From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-APR-1996 11:28:11.54 To: MICROARCHIVE CC: Subj: Re: re: safety in EM labs Date: 01 Apr 96 09:16:15 EST From: "ENERGY BEAM SCIENCES, INC" <75767.640@CompuServe.COM> To: Kenneth JT Livi Cc: Microscopy Listserver Subject: Re: re: safety in EM labs Message-ID: <960401141614_75767.640_BHQ90-5@CompuServe.COM> Dear Ken, You're right about not being able to legislate common sense. In the case of liquid nitrogen, I have seen LN2 slopping out of styrofoam cups onto peoples' clothes, into instruments, all over papers, etc. For all of the examples everyone can cite of *unnecessary* regulations, I am still amazed to see kitchen microwave ovens in labs being used to heat toxic chemicals, including fixatives and heavy metals, the fumes from which then "ventilate" into the open lab when the door is opened. In some cases, the oven is then used to heat coffee and/or lunch. Steven Slap 75767,640@compuserve.com From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-APR-1996 12:55:35.97 To: MICROARCHIVE CC: Subj: Re: Safety & Liquid N2 From: William Tivol Message-Id: <199604011602.LAA13962@elba> Subject: Re: Safety & Liquid N2 To: LPS@teknesis.demon.co.uk (Dr. L. P. Stoter) Date: Mon, 1 Apr 1996 11:02:49 -0500 (EST) Cc: microscopy@Sparc5.Microscopy.Com In-Reply-To: from "Dr. L. P. Stoter" at Mar 30, 96 07:57:02 am X-Mailer: ELM [version 2.4 PL21] Content-Type: text Content-Length: 637 > Gloves, goggles, masks (and shoes) are actualy more dangerous when handling > liquid N2 than sandles and no protection. True with the exception that the shutoff valve handle on a big LN2 tank will get cold enough to be dangerous, and does not exhibit the leidenfrost effect. Using a glove or other insulation when turning off the LN2 after filling the dewar makes good sense. > > More seriously, bureaucrats, administrators and the inexperienced should > talk to somebody who has real knowledge. > A great general rule. Reading the manual also helps, and every lab should have a safety manual on hand. Yours, Bill Tivol From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-APR-1996 13:39:09.60 To: MICROARCHIVE CC: Subj: Date: Mon, 1 Apr 1996 17:39:29 GMT Message-Id: <9604011739.AA74798@gold.uni-miskolc.hu> X-Sender: femkov@gold.uni-miskolc.hu (Unverified) X-Mailer: Windows Eudora Light Version 1.5.2 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" To: microscopy@aaem.amc.anl.gov From: Kovacs Arpad From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-APR-1996 15:10:02.73 To: MICROARCHIVE CC: Subj: Temperature controller Date: Mon, 1 Apr 1996 12:15:03 -0600 (CST) From: Weiming Yu To: microscopy@Sparc5.Microscopy.Com Subject: Temperature controller Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Dear Friends: Does anyone know which company sells temperature controller for optical microscope (specially for old immersion objective) ? Thank you. Weiming Yu, Ph.D. Dept. of Physics, UIUC 1110 W. Green Urbana, IL 61801 weiming@lfd.physics.uiuc.edu From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-APR-1996 15:42:04.65 To: MICROARCHIVE CC: Subj: HRP substrates Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Mon, 1 Apr 1996 13:16:16 -0500 To: Microscopy@Sparc5.Microscopy.Com From: tphillips@biosci.mbp.missouri.edu (Tom Phillips) Subject: HRP substrates I plan to return to some HRP-based histochemistry and thought I would stir up some controversy by asking what you all feel the best precipitating substrate for immunocytochemistry with peroxidase labels. I have always used DAB without metals. What is the disadvantage of using metal intensification? Does anybody have experience with the "stable" solution of DAB (e.g., Pierce Chemicals Metal Enhanced DAB Substrate Kit)? Are they worth the convenience? TIA. Thomas E. Phillips, Ph.D. Associate Professor of Biological Sciences Director, Molecular Cytology Core Facility 3 Tucker Hall University of Missouri Columbia, MO 65211 (314)-882-4712 (voice) (314)-882-0123 (fax) From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-APR-1996 16:57:45.29 To: MICROARCHIVE CC: Subj: Re: EM User Fees Message-Id: <199604011506.JAA12875@Sparc5.Microscopy.Com> Date: 1 Apr 1996 10:08:33 EST From: To: Subject: Re: EM User Fees To: Microscopy ListSer *** Reply to note of 04/01/96 07:19 From: Dave King (607)757-1248 T37/257-3 Ext DEKING@VNET.IBM.COM >>> Internal KING at ENDVM5 >>>> Subject: Re: EM User Fees We do outside analytical work over the complete spectrum of materials, chem lab and product assurance areas. We charge different rates for each instrument and basically different operations. Sample prep, data analysis, and report writing get charged, as well as actual SEM time, for example. We figure it as "beginning to end." Think about how much time you'd have saved if you didn't have that job at all. Everything must be covered, or someone else is subsidizing it. . < > << >> ===========> Dave King <<< >>> ------------------------------------------------------ <<<< From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-APR-1996 17:42:30.85 To: MICROARCHIVE CC: Subj: Getting data from Kevex 8000? From: mlamvik@mcnc.org X-Sender: mlamvik@robin.mcnc.org Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Mon, 1 Apr 1996 16:28:54 -0500 To: Microscopy@Sparc5.Microscopy.Com Subject: Getting data from Kevex 8000? Does anyone know how to get spectrum data out of a Kevex 8000 analyzer into a desktop computer for further analysis? Advice will be appreciated. Thanks, Michael Lamvik MCNC, Research Triangle Park, North Carolina From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-APR-1996 18:29:59.10 To: MICROARCHIVE CC: Subj: Re: microscope safety Message-Id: X-Mailer: Novell GroupWise 4.1 Date: Mon, 01 Apr 1996 11:44:05 -0800 From: Richard Thrift To: microscopy@Sparc5.Microscopy.Com Subject: Re: microscope safety I have to tell you about this: after reading the mail on this topic I used the far door to enter our microscope room. I rarely use this door. It has a sign (which I've never noticed before; it must have been there for the last half-year) saying "NOTICE Safety glasses required in this area" Richard >>> Kenneth JT Livi 03/29/96 06:39am >>> Dear Microscopists, Wil's recent comment on the safety hazards of distilled water brought to mind some peculiar safety regulations here in MD. In reference to liquid N (which can be dangerous stuff), we were first required to wear gloves while handling the stuff. Then came goggles and soon after that, full face shields. The funny thing is, the most dangerous aspect of our handling of LN2 is that students often wear sandals in the summer and are very likely to get stung by droplets. There are no safety measures for feet protection! But now that I have mentioned it, some occupational safety officer listening in will recommend new safety procedures requiring protective booties! In the end, we can't legislate common sense, nor can we abdicate responsibility to those above. (The opinions above are mine and of anyone who agrees with them.) Kenneth JT Livi Department of Earth and Planetary Sciences 34th and Charles Streets The Johns Hopkins University Baltimore, Maryland 21218 (410) 516-8342 (voice) (410) 516-7933 (fax) klivi@jhu.edu (e-mail) From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-APR-1996 19:19:44.49 To: MICROARCHIVE CC: Subj: SEM Image distortion From: "Neuberger, Damian" To: "'Microscopy '" Subject: SEM Image distortion Date: Mon, 01 Apr 96 11:46:00 PST Message-ID: <316032B0@msmail_smtp.roundlake.baxter.com> Encoding: 36 TEXT X-Mailer: Microsoft Mail V3.0 Hello All: We have a JEOL JSM-6300F microscope that exhibits a not-so-slight Y-image distortion. I hope that I can explain it clearly and that someone out there may have suggestion(s) as to the source of the problem that we have not been able to get resolved by field service personnel. Materials: (1) SEM; 2) NIST SRM 484f SEM magnification standard; 3) 2000 copper mesh grid (Pella Cat #631C) that has been calibrated as a secondary standard with the NIST standard; 4) Polaroid Type 53 film, 4x5". Conditions: Image of the mesh grid recorded at X2000, 5 keV, 25 mm WD, aperture #4 (30 micrometers), probe current setting #8 (1x10-11), camera aperture setting 5.6, scan rate 80 (sec/scan?). Micrograph is oriented so that the 5" dimension is along the X-axis. Scan direction top to bottom (Y-axis). Scan rotation is OFF, tilt correction is OFF, specimen tilt is 0, SEI mode. Problem: The micrograph as well as the viewing monitors exhibit a distortion in the Y?-axis. That is, the vertical spacing between grid bars on one end of the X-axis of the photo is about 1.5 to 2 mm greater (out of 74mm) at the right end than on the left end. This distortion is easily seen in the image of the grid. (Wouldn't a photo in this email make things a whole lot easier!) Solutions: That's where you knowledgeable microscopists come in. Any ideas will be gratefully accepted and tested! Thanks so much, everyone. Damian Neuberger neuberd@baxter.com From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-APR-1996 19:31:02.52 To: MICROARCHIVE CC: Subj: Re: EM User Fees Message-Id: <199604011514.JAA07649@mailhub.iastate.edu> X-Sender: wes@pop.ameslab.gov X-Mailer: Windows Eudora Pro Version 2.1.2 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Mon, 01 Apr 1996 09:12:22 -0600 To: rw9@psu.edu (Rosemary A. Walsh), Microscopy@Sparc5.Microscopy.Com From: Warren Straszheim Subject: Re: EM User Fees We had momentary problems with the files on a Mac. We first had to locate them, our Eudora didn't tell us which folder it dropped it in. It ended up in SYSTEM\EUDORA FOLDER\ATTACHMENTS, which we should have guessed. We could not simply double-click on it to open it. Apparently it did not have the extra information to tell the desktop what kind of file it was. Therefore, we had to first open up Word, then open the document the old-fashioned way through the FILE/OPEN menu. I have found that this is often required and usually works for documents that come through different channels. Being a Windows man myself, I am used to opening up Word and leaving it running throughout the day so it is a simple matter to switch to Word and open a new document through the pull-down menus. Sometimes the normal associations get lost or naming conventions are not followed. But the pull-dopwns almost always work. At 09:24 PM 3/31/96 +0000, you wrote: >Arthur, > Thanks for your time and effort--I had a problem opening the Mac file. > Would you please fax me the file? My FAX number is 814-863-1357. > >Rosemary Walsh >EM Facility >The Biotechnology Institute >519 Wartik Lab >The Pennsylvania State University >University Park, PA 16802 ---------------------------------------------------- Warren E. Straszheim 270 Metals Development, Ames Lab/ISU, Ames IA, 50011 Phone: 515-294-8187 FAX: 515-294-3091 E-Mail: wes@ameslab.gov (or: wesaia@iastate.edu) http://www.public.iastate.edu/~iprt_info/cfce/ (re: coal) http://www.public.iastate.edu/~wesaia/marl/ (re: SEM) coal characterization and processing electron microscopy, x-ray analysis, image analysis computer applications From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-APR-1996 20:12:37.84 To: MICROARCHIVE CC: Subj: Re: C2 movement Date: Mon, 1 Apr 1996 16:29:16 +0000 (GMT) From: Yves Maniette To: Jan van der Meijden cc: EO_SW/APB@eo.ie.philips.nl, Microscopy List Subject: Re: C2 movement In-Reply-To: Message-ID: MIME-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII To Jan van der Meijden thank you for your mail, I just checked what you recommend. The displacement is about 20 mm, so within your specifications. Despite it is within Philips specification, this does not allow to work easily from bright field-dark field and diffraction modes, because when working at about 100 000 times (usual magnification rate in order to make BF DF diff images), the movement comes to be 20*20= 400 mm, which is over my own specifications (i.e. the light goes out of the screen therefore I have to get back to 20 000 magnification in order to see where it is...). The only reasonable way I have found is to press the intensity limit softkey when being near the cross over in order to prevent from going too low in intensity of the C2 lens. This works, but I will welcome with great pleasure another method to get rid of the problem. On the other hand, during the alignment procedure, due to this shift problem, we do not know exactly how to center correcty the light. I am used to press at each step the normalization button (i.e. photo button), but am amazed because after the whole process (canon alignment) usually the light does NOT get back in the center of the screen. Well maybe it is within specifications, I haven't checked this out. Thank you for every new input, I am sure that many Philips microscope users are eager to know more about the subject. For this reason I an sending a copy to the list as well. Sincerely, Yves MANIETTE Universitat de Barcelona Serveis Cientifico Tecnics Unitat ESCA TEM Carrer Luis Sole i Sabaris E-08028 BARCELONA Tel +34 3 402 16 95 Fax +34 3 402 13 98 On Thu, 28 Mar 1996, Jan van der Meijden wrote: > Dear Sir > > Via a detour, your request to know the C2 movement when changing C2 > has arrived on my desk. > The procedure we use in the factory is as follows: > Magnification 5800x > Focus C2 and centre the beam. > Fully over focus the beam and focus again. Centre the beam again > Fully underfocus the beam and focus again. Measure the distance from > the centre. This distance should be less than 5cm. > According to your mail this distance on your scope is about 7cm at > 20.000x and thus far within specification. > The problem is caused by the high remanence in the C2 lens and the C2 > lens not perfectly mechanically aligned in the optical axis. > To overcome the problem use one spotsize higher or lower in order to > do a light normalisation. > > Regards Jan van der Meijden > > > > > Yours sincerely > > > > Jan van der Meijden > From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-APR-1996 20:19:30.97 To: MICROARCHIVE CC: Subj: EM: Marine invertebrate texts X-Sender: st004718@brandywine.otago.ac.nz Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Tue, 2 Apr 1996 13:20:10 +1100 To: MICROSCOPY@Sparc5.Microscopy.Com From: richard.easingwood@stonebow.otago.ac.nz (Richard Easingwood) Subject: EM: Marine invertebrate texts Dear Microscopists, Does anyone out there know of a textbook or any other reference material which covers integumental ultrastructure - cuticle, epithelium and associated structures and also oogenesis - in marine invertebrates. We have a marine science student who is studying subclass Ascothoracida who is having problems getting the ultrastructure info she needs. Thanks in advance. Richard Richard Easingwood South Campus Electron Microscope Unit School of Medical Sciences University of Otago PO Box 913 Dunedin NEW ZEALAND Telephone: 64-03-479 7301 Facsimile: 64-03-479 7254 SOUTHERNMOST E.M UNIT IN THE WORLD From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-APR-1996 20:48:53.00 To: MICROARCHIVE CC: Subj: Re: Microscope Incubators X-Sender: rh208@pop.cus.cam.ac.uk Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Mon, 1 Apr 1996 15:47:11 +0000 To: Brian Burgess , microscopy@Sparc5.Microscopy.Com From: rh208@cus.cam.ac.uk (Ray Hicks) Subject: Re: Microscope Incubators Hi Brian, I think that most CO2 sensors work by measuring the thermal conductivity or indirectly the heat capacity of the gas mix, this makes them awkward to use in a situation where relative humidity and temperature may fluctuate (since both of these will affect the temperature of the sensor). They also might (I don't know) need a large volume of gas to pass over them to register a change. I would suggest that you might look into three alternative strategies i) the simplest is to use a cannister of premixed gas, presuming that you want to have a constant pCO2. ii)Monitor the pH of the medium colorimetrically, assuming the colour change of the medium is mainly due to dissolved CO2, which may not be the case. iii) Measure the infra-red absorbance of the gas mix, I believe that this method is used in some incubators, but I'm not sure what wavelength to use. (i) is the easiest and cheapest assuming you don't want to vary the pCO2, if you did you could adapt it by using two bottles, one of CO2 and one of air, these methods might cause humidity regulation problems. (ii) would have to be verified, and if it worked you'd have to build your own probe (I'd have a go if you like), but you would have the benefit of regulating dissolved CO2 not just gaseous. Depending on the absorption spectrum of CO2, it might be hard to find suitable detectors and emitters for (iii). I hope this helps Ray At 2:13 pm 29/3/96, Brian Burgess wrote: >Hi Everyone >We are designing an incubator to fit on a microscope to set the >temperature at 37C and control humidity and maintain a level of >CO2 at 5%. I have had a real hard time locating a CO2 >control/sensing unit. I did find one marketed by Forma >Scientific but it is lunky and quite large. I rather need a >smaller unit. As mentioned before I need to control temperature >and humidity levels. Does anyone know of a good system or a >manufacturer of such a system? >---------------------------------------------- >Brian Burgess >Chemical Engineering >University of Florida >e-mail: burgbr@che.ufl.edu Ray Hicks ________________________________________________________________________ |University of Cambridge |Tel 01223 330149 | |Department of Medicine |Fax 01223 336846 | |Level 5, Addenbrookes Hospital |e-mail rh208@cus.cam.ac.uk | |Hills Road Cambridge |Web Page/ facsmac.med.cam.ac.uk | |CB2 |ftp server 131.111.80.78 | |UK | | |_________________________________|_____________________________________| From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-APR-1996 21:28:46.95 To: MICROARCHIVE CC: Subj: Sender: kayton@ohsu.edu (Robert Kayton) Message-Version: 2 >To: microscopy@Sparc5.Microscopy.Com (!Microscopy) From: !Microscopy-request@Sparc5.Microscopy.Com (Robert Kayton,MAC,CROET) Date: Mon Mar 18 12:38:43 -0500 1996 UA-Content-ID: Email-Version: 2 To: news@ohsu.edu To: microscopy@Sparc5.Microscopy.Com (!Microscopy) Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAI Content-Length: 1062 Title: Biological Science Technician (Plants) Lab: USDA-ARS, Salinas, CA The USDA-ARS is seeking a biological science technician (plants) (GS-404-7, 8, or 9) for the Crop Improvement and Protection Research Unit in Salinas, CA. The incumbent will share responsibilities in electron microscopy of plant virus infections for ultrastructural characteristics. The incumbent will also assist in research involving molecular, serological, and biological studies of several plant viruses infecting sugarbeet and vegetables. Candidate must have a knowledge of electron microscopy, plant virology, and knowledge of microbiological techniques. Must be a U.S. citizen. Bachelors degree is desirable. Salary is commensurate with experience ($24,610-39,140 per annum). For information regarding research program contact Gail C. Wisler or James E. Duffus (408)755-2835. For information regarding application procedures/forms contact Tom Nelson (408)755-2810. Applications must be postmarked by May 6, 1996. The USDA is an equal opportunity employer. From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-APR-1996 21:46:14.07 To: MICROARCHIVE CC: Subj: Re: LN2 Dewar Date: 01 Apr 96 09:16:50 EST From: "ENERGY BEAM SCIENCES, INC" <75767.640@CompuServe.COM> To: "Robert R.Wise" Cc: Microscopy Listserver Subject: Re: LN2 Dewar Message-ID: <960401141649_75767.640_BHQ90-8@CompuServe.COM> Bob- Energy Beam Sciences offers a range of stainless steel LN2 lab containers, from 5L up to 220L. The 10L container has a loss rate of .8 liters per day. Please contact me directly by e-mail or telephone (800-992-9037) for part numbers, prices, etc. Steven E. Slap, Vice-President 75767,640@compuserve.com From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-APR-1996 21:52:53.50 To: MICROARCHIVE CC: Subj: Re: your mail From: kna101@utdallas.edu Date: Mon, 1 Apr 1996 07:55:16 -0600 (CST) To: Kenneth JT Livi cc: microscopy@Sparc5.Microscopy.Com Subject: Re: your mail In-Reply-To: Message-ID: MIME-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Dear Kenneth: I understand your frustration with all the regulations that just keep coming for hazardous material in the work place, but I have to point out the flip side of this issue. I know of a lab that recently got into trouble because for years the regulators were leaving it up to the head of the lab to make sure precautions were taken. This lab was solely made up of students and the professor/lab head. The professor was leaving everything up to the students to do and learn, giving little if any dirrection to them. These students came from accounting and psycology and english-major backgrounds. They had no experience working in an environment full of hazardous substances, such as a histology lab. They were working with flourescent microscopy, without using the proper filters and hazardous substances without the proper protection, ie gloves, fume hoods. All waste was going down the drain! This is an EXTREME example, I know. I just wanted to show you how bad it can get if some sort of overseeing isn't in place. I've worked in histology labs for 16 years and I do get tired of having the rules changing about once a year, ie- we have a built-in book self near a fire door. It's been there 3-4 years. Last month the inspectors came through and told us to take it down because it's too close to the door. They've inspected that bookcase every year and not until now was it a problem! So I do know how you feel. Karen Pawlowski On Fri, 29 Mar 1996, Kenneth JT Livi wrote: > Dear Microscopists, > > Wil's recent comment on the safety hazards of distilled water brought to > mind some peculiar safety regulations here in MD. In reference to liquid N > (which can be dangerous stuff), we were first required to wear gloves while > handling the stuff. Then came goggles and soon after that, full face > shields. The funny thing is, the most dangerous aspect of our handling of > LN2 is that students often wear sandals in the summer and are very likely > to get stung by droplets. There are no safety measures for feet > protection! But now that I have mentioned it, some occupational safety > officer listening in will recommend new safety procedures requiring > protective booties! > In the end, we can't legislate common sense, nor can we abdicate > responsibility to those above. > > (The opinions above are mine and of anyone who agrees with them.) > > Kenneth JT Livi > Department of Earth and Planetary Sciences > 34th and Charles Streets > The Johns Hopkins University > Baltimore, Maryland 21218 > (410) 516-8342 (voice) > (410) 516-7933 (fax) > klivi@jhu.edu (e-mail) > > > From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-APR-1996 21:55:15.84 To: MICROARCHIVE CC: Subj: Re: EM User Fees Date: Mon, 1 Apr 1996 05:57:14 -0600 From: ldm2@apollo.numis.nwu.edu (L.D.Marks) Message-Id: <199604011157.AA04290@apollo.numis.nwu.edu> To: Albert_Nombres@ccm.imn.intel.com, Microscopy-request@Sparc5.Microscopy.Com, Microscopy@Sparc5.Microscopy.Com Subject: Re: EM User Fees Cc: ldm@apollo.numis.nwu.edu I have a general question about user fees, in particular how various people bill for hours. We use a "gentle" system with a computer interfaced to the microscope to track real hours, rather than a harsh system where one bills from the minute that the user walks in the door. As a consequence, we have comparatively low hours/week, and I have recently been criticized for this. I would be very interested in comments both from users and administrators about this - should TEM facilities bill like lawyers or not? From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-APR-1996 21:56:27.36 To: MICROARCHIVE CC: Subj: Re: modems From: ychen@MACC.WISC.EDU Message-Id: Date: Sun, 31 Mar 1996 23:33:15 -0600 Subject: Re: modems To: microscopy@Sparc5.Microscopy.Com X-Sender: YCHEN@vms2.macc.wisc.edu (Unverified) Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" >Date: Sun, 31 Mar 1996 11:02:41 +0000 >From: Robert.R.Wise@Sparc5.Microscopy.Com >Subject: modems >To: Microscopy@Sparc5.Microscopy.Com >X-Sender: wise@vaxa.cis.uwosh.edu >MIME-version: 1.0 >Content-type: text/plain; charset="us-ascii" >Content-transfer-encoding: 7BIT > > Although not strictly a microscopy question, the members of this >list seem to know a lot about computers as well. So here goes... > > Does anyone have any recommendations on the proper modem for >connecting a Macintosh (Centris 610 with EtherNet card) at home to a local >phone line? US Robotics has a 28.8 kbps model for about $200. What does >the 28.8 stand for? Is this just a plug in or do I have to get something >like an adapter (such as an Asante' FriendlyNet Media Adapter)? Please >help the computer illiterate. > >Bob Wise Bob, The speed of a modem is measured by Bit per Second (bps). The 28.8kbps modem will double the transmission speed than the 14.4 kbps one. The 28.8k modem uses error correction and data compression protocols called v.42bis, MNP2-5 for high speed data transmission. The installation is simple: just plug the modem cable (suppose this is a Mac modem) in the serial port marked as MODEM on your Mac, and connect phone line. You need to set up or configurate your communication software before start. EtherNet card is used for network connection, not for modem. If you have any question, please contact me. Good luck. Ya Chen Ya Chen Integrated Microscopy Resource (IMR)-- III M M RRRRRR an NIH Biomedical Research Resource I M M M M R R University of Wisconsin, Madison, WI I M M M RRRRRR 1675 Observatory Drive #167 I M M R R Madison, WI 53706 I M M R R TEL : 608-263-8481 I M M R R FAX : 608-265-4076 III M M R R Email:YChen@macc.wisc.edu IMR WWW Home Page: http://www.bocklabs.wisc.edu/imr.html 2nd Symposium on Integrated Microscopy: Sept. 20-22, 1996 From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-APR-1996 22:23:14.28 To: MICROARCHIVE CC: Subj: Re: Creutzfeld-Jakob disease Sender: kayton@ohsu.edu (Robert Kayton) Message-Version: 2 >To: Microscopy@Sparc5.Microscopy.Com From: !Microscopy-request@Sparc5.Microscopy.Com (Sverker Enestrom) Date: Tue Mar 19 09:13:29 +0100 1996 UA-Content-ID: Email-Version: 2 Subject: Re: Creutzfeld-Jakob disease Message-Id: <199603190813.AA21827@hulio.liu.se> Mime-Version: 1.0 To: "Buddy"@Sparc5.Microscopy.Com To: STEFFENS.B@calc.vet.uga.edu (!Steffens") Cc: Microscopy@Sparc5.Microscopy.Com Content-Type: text/plai Content-Length: 1367 >This information is at least 4 years old and may not be correct. >About 4 years ago (maybe a little longer) there was a lengthy review >article in either New England Journal of Medicine or JAMA regarding >the slow human neurological disorders. At that time, there were at >least 5 recognized diseases that were classified as "Alzheimer" or >"Alzheimer-like". One of these was suspected to be of prion origin, >although by now it may be classified as something else. > >One thing to consider if you are involved in studies involving human >(or any mammalian) brain tissue is that in years past, prion diseases >were commonly misdiagnosed as Alzheimers, and as humans (even >pathologists) are not perfect, this still may be the case. AD and prion diseases share the presence of amyloid precursor proteins (beta-PP, APrP) but they are distinct diseases, both starting at the synapse (beta-PP and PrP are proteins of the neuromuscular junction and CNS synapse). In some of the prion diseases prion amyloid plaques are seen together with paired helical filaments (NFT) making them "Alzheimer-like" as Budy writes. ********************************************************* Sverker Enestrom M.D., Ph.D. Department of Pathology University of Linkoping, Sweden Phone: +46 13 22 15 20 Fax: +46 13 13 22 57 ********************************************************* From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-APR-1996 23:17:12.50 To: MICROARCHIVE CC: Subj: Re: Creutzfeld-Jakob disease Sender: kayton@ohsu.edu (Robert Kayton) Message-Version: 2 >To: Microscopy@Sparc5.Microscopy.Com From: !Microscopy-request@Sparc5.Microscopy.Com (Robert Kayton,MAC,CROET) Date: Mon Mar 18 16:40:07 EST 1996 UA-Content-ID: Email-Version: 2 Subject: Re: Creutzfeld-Jakob disease Organization: College of Vet. Med To: Microscopy@Sparc5.Microscopy.Com Priority: normal X-Mailer: Pegasus Mail/Windows (v1.11a) Message-Id: <85E5ACC2A8C@calc.vet.uga.edu> Content-Type: text Content-Length: 1175 >As far as I know Alzheimers disease is not infectous---- Where did you get your >information about Alzheimers disease? This information is at least 4 years old and may not be correct. About 4 years ago (maybe a little longer) there was a lengthy review article in either New England Journal of Medicine or JAMA regarding the slow human neurological disorders. At that time, there were at least 5 recognized diseases that were classified as "Alzheimer" or "Alzheimer-like". One of these was suspected to be of prion origin, although by now it may be classified as something else. One thing to consider if you are involved in studies involving human (or any mammalian) brain tissue is that in years past, prion diseases were commonly misdiagnosed as Alzheimers, and as humans (even pathologists) are not perfect, this still may be the case. W. L. Steffens, Ph.D Dept. of Veterinary Pathology College of Veterinary Medicine University of Georgia Athens, GA 30602 STEFFENS.B@CALC.VET.UGA.EDU Voice: (706) 542-5536 FAX: (706) 542=5828 From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-APR-1996 23:42:19.60 To: MICROARCHIVE CC: Subj: Re: Creutzfeld-Jakob disease Sender: kayton@ohsu.edu (Robert Kayton) Message-Version: 2 >To: Microscopy@Sparc5.Microscopy.Com From: !Microscopy-request@Sparc5.Microscopy.Com (Robert Kayton,MAC,CROET) Date: Mon Mar 18 10:04:59 EST 1996 UA-Content-ID: Email-Version: 2 Subject: Re: Creutzfeld-Jakob disease Organization: College of Vet. Med To: Microscopy@Sparc5.Microscopy.Com Priority: normal X-Mailer: Pegasus Mail/Windows (v1.11a) Message-Id: <857C4666BE3@calc.vet.uga.edu> Content-Type: text Content-Length: 1939 >Has anyone heard of the slow viruses that can survive gluteraldehyde fixation? The infectious agents that are able to survive fixation are the prions. These agents contain protein only...no nucleic acid. Among these are Creutzfeld-Jakob, scrapie, kuru, Gersten-Straussler-Schenker, BSE (mad cow disease) and apparently some forms of Alzheimers. It is known that formaldehyde does not inactivate them, not surprising since formaldehyde is one of the least cross-linking of all fixatives, so far as proteins are concerned. My understanding is that glut. does inactivate them, but don't take my word for it. >On a similar note, there was an MSA-sponsored speaker a couple of years ago who >was encouraging EM labs to make money by offering virus identification sevices >to medical centers. The preparation protocol included advice to accept unfixed >(ie. infectious), unidentified material and prepare negative stained samples on >the bench in the laboratory. We are a CLIA and State certified human clinical laboratory, licensed for negative-stain virus identification. This has always been part of our function as a veterinary EM lab, and for about 5 years we have offered it as a service to the community. We do make a considerable amount of money from it. We accept samples only from certain reference laboratories and practitioners and these are only stool samples, mostly (>95%) from infantile diarrheas. Our submission form has an entry for any unusual precautions that must be taken (ie HIV, hepatitis, etc). Our understanding with our clients is that we don't process such samples. W. L. Steffens, Ph.D Dept. of Veterinary Pathology College of Veterinary Medicine University of Georgia Athens, GA 30602 STEFFENS.B@CALC.VET.UGA.EDU Voice: (706) 542-5536 FAX: (706) 542=5828 From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-APR-1996 00:10:50.41 To: MICROARCHIVE CC: Subj: unsubscribe From: sxm29@ems.psu.edu Message-Id: <199604020428.XAA17471@pangaea.ems.psu.edu> Date: Mon, 01 Apr 96 23:32:52 EST To: microscopy@Sparc5.Microscopy.Com Subject: unsubscribe unsubscribe From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-APR-1996 01:14:18.39 To: MICROARCHIVE CC: Subj: Printers for digital images Message-Id: <199604012310.RAA23711@watson.bcm.tmc.edu> X-Sender: joiner@bcm.tmc.edu X-Mailer: Windows Eudora Version 2.1.1 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Mon, 01 Apr 1996 17:09:46 -0600 To: microscopy@Sparc5.Microscopy.Com From: "Joiner Cartwright, Jr., Ph.D." Subject: Printers for digital images Fellow microscopists - A few months ago there was a thread on the server dealing primarily with flatbed scanners and the best technology for acquiring digital images. I may have missed out on the part of the conversation dealing with printing digital images, but perhaps one or some of you might summarize any thoughts on what technologies were considered useful. I run an EM lab in a Pathology department of a medical school. Most of my EM is diagnostic work on kidney specimens and tumors. We printed out 4,500 images for each of the two past years. I would like to go digital, but finding a printer that will handle this load cheaply and quickly, yet with the required high quality is difficult. We plan to produce the usual 3 1/4" X 4" negatives in our scope as we always have. Then we would scan the negatives into a computer (IBM clone), storing them temporarily on a large hard disk, and ultimately archiving them on a writable CD system. The prints need not be durable as once they are studied and the case closed, they would be trashed. The negatives would be archived as would be the image files on the CD's. The problem is that people are suggesting dye sub for quality and laser for quick & dirty. I need QUICK & QUALITY......and cheap. Printing on a dye sublimation printer would be too costly. There are some enhancement boards that can be added to laser printers, and I have a friend who has printed some of my scanned negatives on his Photoscan system, Philips's image recording system. They come close to answering the problem, but I am wondering what else there is out there. I believe that Philips's system may be LaserPix or PhotoJet Plus from XLI Corp. I realize that I may be asking you to repeat a conversation already carried out, but if you could pass on the consensus opinions, I would appreciate it. Joiner Cartwright, Jr., Ph.D. Director, Electron Microscopy tel.: (713)798-4658 Department of Pathology, Rm.286-A FAX: (713)798-3945 Baylor College of Medicine Internet: joiner@bcm.tmc.edu One Baylor Plaza Compuserve: 71555,1206 Houston, Texas 77030 U.S.A. From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-APR-1996 01:15:50.30 To: MICROARCHIVE CC: Subj: Safety issues in EM lab Date: Mon, 01 Apr 1996 20:23:36 EST From: GVKM07A@PRODIGY.COM (DR CHARLES A GARBER) X-Mailer: PRODIGY Services Company Internet mailer [PIM 3.2-085.43] Message-Id: <096.04949697.GVKM07A@prodigy.com> To: Microscopy@sparc5.microscopy.com Subject: Safety issues in EM lab -- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] -- I know that my own safety committee gets under my own skin once in a while. But in over twenty six years of business, let me relate by far the most serious injury that occurred in our firm. Considering that we work around high voltage, use hazardous chemicals including osmium tetroxide, deal with sometimes hazardous samples, what was our most serious injury? Would you believe it involved Polaroid film? One of the microscopists (who always claimed eye protection was not needed in an SEM lab) was opening up a case of Type 52 film. The flaps on the case, as it turned out were not creased, as they should have been, therefore the tension present in the fold, when the sealing tape on the top was slit with a razor, literally flew open, cutting the corneal surface of one of the startled technician's eyes. Fortunately sight was not lost in the eye, but there was a scar formed, and there will be the need for lifetime care under a good ophthalmologist and who knows what other impairments might show up with age. Now in retrospect, nearly twenty years later, I still feel guilty about not enforcing to an even greater degree, perhaps with the threat of being dismissed, our own "rule" that safety glasses should be worn in the laboratory. If an accident is going to happen, generally speaking it will happen when it is least expected. Now my point only is that some of these safety regulations, superfluous as some of them might sound, often times do have some basis of logic and rationale, my Polaroid film case being, I think, a good example. Chuck ====================================================== Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400 President 1-(800)-2424-SPI SPI SUPPLIES FAX: 1-(610)-436-5755 PO BOX 656 e-mail: GVKM07A@prodigy.com West Chester, PA 19381-0656 USA Customer Service: SpiSupp@aol.com ######################################################## WWW: http://mail.cccbi.chester.pa.us/spi/spihome.html ######################################################## ====================================================== From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-APR-1996 01:18:59.27 To: MICROARCHIVE CC: Subj: A little humor Sender: kayton@ohsu.edu (Robert Kayton) Message-Version: 2 >To: MICROSCOPY@Sparc5.Microscopy.Com From: !Microscopy-request@Sparc5.Microscopy.Com" " (1-914-892-2225) Date: Thu Mar 28 10:09:47 EST 96 UA-Content-ID: Email-Version: 2 Subject: A little humor Message-Id: <199603281514.JAA07617@Sparc5.Microscopy.Com> To: MICROSCOPY@Sparc5.Microscopy.Com Content-Type: text Content-Length: 2450 Sorry, I couldn't resist sharing this... It was sent to me 'as is' with no author. I'm certainly not clever enough to write this. Ron I am the Very Model of a Modern Teenage Cyberpunk* I am the very model of a modern teenage Cyberpunk I rent my own apartment and it's full of electronic junk I own a VAX, a 486, I've even got a PDP I've finished Myst and Doom but I am stumped by Wing Commander III I'm very well aquainted too with matters pornographical I have a list of image sites, both overseas and national So if you want to see a picture of that Anna Nichole Smith I'll fire up my terminal and fetch for you a naughty GIF I'm totally an anarchist, the government I'd like to wreck, Though if they were to get blown up, who'd give to me my welfare cheque? In short if you need answers that concern your electronic junk, I am the very model of a modern teenage Cyberpunk I know the ancient myths about RTM, Pengo and Mitnick I 'hack' into computers and I then perform a credit check I scare all my non-hacker friends with tales of cracker theivery and even though I'm spouting crap they'll listen and believe in me I've learned to spot a troll and I've seen flames about the way I spell, I've traced badly forged cancels and seen napalm poured on AOL I've laughed at all the newbies and their flailing cries of "You all Suck!" I've been flamed by Carasso, with an anvil I have then been struck I've hung around in alt.tasteless and seen war waged on rec.pets.cats I've spent my time in talk.bizarre and used those stupid Relay Chats In short, if you need answers that concern your electronic junk, I am the very model of a modern teenage Cyberpunk Well postings like "MAKE.MONEY.FAST", I am now somewhat wary at, I have been "Global Killfiled" by the Joel Furr Commissariat, When rosebud posts a lengthy rant 'bout Microsoft she swears is true, I know that she is just another short lived kook without a clue When I have learnt what progress has been made upon the Internet, When I know something more than just a smattering of netiquette, In short when I can have a world-wide soapbox on which I can stand I've got no time for other things, like beer and trips to Disneyland My life outside the Internet is very very sad you see I cannot get my spots to fade, my social life's a tragedy, But still if you need answers that concern your electronic junk, I am the very model of a modern teenage Cyberpunk. (With apologies to G&S) From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-APR-1996 03:11:53.66 To: MICROARCHIVE CC: Subj: Re: EM: Marine invertebrate texts X-Sender: c71956@dm.uibk.ac.at Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: quoted-printable Date: Tue, 2 Apr 1996 09:24:20 +0100 To: MICROSCOPY@Sparc5.Microscopy.Com From: dietmar.reiter@uibk.ac.at (Dietmar Reiter) Subject: Re: EM: Marine invertebrate texts >Dear Microscopists, >Does anyone out there know of a textbook or any other reference material >which covers integumental ultrastructure - cuticle, epithelium and >associated structures and also oogenesis - in marine invertebrates. We have >a marine science student who is studying subclass Ascothoracida who is >having problems getting the ultrastructure info she needs. You might browse the "Microscopic Anatomy of Invertebrates" series (Frederick W. Harrison, Burton J. Bogitsh, Eds.), Wiley-Liss, New York, 1991. It=B4s a highly comprehensive series of 15 volumes, covering in-depth ultrastructure from Protozoa up to Hemichordata, Chaetognatha, and else. -Dietmar- +++ Dietmar Reiter ++++++++++++++++ +++ Dept. of Zoology and Limnology, University of Innsbruck ++++ +++ Technikerstrasse 25, A - 6020 Innsbruck, Austria +++++++++ +++ ph: (+43)-512-507-6170 (-6161), fax: ..-507-2930 (-2957) +++ From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-APR-1996 08:15:49.49 To: MICROARCHIVE CC: Subj: unsubscribe Date: Tue, 2 Apr 1996 13:24:48 +0100 (BST) From: "M.F. Butler" To: microscopy@Sparc5.Microscopy.Com Subject: unsubscribe Message-ID: MIME-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII unsubscribe From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-APR-1996 09:15:58.19 To: MICROARCHIVE CC: Subj: Re: Printers for digital images Message-ID: <199604021254.HAA27062@IndyNet.indy.net> To: "Joiner Cartwright, Jr., Ph.D." , Microscopy list Subject: Re: Printers for digital images Date: Tue, 02 Apr 96 07:50:33 -0500 From: Don Chernoff at ASM X-Mailer: E-Mail Connection v2.5.03 We're happy with a Lexmark Optra R for our spm images. it has 1200 dpi. cost $1600 with total of 10 MB ram. don chernoff -- Advanced Surface Microscopy, Inc. E-Mail: asm@indy.net 6009 KNYGHTON RD. Voice: 317-251-1364 INDIANAPOLIS IN 46220 Toll free: 800-374-8557 (in USA) Fax: 317-254-8690 From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-APR-1996 10:27:58.99 To: MICROARCHIVE CC: Subj: RE: MSDS Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Tue, 2 Apr 1996 07:57:15 -0500 To: Microscopy@Sparc5.Microscopy.Com From: David.Rothbard@ipst.edu (David Rothbard) Subject: RE: MSDS DOT regulations DO NOT require that an MSDS accompany every shipment. DOT does have strict requirements for packaging and labeling (exterior) that are specific to the contents. DOT also requires that shippers of hazardous material have a 24 hour telephone number (their own, or a service) that can answer questions about the material. Both DOT and OSHA require training of personel that handle hazardous materials. David Rothbard -- Institute of Paper Science and Technology From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-APR-1996 11:32:48.57 To: MICROARCHIVE CC: Subj: Re: Kevex 8000 Data to PC Date: Tue, 2 Apr 1996 07:52:17 -0500 (EST) From: "James R. Stets" To: Microscopy Listserver Subject: Re: Kevex 8000 Data to PC Message-ID: MIME-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Michael Lamvik asked about moving data from a Kevex 8000 to a PC. Kevex used to sell a package to do this that cost about $1000. This bought you a cable that connected to one of your printer ports on the 8000 and your PC, a diskette with Kermit, software for the 8000, and a very thick manual. I used it many times to move images from our 8000 to a Macintosh, and it worked fine although it's not fast. I don't know if Kevex sells this anymore since the 8000 is pretty old. A gentleman named Robert Schaller was very helpful in getting us set up with it, but this was at least 5 years ago and he may or may not still be with Kevex. Jim Stets Air Products and Chemicals, Inc. Allentown, PA stetsjr@ttown.apci.com My opinions are my own, not my employer's. From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-APR-1996 12:32:48.67 To: MICROARCHIVE CC: Subj: Re: HRP substrates Message-ID: In-Reply-To: Date: Tue, 2 Apr 1996 11:23:58 -0500 From: "Schoonhoven, Robert" Sender: "Schoonhoven, Robert" Organization: UNC To: microscopy@Sparc5.Microscopy.Com Subject: Re: HRP substrates X-Mailer: Connect2-SMTP 4.00.b27E MHS to SMTP Gateway We have been using the EnVision kit sold by DAKO with great success. This kit does come with a "stable" DAB solution which works very well. Under some conditions when we need to increase the intensity we use the "Quick DAB Enhancer Solution" sold by Innovex Biosciences which works very well and will not change the color of the DAB like nickle chloride. The usual disclaimer applies to both of the companies mentioned above ie: I'm simply a satisfied customer and recieve no renueration for naming their products Robert Schoonhoven Laboratory of Molecular Carcinogenesis and Mutagenesis University of North Carolina CB#7400, Rosenau Hall Chapel Hill, NC 27599-7400 Ph. 919-966-6343 Fax 919-966-6123 From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-APR-1996 13:38:03.08 To: MICROARCHIVE CC: Subj: Re: SEM Image distortion Date: Tue, 2 Apr 1996 08:52:31 -0500 (EST) From: Joe D Geller Subject: Re: SEM Image distortion To: Microscopy In-Reply-To: <316032B0@msmail_smtp.roundlake.baxter.com> Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII If you have a digital imaging system it is possible to reduce the number of sources of trouble. Does the distortion show up on the digitally recorded image? Joe Geller Geller Microanalytical Lab 426e Boston St. Topsfield, MA 01983-1212 508 887-7000 On Mon, 1 Apr 1996, Neuberger, Damian wrote: > > Hello All: > > We have a JEOL JSM-6300F microscope that exhibits a not-so-slight Y-image > distortion. I hope that I can explain it clearly and that someone out there > may have suggestion(s) as to the source of the problem that we have not been > able to get resolved by field service personnel. > > Materials: (1) SEM; 2) NIST SRM 484f SEM magnification standard; 3) 2000 > copper mesh grid (Pella Cat #631C) that has been calibrated as a secondary > standard with the NIST standard; 4) Polaroid Type 53 film, 4x5". > > Conditions: Image of the mesh grid recorded at X2000, 5 keV, 25 mm WD, > aperture #4 (30 micrometers), probe current setting #8 (1x10-11), camera > aperture setting 5.6, scan rate 80 (sec/scan?). Micrograph is oriented so > that the 5" dimension is along the X-axis. Scan direction top to bottom > (Y-axis). Scan rotation is OFF, tilt correction is OFF, specimen tilt is 0, > SEI mode. > > Problem: The micrograph as well as the viewing monitors exhibit a > distortion in the Y?-axis. That is, the vertical spacing between grid bars > on one end of the X-axis of the photo is about 1.5 to 2 mm greater (out of > 74mm) at the right end than on the left end. This distortion is easily seen > in the image of the grid. (Wouldn't a photo in this email make things a > whole lot easier!) > > Solutions: That's where you knowledgeable microscopists come in. Any ideas > will be gratefully accepted and tested! > > Thanks so much, everyone. > > Damian Neuberger > neuberd@baxter.com > > > From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-APR-1996 14:51:05.61 To: MICROARCHIVE CC: Subj: Re: Printers for digital images Mr-Received: by mta SRVR05.MUAS; Relayed; Tue, 02 Apr 1996 08:11:53 -0400 Mr-Received: by mta SRVR05; Relayed; Tue, 02 Apr 1996 08:11:53 -0400 Mr-Received: by mta SRVR01; Relayed; Tue, 02 Apr 1996 08:13:54 -0400 Disclose-Recipients: prohibited Date: Tue, 02 Apr 1996 08:11:53 -0400 (EDT) From: "Walt Bobrowski (313) 996-7814" Subject: Re: Printers for digital images In-Reply-To: <199604012310.RAA23711@watson.bcm.tmc.edu> To: "Joiner Cartwright, Jr., Ph.D." , MSA MICROSCOPY MAILING LIST Message-Id: <3353110802041996/A12861/SRVR05/11A4120B3400*@MHS> X-Envelope-To: joiner@bcm.tmc.edu, MICROSCOPY@MSA.MICROSCOPY.COM Autoforwarded: false Mime-Version: 1.0 Content-Type: TEXT/PLAIN; CHARSET=US-ASCII Content-Transfer-Encoding: 7BIT Importance: normal Priority: normal Sensitivity: Company-Confidential Ua-Content-Id: 11A4120B3400 X400-Mts-Identifier: [;3353110802041996/A12861/SRVR05] Hop-Count: 2 Check out DOUBLE RES IV from Laser Printer Accessories Corp., a board you install into your current laserjet II or III to get 600 dpi quality. We have one and it really does make a BIG difference. 1-800-225-4098. Walter F. Bobrowski Subcellular Pathology Parke-Davis Pharmaceutical Research Ann Arbor, MI 48105 TEL: 313-996-7814 FAX: 313-996-5001 E-Mail: BOBROWW@AA.WL.COM >Fellow microscopists - > >A few months ago there was a thread on the server dealing primarily with >flatbed scanners and the best technology for acquiring digital images. I may >have missed out on the part of the conversation dealing with printing >digital images, but perhaps one or some of you might summarize any thoughts >on what technologies were considered useful. > >I run an EM lab in a Pathology department of a medical school. Most of my EM >is diagnostic work on kidney specimens and tumors. We printed out 4,500 >images for each of the two past years. I would like to go digital, but >finding a printer that will handle this load cheaply and quickly, yet with >the required high quality is difficult. We plan to produce the usual 3 1/4" >X 4" negatives in our scope as we always have. Then we would scan the >negatives into a computer (IBM clone), storing them temporarily on a large >hard disk, and ultimately archiving them on a writable CD system. The prints >need not be durable as once they are studied and the case closed, they would >be trashed. The negatives would be archived as would be the image files on >the CD's. > >The problem is that people are suggesting dye sub for quality and laser for >quick & dirty. I need QUICK & QUALITY......and cheap. Printing on a dye >sublimation printer would be too costly. There are some enhancement boards >that can be added to laser printers, and I have a friend who has printed >some of my scanned negatives on his Photoscan system, Philips's image >recording system. They come close to answering the problem, but I am >wondering what else there is out there. I believe that Philips's system may >be LaserPix or PhotoJet Plus from XLI Corp. > >I realize that I may be asking you to repeat a conversation already carried >out, but if you could pass on the consensus opinions, I would appreciate it. > > > Joiner Cartwright, Jr., Ph.D. > > Director, Electron Microscopy tel.: (713)798-4658 > Department of Pathology, Rm.286-A FAX: (713)798-3945 > Baylor College of Medicine Internet: joiner@bcm.tmc.edu > One Baylor Plaza Compuserve: 71555,1206 > Houston, Texas 77030 U.S.A. > From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-APR-1996 15:53:05.64 To: MICROARCHIVE CC: Subj: unsubscribe Date: Tue, 02 Apr 1996 13:50:44 -0500 (EST) From: James Patrick Subject: unsubscribe To: microscopy@Sparc5.Microscopy.Com Message-id: <01I32GY73KV0000N76@opus.mco.edu> X-VMS-To: IN%"microscopy@Sparc5.Microscopy.Com" MIME-version: 1.0 Content-type: TEXT/PLAIN; CHARSET=US-ASCII Content-transfer-encoding: 7BIT unsubscribe From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-APR-1996 15:53:07.21 To: MICROARCHIVE CC: Subj: unsubscribe Date: Tue, 02 Apr 1996 13:50:44 -0500 (EST) From: James Patrick Subject: unsubscribe To: microscopy@Sparc5.Microscopy.Com Message-id: <01I32GY73KV0000N76@opus.mco.edu> X-VMS-To: IN%"microscopy@Sparc5.Microscopy.Com" MIME-version: 1.0 Content-type: TEXT/PLAIN; CHARSET=US-ASCII Content-transfer-encoding: 7BIT unsubscribe From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-APR-1996 16:46:24.79 To: MICROARCHIVE CC: Subj: Re: Printers for digital images Message-Id: <199604021848.MAA01689@watson.bcm.tmc.edu> X-Sender: joiner@bcm.tmc.edu X-Mailer: Windows Eudora Version 2.1.1 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Tue, 02 Apr 1996 12:46:48 -0600 To: "Walt Bobrowski (313) 996-7814" From: "Joiner Cartwright, Jr., Ph.D." Subject: Re: Printers for digital images Cc: microscopy@Sparc5.Microscopy.Com At 08:11 AM 4/2/96 -0400, you wrote: >Check out DOUBLE RES IV from Laser Printer Accessories Corp., a board you >install into your current laserjet II or III to get 600 dpi quality. We have >one and it really does make a BIG difference. 1-800-225-4098. > >Walter F. Bobrowski >Subcellular Pathology >Parke-Davis Pharmaceutical Research >Ann Arbor, MI 48105 > >TEL: 313-996-7814 >FAX: 313-996-5001 >E-Mail: BOBROWW@AA.WL.COM **************************** Walter - Is the 600 dpi output of this system better than the 600 dpi output of the LaserJet 4 that we already have? There are boards that hot-rod the LaserJet 4 up to "2,400 equivalent dpi". One wonders if they will show "equivalent" deposits in my kidney specimens. Thank you for your reply. * * Joiner Cartwright, Jr. * * From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-APR-1996 17:40:06.82 To: MICROARCHIVE CC: Subj: Re: Printers for digital images Message-Id: <199604021847.MAA01642@watson.bcm.tmc.edu> X-Sender: joiner@bcm.tmc.edu X-Mailer: Windows Eudora Version 2.1.1 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Tue, 02 Apr 1996 12:46:38 -0600 To: slakmon@scottscientific.com (P. Slakmon) From: "Joiner Cartwright, Jr., Ph.D." Subject: Re: Printers for digital images Cc: microscopy@Sparc5.Microscopy.Com Scott - Here is a reply that may interest you. Joiner At 09:35 AM 4/2/96 -0500, you wrote: >In response to your recent message posted on the newsgroup concerning >digital printers, there are several options. The first going to laser >printers, and the second being dye sub printers. The cost of laser is >obviously cheap, but not high enough quality perhaps for publication or >reports where a photo is required. Dye sub printers ex: sony makes two >digital dye sub printers. The first being model UP-D1500CN, it ouputs a >picture size of approx. 3 5/8" x 4 3/4" which is near photographic quality. >This printer sells for approx. $ 1,850.00 US and the print cost is approx. >.86 cents. for color images. The other printer from sony is the UPD-8800 >with Interface card is approx. $7,300 US and the print cost for 8.5" x 11" >is approx. $ 2.20 These printers will ouput a print in approx. 60 seconds. >I do have sample prints if you require to see some. > >Sony makes a digital thermal printer that outputs approx. a polaroid size >image on thermal paper in about 3.5 seconds, cost per print is approx. 9 cents. > >Epson makes some low cost printers, with a decent low cost image, the only >problem with laser or ink jet with these images is that it takes quite a >while to print. > >I don't know if I was helpful or not, but if I can be of any assistance, >please feel free to contact me at your convenience. > > >Best Regards, > >Philip Slakmon >Scott Scientific > > >_______________________________________________ > SCOTT SCIENTIFIC > P.O. Box 66552 Station Cavendish, > Montreal, Quebec, H4W 3J6, Canada > > Telephone: 514-485-2309 Fax: 514-485-9931 > Voice Mail: 514-888-6509 > > WWW Site: http://www.ScottScientific.com > > E-Mail: slakmon@scottscientific.com > info@scottscientific.com > sales@scottscientific.com > admin@scottscientific.com >_________________________________________________ > > ********************************** Philip - Thank you for your reply. Dyesub, as you see, may be capable of turning out images of good enough quality to see, for example, the subtle ultrastructural changes in kidney disease, but not at a price that we can afford. And laser, although fast and cheap, cannot produce the image quality necessary to show these subtle changes. One or more of the laser enhancement boards MAY be the answer, but I am not yet convinced. * * Joiner Cartwright, Jr. * * From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-APR-1996 18:25:59.92 To: MICROARCHIVE CC: Subj: Re: unsubscribe Date: Tue, 2 Apr 1996 14:24:45 -0800 (PST) From: Len Adleman Message-Id: <199604022224.OAA12834@pollux.usc.edu> To: microscopy@Sparc5.Microscopy.Com, sxm29@ems.psu.edu Subject: Re: unsubscribe unsubscribe From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-APR-1996 19:28:57.23 To: MICROARCHIVE CC: Subj: Re: Printers for digital images Message-Id: <199604022321.RAA06357@watson.bcm.tmc.edu> X-Sender: joiner@bcm.tmc.edu X-Mailer: Windows Eudora Version 2.1.1 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Tue, 02 Apr 1996 17:20:08 -0600 To: "Buddy Steffens" From: "Joiner Cartwright, Jr., Ph.D." Subject: Re: Printers for digital images Cc: microscopy@Sparc5.Microscopy.Com At 01:51 PM 4/2/96 EST, you wrote: >I run a similar facility in the Pathology Department of a Veterinary >College, but we also serve the entire College as well as some other >parts of the university. >With our SEM, we are entirely digital now... ***************** Dr. Steffens - Thank you for your reply. The savings are one of the main reasons that I want to go digital. That includes savings in time as well as money. I am considering the LaserPix. However there are some other technologies out there that I want to look at as well. * * Joiner Cartwright, Jr. * * From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-APR-1996 20:21:41.11 To: MICROARCHIVE CC: Subj: Kevex 8000 Data to PC Message-ID: <01BB212D.85B076C0@ppp236.gol.com> From: Jeff Allbright To: "'Microscopy Listserver'" Subject: Kevex 8000 Data to PC Date: Wed, 3 Apr 1996 07:16:02 +-900 Encoding: 31 TEXT Kevex still offers the 8000 to PC communication package using Kermit. There is also a high speed data transfer option available using a SCSI interface card. This is especially useful for transferring large image files, etc. More information is available from Kevex Customer Service at +1 (415) 591-3600. - Jeff Allbright, Tokyo Michael Lamvik asked about moving data from a Kevex 8000 to a PC. Kevex used to sell a package to do this that cost about $1000. This bought you a cable that connected to one of your printer ports on the 8000 and your PC, a diskette with Kermit, software for the 8000, and a very thick manual. I used it many times to move images from our 8000 to a Macintosh, and it worked fine although it's not fast. I don't know if Kevex sells this anymore since the 8000 is pretty old. A gentleman named Robert Schaller was very helpful in getting us set up with it, but this was at least 5 years ago and he may or may not still be with Kevex. Jim Stets Air Products and Chemicals, Inc. Allentown, PA stetsjr@ttown.apci.com My opinions are my own, not my employer's. From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-APR-1996 21:23:36.07 To: MICROARCHIVE CC: Subj: Kevex 8000 Data to PC Message-ID: <01BB212E.209B9520@ppp239.gol.com> From: Jeff Allbright To: "'Microscopy Listserver'" Subject: Kevex 8000 Data to PC Date: Wed, 3 Apr 1996 07:20:33 +-900 Encoding: 33 TEXT Kevex still offers the 8000 to PC communication package using Kermit. There is also a high speed data transfer option available using a SCSI interface card. This is especially useful for transferring large image files, etc. More information is available from Kevex Customer Service at +1 (415) 591-3600. - Jeff Allbright, Tokyo Michael Lamvik asked about moving data from a Kevex 8000 to a PC. Kevex used to sell a package to do this that cost about $1000. This bought you a cable that connected to one of your printer ports on the 8000 and your PC, a diskette with Kermit, software for the 8000, and a very thick manual. I used it many times to move images from our 8000 to a Macintosh, and it worked fine although it's not fast. I don't know if Kevex sells this anymore since the 8000 is pretty old. A gentleman named Robert Schaller was very helpful in getting us set up with it, but this was at least 5 years ago and he may or may not still be with Kevex. Jim Stets Air Products and Chemicals, Inc. Allentown, PA stetsjr@ttown.apci.com My opinions are my own, not my employer's. From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-APR-1996 22:26:05.81 To: MICROARCHIVE CC: Subj: Re: Printers for digital images From: "Buddy Steffens" Organization: College of Vet. Med To: "Joiner Cartwright, Jr., Ph.D." , microscopy@Sparc5.Microscopy.Com Date: Tue, 2 Apr 1996 13:51:24 EST Subject: Re: Printers for digital images Priority: normal X-mailer: Pegasus Mail/Windows (v1.11a) Message-ID: <65E4C33BBB@calc.vet.uga.edu> > I run an EM lab in a Pathology department of a medical school. Most of my EM > is diagnostic work on kidney specimens and tumors. We printed out 4,500 > images for each of the two past years. I would like to go digital, but > finding a printer that will handle this load cheaply and quickly, yet with > the required high quality is difficult. We plan to produce the usual 3 1/4" > X 4" negatives in our scope as we always have. Then we would scan the > negatives into a computer (IBM clone), storing them temporarily on a large > hard disk, and ultimately archiving them on a writable CD system. The prints > need not be durable as once they are studied and the case closed, they would > be trashed. The negatives would be archived as would be the image files on > the CD's. > > The problem is that people are suggesting dye sub for quality and laser for > quick & dirty. I need QUICK & QUALITY......and cheap. Printing on a dye > sublimation printer would be too costly. There are some enhancement boards > that can be added to laser printers, and I have a friend who has printed > some of my scanned negatives on his Photoscan system, Philips's image > recording system. They come close to answering the problem, but I am > wondering what else there is out there. I believe that Philips's system may > be LaserPix or PhotoJet Plus from XLI Corp. > I run a similar facility in the Pathology Department of a Veterinary College, but we also serve the entire College as well as some other parts of the university. With our SEM, we are entirely digital now...I don't even support film on it. With the TEM, we have a side-mounted digital camera system (JEOL TV-CAM) that is used for image acquisition, but we still use film for publication purposes. Most of the TEM images are for study purposes only, and for this we dump the image (using the system's software) to a Laserjet 4 printer through a Laserpix board. After a bit of processing, ie BCG adjustment, unsharp mask sharpening, etc., you get a "work print" that is usually acceptable. For higher quality, we also have a Codonics dye sublimation printer, on which you can put 4 images per page. For TEM, its certainly better quality than the Laserjet, but is usually not publication quality unless you do some intensive processing. SEM and light micrographs on it however are certainly publication quality. Since most images we take will not be published, we get along fine with the Laserjet prints for TEM. We do use a high quality glossy paper in it which drives up the cost of the prints to about .12 each. That still beats the 2.00+ of the Codonics. Since going mostly digital, our TEM film images have dropped from about 2000 per year to about 200 per year. The savings in tech labor, direct costs, and chemical wastes are quite substantial. W. L. Steffens, Ph.D Dept. of Veterinary Pathology College of Veterinary Medicine University of Georgia Athens, GA 30602 STEFFENS.B@CALC.VET.UGA.EDU Voice: (706) 542-5536 FAX: (706) 542=5828 From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-APR-1996 22:27:26.97 To: MICROARCHIVE CC: Subj: Re: EM: Marine invertebrate texts Date: Tue, 2 Apr 1996 19:18:16 -0600 Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable To: microscopy@Sparc5.Microscopy.Com From: oshel@ux1.cso.uiuc.edu (Philip Oshel) Subject: Re: EM: Marine invertebrate texts >>Dear Microscopists, >>Does anyone out there know of a textbook or any other reference material >>which covers integumental ultrastructure - cuticle, epithelium and >>associated structures and also oogenesis - in marine invertebrates. We hav= e >>a marine science student who is studying subclass Ascothoracida who is >>having problems getting the ultrastructure info she needs. > >You might browse the "Microscopic Anatomy of Invertebrates" series >(Frederick W. Harrison, Burton J. Bogitsh, Eds.), Wiley-Liss, New York, >1991. It=B4s a highly comprehensive series of 15 volumes, covering in-depth >ultrastructure from Protozoa up to Hemichordata, Chaetognatha, and else. > >-Dietmar- As well as this ref, there is: Neville, A.C. 1975. _The Biology of the Arthropod Cuticle_. Vol, 4/5 in the series "Zoophysiology and Ecology". Springer-Verlag. Nothing on Ascothoracida in specific, but a good general ref, and literature review up to about 1974. Phil &&& Illigitimi non carborundum &&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&& Philip Oshel Center for Electron Microscopy University of Illinois (217)244-3145 oshel@ux1.cso.uiuc.edu From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-APR-1996 23:18:35.44 To: MICROARCHIVE CC: Subj: Printers for digital images Message-Id: <199604022321.RAA06384@watson.bcm.tmc.edu> X-Sender: joiner@bcm.tmc.edu X-Mailer: Windows Eudora Version 2.1.1 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Tue, 02 Apr 1996 17:20:17 -0600 To: microscopy@Sparc5.Microscopy.Com From: "Joiner Cartwright, Jr., Ph.D." Subject: Printers for digital images I recently posted a query concerning printers suitable for producing high quality monochrome images from micrographs scanned into a computer....a printer that would allow me to "go entirely digital" and get me out of the darkroom, at least for all imaging except the most demanding applications. I have received a number of replies. Boiled down, the answers include: 1.) For quick & dirty, but usable work prints, a laser printer producing 600+ dpi; 2.) For high quality, a dye sublimation printer; and 3.) An intermediate would be a laser printer with one of the after market enhancement boards that runs the lasers resolution up to 1,200 dpi or "2,400 dpi equivalent", whatever that means; 4.) A fourth suggestion two or three people made was the Harris PhotoPro 2000 Gray Scale Digital Printer. This is a $10,000.00 silver salt laser printer that claims only 256 dpi, but each dot has 256 gray levels available. It was stated that the cost/print was less than $1.00. Do any of you have any experience with this latter machine, and would you be willing to share your thoughts? Does the increased contrast resolution make up for the POSSIBLY low spatial resolution? Do I really want to spend ten big ones on a black & white printer? Joiner Cartwright, Jr., Ph.D. Director, Electron Microscopy tel.: (713)798-4658 Department of Pathology, Rm.286-A FAX: (713)798-3945 Baylor College of Medicine Internet: joiner@bcm.tmc.edu One Baylor Plaza Compuserve: 71555,1206 Houston, Texas 77030 U.S.A. From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 3-APR-1996 00:22:39.04 To: MICROARCHIVE CC: Subj: unsub From: stpiera@lepton.hmco.com (Aaron Stpierre) Message-Id: <199604030348.WAA18162@lepton.hmco.com> Subject: unsub To: microscopy@Sparc5.Microscopy.Com Date: Tue, 2 Apr 1996 22:48:15 -0500 (EST) X-Mailer: ELM [version 2.4 PL25] Content-Type: text unsubscribe From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 3-APR-1996 01:13:29.75 To: MICROARCHIVE CC: Subj: Re: Printers for digital images: Tests Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Tue, 2 Apr 1996 20:53:19 -0500 To: (MSA Listserver)Microscopy@Sparc5.Microscopy.Com From: Peters@BSAC.UCHC.EDU (Klaus-Ruediger Peters) Subject: Re: Printers for digital images: Tests In response to last years questions raised on the performance of digital printers I tested several printers with a 1Kx1K image. Printer performances were compared at the level of whole page images and 10x enlargements of the printed images (LaserJet, Lazar Print, Dye sub,). Results are available at http://panda.uchc.edu/htklaus/DigiLab/Printing.html In conclusion, you have to ask how many lines per inch are printed and at how many gray levels (not always the same as dots per inch), plain paper or special paper, permanence of the print, printing time, printing costs. I print 300 lpi at 256 gray levels (4,800 dpi) on plain paper in 20-45 sec for 1-10 MB of images per page at 3 cents per page with a HP LaserJet and enhancer board in archival quality. Best regards Klaus ***************************************** >At 08:11 AM 4/2/96 -0400, you wrote: > >>Check out DOUBLE RES IV from Laser Printer Accessories Corp., a board you >>install into your current laserjet II or III to get 600 dpi quality. We have >>one and it really does make a BIG difference. 1-800-225-4098. >> >>Walter F. Bobrowski >>Subcellular Pathology >>Parke-Davis Pharmaceutical Research >>Ann Arbor, MI 48105 >> >>TEL: 313-996-7814 >>FAX: 313-996-5001 >>E-Mail: BOBROWW@AA.WL.COM > >**************************** >Walter - > >Is the 600 dpi output of this system better than the 600 dpi output of the >LaserJet 4 that we already have? There are boards that hot-rod the LaserJet >4 up to "2,400 equivalent dpi". One wonders if they will show "equivalent" >deposits in my kidney specimens. Thank you for your reply. > > >* * Joiner Cartwright, Jr. * * ****************************************************************************** * : * * Klaus-Ruediger Peters, Ph.D. : WWW Home Page: * * Director, Molecular Imaging Laboratgory : * * Biomolecular Structure Analysis Center : Molecular Imaging Laboratory * * University of Connecticut Health Center : http://panda.uchc.edu/ * * 263 Farmington Ave. : htklaus/index.html * * Farmington, CT 06030-2017; U.S.A : Differential Hysteresis * * : Processing Demo at http:// * * Tel: (203) 679-3977; Fax: (203) 679-1989 : panda.uchc.edu./htbit/indiv/ * * e-mail: Peters@BSAC.UCHC.EDU : /software_docs/dhp.html * * : * **************************************************************************** ** From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 3-APR-1996 03:18:36.96 To: MICROARCHIVE CC: Subj: point counter needed Date: Wed, 3 Apr 1996 09:42:54 +0300 (EET DST) Message-Id: <199604030642.JAA23290@cc.oulu.fi> X-Sender: ota@koivu.oulu.fi X-Mailer: Windows Eudora Version 1.4.4 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" To: microscopy@Sparc5.Microscopy.Com From: ota@sun3.oulu.fi (Olli Taikina-aho) Subject: point counter needed Hello friends Could someone tell me if there is any company delivering mechanical point counter systems. I need it for a polarizing microscope for geological samples. Olli Taikina-aho University of Oulu Institute of Electron Optics Box 400 SF-90571 Oulu ota@cc.oulu.fi tel. +358-81-5533142 fax. +358-81-5533149 From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 3-APR-1996 06:40:59.13 To: MICROARCHIVE CC: Subj: unsubscribe Message-Id: <199604031024.KAA25184@worldxs.worldaccess.nl> Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Wed, 3 Apr 1996 12:24:27 +0100 To: microscopy@Sparc5.Microscopy.Com From: houpt@worldaccess.nl (houpt) Subject: unsubscribe unsubscribe BIOMET P.M.Houpt The Hague. The Netherlands. From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 3-APR-1996 10:30:52.18 To: MICROARCHIVE CC: Subj: subscribe Date: Wed, 03 Apr 1996 09:16:24 +0800 (U) From: French Michele H Subject: subscribe To: Microscopy listserver Message-id: X-Mailer: Mail*Link SMTP-MS 3.0.3 b1 d0 Content-transfer-encoding: 7BIT Suscribe french_michele-h.PRILVMS3@MSMAIL.BMS.COM From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 3-APR-1996 12:20:18.69 To: MICROARCHIVE CC: Subj: BiOS '97 Call For Papers Date: Wed, 3 Apr 1996 10:41:40 -0600 From: "Jose'Angel Conchello" Message-Id: <9604031641.AA03559@abbe> To: Microscopy@Sparc5.Microscopy.Com Subject: BiOS '97 Call For Papers Cc: josec@ibc.wustl.edu Three-Dimensional Microscopy: Imaging Acquisition and Processing IV Conference Chairs: Carol J. Cogswell, Univ. of Sydney (Australia); Jose-Angel Conchello, Washington Univ.; Tony Wilson, Univ. of Oxford (UK) Program Committee: Alan Bearden, Univ. of California/Berkeley; G. J. Brakenhoff, Univ. of Amsterdam (Netherlands); Kjell Carlsson, Royal Institute of Technology (Sweden); ; Seth Goldstein, National Institutes of Health; Gordon S. Kino, Stanford Univ.; Andres Kriete, Univ. Giessen (FRG); Frederick Lanni, Carnegie Mellon Univ. This conference will explore the rapidly developing field of three-dimensional microscopy. Consideration will be given to the characteristics of the overall system design, as well as to the topics of image formation, image recording, deconvolution in 2,3 or more dimensions, and digital methods of producing and displaying the resulting 3D reconstruction. Recent innovations in 3D microscopy are having a large impact in the biological and medical fields, as well as in materials science and the semiconductor industry. Many microscopes are now fully integrated systems, including computer hardware and software. It is hoped that the broad range of relevant topics being presented at this symposium will serve to encourage interaction among instrumentation engineers, computer image analysts, and researchers in the various fields of application. Papers are invited in the following and related areas: - confocal microscopy - 3D image formation - instrumentation for 3D microscopy - time-resolved image acquisition systems - image processing and analysis - 3D image reconstruction - deconvolution in 2/3 or more dimensions - super-resolution - spatiotemporal reconstruction of living cells and tissues - applications of 3D microscopy in materials science - profilometry - image visualization techniques for 3D microscopy systems, including volume rendering, animation, stereoscopic and holographic displays This conference is just one of 27 topics to be held at the BiOS '97 symposium, which is part of the larger event called Photonics West '97: 8-14 February 1997 San Jose Convention Center San Jose, California USA TO OBTAIN ALL CALLS FOR PAPERS ELECTRONICALLY The calls for papers for all conferences in the BiOS '97 symposium (as well as all other symposia that are part of Photonics West) will be available 1 May on the SPIE Web site http://www.spie.org/web/meetings/calls/submissions.html; by anonymous FTP ftp://spie.org/meetings/calls/PW97_readme.txt; or by e-mail file retrieval send a message to info-spie-request@spie.org with the following in the message body: send [meetings.calls]PW97_readme.txt For a printed BiOS '97 call for papers or other information: E-mail: PW97@spie.org Fax: 360/647-1445 Phone: 360/676-3290 IMPORTANT DEADLINES Paper Abstracts Due from Authors: 15 July 1996 (post-meeting proceedings) Advance Programs due from Chairs: 12 August 1996 (post-meeting proceedings) Camera-Ready Abstracts due from Authors: 9 December 1996 Manuscripts Due from Authors: 13 January 1997 (post-meeting proceedings) GUIDELINES FOR SUBMITTING AN ABSTRACT Send a 250 word abstract of your paper, by 15 July 1996, in ONE of the following ways: * SPIE WEB - Complete the convenient form found at SPIE Web site: http://www.spie.org/web/meetings/calls/submissions.html * or E-MAIL each abstract separately to: abstracts@spie.org in ASCII text (not encoded) format. To ensure receipt and proper processing of your abstract, write on SUBJECT line: PW '97 and Conference Chair. Example: SUBJECT: PW97 (John Smith) * or MAIL three copies of your abstract to: BiOS '97 SPIE, P.O. Box 10, Bellingham, WA 98227-0010 USA Shipping address: 1000 20th St., Bellingham, WA 98225 USA Telephone 360/676-3290 * or FAX one copy to SPIE at 360/647-1445 (send each abstract separately). Be sure each abstract includes the following: 1. CONFERENCE CHAIR and CONFERENCE TITLE (submit to ONLY ONE conference) to which the abstract is submitted 2. AUTHOR LISTING (List principal author first) for each author: full name and affiliation, mailing address, phone/fax numbers, email 3. ABSTRACT/PAPER TITLE 4. ABSTRACT TEXT: 250 words 5. KEYWORDS: maximum of 5 keywords 6. BRIEF BIOGRAPHY of the principal author: 50-100 words Please contact SPIE if you have any questions or require further information. Marilyn E. Gorsuch, Technical Programs Manager SPIE PO Box 10 1000-20th Street Bellingham, WA 98225 Ph: 360/676-3290 Fax: 360/647-1445 e-mail: marilyn@spie.org From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 3-APR-1996 13:34:45.36 To: MICROARCHIVE CC: Subj: Sprinklers in the EM Lab Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Wed, 3 Apr 1996 12:47:28 -0500 To: Microscopy@Sparc5.Microscopy.Com From: David.Rothbard@ipst.edu (David Rothbard) Subject: Sprinklers in the EM Lab Like many of you , I have worked in EM laboratories with water (fire activiated) sprinklers overhead. In some cases I have successfully lobbied to have them removed, as I felt there was a greater danger of electrical shock or massive instrument damage if they tripped in error (beware the pipefitter). However, there is always the possibility that your EM could be the source of a fire that causes great damage and injury. How do people feel about this? David Rothbard -- Institute of Paper Science and Technology From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 3-APR-1996 14:39:19.50 To: MICROARCHIVE CC: Subj: unsubscribe From: samso@orkney.ph.albany.edu Date: Wed, 03 Apr 1996 13:28:24 EST To: microscopy@aaem.amc.anl.gov Message-ID: <009A04E9.E61EBDAA.8@tethys.ph.albany.edu> Subject: unsubscribe unsubscribe From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 3-APR-1996 15:44:42.72 To: MICROARCHIVE CC: Subj: Re: point counter needed Message-ID: <3162D750.4D11@cpcug.org> Date: Wed, 03 Apr 1996 11:53:52 -0800 From: "John M. Libert" Organization: Capital PC User Group X-Mailer: Mozilla 2.0 (Win16; I) MIME-Version: 1.0 To: Olli Taikina-aho CC: microscopy@Sparc5.Microscopy.Com Subject: Re: point counter needed References: <199604030642.JAA23290@cc.oulu.fi> Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Olli Taikina-aho wrote: > > Hello friends > > Could someone tell me if there is any company delivering mechanical point > counter systems. I need it for a polarizing microscope for geological samples. > Olli Taikina-aho > University of Oulu > Institute of Electron Optics > Box 400 > SF-90571 Oulu > ota@cc.oulu.fi > tel. +358-81-5533142 > fax. +358-81-5533149Yes. Prior Scientific, Inc of Rockland, MA sells an electro-mechanical pointcounter. It has a number of nice features and is fairly simple. Give them a call at 617-878-8442 (FAX 617-878-8736). John M. Libert OPELCO OPtical ELements COrporation Sterling, VA. 703-471-0080 Ext. 217 From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 3-APR-1996 16:32:28.00 To: MICROARCHIVE CC: Subj: HELP Unsubscribe Date: Wed, 3 Apr 1996 15:24:03 -0500 (EST) From: Leslie Gartner To: Philip Oshel Cc: microscopy@Sparc5.Microscopy.Com Subject: HELP Unsubscribe In-Reply-To: Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII I apologize for posting this to the entire community, but I no longer have the majordomo address to unsubscribe nor the proper procedure. Please e-mail me directly to reduce bandwidth clutter. Thank you, Les Gartner lgartner@umabnet.ab.umd.edu From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 3-APR-1996 17:22:05.92 To: MICROARCHIVE CC: Subj: Re: Sprinklers in the EM Lab Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Wed, 3 Apr 1996 16:00:50 -0500 To: Microscopy@Sparc5.Microscopy.Com From: David.Rothbard@ipst.edu (David Rothbard) Subject: Re: Sprinklers in the EM Lab Several people have mentioned Halon to me, but I was under the impression that Halon has fallen out of favor due to potential toxicity and suffocation. David Rothbard -- Institute of Paper Science and Technology From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 3-APR-1996 18:22:42.48 To: MICROARCHIVE CC: Subj: Re: Sprinklers in the EM Lab Date: Wed, 3 Apr 1996 15:51:49 -0600 (CST) From: rnessler Subject: Re: Sprinklers in the EM Lab To: David Rothbard cc: Microscopy@Sparc5.Microscopy.Com In-Reply-To: Message-ID: MIME-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII On Wed, 3 Apr 1996, David Rothbard wrote: > Like many of you , I have worked in EM laboratories with water (fire > activiated) sprinklers overhead. In some cases I have successfully lobbied > to have them removed, as I felt there was a greater danger of electrical > shock or massive instrument damage if they tripped in error (beware the > pipefitter). However, there is always the possibility that your EM could > be the source of a fire that causes great damage and injury. How do people > feel about this? > > David Rothbard > > -- > Institute of Paper Science and Technology > Our lab is equipped with a Halon fire suppression system. I can't verify that it works, as we have never needed it :) Randy Nessler rnessler@emiris.iaf.uiowa.edu From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 3-APR-1996 19:18:47.38 To: MICROARCHIVE CC: Subj: LR White Date: Wed, 3 Apr 1996 12:04:28 -0600 (CST) From: Joyce Craig Subject: LR White To: microscopy listerver Message-ID: MIME-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII We are using LR white for the first time in anticipation of doing some immunocytochemistry and also because I have several people who are working with bacteria and fungi. We have been fixing the bacteria in 2 + 2, trying different concentrations of buffer because the bacteria grows in a 25% sodium chloride. Then we mix the bacteria with agar, fix again, osmicate, and dehydrate to 70%. Then we mix the LR White with 70 % for infiltration, then straight LR White finally flat embed in LR White and polymerize at 60 degreesC. We wrap the embedding molds in Saran wrap (not an off-brand) before polymerization. The blocks in blue JB4 molds hardened, but have a surface stickiness. The blocks in the clear molds disappeared. The black blocks of osmicated agar are there but the epoxy is gone. Does anyone have any thoughts on this? I really like running up nice blocks of pancreas and kidney and even cardiac muscle in epoxy and taking pretty pictures in the microscope but unfortunately no-one here is doing this nice rewarding basic work. From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 3-APR-1996 20:13:37.76 To: MICROARCHIVE CC: Subj: Unsubscribe Date: Wed, 3 Apr 1996 17:31:37 -0800 Message-Id: <199604040131.RAA01712@felix.scvnet.com> X-Sender: vit@felix.scvnet.com X-Mailer: Windows Eudora Version 1.4.3 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" To: microscopy@Sparc5.Microscopy.Com From: vit@felix.scvnet.com (Jim Yankovich) Subject: Unsubscribe Please Unsubscribe From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 3-APR-1996 21:16:21.07 To: MICROARCHIVE CC: Subj: Postdoc - TEM, fluorescence (maybe confocal) Date: Thu, 04 Apr 1996 09:16:15 +1200 From: rgwhite@vaxc.cc.monash.edu.au (Rosemary White) Subject: Postdoc - TEM, fluorescence (maybe confocal) To: CONFOCAL@UBVM.CC.BUFFALO.EDU Cc: cytonet@net.bio.net, MICROSCOPY@Sparc5.Microscopy.Com Message-id: MIME-version: 1.0 Content-type: text/plain; charset="us-ascii" Content-transfer-encoding: 7BIT POSTDOCTORAL FELLOWSHIP IN PLANT CELL BIOLOGY Applications are invited for a postdoctoral fellowship on a 3-year project investigating computer modelling of macromolecular structures in plant cells. Part of this work requires preparation of TEM and immunofluorescence micrographs for comparison with computer generated images of plant cytoskeleton and plasmodesmata. We are also investigating various aspects of the interactions between these cell components and their dynamic behaviour in cells. Some confocal fluorescence microscopy may be required. Applicants should have experience in TEM and immunofluorescence of plant material. Experience in freeze-substitution techniques and immunofluorescence of the plant cytoskeleton would be an advantage. The position is available from 1 July, and the successful candidate will be expected to take up the appointment by 1 September 1996 at the latest. Salary will commence at $(Australian)37,345. The appointment will be for 12 months in the first instance, renewable for a further 12 months (longer if new grant application is successful). Some remuneration for removal expenses will be available. Applications should arrive by 4 May 1996, with CV naming 2 referees. For further information, contact Rosemary White __ / Department of Ecology _/ \__/ \ and Evolutionary Biology / \ Monash University / Australia \ Clayton, Victoria 3168 \ ____ / phone 61-3-9905 5670 \_/ \_*_/ fax 61-3-9905 5613 __ email rgwhite@vaxc.cc.monash.edu.au \/ From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 3-APR-1996 22:13:34.09 To: MICROARCHIVE CC: Subj: immuno:source of antibodies Date: Wed, 3 Apr 1996 21:21:55 -0500 (EST) From: Sarka Lhotak To: Microscopy Subject: immuno:source of antibodies Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Dear immunobuffs, I was very impressed with the ChemExpo www site that was recommended here recently. I wonder whether there is something similar from antibody producers that would enable to locate a source of a particular antibody easily. My search for this was unsuccessful. Thanks for your help. Sarka Lhotak EM Facility, McMaster University Hamilton, Ontario, Canada lhotaks@fhs.mcmaster.ca From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 3-APR-1996 23:18:12.15 To: MICROARCHIVE CC: Subj: Re: Sprinklers in the EM Lab Date: Wed, 03 Apr 96 17:16:24 PST From: "Bob Citron" Message-Id: <9603038285.AA828580773@cc.chiron.com> To: Microscopy@Sparc5.Microscopy.Com, David.Rothbard@ipst.edu (David Rothbard) Subject: Re: Sprinklers in the EM Lab David; A few years ago, we had a very bad rainstorm in Southern California. The roof of our R&D building (which houses the SEM) leaked, and the ceiling tile got so soaked that it fell in on the SEM console. When I came in the next morning, there was a steady stream of water pouring into the keyboard and down into the electronics of the SEM. Obviously, the SEM had tripped off. I unplugged it and called the vendor (Cambridge, at the time), since we were under a service contract. End result - we allowed it to dry for 3 days and fired it up. It worked fine, and has been operational ever since; no problems have been attributed to this incident. In summary, I believe that the risks associated with fire far outweigh the risks associated with water. Regards, Bob ***************************** Bob Citron Chiron Vision 555 W. Arrow Hwy Claremont, CA 91711 (909)399-1311 Bob_Citron@cc.chiron.com ***************************** ______________________________ Reply Separator _________________________________ Subject: Sprinklers in the EM Lab Author: David.Rothbard@ipst.edu (David Rothbard) at SMTP Date: 4/3/96 3:48 PM Like many of you , I have worked in EM laboratories with water (fire activiated) sprinklers overhead. In some cases I have successfully lobbied to have them removed, as I felt there was a greater danger of electrical shock or massive instrument damage if they tripped in error (beware the pipefitter). However, there is always the possibility that your EM could be the source of a fire that causes great damage and injury. How do people feel about this? David Rothbard -- Institute of Paper Science and Technology From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-APR-1996 08:19:09.82 To: MICROARCHIVE CC: Subj: Re: Sprinklers in the EM Lab From: "Crossman, Harold" To: "'Microscopy'" Subject: Re: Sprinklers in the EM Lab Date: Thu, 04 Apr 96 07:42:00 PST Message-Id: <3163F0CB@hq_smtp> Encoding: 36 TEXT X-Mailer: Microsoft Mail V3.0 I agree. I have had equipment go through fires and floods. Given the choice, I would rather have wet equipment that will dry out than fried equipment or worse, fried people. Harold Crossman ---------- From: Microscopy-request To: Microscopy; David.Rothbard Subject: Re: Sprinklers in the EM Lab Date: Wednesday, April 03, 1996 5:16PM David; A few years ago, we had a very bad rainstorm in Southern California. The roof of our R&D building (which houses the SEM) leaked, and the ceiling tile got so soaked that it fell in on the SEM console. When I came in the next morning, there was a steady stream of water pouring into the keyboard and down into the electronics of the SEM. Obviously, the SEM had tripped off. I unplugged it and called the vendor (Cambridge, at the time), since we were under a service contract. End result - we allowed it to dry for 3 days and fired it up. It worked fine, and has been operational ever since; no problems have been attributed to this incident. In summary, I believe that the risks associated with fire far outweigh the risks associated with water. Regards, Bob From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-APR-1996 09:37:44.48 To: MICROARCHIVE CC: Subj: Re: immuno:source of antibodies Message-ID: <85C4633101F70300@mhs.unc.edu> In-Reply-To: <7DC4633101F70300> Date: Thu, 4 Apr 1996 8:07:25 -0500 From: "Schoonhoven, Robert" Sender: "Schoonhoven, Robert" Organization: UNC To: lhotaks@fhs.csu.mcmaster.ca (Sarka Lhotak) Cc: microscopy@Sparc5.Microscopy.Com Subject: Re: immuno:source of antibodies X-Mailer: Connect2-SMTP 4.00.b27E MHS to SMTP Gateway I haven't found anything on the net but an excellent (and not to expensive ) refferance is "Linscotts Directory of Immunological and Biological Reagents" the send out constant updates and is available in written and PC compatible form. The phone # for additional information is 707-544-9555. regards, bob Robert Schoonhoven Laboratory of Molecular Carcinogenesis and Mutagenesis University of North Carolina CB#7400, Rosenau Hall Chapel Hill, NC 27599-7400 Ph. 919-966-6343 Fax 919-966-6123 From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-APR-1996 10:18:13.39 To: MICROARCHIVE CC: Subj: Water and the SEM From: "Craig, Bob" To: Microscopy Forum Subject: Water and the SEM Date: Thu, 04 Apr 96 07:56:00 PST Message-Id: <3163F416@hq_smtp> Encoding: 19 TEXT X-Mailer: Microsoft Mail V3.0 Good Morning All, Bob Citron's response to David Rothbard reminded me on an incident we had with an AMRAY 1000 many years ago (20?). A cooling water line on the diffusion pump failed and filled the Plexiglass housing of the high voltage supply with treated cooling water. Upon discovery me heart entered my throat and visions of disaster entered my mind. As in Bob's case, the service engineer calmly drained the water, rinsed the power supply with deionized water and dried it with a hair dryer. Other component also have been wet, which he dried with Kim Wipes. When he fired it up I expected sparks to fly, etc. No way!! The microscope worked like nothing had happened. Today the scope is 23 or 24 years old and still running! These experiences would indicate that older analog instruments were pretty hardy. How a modern digital instrument would react to such treatment is a good question. Bob Craig OSRAM SYLVANIA INC. Beverly, MA 01915 craig@rd.sylvania.com From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-APR-1996 11:22:12.50 To: MICROARCHIVE CC: Subj: Re: LR White Date: Thu, 4 Apr 1996 08:20:06 GMT Message-Id: <199604040820.IAA14617@snitch.biotech.ufl.edu> X-Sender: sdw@biotech.ufl.edu X-Mailer: Windows Eudora Light Version 1.5.2 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" To: Joyce Craig From: Scott Whittaker Subject: Re: LR White Cc: microscopy@Sparc5.Microscopy.Com I maintain an archive of most of the biological EM related material that comes across this list. Since you have net acess I will assume you can get acess to the www. Go to the web page listed at the bottom of this message and click of the "Tips & Tricks Wizard" In there is a file called "Embedding with LR White" which may be of use to you. If for some reason you do not have acess to the web or cannot read the file, let me know and I will e-mail it to you . At 12:04 PM 4/3/96 -0600, you wrote: >We are using LR white for the first time in anticipation of doing some >immunocytochemistry and also because I have several people who are >working with bacteria and fungi. >We have been fixing the bacteria in 2 + 2, trying different >concentrations of buffer because the bacteria grows in a 25% sodium >chloride. Then we mix the bacteria with agar, fix again, osmicate, and >dehydrate to 70%. Then we mix the LR White with 70 % for infiltration, then >straight LR White finally flat embed in LR White and polymerize at 60 >degreesC. >We wrap the embedding molds in Saran wrap (not an off-brand) before >polymerization. >The blocks in blue JB4 molds hardened, but have a surface stickiness. >The blocks in the clear molds disappeared. The black blocks of osmicated >agar are there but the epoxy is gone. >Does anyone have any thoughts on this? >I really like running up nice blocks of pancreas and kidney and even >cardiac muscle in epoxy and taking pretty pictures in the microscope but >unfortunately no-one here is doing this nice rewarding basic work. > > ><><><><><><><><><><><><><><><><><><><><><><><><><>< Scott D. Whittaker 218 Carr Hall Research Assistant Gainesville, FL 32610 University Of Florida ph 904-392-1295 ICBR EM Core Lab fax 904-846-0251 sdw@biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/ From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-APR-1996 12:27:32.39 To: MICROARCHIVE CC: Subj: answers to: need books on biology... Date: Thu, 4 Apr 1996 15:12:50 GMT Message-Id: <9604041512.AA33100@risc990.bologna.enea.it> X-Sender: giocar@risc990.bologna.enea.it (Unverified) X-Mailer: Windows Eudora Pro Version 2.1.2 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" To: Microscopy@Sparc5.Microscopy.Com From: Giorgio Subject: answers to: need books on biology... Following my request for more details, Ms Kaye Patterson has sent me more information about a book I think can be very interesting for many people. For its possible general interest, I send this message to the list. Greetings George ---------------------------------------------------------- George, ... A company in America called Ward's Biology has a very large catalogue which they send to us here in Australia every year. Their address is P.O. Box 92912 Rochester, NY 14692-9012. For International Customers: Fax: 716-334-6174 Phone 716-359-2502. They have a large selection of Biological books, manuals, guides, etc. which you would probably find useful, as well as models, equipment, charts, microscope slides etc. etc. etc. They have the book you are asking about available for US$65.00, Catalogue No. 32 W 0902. The full details of the book are: Morholt, Evelyn and Brandwein, Paul F. (1986) A Sourcebook for the Biological Sciences. 3rd ed. The publisher of my 2nd ed. copy is Harcourt, Brace & World, Inc. New York, but it was published in 1966 (and also has Alexander Joseph as a third author), so I don't know what the publishing details for the 3rd ed. are. The Ward's catalogue says of the book "No biology teacher should be without this comprehensive sourcebook of techniques, procedures, demonstrations, projects, experiments and support material. Also includes sections on maintaining organisms, safety and chemical preparations. Hardcover, 813 pages, with illustrations." I can only comment about the book as a biology technician and not as a teacher. I find it useful as the book details activities and experiments which our Uni students do, along with the practical aspects which I need to get the classes ready. eg. for classes looking at Protozoans, invertebrates, etc., the book describes the best way to get material from the field, how to culture the various groups, how to prepare material for microscopic examination, anesthetization, staining and fixation techniques. The book covers many areas of Biology - anatomy, cells & tissues, photosynthesis, ingestion & digestion, transpiration, circulation, respiration, behaviour & coordination, genetics, growth & differentiation, evolution, and ecology. There is a section on special techniques - Maintaining animals useful in the classroom, Growing plants useful in the classroom and Stockroom and facilities for biology. This is the section I use the most. Hope you find all this useful. Kaye From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-APR-1996 14:05:32.39 To: MICROARCHIVE CC: Subj: Sprinklers in the EM Lab From: dbd1@uclink4.berkeley.edu Date: Thu, 4 Apr 1996 09:30:43 -0800 (PST) Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" X-mailer: Eudora Pro 2.1.3 To: microscopy@aaem.amc.anl.gov Subject: Sprinklers in the EM Lab Halon is great for stopping fires but yes, the same mechanism that extinguishes the fire will suffocate people. Sprinklers are generally OK but I prefer a standard A-B-C rated fire extinguisher in the scope room. We have had "umbrellas" mounted over the scopes for the last 20 years- aluminum tube framework covered by a heavy-duty waterproof nylon fabric. We reside in an old building and very occasionally, plumbing accidents result in water leaking throught the ceiling above the scope. Regards, Doug Davis Staff Research Associate Electron Microscope Facility University of California Berkeley, CA 94720 (510) 642-2085, fax 643-6207 dbd1@uclink4.berkeley.edu From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-APR-1996 14:38:49.65 To: MICROARCHIVE CC: Subj: EM and Water X-Sender: bozzola@saluki-mail.fiber2.siu.edu Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Thu, 4 Apr 1996 12:04:35 -0600 To: microscopy@aaem.amc.anl.gov From: bozzola@siu.edu (John. J. Bozzola) Subject: EM and Water I would venture that analog instruments might be able to weather the onslaught of water better than newer instruments with lots of sensitive electronics. The best approach would be to contact computing centers and see what measures are taken to protect their instrumentation. Secondly, some sort of risk assessment needs to be considered here. What are the odds that floods will be generated needlessly versus having a facility or instrumentation destroyed by fire? Most instances of fires affecting EM units that I have heard about were caused by fires originating other than in the EM room. Perhaps sprinklers could be used in locations other than the EM while the EM rooms could be protected by more electronically-friendly agents (Halon, carbon dioxide, etc). Just my thoughts ..... ############################################################################# John J. Bozzola, Ph.D., Director Center for Electron Microscopy Southern Illinois University Carbondale, IL 62901-4402 U.S.A. Phone: 618-453-3730 Fax: 618-453-2665 Email: bozzola@siu.edu Web: http://www.siu.edu/departments/shops/cem.html ############################################################################# From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-APR-1996 14:38:51.35 To: MICROARCHIVE CC: Subj: EM and Water X-Sender: bozzola@saluki-mail.fiber2.siu.edu Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Thu, 4 Apr 1996 12:04:35 -0600 To: microscopy@aaem.amc.anl.gov From: bozzola@siu.edu (John. J. Bozzola) Subject: EM and Water I would venture that analog instruments might be able to weather the onslaught of water better than newer instruments with lots of sensitive electronics. The best approach would be to contact computing centers and see what measures are taken to protect their instrumentation. Secondly, some sort of risk assessment needs to be considered here. What are the odds that floods will be generated needlessly versus having a facility or instrumentation destroyed by fire? Most instances of fires affecting EM units that I have heard about were caused by fires originating other than in the EM room. Perhaps sprinklers could be used in locations other than the EM while the EM rooms could be protected by more electronically-friendly agents (Halon, carbon dioxide, etc). Just my thoughts ..... ############################################################################# John J. Bozzola, Ph.D., Director Center for Electron Microscopy Southern Illinois University Carbondale, IL 62901-4402 U.S.A. Phone: 618-453-3730 Fax: 618-453-2665 Email: bozzola@siu.edu Web: http://www.siu.edu/departments/shops/cem.html ############################################################################# From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-APR-1996 15:37:24.52 To: MICROARCHIVE CC: Subj: Re: Temperature Controller Date: Thu, 4 Apr 1996 12:52:38 -0600 (CST) From: Weiming Yu To: microscopy@Sparc5.Microscopy.Com Subject: Re: Temperature Controller Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Hi Friends, Thank your all for your responses. That is a great help. Weiming Yu Ph.D. Dept of Physics, UIUC 1110 W. Green Street Urbana, IL 61801 w-yu@uiuc.edu From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-APR-1996 17:08:54.37 To: MICROARCHIVE CC: Subj: Re: Sprinklers in the EM Lab From: wise@vaxa.cis.uwosh.edu Date: Thu, 04 Apr 1996 09:46:53 +0000 Subject: Re: Sprinklers in the EM Lab To: Bob Citron Cc: Microscopy@sparc5.microscopy.com Message-id: <01I3512JZNSI001QZ7@VAXA.CIS.UWOSH.EDU> MIME-version: 1.0 Content-type: text/plain; charset="us-ascii" Content-transfer-encoding: 7BIT >David; > >A few years ago, we had a very bad rainstorm in Southern California. The roof >of our R&D building (which houses the SEM) leaked, and the ceiling tile got so >soaked that it fell in on the SEM console. When I came in the next morning, >there was a steady stream of water pouring into the keyboard and down into the >electronics of the SEM. Obviously, the SEM had tripped off. I unplugged it >and >called the vendor (Cambridge, at the time), since we were under a service >contract. End result - we allowed it to dry for 3 days and fired it up. It >worked fine, and has been operational ever since; no problems have been >attributed to this incident. In summary, I believe that the risks associated >with fire far outweigh the risks associated with water. Yes, but rainwater is clean. I have been told (third- or fourth-hand story by now) that it is not the water in sprinkler systems as much as the rust, junk and (perhaps) rust inhibitors in the pipes that cause the major damage. Bob Wise From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-APR-1996 17:09:52.57 To: MICROARCHIVE CC: Subj: Re: Sprinklers in the EM Lab From: William Tivol Message-Id: <199604041424.JAA20254@elba> Subject: Re: Sprinklers in the EM Lab To: David.Rothbard@ipst.edu (David Rothbard) Date: Thu, 4 Apr 1996 09:24:27 -0500 (EST) Cc: microscopy@Sparc5.Microscopy.Com In-Reply-To: from "David Rothbard" at Apr 3, 96 12:47:28 pm X-Mailer: ELM [version 2.4 PL21] Content-Type: text Content-Length: 1109 > Like many of you , I have worked in EM laboratories with water (fire > activiated) sprinklers overhead. In some cases I have successfully lobbied > to have them removed, as I felt there was a greater danger of electrical > shock or massive instrument damage if they tripped in error (beware the > pipefitter). However, there is always the possibility that your EM could > be the source of a fire that causes great damage and injury. How do people > feel about this? > Dear David, We have had one fire in an EM room--started when the LN delivery system ran dry and a solenoid valve shorted out. There are definitely other potential fire dangers, such as vacuum pumps. We do not have sprinklers. I have not heard of erronious tripping of sprinkeler sys- tems being a particular problem. All-in-all, I lean toward having a fire supression system, but there are obvious problems with a water- based one, even if it trips due to a real fire. Is there a better system for areas where large voltages and/or currents are present? Perhaps some industries have solved this problem. Yours, Bill Tivol From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-APR-1996 17:58:54.21 To: MICROARCHIVE CC: Subj: Re: EM and Water Date: Thu, 4 Apr 1996 16:34:35 -0500 (EST) From: Jay Jerome X-Sender: jjerome@isnet To: Microscopy Forum Subject: Re: EM and Water In-Reply-To: Message-ID: MIME-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII With regard to the thread about fire extinquishers in EM suites, we were told during our last safety inspection that we would need to get rid of our halon system the next time it needed a charge. This was mostly because the insurance companies would no longer cover such things. They require springler systems. They would rather pay the for lost equipment than the hazards of halon. Of course our safety officer had no answer to my question of whether the school would pay the 5,000 deductible or whether I would have to pay this. Jay Jerome ************************************************************** * aka: W. Gray Jerome * * Dept. of Pathology * * Bowman Gray School of Medicine of Wake Forest University * * Medical Center Blvd * * Winston-Salem, NC 27157-1092 * * 910-716-4972 * * jjerome@bgsm.edu * ************************************************************** From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-APR-1996 18:24:49.42 To: MICROARCHIVE CC: Subj: LN2 Safety Date: Thu, 4 Apr 1996 08:54:30 -0600 Message-Id: <199604041454.IAA20174@Sparc5.Microscopy.Com> Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" To: Microscopy@Sparc5.Microscopy.Com From: emccjb@ttuhsc.edu (Charles J. Butterick) Subject: LN2 Safety Greetings, I've read with interest the comments of others questioning liquid nitrogen safety regulations. In spite of many years of pratical experience with the stuff and being a trained safety officer, I took some time to examine the subject. I disagree with those who have problems with the regulations. The regs seem to be aimed at preventing continuous contact and the resultant injuries. In the CRC Handbook of Laboratory Safety, 3rd edition, pages 315-317, lists all the basic precautions (face shield, impervious clothing, etc.). For those who disagree, gloves are optional.....except, as Tivol pointed out, when in contact with cold metal parts. The CRC actually suggests using some sort of potholder to protect hands from the metal. The CRC also points out that efforts should be made to prevent trapping the cryogen. When trapped against the skin, LN2 will cause injury since the contact is no longer momentary and becomes continuous contact. Cuffless pants worn over the shoes/boots are reccommended, i.e.spilled LN2 that gets trapped inside a shoe with the foot may cause injury. For those who suggest sandals as appropriate should think about the possibility that with a major spill, some LN2 could be trapped beneath the foot or under the strap of a sandal, possibly causing injury. Appropriate shoes can protect the foot for short periods. If the footwear is frozen, generally it can be removed before injury occurs. If gloves are used, the fit should be loose so that they may be shed quickly...ala a hockey player's actions when he has been treated less than courteously by an opposing player. The problem I have with faculty and staff is the use of latex gloves while using LN2. The Leidenfrost effect can definitely cause injury if the latex freezes against the skin. For those who would not wear appropriate gloves, have you considered the possibility of LN2 being trapped under a watch band, or under a ring? In a major splash, could LN2 become trapped at the beltline, between the pants and shirt? The whole idea is to make sure that most any cryogen does not get trapped against the skin. If you can handle LN2 safely without protection, or have done it for years without incident, more power to you. I hope your luck holds out. Interestingly enough, Air Liquide, a national supplier of cryogens, also lists similar precautions in the use of liquid nitrogen and other cryogens. Texas Tech has (arguably) a large number of beautiful women, but they are not the ones coming to get liquid nitrogen....and of the individuals who do get LN2 from our lab, I would rather them come clothed. Charles J. Butterick (Chuck) Electron Microscopy Center Department of Cell Biology and Biochemistry Texas Tech University Health Sciences Center 3601 4th Street Lubbock, Texas 79430 vox (806) 743-1633 fax (806) 743-1219 email emccjb@ttuhsc.edu or chuck@micron1.lubb.ttuhsc.edu From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-APR-1996 18:37:05.38 To: MICROARCHIVE CC: Subj: EMs and sprinklers Date: Thu, 4 Apr 1996 17:05:49 GMT Message-Id: <199604041705.RAA19070@snitch.biotech.ufl.edu> X-Sender: gwe@biotech.ufl.edu X-Mailer: Windows Eudora Version 1.4.4 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" To: Microscopy@sparc5.microscopy.com From: gwe@biotech.ufl.edu (Greg Erdos) Subject: EMs and sprinklers The sprinkler next to your scope may not be the problem. We are on the second floor, but when the sprinklers went off on the sixth floor, water eventually worked its way down to us. Luckily this happened in the daytime and we had enough time to cover done the scopes and computers. No one knew how to turn off the water so there was 6 inches on the 6th floor and ceilings collapsed on the 5th floor exactly as they are designed to do during a fire. There was no fire. @@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@ Greg Erdos Phone: 352-392-1295 Scientific Director, ICBR Electron Microscopy Core Lab 218 Carr Hall Fax: 352-846-0251 University of Florida E-mail: gwe@biotech.ufl.edu Gainesville, FL 32611 @@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@ From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-APR-1996 19:44:05.82 To: MICROARCHIVE CC: Subj: Sprinklers Date: Thu, 04 Apr 1996 11:56:26 -0500 (EST) From: BGIAMMARA@magnum.mco.edu Subject: Sprinklers To: David.rothbard@ipset.edu, Microscopy@Sparc5.Microscopy.Com Message-id: <01I355J6DYVC8Y5MGV@gemini.mco.edu> X-VMS-To: IN%"Microscopy@Sparc5.microscopy.com", IN%"David.rothbard@ipset.edu" MIME-version: 1.0 Content-type: TEXT/PLAIN; CHARSET=US-ASCII Content-transfer-encoding: 7BIT David, We had a great central laboratory in the basement of the Graduate School with a fire sprinkler above each of three electron microscopes. The Dean worried that the sprinklers would ruin the microscopes, so they got insurance on them and required that I keep large plastic tarps to cover them in case the sprinklers went off. They never did, howwever... One Saturday, we had a huge rainstorm and the Dean's secretary called me saying their seemed to be a "little problem in the lab." I rushed over there to find that the drains at the top of the stairs had filled with leaves and likewise the drain at the bottom and huge amounts of water, like a waterfall, was cascading down the steps and filling up the lab. The building power had gone off and the emergency lighting was on in the hallways. I called for the maintenance crew, but they were priortized to go save "expensive" student computers in another building. So there I was with my broom, frantically cleaning drains and pushing water, feeling like a character in Walt Disney's Fantasia. Because of this long and intense fight, the only thing we lost was the carpeting, papers and books on the lowest shelf. Saved the floor tile. The minimal rust on the feet of the EM's wasn't even noticeable. The lab was fine. Sprinklers a non-issue. The real devastation was done by the administration, later, when a new Dean said "What are electron microscopes good for?" eyeing the space and salary line he could use for a new Executive Secretary. But that's another story. Kind regards and good wishes to all, Beverly From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-APR-1996 20:28:45.65 To: MICROARCHIVE CC: Subj: Sprinklers Date: Thu, 04 Apr 1996 12:02:25 -0500 (EST) From: BGIAMMARA@magnum.mco.edu Subject: Sprinklers To: microscopy@Sparc5.Microscopy.Com Message-id: <01I355QM14808Y5MGV@gemini.mco.edu> X-VMS-To: IN%"microscopy@sparc5.microscopy.com" MIME-version: 1.0 Content-type: TEXT/PLAIN; CHARSET=US-ASCII Content-transfer-encoding: 7BIT ----- Begin message from IN%"postmaster@gemini.mco.edu" 4-Apr-96 From: IN%"postmaster@gemini.mco.edu" "PMDF Mail Server" 4-APR-1996 11:56 To: IN%"postmaster@gemini.mco.edu", IN%"BGIAMMARA@gemini.mco.edu" CC: Subj: Undeliverable mail: SMTP delivery failure Return-path: <> Received: from gemini.mco.edu by gemini.mco.edu (PMDF V4.3-7 #8091) id <01I355J900A88Y5QD6@gemini.mco.edu>; Thu, 4 Apr 1996 11:56:37 EST Date: Thu, 04 Apr 1996 11:56:37 -0500 (EST) From: PMDF Mail Server Subject: Undeliverable mail: SMTP delivery failure To: postmaster@gemini.mco.edu, BGIAMMARA@gemini.mco.edu Message-id: <01I355JDSTCI8Y5QD6@gemini.mco.edu> MIME-version: 1.0 Content-type: MULTIPART/MIXED; BOUNDARY="Boundary (ID JlM655DZrjy0J4/C5dhX7g)" --Boundary (ID JlM655DZrjy0J4/C5dhX7g) Content-type: TEXT/PLAIN; CHARSET=US-ASCII The message could not be delivered to: Addressee: David.rothbard@ipset.edu Reason: Illegal host/domain name found. --Boundary (ID JlM655DZrjy0J4/C5dhX7g) MIME-version: 1.0 Content-type: MESSAGE/RFC822 Received: from gemini.mco.edu by gemini.mco.edu (PMDF V4.3-7 #8091) id <01I355J6DYVA8Y5MGV@gemini.mco.edu>; Thu, 4 Apr 1996 11:56:26 EST Date: Thu, 04 Apr 1996 11:56:26 -0500 (EST) From: BGIAMMARA@gemini.mco.edu Subject: Sprinklers To: David.rothbard@ipset.edu, Microscopy@Sparc5.microscopy.com Message-id: <01I355J6DYVC8Y5MGV@gemini.mco.edu> X-VMS-To: IN%"Microscopy@Sparc5.microscopy.com", IN%"David.rothbard@ipset.edu" MIME-version: 1.0 Content-type: TEXT/PLAIN; CHARSET=US-ASCII Content-transfer-encoding: 7BIT David, We had a great central laboratory in the basement of the Graduate School with a fire sprinkler above each of three electron microscopes. The Dean worried that the sprinklers would ruin the microscopes, so they got insurance on them and required that I keep large plastic tarps to cover them in case the sprinklers went off. They never did, howwever... One Saturday, we had a huge rainstorm and the Dean's secretary called me saying their seemed to be a "little problem in the lab." I rushed over there to find that the drains at the top of the stairs had filled with leaves and likewise the drain at the bottom and huge amounts of water, like a waterfall, was cascading down the steps and filling up the lab. The building power had gone off and the emergency lighting was on in the hallways. I called for the maintenance crew, but they were priortized to go save "expensive" student computers in another building. So there I was with my broom, frantically cleaning drains and pushing water, feeling like a character in Walt Disney's Fantasia. Because of this long and intense fight, the only thing we lost was the carpeting, papers and books on the lowest shelf. Saved the floor tile. The minimal rust on the feet of the EM's wasn't even noticeable. The lab was fine. Sprinklers a non-issue. The real devastation was done by the administration, later, when a new Dean said "What are electron microscopes good for?" eyeing the space and salary line he could use for a new Executive Secretary. But that's another story. Kind regards and good wishes to all, Beverly --Boundary (ID JlM655DZrjy0J4/C5dhX7g)-- ----- End forwarded message From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 4-APR-1996 20:47:41.31 To: MICROARCHIVE CC: Subj: Re: Water and the SEM Date: Thu, 4 Apr 1996 13:38:04 -0600 Message-Id: <199604041938.NAA17558@puccini.crl.umn.edu> From: "Gib Ahlstrand" To: Microscopy@sparc5.microscopy.com Subject: Re: Water and the SEM In message <3163F416@hq_smtp> "Craig, Bob" writes: > > Good Morning All, > Bob Citron's response to David Rothbard reminded me on an incident we had > with an AMRAY 1000 many years ago (20?). A cooling water line on the > diffusion pump failed and filled the Plexiglass housing of the high voltage > supply with treated cooling water. Upon discovery me heart entered my > throat and visions of disaster entered my mind. As in Bob's case, the > service engineer calmly drained the water, rinsed the power supply with > deionized water and dried it with a hair dryer. Other component also have > been wet, which he dried with Kim Wipes. When he fired it up I expected > sparks to fly, etc. No way!! The microscope worked like nothing had > happened. Today the scope is 23 or 24 years old and still running! These > experiences would indicate that older analog instruments were pretty hardy. > How a modern digital instrument would react to such treatment is a good > question. > Bob Craig > OSRAM SYLVANIA INC. > Beverly, MA 01915 > craig@rd.sylvania.com First of all, I'm very glad for Bob & Bob that water on their SEM's did not cause any serious problems that simple drying out did not cure. But I must relay my experience with a wet SEM lest folks get the impression that these instuments are waterproof (no one would ever REALLY think that would they?). In past years, we have had water accidents in labs over our heads, in some cases two floors above, and water has leaked down through cracks in their concrete floors, through our ceiling, and onto our instruments (kind of like magnetic atraction). In one case enough water got into the SEM so that a circuit board that runs the Magnification readout and a few others were damaged a bit. Even after replacing some transistors, the boards still blew out when powered up again. My service engineer had to clean dirty residue left behind by the water off the boards - many boards - with ethanol and a toothbrush to get rid of ground paths causing electrical shorts. Naturally, such events are not covered by service contracts, so we sent the bill upstairs to the offending lab. Some one up there left water aspirator running all weekend, and naturally thats a good time for paper towels to fall off the wall into the sink to plug up the drain. We then built plexiglass "deflectors" over our SEM and TEM, big sheets suspended from the ceilings, to deflect any water coming down to the sides onto the floor. Since installation in about 1985 they have saved us twice. We feel we got off easy, as the potential was there for really serious big time damage. The overhead panels also prevent some dust from falling onto the instruments. And they make a great conversation piece when tours come through! If there is any potential danger in your labs from overhead water pipes or water from rooms above, I'd recommend that folks put up some kind of water sheding panels. Could save a big head ache if the water ever comes down. Gib Ahlstrand, MMS Newsletter Editor Electron Optical Facility, University of Minnesota, Dept. Plant Pathology 495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249 612-625-9728 FAX, giba@puccini.crl.umn.edu "MICROSCOPY & MICROANALYSIS 96" in Minneapolis, Minnesota, Aug.11-15, 1996 From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 5-APR-1996 02:11:54.86 To: MICROARCHIVE CC: Subj: Re: EM: Marine invertebrate texts Message-Id: <9604050608.AA00087@S_Yamane.cc.miyazaki-u.ac.jp> Date: Fri, 05 Apr 1996 15:08:06 +0900 From: Shin-ichi Yamane To: microscopy@aaem.amc.anl.gov Subject: Re: EM: Marine invertebrate texts In-Reply-To: X-Mailer: AL-Mail 1.10 Tue, 2 Apr 1996 13:20:10 +1100 richard.easingwood@stonebow.otago.ac.nz (Richard Easingwood) wrote. >Dear Microscopists, >Does anyone out there know of a textbook or any other reference material >which covers integumental ultrastructure - cuticle, epithelium and >associated structures and also oogenesis - in marine invertebrates. We have >a marine science student who is studying subclass Ascothoracida who is >having problems getting the ultrastructure info she needs. > >Thanks in advance. Richard Do you know " Compa