From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-NOV-1995 01:51:06.76 To: MICROARCHIVE CC: Subj: MAP From: "Goodhouse, Joseph" To: Micrscopy Subject: MAP Date: Thu, 26 Oct 95 14:31:00 PDT Message-ID: <308FFE89@molecular.princeton.edu> Encoding: 7 TEXT X-Mailer: Microsoft Mail V3.0 I am looking for the company that sells MAP, Mussel Adhesion Protein. Thank you. J. Goodhouse Molec. Bio.. Princeton University From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-NOV-1995 09:03:36.69 To: MICROARCHIVE CC: Subj: subscribtion on listserver Date: Wed, 1 Nov 1995 14:17:22 +0100 Message-Id: <199511011317.OAA04428@alnus.slu.se> X-Sender: michaelb@alnus.slu.se X-Mailer: Windows Eudora Light Version 1.5.2 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" To: Microscopy@Sparc5.Microscopy.Com From: Michael Bosma Subject: subscribtion on listserver would like to subscribe to the microscopy listserver kind regards, Michael Bosma ****************************************************************************** * * * Michael Bosma Telephone: +46 418 670 62 * * The Swedish University of Fax: + 46 418 670 81 * * Agricultural Sciences E-mail: Michael Bosma@vf.slu.se * * Dept of Plant Breeding Research * * S-26831 Svaloev, Sweden * * * ****************************************************************************** From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-NOV-1995 10:04:36.72 To: MICROARCHIVE CC: Subj: Re: BioRad Gold agents? Date: 01 Nov 95 08:31:55 EST From: "ENERGY BEAM SCIENCES, INC" <75767.640@CompuServe.COM> To: Microscopy Listserver Subject: Re: BioRad Gold agents? Message-ID: <951101133155_75767.640_BHQ76-3@CompuServe.COM> For the record, over a period of time the various BioRad consumables ended up first at Energy Beam Sciences, then at SPI Supplies. Please contact both of these companies with any questions about products from the old BioRad catalog. Steven Slap, Vice-President Energy Beam Sciences, Inc. From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-NOV-1995 10:59:34.83 To: MICROARCHIVE CC: Subj: Reduced Osmium Message-Id: Date: 1 Nov 1995 09:57:41 -0400 From: "Paul Webster" Subject: Reduced Osmium To: "Microscopy BB" X-Mailer: Mail*Link SMTP-QM 3.0.2 Dear colleagues, can someone help by providing a good recipe for reducing osmium tetroxide to help increase contrast in epoxy resin - embedded biological material? Is potassium ferri-, or ferro- cyanide the best? I thought I knew the answer but a post-doc here has given me doubts so we have a small wager riding on this. From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-NOV-1995 12:03:23.72 To: MICROARCHIVE CC: Subj: Re: LM:coverslip carriers? Message-ID: Date: Wed, 1 Nov 95 08:55:18 EST From: "George F Edick" Subject: Re: LM:coverslip carriers? To: "Tamara Howard in Cold Spring Harbor Laboratory" , Microscopy@Sparc5.Microscopy.Com X-Mailer: VersaTerm Link v1.1.1 Hello, I use alumina racks from Thomas Scientific (800-345-2100) that hold 12 coverslips(up to 22x32mm). The cat # is 8542-E40. The racks can also be purchased as part of a staining apparatus. George >Does anyone have a source for racks (metal, plastic...) that will hold >glass coverslips for staining? Several of us have used metal racks in the >past that would hold at least 10 coverslips - now we can only find smaller >ones in the catalogues. Preferably for 22x22mm cs, but we'll take anything >anyone sells! >Thanks! >Tamara Howard >Cold Spring Harbor Lab >howard@cshl.org > > > George Edick RPI - Dept. Biology Troy, NY 12180 edickg@rpi.edu From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-NOV-1995 13:10:10.62 To: MICROARCHIVE CC: Subj: Re: Microwave fixation Date: 01 Nov 95 08:31:45 EST From: "ENERGY BEAM SCIENCES, INC" <75767.640@CompuServe.COM> To: "John F. Mansfield" Cc: Microscopy Listserver , Gary Login Subject: Re: Microwave fixation Message-ID: <951101133144_75767.640_BHQ76-1@CompuServe.COM> John Mansfield responded to Gary Logins' post by asking, "Although not from a company this looks much like a shameless commercial to sell this guy's books, what do you think?" Gary Login is recognized as one of the leading experts in the world on this subject. I am absolutely convinced that his references to his own articles and books were made in a sincere effort to be helpful to a fellow microscopist looking for source material on a technique. This is far from the first time a microscopist has mentioned his own publications in a posting to this list (I recall even seeing ISBN numbers). By the way, I have no commercial interest in Gary's book, which is sold by some of my esteemed competitors. Steven Slap, Vice-President Energy Beam Sciences From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-NOV-1995 14:07:05.56 To: MICROARCHIVE CC: Subj: reduced osmium X-Sender: grace@popmail Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Wed, 1 Nov 1995 11:18:06 -0800 To: microscopy@Sparc5.Microscopy.Com From: kennedy@nsi.edu (grace kennedy) Subject: reduced osmium I've used potassium ferrOcyanide-but mainly as a membrane enhancer for neuronal tissues. If I need to just reduce overall darkening by the osmium I use 7% sucrose in the osmication-seems to slow down that process a bit. I did find that the ferrocyanide-osmium will destroy delicate tissues not well fixed in GLA-I measured the pH and as I recall it was quite high-around 10-11. You also have to be careful of the buffer system you are in-buffer incompatabilities will give you a fine crystalline precipitate which cannot be removed. Phosphate is out, cacodylate is OK. I have not tried this on any HRP-filled material-I have no idea whether or not it could damage the chromogen... Grace Kennedy.. From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-NOV-1995 15:07:04.39 To: MICROARCHIVE CC: Subj: Re: Reduced Osmium Date: Wed, 1 Nov 1995 13:45:36 -0500 (EST) From: "A. Kent Christensen" X-Sender: akc@centipede.rs.itd.umich.edu To: Paul Webster cc: Microscopy BB Subject: Re: Reduced Osmium In-Reply-To: Message-ID: MIME-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII While we're on this topic, could I ask a related question? Why does the usual ferri-ferrocyanide method (Karnovsky 1971, ASCB abstracts; or Russell and Burguet, 1978, Tissue and Cell 9:751) stain membranes so well, but leaves ribosomes virtually invisible? Kent A. Kent Christensen, University of Michigan, ---------------------------------------- On 1 Nov 1995, Paul Webster wrote: > Dear colleagues, > can someone help by providing a good recipe for reducing osmium tetroxide to > help increase contrast in epoxy resin - embedded biological material? Is > potassium ferri-, or ferro- cyanide the best? I thought I knew the answer but a > post-doc here has given me doubts so we have a small wager riding on this. > > From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-NOV-1995 16:13:26.87 To: MICROARCHIVE CC: Subj: Re. Microwave fixation Message-Id: X-Mailer: Novell GroupWise 4.1 Date: Wed, 01 Nov 1995 14:55:58 -0500 From: Ann-Fook Yang To: Microscopy@Sparc5.Microscopy.Com Subject: Re. Microwave fixation Thanks are due to Drs. Steven Slap and Gary Login who have responded to my posting . It was unfortunate that someone thought that Dr. Login was try to sell his book. I do not see that he has that intention. Dr. Login not only answered my questions but also suggested a number of reference to read. His effort must be highly commended. I am sure that the references and the answers will be useful for my friend and for anyone who wants to investigate more about this technique. From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-NOV-1995 16:13:58.31 To: MICROARCHIVE CC: Subj: Re: Microwave fixation Date: Wed, 1 Nov 1995 12:57:41 +0000 (GMT) From: Ray Hicks To: "John F. Mansfield" cc: MICROSCOPY@Sparc5.Microscopy.Com Subject: Re: Microwave fixation In-Reply-To: Message-ID: MIME-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Well John, I don't think it matters much. Do you think that there is such a huge bibliography on the subject that he has purposefully refrained from mentioning other books so that he can cream off the huge profits that a greater market share would give? I don't, he seems to have answered the original question fairly. John F. Mansfield wrote: > Although not from a company this looks much like a shameless commercial to > seel this guy's books, what do you think? > > >1) Rapid (i.e.,in seconds) microwave fixation is used with success to fix > >plant > >and animal tissues. In addition, several reports show the benefits of using > >microwave fixation to preserve soft tissues encased by hard shells (e.g., > >clams, > >teeth, insects). > > > >Recommended reading: > > Login, G. R., & Dvorak, A. M. (1994). Application of microwave fixation > > techniques in pathology to neuroscience studies: A review. J Neurosci > > Methods, 55, 173-182. > > Login, G. R., & Dvorak, A. M. (1994). Methods of microwave fixation for > > microscopy. A review of research and clinical applications: 1970-1992. Prog > > Histochem Cytochem, 27/4, 1-127. > > Kok, L. P., & Boon, M. E. (1992). Microwave Cookbook for Microscopists. (3rd > > ed.). Leyden: Coulomb Press. > SNIP Ray. From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-NOV-1995 17:14:41.38 To: MICROARCHIVE CC: Subj: LM:purchase of stereomicroscope X-Sender: bozzola@saluki-mail.fiber2.siu.edu Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Thu, 2 Nov 1995 03:00:42 -0600 To: microscopy@aaem.amc.anl.gov From: bozzola@siu.edu (John. J. Bozzola) Subject: LM:purchase of stereomicroscope A colleague at our institution needs to purchase a stereomicroscope (aka "dissecting" stereo scope). Needs: magnification up to 60-80x, trinoc with Polaroid camera capable of generating positive/negatives. Price limitation is $3,000. Please contact me with information. ############################################################################# John J. Bozzola, Director Center for Electron Microscopy Southern Illinois University Carbondale, IL 62901-4402 U.S.A. Phone: 618-453-3730 Fax: 618-453-2665 Email: bozzola@siu.edu Web: http://www.siu.edu/departments/shops/cem.html ############################################################################# From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-NOV-1995 18:10:03.99 To: MICROARCHIVE CC: Subj: Re: MAP Date: Wed, 01 Nov 1995 14:46:22 -0700 (MST) From: Patty Jansma Subject: Re: MAP In-reply-to: <308FFE89@molecular.princeton.edu> To: "Goodhouse, Joseph" Cc: Micrscopy Message-id: MIME-version: 1.0 Content-type: TEXT/PLAIN; charset=US-ASCII Content-transfer-encoding: 7BIT You might try Collaborative Biomedical Products at (617) 275-0004. They carry a product called Cell-Tak which I believe is very similiar to MAP. Patty Jansma Tel:602-621-6671 plj@manduca.neurobio.arizona.edu Arizona Research Labs Division of Neurobiology University of Arizona On Thu, 26 Oct 1995, Goodhouse, Joseph wrote: > > I am looking for the company that sells MAP, Mussel Adhesion Protein. > Thank you. > > J. Goodhouse > Molec. Bio.. > Princeton University From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-NOV-1995 18:54:03.65 To: MICROARCHIVE CC: Subj: Re: Reduced Osmium Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Wed, 1 Nov 1995 13:44:23 -0600 To: "Paul Webster" From: lmiller@ux1.cso.uiuc.edu (Lou Ann Miller) Subject: Re: Reduced Osmium Cc: Microscopy@Sparc5.Microscopy.Com Greetings, Both work, but the "ic" works stronger ( too strong sometimes , it gives uneven and "funny" nuclei if over stained.). I Fix first in OsO4, and at the end of the incubation, I will add 3% KCN ( "ic " variety) for 10-15 minutes. Helps greatly, and the shorter- later times keep the funny nucleus effect from happening. Lou Ann >Dear colleagues, >can someone help by providing a good recipe for reducing osmium tetroxide to >help increase contrast in epoxy resin - embedded biological material? Is >potassium ferri-, or ferro- cyanide the best? I thought I knew the answer >but a >post-doc here has given me doubts so we have a small wager riding on this. *********************** Lou Ann Miller Microscopic Imaging Laboratory College of Veterinary Medicine University of Illinois 2001 S Lincoln Ave Urbana, Illinois 61801 217-244-1566 lmiller@ux1.cso.uiuc.edu Microscopy Lab: http://www.cvm.uiuc.edu/announcements/MicSoc/MicImagLab.html Personal Home Page: http://www.cvm.uiuc.edu/HomePages/LouAnnMiller/Homepage.html *********************** From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-NOV-1995 19:48:30.65 To: MICROARCHIVE CC: Subj: Re: Microwave fixation, actually continued comment X-Sender: jfmjfm@srvr5.engin.umich.edu Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Wed, 1 Nov 1995 23:34:46 -0400 To: MICROSCOPY@Sparc5.Microscopy.Com From: jfmjfm@umich.edu (John F. Mansfield) Subject: Re: Microwave fixation, actually continued comment OK, so Gary Login's post was not commercially based. I'm sorry. I was going to send the question to Nestor Zaluzec only but I got distracted and sent it to the list by mistake. However, I think it does bear some further comment and/or discussion. This list is getting very large and there are now many cases of messages that should not be sent to all subscribers without there being some indication that a large number of them want to see the responses. It really shouldnt have been posted to the list at all, and my reasoning for that is below. Net etiquette (or Netiquette as it is typically known ) says that if there is a question in a mailing list or newsgroup then the correct response is to send a message to the original poster. Often a poster will say "private replies please, if there is enough interest I will summarize to the net/list". This should be implicit in ALL posts. In this manner if the poster receives many replies, and there is much duplication in the replies, then any summary can be edited by the original poster before sending the information out to everyone on the list/net. People wishing copies of the replies also send requests to the original poster. If the original poster geets say five to ten "me too" requests for the information You may ask why should the original poster have to do this editing and summarizing work? Well they are getting the free info courtesy of others so it isnt exactly a large price to pay. Maybe these instructions should be part of the files that gets sent to new subscribers. >Although not from a company this looks much like a shameless commercial to >sell this guy's books, what do you think? > >>1) Rapid (i.e.,in seconds) microwave fixation is used with success to fix >>plant >>and animal tissues. In addition, several reports show the benefits of using >>microwave fixation to preserve soft tissues encased by hard shells (e.g., >>clams, >>teeth, insects). >> >>Recommended reading: >> Login, G. R., & Dvorak, A. M. (1994). Application of microwave fixation >> techniques in pathology to neuroscience studies: A review. J Neurosci >> Methods, 55, 173-182. >> Login, G. R., & Dvorak, A. M. (1994). Methods of microwave fixation for John Mansfield North Campus Electron Microbeam Analysis Laboratory 413 SRB, University of Michigan 2455 Hayward, Ann Arbor MI 48109-2143 Phone: (313)936-3352 FAX (313)936-3352 jfmjfm@engin.umich.edu, John.F.Mansfield@umich.edu or jfmjfm@umich.edu they all reach me! URL: http://wwwpersonal.engin.umich.edu/~jfmjfm/jfmjfm.html From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-NOV-1995 19:56:32.14 To: MICROARCHIVE CC: Subj: Re: Microwave fixation Date: Wed, 1 Nov 1995 13:19:12 -0700 Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" To: Microscopy@Sparc5.Microscopy.Com From: chandler@lamar.ColoState.EDU (John Chandler) Subject: Re: Microwave fixation >John Mansfield responded to Gary Logins' post by asking, "Although not from a >company this looks much like a shameless commercial to >sell this guy's books, what do you think?" >Gary Login is recognized as one of the leading experts in the world on this >subject. I am absolutely convinced that his references to his own articles and >books were made in a sincere effort to be helpful to a fellow microscopist >looking for source material on a technique. >This is far from the first time a microscopist has mentioned his own >publications in a posting to this list (I recall even seeing ISBN numbers). >By the way, I have no commercial interest in Gary's book, which is sold by some >of my esteemed competitors. >Steven Slap, Vice-President >Energy Beam Sciences I'm really happy about having experts in MANY fields on this list. I'll admit that I don't know much about microwave-enhanced processing, much less the folks doing it, even though I'm on the biological side of EM. I have heard recently that there have been some very significant advances in the techniques and technology, and look forward to a lot of discussions about them. I hope everyone who has useful contributions will chime in. John chandler@lamar.ColoState.EDU From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-NOV-1995 20:56:13.00 To: MICROARCHIVE CC: Subj: Uranyl acetate and lead citrate disposal From: BARBARA.HARTMAN@1773.220.SCHERING-PLOUGH.sprint.com X400-Received: by /PRMD=SCHERING-PLOUGH/ADMD=TELEMAIL/C=US/; Relayed; Wed, 1 Nov 1995 12:16:33 -0500 X400-Received: by /PRMD=SCHERING-PLOUGH/ADMD=TELEMAIL/C=US/; Relayed; Wed, 1 Nov 1995 12:16:33 -0500 X400-Received: by /PRMD=SCHERING-PLOUGH/ADMD=TELEMAIL/C=US/; Relayed; Wed, 1 Nov 1995 12:16:33 -0500 Date: Wed, 1 Nov 1995 12:16:33 -0500 X400-Originator: BARBARA.HARTMAN@1773.220.SCHERING-PLOUGH.sprint.com X400-Recipients: MICROSCOPY@sparc5.microscopy.com X400-MTS-Identifier: [/PRMD=SCHERING-PLOUGH/ADMD=TELEMAIL/C=US/;MCC01 951101111621639781] X400-Content-Type: P2-1984 (2) Content-Identifier: Uranyl acetate a Message-ID: <"MCC01 951101111621639781*/G=BARBARA/S=HARTMAN/OU=1773/O=220/PRMD=SCHERING-PLOUGH/ADMD=TELEMAIL/C=US/"@MHS> To: PUBLIC -00000001 * Subject: Uranyl acetate and lead citrate disposal Greetings: I stain most of my EM grids by hand but for large numbers of grids, I prefer to use the Reichert Ultrostainer. Since the Ultrostainer combines the waste into one container, I am faced with finding a disposal site that will take the combined waste. It has been difficult for us in the past to dispose of our waste, but I have just been informed that now no one will accept it. The radiation sites won't take it because of the lead and the hazardous waste sites won't take it because of the uranyl. We put absolutely nothing but water down the drain so public sewers are not an option. I will be contacting Leica to see if there is a way of separating the waste, but in lieu of that, how do the rest of you dispose of this combined waste ?? Any help will be greatly appreciated. Barbara Hartman Schering-Plough Research Lafayette, NJ 07848 E-Mail: Barbara.Hartman@Schering-Plough.sprint.com From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 1-NOV-1995 21:55:06.04 To: MICROARCHIVE CC: Subj: Re: reduced osmium Message-Id: Date: 1 Nov 1995 15:11:42 -0400 From: "Paul Webster" Subject: Re: reduced osmium To: "Microscopy BB" X-Mailer: Mail*Link SMTP-QM 3.0.2 Thank you all for your replies. However, from the diverse replies, you see my problem. Here is the original reply I gave when asked: To reduce osmium from osmium (viii) to osmium (iv), the other component has be oxidised! Since iron appears in two forms Fe (iii), ferri and Fe (ii) the Fe (ii) has to be used. And Fe (ii) is ferro-. Therefore it would be reasonable to assume that ferrocyanide would be the one to use and, in fact, this is what I have been using for years with excellent results. I am mystified why others say that the ferricyanide works just as well, and I know there are are many published papers using this from respected laboratories. This just makes it all the more mysterious, or am I missing something very simple here? I thank everyone who replied, but special thanks must go to the contributors who supported me (without knowing it): George Ruben who said - "Potassium ferro- cyanide can reduce osmium tetroxide and in so doing becomes ferricyanide!" Ubirajara P. Rodrigues-Filho - "The reduction of osmium tetraoxide is probably by ferrocyanide. Ferrocyanide is oxidized to ferri reducing the osmium compound." And Walt Bobrowski, who provided the Karnovsky reference " Use of ferrocyanide-reduced osmium tetroxide in electron microscopy". ASCB meeting New Orleans, LA 1971:146. Unfortunately the stakes of the wager were not so great that they could be shared. Best regards, Paul Webster Center for Cell Imaging Yale University school of Medicine http://info.med.yale.edu/cellimg From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-NOV-1995 08:59:33.15 To: MICROARCHIVE CC: Subj: Re: Microwave fixation, actually continued comment Message-Id: <9511021258.AA08939@utkux1.utk.edu> Date: Thu, 2 Nov 1995 07:59:42 -0500 To: MICROSCOPY@Sparc5.Microscopy.Com From: rjpalmer@utkux.utcc.utk.edu (Robert J. Palmer Jr.) Subject: Re: Microwave fixation, actually continued comment Netiquette (I hate all those nerdy little "I'm a Net-insider and you're not" things like :), :(, BTW, and IMHO) also has clear guidelines on "flaming" (for non-nerdy Net users, this refers to complaints, particularly specific attacks on the originator of a posting). Net etiquette also says that the SysOp is king and it is therefore not your duty nor anyone else's to bash people who have not used to mailing list correctly. Lastly, experienced Net users don't make mistakes like sending replies to the list that are meant for the original user. Take your lumps (and they are roudly deserved as witnessed by the thread YOU generated) and let the SysOp decide when someone has stepped over the lines. Enough already! >OK, so Gary Login's post was not commercially based. I'm sorry. I was >going to send the question to Nestor Zaluzec only but I got distracted and >sent it to the list by mistake. However, I think it does bear some further >comment and/or discussion. >This list is getting very large and there are now many cases of messages >that should not be sent to all subscribers without there being some >indication that a large number of them want to see the responses. It >really shouldnt have been posted to the list at all, and my reasoning for >that is below. > >Net etiquette (or Netiquette as it is typically known ) says that if there >is a question in a mailing list or newsgroup then the correct response is >to send a message to the original poster. Often a poster will say "private >replies please, if there is enough interest I will summarize to the >net/list". This should be implicit in ALL posts. In this manner if the >poster receives many replies, and there is much duplication in the replies, >then any summary can be edited by the original poster before sending the >information out to everyone on the list/net. People wishing copies of the >replies also send requests to the original poster. >If the original poster geets say five to ten "me too" requests for the >information >You may ask why should the original poster have to do this editing and >summarizing work? Well they are getting the free info courtesy of others >so it isnt exactly a large price to pay. > >Maybe these instructions should be part of the files that gets sent to new >subscribers. > >>Although not from a company this looks much like a shameless commercial to >>sell this guy's books, what do you think? >> >>>1) Rapid (i.e.,in seconds) microwave fixation is used with success to fix >>>plant >>>and animal tissues. In addition, several reports show the benefits of using >>>microwave fixation to preserve soft tissues encased by hard shells (e.g., >>>clams, >>>teeth, insects). >>> >>>Recommended reading: >>> Login, G. R., & Dvorak, A. M. (1994). Application of microwave fixation >>> techniques in pathology to neuroscience studies: A review. J Neurosci >>> Methods, 55, 173-182. >>> Login, G. R., & Dvorak, A. M. (1994). Methods of microwave fixation for > >John Mansfield >North Campus Electron Microbeam Analysis Laboratory >413 SRB, University of Michigan >2455 Hayward, Ann Arbor MI 48109-2143 >Phone: (313)936-3352 FAX (313)936-3352 >jfmjfm@engin.umich.edu, John.F.Mansfield@umich.edu >or jfmjfm@umich.edu they all reach me! >URL: http://wwwpersonal.engin.umich.edu/~jfmjfm/jfmjfm.html From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-NOV-1995 09:48:32.16 To: MICROARCHIVE CC: Subj: PAL Laserdisk to anything From: "Richard E. Edelmann" Organization: Miami University To: microscopy@Sparc5.Microscopy.Com Date: Thu, 2 Nov 1995 08:46:20 -500 Subject: PAL Laserdisk to anything Priority: normal X-Mailer: Pegasus Mail/Windows (v1.22) Message-Id: <144BE34E55@casmail.muohio.edu> Technically not a microscopy question but an imaging question. Does anyone out there know of any source for converting a european PAL format laserdisk (of microscopic images and audio) into any common North American format (i.e. NTSC Video tape or CD-ROM) so we can use the disk as a teaching tool? (The company that sells the disk does not offer it in NTSC or have the ability of converting it). From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-NOV-1995 10:51:53.59 To: MICROARCHIVE CC: Subj: Re: Microwave fixation, actually continued comment Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Thu, 2 Nov 1995 08:56:28 -0600 To: Microscopy@Sparc5.Microscopy.Com From: baskin@biosci.mbp.missouri.edu (Tobias Baskin) Subject: Re: Microwave fixation, actually continued comment Greetings, John Mansfield wrote: >OK, so Gary Login's post was not commercially based. I'm sorry. I was >going to send the question to Nestor Zaluzec only but I got distracted and >sent it to the list by mistake. However, I think it does bear some further >comment and/or discussion. >This list is getting very large and there are now many cases of messages >that should not be sent to all subscribers without there being some >indication that a large number of them want to see the responses. It >really shouldnt have been posted to the list at all, and my reasoning for >that is below. > >Net etiquette (or Netiquette as it is typically known ) says that if there >is a question in a mailing list or newsgroup then the correct response is >to send a message to the original poster. ... I disagree completely. THere is no such thing as ONE and only one proper response. Instead, there are many kinds of responses. Although some responses are likely to be really speciallized, many other responses are likely to be of interest to many more than the poster. In the case in point, its obvious from the postings that microwave fixation is of concern to many, even in passing. It's much more efficient, spontaneous and interesting for the responses of a general nature to be posted directly to the group. They all have subject lines. IF you don't want to read a post about microwave fixation-hit the delete key. Just my two cents, Tobias Baskin - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - ___ ____ ^ ____ _____ Tobias I. Baskin / \ / / \ / \ / University of Missouri / | / / \ / / Biological Sciences /___ / /__ /_____\ / /__ 109 Tucker Hall / / / \ ( / Columbia, MO 65211 USA / / / \ \ / voice: 314-882-0173 / /____ / \ \____/ /_____ fax: 314-882-0123 From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-NOV-1995 12:01:12.72 To: MICROARCHIVE CC: Subj: 50 nanometer resolution standard Date: Thu, 2 Nov 1995 09:52:40 -0600 (CST) From: "Daniel L. Callahan" To: microscopy cc: "Daniel L. Callahan" Subject: 50 nanometer resolution standard Message-ID: MIME-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII All: I've had a recent request for a resolution standard in the 50-100 nm range. I believe that this is for a relatively low-resolution SEMPA system, but could be mistaken. This seems to be awkwardly between the <5 nm standards used for modern SAM resolution and a good optical microscope standard. I was hoping that someone either might have an inspiring though or perhaps an idea from the days when SEM resolution wasn't quite what it is today? Please respond directly; if anyone expresses interest I will summarize responses. Daniel L. Callahan Department of Mechanical Engg. and Materials Science Rice University dlc@owlnet.rice.edu From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-NOV-1995 12:01:14.35 To: MICROARCHIVE CC: Subj: 50 nanometer resolution standard Date: Thu, 2 Nov 1995 09:52:40 -0600 (CST) From: "Daniel L. Callahan" To: microscopy cc: "Daniel L. Callahan" Subject: 50 nanometer resolution standard Message-ID: MIME-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII All: I've had a recent request for a resolution standard in the 50-100 nm range. I believe that this is for a relatively low-resolution SEMPA system, but could be mistaken. This seems to be awkwardly between the <5 nm standards used for modern SAM resolution and a good optical microscope standard. I was hoping that someone either might have an inspiring though or perhaps an idea from the days when SEM resolution wasn't quite what it is today? Please respond directly; if anyone expresses interest I will summarize responses. Daniel L. Callahan Department of Mechanical Engg. and Materials Science Rice University dlc@owlnet.rice.edu From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-NOV-1995 13:02:41.87 To: MICROARCHIVE CC: Subj: Re: Microwave fixation, actually continued comment Message-Id: <199511021539.JAA26587@bcm.tmc.edu> X-Sender: joiner@bcm.tmc.edu X-Mailer: Windows Eudora Version 2.1.1 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Thu, 02 Nov 1995 10:36:59 -0600 To: microscopy@Sparc5.Microscopy.Com From: "Joiner Cartwright, Jr., Ph.D." Subject: Re: Microwave fixation, actually continued comment At 11:34 PM 11/1/95 -0400, John F. Mansfield wrote: >Net etiquette (or Netiquette as it is typically known says that if there >is a question in a mailing list or newsgroup then the correct response is >to send a message to the original poster. Often a poster will say "...... I will summarize to the net/list....." ************* It seems to me that your approach would hamper the free exchange that this list is so good at and would be a lot more work than merely erasing the superfluous replies; it's not that difficult. People seem to be pretty skilled at crafting their SUBJECT: headings so you don't have to read everything posted. * * Joiner Cartwright, Jr. * * From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-NOV-1995 14:41:35.03 To: MICROARCHIVE CC: Subj: Re: Structure info from XRD -- amorphous material From: William Tivol Message-Id: <199511021831.NAA02201@wadsworth.ph.albany.edu> Subject: Re: Structure info from XRD -- amorphous material To: walcksd@ml.wpafb.af.mil (Scott.D.Walck@ml.wpafb.af.mil, WL/MLBT) Date: Thu, 2 Nov 1995 13:31:01 -0500 (EST) Cc: microscopy@Sparc5.Microscopy.Com In-Reply-To: <951031081338.726@cliff.ml.wpafb.af.mil.0> from "Scott.D.Walck@ml.wpafb.af.mil, WL/MLBT" at Oct 31, 95 08:13:38 am X-Mailer: ELM [version 2.4 PL21] Content-Type: text Content-Length: 456 > If you > are getting fringe contrast in the TEM, then the particles are not amorphous. > Dear Scott, Are the fringes observed when an opaque material with a flat edge occludes a light source the same as those seen in the EM at the edge of a particle? If so, then the crystallinity of the particle has no bearing on whether the fringes are there. I think all that's required is an abrupt change in mass density (or opacity). Yours, Bill Tivol From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-NOV-1995 15:11:54.09 To: MICROARCHIVE CC: Subj: Re: Reduced Osmium From: William Tivol Message-Id: <199511021844.NAA03672@wadsworth.ph.albany.edu> Subject: Re: Reduced Osmium To: Paul.Webster@quickmail.yale.edu (Paul Webster) Date: Thu, 2 Nov 1995 13:44:04 -0500 (EST) Cc: microscopy@Sparc5.Microscopy.Com In-Reply-To: from "Paul Webster" at Nov 1, 95 09:57:41 am X-Mailer: ELM [version 2.4 PL21] Content-Type: text Content-Length: 484 > > Dear colleagues, > can someone help by providing a good recipe for reducing osmium tetroxide to > help increase contrast in epoxy resin - embedded biological material? Is > potassium ferri-, or ferro- cyanide the best? I thought I knew the answer but a > post-doc here has given me doubts so we have a small wager riding on this. > Dear Paul, To reduce osmium, one must oxidise the other reagent, so ferri (Fe2+) should be better than ferro (Fe3+). Yours, Bill Tivol From: SMTP%"Microscopy-request@Sparc5.Microscopy.Com" 2-NOV-1995 16:12:46.15 To: MICROARCHIVE CC: Subj: Re: PAL Laserdisk to anything Message-Id: <199511021916.NAA19306@bcm.tmc.edu> X-Sender: joiner@bcm.tmc.edu X-Mailer: Windows Eudora Version 2.1.1 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Thu, 02 Nov 1995 14:13:49 -0600 To: microscopy@Sparc5.Microscopy.Com From: "Joiner Cartwright, Jr., Ph.D." Subject: Re: PAL Laserdisk to anything At 08:46 AM 11/2/95 -500, Richard Edelmann wrote: > Technically not a microscopy question but an imaging question. > > Does anyone out there know of any source for converting a european >PAL format laserdisk (of microscopic images and audio) into any >common North American format (i.e. NTSC Video tape or CD-ROM) so we >can use the disk as a teaching tool? (The company that sells the >disk does not offer it in NTSC or have the ability of converting it). > > ************** Richard - Look in your yellow pages under <