system for the TN-5500, 5600 and TASK WDS automation, including the energy dispersive x-ray analyzer. For the EDS we supply replacement HV and bias supply units that is not Nim Bin and a PC based pulse height analyzer with qualitative and quantitative software integrated with the WDS. Part of the package includes optional digital imaging. We do have demonstraton disks available. Joe Geller jg@gellermicro.com Geller MicroAnalytical Lab 426e Boston St. Topsfield, MA 01983 508 887-7000, fax 508 887-6671 From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-JUN-1996 17:46:16.46 To: MICROARCHIVE CC: Subj: video equipment Date: Thu, 06 Jun 1996 10:18:00 +0100 From: deborah Lietz Subject: video equipment To: microscopy@sparc5.microscopy.com Cc: dlietz@trentu.ca Message-id: Content-type: TEXT/plain; charset=US-ASCII Content-transfer-encoding: 7BIT We are in the midst of purchasing some digital equipment for our microscopy lab. If you are a regular to the forum you may remember being asked for some suggestions. Well now we are getting to the task of sorting through all the files we have gathered. I'm looking for opinions of those of you working with equipment in the field who have no vested interest in any specific companies. Please give positive and negative drawbacks and try to address the following questions: 1. Does anyone have equipment hooked up to a cambridge S90 and if so what do you have? 2. Are you currently using semicaps (Genie or 1000)?and give opinion of system 3. Other than semicaps is there any other company that uses active beam control (ie controls electron beam)to maximize resolution? 4. Is your system user friendly and geered for multiple users? 5. Is your system Mac or not? Just some background information: We have an electron microscopy class of 24 students and we are trying to expose them to the art of digital archiving as well as aleviate some time spent in the darkroom. We also in our biology department have 10 computer stations which are on our network.(Macs). The system is going to be used for capturing images from the sem,lm and copystand , and tem. These images will be labeled etc. and down loaded through the network for tutorials on the computers. Images will be supported in colour and can also be used for manuals and manuscripts. Thanks, sincerely Debbie Lietz Electron Microscopy Suite Department of Biology Trent University Peterborough, Ontario K9J 7B8 email DLietz@trentu.ca From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-JUN-1996 18:10:13.45 To: MICROARCHIVE CC: Subj: Electronic booking etc Date: Thu, 6 Jun 1996 16:11:08 -0500 From: ldm2@apollo.numis.nwu.edu (L.D.Marks) Message-Id: <199606062111.AA02786@apollo.numis.nwu.edu> To: microscopy@Sparc5.Microscopy.Com Subject: Electronic booking etc This thread may be a little old, but we have had a system for this for many years and it is way better than paper. Ours is not commercial, and I quake at giving it to anyone else (it works, but it is a mess). I found out today that a system which has been installed on a couple of other microscopes here is being sold commercialy, at a rather low (I think) price. If you are interested, contact Richard Benassi at rbenassi@mcs.com . Before you ask, I have not commercial interest in this, neither does Northwestern University. From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-JUN-1996 18:42:07.84 To: MICROARCHIVE CC: Subj: video equipment for microscopy Date: Thu, 06 Jun 1996 10:20:52 +0100 From: deborah Lietz Subject: video equipment for microscopy To: microscopy@Sparc5.Microscopy.Com Cc: dlietz@trentu.ca Message-id: Content-type: TEXT/plain; charset=US-ASCII Content-transfer-encoding: 7BIT We are in the midst of purchasing some digital equipment for our microscopy lab. If you are a regular to the forum you may remember being asked for some suggestions. Well now we are getting to the task of sorting through all the files we have gathered. I'm looking for opinions of those of you working with equipment in the field who have no vested interest in any specific companies. Please give positive and negative drawbacks and try to address the following questions: 1. Does anyone have equipment hooked up to a cambridge S90 and if so what do you have? 2. Are you currently using semicaps (Genie or 1000)?and give opinion of system 3. Other than semicaps is there any other company that uses active beam control (ie controls electron beam)to maximize resolution? 4. Is your system user friendly and geered for multiple users? 5. Is your system Mac or not? Just some background information: We have an electron microscopy class of 24 students and we are trying to expose them to the art of digital archiving as well as aleviate some time spent in the darkroom. We also in our biology department have 10 computer stations which are on our network.(Macs). The system is going to be used for capturing images from the sem,lm and copystand , and tem. These images will be labeled etc. and down loaded through the network for tutorials on the computers. Images will be supported in colour and can also be used for manuals and manuscripts. Thanks, sincerely Debbie Lietz Electron Microscopy Suite Department of Biology Trent University Peterborough, Ontario K9J 7B8 email DLietz@trentu.ca From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-JUN-1996 19:08:23.07 To: MICROARCHIVE CC: Subj: Electropolishing Mg?? Date: Thu, 6 Jun 1996 09:43:26 -0400 (EDT) From: Ian Hall To: microscopy@Sparc5.Microscopy.Com Subject: Electropolishing Mg?? Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Colleagues Working temporarily far from my books and reference manuals, I need to make thin foils of a magnesium alloy. Any helpful suggestions from experienced TEM thin foil preparers on the following points would be very welcome indeed. Electrolyte: Voltage: Temperature Special handling, foil rinsing, storage etc... (Material: Mg-9Al-1Zn, die cast. Available equipment: Fischione twin-jet.) Thanks in anticipation for any help. Rick Hall Materials Science, Univ. of Delaware (presently at: Technion, Israel Institute of Technology) From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-JUN-1996 19:09:00.66 To: MICROARCHIVE CC: Subj: Re: SEM-HISTO SECTIONS Message-Id: <199606062112.AA24231@ux1.cso.uiuc.edu> Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Thu, 6 Jun 1996 16:16:16 -0500 To: microscopy@Sparc5.Microscopy.Com From: oshel@ux1.cso.uiuc.edu (philip oshel) Subject: Re: SEM-HISTO SECTIONS >HI MICRO-FANS, >I AM HAVING A BIT OF A PROBLEM SECURING WAX SECTIONS TO CARBON >STUBS. AFTER DEPARIFFINIZATIN AND CONDUCTIVE COATING THE SECTIONS >HAVE A TENDENCY TO LIFT OR CURL. IF ANY ONE HAS HAD ANY EXPERIENCE >WITH PARRAFIN SECTIONS FOR SEM THE INFO WOULD BE GREATLY APPRECIATED >THANKS, >MATT KLEABONAS Try poly-l-lysine or chromating the stubs--chrome albumin or chrome gelatin, as is done for slides. Phil &&&&&&&&&&& Illigitmi non carborundum &&&&&&&&&&& Philip Oshel soon-to-be-closed Center for Electron Microscopy University of Illinois Rm 74 Bevier Hall 905 S. Goodwin Ave. Urbana, IL 61801 (217) 244-3145 oshel@ux1.cso.uiuc.edu *********** looking for a job again *********** From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-JUN-1996 19:15:31.35 To: MICROARCHIVE CC: Subj: Re: SEM HISTOSECTIONS Date: Thu, 6 Jun 1996 14:46:17 -0400 (EDT) From: rutledge phil X-Sender: prutle1@umbc8.umbc.edu To: KLEABONAS.MATTHEW_P+@ALBANY.VA.GOV cc: microscopy@Sparc5.Microscopy.Com Subject: Re: SEM HISTOSECTIONS In-Reply-To: <199606061322.IAA01858@Sparc5.Microscopy.Com> Message-ID: MIME-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII On Thu, 6 Jun 1996 KLEABONAS.MATTHEW_P+@ALBANY.VA.GOV wrote: > HI MICRO-FANS, > ATTEMPTS TO SECURE WAX SECTIONS FOR SEM-EDXA HAVE BEEN SOMEWHAT > UNSUCCESFUL. AFTER CONDUCTIVE COATING SECTIONS CURL AND SOME- > TIMES PARTLY DETACH. TECH INFO WOULD BE GREATLY APPRECIATED. > MATT KLEABONAS > STRATTON VAMC > ALBANY,NY > TEL: (518)-462-3311 X2552 > E-MAIL KLEABONAS.MATTHEW_P+@ALBANY.VA.GOV > Matt: I had to do the same sort of thing on paraffin sections and I had our histologist cut the sections and place the sections on carbon planchets. I then deparaffinized the sections while they were still on the planchet. I looked at them uncoated in the SEM and did EDAX. I usd different tissues such as kidney, heart, brain, etc. I was looking for silicon particles coming from the tubing during cardio-pulmonary bypass operations. The sections seemed to adhere to the carbon planchets. Don't know if this will help or not with your applications, but you can give it a try. Peace, Phil From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-JUN-1996 19:54:06.35 To: MICROARCHIVE CC: Subj: video equipment for microscopy Date: Thu, 06 Jun 1996 10:21:05 +0100 From: deborah Lietz Subject: video equipment for microscopy To: microscopy@sparc5.microscopy.com Cc: dlietz@trentu.ca Message-id: Content-type: TEXT/plain; charset=US-ASCII Content-transfer-encoding: 7BIT We are in the midst of purchasing some digital equipment for our microscopy lab. If you are a regular to the forum you may remember being asked for some suggestions. Well now we are getting to the task of sorting through all the files we have gathered. I'm looking for opinions of those of you working with equipment in the field who have no vested interest in any specific companies. Please give positive and negative drawbacks and try to address the following questions: 1. Does anyone have equipment hooked up to a cambridge S90 and if so what do you have? 2. Are you currently using semicaps (Genie or 1000)?and give opinion of system 3. Other than semicaps is there any other company that uses active beam control (ie controls electron beam)to maximize resolution? 4. Is your system user friendly and geered for multiple users? 5. Is your system Mac or not? Just some background information: We have an electron microscopy class of 24 students and we are trying to expose them to the art of digital archiving as well as aleviate some time spent in the darkroom. We also in our biology department have 10 computer stations which are on our network.(Macs). The system is going to be used for capturing images from the sem,lm and copystand , and tem. These images will be labeled etc. and down loaded through the network for tutorials on the computers. Images will be supported in colour and can also be used for manuals and manuscripts. Thanks, sincerely Debbie Lietz Electron Microscopy Suite Department of Biology Trent University Peterborough, Ontario K9J 7B8 email DLietz@trentu.ca From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-JUN-1996 20:04:47.84 To: MICROARCHIVE CC: Subj: Re: EM/EDX: finding thin hydrocarbon coatings Alternate-Recipient: allowed Auto-Forwarded: prohibited Content-Return: allowed Disclose-Recipients: prohibited Conversion: allowed Importance: normal Priority: normal Sensitivity: Company-Confidential Subject: Re: EM/EDX: finding thin hydrocarbon coatings From: "Scott D. Walck WL/MLBT" To: tania@dynamotive.com (Tania Jones), Microscopy ListServer Message-Id: <960606130749.671@cliff.ml.wpafb.af.mil.0> Date: Thu, 6 Jun 96 13:07:49 -0400 X-Mailer: MAILworks 1.7-A-1 >hello, > >our lab is trying to find a way to detect very thin (<1um thick) coatings of >stearates on the surface of a galvanized wire. our equipment consists of only >a sem and a standard edx (no light element). any suggestions on how to "see" >the coating would be very helpful. > >thank you in advance, > >tania jones I assume that the wire is a steel since it is galvanized. It may have a ductile/brittle transition. Try this. Put a small nick in your wire (i.e. notch it), submerge it in LN2 for a while, and holding it with pliars, quickly break it. Then look at the wire in cross section to see if you have the film on the surface. If it works, it will be fast sample prep. - -Scott Walck ****************************************************************************** * Scott D. Walck, Ph.D. * * Materials Directorate Tel: (513) 255-5791 * * 2941 P St Ste 1 Fax: (513) 255-2176 * * WL/MLBT, BLDG 654 EMAIL: walcksd@ml.wpafb.af.mil * * Wright Patterson AFB, OH 45433-7750 * ****************************************************************************** From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-JUN-1996 20:05:20.85 To: MICROARCHIVE CC: Subj: Re: Oil Immersion Date: Thu, 6 Jun 1996 12:18:14 -0400 (EDT) From: Elinor Solit Subject: Re: Oil Immersion To: "Fermin, Cesar" Cc: "EM Users (MSA)" , NIHImageUsers In-Reply-To: Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Dr. Fermin, Cargille is going strong. Call them at 201-239-6633, ask for Jean Behlen or Oscar Sceen. If I can offer a non-medical opinion, you may be having an allergic reaction to the immersion oil. Allergies seem to become more frequent with passing time. Maybe the result of ever-present toxins in our environment. But as one who needs medicine to deal with a mosquito bite, I can offer you sympathy. Hope this helps. Elinor Solit, The Cambrex Group Publishers of The Microscope Book On 5 Jun 1996, Fermin, Cesar wrote: > I am sudenly having reaction to two different oil immersion we have. Please > send source for supplier of different types of oil immersion of high quality. > Is the Cargille Lab still in business? > > Send response to me at address below. Gracias. > > ********************************************************************* > *Cesar D. Fermin, Ph.D \|T|/ Fax (504) 587-7389 * > *Tulane Medical School /|M|\ Voice Mail (504) 584-2618 * > *Pathology/SL79 \|C|/ Secretary (504) 584-2436 * > *New Orleans La 70112-2699 /|*|\ Lab/Techn. (504) 584-2521 * > * ---->Prof. Pathology & Otolaryngology * > *http://www1.omi.tulane.edu/departments/pathology/fermin/cdftop.html* > ********************************************************************* > From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-JUN-1996 20:10:12.35 To: MICROARCHIVE CC: Subj: Embedding and Sectioning help Message-Id: <199606062008.PAA02814@Sparc5.Microscopy.Com> From: Ilene Sugino Subject: Embedding and Sectioning help To: Microscopy@Sparc5.Microscopy.Com Date: Thu, 6 Jun 1996 16:11:40 -0400 (EDT) X-Mailer: ELM [version 2.4 PL25] Mime-Version: 1.0 Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7bit Content-Length: 776 Medjet,Inc. located in Edison, NJ is seeking someone in a facility (hopefully closeby) who can embed rabbit cornea and section it. Embedding can be either in JB4 or Epon. They need samples prepared, sectioned and photographed ASAP. It's a total of 8 tissue pieces. Medjet will be offering monetary compensation. Please contact Dr. Peretz Feder if you can be of help at 908 635-6604 or e mail him at PFeder@aol.com. Thanks.-- ------------------------------------------------------------------------------ Ilene Sugino e-mail: suginoik@umdnj.edu UMDNJ-Ophthalmology phone: (201) 982-7746 DOC 6th Floor fax: (201) 982-7762 90 Bergen Street Newark, New Jersey 07103 ------------------------------------------------------------------------------ From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-JUN-1996 20:18:55.17 To: MICROARCHIVE CC: Subj: SEM HISTOSECTIONS Message-Id: <199606061322.IAA01858@Sparc5.Microscopy.Com> X-Sender: zaluzec@microscopy.com Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Thu, 6 Jun 1996 08:26:21 -0500 To: microscopy@Sparc5.Microscopy.Com From: KLEABONAS.MATTHEW_P+@ALBANY.VA.GOV (by way of zaluzec@microscopy.com (Nestor J. Zaluzec)) Subject: SEM HISTOSECTIONS HI MICRO-FANS, ATTEMPTS TO SECURE WAX SECTIONS FOR SEM-EDXA HAVE BEEN SOMEWHAT UNSUCCESFUL. AFTER CONDUCTIVE COATING SECTIONS CURL AND SOME- TIMES PARTLY DETACH. TECH INFO WOULD BE GREATLY APPRECIATED. MATT KLEABONAS STRATTON VAMC ALBANY,NY TEL: (518)-462-3311 X2552 E-MAIL KLEABONAS.MATTHEW_P+@ALBANY.VA.GOV From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-JUN-1996 20:25:44.01 To: MICROARCHIVE CC: Subj: stearate detection From: "Crossman, Harold" To: tania (Tania Jones) Cc: "'Microscopy'" Subject: stearate detection Date: Thu, 06 Jun 96 08:16:00 PDT Message-Id: <31B6EC14@hq_smtp> Encoding: 26 TEXT X-Mailer: Microsoft Mail V3.0 Tania, We do similar work. Mary Mager's recommendation is what I suggest as well so you don't just shoot right through the layer. I also suggest looking for metals that your eds can detect because metalworking soaps, greases etc. can contain heavier metals, e.g., aluminum. The broad classification for these lubricants is called metallic soap. Perhaps the stearate MSDS or manufacturer's literature can help as well. Another possibility is to look for impurities in the lube by looking at the lube itself. Since soap/grease making is a cheap, bulk process, you may find elements in the raw material that would help you identify it on the wire. Is there another lab in Dynamotive that can help you screen your sample so you know precisely what to look for? Also, do you have a BSED? The stuff will jump right out at you. -------------------------------------------- Harold J. Crossman OSRAM SYLVANIA INC. Lighting Research Center 71 Cherry Hill Dr. Beverly, MA 01915 Phone: (508) 750-1717 E-mail: crossman@rd.sylvania.com Our web site: www.sylvania.com From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-JUN-1996 20:28:05.16 To: MICROARCHIVE CC: Subj: uranyl acetate saftey Date: Thu, 6 Jun 1996 13:25:44 -0500 Message-Id: <199606061825.NAA17657@puccini.crl.umn.edu> From: "Gib Ahlstrand" To: Microscopy@sparc5.microscopy.com Subject: uranyl acetate saftey In response to the recent few messages on the hazards of uranium compounds used in the EM lab, I'll pass on a few quotes from the article "Potential hazards of uranium and its compounds in electron microscopy: a brief review", by James J. Darley and Hisanori Ezoe, that appeared in the Journal of Microscopy, Vol. 106, Pt 1, January 1976, pp 85-86, and save you the trouble of going to the library to dig up the article. I also wish to question the use of 'depleted' on labels. What caught my attention in this article is the stress put on the chemical toxicity of uranium; aside from its radioactivity, its also a nasty poison. Here are some quotes: 1. "The American Conference of Governmental Industrial Hygienists has set standards based on chemical toxicity. The maximum daily intake of uranium is set at under 2 micrograms. Fifty milligrams is considered a lethal dose. The maximum allowable concentration in the air is 0.5 mg for arsenic and only 0.05 mg for soluble uranium compounds per cubic metre of air. Acute poisoning is more likely with uranium compounds which are soluble in body fluids. Injury to the body is general, with the kidneys most affected..............Chronic poisoning is more likely from long term, low doses of insoluble compounds..............Insoluble compounds are more likely to lead to lung cancer. (Encyclopedia of Occupational Health and Safety, 1972)..............Chemical toxicity outweighs radiological toxicity of natural uranium." 2. "Touching, inhalation and ingestion of uranium compounds must be avoided. Particular care must be used when dealing with the powdered substances.............Evaporating uranium for shadowing should only be carried out in an evaporator vented into a fume hood." In consideration of the two points above I always work in the hood with the door pulled down a bit when removing powdered uranium compounds from their bottles for mixing solutions (same goes for sodium cacodylate which contains arsenic, and for lead salts, etc.). Of course we should not overlook the radioactive hazard posed by uranium compounds. Here are some quotes from the authors about that: 3. "Chemical toxicity outweighs radiological toxicity of natural uranium. However, it seems important to recognize that natural uranium compounds constitute a substantial source of ionizing radiation..............Natural uranium contains 99.28% U238, 0.714% U235 and 0.00548% U234................Depleted uranium contains between 0.7% and usually more that 0.3% U235. 4. "One gram of natural uranium emits 12,500 decays/s of alpha particles, 25,000/s of beta emission, and also releases some gamma radiation.....................We found the beta emission has sufficient energy to penetrate glass and blacken a photographic film after a day's exposure to a jar of uranyl acetate. The radiological toxicity of 100 g of uranyl acetate is similar to thirty vials of C14 containing 20 microcuries each, with allowance for energy and other modifying factors. The radiation hazard in a laboratory considering 100 g of a uranium compound is in the same range as the average biology laboratory using isotopes like H3, C14, P32, for tracer experiments....................it appears important for the user of uranium compounds to be aware of these facts." The authors go on to bemoan the lack of hazardous substance warnings on labels of "repacked materials from suppliers", but they wrote that 20 years ago and today most EM uranium compound labels that I have seen are generally adequately labeled as to the radioactive and toxic nature of contents. Uranyl magnesium acetate from Polysciences, Inc., carries a "poison" warning (with skull & crossbones) -but carries no warning as to the radioactivity of that compound(!). Uranyl acetate from Ted Pella, Inc., carries a 'radioactive' and 'poison' warning on its bottle, states the 0.51 microcurie activity level but does not carry the 'depleted' label. Electron Microscopy Sciences, has a radioactive warning sticker on its bottle of uranyl acetate, says it contains "U (depleted) activity 0.51 microcuries/gm/s", has no poison warning, but they do include the MSDS sheet (which also warns about carcinogenic effects of exposure) plus their own 2 page handout on the radioactive properties of UA and an explanation of units used to express radioactivity levels, including the definition of "depleted" as quoted by the authors in 3 above. The use of the word 'depleted' on uranium compound labels bothers me a little because it implies that there is NO radioactivity left in it. My American Heritage Dictionary defines 'deplete' as "to use up or exhaust, to empty." Thus 'depleted' would mean "used up, or exhausted, emptied," and clearly with respect to UA 'depleted' means only about 50% reduction in what is already a minor constituent of the uranium in UA, of U235 to "between 0.7% and usually more that 0.3%", as in quote 3 above. Even though the radioactivity level of 0.51 microcuries is stated, somehow the label 'depleted' implies that it is safer somehow, but to me there is no way to clean up uranium's image as a nasty substance. The 'depleted' label could be misleading to those not in the know about what it means here. The Electron Microscopy Sciences' handout ends with a good description of the actual situation inside that bottle: "Both natural and depleted uranium, being a mixture of isotopes and daughters, will be expected to demonstrate alpha, beta and gamma activity." That's why its labeled "radioactive', but personally I think the "depleted" label is misleading. Well, all this is to answer the original question on the dangers of uranium compounds and why it should be handled carefully like any hazardous compound, and to question the use of "depleted" on labels. The bottom line is that its 1. a toxic poison, and 2. its radioactive. Use with the usual precautions. Gib Ahlstrand, MMS Newsletter Editor Electron Optical Facility, University of Minnesota, Dept. Plant Pathology 495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249 612-625-9728 FAX, giba@puccini.crl.umn.edu "MICROSCOPY & MICROANALYSIS 96" in Minneapolis, Minnesota, Aug.11-15, 1996 From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-JUN-1996 20:31:39.27 To: MICROARCHIVE CC: Subj: Cs and Rb Date: 06 Jun 96 09:18:06 EDT From: "Charles A. Garber" <103532.3325@CompuServe.COM> To: MICROSCOPY-BB Subject: Cs and Rb Message-ID: <960606131805_103532.3325_IHL51-7@CompuServe.COM> On June 7, Yves Thibault wrote: ======================================== I am planning to analyze with an elctron probe microanalyzer a series of synthetic alkali-rich silicate-phosphate glasses. Some will be made with Cs and others with Rb. I do not have proper standards for these two elements (Cs,Rb). I was wondering if someone would know a good source for such standards. =============================================== You can find in the SPI "53 minerals mount" the mineral "pollucite". It is CsSi2AlO6. The analysis shows 30.0% Cs and 0.7%Rb as well as 1.3%Na and 0.1%K. In other words - all the alkali metals but only Cs in any reasonable amount. I doubt if it would be very much good as a standard for a major amount of Rb. The alkali metals are too reactive to mount as metals, which is the reason they are not in the SPI Supplies "44 Metals" mount. More information about these standards for microanalysis and prices can be found on our web site given below. Two other points: a) The "homogeneity" of this mineral is considered outstanding as determined both by ourselves and our customers, and b) we don't normally offer the mineral by itself, however I guess our arms could be twisted if that really was the only mineral you wanted (needed). Chuck ====================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President 1-(800)-2424-SPI SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: GVKM07A@prodigy.com West Chester, PA 19381-0656 USA Customer Service: spi2spi@2spi.com #################################### WWW: http://www.2spi.com #################################### ====================================================== From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-JUN-1996 20:43:38.39 To: MICROARCHIVE CC: Subj: Re: Electron Flight Simulator Date: Thu, 6 Jun 1996 12:42:08 +0200 (METDST) From: Veronique Buschmann Subject: Re: Electron Flight Simulator To: Keith Moulding Cc: Microscopy@Sparc5.Microscopy.Com In-Reply-To: <96Jun6.143955hkt.18937-24659+78@uxmail.ust.hk> Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII email adress for a Demo download and further information: dchernoff(..)aol.com or http://members.aol.com/smworld1000/index.htm try it! ----------------------------------------------------------------- Veronique Buschmann email: bushman@ruca.ua.ac.be EMAT phone: +32 3 218 04 95 University of Antwerp ----------------------------------------------------------------- From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-JUN-1996 20:43:44.06 To: MICROARCHIVE CC: Subj: RE: EM/EDX: finding thin hydrocarbon coatings From: "Audette, David" To: microscopy Subject: RE: EM/EDX: finding thin hydrocarbon coatings Date: Thu, 6 Jun 1996 07:39:00 -0500 Message-ID: <31B63728@msgate.corp.olin.com> Encoding: 24 TEXT X-Mailer: Microsoft Mail V3.0 Concerning tania jones request for info on: our lab is trying to find a way to detect very thin (<1um thick) coatings of stearates on the surface of a galvanized wire. our equipment consists of only a sem and a standard edx (no light element). any suggestions on how to "see" the coating would be very helpful. Tania, I would be inclined to try imaging at low accelerating voltage on your SEM first. A low <5 kev and not coating if possible can emphasize contaminants on surfaces such as the stearates. Also variations in the coating is possible to image this way but if the coating is uniform and continuous you might want to remove some to show the contrast. After that infrared (IR) spectroscopy is good for analysis of micron thick coatings. Good luck, Dave Audette Olin Research Center Cheshire, CT deaudette@corp.olin.com From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-JUN-1996 20:44:09.65 To: MICROARCHIVE CC: Subj: Electron Flight Simulator X-Sender: mcmouldk@uxmail.ust.hk X-Mailer: Windows Eudora Light Version 1.5.2 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Thu, 06 Jun 1996 14:38:26 +0800 To: Microscopy@Sparc5.Microscopy.Com From: Keith Moulding Subject: Electron Flight Simulator Message-Id: <96Jun6.143955hkt.18937-24659+78@uxmail.ust.hk> Hi, I am looking a program call Electron Flight Simulator Version 2.0 for Windows. Does anybody know where I can obtain the program from? Thanks, Keith. ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Materials Characterisation and Preparation Centre, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong. FAX: (852) 2358 2451 TEL: (852) 2358 8724 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-JUN-1996 20:44:11.10 To: MICROARCHIVE CC: Subj: Re: EM/EDX: finding thin hydrocarbon coatings X-Sender: mager@pop.unixg.ubc.ca Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Wed, 5 Jun 1996 22:38:15 -0800 To: tania@dynamotive.com (Tania Jones) From: mager@unixg.ubc.ca (Mary Mager) Subject: Re: EM/EDX: finding thin hydrocarbon coatings Cc: Microscopy@Sparc5.Microscopy.Com Dear Tania, I think the best way to see the stearates would be to drop the kV to 10 or lower. I recall seeing them as dark blobs between the steel wire and Zn coating in cross sections and I think they contained Ca. They should appear as dark, raised, smooth blobs obscuring the wire-pulling scratches before. Luck, Mary Tania wrote:>hello, > >our lab is trying to find a way to detect very thin (<1um thick) coatings of >stearates on the surface of a galvanized wire. our equipment consists of only >a sem and a standard edx (no light element). any suggestions on how to "see" >the coating would be very helpful. > >thank you in advance, > >tania jones >laboratory manager >dynamotive technologies corp. Mary Mager Electron Microscopist Metals and Materials Eng, UBC 309 - 6350 Stores Rd. Vancouver, B.C. V6T 1Z4 CANADA tel: 604-822-5648, fax: 604-822-3619 email: mager@unixg.ubc.ca From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-JUN-1996 20:44:13.63 To: MICROARCHIVE CC: Subj: TEM of Receptors X-Sender: mcmouldk@uxmail.ust.hk X-Mailer: Windows Eudora Light Version 1.5.2 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Thu, 06 Jun 1996 14:45:49 +0800 To: Microscopy@Sparc5.Microscopy.Com From: Keith Moulding Subject: TEM of Receptors Message-Id: <96Jun6.144727hkt.18922-24656+91@uxmail.ust.hk> I have ask to do TEM on a sub domain of a receptor. In particular GABA-A receptor (a protein). They are currently sitting in a Sodium Phosphate buffer with Octyl-glucoside. As this is not my field, are there any recommended ways to prepare the receptors for TEM, in particular how to stain them. Thanks in advance. Keith Moulding. ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Materials Characterisation and Preparation Centre, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong. FAX: (852) 2358 2451 TEL: (852) 2358 8724 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-JUN-1996 20:44:16.11 To: MICROARCHIVE CC: Subj: Uranyl Acetate Safety Data Date: Thu, 6 Jun 1996 14:34:50 +1000 Message-Id: <199606060434.OAA15740@ultra.ultra.net.au> X-Sender: pns@mailhost.ultra.net.au X-Mailer: Windows Eudora Light Version 1.5.2 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" To: MICROSCOPY@Sparc5.Microscopy.Com From: Probing & Structure Subject: Uranyl Acetate Safety Data >you wrote: >Hi all, > >We are about to start using uranyl acetate for some staining applications >but have no MSDS information regarding the handling or disposal of it. We >can only get information for uranium products in general. The company which >produced it no longer produces it and the company they suggested may have >information no longer exists! A search of the WWW has also drawn a blank! > >If anyone out there could email this information to me it would be greatly >appreciated as it is required with some urgency! > >Many thanks in advance. > >Colin Veitch > >##################################################################### ># # ># Colin.Veitch@geel.dwt.csiro.au # ># Instrumentation Scientist # ># CSIRO Division of Wool Technology Tel. +61 (0) 52 275611 # ># P.O. Box 21 Fax. +61 (0) 52 275657 # ># BELMONT Vic 3216 # ># Australia # ># # ># "We see the Universe the way it is because if it were different, # ># we would not be here to observe it." # ># # >##################################################################### > >Colin - and whoever is looking for those Material Safety Data Sheets: > >A great many of these are available at these two sites >University of Utah gopher://gopher.chem.utah.edu:70/11/MSDS >North West Fisheries http://research.nwfsc.noaa.gov/msds.html > >In Australia some "wise guy" decided to require that the MSDS need to be arranged differently. They do though allow the European Community MSDS, but the >American MSDS form is not acceptable here. Since several hundred thousand >chemicals are imported this is a big job and hugely expensive. We have >converted most of the EM chemical MSDS and they are available at our site. > >Regards Jim Darley >Probing & Structure >Microscopy Supplies & Accessories > >Phone +61 77 740 370 Fax: +61 77 892 313 >Internet Catalogue: http://www.ultra.net/~pns/ > > > > Probing & Structure Microscopy Supplies & Accessories Phone +61 77 740 370 Fax: +61 77 892 313 Internet Catalogue: http://www.ultra.net/~pns/ From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-JUN-1996 21:09:48.16 To: MICROARCHIVE CC: Subj: Detecting organic coating by AFM Message-ID: <199606070138.UAA20500@IndyNet.indy.net> To: "tania@dynamotive.com" , Microscopy list Subject: Detecting organic coating by AFM Date: Thu, 06 Jun 96 20:39:47 -0500 From: Don Chernoff at ASM X-Mailer: E-Mail Connection v2.5.03 Tania Jones asked about detecting a stearate coating on a galvanized wire. In addition to the techniques which have already been discussed in response to this question, I suggest the use of Tapping Mode AFM with Phase imaging. This is a powerful means of imaging spotty surfaces, that is, surfaces with spots of different composition. The web site listed below has some information on this topic ("Chemical phase imaging"). For further details, contact me offline. Don Chernoff Analytical Services Division -- Advanced Surface Microscopy, Inc. E-Mail: asm@indy.net 6009 KNYGHTON RD. Voice: 317-251-1364 INDIANAPOLIS IN 46220 Toll free: 800-374-8557 (in USA) web: http://www.a1.com/asm Fax: 317-254-8690 From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-JUN-1996 21:10:49.91 To: MICROARCHIVE CC: Subj: RE- ElectroPol Mg Message-ID: Date: 6 Jun 1996 17:10:45 -0400 From: "Wil Bigelow" Subject: RE- ElectroPol Mg To: "micros/polMg" X-Mailer: Mail*Link SMTP/QM 3.0.0GM Subject: Time: 4:38 PM OFFICE MEMO RE: ElectroPol Mg Date: 6/6/96 an old time report on polishing agents and etchants put out by one of the metallurgical company labs that is no longer in existance gives the following reagents for electrolytically polishing Magnesium: 1. 90 ml Methyl cellulose and 10 ml HCl at 10-15 V, 0.02 A/sq cm, 1-2 min; reduce to 5 V after initial polarization 2. 93 ml carbitol and 7 ml HCl @ 0.10 to 0.15 A/sq cm; passive film occurs in about 1 min - remove with dilute KOH. Continue polishing an additional 20 sec. 3. 90 ml cellosolve and 10 ml HCl at 50 to 60 V for 10 to 30 sec. 4. 70 ml acetic acid, 2 ml dist. water, 28 ml 70% perchloric acid; 20 to 30 V, 0.01 A/sq cm, for 1-2 min. 5. 80 ml ethanol, 8 ml butyl cell0solve, 16 gm sodium thiocyanate. No conditions, but stated to be Buehler reagent #10, and so it probably will work pretty well. 6. 76 ml ethanol, 14 ml water, 5 ml 70% perchloric acid; 0.6 to 0.9 A/sq cm, 60 sec., use Ni cathode 7. A chemical polish for pure Mg: conc. nitric acid; immerse in cold acid. Copious evol. of NO2 fumes subsides in about 1 min. Gives highly reflective specimen with grain boundaries revealed. Hope you find something that works satisfactorily, W. C. Bigelow (bigelow@umich.edu) From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-JUN-1996 22:12:13.87 To: MICROARCHIVE CC: Subj: Growing cells on slide chambers Message-Id: <31B734EF.43AD@sierra.net> Date: Thu, 06 Jun 1996 19:43:43 +0000 From: "Marc C. Brande, MS, Founder (619) 587-4830 FAX: (619) 552-1516" Organization: Cultured Cell Systems (Hands-On Cultured Cell Biology/Imaging at Your Site) X-Mailer: Mozilla 2.0 (Macintosh; U; 68K) Mime-Version: 1.0 To: "List: Biotechnology" Cc: cellbiol@net.bio.net, microscopy@Sparc5.Microscopy.Com, confocal@ubvm.cc.buffalo.edu Subject: Growing cells on slide chambers Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit I would most appreciate contact information for vendors of glass or plastic microscope slides with wells in which to culture cells for in situ microscopy. Thanks for your efforts in advance. Marc From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 6-JUN-1996 23:16:05.53 To: MICROARCHIVE CC: Subj: Cs and Rb Message-Id: <199606070208.WAA31258@mime2.prodigy.com> X-Mailer: Prodigy Internet GW(v0.9beta) - ae02dm02sc06 From: GVKM07A@PRODIGY.COM (DR CHARLES A GARBER) Date: Thu, 6 Jun 1996 22:08:45, -0500 To: Microscopy@Sparc5.Microscopy.Com Subject: Cs and Rb -- [ From: Charles A. Garber., Ph.d * EMC.Ver #2.10P ] -- On June 7, Yves Thibault wrote: ======================================== I am planning to analyze with an elctron probe microanalyzer a series of synthetic alkali-rich silicate-phosphate glasses. Some will be made with Cs and others with Rb. I do not have proper standards for these two elements (Cs,Rb). I was wondering if someone would know a good source for such standards. =============================================== You can find in the SPI "53 minerals mount" the mineral "pollucite". It is CsSi2AlO6. The analysis shows 30.0% Cs and 0.7%Rb as well as 1.3%Na and 0.1%K. In other words - all the alkali metals but only Cs in any reasonable amount. I doubt if it would be very much good as a standard for a major amount of Rb. The alkali metals are too reactive to mount as metals, which is the reason they are not in the SPI Supplies "44 Metals" mount. More information about these standards for microanalysis and prices can be found on our web site given below. Two other points: a) The "homogeneity" of this mineral is considered outstanding as determined both by ourselves and our customers, and b) we don't normally offer the mineral by itself, however I guess our arms could be twisted if that really was the only mineral you wanted (needed) From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 7-JUN-1996 01:08:41.63 To: MICROARCHIVE CC: Subj: reply From: DChernoff@aol.com Date: Fri, 7 Jun 1996 01:06:57 -0400 Message-ID: <960607010656_212295574@emout07.mail.aol.com> To: microscopy@Sparc5.Microscopy.Com Subject: reply Keith, I was sent a reply to your initial request from Veronique Buschmann. I wanted to correct an error in the e-mail address and the web site. The correct e-mail address is: dchernoff@aol.com The correct web site is: http://members.aol.com/smworld100/index.htm Please contact me if you have any questions about the Electron Flight Simulator program. Best Regards Don Chernoff Small World From: SMTP%"I.MacLaren@BHAM.AC.UK" 7-JUN-1996 07:11:11.56 To: MICROARCHIVE CC: Subj: RE- ElectroPol Mg To: microscopy@Sparc5.Microscopy.Com From: Ian MacLaren Organization: The University of Birmingham Date: 7 Jun 1996 12:09:20 Subject: RE- ElectroPol Mg Reply-to: I.MacLaren@BHAM.AC.UK Priority: normal X-mailer: Pegasus Mail/Mac v2.02 Message-ID: <5D2B2005B9@novell2.bham.ac.uk> When I did some electropolishing of pure Mg I used a solution of 10% perchloric acid dissolved in ethanol at a voltage of 38V, a temp of -30 degrees C and a fairly low flow rate in a Struers tenupol 3 twin jet electropolisher. I can't remember where I got this recipe, maybe it was from one of J.W. Edington's monographs on electron microscopy (unfortunately no longer in print). It seemed to give good results but I didn't do extensive work on Mg. Another paper that I have (Lay, Ayed and Nouet, Acta Met. Mat., 1992, vol 40, p 2351) describes preparation of Mg specimens using twin jet chemical polishing with 25% nitric acid in ethanol. Hope this helps _________________________________________________________________ Ian MacLaren, Telephone: 0121 414 3447 IRC in Materials, FAX: 0121 414 3441 The University of Birmingham, email: I.MacLaren@bham.ac.uk Birmingham B15 2TT, England. _________________________________________________________________ From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 7-JUN-1996 08:09:01.21 To: MICROARCHIVE CC: Subj: uranyl acetate saftey (more) X-Sender: t456b15@rds163 Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Fri, 7 Jun 1996 08:20:35 -0500 To: microscopy@Sparc5.Microscopy.Com From: fskarl@goodyear.com (Frank Karl) Subject: uranyl acetate saftey (more) To emphasize the need for caution with uranyl acetate and echo Gib Ahlstrand's comments on the toxicity I refer to "Nephron" 1996, 72(2)313-317 "Deliberate overdose of uranium: Toxicity and Treatment." An abstract can be found in CAS "Forensic Chemistry" Vol 1996, issue 12 June 10 1996. Despite these warnings uranyl acetate still remains my favorite microchemical test for sodium! These opinions are mine alone and have no relationship to my employer. Thank you. Frank Karl They that give up essential liberty to obtain a little temporary safety deserve neither liberty nor safety. Benjamin Franklin From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 7-JUN-1996 09:47:05.61 To: MICROARCHIVE CC: Subj: Heizer software? X-Sender: akracher@pop-1.iastate.edu Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Fri, 7 Jun 1996 08:51:14 -0600 To: microscopy@sparc5.microscopy.com From: akracher@iastate.edu (Alfred Kracher) Subject: Heizer software? Does a company called Heizer Software (last seen at 1941 Oak Park Blvd., Pleasant Hill, CA) still exist? The phone number I have is no longer in service. I bought some EXCEL templates years ago, and some do not work anymore with newer versions. Anyone having tips, please reply personally. Thank you! Alfred ----------------------------------------- Alfred Kracher akracher@iastate.edu http://www.public.iastate.edu/~akracher vox:515 294 5439 fax:515 294 6049 ----------------------------------------- From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 7-JUN-1996 10:34:51.21 To: MICROARCHIVE CC: Subj: Re: SEM-HISTO SECTIONS Message-Id: <199606071429.JAA15923@watson.bcm.tmc.edu> X-Sender: joiner@bcm.tmc.edu X-Mailer: Windows Eudora Version 2.1.1 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Fri, 07 Jun 1996 08:27:08 -0500 To: KLEABONAS.MATTHEW_P+@ALBANY.VA.GOV From: "Joiner Cartwright, Jr., Ph.D." Subject: Re: SEM-HISTO SECTIONS Cc: microscopy@Sparc5.Microscopy.Com At 10:51 AM 6/6/96 EDT, you wrote: >HI MICRO-FANS, >I AM HAVING A BIT OF A PROBLEM SECURING WAX SECTIONS TO CARBON >STUBS. AFTER DEPARIFFINIZATIN AND CONDUCTIVE COATING THE SECTIONS >HAVE A TENDENCY TO LIFT OR CURL. IF ANY ONE HAS HAD ANY EXPERIENCE >WITH PARRAFIN SECTIONS FOR SEM THE INFO WOULD BE GREATLY APPRECIATED >THANKS, >MATT KLEABONAS >STRATTON VA MEDICAL CENTER >ALBANY,NY >TEL: (518)-462-3311 X2552 >FAX: (518)-462-1258 >E-MAIL: KLEABONAS.MATTHEW_P+@ALBANY.VA.GOV > > ***************************** Matt - I have attached histological sections to graphite specimen holders routinely without problems. I cut the sections in the usual manner, floating them on a warm water bath. I coat the graphite holder (JEOL TEMSCAN type) with the regular histological albumin fixative (Poly Scientific Albumin Fixative "Mayer", Cat. #S110; 70 Cleveland Ave. Bay Shore, NY 11706). I pick up the floating paraffin section just like I was mounting it onto a glass slide, and let it dry. You might try warming it SLIGHTLY to improve adhesion. Then I soak it in xylene to remove the paraffin. Then I let the xylene evaporate over night. Sometimes I put the mounted specimens into the vacuum evaporator and pump it down over night to remove all traces of xylene before carbon coating them. Secondary electron imaging will give you an image that readily correlates with photographs of adjacent serial histo sections made with the light microscope. And backscatter imaging will show up exogenous mineral particles for EDS. Joiner Joiner Cartwright, Jr., Ph.D. Director, Electron Microscopy tel.: (713)798-4658 Department of Pathology, Rm.286-A FAX: (713)798-3945 Baylor College of Medicine Internet: joiner@bcm.tmc.edu One Baylor Plaza Compuserve: 71555,1206 Houston, Texas 77030 U.S.A. From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 7-JUN-1996 10:34:52.43 To: MICROARCHIVE CC: Subj: Re: SEM-HIST